MX2014008243A - Un metodo para seleccionar plantas por elementos geneticos que inducen la partenogenesis en plantas. - Google Patents

Un metodo para seleccionar plantas por elementos geneticos que inducen la partenogenesis en plantas.

Info

Publication number
MX2014008243A
MX2014008243A MX2014008243A MX2014008243A MX2014008243A MX 2014008243 A MX2014008243 A MX 2014008243A MX 2014008243 A MX2014008243 A MX 2014008243A MX 2014008243 A MX2014008243 A MX 2014008243A MX 2014008243 A MX2014008243 A MX 2014008243A
Authority
MX
Mexico
Prior art keywords
plants
seed
embryo
methods
parthenogenesis
Prior art date
Application number
MX2014008243A
Other languages
English (en)
Inventor
Shai J Lawit
Andrew Mark Cigan
Original Assignee
Pioner Hi Bred International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioner Hi Bred International Inc filed Critical Pioner Hi Bred International Inc
Publication of MX2014008243A publication Critical patent/MX2014008243A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8233—Female-specific, e.g. pistil, ovule

Abstract

Se proporcionan composiciones y métodos para la producción de una población de plantas que carecen de embriones derivados sexualmente. Las composiciones incluyen casetes de supresión que codifican polinucleótidos y promotores que resultan en partenogénesis. Se proporcionan, además, elementos genéticos para partenogénesis que se usan para impedir la reproducción sexual en una planta autoreproductora. Los métodos incluyen el uso de una mutación recesiva defectuosa para el embrión materno que después se mantiene como un sistema de mantenimiento innato estéril, lo que permite la generación de una población que es homocigota para el alelo mutante recesivo, pero transgénicamente complementada. Los métodos incluyen, además, usar un gen de una toxina expresada a través de un promotor específico de ovocélula, crear un fenotipo dominante sin embriones, no transmisible a través del gameto femenino. Las plantas homocigotas resultantes se transformarían después con un promotor de ovocélula que conduce el antídoto, un PTU de eliminación de polen y un marcador de color de la semilla para la identificación de semillas transgénicas. La generación de 50% de plantas fértiles femeninas, que tienen semillas que cuando se cultivan en la próxima generación rendirán plantas con 50% de semillas transgénicas viables, y 50% de semillas sin embrión, no viables.
MX2014008243A 2012-01-06 2012-04-12 Un metodo para seleccionar plantas por elementos geneticos que inducen la partenogenesis en plantas. MX2014008243A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261583641P 2012-01-06 2012-01-06
PCT/US2012/033235 WO2013103366A1 (en) 2012-01-06 2012-04-12 A method to screen plants for genetic elements inducing parthenogenesis in plants

Publications (1)

Publication Number Publication Date
MX2014008243A true MX2014008243A (es) 2015-02-20

Family

ID=45976553

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014008243A MX2014008243A (es) 2012-01-06 2012-04-12 Un metodo para seleccionar plantas por elementos geneticos que inducen la partenogenesis en plantas.

Country Status (7)

Country Link
US (1) US20130180005A1 (es)
EP (1) EP2800814A1 (es)
CN (1) CN104039967A (es)
BR (1) BR112014016774A2 (es)
CA (1) CA2860692A1 (es)
MX (1) MX2014008243A (es)
WO (1) WO2013103366A1 (es)

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CN108456690B (zh) * 2018-04-10 2020-08-28 华中科技大学 一种甘蓝型油菜高效遗传转化及快速鉴定方法
CN110029126B (zh) * 2019-04-17 2023-05-02 湖南杂交水稻研究中心 一种固定植物杂种优势的方法
CN110093354B (zh) * 2019-04-17 2020-06-05 湖南杂交水稻研究中心 一种固定植物杂种优势的种子分选方法
CN111328702A (zh) * 2020-04-10 2020-06-26 河南省农业科学院经济作物研究所 一种诱导产生甘蓝型油菜种子的方法及应用
CN114181965A (zh) * 2020-09-14 2022-03-15 湖南杂交水稻研究中心 核酸分子、载体、细胞和引物及其应用以及基于双重调控的植物高纯度克隆种子分选方法
CN112841027B (zh) * 2021-03-08 2022-06-28 内蒙古民族大学 一种利用聚乙烯亚胺诱导玉米无融合生殖的育种方法及应用
CN113317197B (zh) * 2021-08-03 2021-10-26 中国农业科学院生物技术研究所 一种快速显色孤雌生殖诱导系及其在鉴别玉米单倍体中的应用
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Also Published As

Publication number Publication date
BR112014016774A2 (pt) 2020-10-27
US20130180005A1 (en) 2013-07-11
CN104039967A (zh) 2014-09-10
EP2800814A1 (en) 2014-11-12
WO2013103366A1 (en) 2013-07-11
CA2860692A1 (en) 2013-07-11

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