ATE500333T1 - Verbessertes verfahren zur homologen rekombination in eukaryontischen zellen - Google Patents
Verbessertes verfahren zur homologen rekombination in eukaryontischen zellenInfo
- Publication number
- ATE500333T1 ATE500333T1 AT07726885T AT07726885T ATE500333T1 AT E500333 T1 ATE500333 T1 AT E500333T1 AT 07726885 T AT07726885 T AT 07726885T AT 07726885 T AT07726885 T AT 07726885T AT E500333 T1 ATE500333 T1 AT E500333T1
- Authority
- AT
- Austria
- Prior art keywords
- improved method
- cells
- eukaryontic
- homologue recombination
- homologue
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/70—Vectors containing special elements for cloning, e.g. topoisomerase, adaptor sites
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Bipolar Transistors (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP06075904 | 2006-04-08 | ||
PCT/EP2007/052392 WO2007115886A1 (en) | 2006-04-08 | 2007-03-14 | Improved method for homologous recombination in eukaryotic cells |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE500333T1 true ATE500333T1 (de) | 2011-03-15 |
Family
ID=38267581
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT07726886T ATE460489T1 (de) | 2006-04-08 | 2007-03-14 | Verbessertes verfahren zur homologen rekombination in pilzzellen |
AT07726885T ATE500333T1 (de) | 2006-04-08 | 2007-03-14 | Verbessertes verfahren zur homologen rekombination in eukaryontischen zellen |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT07726886T ATE460489T1 (de) | 2006-04-08 | 2007-03-14 | Verbessertes verfahren zur homologen rekombination in pilzzellen |
Country Status (7)
Country | Link |
---|---|
US (3) | US20090170206A1 (de) |
EP (2) | EP2004826B1 (de) |
CN (1) | CN101421401A (de) |
AT (2) | ATE460489T1 (de) |
DE (2) | DE602007005237D1 (de) |
DK (2) | DK2004827T3 (de) |
WO (2) | WO2007115886A1 (de) |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11311574B2 (en) | 2003-08-08 | 2022-04-26 | Sangamo Therapeutics, Inc. | Methods and compositions for targeted cleavage and recombination |
US7888121B2 (en) | 2003-08-08 | 2011-02-15 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
EP3351637B1 (de) | 2004-04-02 | 2020-06-10 | DSM IP Assets B.V. | Fadenpilzmutanten mit verbesserter homologer rekombinationseffizienz |
DE602007005237D1 (de) * | 2006-04-08 | 2010-04-22 | Dsm Ip Assets Bv | Verbessertes verfahren zur homologen rekombination in pilzzellen |
EP2126100B1 (de) * | 2007-03-21 | 2012-11-14 | DSM IP Assets B.V. | Verbessertes verfahren zur homologen rekombination |
EP2123772A1 (de) | 2008-04-29 | 2009-11-25 | DSM IP Assets B.V. | Beta-Lactamantibiotikum produzierende Stämme |
EP2310487A2 (de) | 2008-08-05 | 2011-04-20 | DSM IP Assets B.V. | Adipoyl-7-adca produzierende stämme |
MX2011011215A (es) | 2009-04-24 | 2012-02-21 | Dsm Ip Assets Bv | Polipeptido que degrada carbohidratos y usos del mismo. |
WO2011009700A1 (en) | 2009-07-22 | 2011-01-27 | Dsm Ip Assets B.V. | Improved host cell for the production of a compound of interest |
EP2955221A1 (de) | 2009-11-04 | 2015-12-16 | DSM IP Assets B.V. | Talaromyces-transformanten |
US9133448B2 (en) | 2010-02-11 | 2015-09-15 | Dsm Ip Assets B.V. | Polypeptide having cellobiohydrolase activity and uses thereof |
DK2576796T3 (en) * | 2010-06-03 | 2017-06-19 | Danisco Us Inc | Filamentous fungal host strains and DNA constructs, as well as procedures for their use |
EA201300060A1 (ru) | 2010-06-29 | 2013-05-30 | ДСМ АйПи АССЕТС Б.В. | Полипептид, обладающий или содействующий активности деградации углеводного материала, и его применение |
EA201300059A1 (ru) | 2010-06-29 | 2013-05-30 | ДСМ АйПи АССЕТС Б.В. | Полипептид, обладающий активностью сволленина, и его применение |
BR112012033699A2 (pt) | 2010-06-29 | 2016-10-11 | Dsm Ip Assets Bv | polipeptídeo tendo atividade beta-glicosidase e seus usos |
EA201300056A1 (ru) | 2010-06-29 | 2013-07-30 | ДСМ АйПи АССЕТС Б.В. | Полипептид с активностью ацетилксиланэстеразы и его применения |
CA2801647A1 (en) | 2010-06-29 | 2012-01-05 | Dsm Ip Assets B.V. | Polypeptide having carbohydrate degrading activity and uses thereof |
PL2588492T3 (pl) | 2010-06-29 | 2016-09-30 | Polipeptyd mający aktywność beta-glukozydazy i jego zastosowania | |
US9909152B2 (en) * | 2011-11-30 | 2018-03-06 | Battelle Memorial Institute | Enhanced itaconic acid production in Aspergillus with increased LaeA expression |
JP2015530103A (ja) * | 2012-09-28 | 2015-10-15 | ユーシービー バイオファルマ エスピーアールエル | 組換えタンパク質を生産するための方法 |
HRP20220917T1 (hr) | 2013-02-04 | 2022-10-28 | Dsm Ip Assets B.V. | Polipeptid koji razgrađuje ugljikohidrate i njegova upotreba |
WO2014202620A2 (en) | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
WO2014202624A2 (en) | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
WO2014202622A2 (en) | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
WO2014202621A1 (en) | 2013-06-20 | 2014-12-24 | Dsm Ip Assets B.V. | Carbohydrate degrading polypeptide and uses thereof |
CN104894165B (zh) * | 2015-05-26 | 2019-01-25 | 中国科学院青岛生物能源与过程研究所 | 一种提高基因打靶技术在土曲霉中应用效率的方法与应用 |
CN104894115B (zh) * | 2015-05-26 | 2018-11-13 | 中国科学院青岛生物能源与过程研究所 | 一种具有高效同源重组能力的土曲霉及其构建方法与应用 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5464764A (en) * | 1989-08-22 | 1995-11-07 | University Of Utah Research Foundation | Positive-negative selection methods and vectors |
DK0758020T3 (da) * | 1995-08-03 | 2009-04-06 | Dsm Ip Assets Bv | Amds-gen fra Aspergillus niger kodende for en acetamidase |
US6461864B1 (en) * | 1998-04-15 | 2002-10-08 | Fred Hutchinson Cancer Research Center | Methods and vector constructs for making non-human animals which ubiquitously express a heterologous gene |
AU1647501A (en) * | 1999-12-10 | 2001-06-18 | Cytos Biotechnology Ag | Replicon based activation of endogenous genes |
EP1217074A1 (de) * | 2000-12-22 | 2002-06-26 | Universiteit Leiden | Integration von Nukleinsäure in Eukaryoten |
DE10131786A1 (de) * | 2001-07-04 | 2003-01-16 | Sungene Gmbh & Co Kgaa | Rekombinationssysteme und Verfahren zum Entfernen von Nukleinsäuresequenzen aus dem Genom eukaryotischer Organismen |
US20030092183A1 (en) * | 2001-09-21 | 2003-05-15 | Fisher Katherine E. | Rapid creation of gene targeting vectors using homologous recombination in yeast |
US8034790B2 (en) * | 2003-12-01 | 2011-10-11 | Introgen Therapeutics | Use of MDA-7 to inhibit pathogenic infectious organisms |
US20050181509A1 (en) * | 2004-02-12 | 2005-08-18 | The Penn State Research Foundation | Dual selection based, targeted gene disruption method for fungi and fungus-like organisms |
JP4050712B2 (ja) * | 2004-02-27 | 2008-02-20 | 独立行政法人科学技術振興機構 | 相同組換えを行わせる方法 |
EP1794305A2 (de) * | 2004-09-23 | 2007-06-13 | BASF Plant Science GmbH | Rekombinationskassetten und verfahren zur sequenz-exzision in pflanzen |
US20070155014A1 (en) * | 2005-07-20 | 2007-07-05 | Invitrogen Corporation | Methods for increasing efficiency of homologous recombination |
DE602007005237D1 (de) * | 2006-04-08 | 2010-04-22 | Dsm Ip Assets Bv | Verbessertes verfahren zur homologen rekombination in pilzzellen |
US8597923B2 (en) * | 2009-05-06 | 2013-12-03 | SyntheZyme, LLC | Oxidation of compounds using genetically modified Candida |
-
2007
- 2007-03-14 DE DE602007005237T patent/DE602007005237D1/de active Active
- 2007-03-14 DK DK07726886.0T patent/DK2004827T3/da active
- 2007-03-14 DK DK07726885.2T patent/DK2004826T3/da active
- 2007-03-14 EP EP07726885A patent/EP2004826B1/de not_active Not-in-force
- 2007-03-14 WO PCT/EP2007/052392 patent/WO2007115886A1/en active Application Filing
- 2007-03-14 CN CNA2007800127373A patent/CN101421401A/zh active Pending
- 2007-03-14 EP EP07726886A patent/EP2004827B1/de not_active Not-in-force
- 2007-03-14 AT AT07726886T patent/ATE460489T1/de active
- 2007-03-14 AT AT07726885T patent/ATE500333T1/de active
- 2007-03-14 US US12/296,506 patent/US20090170206A1/en not_active Abandoned
- 2007-03-14 DE DE602007012846T patent/DE602007012846D1/de active Active
- 2007-03-14 US US12/296,503 patent/US20090124014A1/en not_active Abandoned
- 2007-03-14 WO PCT/EP2007/052393 patent/WO2007115887A1/en active Application Filing
-
2012
- 2012-05-11 US US13/469,762 patent/US20120282698A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP2004827A1 (de) | 2008-12-24 |
EP2004827B1 (de) | 2010-03-10 |
DK2004826T3 (da) | 2011-05-16 |
WO2007115887A1 (en) | 2007-10-18 |
DK2004827T3 (da) | 2010-06-14 |
EP2004826B1 (de) | 2011-03-02 |
US20090124014A1 (en) | 2009-05-14 |
ATE460489T1 (de) | 2010-03-15 |
DE602007012846D1 (de) | 2011-04-14 |
WO2007115886A1 (en) | 2007-10-18 |
CN101421401A (zh) | 2009-04-29 |
US20090170206A1 (en) | 2009-07-02 |
EP2004826A1 (de) | 2008-12-24 |
DE602007005237D1 (de) | 2010-04-22 |
US20120282698A1 (en) | 2012-11-08 |
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