MX366179B - Variantes de enzima con propiedades de ester sintasa mejoradas. - Google Patents

Variantes de enzima con propiedades de ester sintasa mejoradas.

Info

Publication number
MX366179B
MX366179B MX2015003305A MX2015003305A MX366179B MX 366179 B MX366179 B MX 366179B MX 2015003305 A MX2015003305 A MX 2015003305A MX 2015003305 A MX2015003305 A MX 2015003305A MX 366179 B MX366179 B MX 366179B
Authority
MX
Mexico
Prior art keywords
enzyme variants
ester synthase
host cells
improved ester
recombinant host
Prior art date
Application number
MX2015003305A
Other languages
English (en)
Other versions
MX2015003305A (es
Inventor
M Da Costa Bernardo
holden Kevin
Helman Noah
Shumaker Andrew
G Hom Louis
S Baron Tarah
Original Assignee
Reg Life Sciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Reg Life Sciences Llc filed Critical Reg Life Sciences Llc
Publication of MX2015003305A publication Critical patent/MX2015003305A/es
Publication of MX366179B publication Critical patent/MX366179B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)
    • C12Y201/01015—Fatty-acid O-methyltransferase (2.1.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01067—Fatty-acyl-ethyl-ester synthase (3.1.1.67)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P30/00—Technologies relating to oil refining and petrochemical industry
    • Y02P30/20—Technologies relating to oil refining and petrochemical industry using bio-feedstock

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a variantes de enzima con propiedades de éster sintasa mejoradas para la producción de ésteres de ácido graso. Además se contemplan células hospederas recombinantes que expresan tales variantes, cultivos celulares que comprenden las células hospederas recombinantes y composiciones de éster de ácido graso producido por tales células hospederas recombinantes.
MX2015003305A 2012-09-14 2013-03-15 Variantes de enzima con propiedades de ester sintasa mejoradas. MX366179B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261701191P 2012-09-14 2012-09-14
US201261708424P 2012-10-01 2012-10-01
PCT/US2013/032564 WO2014042693A1 (en) 2012-09-14 2013-03-15 Enzyme variants with improved ester synthase properties

Publications (2)

Publication Number Publication Date
MX2015003305A MX2015003305A (es) 2016-01-20
MX366179B true MX366179B (es) 2019-07-01

Family

ID=48045767

Family Applications (3)

Application Number Title Priority Date Filing Date
MX2015003305A MX366179B (es) 2012-09-14 2013-03-15 Variantes de enzima con propiedades de ester sintasa mejoradas.
MX2022002057A MX2022002057A (es) 2012-09-14 2015-03-13 Variantes de enzima con propiedades de ester sintasa mejoradas.
MX2019007766A MX2019007766A (es) 2012-09-14 2015-03-13 Variantes de enzima con propiedades de ester sintasa mejoradas.

Family Applications After (2)

Application Number Title Priority Date Filing Date
MX2022002057A MX2022002057A (es) 2012-09-14 2015-03-13 Variantes de enzima con propiedades de ester sintasa mejoradas.
MX2019007766A MX2019007766A (es) 2012-09-14 2015-03-13 Variantes de enzima con propiedades de ester sintasa mejoradas.

Country Status (11)

Country Link
US (4) US9879239B2 (es)
EP (2) EP2895602B1 (es)
JP (6) JP6401703B2 (es)
KR (4) KR102493733B1 (es)
CN (2) CN104955944B (es)
AU (3) AU2013316124C1 (es)
BR (1) BR112015005782A2 (es)
CA (2) CA2885041C (es)
ES (1) ES2647412T3 (es)
MX (3) MX366179B (es)
WO (1) WO2014042693A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2834352B1 (en) * 2012-04-02 2020-01-08 Genomatica, Inc. Improved production of fatty acid derivatives
MX2015001780A (es) 2012-08-10 2015-09-28 Opx Biotechnologies Inc Microorganismos y métodos para la producción de ácidos grasos y productos derivados de ácidos grasos.
ES2647412T3 (es) 2012-09-14 2017-12-21 REG Life Sciences, LLC Variantes enzimáticas con propiedades de éster sintasa mejoradas
US20150119601A1 (en) 2013-03-15 2015-04-30 Opx Biotechnologies, Inc. Monofunctional mcr + 3-hp dehydrogenase
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
EP3022310B1 (en) 2013-07-19 2019-10-16 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
US11345938B2 (en) 2017-02-02 2022-05-31 Cargill, Incorporated Genetically modified cells that produce C6-C10 fatty acid derivatives
MY205555A (en) * 2017-04-03 2024-10-25 Genomatica Inc Thioesterase variants having improved activity for the production of medium-chain fatty acid derivatives
EP3844179A4 (en) 2018-08-31 2022-08-31 Genomatica, Inc. XYLR MUTANT FOR IMPROVED XYLOSE UTILIZATION OR IMPROVED CO-UTILIZATION OF GLUCOSE AND XYLOSE
US12221644B2 (en) 2018-08-31 2025-02-11 Genomatica, Inc. Production of non-native monounsaturated fatty acids in bacteria
EP4501403A3 (en) 2019-08-30 2025-05-14 Société des Produits Nestlé S.A. Engineered lipase variants
EP4244329A1 (en) * 2020-11-13 2023-09-20 Berkeley Fermentation Science Inc. Genetically engineered yeast cells and methods of use thereof
EP4493683A1 (en) 2022-03-16 2025-01-22 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016427A1 (en) 1990-04-24 1991-10-31 Stratagene Methods for phenotype creation from multiple gene populations
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
BRPI0407555A (pt) 2003-02-21 2006-02-14 Kaneka Corp vetor inédito
CN101052702B (zh) * 2004-07-16 2013-01-09 杜邦营养生物科学有限公司 脂肪分解酶及其在食品工业中的应用
US7897369B2 (en) 2007-08-27 2011-03-01 Regents Of The University Of Minnesota Isoprenoid wax ester synthases, isoprenoid acyl CoA-synthetases, and uses thereof
KR101717569B1 (ko) * 2008-10-07 2017-03-17 알이지 라이프 사이언시스, 엘엘씨 지방족 알데히드를 생산하기 위한 방법과 조성물들
CA3055144C (en) 2008-12-23 2022-07-19 REG Life Sciences, LLC Methods and compositions related to thioesterase enzymes
CA3056664A1 (en) * 2009-09-25 2011-03-31 Alfred Gaertner Production of fatty acid derivatives in recombinant bacterial cells expressing an ester synthase variant
JP2011103863A (ja) 2009-11-20 2011-06-02 National Institute For Agro-Environmental Science デオキシニバレノールの分解活性を有するタンパク質をコードする遺伝子
US8530221B2 (en) * 2010-01-14 2013-09-10 Ls9, Inc. Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
DK3382015T3 (da) * 2010-08-06 2021-11-22 Melt&Marble Ab Fremgangsmåder og produkter til fremstilling af voksestere
BR112014007115A2 (pt) * 2011-09-27 2017-04-25 Exxonmobil Res & Eng Co éster sintases de cera acil-acp
ES2647412T3 (es) 2012-09-14 2017-12-21 REG Life Sciences, LLC Variantes enzimáticas con propiedades de éster sintasa mejoradas

Also Published As

Publication number Publication date
AU2013316124C1 (en) 2022-01-06
JP2021003131A (ja) 2021-01-14
US20210309979A1 (en) 2021-10-07
AU2013316124B2 (en) 2019-05-02
JP2020072748A (ja) 2020-05-14
CA3079799A1 (en) 2014-03-20
AU2013316124A1 (en) 2015-04-02
KR102072048B1 (ko) 2020-02-03
ES2647412T3 (es) 2017-12-21
AU2019202572A1 (en) 2019-05-02
EP3299455A1 (en) 2018-03-28
MX2015003305A (es) 2016-01-20
JP2015528308A (ja) 2015-09-28
AU2019202572B2 (en) 2021-11-11
MX2022002057A (es) 2022-03-17
BR112015005782A2 (pt) 2017-08-08
CA2885041A1 (en) 2014-03-20
JP6820888B2 (ja) 2021-01-27
CN104955944B (zh) 2021-03-09
US12398383B2 (en) 2025-08-26
JP2022051839A (ja) 2022-04-01
KR102493733B1 (ko) 2023-02-06
US20180327728A1 (en) 2018-11-15
CN112899250A (zh) 2021-06-04
CN104955944A (zh) 2015-09-30
WO2014042693A1 (en) 2014-03-20
JP2019010112A (ja) 2019-01-24
US20250101392A1 (en) 2025-03-27
MX2019007766A (es) 2019-10-15
US9879239B2 (en) 2018-01-30
KR20230020007A (ko) 2023-02-09
KR102339617B1 (ko) 2021-12-15
JP7068362B2 (ja) 2022-05-16
EP2895602A1 (en) 2015-07-22
KR20150056603A (ko) 2015-05-26
EP2895602B1 (en) 2017-09-20
JP7030145B2 (ja) 2022-03-04
JP6401703B2 (ja) 2018-10-10
US20150299679A1 (en) 2015-10-22
JP2020072747A (ja) 2020-05-14
KR20200011595A (ko) 2020-02-03
CA2885041C (en) 2020-07-07
AU2022200123A1 (en) 2022-02-10
KR20210153158A (ko) 2021-12-16
US10894953B2 (en) 2021-01-19

Similar Documents

Publication Publication Date Title
MX366179B (es) Variantes de enzima con propiedades de ester sintasa mejoradas.
MY181688A (en) Improved production of fatty acid derivatives
MX358177B (es) Enzimas de acido carboxilico-reductasa (car) y produccion mejorada de alcoholes grasos.
PH12014502795A1 (en) Production of long chain polyunsaturated fatty acids in plant cells
WO2014093505A3 (en) Acp-mediated production of fatty acid derivatives
IN2014CN02100A (es)
WO2011127409A3 (en) Methods and compositions related to fatty alcohol biosynthetic enzymes
ZA201707452B (en) Cell culture compositions and methods for polypeptide production
MX2014012810A (es) Sistemas de celulas y metodos para mejorar la sintesis de acidos grasos mediante la expresion de deshidrogenasas.
MX346518B (es) Metodos de desarrollo de variantes de terpeno sintasa.
MX2013004353A (es) Produccion recombinante de n-propanol e isopropanol.
WO2011156520A3 (en) Compositions and methods for increasing oil production and secretion
MX356997B (es) Acil-acp reductasa con propiedades mejoradas.
MY180645A (en) Microbial production of fatty amines
EP3456814A3 (en) Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
ZA201409404B (en) Production of enzymes for ligno-cellulosic biomass
MX2014007760A (es) Bacterias con unidades transcripcionales reconstruidas y los usos de las mismas.
MY176277A (en) Improved acetyl-coa carboxylase variants

Legal Events

Date Code Title Description
FG Grant or registration