AR003407A1 - Procedimiento de clonacion de un acido nucleico que codifica una proteina con actividad de delta-5,7-esterol, delta-7-reductasa, procedimiento de preparacion de la proteina, procemiento de reduccion in vitro de un esterol insaturado, procedimiento de produccion de pregnenolona, cepa de levadura transformada y metodo de diagnostico in vitro para la deteccion de la deficiencia de delta-5, 7-esterol, delta-7-reductasa - Google Patents

Procedimiento de clonacion de un acido nucleico que codifica una proteina con actividad de delta-5,7-esterol, delta-7-reductasa, procedimiento de preparacion de la proteina, procemiento de reduccion in vitro de un esterol insaturado, procedimiento de produccion de pregnenolona, cepa de levadura transformada y metodo de diagnostico in vitro para la deteccion de la deficiencia de delta-5, 7-esterol, delta-7-reductasa

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Publication number
AR003407A1
AR003407A1 ARP960101380A AR10138096A AR003407A1 AR 003407 A1 AR003407 A1 AR 003407A1 AR P960101380 A ARP960101380 A AR P960101380A AR 10138096 A AR10138096 A AR 10138096A AR 003407 A1 AR003407 A1 AR 003407A1
Authority
AR
Argentina
Prior art keywords
delta
sterol
procedure
reductase
protein
Prior art date
Application number
ARP960101380A
Other languages
English (en)
Original Assignee
Aventis Pharma Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR9501723A external-priority patent/FR2730494B1/fr
Priority claimed from FR9506517A external-priority patent/FR2734839B1/fr
Application filed by Aventis Pharma Sa filed Critical Aventis Pharma Sa
Publication of AR003407A1 publication Critical patent/AR003407A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/02—Dehydrogenating; Dehydroxylating

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Una secuencia de ADNc que codifica una proteína de A, thaliana que posee actividad de delta-5,7-esterol, delta-7-reductasa (secuencia SEC ID N°1);proteína delta-7RED; anticuerpo dirigido contra dicha proteína, vector de expresión, célulahos pedante, un procedimiento de clonación del ácido nucleico,procedimiento de preparación de la proteína, un procediento de reducción in vitro e in vivo de un esterol insaturado en la posición C-7, un procedimientode producción de pregnenolona,cepa de levadura transformada y método de adición de la deficiencia de delta-5,7-esterol, delta-7-reductasa.
ARP960101380A 1995-02-15 1996-02-14 Procedimiento de clonacion de un acido nucleico que codifica una proteina con actividad de delta-5,7-esterol, delta-7-reductasa, procedimiento de preparacion de la proteina, procemiento de reduccion in vitro de un esterol insaturado, procedimiento de produccion de pregnenolona, cepa de levadura transformada y metodo de diagnostico in vitro para la deteccion de la deficiencia de delta-5, 7-esterol, delta-7-reductasa AR003407A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9501723A FR2730494B1 (fr) 1995-02-15 1995-02-15 Sequence d'adn codant pour une proteine d'a. thaliana ayant une activite delta-5,7 sterol, delta-7 reductase, proteine delta7-red, procede de production, souches de levures transformees, applications
FR9506517A FR2734839B1 (fr) 1995-06-01 1995-06-01 Sequence d'adn codant pour une proteine d'a. thaliana ayant une activite delta-5,7 sterol, delta-7 reductase, proteine delta-7red, procede de production, souches de levures transformees, applications.

Publications (1)

Publication Number Publication Date
AR003407A1 true AR003407A1 (es) 1998-08-05

Family

ID=26231759

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP960101380A AR003407A1 (es) 1995-02-15 1996-02-14 Procedimiento de clonacion de un acido nucleico que codifica una proteina con actividad de delta-5,7-esterol, delta-7-reductasa, procedimiento de preparacion de la proteina, procemiento de reduccion in vitro de un esterol insaturado, procedimiento de produccion de pregnenolona, cepa de levadura transformada y metodo de diagnostico in vitro para la deteccion de la deficiencia de delta-5, 7-esterol, delta-7-reductasa
ARP060102688A AR053931A2 (es) 1995-02-15 2006-06-22 Secuencia de adn que codifica una proteina de a. thaliana que posee actividad de delta -5,7-esterol, delta-7-reductasa, proteina delta-7red

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP060102688A AR053931A2 (es) 1995-02-15 2006-06-22 Secuencia de adn que codifica una proteina de a. thaliana que posee actividad de delta -5,7-esterol, delta-7-reductasa, proteina delta-7red

Country Status (24)

Country Link
US (3) US5759801A (es)
EP (1) EP0727489B1 (es)
JP (1) JP4082469B2 (es)
KR (1) KR100422293B1 (es)
CN (2) CN1216995C (es)
AR (2) AR003407A1 (es)
AT (1) ATE457354T1 (es)
BR (1) BRPI9600729B8 (es)
CA (1) CA2169524C (es)
CZ (1) CZ294860B6 (es)
DE (1) DE69638123D1 (es)
DK (1) DK0727489T3 (es)
ES (1) ES2339833T3 (es)
FI (1) FI120877B (es)
HR (1) HRP960078B1 (es)
HU (1) HU220587B1 (es)
IL (4) IL116872A (es)
PL (1) PL185569B1 (es)
PT (1) PT727489E (es)
RU (1) RU2242517C2 (es)
SI (1) SI0727489T1 (es)
SK (1) SK283656B6 (es)
TR (1) TR199600116A2 (es)
UA (1) UA72172C2 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2258571A1 (en) * 1996-06-21 1997-12-24 The General Hospital Corporation Plant sterol reductases and uses thereof
DE19739940A1 (de) * 1997-09-11 1999-03-18 Hartmut Prof Dr Med Glossmann Protein mit Aminosäuresequenz der DELTA7-Sterolreduktase, isoliertes cDNA-Molekül, das für das Protein kodiert, rekombinanter DNA-Vektor und biologisch aktive Zusammensetzungen, die das Protein oder das cDNA-Molekül enthalten
FR2774393B1 (fr) * 1998-02-05 2000-03-24 Hoechst Marion Roussel Inc Souches de levure ayant le gene atf2 interrompu et leurs applications
US6465717B1 (en) * 1998-11-20 2002-10-15 E. I. Du Pont De Nemours And Company Sterol metabolism enzymes
FR2812884B1 (fr) * 2000-08-08 2002-11-08 Aventis Pharma Sa Levures modifiees et utilisations, notamment pour la production de derives steroidiens
FR2820145B1 (fr) 2001-01-31 2004-01-23 Aventis Pharma Sa Souche de levure produisant des steroides de facon autonome
US7193169B2 (en) * 2003-10-29 2007-03-20 Alcon, Inc. Ergonomic footswitch
FR2869914B1 (fr) 2004-05-06 2012-11-09 Aventis Pharma Sa Souches de levure produisant du cholesterol et leurs applications
AU2006227634B2 (en) * 2005-03-16 2010-07-08 Metabolix, Inc. Chemically inducible expression of biosynthetic pathways
KR100672006B1 (ko) * 2005-08-03 2007-01-19 길병옥 폐자재를 이용한 형강의 제조방법 및 그 제조방법에 의해생산된 형강
AU2008340017B2 (en) 2007-12-21 2012-04-05 Novartis Ag Organic compounds
DE102009022772A1 (de) 2009-05-21 2010-11-25 Organobalance Gmbh Mikroorganismus zur Expression eines humanen Membranproteins
FR2961218A1 (fr) 2010-06-10 2011-12-16 Sanofi Aventis Methode de detection de composes modulateurs du metabolisme du cholesterol
CN102286053B (zh) * 2011-06-20 2012-09-05 河北联合大学 孕甾烯醇酮吡咯化合物及其制备方法
TWI654300B (zh) * 2013-06-17 2019-03-21 賽諾菲公司 用於細胞色素ｐ４５０單加氧酶生物催化作用之全細胞系統
CN110903993A (zh) * 2019-12-20 2020-03-24 河北兰升生物科技有限公司 一种产生菜籽甾醇的酿酒酵母工程菌及其构建方法和应用
CN112813129B (zh) * 2021-02-05 2023-09-08 江南大学 利用区室化提高酵母菌中7-脱氢胆固醇产量的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1042567A (zh) * 1988-09-23 1990-05-30 吉斯特-布罗卡迪斯公司 类固醇多步氧化方法和所用的遗传工程细胞
JP2645130B2 (ja) * 1989-03-31 1997-08-25 日清製粉株式会社 ステロイド誘導体
US5547868A (en) * 1993-06-09 1996-08-20 Regents Of The University Of California Cholesterol disposal fusion enzymes

Also Published As

Publication number Publication date
SK18896A3 (en) 1996-10-02
IL116872A0 (en) 1996-07-23
BRPI9600729B8 (pt) 2021-07-06
JP4082469B2 (ja) 2008-04-30
DK0727489T3 (da) 2010-05-31
BRPI9600729A (pt) 1997-12-23
RU2242517C2 (ru) 2004-12-20
FI120877B (fi) 2010-04-15
KR960031607A (ko) 1996-09-17
TR199600116A2 (tr) 1996-08-21
PL185569B1 (pl) 2003-06-30
FI960663A0 (fi) 1996-02-14
US5965417A (en) 1999-10-12
IL116872A (en) 2004-12-15
ES2339833T3 (es) 2010-05-25
CN1664107A (zh) 2005-09-07
CN1145953A (zh) 1997-03-26
HUP9600325A3 (en) 1999-07-28
AR053931A2 (es) 2007-05-23
IL162476A0 (en) 2005-11-20
CZ294860B6 (cs) 2005-04-13
HRP960078A2 (es) 1998-02-28
ATE457354T1 (de) 2010-02-15
FI960663A (fi) 1996-08-16
CA2169524C (fr) 2012-05-01
SK283656B6 (sk) 2003-11-04
US5989881A (en) 1999-11-23
BRPI9600729B1 (pt) 2017-06-20
HRP960078B1 (en) 2011-11-30
DE69638123D1 (de) 2010-03-25
JPH08289793A (ja) 1996-11-05
SI0727489T1 (sl) 2010-05-31
CZ43296A3 (en) 1996-12-11
HU220587B1 (hu) 2002-03-28
PL312828A1 (en) 1996-08-19
KR100422293B1 (ko) 2004-06-14
IL162477A0 (en) 2005-11-20
EP0727489A1 (fr) 1996-08-21
EP0727489B1 (fr) 2010-02-10
CA2169524A1 (fr) 1996-08-16
PT727489E (pt) 2010-04-27
US5759801A (en) 1998-06-02
CN1216995C (zh) 2005-08-31
HUP9600325A1 (en) 1996-11-28
IL162476A (en) 2005-12-18
UA72172C2 (uk) 2005-02-15

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