BR112015021210B8 - Método para reduzir a expressão de um ácido nucleico alvo em uma planta e ácido nucleico recombinante - Google Patents

Método para reduzir a expressão de um ácido nucleico alvo em uma planta e ácido nucleico recombinante

Info

Publication number
BR112015021210B8
BR112015021210B8 BR112015021210A BR112015021210A BR112015021210B8 BR 112015021210 B8 BR112015021210 B8 BR 112015021210B8 BR 112015021210 A BR112015021210 A BR 112015021210A BR 112015021210 A BR112015021210 A BR 112015021210A BR 112015021210 B8 BR112015021210 B8 BR 112015021210B8
Authority
BR
Brazil
Prior art keywords
nucleic acid
expression
plant
reducing
target nucleic
Prior art date
Application number
BR112015021210A
Other languages
English (en)
Other versions
BR112015021210B1 (pt
BR112015021210A2 (pt
Inventor
e jacobsen Steve
Law Julie
Johnson Lianna
Gallego-Bartolomé Javier
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California filed Critical Univ California
Publication of BR112015021210A2 publication Critical patent/BR112015021210A2/pt
Publication of BR112015021210B1 publication Critical patent/BR112015021210B1/pt
Publication of BR112015021210B8 publication Critical patent/BR112015021210B8/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

MÉTODOS E COMPOSIÇÕES PARA O DIRECIONAMENTO DE RNA POLIMERASES E BIOGÊNSE DE RNA NÃO CODIFICANTE PARA LOCAIS ESPECÍFICOS. A presente divulgação refere-se às proteínas recombinantes que induzem silenciamento de gene epigenético e os métodos de uso de tais proteínas para reduzir a expressão de genes em plantas.
BR112015021210A 2013-03-01 2014-02-28 Método para reduzir a expressão de um ácido nucleico alvo em uma planta e ácido nucleico recombinante BR112015021210B8 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361771743P 2013-03-01 2013-03-01
US61/771,743 2013-03-01
US201461929414P 2014-01-20 2014-01-20
US61/929,414 2014-01-20
PCT/US2014/019666 WO2014134567A1 (en) 2013-03-01 2014-02-28 Methods and compositions for targeting rna polymerases and non-coding rna biogenesis to specific loci

Publications (3)

Publication Number Publication Date
BR112015021210A2 BR112015021210A2 (pt) 2017-10-10
BR112015021210B1 BR112015021210B1 (pt) 2022-10-18
BR112015021210B8 true BR112015021210B8 (pt) 2022-11-16

Family

ID=51428873

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015021210A BR112015021210B8 (pt) 2013-03-01 2014-02-28 Método para reduzir a expressão de um ácido nucleico alvo em uma planta e ácido nucleico recombinante

Country Status (5)

Country Link
US (2) US10351867B2 (pt)
EP (2) EP2961845A4 (pt)
CN (2) CN105189760A (pt)
BR (1) BR112015021210B8 (pt)
WO (1) WO2014134567A1 (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2961845A4 (en) * 2013-03-01 2016-08-10 Univ California METHODS AND COMPOSITIONS FOR TARGETING RNA POLYMERASES AND NON-CODING RNA BIOGENESIS FOR SPECIFIC LOCI
WO2017205837A1 (en) 2016-05-27 2017-11-30 The Regents Of The Univeristy Of California Methods and compositions for targeting rna polymerases and non-coding rna biogenesis to specific loci
AU2017355507B2 (en) 2016-11-04 2023-09-21 Flagship Pioneering Innovations V. Inc. Novel plant cells, plants, and seeds
CA3051585A1 (en) 2017-01-28 2018-08-02 Inari Agriculture, Inc. Novel plant cells, plants, and seeds
US11926835B1 (en) 2018-01-29 2024-03-12 Inari Agriculture Technology, Inc. Methods for efficient tomato genome editing
US11866719B1 (en) 2018-06-04 2024-01-09 Inari Agriculture Technology, Inc. Heterologous integration of regulatory elements to alter gene expression in wheat cells and wheat plants
WO2020022461A1 (ja) * 2018-07-25 2020-01-30 国立研究開発法人産業技術総合研究所 植物における標的ｄｎａのメチル化を抑制する方法
EP3894566A4 (en) * 2018-12-13 2022-08-31 Synthetic Genomics, Inc. AVOIDING EPIGENETIC SILENCING OF EXOGENE NUCLEIC ACID IN ALGAE
CN110054670A (zh) * 2019-04-04 2019-07-26 华中农业大学 一种可减小作物叶夹角的蛋白及其应用
CN114835816B (zh) * 2021-01-14 2023-12-22 中国科学院遗传与发育生物学研究所 一种调控植物基因组dna特定区域甲基化水平的方法
WO2023028598A1 (en) * 2021-08-26 2023-03-02 Donald Danforth Plant Science Center Engineering disease resistance by editing the epigenome

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US5641876A (en) 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
DE69133128T2 (de) 1990-04-12 2003-06-18 Syngenta Participations Ag Gewebe-spezifische Promotoren
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
DE69230290T2 (de) 1991-08-27 2000-07-20 Novartis Ag Proteine mit insektiziden eigenschaften gegen homopteran insekten und ihre verwendung im pflanzenschutz
JP2952041B2 (ja) 1992-07-27 1999-09-20 パイオニア ハイ−ブレッド インターナショナル，インコーポレイテッド 培養ダイズ細胞のａｇｒｏｂａｃｔｅｒｉｕｍ媒介形質転換の改良法
US5470353A (en) 1993-10-20 1995-11-28 Hollister Incorporated Post-operative thermal blanket
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5750868A (en) 1994-12-08 1998-05-12 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
US5967213A (en) 1998-02-11 1999-10-19 Springs Window Fashions Division, Inc. Window cornice assembly
AU770893B2 (en) * 1999-02-09 2004-03-04 Rhobio A method for inhibiting the expression of target genes in plants
GB0117964D0 (en) * 2001-07-24 2001-09-19 Imp College Innovations Ltd Control of gene expression
CA2567087A1 (en) * 2004-06-09 2005-12-22 E.I. Du Pont De Nemours And Company Recombinant constructs for use in reducing gene expression
KR101034562B1 (ko) * 2009-06-03 2011-05-12 경희대학교 산학협력단 배추 유래의 ｓｕ（ｖａｒ）３―９ 호몰로그 ２ 단백질 및 이를 코딩하는 유전자
WO2011049627A1 (en) * 2009-10-22 2011-04-28 Dow Agrosciences Llc Engineered zinc finger proteins targeting plant genes involved in fatty acid biosynthesis
MX2012010479A (es) 2010-03-08 2012-10-09 Monsanto Technology Llc Moleculas polinucleotidicas para regulacion genetica en plantas.
EP2961845A4 (en) * 2013-03-01 2016-08-10 Univ California METHODS AND COMPOSITIONS FOR TARGETING RNA POLYMERASES AND NON-CODING RNA BIOGENESIS FOR SPECIFIC LOCI

Also Published As

Publication number Publication date
EP3181694A1 (en) 2017-06-21
US20190390211A1 (en) 2019-12-26
US11479781B2 (en) 2022-10-25
CN106995820B (zh) 2020-12-01
CN106995820A (zh) 2017-08-01
US20160017348A1 (en) 2016-01-21
WO2014134567A1 (en) 2014-09-04
BR112015021210B1 (pt) 2022-10-18
CN105189760A (zh) 2015-12-23
EP2961845A4 (en) 2016-08-10
BR112015021210A2 (pt) 2017-10-10
US10351867B2 (en) 2019-07-16
EP2961845A1 (en) 2016-01-06

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