MX367859B - Metodos y materiales para la biosintesis de compuestos mogrosidos. - Google Patents

Metodos y materiales para la biosintesis de compuestos mogrosidos.

Info

Publication number
MX367859B
MX367859B MX2015007039A MX2015007039A MX367859B MX 367859 B MX367859 B MX 367859B MX 2015007039 A MX2015007039 A MX 2015007039A MX 2015007039 A MX2015007039 A MX 2015007039A MX 367859 B MX367859 B MX 367859B
Authority
MX
Mexico
Prior art keywords
methods
biosynthesis
materials
useful
mogroside compounds
Prior art date
Application number
MX2015007039A
Other languages
English (en)
Other versions
MX2015007039A (es
Inventor
Liu Yaoquan
Hansen Jørgen
Houghton-Larsen Jens
Panchapagesa Murali Muthuswamy
Kumar Sathish
Nicoline Rasmussen Nina
Original Assignee
Evolva Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Sa filed Critical Evolva Sa
Publication of MX2015007039A publication Critical patent/MX2015007039A/es
Publication of MX367859B publication Critical patent/MX367859B/es

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    • C—CHEMISTRY; METALLURGY
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/20—Preparation of steroids containing heterocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating
    • C12P33/08—Hydroxylating at 11 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/12—Acting on D ring
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    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
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    • C12Y—ENZYMES
    • C12Y303/00—Hydrolases acting on ether bonds (3.3)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
    • C12Y504/99033—Cucurbitadienol synthase (5.4.99.33)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13132—Squalene monooxygenase (1.14.13.132)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

La invención se refiere a métodos para producir mogrósidos con la ayuda de enzimas. En particular la invención propone diversas rutas biosintéticas útiles para la producción de mogrósido y se proporcionan enzimas útiles para la producción de mogrósido. Además, la invención proporciona huéspedes recombinantes útiles para realizar los métodos de la invención.
MX2015007039A 2012-12-04 2013-12-04 Metodos y materiales para la biosintesis de compuestos mogrosidos. MX367859B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261733220P 2012-12-04 2012-12-04
PCT/EP2013/075510 WO2014086842A1 (en) 2012-12-04 2013-12-04 Methods and materials for biosynthesis of mogroside compounds

Publications (2)

Publication Number Publication Date
MX2015007039A MX2015007039A (es) 2016-02-25
MX367859B true MX367859B (es) 2019-09-09

Family

ID=49709683

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015007039A MX367859B (es) 2012-12-04 2013-12-04 Metodos y materiales para la biosintesis de compuestos mogrosidos.

Country Status (10)

Country Link
US (4) US9932619B2 (es)
EP (1) EP2929043A1 (es)
JP (1) JP2015536157A (es)
AU (3) AU2015200486A1 (es)
BR (1) BR112015013129A2 (es)
CA (1) CA2893462A1 (es)
HK (1) HK1215053A1 (es)
MX (1) MX367859B (es)
SG (2) SG11201503757RA (es)
WO (1) WO2014086842A1 (es)

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EP3638804A1 (en) 2017-06-15 2020-04-22 Evolva SA Production of mogroside compounds in recombinant hosts
CN109385377A (zh) * 2017-08-04 2019-02-26 中国科学院天津工业生物技术研究所 用于生产葫芦二烯醇的工程菌及其制备方法与应用
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CN109679987A (zh) * 2018-09-27 2019-04-26 怀化兴科创生物技术有限公司 一个提高甜甙含量相关基因的应用
KR20210089717A (ko) 2018-11-07 2021-07-16 피르메니히 인코포레이티드 고강도 감미료의 제조 방법
CN113316394A (zh) * 2018-11-07 2021-08-27 弗门尼舍公司 制备高强度甜味剂的方法
US20210403921A1 (en) * 2018-11-09 2021-12-30 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
CN110066785B (zh) * 2019-01-30 2021-01-26 中国医学科学院药用植物研究所 一种罗汉果葫芦二烯醇合酶突变体及其构建方法和应用
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CN112903835B (zh) * 2021-01-12 2022-08-12 广州泽力医药科技有限公司 罗汉果橘红固体饮料的指纹图谱构建方法及检测方法
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BR112015013129A2 (pt) 2012-12-04 2017-07-11 Evolva Sa métodos e materiais para a biossíntese de compostos mogrosida

Also Published As

Publication number Publication date
US20150064743A1 (en) 2015-03-05
US9932619B2 (en) 2018-04-03
AU2022204430A1 (en) 2022-07-14
US20180346953A1 (en) 2018-12-06
US20150322473A1 (en) 2015-11-12
CA2893462A1 (en) 2014-06-12
SG10201704575XA (en) 2017-07-28
US10662458B2 (en) 2020-05-26
US10465222B2 (en) 2019-11-05
EP2929043A1 (en) 2015-10-14
MX2015007039A (es) 2016-02-25
AU2015200486A1 (en) 2015-03-05
HK1215053A1 (zh) 2016-08-12
WO2014086842A9 (en) 2015-06-18
SG11201503757RA (en) 2015-06-29
JP2015536157A (ja) 2015-12-21
AU2019250137A1 (en) 2019-10-31
US20180251806A1 (en) 2018-09-06
BR112015013129A2 (pt) 2017-07-11
AU2019250137B2 (en) 2022-03-24
US10011859B2 (en) 2018-07-03
WO2014086842A1 (en) 2014-06-12

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