DK0727489T3 - DNA-sekvens, der koder for et A. thaliana protein med delta-5,7-sterol, delta-7-reduktase-aktivitet, delta-7Red-protein, fremgangsmåde til fremstilling, transformerede gærstammer, anvendelser - Google Patents

DNA-sekvens, der koder for et A. thaliana protein med delta-5,7-sterol, delta-7-reduktase-aktivitet, delta-7Red-protein, fremgangsmåde til fremstilling, transformerede gærstammer, anvendelser

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Publication number
DK0727489T3
DK0727489T3 DK96400301.6T DK96400301T DK0727489T3 DK 0727489 T3 DK0727489 T3 DK 0727489T3 DK 96400301 T DK96400301 T DK 96400301T DK 0727489 T3 DK0727489 T3 DK 0727489T3
Authority
DK
Denmark
Prior art keywords
delta
protein
sterol
preparation
sequence encoding
Prior art date
Application number
DK96400301.6T
Other languages
English (en)
Inventor
Xavier Chenivesse
Catherine Duport
Eric Lecain
Denis Pompon
Original Assignee
Aventis Pharma Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR9501723A external-priority patent/FR2730494B1/fr
Priority claimed from FR9506517A external-priority patent/FR2734839B1/fr
Application filed by Aventis Pharma Sa filed Critical Aventis Pharma Sa
Application granted granted Critical
Publication of DK0727489T3 publication Critical patent/DK0727489T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/02—Dehydrogenating; Dehydroxylating
DK96400301.6T 1995-02-15 1996-02-14 DNA-sekvens, der koder for et A. thaliana protein med delta-5,7-sterol, delta-7-reduktase-aktivitet, delta-7Red-protein, fremgangsmåde til fremstilling, transformerede gærstammer, anvendelser DK0727489T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9501723A FR2730494B1 (fr) 1995-02-15 1995-02-15 Sequence d'adn codant pour une proteine d'a. thaliana ayant une activite delta-5,7 sterol, delta-7 reductase, proteine delta7-red, procede de production, souches de levures transformees, applications
FR9506517A FR2734839B1 (fr) 1995-06-01 1995-06-01 Sequence d'adn codant pour une proteine d'a. thaliana ayant une activite delta-5,7 sterol, delta-7 reductase, proteine delta-7red, procede de production, souches de levures transformees, applications.

Publications (1)

Publication Number Publication Date
DK0727489T3 true DK0727489T3 (da) 2010-05-31

Family

ID=26231759

Family Applications (1)

Application Number Title Priority Date Filing Date
DK96400301.6T DK0727489T3 (da) 1995-02-15 1996-02-14 DNA-sekvens, der koder for et A. thaliana protein med delta-5,7-sterol, delta-7-reduktase-aktivitet, delta-7Red-protein, fremgangsmåde til fremstilling, transformerede gærstammer, anvendelser

Country Status (24)

Country Link
US (3) US5759801A (da)
EP (1) EP0727489B1 (da)
JP (1) JP4082469B2 (da)
KR (1) KR100422293B1 (da)
CN (2) CN1664107A (da)
AR (2) AR003407A1 (da)
AT (1) ATE457354T1 (da)
BR (1) BRPI9600729B8 (da)
CA (1) CA2169524C (da)
CZ (1) CZ294860B6 (da)
DE (1) DE69638123D1 (da)
DK (1) DK0727489T3 (da)
ES (1) ES2339833T3 (da)
FI (1) FI120877B (da)
HR (1) HRP960078B1 (da)
HU (1) HU220587B1 (da)
IL (4) IL162476A (da)
PL (1) PL185569B1 (da)
PT (1) PT727489E (da)
RU (1) RU2242517C2 (da)
SI (1) SI0727489T1 (da)
SK (1) SK283656B6 (da)
TR (1) TR199600116A2 (da)
UA (1) UA72172C2 (da)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997048793A1 (en) * 1996-06-21 1997-12-24 The General Hospital Corporation Plant sterol reductases and uses thereof
DE19739940A1 (de) * 1997-09-11 1999-03-18 Hartmut Prof Dr Med Glossmann Protein mit Aminosäuresequenz der DELTA7-Sterolreduktase, isoliertes cDNA-Molekül, das für das Protein kodiert, rekombinanter DNA-Vektor und biologisch aktive Zusammensetzungen, die das Protein oder das cDNA-Molekül enthalten
FR2774393B1 (fr) * 1998-02-05 2000-03-24 Hoechst Marion Roussel Inc Souches de levure ayant le gene atf2 interrompu et leurs applications
US6465717B1 (en) * 1998-11-20 2002-10-15 E. I. Du Pont De Nemours And Company Sterol metabolism enzymes
FR2812884B1 (fr) * 2000-08-08 2002-11-08 Aventis Pharma Sa Levures modifiees et utilisations, notamment pour la production de derives steroidiens
FR2820145B1 (fr) * 2001-01-31 2004-01-23 Aventis Pharma Sa Souche de levure produisant des steroides de facon autonome
US7193169B2 (en) * 2003-10-29 2007-03-20 Alcon, Inc. Ergonomic footswitch
FR2869914B1 (fr) 2004-05-06 2012-11-09 Aventis Pharma Sa Souches de levure produisant du cholesterol et leurs applications
US7732680B2 (en) * 2005-03-16 2010-06-08 Metabolix, Inc. Chemically inducible expression of biosynthetic pathways
KR100672006B1 (ko) * 2005-08-03 2007-01-19 길병옥 폐자재를 이용한 형강의 제조방법 및 그 제조방법에 의해생산된 형강
PT2227549E (pt) 2007-12-21 2015-10-08 Novartis Ag Sistema de seleção para cultura de células eucarióticas com base num gene de recetor de folato ligado à membrana
DE102009022772A1 (de) 2009-05-21 2010-11-25 Organobalance Gmbh Mikroorganismus zur Expression eines humanen Membranproteins
FR2961218A1 (fr) * 2010-06-10 2011-12-16 Sanofi Aventis Methode de detection de composes modulateurs du metabolisme du cholesterol
CN102286053B (zh) * 2011-06-20 2012-09-05 河北联合大学 孕甾烯醇酮吡咯化合物及其制备方法
KR20160019451A (ko) 2013-06-17 2016-02-19 사노피 시토크롬 p450 모노옥시게나제 생체촉매작용을 위한 전세포 시스템
CN110903993A (zh) * 2019-12-20 2020-03-24 河北兰升生物科技有限公司 一种产生菜籽甾醇的酿酒酵母工程菌及其构建方法和应用
CN112813129B (zh) * 2021-02-05 2023-09-08 江南大学 利用区室化提高酵母菌中7-脱氢胆固醇产量的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1042567A (zh) * 1988-09-23 1990-05-30 吉斯特-布罗卡迪斯公司 类固醇多步氧化方法和所用的遗传工程细胞
JP2645130B2 (ja) * 1989-03-31 1997-08-25 日清製粉株式会社 ステロイド誘導体
US5547868A (en) * 1993-06-09 1996-08-20 Regents Of The University Of California Cholesterol disposal fusion enzymes

Also Published As

Publication number Publication date
IL162477A0 (en) 2005-11-20
KR960031607A (ko) 1996-09-17
ATE457354T1 (de) 2010-02-15
IL162476A0 (en) 2005-11-20
CA2169524C (fr) 2012-05-01
BRPI9600729B1 (pt) 2017-06-20
HRP960078B1 (en) 2011-11-30
EP0727489B1 (fr) 2010-02-10
SK18896A3 (en) 1996-10-02
AR003407A1 (es) 1998-08-05
CN1664107A (zh) 2005-09-07
ES2339833T3 (es) 2010-05-25
CN1216995C (zh) 2005-08-31
PT727489E (pt) 2010-04-27
HUP9600325A3 (en) 1999-07-28
JPH08289793A (ja) 1996-11-05
RU2242517C2 (ru) 2004-12-20
HU220587B1 (hu) 2002-03-28
IL116872A (en) 2004-12-15
IL116872A0 (en) 1996-07-23
US5759801A (en) 1998-06-02
FI960663A0 (fi) 1996-02-14
BRPI9600729B8 (pt) 2021-07-06
AR053931A2 (es) 2007-05-23
SK283656B6 (sk) 2003-11-04
CZ294860B6 (cs) 2005-04-13
CA2169524A1 (fr) 1996-08-16
HRP960078A2 (da) 1998-02-28
FI120877B (fi) 2010-04-15
US5965417A (en) 1999-10-12
HUP9600325A1 (en) 1996-11-28
CZ43296A3 (en) 1996-12-11
JP4082469B2 (ja) 2008-04-30
CN1145953A (zh) 1997-03-26
PL185569B1 (pl) 2003-06-30
TR199600116A2 (tr) 1996-08-21
DE69638123D1 (de) 2010-03-25
IL162476A (en) 2005-12-18
FI960663A (fi) 1996-08-16
BRPI9600729A (pt) 1997-12-23
PL312828A1 (en) 1996-08-19
SI0727489T1 (sl) 2010-05-31
EP0727489A1 (fr) 1996-08-21
UA72172C2 (uk) 2005-02-15
US5989881A (en) 1999-11-23
KR100422293B1 (ko) 2004-06-14

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