MX2022002057A - Variantes de enzima con propiedades de ester sintasa mejoradas. - Google Patents

Variantes de enzima con propiedades de ester sintasa mejoradas.

Info

Publication number
MX2022002057A
MX2022002057A MX2022002057A MX2022002057A MX2022002057A MX 2022002057 A MX2022002057 A MX 2022002057A MX 2022002057 A MX2022002057 A MX 2022002057A MX 2022002057 A MX2022002057 A MX 2022002057A MX 2022002057 A MX2022002057 A MX 2022002057A
Authority
MX
Mexico
Prior art keywords
enzyme variants
ester synthase
host cells
improved ester
recombinant host
Prior art date
Application number
MX2022002057A
Other languages
English (en)
Inventor
Costa Bernardo M Da
Kevin Holden
Noah Helman
Andrew Shumaker
Louis G Hom
Tarah S Baron
Original Assignee
Genomatica Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genomatica Inc filed Critical Genomatica Inc
Publication of MX2022002057A publication Critical patent/MX2022002057A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)
    • C12Y201/01015—Fatty-acid O-methyltransferase (2.1.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01067—Fatty-acyl-ethyl-ester synthase (3.1.1.67)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P30/00—Technologies relating to oil refining and petrochemical industry
    • Y02P30/20—Technologies relating to oil refining and petrochemical industry using bio-feedstock

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención divulga un polipéptido de éster sintasa variante que comprende la SEQ ID NO: 2, caracterizado porque el polipéptido de éster sintasa variante se modifica genéticamente por ingeniería para tener una mutación en la posición de aminoácido 317, en donde la metionina inicial con base en el sitio de inicio ATG se numera como 0, y en donde el polipéptido de éster sintasa variante tiene una actividad de éster de metilo del ácido graso mejorada en comparación con un polipéptido tipo silvestre correspondiente. En una modalidad particular de la invención la mutación en la posición de aminoácido 317 es F317W.
MX2022002057A 2012-09-14 2015-03-13 Variantes de enzima con propiedades de ester sintasa mejoradas. MX2022002057A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261701191P 2012-09-14 2012-09-14
US201261708424P 2012-10-01 2012-10-01

Publications (1)

Publication Number Publication Date
MX2022002057A true MX2022002057A (es) 2022-03-17

Family

ID=48045767

Family Applications (3)

Application Number Title Priority Date Filing Date
MX2015003305A MX366179B (es) 2012-09-14 2013-03-15 Variantes de enzima con propiedades de ester sintasa mejoradas.
MX2022002057A MX2022002057A (es) 2012-09-14 2015-03-13 Variantes de enzima con propiedades de ester sintasa mejoradas.
MX2019007766A MX2019007766A (es) 2012-09-14 2015-03-13 Variantes de enzima con propiedades de ester sintasa mejoradas.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2015003305A MX366179B (es) 2012-09-14 2013-03-15 Variantes de enzima con propiedades de ester sintasa mejoradas.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2019007766A MX2019007766A (es) 2012-09-14 2015-03-13 Variantes de enzima con propiedades de ester sintasa mejoradas.

Country Status (11)

Country Link
US (3) US9879239B2 (es)
EP (2) EP3299455A1 (es)
JP (6) JP6401703B2 (es)
KR (4) KR102493733B1 (es)
CN (2) CN112899250A (es)
AU (3) AU2013316124C1 (es)
BR (1) BR112015005782A2 (es)
CA (2) CA2885041C (es)
ES (1) ES2647412T3 (es)
MX (3) MX366179B (es)
WO (1) WO2014042693A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201501013PA (en) 2012-08-10 2015-04-29 Opx Biotechnologies Inc Microorganisms and methods for the production of fatty acids and fatty acid derived products
US20150119601A1 (en) 2013-03-15 2015-04-30 Opx Biotechnologies, Inc. Monofunctional mcr + 3-hp dehydrogenase
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
WO2015010103A2 (en) 2013-07-19 2015-01-22 Opx Biotechnologies, Inc. Microorganisms and methods for the production of fatty acids and fatty acid derived products
EP2993228B1 (en) * 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
JP2020506702A (ja) 2017-02-02 2020-03-05 カーギル インコーポレイテッド Ｃ６−ｃ１０脂肪酸誘導体を生成する遺伝子組み換え細胞
JP2020515293A (ja) * 2017-04-03 2020-05-28 ジェノマティカ， インコーポレイテッド 中鎖脂肪酸誘導体の生産のための改良された活性を有するチオエステラーゼ変種
MX2022002473A (es) * 2019-08-30 2022-08-02 Codexis Inc Variantes de lipasa modificadas geneticamente.
WO2023178193A1 (en) 2022-03-16 2023-09-21 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof

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AU7791991A (en) 1990-04-24 1991-11-11 Stratagene Methods for phenotype creation from multiple gene populations
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
BRPI0407555A (pt) 2003-02-21 2006-02-14 Kaneka Corp vetor inédito
EP1791933B1 (en) * 2004-07-16 2011-06-29 Danisco A/S Enzymatic oil-degumming method
US7897369B2 (en) * 2007-08-27 2011-03-01 Regents Of The University Of Minnesota Isoprenoid wax ester synthases, isoprenoid acyl CoA-synthetases, and uses thereof
CA3038491A1 (en) 2008-10-07 2010-04-15 REG Life Sciences, LLC Bacterial host cells engineered to express a carboxylic acid reductase and a thioesterase
WO2010075483A2 (en) 2008-12-23 2010-07-01 Ls9, Inc. Methods and compositions related to thioesterase enzymes
ES2536240T3 (es) * 2009-09-25 2015-05-21 REG Life Sciences, LLC Producción de derivados de ácidos grasos
JP2011103863A (ja) 2009-11-20 2011-06-02 National Institute For Agro-Environmental Science デオキシニバレノールの分解活性を有するタンパク質をコードする遺伝子
US8530221B2 (en) * 2010-01-14 2013-09-10 Ls9, Inc. Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
DK2601292T3 (en) 2010-08-06 2018-07-16 Biopetrolia Ab METHODS AND PRODUCTS FOR MANUFACTURING GROWERS
US8921090B2 (en) * 2011-09-27 2014-12-30 Exxonmobil Research And Engineering Company Acyl-ACP wax ester synthases

Also Published As

Publication number Publication date
JP2022051839A (ja) 2022-04-01
BR112015005782A2 (pt) 2017-08-08
AU2019202572B2 (en) 2021-11-11
AU2013316124C1 (en) 2022-01-06
US20150299679A1 (en) 2015-10-22
AU2013316124B2 (en) 2019-05-02
US20180327728A1 (en) 2018-11-15
KR102072048B1 (ko) 2020-02-03
JP2020072748A (ja) 2020-05-14
KR20230020007A (ko) 2023-02-09
AU2019202572A1 (en) 2019-05-02
CA3079799A1 (en) 2014-03-20
JP7030145B2 (ja) 2022-03-04
CN104955944A (zh) 2015-09-30
US20210309979A1 (en) 2021-10-07
WO2014042693A1 (en) 2014-03-20
CN104955944B (zh) 2021-03-09
EP3299455A1 (en) 2018-03-28
JP2020072747A (ja) 2020-05-14
JP7068362B2 (ja) 2022-05-16
EP2895602B1 (en) 2017-09-20
ES2647412T3 (es) 2017-12-21
MX2015003305A (es) 2016-01-20
JP2015528308A (ja) 2015-09-28
KR20210153158A (ko) 2021-12-16
KR20200011595A (ko) 2020-02-03
AU2022200123A1 (en) 2022-02-10
JP6401703B2 (ja) 2018-10-10
JP2019010112A (ja) 2019-01-24
KR102339617B1 (ko) 2021-12-15
MX366179B (es) 2019-07-01
CA2885041C (en) 2020-07-07
CA2885041A1 (en) 2014-03-20
KR20150056603A (ko) 2015-05-26
JP6820888B2 (ja) 2021-01-27
US10894953B2 (en) 2021-01-19
AU2013316124A1 (en) 2015-04-02
EP2895602A1 (en) 2015-07-22
CN112899250A (zh) 2021-06-04
MX2019007766A (es) 2019-10-15
US9879239B2 (en) 2018-01-30
KR102493733B1 (ko) 2023-02-06
JP2021003131A (ja) 2021-01-14

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