AR086031A2 - Un metodo para remover al menos una secuencia transgenica en una celula vegetal o planta - Google Patents

Un metodo para remover al menos una secuencia transgenica en una celula vegetal o planta

Info

Publication number
AR086031A2
AR086031A2 ARP120101393A ARP120101393A AR086031A2 AR 086031 A2 AR086031 A2 AR 086031A2 AR P120101393 A ARP120101393 A AR P120101393A AR P120101393 A ARP120101393 A AR P120101393A AR 086031 A2 AR086031 A2 AR 086031A2
Authority
AR
Argentina
Prior art keywords
sequence
homology
transgenic
plant
double
Prior art date
Application number
ARP120101393A
Other languages
English (en)
Inventor
Puchta Holger Dr
Biesgen Christian Dr
Original Assignee
Sungene Gmbh & Co Kgaa
Inst F Pflanzengenetik U Kulturpflanzenforschung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sungene Gmbh & Co Kgaa, Inst F Pflanzengenetik U Kulturpflanzenforschung filed Critical Sungene Gmbh & Co Kgaa
Publication of AR086031A2 publication Critical patent/AR086031A2/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

Un método para remover al menos una secuencia transgénica en una célula vegetal o planta, donde dicha célula vegetal o planta contiene insertado en el ADN cromosomal un constructo de recombinación transgénico comprendiendo una secuencia que se compone en dirección 5’/3’ de: i) una primera secuencia de homología A, y ii) por lo menos una secuencia transgénica a ser removida, y iii) una segunda secuencia de homología B, presentando las secuencias de homología A y B una longitud y una homología suficientes para asegurar una recombinación homóloga entre ambas, donde el constructo de recombinación transgénica tiene al menos una secuencia de reconocimiento para la inducción especifica de rotura ADN bicatenario, localizado entre la secuencia de homología A y la secuencia de homología B, donde se agrega una enzima endonucleasa a dicha célula vegetal o planta para la inducción de roturas de ADN bicatenario o una secuencia de ácido nucleico que codifica dicha enzima endonucleasa apropiada para la inducción de roturas de ADN bicatenario en la secuenciada reconocimiento de (ii), donde todas las secuencias de reconocimiento para la inducción especifica de roturas de ADN bicatenario para dicha enzima están localizadas entre la secuencia de homología A y la secuencia de homología B y están localizadas en uno de 5’, 3’ o dentro de por lo menos una secuencia transgénica a ser removida y donde después de la recombinación homóloga de las secuencias de homología A y B la resultante secuencia transgénica derivada de dicho constructo de recombinación transgénico no comprende ningún sitio de reconocimiento específico para dicha enzima endonucleasa adecuada para inducción de roturas de ADN bicatenario.
ARP120101393A 2001-07-04 2012-04-23 Un metodo para remover al menos una secuencia transgenica en una celula vegetal o planta AR086031A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10131786A DE10131786A1 (de) 2001-07-04 2001-07-04 Rekombinationssysteme und Verfahren zum Entfernen von Nukleinsäuresequenzen aus dem Genom eukaryotischer Organismen

Publications (1)

Publication Number Publication Date
AR086031A2 true AR086031A2 (es) 2013-11-13

Family

ID=7690173

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP020102493A AR034687A1 (es) 2001-07-04 2002-07-03 Sistemas de recombinacion y procedimiento para eliminar secuencias de acido nucleinico a partir del genoma de organismos eucariotas
ARP120101393A AR086031A2 (es) 2001-07-04 2012-04-23 Un metodo para remover al menos una secuencia transgenica en una celula vegetal o planta

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP020102493A AR034687A1 (es) 2001-07-04 2002-07-03 Sistemas de recombinacion y procedimiento para eliminar secuencias de acido nucleinico a partir del genoma de organismos eucariotas

Country Status (12)

Country Link
US (1) US7736886B2 (es)
EP (1) EP1407034B1 (es)
JP (1) JP2004533267A (es)
CN (1) CN100451123C (es)
AR (2) AR034687A1 (es)
AU (1) AU2002325859B2 (es)
BR (2) BR0210839A (es)
CA (1) CA2451492C (es)
DE (1) DE10131786A1 (es)
IL (1) IL159286A0 (es)
MX (1) MXPA03011844A (es)
WO (1) WO2003004659A2 (es)

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Also Published As

Publication number Publication date
US20050172365A1 (en) 2005-08-04
WO2003004659A2 (de) 2003-01-16
WO2003004659A8 (de) 2004-04-22
WO2003004659A3 (de) 2003-09-25
EP1407034B1 (de) 2016-09-07
AR034687A1 (es) 2004-03-03
US7736886B2 (en) 2010-06-15
CA2451492C (en) 2014-12-16
DE10131786A1 (de) 2003-01-16
BRPI0210839B1 (pt) 2019-02-26
MXPA03011844A (es) 2005-03-07
CA2451492A1 (en) 2003-01-16
EP1407034A2 (de) 2004-04-14
IL159286A0 (en) 2004-06-01
AU2002325859B2 (en) 2008-05-29
JP2004533267A (ja) 2004-11-04
CN1628174A (zh) 2005-06-15
CN100451123C (zh) 2009-01-14
BR0210839A (pt) 2004-07-13

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