MX366179B - Variantes de enzima con propiedades de ester sintasa mejoradas. - Google Patents

Variantes de enzima con propiedades de ester sintasa mejoradas.

Info

Publication number
MX366179B
MX366179B MX2015003305A MX2015003305A MX366179B MX 366179 B MX366179 B MX 366179B MX 2015003305 A MX2015003305 A MX 2015003305A MX 2015003305 A MX2015003305 A MX 2015003305A MX 366179 B MX366179 B MX 366179B
Authority
MX
Mexico
Prior art keywords
enzyme variants
ester synthase
host cells
improved ester
recombinant host
Prior art date
Application number
MX2015003305A
Other languages
English (en)
Other versions
MX2015003305A (es
Inventor
M Da Costa Bernardo
holden Kevin
Helman Noah
Shumaker Andrew
G Hom Louis
S Baron Tarah
Original Assignee
Reg Life Sciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Reg Life Sciences Llc filed Critical Reg Life Sciences Llc
Publication of MX2015003305A publication Critical patent/MX2015003305A/es
Publication of MX366179B publication Critical patent/MX366179B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)
    • C12Y201/01015—Fatty-acid O-methyltransferase (2.1.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01067—Fatty-acyl-ethyl-ester synthase (3.1.1.67)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P30/00—Technologies relating to oil refining and petrochemical industry
    • Y02P30/20—Technologies relating to oil refining and petrochemical industry using bio-feedstock

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a variantes de enzima con propiedades de éster sintasa mejoradas para la producción de ésteres de ácido graso. Además se contemplan células hospederas recombinantes que expresan tales variantes, cultivos celulares que comprenden las células hospederas recombinantes y composiciones de éster de ácido graso producido por tales células hospederas recombinantes.
MX2015003305A 2012-09-14 2013-03-15 Variantes de enzima con propiedades de ester sintasa mejoradas. MX366179B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261701191P 2012-09-14 2012-09-14
US201261708424P 2012-10-01 2012-10-01
PCT/US2013/032564 WO2014042693A1 (en) 2012-09-14 2013-03-15 Enzyme variants with improved ester synthase properties

Publications (2)

Publication Number Publication Date
MX2015003305A MX2015003305A (es) 2016-01-20
MX366179B true MX366179B (es) 2019-07-01

Family

ID=48045767

Family Applications (3)

Application Number Title Priority Date Filing Date
MX2015003305A MX366179B (es) 2012-09-14 2013-03-15 Variantes de enzima con propiedades de ester sintasa mejoradas.
MX2019007766A MX2019007766A (es) 2012-09-14 2015-03-13 Variantes de enzima con propiedades de ester sintasa mejoradas.
MX2022002057A MX2022002057A (es) 2012-09-14 2015-03-13 Variantes de enzima con propiedades de ester sintasa mejoradas.

Family Applications After (2)

Application Number Title Priority Date Filing Date
MX2019007766A MX2019007766A (es) 2012-09-14 2015-03-13 Variantes de enzima con propiedades de ester sintasa mejoradas.
MX2022002057A MX2022002057A (es) 2012-09-14 2015-03-13 Variantes de enzima con propiedades de ester sintasa mejoradas.

Country Status (11)

Country Link
US (4) US9879239B2 (es)
EP (2) EP3299455A1 (es)
JP (6) JP6401703B2 (es)
KR (4) KR102493733B1 (es)
CN (2) CN112899250A (es)
AU (3) AU2013316124C1 (es)
BR (1) BR112015005782A2 (es)
CA (2) CA2885041C (es)
ES (1) ES2647412T3 (es)
MX (3) MX366179B (es)
WO (1) WO2014042693A1 (es)

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MX379251B (es) * 2012-04-02 2025-03-11 Genomatica Inc Producción mejorada de derivados de ácidos grasos.
EP2882843A4 (en) 2012-08-10 2016-04-06 Opx Biotechnologies Inc MICRO-ORGANISMS AND METHOD FOR THE PRODUCTION OF FATTY ACIDS AND PRODUCTS DERIVED FROM FATTY ACIDS
AU2013316124C1 (en) 2012-09-14 2022-01-06 Genomatica, Inc. Enzyme variants with improved ester synthase properties
US20150057465A1 (en) 2013-03-15 2015-02-26 Opx Biotechnologies, Inc. Control of growth-induction-production phases
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
CN106795483A (zh) 2013-07-19 2017-05-31 嘉吉公司 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法
EP2993228B1 (en) * 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
WO2018144701A2 (en) 2017-02-02 2018-08-09 Cargill Incorporated Genetically modified cells that produce c6-c10 fatty acid derivatives
BR112019020784A2 (pt) * 2017-04-03 2020-04-28 Genomatica, Inc. variantes de tioesterase com atividade melhorada para a produção de derivados de ácidos graxos de cadeia média
WO2020047304A1 (en) 2018-08-31 2020-03-05 Genomatica, Inc. Xylr mutant for improved xylose utilization or improved co-utilization of glucose and xylose
EP3844269A4 (en) 2018-08-31 2022-07-27 Genomatica, Inc. PRODUCTION OF NON-NATIVE MONOUNSATURATED FATTY ACIDS IN BACTERIA
BR112022003705A2 (pt) * 2019-08-30 2022-05-24 Codexis Inc Lipase recombinante, composição, sequência de polinucleotídeo recombinante, vetor de expressão, composição farmacêutica para o tratamento de insuficiência pancreática, célula hospedeira, métodos para produzir uma lipase recombinante e para tratar e/ou prevenir os sintomas de insuficiência pancreática, e, uso
AU2021379745A1 (en) * 2020-11-13 2023-07-06 Berkeley Fermentation Science Inc. Genetically engineered yeast cells and methods of use thereof
US12649937B2 (en) 2021-03-29 2026-06-09 Genomatica, Inc. Production of non-native monounsaturated fatty acids in bacteria
WO2023178193A1 (en) 2022-03-16 2023-09-21 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof
CN121801858A (zh) * 2026-03-12 2026-04-07 中国科学院天津工业生物技术研究所 一种烯烃还原酶突变体及其在催化合成沙库巴曲中间体中的应用

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EP0528881A4 (en) 1990-04-24 1993-05-26 Stratagene Methods for phenotype creation from multiple gene populations
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
JPWO2004074476A1 (ja) * 2003-02-21 2006-06-01 株式会社カネカ 新規ベクター
CN102533440B (zh) 2004-07-16 2017-09-19 杜邦营养生物科学有限公司 用酶使油脱胶的方法
US7897369B2 (en) * 2007-08-27 2011-03-01 Regents Of The University Of Minnesota Isoprenoid wax ester synthases, isoprenoid acyl CoA-synthetases, and uses thereof
CA3038491A1 (en) * 2008-10-07 2010-04-15 REG Life Sciences, LLC Bacterial host cells engineered to express a carboxylic acid reductase and a thioesterase
EP3564345A1 (en) * 2008-12-23 2019-11-06 REG Life Sciences, LLC Methods and compositions related to thioesterase enzymes
CN102656273B (zh) * 2009-09-25 2016-05-25 Reg生命科学有限责任公司 脂肪酸衍生物的产生
JP2011103863A (ja) 2009-11-20 2011-06-02 National Institute For Agro-Environmental Science デオキシニバレノールの分解活性を有するタンパク質をコードする遺伝子
US8530221B2 (en) * 2010-01-14 2013-09-10 Ls9, Inc. Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
ES2899431T3 (es) 2010-08-06 2022-03-11 Melt&Marble Ab Métodos y productos para la producción de ésteres de cera
WO2013048557A1 (en) * 2011-09-27 2013-04-04 Exxonmobil Research And Engineering Company Acyl-acp wax ester synthases
AU2013316124C1 (en) 2012-09-14 2022-01-06 Genomatica, Inc. Enzyme variants with improved ester synthase properties

Also Published As

Publication number Publication date
JP6820888B2 (ja) 2021-01-27
AU2013316124B2 (en) 2019-05-02
MX2022002057A (es) 2022-03-17
EP3299455A1 (en) 2018-03-28
AU2013316124C1 (en) 2022-01-06
KR102072048B1 (ko) 2020-02-03
MX2015003305A (es) 2016-01-20
EP2895602B1 (en) 2017-09-20
KR102339617B1 (ko) 2021-12-15
JP2021003131A (ja) 2021-01-14
JP6401703B2 (ja) 2018-10-10
BR112015005782A2 (pt) 2017-08-08
JP2020072747A (ja) 2020-05-14
JP2022051839A (ja) 2022-04-01
JP7030145B2 (ja) 2022-03-04
EP2895602A1 (en) 2015-07-22
JP2015528308A (ja) 2015-09-28
KR20200011595A (ko) 2020-02-03
US12398383B2 (en) 2025-08-26
AU2022200123A1 (en) 2022-02-10
KR102493733B1 (ko) 2023-02-06
US20180327728A1 (en) 2018-11-15
CA2885041A1 (en) 2014-03-20
US10894953B2 (en) 2021-01-19
CN104955944B (zh) 2021-03-09
KR20230020007A (ko) 2023-02-09
KR20150056603A (ko) 2015-05-26
CA2885041C (en) 2020-07-07
ES2647412T3 (es) 2017-12-21
JP7068362B2 (ja) 2022-05-16
AU2019202572A1 (en) 2019-05-02
JP2020072748A (ja) 2020-05-14
US20250101392A1 (en) 2025-03-27
KR20210153158A (ko) 2021-12-16
JP2019010112A (ja) 2019-01-24
AU2013316124A1 (en) 2015-04-02
CA3079799A1 (en) 2014-03-20
AU2019202572B2 (en) 2021-11-11
MX2019007766A (es) 2019-10-15
US9879239B2 (en) 2018-01-30
CN104955944A (zh) 2015-09-30
CN112899250A (zh) 2021-06-04
US20150299679A1 (en) 2015-10-22
US20210309979A1 (en) 2021-10-07
WO2014042693A1 (en) 2014-03-20

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