PH12015500737A1 - Recombinant microorganisms for producing organic acids - Google Patents

Recombinant microorganisms for producing organic acids

Info

Publication number
PH12015500737A1
PH12015500737A1 PH12015500737A PH12015500737A PH12015500737A1 PH 12015500737 A1 PH12015500737 A1 PH 12015500737A1 PH 12015500737 A PH12015500737 A PH 12015500737A PH 12015500737 A PH12015500737 A PH 12015500737A PH 12015500737 A1 PH12015500737 A1 PH 12015500737A1
Authority
PH
Philippines
Prior art keywords
organic acids
recombinant microorganisms
producing organic
microorganisms
recombinant
Prior art date
Application number
PH12015500737A
Inventor
Guettler Michael
Hanchar Robert
Kleff Susanne
Jadhav Sanchin
Original Assignee
Michigan Biotech Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Michigan Biotech Inst filed Critical Michigan Biotech Inst
Publication of PH12015500737A1 publication Critical patent/PH12015500737A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01037—Malate dehydrogenase (1.1.1.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01049—Glucose-6-phosphate dehydrogenase (1.1.1.49)

Abstract

Recombinant microorganisms that co express enzymatic glucose-6-phosphate dehydrogenase and malate dehydrogenase are generated to produce organic acids.
PH12015500737A 2012-10-02 2015-04-01 Recombinant microorganisms for producing organic acids PH12015500737A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261708998P 2012-10-02 2012-10-02
PCT/US2013/063100 WO2014055670A1 (en) 2012-10-02 2013-10-02 Recombinant microorganisms for producing organic acids

Publications (1)

Publication Number Publication Date
PH12015500737A1 true PH12015500737A1 (en) 2015-06-01

Family

ID=49486664

Family Applications (1)

Application Number Title Priority Date Filing Date
PH12015500737A PH12015500737A1 (en) 2012-10-02 2015-04-01 Recombinant microorganisms for producing organic acids

Country Status (10)

Country Link
US (2) US20140093925A1 (en)
EP (1) EP2904104B1 (en)
JP (1) JP2015530121A (en)
KR (1) KR101521045B1 (en)
CN (1) CN104854244A (en)
CA (1) CA2883407A1 (en)
IN (1) IN2015DN02873A (en)
MX (1) MX2015004180A (en)
PH (1) PH12015500737A1 (en)
WO (1) WO2014055670A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8647642B2 (en) 2008-09-18 2014-02-11 Aviex Technologies, Llc Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
KR102129379B1 (en) 2018-10-10 2020-07-02 한국과학기술원 A recombinant microorganism into which a high activity malate dehydrogenase for producing succinic acid and a method for producing succinic acid using the same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5521075A (en) 1994-12-19 1996-05-28 Michigan Biotechnology Institute Method for making succinic acid, anaerobiospirillum succiniciproducens variants for use in process and methods for obtaining variants
US5573931A (en) 1995-08-28 1996-11-12 Michigan Biotechnology Institute Method for making succinic acid, bacterial variants for use in the process, and methods for obtaining variants
CN101044245B (en) * 2004-08-27 2012-05-02 莱斯大学 Mutant e. coli strain with increased succinic acid production
US20060073577A1 (en) * 2004-09-17 2006-04-06 San Ka-Yiu High succinate producing bacteria
JP5634659B2 (en) 2004-12-22 2014-12-03 ミシガン バイオテクノロジー インスティテュート Description of US Government Assistance for Recombinant Microorganisms for High Production of Organic Acids The present invention was made with assistance from the US government at the Cooperative Agreement No. DE-FC36-02GO12001, awarded by the Department of Energy. The US government has certain rights in this invention.
CN101649300B (en) * 2009-09-07 2011-06-15 安徽丰原发酵技术工程研究有限公司 Gene engineering bacterial strain for producing L-malic acid and construction method and application thereof
EP2668281A4 (en) * 2011-01-25 2015-08-26 Cargill Inc Compositions and methods for succinate production

Also Published As

Publication number Publication date
EP2904104A1 (en) 2015-08-12
JP2015530121A (en) 2015-10-15
MX2015004180A (en) 2015-07-06
US20160326554A1 (en) 2016-11-10
IN2015DN02873A (en) 2015-09-11
KR101521045B1 (en) 2015-05-15
WO2014055670A1 (en) 2014-04-10
CN104854244A (en) 2015-08-19
EP2904104B1 (en) 2018-08-15
CA2883407A1 (en) 2014-04-10
KR20140108486A (en) 2014-09-11
US20140093925A1 (en) 2014-04-03

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