ES2255281T3 - Sobreexpresion de genes de fitasa en sistmas de levadura. - Google Patents
Sobreexpresion de genes de fitasa en sistmas de levadura.Info
- Publication number
- ES2255281T3 ES2255281T3 ES99935340T ES99935340T ES2255281T3 ES 2255281 T3 ES2255281 T3 ES 2255281T3 ES 99935340 T ES99935340 T ES 99935340T ES 99935340 T ES99935340 T ES 99935340T ES 2255281 T3 ES2255281 T3 ES 2255281T3
- Authority
- ES
- Spain
- Prior art keywords
- protein
- polypeptide
- sistmas
- levadura
- fitasa
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 230000002018 overexpression Effects 0.000 title 1
- 229920001184 polypeptide Polymers 0.000 abstract 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 4
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 4
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 3
- 101150050411 appA gene Proteins 0.000 abstract 3
- 108010011619 6-Phytase Proteins 0.000 abstract 2
- 229940085127 phytase Drugs 0.000 abstract 2
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Polymers & Plastics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Birds (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
- Management, Administration, Business Operations System, And Electronic Commerce (AREA)
- Devices For Dispensing Beverages (AREA)
Abstract
Un método de producción de fitasa en levadura que comprende: proporcionar un gen appA aislado a partir de células bacterianas, codificando dicho gen appA una proteína o polipéptido con actividad fitasa, expresar dicho gen appA en una cepa de levadura, y aislar la proteína o polipéptido expresado, teniendo dicha proteína o polipéptido una termoestabilidad aumentada en comparación con la de dicha proteína o polipéptido expresado en una célula hospedadora no de levadura.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US104769 | 1998-06-25 | ||
US09/104,769 US6451572B1 (en) | 1998-06-25 | 1998-06-25 | Overexpression of phytase genes in yeast systems |
PCT/US1999/014106 WO1999067398A2 (en) | 1998-06-25 | 1999-06-23 | Overexpression of phytase genes in yeast systems |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2255281T3 true ES2255281T3 (es) | 2006-06-16 |
ES2255281T5 ES2255281T5 (es) | 2013-06-04 |
Family
ID=22302276
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES99935340T Expired - Lifetime ES2255281T5 (es) | 1998-06-25 | 1999-06-23 | Sobreexpresión de genes de fitasa en sistemas de levadura |
ES06075318.3T Expired - Lifetime ES2560661T3 (es) | 1998-06-25 | 1999-06-23 | Sobreexpresión de genes de fitasa en sistemas de levadura |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES06075318.3T Expired - Lifetime ES2560661T3 (es) | 1998-06-25 | 1999-06-23 | Sobreexpresión de genes de fitasa en sistemas de levadura |
Country Status (18)
Country | Link |
---|---|
US (6) | US6451572B1 (es) |
EP (3) | EP1688500B1 (es) |
JP (2) | JP2002518052A (es) |
KR (1) | KR100870886B1 (es) |
CN (1) | CN100365126C (es) |
AT (1) | ATE317904T1 (es) |
AU (1) | AU772071B2 (es) |
BR (1) | BRPI9911549B1 (es) |
CA (1) | CA2332180C (es) |
CZ (1) | CZ301680B6 (es) |
DE (1) | DE69929886C5 (es) |
DK (2) | DK1688500T3 (es) |
ES (2) | ES2255281T5 (es) |
HU (1) | HUP0102800A2 (es) |
MX (1) | MX258391B (es) |
PL (1) | PL345408A1 (es) |
PT (1) | PT1688500E (es) |
WO (1) | WO1999067398A2 (es) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011141613A2 (es) | 2011-08-08 | 2011-11-17 | Fertinagro Nutrientes, S.L. | Nueva fitasa, procedimiento de obtencion y utilizacion de la misma |
Families Citing this family (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6720014B1 (en) * | 1997-08-13 | 2004-04-13 | Diversa Corporation | Phytase-containing foodstuffs and methods of making and using them |
US6451572B1 (en) * | 1998-06-25 | 2002-09-17 | Cornell Research Foundation, Inc. | Overexpression of phytase genes in yeast systems |
US6841370B1 (en) | 1999-11-18 | 2005-01-11 | Cornell Research Foundation, Inc. | Site-directed mutagenesis of Escherichia coli phytase |
KR100375673B1 (ko) * | 2000-01-29 | 2003-03-15 | 대한민국 | 피타제 생산 효모 균주를 함유하는 사료용 조성물 |
US6737262B1 (en) * | 2000-07-11 | 2004-05-18 | Robert I. Bolla | Animal feed containing polypeptides |
JP3813055B2 (ja) * | 2000-07-26 | 2006-08-23 | ソレイ リミテッド ライアビリティ カンパニー | 高純度植物タンパク材料の製造方法 |
EP1381345B1 (en) * | 2001-03-23 | 2008-07-09 | Advanced Bionutrition Corporation | Delivery of disease control in aquaculture using yeasts containing bioactive proteins |
BRPI0213813B1 (pt) | 2001-10-31 | 2018-11-21 | Cornell Res Foundation Inc | método para aprimorar o valor nutricional de uma ração consumida por um animal monogástrico e ração |
MY139056A (en) * | 2001-12-28 | 2009-08-28 | Ab Enzymes Gmbh | Microbially-expressed thermotolerant phytase for animal feed |
TW200305430A (en) * | 2001-12-28 | 2003-11-01 | Syngenta Participations Ag | Thermotolerant phytase for animal feed |
CN100398645C (zh) * | 2002-08-16 | 2008-07-02 | 广东肇庆星湖生物科技股份有限公司 | 用化学合成和分子进化获得酵母表达高比活植酸酶基因 |
CN100354421C (zh) * | 2002-08-16 | 2007-12-12 | 广东肇庆星湖生物科技股份有限公司 | 在甲醇酵母中高表达的耐高温植酸酶基因 |
WO2004024885A2 (en) | 2002-09-13 | 2004-03-25 | Cornell Research Foundation, Inc. | Using mutations to improve aspergillus phytases |
AU2003291063A1 (en) * | 2002-11-19 | 2004-06-15 | Board Of Trustees Operating Michigan State University | Antioxidant and antimicrobial agents and methods of use thereof |
US20070184521A1 (en) * | 2003-07-03 | 2007-08-09 | Alissa Jourdan | Novel phytase and gene |
US7276362B2 (en) | 2004-01-30 | 2007-10-02 | Roche Diagnostics Operations, Inc. | Recombinant histidine-tagged inosine monophosphate dehydrogenase polypeptides |
US20070224126A1 (en) * | 2004-06-01 | 2007-09-27 | Therese Dufresne | Index and Method of use of Adapted Food Compositions for Dysphagic Persons |
US20080095888A1 (en) * | 2004-08-02 | 2008-04-24 | Grant Vandenberg | Nutritional Ingredient Containing Bioavailable Mineral Nutrients |
KR100754242B1 (ko) | 2005-08-29 | 2007-09-03 | (주)진바이오텍 | 효모 유래 프로모터를 이용한 대장균 유래 파이타제의제조방법 |
US7919297B2 (en) | 2006-02-21 | 2011-04-05 | Cornell Research Foundation, Inc. | Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase |
US8540984B2 (en) * | 2006-08-03 | 2013-09-24 | Cornell Research Foundation, Inc. | Phytases with improved thermal stability |
US8192734B2 (en) | 2007-07-09 | 2012-06-05 | Cornell University | Compositions and methods for bone strengthening |
EP2258854A1 (en) * | 2009-05-20 | 2010-12-08 | FH Campus Wien | Eukaryotic host cell comprising an expression enhancer |
HUE042897T2 (hu) | 2013-03-08 | 2019-07-29 | Biogrammatics Inc | Élesztõpromóterek fehérje-expresszióhoz |
DK3594351T3 (da) | 2013-03-08 | 2022-01-03 | Keck Graduate Inst Of Applied Life Sciences | Gærpromotorer fra pichia pastoris |
WO2019023034A2 (en) * | 2017-07-27 | 2019-01-31 | Locus Agriculture Ip Company, Llc | EFFICIENT PRODUCTION OF PICHIA YEASTS AND USE THEREOF TO IMPROVE PLANT AND ANIMAL HEALTH |
EP3453719A1 (en) | 2017-09-07 | 2019-03-13 | Huvepharma Eood | New thermostable phytases with high catalytic efficacy |
CN109601822B (zh) * | 2019-01-22 | 2022-11-08 | 北京三强核力辐射工程技术有限公司 | 一种生物碱协同辐照降解动物源性食品中抗生素的方法 |
CN110029120B (zh) * | 2019-03-19 | 2022-03-01 | 青岛蔚蓝生物集团有限公司 | 一种植酸酶高产菌株及其应用 |
CN112680464B (zh) * | 2020-12-10 | 2022-04-26 | 南京农业大学 | 一种单甲基砷和三价锑氧化酶基因arsV及其编码的蛋白和应用 |
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1999
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- 1999-06-23 EP EP06075318.3A patent/EP1688500B1/en not_active Revoked
- 1999-06-23 WO PCT/US1999/014106 patent/WO1999067398A2/en active IP Right Grant
- 1999-06-23 DK DK06075318.3T patent/DK1688500T3/en active
- 1999-06-23 HU HU0102800A patent/HUP0102800A2/hu unknown
- 1999-06-23 KR KR1020007014601A patent/KR100870886B1/ko not_active IP Right Cessation
- 1999-06-23 AT AT99935340T patent/ATE317904T1/de not_active IP Right Cessation
- 1999-06-23 AU AU50837/99A patent/AU772071B2/en not_active Expired
- 1999-06-23 CN CNB998098930A patent/CN100365126C/zh not_active Expired - Lifetime
- 1999-06-23 CA CA2332180A patent/CA2332180C/en not_active Expired - Lifetime
- 1999-06-23 ES ES99935340T patent/ES2255281T5/es not_active Expired - Lifetime
- 1999-06-23 JP JP2000556038A patent/JP2002518052A/ja not_active Withdrawn
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- 1999-06-23 BR BRPI9911549A patent/BRPI9911549B1/pt active IP Right Grant
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- 1999-06-23 EP EP15200497.4A patent/EP3020815A1/en not_active Withdrawn
- 1999-06-23 DK DK99935340.2T patent/DK1090129T4/da active
- 1999-06-23 MX MXPA00012794 patent/MX258391B/es active IP Right Grant
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011141613A2 (es) | 2011-08-08 | 2011-11-17 | Fertinagro Nutrientes, S.L. | Nueva fitasa, procedimiento de obtencion y utilizacion de la misma |
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