AR086031A2 - Un metodo para remover al menos una secuencia transgenica en una celula vegetal o planta - Google Patents
Un metodo para remover al menos una secuencia transgenica en una celula vegetal o plantaInfo
- Publication number
- AR086031A2 AR086031A2 ARP120101393A ARP120101393A AR086031A2 AR 086031 A2 AR086031 A2 AR 086031A2 AR P120101393 A ARP120101393 A AR P120101393A AR P120101393 A ARP120101393 A AR P120101393A AR 086031 A2 AR086031 A2 AR 086031A2
- Authority
- AR
- Argentina
- Prior art keywords
- sequence
- homology
- transgenic
- plant
- double
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Saccharide Compounds (AREA)
Abstract
Un método para remover al menos una secuencia transgénica en una célula vegetal o planta, donde dicha célula vegetal o planta contiene insertado en el ADN cromosomal un constructo de recombinación transgénico comprendiendo una secuencia que se compone en dirección 5’/3’ de: i) una primera secuencia de homología A, y ii) por lo menos una secuencia transgénica a ser removida, y iii) una segunda secuencia de homología B, presentando las secuencias de homología A y B una longitud y una homología suficientes para asegurar una recombinación homóloga entre ambas, donde el constructo de recombinación transgénica tiene al menos una secuencia de reconocimiento para la inducción especifica de rotura ADN bicatenario, localizado entre la secuencia de homología A y la secuencia de homología B, donde se agrega una enzima endonucleasa a dicha célula vegetal o planta para la inducción de roturas de ADN bicatenario o una secuencia de ácido nucleico que codifica dicha enzima endonucleasa apropiada para la inducción de roturas de ADN bicatenario en la secuenciada reconocimiento de (ii), donde todas las secuencias de reconocimiento para la inducción especifica de roturas de ADN bicatenario para dicha enzima están localizadas entre la secuencia de homología A y la secuencia de homología B y están localizadas en uno de 5’, 3’ o dentro de por lo menos una secuencia transgénica a ser removida y donde después de la recombinación homóloga de las secuencias de homología A y B la resultante secuencia transgénica derivada de dicho constructo de recombinación transgénico no comprende ningún sitio de reconocimiento específico para dicha enzima endonucleasa adecuada para inducción de roturas de ADN bicatenario.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10131786A DE10131786A1 (de) | 2001-07-04 | 2001-07-04 | Rekombinationssysteme und Verfahren zum Entfernen von Nukleinsäuresequenzen aus dem Genom eukaryotischer Organismen |
Publications (1)
Publication Number | Publication Date |
---|---|
AR086031A2 true AR086031A2 (es) | 2013-11-13 |
Family
ID=7690173
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP020102493A AR034687A1 (es) | 2001-07-04 | 2002-07-03 | Sistemas de recombinacion y procedimiento para eliminar secuencias de acido nucleinico a partir del genoma de organismos eucariotas |
ARP120101393A AR086031A2 (es) | 2001-07-04 | 2012-04-23 | Un metodo para remover al menos una secuencia transgenica en una celula vegetal o planta |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP020102493A AR034687A1 (es) | 2001-07-04 | 2002-07-03 | Sistemas de recombinacion y procedimiento para eliminar secuencias de acido nucleinico a partir del genoma de organismos eucariotas |
Country Status (12)
Country | Link |
---|---|
US (1) | US7736886B2 (es) |
EP (1) | EP1407034B1 (es) |
JP (1) | JP2004533267A (es) |
CN (1) | CN100451123C (es) |
AR (2) | AR034687A1 (es) |
AU (1) | AU2002325859B2 (es) |
BR (2) | BR0210839A (es) |
CA (1) | CA2451492C (es) |
DE (1) | DE10131786A1 (es) |
IL (1) | IL159286A0 (es) |
MX (1) | MXPA03011844A (es) |
WO (1) | WO2003004659A2 (es) |
Families Citing this family (75)
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US7102055B1 (en) * | 1997-11-18 | 2006-09-05 | Pioneer Hi-Bred International, Inc. | Compositions and methods for the targeted insertion of a nucleotide sequence of interest into the genome of a plant |
AU757672B2 (en) * | 1997-11-18 | 2003-02-27 | Pioneer Hi-Bred International, Inc. | A method for directional stable transformation of eukaryotic cells |
WO2003078619A1 (en) * | 2002-03-15 | 2003-09-25 | Cellectis | Hybrid and single chain meganucleases and use thereof |
WO2009095742A1 (en) * | 2008-01-31 | 2009-08-06 | Cellectis | New i-crei derived single-chain meganuclease and uses thereof |
US20100151556A1 (en) * | 2002-03-15 | 2010-06-17 | Cellectis | Hybrid and single chain meganucleases and use thereof |
CA2493364A1 (en) | 2002-07-26 | 2004-02-12 | Basf Plant Science Gmbh | Inversion of the negative-selective effect of negative marker proteins using selection methods |
JP2006518372A (ja) | 2003-01-28 | 2006-08-10 | セレクティス | 脊椎動物の体組織においてエクスビボかつイントトで相同的組換えを誘発するためのメガヌクレアーゼの使用およびその応用 |
US7476729B2 (en) * | 2003-10-24 | 2009-01-13 | Institut Curie | Dbait and uses thereof |
WO2005049842A2 (en) | 2003-11-18 | 2005-06-02 | Bayer Bioscience N.V. | Improved targeted dna insertion in plants |
WO2005090581A1 (en) * | 2004-03-17 | 2005-09-29 | Basf Plant Science Gmbh | Improved constructs for marker excision based on dual-function selection marker |
WO2005095624A2 (en) | 2004-04-02 | 2005-10-13 | Dsm Ip Assets B.V. | Filamentous fungal mutants with improved homologous recombination efficiency |
CA2578140A1 (en) * | 2004-09-23 | 2006-03-30 | Basf Plant Science Gmbh | Recombination cassettes and methods for sequence excision in plants |
EP1669456A3 (en) | 2004-12-11 | 2006-07-12 | SunGene GmbH | Expression cassettes for meristem-preferential expression in plants |
WO2006074956A1 (en) * | 2005-01-14 | 2006-07-20 | Bayer Bioscience N.V. | Improved plant transformation methods |
CN101155921B (zh) * | 2005-04-04 | 2013-02-06 | 拜尔作物科学公司 | 用于去除所选择dna序列的方法和手段 |
CN101198701B (zh) | 2005-04-19 | 2013-04-24 | 巴斯福植物科学有限公司 | 单子叶植物中的淀粉胚乳特异性和/或萌芽胚特异性表达 |
EP1907553B1 (en) * | 2005-07-18 | 2012-08-22 | Pioneer Hi-Bred International Inc. | Modified frt recombination sites and methods of use |
EP2484758B1 (en) | 2005-10-18 | 2013-10-02 | Precision Biosciences | Rationally-designed meganucleases with altered sequence specificity and DNA-binding affinity |
WO2007107516A2 (en) | 2006-03-17 | 2007-09-27 | Basf Plant Science Gmbh | D-amino acid selection for soybean |
EP2004827B1 (en) * | 2006-04-08 | 2010-03-10 | DSM IP Assets B.V. | Improved method for homologous recombination in fungal cells |
AU2007253458B2 (en) | 2006-05-18 | 2013-04-04 | Biogemma | Method for performing homologous recombination in plants |
WO2008037436A1 (en) | 2006-09-28 | 2008-04-03 | Bayer Bioscience N.V. | Methods and means for removal of a selected dna sequence |
WO2008099013A1 (en) | 2007-02-16 | 2008-08-21 | Basf Plant Science Gmbh | Nucleic acid sequences for regulation of embryo-specific expression in monocotyledonous plants |
CA2688246C (en) * | 2007-05-31 | 2018-06-19 | Basf Plant Science Gmbh | Method of excising a nucleic acid sequence from a plant genome |
BRPI0812233B1 (pt) | 2007-06-05 | 2022-10-04 | Bayer Cropscience Ag | Processos para troca de uma sequência de dna-alvo no genoma de uma célula de planta ou planta por uma sequência de dna de interesse, e vetor de dna |
EP2568048A1 (en) | 2007-06-29 | 2013-03-13 | Pioneer Hi-Bred International, Inc. | Methods for altering the genome of a monocot plant cell |
US9267132B2 (en) | 2007-10-08 | 2016-02-23 | Synthetic Genomics, Inc. | Methods for cloning and manipulating genomes |
CA2703079A1 (en) * | 2007-12-07 | 2009-06-18 | Precision Biosciences, Inc. | Rationally-designed meganucleases with recognition sequences found in dnase hypersensitive regions of the human genome |
WO2009114321A2 (en) | 2008-03-11 | 2009-09-17 | Precision Biosciencs, Inc. | Rationally-designed meganucleases for maize genome engineering |
US20100071083A1 (en) * | 2008-03-12 | 2010-03-18 | Smith James J | Temperature-dependent meganuclease activity |
AU2009241351A1 (en) * | 2008-04-28 | 2009-11-05 | Precision Biosciences, Inc. | Fusion molecules of rationally-designed DNA-binding proteins and effector domains |
JP2011527906A (ja) | 2008-07-14 | 2011-11-10 | プレシジョン バイオサイエンシズ,インク. | I−CreI由来メガヌクレアーゼの認識配列およびその使用 |
CN105274004B (zh) * | 2009-03-06 | 2019-08-09 | 合成基因组股份有限公司 | 用于克隆和操作基因组的方法 |
US20110269119A1 (en) | 2009-10-30 | 2011-11-03 | Synthetic Genomics, Inc. | Encoding text into nucleic acid sequences |
CA2787494C (en) | 2010-01-22 | 2019-09-17 | Dow Agrosciences Llc | Targeted genomic alteration |
KR101137819B1 (ko) | 2010-06-28 | 2012-06-27 | 대한민국 | 백합나무의 배발생조직 증식방법 |
SG187680A1 (en) * | 2010-08-02 | 2013-03-28 | Cellectis | Method for targeted genomic events in algae |
US9187762B2 (en) | 2010-08-13 | 2015-11-17 | Pioneer Hi-Bred International, Inc. | Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (HPPD) activity |
US7919605B1 (en) | 2010-08-30 | 2011-04-05 | Amyris, Inc. | Nucleic acids, compositions and methods for the excision of target nucleic acids |
JP5740955B2 (ja) * | 2010-12-10 | 2015-07-01 | 三菱レイヨン株式会社 | ロドコッカス属細菌の形質転換のためのランダム遺伝子導入用ツール |
CN102559704B (zh) * | 2010-12-23 | 2014-08-27 | 中国科学院上海生命科学研究院 | 一种在丙酮丁醇梭菌中敲除基因的方法 |
BR112013024337A2 (pt) | 2011-03-23 | 2017-09-26 | Du Pont | locus de traço transgênico complexo em uma planta, planta ou semente, método para produzir em uma planta um locus de traço transgênico complexo e construto de expressão |
MX348785B (es) | 2011-06-06 | 2017-06-28 | Bayer Cropscience Nv | Metodos y medios para modificar un genoma vegetal en un sitio preseleccionado. |
WO2013017853A2 (en) * | 2011-07-29 | 2013-02-07 | Cambridge Epigenetix Limited | Methods for detection of nucleotide modification |
MX346439B (es) | 2011-08-22 | 2017-03-17 | Bayer Cropscience Nv | Métodos y medios para modificar un genoma vegetal. |
BR112014016774A2 (pt) | 2012-01-06 | 2020-10-27 | Pioneer Hi-Bred International, Inc | método para produzir grande população de sementes, planta autorreprodutora e semente |
CN104245940A (zh) | 2012-04-23 | 2014-12-24 | 拜尔作物科学公司 | 植物中的靶向基因组工程 |
CN102703426A (zh) * | 2012-05-21 | 2012-10-03 | 吴江汇杰生物科技有限公司 | 构建核酸库的方法、试剂及试剂盒 |
UA119135C2 (uk) * | 2012-09-07 | 2019-05-10 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб отримання трансгенної рослини |
WO2014161821A1 (en) | 2013-04-02 | 2014-10-09 | Bayer Cropscience Nv | Targeted genome engineering in eukaryotes |
EP3611268A1 (en) | 2013-08-22 | 2020-02-19 | E. I. du Pont de Nemours and Company | Plant genome modification using guide rna/cas endonuclease systems and methods of use |
EP3362568A1 (en) | 2015-10-16 | 2018-08-22 | Pioneer Hi-Bred International, Inc. | Methods and compositions for transformation comprising negative selection markers |
US11021713B2 (en) | 2016-05-25 | 2021-06-01 | Cargill, Incorporated | Engineered nucleases to generate deletion mutants in plants |
WO2017202715A1 (en) | 2016-05-26 | 2017-11-30 | Nunhems B.V. | Seedless fruit producing plants |
US11359234B2 (en) * | 2016-07-01 | 2022-06-14 | Microsoft Technology Licensing, Llc | Barcoding sequences for identification of gene expression |
US20180004537A1 (en) | 2016-07-01 | 2018-01-04 | Microsoft Technology Licensing, Llc | Molecular State Machines |
CA3046929A1 (en) | 2016-12-14 | 2018-06-21 | Dow Agrosciences Llc | Reconstruction of site specific nuclease binding sites |
EP3501268B1 (en) | 2017-12-22 | 2021-09-15 | KWS SAAT SE & Co. KGaA | Regeneration of plants in the presence of histone deacetylase inhibitors |
EP3508581A1 (en) | 2018-01-03 | 2019-07-10 | Kws Saat Se | Regeneration of genetically modified plants |
CA3086620A1 (en) | 2018-01-12 | 2019-07-18 | Basf Se | Gene underlying the number of spikelets per spike qtl in wheat on chromosome 7a |
EP3545756A1 (en) | 2018-03-28 | 2019-10-02 | KWS SAAT SE & Co. KGaA | Regeneration of plants in the presence of inhibitors of the histone methyltransferase ezh2 |
EP3567111A1 (en) | 2018-05-09 | 2019-11-13 | KWS SAAT SE & Co. KGaA | Gene for resistance to a pathogen of the genus heterodera |
EP3807299A1 (en) | 2018-06-15 | 2021-04-21 | KWS SAAT SE & Co. KGaA | Methods for enhancing genome engineering efficiency |
BR112020025349A2 (pt) | 2018-06-15 | 2021-03-09 | KWS SAAT SE & Co. KGaA | Métodos para melhorar a engenharia e regeneração do genoma em planta |
US11291176B2 (en) | 2018-06-15 | 2022-04-05 | Nunhems B.V. | Seedless watermelon plants comprising modifications in an ABC transporter gene |
CA3103500A1 (en) | 2018-06-15 | 2019-12-19 | KWS SAAT SE & Co. KGaA | Methods for improving genome engineering and regeneration in plant ii |
EP3623379A1 (en) | 2018-09-11 | 2020-03-18 | KWS SAAT SE & Co. KGaA | Beet necrotic yellow vein virus (bnyvv)-resistance modifying gene |
US20220064657A1 (en) | 2019-01-04 | 2022-03-03 | Cargill, Incorporated | Engineered nucleases to generate mutations in plants |
CN113490747A (zh) | 2019-01-29 | 2021-10-08 | 华威大学 | 用于提高基因组工程化效率的方法 |
EP3708651A1 (en) | 2019-03-12 | 2020-09-16 | KWS SAAT SE & Co. KGaA | Improving plant regeneration |
EP3757219A1 (en) | 2019-06-28 | 2020-12-30 | KWS SAAT SE & Co. KGaA | Enhanced plant regeneration and transformation by using grf1 booster gene |
CA3157872A1 (en) | 2019-11-12 | 2021-05-20 | Otto Torjek | Gene for resistance to a pathogen of the genus heterodera |
CN111534538B (zh) * | 2020-05-11 | 2022-02-01 | 山西大学 | 一种快速筛选非转基因定点突变植物的方法 |
EP4019639A1 (en) | 2020-12-22 | 2022-06-29 | KWS SAAT SE & Co. KGaA | Promoting regeneration and transformation in beta vulgaris |
EP4019638A1 (en) | 2020-12-22 | 2022-06-29 | KWS SAAT SE & Co. KGaA | Promoting regeneration and transformation in beta vulgaris |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6395959B1 (en) * | 1992-05-05 | 2002-05-28 | Institut Pasteur | Nucleotide sequence encoding the enzyme I SceI and the use thereof |
US5792632A (en) * | 1992-05-05 | 1998-08-11 | Institut Pasteur | Nucleotide sequence encoding the enzyme I-SceI and the uses thereof |
US5527695A (en) * | 1993-01-29 | 1996-06-18 | Purdue Research Foundation | Controlled modification of eukaryotic genomes |
-
2001
- 2001-07-04 DE DE10131786A patent/DE10131786A1/de not_active Withdrawn
-
2002
- 2002-07-02 IL IL15928602A patent/IL159286A0/xx unknown
- 2002-07-02 WO PCT/EP2002/007281 patent/WO2003004659A2/de active Application Filing
- 2002-07-02 JP JP2003510817A patent/JP2004533267A/ja active Pending
- 2002-07-02 CN CNB028133757A patent/CN100451123C/zh not_active Expired - Lifetime
- 2002-07-02 AU AU2002325859A patent/AU2002325859B2/en not_active Expired
- 2002-07-02 EP EP02760212.7A patent/EP1407034B1/de not_active Expired - Lifetime
- 2002-07-02 BR BR0210839-9A patent/BR0210839A/pt active IP Right Grant
- 2002-07-02 BR BRPI0210839-9A patent/BRPI0210839B1/pt unknown
- 2002-07-02 MX MXPA03011844A patent/MXPA03011844A/es active IP Right Grant
- 2002-07-02 CA CA2451492A patent/CA2451492C/en not_active Expired - Lifetime
- 2002-07-03 AR ARP020102493A patent/AR034687A1/es not_active Application Discontinuation
-
2004
- 2004-01-05 US US10/750,891 patent/US7736886B2/en not_active Expired - Lifetime
-
2012
- 2012-04-23 AR ARP120101393A patent/AR086031A2/es not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
CN1628174A (zh) | 2005-06-15 |
CA2451492C (en) | 2014-12-16 |
US20050172365A1 (en) | 2005-08-04 |
MXPA03011844A (es) | 2005-03-07 |
AU2002325859B2 (en) | 2008-05-29 |
CN100451123C (zh) | 2009-01-14 |
US7736886B2 (en) | 2010-06-15 |
WO2003004659A2 (de) | 2003-01-16 |
AR034687A1 (es) | 2004-03-03 |
BR0210839A (pt) | 2004-07-13 |
WO2003004659A8 (de) | 2004-04-22 |
BRPI0210839B1 (pt) | 2019-02-26 |
WO2003004659A3 (de) | 2003-09-25 |
EP1407034A2 (de) | 2004-04-14 |
IL159286A0 (en) | 2004-06-01 |
EP1407034B1 (de) | 2016-09-07 |
CA2451492A1 (en) | 2003-01-16 |
DE10131786A1 (de) | 2003-01-16 |
JP2004533267A (ja) | 2004-11-04 |
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