DE602007012846D1 - Verbessertes verfahren zur homologen rekombination in eukaryontischen zellen - Google Patents

Verbessertes verfahren zur homologen rekombination in eukaryontischen zellen

Info

Publication number
DE602007012846D1
DE602007012846D1 DE602007012846T DE602007012846T DE602007012846D1 DE 602007012846 D1 DE602007012846 D1 DE 602007012846D1 DE 602007012846 T DE602007012846 T DE 602007012846T DE 602007012846 T DE602007012846 T DE 602007012846T DE 602007012846 D1 DE602007012846 D1 DE 602007012846D1
Authority
DE
Germany
Prior art keywords
homologous recombination
improved method
cells
eukaryontic
eukaryontic cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602007012846T
Other languages
English (en)
Inventor
Van Den Marco Alexan Berg
Richard Kerkman
Hesselien Touw-Riel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Publication of DE602007012846D1 publication Critical patent/DE602007012846D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/70—Vectors containing special elements for cloning, e.g. topoisomerase, adaptor sites

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Bipolar Transistors (AREA)
DE602007012846T 2006-04-08 2007-03-14 Verbessertes verfahren zur homologen rekombination in eukaryontischen zellen Active DE602007012846D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06075904 2006-04-08
PCT/EP2007/052392 WO2007115886A1 (en) 2006-04-08 2007-03-14 Improved method for homologous recombination in eukaryotic cells

Publications (1)

Publication Number Publication Date
DE602007012846D1 true DE602007012846D1 (de) 2011-04-14

Family

ID=38267581

Family Applications (2)

Application Number Title Priority Date Filing Date
DE602007005237T Active DE602007005237D1 (de) 2006-04-08 2007-03-14 Verbessertes verfahren zur homologen rekombination in pilzzellen
DE602007012846T Active DE602007012846D1 (de) 2006-04-08 2007-03-14 Verbessertes verfahren zur homologen rekombination in eukaryontischen zellen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE602007005237T Active DE602007005237D1 (de) 2006-04-08 2007-03-14 Verbessertes verfahren zur homologen rekombination in pilzzellen

Country Status (7)

Country Link
US (3) US20090124014A1 (de)
EP (2) EP2004827B1 (de)
CN (1) CN101421401A (de)
AT (2) ATE460489T1 (de)
DE (2) DE602007005237D1 (de)
DK (2) DK2004826T3 (de)
WO (2) WO2007115887A1 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
ATE480632T1 (de) 2004-04-02 2010-09-15 Dsm Ip Assets Bv Filamentöse pilzmutanten mit verbesserter homologer rekombinationseffizienz
US20090124014A1 (en) * 2006-04-08 2009-05-14 Marco Alexander Van Den Berg Method for homologous recombination in fungal cells
US20100120154A1 (en) * 2007-03-21 2010-05-13 Dsm Ip Assets B.V. Method for homologous recombination
EP2123772A1 (de) 2008-04-29 2009-11-25 DSM IP Assets B.V. Beta-Lactamantibiotikum produzierende Stämme
EP2392649A3 (de) 2008-08-05 2012-01-11 DSM IP Assets B.V. Adipoyl-7-ADCA-produzierende Stämme
BRPI1015536A2 (pt) 2009-04-24 2019-07-02 Dsm Ip Assets Bv polipeptídeo de degradação de carboidratos e usos do mesmo
US8734782B2 (en) 2009-07-22 2014-05-27 Dsm Ip Assets B.V. Host cell for the production of a compound of interest
US8802415B2 (en) 2009-11-04 2014-08-12 Dsm Ip Assets B.V. Talaromyces transformants
AU2011214327B2 (en) 2010-02-11 2014-09-18 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
US9701969B2 (en) 2010-06-03 2017-07-11 Danisco Us Inc. Filamentous fungal host strains and DNA constructs, and methods of use thereof
AU2011273688B2 (en) 2010-06-29 2014-10-30 Versalis S.P.A. Polypeptide having beta-glucosidase activity and uses thereof
EP2588601A1 (de) 2010-06-29 2013-05-08 DSM IP Assets B.V. Polypeptid mit kohlenhydratabbauender wirkung und seine verwendung
EA201300056A1 (ru) 2010-06-29 2013-07-30 ДСМ АйПи АССЕТС Б.В. Полипептид с активностью ацетилксиланэстеразы и его применения
MX2012014990A (es) 2010-06-29 2013-02-26 Dsm Ip Assets Bv Polipeptido que tiene actividad suolenina y sus usos.
BR112012033703A2 (pt) 2010-06-29 2016-10-11 Dsm Ip Assets Bv polipeptídeo tendo atividade beta-glicosidase e seus usos
CN102971419B (zh) 2010-06-29 2015-02-11 帝斯曼知识产权资产管理有限公司 具有降解碳水化合物材料的活性或帮助降解碳水化合物材料的活性的多肽及其用途
US9909152B2 (en) * 2011-11-30 2018-03-06 Battelle Memorial Institute Enhanced itaconic acid production in Aspergillus with increased LaeA expression
BR112015006995A2 (pt) * 2012-09-28 2017-07-04 Ucb Biopharma Sprl métodos para produção de proteínas recombinantes
MY187542A (en) 2013-02-04 2021-09-28 Dsm Ip Assets Bv Carbohydrate degrading polypeptide and uses thereof
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202621A1 (en) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
CN104894165B (zh) * 2015-05-26 2019-01-25 中国科学院青岛生物能源与过程研究所 一种提高基因打靶技术在土曲霉中应用效率的方法与应用
CN104894115B (zh) * 2015-05-26 2018-11-13 中国科学院青岛生物能源与过程研究所 一种具有高效同源重组能力的土曲霉及其构建方法与应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
WO1997006261A2 (en) * 1995-08-03 1997-02-20 Gist-Brocades B.V. THE USE OF HOMOLOGOUS amdS GENES AS SELECTABLE MARKERS
US6461864B1 (en) * 1998-04-15 2002-10-08 Fred Hutchinson Cancer Research Center Methods and vector constructs for making non-human animals which ubiquitously express a heterologous gene
AU1647501A (en) * 1999-12-10 2001-06-18 Cytos Biotechnology Ag Replicon based activation of endogenous genes
EP1217074A1 (de) * 2000-12-22 2002-06-26 Universiteit Leiden Integration von Nukleinsäure in Eukaryoten
DE10131786A1 (de) * 2001-07-04 2003-01-16 Sungene Gmbh & Co Kgaa Rekombinationssysteme und Verfahren zum Entfernen von Nukleinsäuresequenzen aus dem Genom eukaryotischer Organismen
US20030092183A1 (en) * 2001-09-21 2003-05-15 Fisher Katherine E. Rapid creation of gene targeting vectors using homologous recombination in yeast
CA2548220A1 (en) * 2003-12-01 2005-09-09 Introgen Therapeutics, Inc. Use of mda-7 to inhibit infection by pathogenic organisms
US20050181509A1 (en) * 2004-02-12 2005-08-18 The Penn State Research Foundation Dual selection based, targeted gene disruption method for fungi and fungus-like organisms
JP4050712B2 (ja) * 2004-02-27 2008-02-20 独立行政法人科学技術振興機構 相同組換えを行わせる方法
AU2005287547B2 (en) * 2004-09-23 2010-09-02 Basf Plant Science Gmbh Recombination cassettes and methods for sequence excision in plants
WO2007013979A2 (en) * 2005-07-20 2007-02-01 Invitrogen Corporation Methods for increasing efficiency of homologous recombination
US20090124014A1 (en) * 2006-04-08 2009-05-14 Marco Alexander Van Den Berg Method for homologous recombination in fungal cells
US8597923B2 (en) * 2009-05-06 2013-12-03 SyntheZyme, LLC Oxidation of compounds using genetically modified Candida

Also Published As

Publication number Publication date
US20090170206A1 (en) 2009-07-02
DE602007005237D1 (de) 2010-04-22
EP2004826A1 (de) 2008-12-24
ATE460489T1 (de) 2010-03-15
CN101421401A (zh) 2009-04-29
EP2004826B1 (de) 2011-03-02
ATE500333T1 (de) 2011-03-15
DK2004827T3 (da) 2010-06-14
EP2004827B1 (de) 2010-03-10
EP2004827A1 (de) 2008-12-24
US20090124014A1 (en) 2009-05-14
DK2004826T3 (da) 2011-05-16
WO2007115886A1 (en) 2007-10-18
WO2007115887A1 (en) 2007-10-18
US20120282698A1 (en) 2012-11-08

Similar Documents

Publication Publication Date Title
DE602007012846D1 (de) Verbessertes verfahren zur homologen rekombination in eukaryontischen zellen
IN2014DN09261A (de)
CO6731141A2 (es) Moléculas de ácido nucleico que tienen como objetivo la proteína de unión a gtp pequeña rho1 y que suministran resistencia contra las plagas de los coleópteros
AR084774A1 (es) Moleculas de acido nucleico que confieren resistencia a plagas coleopteros
MY194500A (en) Methods and Compositions for Producing Fatty Alcohols
EP2245155A4 (de) Verfahren zum sequentiellen austausch eines zielbereichs mittels homologer rekombination
UY33853A (es) ?moléculas de ácido nucleico que se dirigen a la subunidad h de la atpasa vacuolar y confieren resistencia a plagas de coleópteros?.
FR2906403B1 (fr) Procede de recuit de cellules photovoltaiques
DK2574677T3 (da) Fremgangsmåder til celledyrkning
BRPI0907112A2 (pt) Método para seletivamente cultivar uma alga-alvo, biocombustível, ácido poli-insaturado, matéria-prima, fitonutriente, cultura de algas seletiva em tanque de água corrente ao ar livre
DK2048955T3 (da) Målrettet gen-tilførsel til dendrit-cellevaccination
CO6731142A2 (es) Moléculas de ácido nucleico que tienen como objetivo la subunidad atpasa c vacuolar y que suministran resistencia contra las plagas de los coleópteros
IN2012DN02333A (de)
PT2427562T (pt) Cultura contínua para produção de 1,3-propanodiol usando concentração elevada de glicerina
DK2281053T3 (da) Fremstilling af biobrændstof af plantevævskulturkilder
ITMI20061974A1 (it) Anodo per elettrolisi
WO2010128024A3 (en) Reversibly immortalized cells as well as methods relating hereto
WO2013052895A3 (en) Nucleic acid molecules that target rpa70 and confer resistance to coleopteran pests
GB2477240B8 (en) Sacrificial anodes in concrete patch repair
DK2147133T3 (da) Elektrode til membranelektrolyseceller
UY32488A (es) Lipasa cpo y derivados de la misma
GB0720502D0 (en) Markers of matrix gene expression and cellular differentiation in chondrocytes
TR201106368A2 (tr) Kıkırdak dokusundan izole edilen kondrosit hücrelerinin üretilmesi için yöntem ve bununla elde edilen kondrosit kültürü.
BR112012007692A2 (pt) planta, ácido nucleico isolado, e, métodos para gerar uma planta haploide e para preparar uma planta
HU0800264D0 (en) Method for identifying sequences expected to play a specific role in eukaryotic gene regulation