BR112017015368A2 - método para a modificação precisa da planta através da expressão transiente do gene. - Google Patents

método para a modificação precisa da planta através da expressão transiente do gene.

Info

Publication number
BR112017015368A2
BR112017015368A2 BR112017015368A BR112017015368A BR112017015368A2 BR 112017015368 A2 BR112017015368 A2 BR 112017015368A2 BR 112017015368 A BR112017015368 A BR 112017015368A BR 112017015368 A BR112017015368 A BR 112017015368A BR 112017015368 A2 BR112017015368 A2 BR 112017015368A2
Authority
BR
Brazil
Prior art keywords
plant
gene
transient expression
specific
site
Prior art date
Application number
BR112017015368A
Other languages
English (en)
Other versions
BR112017015368B1 (pt
Inventor
Gao Caixia
Liu Jinxing
Zhang Kang
Wang Yanpeng
Zhang Yi
Original Assignee
Inst Genetics & Developmental Biology Cas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Genetics & Developmental Biology Cas filed Critical Inst Genetics & Developmental Biology Cas
Publication of BR112017015368A2 publication Critical patent/BR112017015368A2/pt
Publication of BR112017015368B1 publication Critical patent/BR112017015368B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/14—Plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

a presente invenção refere-se a um método para a realização da modificações sítio-específica em uma planta, compreendendo as seguintes etapa: expressando de forma transiente uma nuclease específica de sequência para o fragmento alvo na célula ou no tecido da planta de interesse, onde a nuclease específica de sequência é específica para o sítio alvo e o sítio alvo é clivado pela nuclease, assim a modificação sítio-específica do sítio alvo é obtida através da reparação do dna da planta
BR112017015368-8A 2015-01-19 2016-01-19 Método para a modificação precisa da planta através da expressão transiente do gene BR112017015368B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201510025857.3 2015-01-19
CN201510025857 2015-01-19
PCT/CN2016/071352 WO2016116032A1 (en) 2015-01-19 2016-01-19 A method for precise modification of plant via transient gene expression

Publications (2)

Publication Number Publication Date
BR112017015368A2 true BR112017015368A2 (pt) 2018-01-16
BR112017015368B1 BR112017015368B1 (pt) 2024-07-16

Family

ID=

Also Published As

Publication number Publication date
EP3253879A1 (en) 2017-12-13
EP3253879A4 (en) 2018-06-20
JP7239266B2 (ja) 2023-03-14
CN105802991A (zh) 2016-07-27
JP2021061868A (ja) 2021-04-22
JP2018502590A (ja) 2018-02-01
KR20170098952A (ko) 2017-08-30
AR103446A1 (es) 2017-05-10
AU2016208913B2 (en) 2022-02-24
CN105802991B (zh) 2021-06-29
KR102085189B1 (ko) 2020-04-28
US20180073035A1 (en) 2018-03-15
EA201791633A1 (ru) 2018-03-30
WO2016116032A1 (en) 2016-07-28
CA2973750A1 (en) 2016-07-28
AU2016208913A1 (en) 2017-07-06

Similar Documents

Publication Publication Date Title
BR112017015988A2 (pt) método para a modificação dirigida a sítio de planta inteira através da expressão transiente de gene
BR112013024337A2 (pt) locus de traço transgênico complexo em uma planta, planta ou semente, método para produzir em uma planta um locus de traço transgênico complexo e construto de expressão
BR112017007083A2 (pt) moléculas de ácido nucleico subunidade copi coatomer delta que confere resistência a pragas de coleóptero e hemípteros
MX2018004809A (es) Metodos y composiciones para la modificacion genomica exenta de marcadores.
BR112017016431A2 (pt) método para realização de modificação direcionada de sítio para genomas de plantas através de uso de materiais não herdados
BR112017018327A2 (pt) método para a expressão regulada de um complexo, planta ou célula vegetal, semente, método para modificação de uma sequência, método para alteração da expressão de pelo menos um polinucleotídeo
BR112017024754A2 (pt) método e construtos para a edição de ácido nucléico direcionada em plantas
EA201791633A1 (ru) Способ точной модификации растения посредством транзиентной экспрессии гена
DK3209315T3 (da) Sammensætning omfattende en blanding af planteekstrakter eller en blanding af molekyler indeholdt i de nævnte planter, og anvendelse til styring af glucid- og/eller lipid-metabolisme
BR112018009986A2 (pt) composições de indução haploide e métodos para sua utilização
BR112016010175A2 (pt) Repressor genético, seus usos, e composição farmacêutica
BR112016012870A8 (pt) composições e métodos para melhorar a produção de alface
UY33852A (es) ?moléculas de ácido nucleico que se dirigen a la proteína de unión a gtp pequeña de rho1 y que confieren resistencia a las plagas de coleópteros?.
BR112016026136A2 (pt) composições e métodos para aumentar o desenvolvimento e o rendimento vegetal
BR112017017279A2 (pt) métodos para introduzir um gene inibidor de pólen, para introduzir dois genes inibidores de pólen, para introduzir dois genes marcadores coloridos e de introgressão acelerada de traço e planta
PH12016501191A1 (en) Ras opposite (rop) and related nucleic acid molecules that confer resistance to coleopteran and/or hemipteran pests
BR112015021210B8 (pt) Método para reduzir a expressão de um ácido nucleico alvo em uma planta e ácido nucleico recombinante
NZ725789A (en) Dre4 nucleic acid molecules that confer resistance to coleopteran pests
BR112017012407A2 (pt) supressão de rnai parental de genes de remodelagem de cromatina para controlar pragas coleópteras
CL2017000312A1 (es) Elección de microarn como diana para trastornos metabólicos
PH12016501170A1 (en) Rnapii-140 nucleic acid molecules that confer resistance to coleopteran pests
BR112017028448A2 (pt) análogos de cisteamina resistentes a ado e usos dos mesmos
FI20145293A (fi) Leukamurskain, murskauslaitos ja menetelmä leukamurskaimen käyttämiseksi
BR112016030728A2 (pt) método para tratar ou prevenir uma inflamação induzida por bactérias em um indivíduo, composição imunogênica, composição farmacêutica, uso da composição imunogênica, método para produzir uma composição imunogênica, e, método para tratar ou prevenir uma doença
BR112017012389A2 (pt) supressão de rnai parental de gene kruppel para controlar pragas de coleóptero

Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 19/01/2016, OBSERVADAS AS CONDICOES LEGAIS

B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: REFERENTE A RPI 2793 DE 16/07/2024, QUANTO AO ITEM (54) TITULO.