MX337128B - Sistemas de celulas y metodos para mejorar la sintesis de acidos grasos mediante la expresion de deshidrogenasas. - Google Patents

Sistemas de celulas y metodos para mejorar la sintesis de acidos grasos mediante la expresion de deshidrogenasas.

Info

Publication number
MX337128B
MX337128B MX2014012810A MX2014012810A MX337128B MX 337128 B MX337128 B MX 337128B MX 2014012810 A MX2014012810 A MX 2014012810A MX 2014012810 A MX2014012810 A MX 2014012810A MX 337128 B MX337128 B MX 337128B
Authority
MX
Mexico
Prior art keywords
methods
fatty acid
dehydrogenases
expression
microorganisms
Prior art date
Application number
MX2014012810A
Other languages
English (en)
Other versions
MX2014012810A (es
Inventor
Srividya Akella
Robert C Brown
Jennifer Coppersmith
Prachee Prakash
Rekha Seshadri
Original Assignee
Exxonmobil Res & Eng Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exxonmobil Res & Eng Co filed Critical Exxonmobil Res & Eng Co
Publication of MX2014012810A publication Critical patent/MX2014012810A/es
Publication of MX337128B publication Critical patent/MX337128B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01044—Phosphogluconate dehydrogenase (decarboxylating) (1.1.1.44)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/99—Oxidoreductases acting on the CH-OH group of donors (1.1) with other acceptors (1.1.99)
    • C12Y101/99006—D-2-Hydroxy-acid dehydrogenase (1.1.99.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)

Abstract

La presente invención se refiere a métodos para producir lípidos tales como productos de ácidos grasos en células hospederas recombinantes diseñadas para expresar un gen no nativo que codifica para una deshidrogenasa; los microorganismos recombinantes son capaces de proliferar a una mayor tasa en comparación con los microorganismos que no expresan un gen de deshidrogenasa no nativo, y los cultivos de microorganismos que son diseñados para la producción de lípidos y que expresan un gen de deshidrogenasa no nativo producen más lípido que los cultivos de microorganismos control que no incluyen un gen de deshidrogenasa no nativo.
MX2014012810A 2012-04-23 2012-12-05 Sistemas de celulas y metodos para mejorar la sintesis de acidos grasos mediante la expresion de deshidrogenasas. MX337128B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/453,235 US9181568B2 (en) 2012-04-23 2012-04-23 Cell systems and methods for improving fatty acid synthesis by expression of dehydrogenases
PCT/US2012/067901 WO2013162648A1 (en) 2012-04-23 2012-12-05 Cell systems and methods for improving fatty acid synthesis by expression of dehydrogenases

Publications (2)

Publication Number Publication Date
MX2014012810A MX2014012810A (es) 2015-01-22
MX337128B true MX337128B (es) 2016-02-12

Family

ID=49380459

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014012810A MX337128B (es) 2012-04-23 2012-12-05 Sistemas de celulas y metodos para mejorar la sintesis de acidos grasos mediante la expresion de deshidrogenasas.

Country Status (13)

Country Link
US (1) US9181568B2 (es)
EP (1) EP2841585B1 (es)
JP (2) JP6209590B2 (es)
CN (1) CN104126013B (es)
AU (1) AU2012378148B2 (es)
BR (1) BR112014026206A2 (es)
CA (1) CA2871373C (es)
IL (1) IL234810A (es)
MX (1) MX337128B (es)
MY (1) MY172288A (es)
SG (1) SG11201406546UA (es)
WO (1) WO2013162648A1 (es)
ZA (1) ZA201407306B (es)

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Also Published As

Publication number Publication date
MX2014012810A (es) 2015-01-22
JP2017123852A (ja) 2017-07-20
EP2841585B1 (en) 2020-03-25
JP2015517811A (ja) 2015-06-25
AU2012378148B2 (en) 2016-11-24
US20130280793A1 (en) 2013-10-24
CA2871373A1 (en) 2013-10-31
EP2841585A1 (en) 2015-03-04
SG11201406546UA (en) 2014-11-27
IL234810A (en) 2016-09-29
MY172288A (en) 2019-11-20
CN104126013A (zh) 2014-10-29
CN104126013B (zh) 2019-05-14
AU2012378148A1 (en) 2014-11-13
ZA201407306B (en) 2015-11-25
JP6209590B2 (ja) 2017-10-04
US9181568B2 (en) 2015-11-10
WO2013162648A1 (en) 2013-10-31
BR112014026206A2 (pt) 2017-07-18
EP2841585A4 (en) 2016-09-28
CA2871373C (en) 2016-07-19

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