AR081594A1 - Metodos y medios para modificar un genoma vegetal en una secuencia de nucleotidos usada comunmente en la ingenieria genetica de genomas vegetales - Google Patents

Metodos y medios para modificar un genoma vegetal en una secuencia de nucleotidos usada comunmente en la ingenieria genetica de genomas vegetales

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Publication number
AR081594A1
AR081594A1 ARP110101986A ARP110101986A AR081594A1 AR 081594 A1 AR081594 A1 AR 081594A1 AR P110101986 A ARP110101986 A AR P110101986A AR P110101986 A ARP110101986 A AR P110101986A AR 081594 A1 AR081594 A1 AR 081594A1
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Argentina
Prior art keywords
plant
predefined site
foreign dna
introducing
genome
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ARP110101986A
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English (en)
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Kathleen Dhalluin
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Bayer Bioscience Nv
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Publication of AR081594A1 publication Critical patent/AR081594A1/es

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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Pest Control & Pesticides (AREA)
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Abstract

Reivindicación 1: Un método para introducir una molécula de ADN extraño en un sitio predefinido en un genoma de una célula vegetal, caracterizado porque comprende los pasos de a) inducir una ruptura de ADN de cadena doble en dicho sitio predefinido; b) introducir dicha molécula de ADN extraño en dicha célula vegetal; y c) seleccionar una célula vegetal en donde dicho ADN extraño se introduce en dicho sitio predefinido; y caracterizado porque dicho sitio predefinido está comprendido dentro una región de ADN que codifica una fosfinotricina-cetiltransferasa codificada por Streptomyces hygroscopicus (región de codificación de bar) y que dicha ruptura de ADN de cadena doble es inducida por introducción de una meganucleasa de cadena simple o por un par de meganucleasas que reconoce o reconocen conjuntamente dicho sitio predefinido e induce o inducen dicha ruptura de cadena doble.Reivindicación 21: Una planta, caracterizada porque dicho ADN extraño se introdujo en dicho sitio predefinido, obtenido mediante el método de acuerdo con la reivindicación 19. Reivindicación 22: Una semilla o una parte de propagación de una planta, caracterizada porque comprende dicho ADN extraño integrado en dicho sitio predefinido, de la planta de la reivindicación 21. Reivindicación 23: Un método para cultivar una planta de acuerdo con la reivindicación 21, caracterizado porque comprende el paso de aplicar un compuesto químico a dicha planta o sustrato en donde se cultiva dicha planta. Reivindicación 24: Un proceso para producir una planta que comprende ADN extraño integrado en la región de codificación de bar, caracterizado porque comprende el paso de cruzar una planta que consiste esencialmente de las células vegetales de la reivindicación 20 con otra planta o consigo misma y opcionalmente cosechar las semillas. Reivindicación 28: Uso de una meganucleasa personalizada, caracterizado porque es para introducir un ADN extraño de interés en un sitio predefinido en una célula vegetal.
ARP110101986A 2010-06-09 2011-06-09 Metodos y medios para modificar un genoma vegetal en una secuencia de nucleotidos usada comunmente en la ingenieria genetica de genomas vegetales AR081594A1 (es)

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EP10005941 2010-06-09
US35584910P 2010-06-17 2010-06-17

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AR081594A1 true AR081594A1 (es) 2012-10-03

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ARP110101986A AR081594A1 (es) 2010-06-09 2011-06-09 Metodos y medios para modificar un genoma vegetal en una secuencia de nucleotidos usada comunmente en la ingenieria genetica de genomas vegetales

Country Status (14)

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US (2) US9574201B2 (es)
EP (1) EP2580335B1 (es)
JP (1) JP6092100B2 (es)
KR (1) KR101995698B1 (es)
CN (2) CN103119169B (es)
AR (1) AR081594A1 (es)
AU (1) AU2011264075B2 (es)
BR (1) BR112012031323A2 (es)
CA (1) CA2801836A1 (es)
MX (1) MX347575B (es)
RU (1) RU2639512C2 (es)
SG (1) SG185668A1 (es)
UA (1) UA115961C2 (es)
WO (1) WO2011154159A1 (es)

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EP2580335A1 (en) 2013-04-17
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RU2639512C2 (ru) 2017-12-21
CN103119169B (zh) 2018-11-20
BR112012031323A2 (pt) 2015-09-08
US9574201B2 (en) 2017-02-21
US20170096674A1 (en) 2017-04-06
UA115961C2 (uk) 2018-01-25
AU2011264075B2 (en) 2015-01-29
JP2013531489A (ja) 2013-08-08
SG185668A1 (en) 2012-12-28
JP6092100B2 (ja) 2017-03-08
EP2580335B1 (en) 2017-05-10
CN109504700A (zh) 2019-03-22
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US20130145490A1 (en) 2013-06-06
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