MX366179B - Variantes de enzima con propiedades de ester sintasa mejoradas. - Google Patents

Variantes de enzima con propiedades de ester sintasa mejoradas.

Info

Publication number
MX366179B
MX366179B MX2015003305A MX2015003305A MX366179B MX 366179 B MX366179 B MX 366179B MX 2015003305 A MX2015003305 A MX 2015003305A MX 2015003305 A MX2015003305 A MX 2015003305A MX 366179 B MX366179 B MX 366179B
Authority
MX
Mexico
Prior art keywords
enzyme variants
ester synthase
host cells
improved ester
recombinant host
Prior art date
Application number
MX2015003305A
Other languages
English (en)
Other versions
MX2015003305A (es
Inventor
M Da Costa Bernardo
holden Kevin
Helman Noah
Shumaker Andrew
G Hom Louis
S Baron Tarah
Original Assignee
Reg Life Sciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Reg Life Sciences Llc filed Critical Reg Life Sciences Llc
Publication of MX2015003305A publication Critical patent/MX2015003305A/es
Publication of MX366179B publication Critical patent/MX366179B/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)
    • C12Y201/01015—Fatty-acid O-methyltransferase (2.1.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01067—Fatty-acyl-ethyl-ester synthase (3.1.1.67)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P30/00—Technologies relating to oil refining and petrochemical industry
    • Y02P30/20—Technologies relating to oil refining and petrochemical industry using bio-feedstock

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a variantes de enzima con propiedades de éster sintasa mejoradas para la producción de ésteres de ácido graso. Además se contemplan células hospederas recombinantes que expresan tales variantes, cultivos celulares que comprenden las células hospederas recombinantes y composiciones de éster de ácido graso producido por tales células hospederas recombinantes.
MX2015003305A 2012-09-14 2013-03-15 Variantes de enzima con propiedades de ester sintasa mejoradas. MX366179B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261701191P 2012-09-14 2012-09-14
US201261708424P 2012-10-01 2012-10-01
PCT/US2013/032564 WO2014042693A1 (en) 2012-09-14 2013-03-15 Enzyme variants with improved ester synthase properties

Publications (2)

Publication Number Publication Date
MX2015003305A MX2015003305A (es) 2016-01-20
MX366179B true MX366179B (es) 2019-07-01

Family

ID=48045767

Family Applications (3)

Application Number Title Priority Date Filing Date
MX2015003305A MX366179B (es) 2012-09-14 2013-03-15 Variantes de enzima con propiedades de ester sintasa mejoradas.
MX2022002057A MX2022002057A (es) 2012-09-14 2015-03-13 Variantes de enzima con propiedades de ester sintasa mejoradas.
MX2019007766A MX2019007766A (es) 2012-09-14 2015-03-13 Variantes de enzima con propiedades de ester sintasa mejoradas.

Family Applications After (2)

Application Number Title Priority Date Filing Date
MX2022002057A MX2022002057A (es) 2012-09-14 2015-03-13 Variantes de enzima con propiedades de ester sintasa mejoradas.
MX2019007766A MX2019007766A (es) 2012-09-14 2015-03-13 Variantes de enzima con propiedades de ester sintasa mejoradas.

Country Status (11)

Country Link
US (4) US9879239B2 (es)
EP (2) EP3299455A1 (es)
JP (6) JP6401703B2 (es)
KR (4) KR102072048B1 (es)
CN (2) CN104955944B (es)
AU (3) AU2013316124C1 (es)
BR (1) BR112015005782A2 (es)
CA (2) CA2885041C (es)
ES (1) ES2647412T3 (es)
MX (3) MX366179B (es)
WO (1) WO2014042693A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112014024675B1 (pt) * 2012-04-02 2021-12-07 Genomatica, Inc Célula hospedeira recombinante
KR20150040359A (ko) 2012-08-10 2015-04-14 오피엑스 바이오테크놀로지스, 인크. 지방산 및 지방산 유도된 산물의 생산을 위한 미생물 및 방법
US9879239B2 (en) 2012-09-14 2018-01-30 REG Life Sciences, LLC Enzyme variants with improved ester synthase properties
US20150057465A1 (en) 2013-03-15 2015-02-26 Opx Biotechnologies, Inc. Control of growth-induction-production phases
US10337038B2 (en) 2013-07-19 2019-07-02 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
EP3577227A4 (en) 2017-02-02 2020-12-30 Cargill Inc. GENETICALLY MODIFIED CELLS PRODUCING C6-C10 FATTY ACID DERIVATIVES
JP7705243B2 (ja) * 2017-04-03 2025-07-09 ジェノマティカ， インコーポレイテッド 中鎖脂肪酸誘導体の生産のための改良された活性を有するチオエステラーゼ変種
CN112673016B (zh) 2018-08-31 2025-08-05 基因组股份公司 用于改善的木糖利用或改善的葡萄糖和木糖共利用的xylr突变体
US12221644B2 (en) 2018-08-31 2025-02-11 Genomatica, Inc. Production of non-native monounsaturated fatty acids in bacteria
MX2022002473A (es) * 2019-08-30 2022-08-02 Codexis Inc Variantes de lipasa modificadas geneticamente.
JP2023549539A (ja) * 2020-11-13 2023-11-27 バークレー ファーメンテイション サイエンス インコーポレーテッド 遺伝子学的に改変された酵母細胞およびこれの使用の方法
EP4493683A1 (en) 2022-03-16 2025-01-22 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof

Family Cites Families (14)

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AU7791991A (en) 1990-04-24 1991-11-11 Stratagene Methods for phenotype creation from multiple gene populations
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
JPWO2004074476A1 (ja) 2003-02-21 2006-06-01 株式会社カネカ 新規ベクター
EP1776455B1 (en) 2004-07-16 2015-03-18 DuPont Nutrition Biosciences ApS Lipolytic enzyme, uses thereof in the food industry
US7897369B2 (en) * 2007-08-27 2011-03-01 Regents Of The University Of Minnesota Isoprenoid wax ester synthases, isoprenoid acyl CoA-synthetases, and uses thereof
EP2352824B1 (en) * 2008-10-07 2015-09-02 REG Life Sciences, LLC Method and compositions for producing fatty aldehydes
EP2382285B1 (en) * 2008-12-23 2019-05-01 REG Life Sciences, LLC Methods and compositions related to thioesterase enzymes
ES2536240T3 (es) * 2009-09-25 2015-05-21 REG Life Sciences, LLC Producción de derivados de ácidos grasos
JP2011103863A (ja) * 2009-11-20 2011-06-02 National Institute For Agro-Environmental Science デオキシニバレノールの分解活性を有するタンパク質をコードする遺伝子
US8530221B2 (en) * 2010-01-14 2013-09-10 Ls9, Inc. Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
EP2601292B1 (en) * 2010-08-06 2018-04-18 Biopetrolia AB Methods and products for production of wax esters
EP2753701B1 (en) * 2011-09-27 2018-05-02 ExxonMobil Research and Engineering Company Acyl-acp wax ester synthases
US9879239B2 (en) 2012-09-14 2018-01-30 REG Life Sciences, LLC Enzyme variants with improved ester synthase properties

Also Published As

Publication number Publication date
JP2019010112A (ja) 2019-01-24
CA3079799A1 (en) 2014-03-20
MX2019007766A (es) 2019-10-15
EP2895602A1 (en) 2015-07-22
JP2022051839A (ja) 2022-04-01
CN112899250A (zh) 2021-06-04
KR20210153158A (ko) 2021-12-16
US20250101392A1 (en) 2025-03-27
EP3299455A1 (en) 2018-03-28
JP6820888B2 (ja) 2021-01-27
JP6401703B2 (ja) 2018-10-10
JP2021003131A (ja) 2021-01-14
US12398383B2 (en) 2025-08-26
US20150299679A1 (en) 2015-10-22
JP7068362B2 (ja) 2022-05-16
MX2015003305A (es) 2016-01-20
KR20230020007A (ko) 2023-02-09
KR102493733B1 (ko) 2023-02-06
CN104955944B (zh) 2021-03-09
CA2885041C (en) 2020-07-07
JP2020072748A (ja) 2020-05-14
US10894953B2 (en) 2021-01-19
WO2014042693A1 (en) 2014-03-20
BR112015005782A2 (pt) 2017-08-08
JP7030145B2 (ja) 2022-03-04
MX2022002057A (es) 2022-03-17
US20180327728A1 (en) 2018-11-15
AU2019202572B2 (en) 2021-11-11
AU2013316124A1 (en) 2015-04-02
US9879239B2 (en) 2018-01-30
AU2022200123A1 (en) 2022-02-10
KR102339617B1 (ko) 2021-12-15
AU2013316124C1 (en) 2022-01-06
KR20200011595A (ko) 2020-02-03
CN104955944A (zh) 2015-09-30
CA2885041A1 (en) 2014-03-20
AU2019202572A1 (en) 2019-05-02
EP2895602B1 (en) 2017-09-20
ES2647412T3 (es) 2017-12-21
KR20150056603A (ko) 2015-05-26
AU2013316124B2 (en) 2019-05-02
KR102072048B1 (ko) 2020-02-03
JP2020072747A (ja) 2020-05-14
JP2015528308A (ja) 2015-09-28
US20210309979A1 (en) 2021-10-07

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