BR112015013129A2 - métodos e materiais para a biossíntese de compostos mogrosida - Google Patents

métodos e materiais para a biossíntese de compostos mogrosida

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Publication number
BR112015013129A2
BR112015013129A2 BR112015013129A BR112015013129A BR112015013129A2 BR 112015013129 A2 BR112015013129 A2 BR 112015013129A2 BR 112015013129 A BR112015013129 A BR 112015013129A BR 112015013129 A BR112015013129 A BR 112015013129A BR 112015013129 A2 BR112015013129 A2 BR 112015013129A2
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BR
Brazil
Prior art keywords
methods
biosynthesis
mogroside
materials
present
Prior art date
Application number
BR112015013129A
Other languages
English (en)
Inventor
Houghton-Larsen Jens
Hansen Jørgen
Panchapagesa Murali Muthuswamy
Nicoline Rasmussen Nina
Kumar Sathish
Liu Yaoquan
Original Assignee
Evolva Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Sa filed Critical Evolva Sa
Publication of BR112015013129A2 publication Critical patent/BR112015013129A2/pt

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    • C12P33/00—Preparation of steroids
    • C12P33/20—Preparation of steroids containing heterocyclic rings
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
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    • C12P33/00—Preparation of steroids
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    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating
    • C12P33/08—Hydroxylating at 11 position
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    • C12P33/00—Preparation of steroids
    • C12P33/12—Acting on D ring
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    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
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    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
    • C12Y504/99033—Cucurbitadienol synthase (5.4.99.33)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13132—Squalene monooxygenase (1.14.13.132)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

resumo “métodos e materiais para a biossíntese de compostos mogrosida” a presente invenção se refere a métodos para produzir mogrosidas com o auxílio de enzimas. em particular a presente invenção propõe vários trajetos biossintéticos úteis para a produção de mogrosida e enzimas úteis para a produção de mogrosida são proporcionados. ademais, a presente invenção proporciona hospedeiros recombinantes úteis em realizar os métodos da presente invenção.
BR112015013129A 2012-12-04 2013-12-04 métodos e materiais para a biossíntese de compostos mogrosida BR112015013129A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261733220P 2012-12-04 2012-12-04
PCT/EP2013/075510 WO2014086842A1 (en) 2012-12-04 2013-12-04 Methods and materials for biosynthesis of mogroside compounds

Publications (1)

Publication Number Publication Date
BR112015013129A2 true BR112015013129A2 (pt) 2017-07-11

Family

ID=49709683

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BR112015013129A BR112015013129A2 (pt) 2012-12-04 2013-12-04 métodos e materiais para a biossíntese de compostos mogrosida

Country Status (10)

Country Link
US (4) US9932619B2 (pt)
EP (1) EP2929043A1 (pt)
JP (1) JP2015536157A (pt)
AU (3) AU2015200486A1 (pt)
BR (1) BR112015013129A2 (pt)
CA (1) CA2893462A1 (pt)
HK (1) HK1215053A1 (pt)
MX (1) MX367859B (pt)
SG (2) SG11201503757RA (pt)
WO (1) WO2014086842A1 (pt)

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CN109679987A (zh) * 2018-09-27 2019-04-26 怀化兴科创生物技术有限公司 一个提高甜甙含量相关基因的应用
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CN110066785B (zh) * 2019-01-30 2021-01-26 中国医学科学院药用植物研究所 一种罗汉果葫芦二烯醇合酶突变体及其构建方法和应用
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CN110283224B (zh) * 2019-07-16 2020-08-28 湖南华诚生物资源股份有限公司 一种罗汉果甜苷v的半合成方法
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CN117460825A (zh) * 2021-04-02 2024-01-26 银杏生物制品公司 类异戊二烯及其前体的生物合成
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CA2893462A1 (en) 2014-06-12
US10465222B2 (en) 2019-11-05
MX2015007039A (es) 2016-02-25
AU2022204430A1 (en) 2022-07-14
MX367859B (es) 2019-09-09
WO2014086842A1 (en) 2014-06-12
AU2015200486A1 (en) 2015-03-05
US20180251806A1 (en) 2018-09-06
US10011859B2 (en) 2018-07-03
SG11201503757RA (en) 2015-06-29
US20150322473A1 (en) 2015-11-12
WO2014086842A9 (en) 2015-06-18
US10662458B2 (en) 2020-05-26
HK1215053A1 (zh) 2016-08-12
US20150064743A1 (en) 2015-03-05
US20180346953A1 (en) 2018-12-06
SG10201704575XA (en) 2017-07-28
JP2015536157A (ja) 2015-12-21
AU2019250137A1 (en) 2019-10-31
AU2019250137B2 (en) 2022-03-24
EP2929043A1 (en) 2015-10-14
US9932619B2 (en) 2018-04-03

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