CN106995820A - 用于将rna聚合酶和非编码rna生物发生靶向特定基因座的方法和组合物 - Google Patents

用于将rna聚合酶和非编码rna生物发生靶向特定基因座的方法和组合物 Download PDF

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CN106995820A
CN106995820A CN201710084543.XA CN201710084543A CN106995820A CN 106995820 A CN106995820 A CN 106995820A CN 201710084543 A CN201710084543 A CN 201710084543A CN 106995820 A CN106995820 A CN 106995820A
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史蒂夫·E·雅各布森
贾维尔·加列戈斯
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]

Abstract

本申请涉及用于将RNA聚合酶和非编码RNA生物发生靶向特定基因座的方法和组合物。本公开内容涉及引起表观遗传的基因沉默的重组蛋白，并涉及使用此类蛋白降低植物中基因表达的方法。

Description

用于将RNA聚合酶和非编码RNA生物发生靶向特定基因座的方 法和组合物
本申请是申请号为201480024967.1，申请日为2014年2月28日，发明名称为“用于将RNA聚合酶和非编码RNA生物发生靶向特定基因座的方法和组合物”的申请的分案申请。
相关申请的交叉引用
本申请要求2013年3月1日提交的美国临时申请号61/771,743和2014年1月20日提交的美国临时申请号61/929,414的权益，这些申请通过引用以其整体特此并入本文。
关于联邦赞助的研究的声明
本发明根据由美国国立卫生研究院(National Institute of Health)授予的合约号GM60398在政府支持下进行。政府在本发明中具有某些权利。
以ASCII文本文件的序列表提交
以下以ASCII文本文件提交的内容通过引用以其整体并入本文：计算机可读形式(CRF)的序列表(文件名：262232000440SEQLISTING.txt，记录的日期：2014年2月28日，大小:712KB)。
领域
本公开内容涉及引起表观遗传的基因沉默的重组蛋白并涉及利用此类蛋白用于减少植物中基因表达的方法。
背景
表观遗传标志是酶介导的DNA和其相关的染色质蛋白的化学修饰。尽管表观遗传标志不改变DNA的一级序列，它们的确包含可遗传的信息并在调节基因组功能中起关键作用。此类修饰，包括胞嘧啶甲基化、组蛋白尾部和组蛋白核心的翻译后修饰、以及核小体(DNA包裹的组蛋白八聚体)的定位，影响染色质的转录状态和其他功能方面。例如，DNA和组蛋白H3N末端尾部上某些残基诸如H3赖氨酸9(H3K9)的甲基化，对于转录基因沉默和异染色质的形成是重要的。此类标志对于非基因序列的沉默是重要的，所述非基因序列包括转座子、假基因、重复序列和整合的病毒，所述非基因序列如果被表达并因此被活化会变得对细胞有害。表观遗传基因沉默在发育现象诸如植物和哺乳动物中的印记中以及在细胞分化和重编程中也是重要的。
参与表观遗传沉默的不同途径此前已被描述，并包括组蛋白脱乙酰化、H3K27和H3K9甲基化、H3K4脱甲基化、以及启动子的DNA甲基化。实现DNA甲基化的一种手段是经由称为RNA指导的DNA甲基化的现象，其中非编码RNA发挥作用以指导DNA序列的甲基化。在植物中，没有被描述的将特定DNA序列的识别与表观遗传状态的建立联系起来的蛋白。因此，植物表观遗传调节子通常不能用于植物中特定基因或转基因的表观遗传沉默。
一种解决方案是鉴定或工程化包含序列特异性锌指结构域的表观遗传调节子，因为锌指首先被鉴定为DNA结合基序(Miller等,1985)，且它们的许多其他变化已被表征。已经进行了最近的进展，其允许工程化特异性识别任何期望的DNA序列的DNA结合蛋白。例如，最近显示，三-指锌指蛋白可被构建以阻断被转化到小鼠细胞系中的人类癌基因的表达(Choo和Klug,1994)。然而，工程化包含工程化的锌指结构域的表观遗传调节子的潜在问题包括确保工程化的蛋白将具有正确的折叠以行使功能，和确保锌指结构域与表观遗传调节子的融合不会干扰锌指结构域的DNA特异性结合或表观遗传调节子的活性。
因此，对能够结合特定DNA序列、正确地折叠、且当在植物中表达时保留序列特异性DNA结合活性和表观遗传基因沉默活性二者的改进的表观遗传调节子存在需求。
概述
本公开内容涉及用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA-结合结构域，所述第二氨基酸序列包含SHH1多肽或其片段；以及b)在其中由所述重组核酸编码的重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。在一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域(realhomology domain)、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含3个C2H2锌指结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含同源结构域或SAWADEE结构域中的至少一个。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:1至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:1 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽与RNA聚合酶相互作用。在一些实施方案中，RNA聚合酶为RNA聚合酶IV。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽与甲基化H3K9相互作用。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽与H3K9me1、H3K9me2和/或H3K9me3相互作用。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽引起RNA指导的DNA甲基化。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为内源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸被沉默。
本公开内容还涉及编码包含第一氨基酸序列和第二氨基酸序列的SHH1样蛋白的重组核酸，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含SHH1多肽或其片段。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:1至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:1 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在一些实施方案中，一种或更多种靶核酸为编码多肽的核酸。在一些实施方案中，一种或更多种靶核酸为内源植物核酸。在一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，SHH1样蛋白减少一种或更多种靶核酸的表达。在可与前述实施方案的任一个组合的一些实施方案中，SHH1样蛋白使一种或更多种靶核酸的表达沉默。
本公开内容的其他方面涉及包含重组核酸的载体，所述重组核酸编码前述实施方案的任一个的SHH1样蛋白，其中所述重组核酸被可操作地连接至调节序列。本公开内容的其他方面涉及包含前述实施方案的表达载体的宿主细胞。在某些实施方案中，宿主细胞为植物细胞。本公开内容的其他方面涉及包含前述实施方案的任一个的重组核酸的重组植物。
本公开内容还涉及用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA-结合结构域，所述第二氨基酸序列包含SUVH2多肽或其片段、或SUVH9多肽或其片段；以及b)在其中由所述重组核酸编码的重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。在一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含3个C2H2锌指结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含选自双螺旋束结构域、SRA结构域、pre-SET结构域或SET结构域的结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:14或SEQ ID NO:27至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:14或SEQ ID NO:27 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽与RNA聚合酶相互作用。在一些实施方案中，RNA聚合酶为RNA聚合酶V。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽引起RNA指导的DNA甲基化。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为内源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸的表达被沉默。在可与前述实施方案的任一个组合的一些实施方案中，所述方法包括提供还包含另外的重组核酸的植物，其中所述另外的重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA-结合结构域，所述第二氨基酸序列包含SHH1多肽或其片段；以及在其中由所述另外的重组核酸编码的另外的重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。
本公开内容还涉及编码包含第一氨基酸序列和第二氨基酸序列的SUVH2样蛋白或SUVH9样蛋白的重组核酸，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含SUVH2多肽或其片段、或SUVH9多肽或其片段。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:14或SEQ ID NO:27至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:14或SEQ ID NO:27 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在一些实施方案中，一种或更多种靶核酸为编码多肽的核酸。在一些实施方案中，一种或更多种靶核酸为内源植物核酸。在一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，SUVH2样蛋白和/或SUVH9样蛋白减少一种或更多种靶核酸的表达。在可与前述实施方案的任一个组合的一些实施方案中，SUVH2样蛋白和/或SUVH9样蛋白使一种或更多种靶核酸的表达沉默。
本公开内容的其他方面涉及包含重组核酸的载体，所述重组核酸编码前述实施方案的任一个的SUVH2样蛋白和/或SUVH9样蛋白，其中所述重组核酸被可操作地连接至调节序列。本公开内容的其他方面涉及包含前述实施方案的表达载体的宿主细胞。在某些实施方案中，宿主细胞为植物细胞。本公开内容的其他方面涉及包含前述实施方案的任一个的重组核酸的重组植物。
本公开内容还涉及用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：a)提供包含第一重组核酸和第二重组核酸的植物，所述第一重组核酸编码包含第一氨基酸序列和第二氨基酸序列的第一重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含SHH1多肽或其片段，所述第二重组核酸编码包含第一氨基酸序列和第二氨基酸序列的第二重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含SUVH2多肽或其片段、或SUVH9多肽或其片段；以及b)在其中由所述第一重组核酸编码的第一重组多肽和由所述第二重组核酸编码的第二重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。在一些实施方案中，重组多肽的至少一个的DNA结合结构域包含锌指结构。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，来自重组多肽的至少一个的DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽的至少一个的DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽的至少一个的DNA结合结构域包含3个C2H2锌指结构域。在可与前述实施方案的任一个组合的一些实施方案中，第一重组多肽的第二氨基酸序列包含同源结构域或SAWADEE结构域中的至少一个。在可与前述实施方案的任一个组合的一些实施方案中，第一重组多肽的第二氨基酸序列包含与SEQID NO:1至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，第一重组多肽的第二氨基酸序列包含与SEQ ID NO:1 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽中的至少一个与RNA聚合酶相互作用。在一些实施方案中，RNA聚合酶为RNA聚合酶IV。在可与前述实施方案的任一个组合的一些实施方案中，第一重组多肽与甲基化H3K9相互作用。在可与前述实施方案的任一个组合的一些实施方案中，第一重组多肽与H3K9me1、H3K9me2和/或H3K9me3相互作用。在可与前述实施方案的任一个组合的一些实施方案中，第一重组多肽引起RNA指导的DNA甲基化。在可与前述实施方案的任一个组合的一些实施方案中，第二重组多肽的第二氨基酸序列包含选自双螺旋束结构域、SRA结构域、pre-SET结构域或SET结构域的结构域。在可与前述实施方案的任一个组合的一些实施方案中，来自第二重组多肽的第二氨基酸序列包含与SEQ ID NO:14或SEQ ID NO:27至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，第二重组多肽的第二氨基酸序列包含与SEQ ID NO:14或SEQID NO:27 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，第二重组多肽与RNA聚合酶相互作用。在一些实施方案中，RNA聚合酶为RNA聚合酶V。在可与前述实施方案的任一个组合的一些实施方案中，第二重组多肽引起RNA指导的DNA甲基化。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽的至少一个引起RNA指导的DNA甲基化。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为内源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸被沉默。
本公开内容的其他方面涉及包含重组核酸的载体，所述重组核酸编码前述实施方案的任一个的SHH1样蛋白、SUVH2样蛋白和/或SUVH9样蛋白，其中所述重组核酸被可操作地连接至调节序列。本公开内容的其他方面涉及包含前述实施方案的表达载体的宿主细胞。在某些实施方案中，宿主细胞为植物细胞。本公开内容的其他方面涉及包含前述实施方案的任一个的重组核酸的重组植物。
本公开内容还涉及用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA-结合结构域，所述第二氨基酸序列包含DMS3多肽或其片段；以及b)在其中由所述重组核酸编码的重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。在一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含3个C2H2锌指结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含选自双螺旋束结构域、SRA结构域、pre-SET结构域或SET结构域的结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:41至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:41 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽与RNA聚合酶相互作用。在可与前述实施方案的任一个组合的一些实施方案中，RNA聚合酶为RNA聚合酶V。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽引起RNA指导的DNA甲基化。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为内源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸的表达被沉默。
本公开内容还涉及编码包含第一氨基酸序列和第二氨基酸序列的DMS3样蛋白的重组核酸，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含DMS3多肽或其片段。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:41至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:41 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在一些实施方案中，一种或更多种靶核酸为编码多肽的核酸。在一些实施方案中，一种或更多种靶核酸为内源植物核酸。在一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，DMS3样蛋白减少一种或更多种靶核酸的表达。在可与前述实施方案的任一个组合的一些实施方案中，DMS3样蛋白使一种或更多种靶核酸的表达沉默。
本公开内容还涉及用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含MORC6多肽或其片段；以及b)在其中由所述重组核酸编码的重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。在一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含3个C2H2锌指结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含选自双螺旋束结构域、SRA结构域、pre-SET结构域或SET结构域的结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:53至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:53 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽与RNA聚合酶相互作用。在可与前述实施方案的任一个组合的一些实施方案中，RNA聚合酶为RNA聚合酶V。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽引起RNA指导的DNA甲基化。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为内源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸的表达被沉默。
本公开内容还涉及编码包含第一氨基酸序列和第二氨基酸序列的MORC6样蛋白的重组核酸，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含MORC6多肽或其片段。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:53至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:53 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在一些实施方案中，一种或更多种靶核酸为编码多肽的核酸。在一些实施方案中，一种或更多种靶核酸为内源植物核酸。在一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，MORC6样蛋白减少一种或更多种靶核酸的表达。在可与前述实施方案的任一个组合的一些实施方案中，MORC6样蛋白使一种或更多种靶核酸的表达沉默。
本公开内容还涉及用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含SUVR2多肽或其片段；以及b)在其中由所述重组核酸编码的重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。在一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含3个C2H2锌指结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含选自双螺旋束结构域、SRA结构域、pre-SET结构域或SET结构域的结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:66至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:66 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽与RNA聚合酶相互作用。在可与前述实施方案的任一个组合的一些实施方案中，RNA聚合酶为RNA聚合酶V。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽引起RNA指导的DNA甲基化。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为内源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸的表达被沉默。
本公开内容还涉及编码包含第一氨基酸序列和第二氨基酸序列的SUVR2样蛋白的重组核酸，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含SUVR2多肽或其片段。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:66至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:66 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在一些实施方案中，一种或更多种靶核酸为编码多肽的核酸。在一些实施方案中，一种或更多种靶核酸为内源植物核酸。在一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，SUVR2样蛋白减少一种或更多种靶核酸的表达。在可与前述实施方案的任一个组合的一些实施方案中，SUVR2样蛋白使一种或更多种靶核酸的表达沉默。
本公开内容的其他方面涉及包含重组核酸的载体，所述重组核酸编码前述实施方案的任一个的DMS3样蛋白、MORC6样蛋白和/或SUVR2样蛋白，其中所述重组核酸被可操作地连接至调节序列。本公开内容的其他方面涉及包含前述实施方案的表达载体的宿主细胞。在某些实施方案中，宿主细胞为植物细胞。本公开内容的其他方面涉及包含前述实施方案的任一个的重组核酸的重组植物。
本公开内容还涉及用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：(a)提供包含第一重组核酸和一种或更多种另外的重组核酸的植物，所述第一重组核酸编码包含第一氨基酸序列和第二氨基酸序列的第一重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含SHH1多肽或其片段，所述一种或更多种另外的重组核酸编码一种或更多种另外的多肽，所述一种或更多种另外的多肽的每种包含第一氨基酸序列和第二氨基酸序列，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含选自由SUVH2多肽或其片段、SUVH9多肽或其片段、DMS3多肽或其片段、MORC6多肽或其片段、SUVR2多肽或其片段、DRD1多肽或其片段、RDM1多肽或其片段、DRM3多肽或其片段、DRM2多肽或其片段和FRG多肽或其片段组成的组的多肽以及；以及b)在其中由所述第一重组核酸编码的第一重组多肽和由所述一种或更多种另外的重组核酸编码的一种或更多种另外的多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。在一些实施方案中，重组多肽中的至少一个引起RNA指导的DNA甲基化。在一些实施方案中，一种或更多种靶核酸为内源核酸。在一些实施方案中，一种或更多种靶核酸为异源核酸。在一些实施方案中，一种或更多种靶核酸的表达被沉默。
本公开内容的其他方面涉及包含表达载体的宿主细胞，所述表达载体包含编码前述实施方案的任一个的重组多肽的重组核酸。在一些实施方案中，宿主细胞为植物细胞。本公开内容的其他方面涉及包含前述实施方案的任一个的重组核酸的重组植物。
本公开内容还涉及用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括提供植物，所述植物包含：(a)靶向一种或更多种靶核酸的小干扰RNA(siRNA)，和编码一种或更多种多肽的一种或更多种重组核酸，所述一种或更多种多肽的每种包含第一氨基酸序列和第二氨基酸序列，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含选自由SUVH2多肽或其片段、SUVH9多肽或其片段、DMS3多肽或其片段、MORC6多肽或其片段、SUVR2多肽或其片段、DRD1多肽或其片段、RDM1多肽或其片段、DRM3多肽或其片段、DRM2多肽或其片段、和FRG多肽或其片段组成的组的多肽，以及b)在siRNA藉以与一种或更多种靶核酸相互作用并且由所述一种或更多种重组核酸编码的一种或更多种多肽藉以被表达的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。
本公开内容还涉及用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含DRD1多肽或其片段；以及b)在其中由所述重组核酸编码的重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。在一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含3个C2H2锌指结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含选自双螺旋束结构域、SRA结构域、pre-SET结构域或SET结构域的结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:79至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:79 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽与RNA聚合酶相互作用。在可与前述实施方案的任一个组合的一些实施方案中，RNA聚合酶为RNA聚合酶V。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽引起RNA指导的DNA甲基化。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为内源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸的表达被沉默。
本公开内容还涉及编码包含第一氨基酸序列和第二氨基酸序列的DRD1样蛋白的重组核酸，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含DRD1多肽或其片段。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:79至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:79 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在一些实施方案中，一种或更多种靶核酸为编码多肽的核酸。在一些实施方案中，一种或更多种靶核酸为内源植物核酸。在一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，DRD1样蛋白减少一种或更多种靶核酸的表达。在可与前述实施方案的任一个组合的一些实施方案中，DRD1样蛋白使一种或更多种靶核酸的表达沉默。
本公开内容还涉及用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含RDM1多肽或其片段；以及b)在其中由所述重组核酸编码的重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。在一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含3个C2H2锌指结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含选自双螺旋束结构域、SRA结构域、pre-SET结构域或SET结构域的结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:91至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:91 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽与RNA聚合酶相互作用。在可与前述实施方案的任一个组合的一些实施方案中，RNA聚合酶为RNA聚合酶V。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽引起RNA指导的DNA甲基化。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为内源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸的表达被沉默。
本公开内容还涉及编码包含第一氨基酸序列和第二氨基酸序列的RDM1样蛋白的重组核酸，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含RDM1多肽或其片段。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:91至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:91 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在一些实施方案中，一种或更多种靶核酸为编码多肽的核酸。在一些实施方案中，一种或更多种靶核酸为内源植物核酸。在一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，RDM1样蛋白减少一种或更多种靶核酸的表达。在可与前述实施方案的任一个组合的一些实施方案中，RDM1样蛋白使一种或更多种靶核酸的表达沉默。
本公开内容还涉及用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含DRM3多肽或其片段；以及b)在其中由所述重组核酸编码的重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。在一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含3个C2H2锌指结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含选自双螺旋束结构域、SRA结构域、pre-SET结构域或SET结构域的结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:101至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:101 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽与RNA聚合酶相互作用。在可与前述实施方案的任一个组合的一些实施方案中，RNA聚合酶为RNA聚合酶V。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽引起RNA指导的DNA甲基化。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为内源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸的表达被沉默。
本公开内容还涉及编码包含第一氨基酸序列和第二氨基酸序列的DRM3样蛋白的重组核酸，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含DRM3多肽或其片段。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:101至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:101 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在一些实施方案中，一种或更多种靶核酸为编码多肽的核酸。在一些实施方案中，一种或更多种靶核酸为内源植物核酸。在一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，DRM3样蛋白减少一种或更多种靶核酸的表达。在可与前述实施方案的任一个组合的一些实施方案中，DRM3样蛋白使一种或更多种靶核酸的表达沉默。
本公开内容还涉及用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含DRM2多肽或其片段；以及b)在其中由所述重组核酸编码的重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。在一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含3个C2H2锌指结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含选自双螺旋束结构域、SRA结构域、pre-SET结构域或SET结构域的结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:113至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:113 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽与RNA聚合酶相互作用。在可与前述实施方案的任一个组合的一些实施方案中，RNA聚合酶为RNA聚合酶V。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽引起RNA指导的DNA甲基化。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为内源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸的表达被沉默。
本公开内容还涉及编码包含第一氨基酸序列和第二氨基酸序列的DRM2样蛋白的重组核酸，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含DRM2多肽或其片段。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:113至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:113 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在一些实施方案中，一种或更多种靶核酸为编码多肽的核酸。在一些实施方案中，一种或更多种靶核酸为内源植物核酸。在一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，DRM2样蛋白减少一种或更多种靶核酸的表达。在可与前述实施方案的任一个组合的一些实施方案中，DRM2样蛋白使一种或更多种靶核酸的表达沉默。
本公开内容还涉及用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含FRG多肽或其片段；以及b)在其中由所述重组核酸编码的重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。在一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含3个C2H2锌指结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含选自双螺旋束结构域、SRA结构域、pre-SET结构域或SET结构域的结构域。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:125至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，第二氨基酸序列包含与SEQ ID NO:125 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽与RNA聚合酶相互作用。在可与前述实施方案的任一个组合的一些实施方案中，RNA聚合酶为RNA聚合酶V。在可与前述实施方案的任一个组合的一些实施方案中，重组多肽引起RNA指导的DNA甲基化。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为内源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，一种或更多种靶核酸的表达被沉默。
本公开内容还涉及编码包含第一氨基酸序列和第二氨基酸序列的FRG样蛋白的重组核酸，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含FRG多肽或其片段。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:125至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。在一些实施方案中，第二氨基酸序列包含与SEQ ID NO:125 100％相同的氨基酸序列。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含锌指结构域。在一些实施方案中，锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。在一些实施方案中，锌指结构域为锌指阵列。在一些实施方案中，锌指结构域选自C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域选自TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域包含TAL效应子靶向结构域。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在可与前述实施方案的任一个组合的一些实施方案中，DNA结合结构域结合一种或更多种靶核酸。在一些实施方案中，一种或更多种靶核酸为编码多肽的核酸。在一些实施方案中，一种或更多种靶核酸为内源植物核酸。在一些实施方案中，一种或更多种靶核酸为异源核酸。在可与前述实施方案的任一个组合的一些实施方案中，FRG样蛋白减少一种或更多种靶核酸的表达。在可与前述实施方案的任一个组合的一些实施方案中，FRG样蛋白使一种或更多种靶核酸的表达沉默。
本公开内容的其他方面涉及根据前述实施方案的任一个的方法具有一种或更多种靶核酸的减少的表达的植物，以及前述实施方案的植物的后代植物。在一些实施方案中，后代植物具有一种或更多种靶核酸的减少的表达并且不包含本公开内容的重组核酸。
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图1A-图1G阐释了RdDM突变体中受影响的sRNA簇的表观遗传谱。图1A阐释了饼状图，显示所示的分类中24nt siRNA读段的丰度(共5,967,213个独特映射的读段)。图1B阐释了维恩图，显示每个基因型中减少的24nt siRNA簇与用于下游分析的亚类的近似关系。图1C阐释了饼状图，显示所示的亚类中受影响的siRNA簇的染色体分布(基于此前描述的近着丝粒异染色质和常染色质的定义)。图1D和图1E阐释了在图1B中所示的亚类针对不同RdDM突变体的siRNA和CHH甲基化模式的箱形图(*指示显著减少；P<1e-10Mann-Whitney U检验)。图1F阐释了metaplot，显示在受影响的siRNA簇(从sRNA簇的中点+/-5000bp)处CMT3和Pol-V的富集。图1G阐释了跨受影响的siRNA簇的Pol-V富集的热图。
图2A-图2B阐释了在界定的siRNA簇处的聚合酶IV水平。图2A阐释了在所示的遗传背景中跨界定的siRNA簇的Pol-IV富集的metaplot且图2B阐释了在所示的遗传背景中跨界定的siRNA簇的Pol-IV富集的热图。Metaplot和热图从siRNA簇的中点延伸+/-5000bp。
图3A-图3D阐释了SHH1 SAWADEE结构域识别H3K9甲基化并采取独特的串联Tudor结构域样折叠。图3A和图3B阐释了结合至如所示的修饰或未修饰的组蛋白肽的SAWADEE结构域的基于ITC的测量。列出了Kd值。图3C阐释了metaplot分析，显示跨所示的Pol-IVChIP-seq峰种类的H3K9me2富集。图3D阐释了以游离形式的SHH1 SAWADEE结构域的总体结构。两个串联Tudor结构域以品红(Tudor 1)和绿色(Tudor 2)显示。SAWADEE结构域的独特的锌结合基序显示为放大的球棒模型(ball-and-stick model)，突出显示金属配位的细节。来自结晶条件的结合的去垢剂分子4-环己基-1-丁基-β-D-麦芽糖苷(-4,Hampton Research)部分显示为黄色以棒展示。
图4A-图4J阐释了用于SHH1 SAWADEE结构域识别H3(1-15)K9me2肽的结构基础和体内(in vivo)功能分析结果。图4A阐释了H3(1-15)K9me2-SAWADEE复合体的总体结构。SAWADEE结构域显示为带状图且肽以棒展示。还显示了模拟的退火复合物，省略了结合的肽在1σ水平的电子密度图。Tudor结构域1和2分别显示为品红和绿色。图4B阐释了立体视图，突出显示SAWADEE结构域与结合的H3(1-15)K9me2肽之间的分子间相互作用的细节。分子间氢键相互作用由红色虚线标出。还显示了识别二甲基化的K9的芳香族笼(aromatic cage)。图4C阐释了SAWADEE结构域与结合的H3(1-15)K9me2肽之间的分子间相互作用的展示。图4D阐释了H3K9me2肽结合的SAWADEE结构域(Tudor1呈品红且Tudor 2呈绿色)与游离形式的SAWADEE结构域(呈银色)的叠加揭示肽结合后没有显著的总体构象变化。图4E阐释了未甲基化的K4插入由来自Tudor 1的Glu130和Asp141、以及来自Tudor 2的Leu201和Tyr212形成的窄袋，并与Glu130和Asp141形成两个氢键、以及静电相互作用。图4F阐释了由三个芳香族残基形成的芳香族笼识别二甲基化的K9的详细视图。图4G阐释了单甲基化的K4为SAWADEE结构域的K4结合袋所容许，如由H3(1-15)K4me1K9me1-SAWADEE复合物的晶体结构揭示的。另外的甲基破坏与Glu130的一个氢键并引入与其他残基的更多范德华接触。图4H和图4I阐释了箱形图，分别显示在野生型和shh1突变体以及用具有野生型SHH1序列或K9(F162AF165A和Y140A)或K4(D141A和Y212A)结合袋中的点突变的SHH1构建体转化的shh1突变体中的％CHH甲基化和siRNA水平。(*指示显著性减少；P<1e-10Mann-Whitney U检验)。图4J阐释了定量PCR结果，显示Pol-IV在野生型和SHH1突变体背景中的富集。蓝色条指示对输入物和肌动蛋白水平标准化之后两个生物学重复的平均值。黑色条指示标准偏差。
图5A-图5F阐释了SUVH2和SUVH9是Pol V染色质结合、转录、和产生DNA甲基化所需的。图5A阐释了IGN22和P6相对于肌动蛋白7并对哥伦比亚(WT)标准化的定量PCR(qPCR)。两个生物学重复的平均值+/-标准偏差(SD)。图5B阐释了来自Flag ChIP的IGN22和IGN5的qPCR，显示为NRPE1-Flag/WT和NRPE1-Flag/suvh2suvh9系中IP/输入物相对于肌动蛋白7的富集。两个生物学重复的平均值+/-SD。图5C阐释了NRPE1-Flag/WT或NRPE1-Flag/suvh2suvh9中在由Flag ChIP-seq确定的界定的NRPE1位点处的NRPE1富集的热图，以哥伦比亚中的Flag ChIP作为阴性对照。图5D阐释了在C中显示的NRPE1-Flag/WT和NRPE1-Flag/suvh2suvh9的位点处的NRPE1富集的箱形图(胡须(whisker)延伸至±1.5四分位差(IQR))。图5E阐释了在所有界定的NRPE1结合位点处的甲基化百分比(平均值+/-SD)，如由全基因组亚硫酸氢盐测序确定的。图5F阐释了WT、suvh2suvh9和suvh4suvh5suvh6中H3K9me1 ChIP-seq读段的密度，由在80个选择的常染色质位点处的平均组蛋白甲基化水平标准化。
图6A-图6E阐释了拴系的SUVH2吸引DNA甲基化并导致晚开花表型。图6A，上图阐释了具有在N末端紧邻HA标签之前插入的Zn指的SUVH2的图。图6A，下图阐释了FWA基因的示意图，显示两个小的和两个大的重复段(蓝色箭头)、由PCR扩增的区域(启动子和转录物)、转录的开始(红色箭头)和由Zn指结合的位点(由两个橙色箭头指示)。图6B阐释了并排生长的植物，以阐释fwa-4中ZF-SUVH2(T2植物)与fwa-4相比的早开花。图6C阐释了哥伦比亚(WT)、fwa-4中ZF-SUVH2、fwa-4中HA-SUVH2和fwa-4的开花时间。平均值+/-SD。图6D阐释了FWA重复区域中在每个C处的甲基化百分比。数字指示单个C残基，两条黑色线显示ZF结合位点的位置，红色箭头显示转录物的位置。甲基化百分比从亚硫酸氢盐处理的DNA的20-25个克隆确定。图6E阐释了来自三个单独的T1植物的FWA重复区域中在每个C处的甲基化百分比。
图7A-图7C阐释了ZF-SUVH2将Pol V而非组蛋白甲基化募集至FWA。图7A阐释了WT(阳性对照)、nrpe1突变体(阴性对照)、fwa-4表观等位基因、和ZF-SUVH2/fwa-4中的NRPE1ChIP。两个表征的NRPE位点(IGN5和IGN22)和FWA中的两个区域(FWAp-启动子和FWAt-转录物)的qPCR显示为IP/输入物相对于阴性对照的富集。两个生物学重复的平均值+/-SD。图7B阐释了WT、fwa-4和ZF-SUVH2/fwa-4 T1花中的H3K9me1 ChIP。阴性对照是常染色质中缺乏DNA甲基化的区域。富集是相对于异染色质逆转录转座子Ta3。两个生物学重复的平均值+/-SD。图7C阐释了与图7B相同的内容，但ZF-SUVH2/fwa-4来自T2花。
图8A-图8H阐释了处于游离状态的SUVH9和结构域之间相互作用的晶体结构。图8A阐释了用于结晶的全长SUVH9和N末端截短的构建体的颜色编码示意图。图8B阐释了SUVH9的晶体结构的带状图，包含分别以粉色、绿色、橙色和蓝色显示的朝向N末端的双螺旋束、SRA结构域、pre-SET结构域和SET结构域。无序区域以虚线显示。Zn3Cys9簇(图的右下)以球棒模型突出显示。图8C阐释了旋转180°的两个替代视图中显示的双螺旋束内的疏水性相互作用和电荷相互作用。参与螺旋间疏水性相互作用的残基以黄色突出显示。图8D阐释了第一α-螺旋的N末端部分与SRA结构域和SET结构域形成电荷和氢键相互作用。相互作用的残基以棒展示显示且氢键相互作用以红色虚线显示。图8E阐释了第一α-螺旋的C末端部分展现与SRA结构域和pre-SET/SET结构域的广泛疏水性相互作用。来自SET结构域的长环的尖端覆盖在第一α-螺旋上并与之形成疏水性相互作用。相互作用的残基以棒展示显示。图8F阐释了第二α-螺旋与SRA结构域形成一些相互作用。相互作用的残基以棒展示显示且氢键相互作用以红色虚线显示。图8G阐释了SRA结构域形成与pre-SET/SET结构域和双螺旋束相互作用的疏水性核。相互作用的残基以棒展示显示。图8H阐释了SUVH9 SET结构域的长插入环(以品红突出显示)富含疏水性残基并与双螺旋束、pre-SET和SET结构域形成广泛疏水性相互作用。
图9A-图9E阐释了SAH-和肽-结合的GLP和以游离状态的SUVH9的结构比较和对于SUVH9缺少甲基转移酶活性的潜在结构基础。图9A阐释了人类GLP与结合的SAH(PDB编码:2IGQ)复合的晶体结构在上图中以银色带展示来显示，其SAH结合位点的展开视图在下图中以静电表面展示(electrostatic surface representataion)显示。辅因子SAH在上图和下图中以空间-填充展示来显示。GLP的SAH结合袋是相对窄的并以结构和形状互补结合SAH。图9B阐释了处于游离状态的SUVH9的晶体结构在上图中以彩色编码带展示来显示，推测的SAH结合位点的展开视图在下图中以静电表面展示来显示。SUVH9的推测的SAH结合袋是相对开放的和不会提供SAH分子的良好配合。图9C阐释了人类GLP与SAH和H3K9me2肽(PDB编码：2RFI)复合的晶体结构在上图中以银色带展示来显示，其肽结合位点的展开视图在下图中以静电表面展示来显示。参与肽底物结合的post-SET结构域和SET结构域的酸性环分别以蓝绿色和深蓝色突出显示。结合的肽在上图和下图中以空间-填充展示来显示。肽被结合在post-SET和SET结构域之间的表面裂缝中。图9D阐释了处于游离状态的SUVH9的晶体结构在上图中以彩色编码带展示来显示，推测的肽-结合位点的展开视图在下图中以静电表面展示来显示。SET结构域的长插入环以品红突出显示。推测的肽-结合位点被长插入环部分地封闭且蛋白表面上没有显著的肽-结合裂缝。图9E阐释了在叠加SUVH5 SRA-mCHH复合体[PDB编码：3Q0F)和处于游离状态的SUVH9的结构之后，将mCHHDNA放置到SUVH9 SRA结构域的活性位点的模型。DNA良好地配合到SRA结构域中而没有显著的空间位阻。在双螺旋束的第二α-螺旋上的一些周围残基以棒展示突出显示，所述残基可潜在地参与对DNA的结合。
图10阐释了拟南芥(Arabidopsis thaliana)SHH1、SHH2和SAWADEE结构域与来自其他物种包括蓖麻(Ricinus communis)、葡萄(Vitis vinifera)、北美云杉(Piceasitchensis)和玉蜀黍(Zea mays)中的SHH1直系同源物的基于结构的序列比对。还包括了人类UHRF1的串联Tudor结构域用于比较。SHH1 SAWADEE结构域和人类UHRF1串联Tudor结构域的二级结构分别展示在比对的上方和下方。SAWADEE结构域的参与结合去垢剂的非极性尾部的部分保守的疏水性残基以绿色星号标出。参与识别甲基化的K9的保守的芳香族残基以紫色六边形标出。参与配位锌离子的保守的SAWADEE残基以黑色圆圈标出。
图11阐释了来自拟南芥的SUVH家族蛋白的基于结构的序列比对。SUVH9的二级结构元件在序列比对的上方标出。结构域边界在上方标出。参与与翻转的5mC碱基相互作用的保守残基以及从公开的SUVH5-DNA复合物结构可得的DNA骨架分别以蓝绿色圆圈和蓝色六边形突出显示。SET结构域中的插入物以紫色框突出显示。锌配位的Cys残基在SET结构域中以黑色星号且在post-SET结构域中以灰色星号突出显示。保守并对于酶活性通常重要的两个酪氨酸残基以红色点突出显示。
图12a-图12c阐释了SUVH2和SUVH9全基因组地冗余作用。图12a阐释了跨各种SUVH突变体中鉴定的DMR的CHH甲基化的metaplot。图12b阐释了跨Pol V结合位点的CHH甲基化的metaplot。图12c阐释了维恩图，详述SUVH突变体中CHH低-甲基化的区域之间的重叠。
图13a-图13e阐释了Pol V结合依赖于DNA甲基化。图13a阐释了在所有界定的NRPE1结合位点处的CHH甲基化百分比的metaplot，如由野生型(WT)、nrpe1和suvh2 suvh9中的BS-seq确定的。图13b阐释了箱形图，显示WT和met1中在界定的NRPE1结合位点处每个胞嘧啶环境中的DNA甲基化。图13c阐释了met1中的Pol V占据在NRPE1位点处减少。图13d阐释了met1中界定为高-甲基化的位点处的DNA甲基化的metaplot。图13e阐释了met1中Pol V占据在界定的高-甲基化的位点处增加。
图14A-图14B阐释了拴系的SUVH2通过DRD1募集Pol V，导致DNA甲基化和晚开花表型。图14A阐释了与T2 ZF-KYP/fwa-4(未甲基化的)和WT(标准甲基化模式)相比，在FWA重复区域中的每个胞嘧啶处的甲基化百分比，如由T2和T3 ZF-SUVH2/fwa-4植物中BS-seq确定的。ZF结合位点显示为绿色且FWA基因显示为蓝色。图14B阐释了以ZF-SUVH2拉下DRD1-Flag的结果。输入物:来自拟南芥的DRD1-Flag提取物；珠模拟物：从使用预结合本氏烟草(Nicotiana benthamiana)提取物的HA磁珠的DRD1-Flag拉下洗脱；珠-ZFSUVH2：从使用预结合本氏烟草ZF-SUVH2提取物的HA-磁珠的DRD1-Flag拉下洗脱。上图：Flag印迹；下图：HA印迹。
图15A-图15C阐释了ZF-SUVH2构建体通过与DRD1直接相互作用将Pol V稳定地募集至FWA。图15A阐释了WT相比于ZF-KYP(加flag标签的)中的Flag-ChIP，显示在启动子和转录物区域二者中在FWA处的富集。图15B阐释了来自含有ZF-SUVH2转基因的Basta-抗性系和已经丢失ZF-SUVH2转基因的两个Basta-敏感性姊妹株的FWA的BS-Seq。图15C阐释了利用Flag-DRD1拟南芥中HA-SUVH2的拉下。左图是来自两个亲本株系(表达HA-SUVH2(HA-2)或Flag-DRD1(Flag-D))和表达HA-SUVH2和Flag DRD1(HA-2xFlag-d)二者的F2系的输入物。右图显示洗脱Flag-磁珠。上图是HA印迹，下图是Flag印迹。
图16阐释了由DRD1免疫沉淀-质谱鉴定的蛋白列表。
图17阐释了野生型、fwa-4和DMS3-ZF和MORC6-ZF早开花系中在FWA基因处的CG、CHG和CHH甲基化。红色代表CHH甲基化，其中H是C、A或T。绿色代表CG甲基化。蓝色代表CHG甲基化。浅蓝色代表与polV突变体相比，野生型中的Pol V结合，显示PolV结合至野生型植物中FWA的启动子区域。FWA基因的示意图在下方以深蓝色展示。
图18阐释了野生型植物(上半部分)或含有TAL-SHH1的植物(下半部分)中在APETALA1(At1g69120)启动子区域处存在的小RNA。在上方链上的siRNA为红色且在下方链上的siRNA为蓝色。
图19阐释了仅包含TAL-SUVH2、TAL-SHH1或包含TAL-SHH1和TAL-SUVH2二者(TAL-SHH1/TAL-SUVH2)的F1植物中在SUPERMAN基因(At3g23130)启动子区域处的CHH DNA甲基化。
详述
综述
提出以下描述是为了使本领域普通技术人员能够制造和使用多个实施方案。仅作为实例提供具体的装置、技术、方法和应用的描述。对本文记载的实例的多种改变对本领域普通技术人员是显而易见的，并且本文定义的一般性原则可以应用于其他实例和应用中，而不会与多个实施方案的精神和范围相背离。因此，不意欲将多个实施方案限制为本文记载和显示的实例。
本公开内容涉及引起表观遗传基因沉默的重组蛋白，和使用所述蛋白用于降低植物中基因表达的方法。
在拟南芥(Arabidopsis)中，DNA甲基化由被称为DRM2的蛋白质确立，并被24nt的小干扰RNA(siRNA)通过称为RNA指导的DNA甲基化(RdDM)的途径靶向，所述途径包括两个植物特异性的RNA聚合酶：RNA聚合酶IV(Pol IV)，其功能是起始siRNA的生物合成；和RNA聚合酶V(Pol V)，其功能是在RdDM途径的下游DNA甲基转移酶靶向阶段产生募集下游RdDM因子的非编码支架转录物。因此，拟南芥中的RNA指导的DNA甲基化(RdDM)包括通过Pol IV的非编码小干扰RNA的合成和通过Pol V的非编码支架RNA的合成二者。
本公开内容(至少部分)基于申请人的发现：称为SHH1的蛋白在RdDM途径中起作用以使能够从RdDM靶产生siRNA，和SHH1是RNA聚合酶IV(Pol IV)占据在这些靶基因座基因座处所必需的。本公开内容还(至少部分)基于申请人的发现：Pol V与染色质的缔合依赖于被称为SUVH2和SUVH9的两个蛋白。此外，可以通过将异源DNA结合结构域引入所述蛋白或所述蛋白的片段(例如异源性锌指结构域或TAL效应子靶向结构域)中，将修饰的SHH1、SUVH2和/或SUVH9蛋白工程化，以特异性结合不同的DNA序列。有利的是，所述重组蛋白可用于将PolIV(用于SHH1样蛋白)或Pol V(用于SUVH2样蛋白和/或SUVH9样蛋白)募集到靶基因座，以在靶基因座处诱导RNA指导的DNA甲基化，并因此沉默所述靶基因座。
在本公开内容的方法中有用的用于将Pol V、DNA甲基化和基因沉默靶向到特定基因座的其他蛋白包括修饰的的DMS3、MORC6和/或SUVR2蛋白的任何一个，该其他蛋白也可以通过将异源DNA结合结构域引入所述蛋白或所述蛋白的片段(例如异源性锌指结构域或TAL效应子靶向结构域)中，而被工程化，以特异性结合不同的DNA序列。
因此，本公开内容提供了使用SHH1蛋白、SUVH2蛋白、SUVH9蛋白、DMS3蛋白、MORC6蛋白和/或SUVR2蛋白中的一种或更多种沉默植物中的特定基因座的方法，所述SHH1蛋白、SUVH2蛋白、SUVH9蛋白、DMS3蛋白、MORC6蛋白和/或SUVR2蛋白已经通过将异源DNA结合结构域引入所述蛋白而被工程化，以特异性结合不同的DNA序列。前述修饰的蛋白中的每一个可以单独或以不同组合方式在宿主细胞中表达，以起作用来沉默靶基因座。例如，具有异源DNA结合结构域的修饰的SHH1蛋白可以在宿主细胞中表达以将Pol IV靶向至靶基因座，联合具有异源DNA结合结构域的修饰的SUVH2蛋白、SUVH9蛋白、DMS3蛋白、MORC6蛋白和/或SUVR2蛋白中的一种或更多种将Pol V靶向至所述相同的靶基因座，以触发所述靶基因座的RNA指导的DNA甲基化和外遗传沉默。
可用于根据本公开内容的方法沉默植物中特定基因座的其他蛋白包括另外的参与RNA指导的DNA甲基化的蛋白。例如，适合用于本公开内容的方法中的其他蛋白可以包括例如DRD1、RDM1、DRM3、DRM2和FRG中的任意一个。因此，本公开内容还提供了使用SHH1蛋白、SUVH2蛋白、SUVH9蛋白、DMS3蛋白、MORC6蛋白、SUVR2蛋白、DRD1蛋白、RDM1蛋白、DRM3蛋白、DRM2蛋白和/或FRG蛋白中的一种或更多种沉默植物中的特定基因座的方法，所述SHH1蛋白、SUVH2蛋白、SUVH9蛋白、DMS3蛋白、MORC6蛋白、SUVR2蛋白、DRD1蛋白、RDM1蛋白、DRM3蛋白、DRM2蛋白和/或FRG蛋白已经通过将异源DNA结合结构域引入所述蛋白而被工程化，以特异性结合不同的DNA序列。前述修饰的蛋白中的每一个可以单独或以不同组合方式在宿主细胞中表达，以起作用来沉默靶基因座。
本公开内容的用于沉默宿主细胞中靶基因座的方法还可包括在靶基因座引入小干扰RNA(siRNA)，联合Pol V通过SUVH2蛋白、SUVH9蛋白、DMS3蛋白、MORC6蛋白、SUVR2蛋白、DRD1蛋白、RDM1蛋白、DRM3蛋白、DRM2蛋白和/或FRG蛋白中的一种或更多种靶向所述基因座。产生siRNA的方法是本领域熟知的。这些方法包括例如在细胞中被天然加工为小干扰RNA的发夹RNA的表达。制造小干扰RNA的发夹构建体是本领域已知的(EMBO Reports,2006Nov；7(11):1168-75)。另外的用于产生siRNA的方法包括例如将来自外源性来源的小干扰RNA直接引入细胞中。描述将siRNA轰击到植物中的方法是本领域已知的(Science 328,912(2010))。RNA分子还可以被喷洒(外源应用)到植物上以使得此后小RNA可以在植物细胞中产生(参见美国专利申请2014/0018241)。因此，本公开内容的用于沉默宿主细胞中靶基因座的方法还可包括在靶基因座引入小干扰RNA(siRNA)，联合Pol V通过含有异源DNA结合结构域的SUVH2蛋白、SUVH9蛋白、DMS3蛋白、MORC6蛋白、SUVR2蛋白、DRD1蛋白、RDM1蛋白、DRM3蛋白、DRM2蛋白和/或FRG蛋白中的一种或更多种靶向所述基因座。
通过靶向本公开内容的不同重组蛋白(诸如，例如SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白)引起的沉默甚至在不存在这些重组蛋白的情况下也可在植物中稳定。因此，本公开内容的方法可以允许植物中的一种或更多种靶核酸在本公开内容的重组多核苷酸已经被从所述植物中除去之后保持沉默。例如，在siRNA和本公开内容的募集Pol V的重组蛋白一起靶向特定区域之后，所述靶区域的沉默和DNA甲基化甚至在除去该转基因之后可以保持稳定。本公开内容的目的是提供根据本公开内容的方法的具有降低的表达的一种或更多种靶核酸的植物。由于本公开内容的方法可以允许植物中的一种或更多种靶核酸在本公开内容的重组多核苷酸已经被从所述植物中除去之后保持沉默，这些植物的后代植物甚至在不存在产生本公开内容的重组多肽的重组多核苷酸的情况下可以具有降低的表达的一种或更多种靶核酸。
定义
除非另有限定，所有科学和技术术语应理解为具有与它们所属领域中通常所用相同的含义。为了本公开内容的目的，定义了以下术语。
如本文所用的，“靶核酸”是指有利地与蛋白质结合的双链多核苷酸的一部分，所述双链多核苷酸例如RNA、DNA、PNA(肽核酸)或其组合。在一个实施方案中，“靶核酸”是一个基因的转录调控元件的全部或部分，所述基因的所需表型结果可以通过改变其表达的程度而获得。转录调控元件包括正调控元件和负调控元件例如启动子、增强子、其他应答元件，阻抑蛋白结合位点、操纵基因和/或沉默基因，所述其他应答元件例如类固醇应答元件、热激应答元件、金属应答元件。转录调控元件可以是病毒的、真核的或原核的。“靶核酸”还包括编码区域或蛋白编码基因的全部或部分。“靶核酸”还包括可结合蛋白并且其表达从而被调节(通常阻止转录)的下游核酸。
如本文所用的，“靶基因”是指其表达被蛋白质降低的基因，所述蛋白质诸如，例如SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白。
如本文所用的，术语“多核苷酸”、“核酸”、“核酸序列”、“核酸的序列”和其变化应该与以下术语是通用的：多脱氧核糖核苷酸(包括2-脱氧-D-核糖)、多核糖核苷酸(包括D-核糖)、任何其他类型的为嘌呤或嘧啶碱基的N-糖苷的多核苷酸，和其他包含非核苷酸骨架的聚合物，条件是所述聚合物包含呈允许见于DNA和RNA中的碱基配对和碱基堆叠(basestack)的构象的核碱基。因此，这些术语包括已知类型的核酸序列修饰，例如用类似物置换一种或更多种天然存在的核苷酸；核苷酸间的修饰，诸如，例如具有不带电荷的连接(例如甲基膦酸酯类、磷酸三酯类、氨基磷酸酯类、氨基甲酸酯类等)的那些，具有带负电荷的连接(例如硫代磷酸酯类、二硫代磷酸酯类等)的那些，和具有带正电荷的连接(例如氨基烷基氨基磷酸酯类、氨基烷基磷酸三酯类)的那些；包含附连部分(pendant moieties)的那些，所述附连部分诸如，例如蛋白质(包括核酸酶、毒素、抗体、信号肽、聚L-赖氨酸等)；具有嵌入剂(例如吖啶、补骨脂素等)的那些；和包含螯合剂(例如金属、放射性金属、硼、氧化性金属等)的那些。如本文所用的，用于核苷酸和多核苷酸的符号是IUPAC-IUB生物化学命名法委员会推荐的那些(Biochem.9,4022(1970)。
如本文所用的，“多肽”是包含多个连续聚合的氨基酸残基(例如，可选择地，至少约15个连续聚合的氨基酸残基，至少约30个连续聚合的氨基酸残基，或至少约50个连续聚合的氨基酸残基)的氨基酸序列。在许多情况下，多肽包含聚合的氨基酸残基序列，所述聚合的氨基酸残基序列为酶、甲基转移酶、脱甲基酶、脱乙酰酶、具有未知功能的预测蛋白、或者其结构域或部分或片段。多肽可选择地包含修饰的氨基酸残基、不是由密码子编码的天然存在的氨基酸残基和非天然存在的氨基酸残基。
如本文所用的，“蛋白质”是指天然存在的或合成的氨基酸序列、寡肽、肽、多肽或其部分。
可用于本公开内容的基因和蛋白质包括编码保守性修饰变体的基因，和为遍及本申请所记载的那些基因和蛋白质的保守性修饰变体的蛋白质。本文使用的“保守性修饰变体”包括导致氨基酸被化学性质类似的氨基酸置换的对多肽序列的单个置换、缺失或添加。提供功能上类似的氨基酸的保守性置换表是本领域熟知的。这类保守性修饰变体是对本公开内容的多态性变体、种间同源物和等位基因的补充，并且不将本公开内容的多态性变体、种间同源物和等位基因的补充排除在外。以下八组包含相互间为保守性置换的氨基酸：1)丙氨酸(A)、甘氨酸(G)；2)天冬氨酸(D)、谷氨酸(E)；3)天冬酰胺(N)、谷氨酰胺(Q)；4)精氨酸(R)、赖氨酸(K)；5)异亮氨酸(I)、亮氨酸(L)、甲硫氨酸(M)、缬氨酸(V)；6)苯丙氨酸(F)、酪氨酸(Y)、色氨酸(W)；7)丝氨酸(S)、苏氨酸(T)；和8)半胱氨酸(C)、甲硫氨酸(M)(参见，例如Creighton,Proteins(1984))。
本文记载的基因和蛋白的同源物也可用于本公开内容中。如本文所用的，“同源性”是指参考序列与第二序列的至少一个片段之间的序列相似性。可以通过任何本领域已知的方法鉴定同源物，优选通过使用BLAST工具以将参考序列与单个第二序列或序列片段或序列数据库比对。如下文所述，BLAST基于同一性和相似性百分比比对序列。如本文所用的，“直系同源”是指不同物种中的衍生自一个共同祖先基因的基因。
在两个或更多核酸或多肽序列的上下文中，术语“相同的”或“同一性”百分比是指相同的两个或更多序列或子序列。当使用以下序列比较算法中的一个或通过人工比对和目视检查进行测量，比较并比对一个比较窗口或指定区域中的最大一致性时，如果两个序列具有指定百分比的相同的氨基酸残基或核苷酸(即在指定区域上或者当未指定时在整条序列上具有29％同一性，可选择地30％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％、99％或100％同一性)，那么两个序列“基本上相同”。可选择地，同一性存在于长度为至少约50个核苷酸(或10个氨基酸)的区域，或者更优选长度为100至500或1000或更多个核苷酸(或20、50、200或更多个氨基酸)的区域。
对于序列比较，通常一个序列充当参考序列，测试序列与所述参考序列比较。当使用序列比较算法时，将测试序列和参考序列输入计算机中，如果需要的话指定子序列坐标，并指定序列算法程序参数。可以使用默认程序参数，或者可以指定替代性参数。然后序列比较算法基于程序参数，计算测试序列相对于参考序列的序列同一性百分比。当比较两个序列的一致性时，不需要序列是连续的，但是任何空位会为其产生罚分，所述罚分会降低整体一致性百分比。对于blastn，默认参数是空位开口罚分＝5，空位延伸罚分＝2。对于blastp，默认参数是空位开口罚分＝11，空位延伸罚分＝1。
如本文所用的，“比较窗口”包括以下含义：具有任一数目的连续位置的区段，所述数量包括但不限于从20至600，通常约50至约200，更通常约100至约150，在所述区段中，序列可以与相同数目的连续位置的参考序列在将这两个序列最佳比对之后进行比较。用于比较的序列比对方法是本领域熟知的。用于比较的最佳序列比对可以例如通过Smith和Waterman(1981)的本地同源性算法、通过Needleman和Wunsch(1970)J Mol Biol 48(3):443-453的同源性比对算法、通过Pearson和Lipman(1988)Proc Natl Acad Sci USA 85(8):2444-2448的寻找相似性方法，通过这些算法的计算机化工具(Wisconsin遗传软件包中的GAP、BESTFIT、FASTA和TFASTA,Genetics Computer Group,575 Science Dr.,Madison,WI)，或者通过人工比对和目视检查[参见，例如，Brent等人,(2003)CurrentProtocols in Molecular Biology,John Wiley&Sons,Inc.(Ringbou编辑)]进行。
适用于确定序列同一性百分比和序列相似性的算法的两个实例是BLAST和BLAST2.0算法，这两个算法分别记载于Altschul等人(1997)Nucleic Acids Res 25(17):3389-3402和Altschul等人(1990)J.Mol Biol 215(3)-403-410中。用于进行BLAST分析的软件是公众通过国家生物技术信息中心可得的。该算法包括首先通过识别查询序列中具有长度W的短字段而识别高得分序列对(HSP)，所述短字段在当所述短字段与数据库序列中的具有相同长度的字段比对时，匹配或满足一些正值阈值得分(positive-valued thresholdscore)T。T被称为邻字段得分阈值(neighborhood wood score threshold)(Altschul等人，如上所述)。这些最初邻字段命中(hit)作为用于起始找到包含它们的更长HSP的搜索的种子。只要累计比对得分可以增加，字段命中就沿着每个序列在两个方向延伸。对于核苷酸序列，使用参数M(对于一对匹配残基的奖励得分，一直>0)和N(对于错配残基的罚分，一直<0)计算累计得分。对于氨基酸序列，使用打分矩阵计算累计得分。当累计比对得分从其最大达到值下降了数量X时；由于一个或更多个负得分残基比对的积累而使累计致使得分为0或低于0时；或达到任一序列的末端时，字段命中在每个方向的延伸终止。BLAST算法参数W、T和X决定比对的灵敏度和速度。BLASTN程序(对于核苷酸序列)使用字段长度(W)＝11，期望值(E)＝10，M＝5，N＝5，和两条链的比较作为默认。对于氨基酸序列，BLASTP程序使用字段长度＝3，和期望值(E)＝10，和BLOSUM62打分矩阵(参见Henikoff和Henikoff,(1992)ProcNatl Acad Sci USA 89(22):10915-10919)比对(B)＝50，期望值(E)＝10，M＝5，N＝-4，以及两条链的比较作为默认。
BLAST算法还进行两个序列间的相似性的统计学分析(参见，例如Karlin和Altschul,(1993)Proc Natl Acad Sci USA 90(12):5873-5877)。一种由BLAST算法提供的相似性测量是最小总和可能性(P(N))，其提供两条核苷酸或氨基酸序列之间的匹配会随机出现的可能性的指示。例如，如果在测试核酸与参考核酸的比较中最小总和可能性小于约0.2，更优选小于约0.01，以及最优选小于约0.001，那么就认为核酸与参考序列相似。
除了上文所述的序列同一性百分比之外，两个核酸序列或多肽基本上相同的另外的指示是由第一核酸编码的多肽与针对由第二核酸编码的多肽所产生的抗体在免疫学上具有交叉反应性。因此，例如当两条肽的差异仅在于保守性置换时，一条多肽通常与第二多肽基本上相同。两条核酸序列基本上相同的另外的指示是两个分子或它们的互补序列在严格条件下相互杂交。两条核酸序列基本上相同的又一指示是可以使用相同引物扩增所述序列。
本公开内容的重组蛋白
本文提供了用于降低植物中靶核酸的表达的重组蛋白。在一些实施方案中，本公开内容的重组蛋白与RNA聚合酶相互作用。该相互作用可以是直接的，或者它可以是间接的。不论本公开内容的重组蛋白与RNA聚合酶的相互作用是直接的还是间接的，所述相互作用都促进RNA聚合酶募集到核酸。在一些实施方案中，一种或更多种另外的蛋白还可以参与促进本公开内容的重组蛋白与RNA聚合酶的相互作用以及RNA聚合酶募集到核酸。在一些实施方案中，SHH1样蛋白直接地或间接地与RNA Pol IV相互作用，并且该相互作用促进RNAPol IV募集到核酸。在一些实施方案中，SUVH2样蛋白和/或SUVH9样蛋白直接地或间接地与RNA Pol V相互作用，并且该相互作用促进RNA Pol V募集到核酸。在一些实施方案中，DMS3样蛋白、MORC6样蛋白和/或SUVR2样蛋白直接地或间接地与RNA Pol V相互作用，并且该相互作用促进RNA Pol V募集到核酸。在一些实施方案中，DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白直接地或间接地与RNA Pol V相互作用，并且该相互作用促进RNA Pol V募集到核酸。在一些实施方案中，本公开内容的重组蛋白促进核酸的RNA指导的DNA甲基化。
在一些实施方案中，SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白被靶向到同一核酸，并且协同作用以沉默靶核酸的表达。本公开内容的重组蛋白例如SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白可以单独或组合地在细胞中重组表达。
SHH1蛋白
本公开内容的某些方面涉及SHH1样蛋白。在一些实施方案中，SHH1样蛋白是指重组SHH1蛋白或其片段，以及包含异源DNA结合结构域的重组SHH1蛋白或其片段。SHH1样蛋白可以用于降低植物中一种或更多种靶核酸例如基因的表达。
本公开内容的SHH1蛋白是SAWADEE同源异型域同源物1(SAWADEE HOMEODOMAINHOMOLOG 1)(SHH1)蛋白。全长SHH1蛋白包含染色质结合SAWADEE结构域。SAWADEE染色质结合结构域采取独特的串联Tudor样折叠，并且起探查组氨酸3(H3)尾上的未甲基化的K4和甲基化的K9修饰的双赖氨酸读码器的作用。SHH1蛋白还包含同源异型域。在一些实施方案中，本公开内容的SHH1样蛋白是染色质结合蛋白。
在一些实施方案中，本公开内容的SHH1样蛋白包括全长SHH1蛋白的功能片段，其中所述片段保持将RNA Pol IV募集到DNA的能力。在一些实施方案中，SHH1蛋白片段包含全长SHH1蛋白的至少20个连续氨基酸、至少30个连续氨基酸、至少40个连续氨基酸、至少50个连续氨基酸、至少60个连续氨基酸、至少70个连续氨基酸、至少80个连续氨基酸、至少90个连续氨基酸、至少100个连续氨基酸、至少120个连续氨基酸、至少140个连续氨基酸、至少160个连续氨基酸、至少180个连续氨基酸、至少200个连续氨基酸、至少220个连续氨基酸、至少240个连续氨基酸、或241或更多个连续氨基酸。在一些实施方案中，SHH1蛋白片段可以包括从全长SHH1蛋白的连续氨基酸序列中除去了一个或更多个氨基酸的序列。在一些实施方案中，SHH1蛋白片段可以包括在全长SHH1蛋白的连续氨基酸序列中一个或更多个氨基酸被用与存在于给定氨基酸位点的内源性氨基酸不同的氨基酸替换/置换的序列。在一些实施方案中，SHH1蛋白片段可以包括一个或更多个氨基酸被添加到全长SHH1蛋白的另外的连续氨基酸序列中的序列。
可以从单子叶植物和双子叶植物中鉴定和分离合适的SHH1蛋白。这类植物的实例包括，例如，拟南芥属种(Arabidopsis spp.)、蓖麻(Ricinus communis)、大豆(Glycinemax)、玉蜀黍(Zea Mays)、蒺藜苜蓿(Medicago truncatula)、小立碗藓(Physcomitrellapatens)、双色蜀黍(Sorghum bicolor)和稻(Oryza sativa)。合适的SHH1蛋白的实例可包括，例如，表1中列出的那些、其同源物和其直系同源物。
表1：SHH1蛋白
在一些实施方案中，本公开内容的SHH1蛋白或其片段具有与拟南芥(A.thaliana)SHH1蛋白的氨基酸序列(即，SEQ ID NO:1)具有至少约20％、至少约25％、至少约30％、至少约40％、至少约50％、至少约55％、至少约60％、至少约65％、至少约70％、至少约75％、至少约80％、至少约85％、至少约90％、至少约91％、至少约92％、至少约93％、至少约94％、至少约95％、至少约96％、至少约97％、至少约98％、至少约99％、或至少约100％氨基酸同一性的氨基酸序列。
在一些实施方案中，SHH1蛋白的同源物或SHH1蛋白的片段的同源物是拟南芥SHH2蛋白的氨基酸序列(SEQ ID NO:40)。
SHH1样蛋白可以包括任何SHH1同源物或直系同源物，例如表1中列出的那些的任意一个，的氨基酸序列或其片段。技术人员会容易地认识到另外的SHH1同源物和/或直系同源物可以存在，并且可用于本文。
SUVH2蛋白和SUVH9蛋白
本公开内容的某些方面涉及SUVH2样蛋白和SHVU9样蛋白。在一些实施方案中，SUVH2样蛋白是指重组SUVH2蛋白或其片段，以及包含异源DNA结合结构域的重组SUVH2蛋白或其片段。在一些实施方案中，SUVH9样蛋白是指重组SUVH9蛋白或其片段，以及包含异源DNA结合结构域的重组SUVH9蛋白或其片段。SUVH2样蛋白和/或SUVH9样蛋白可以用于降低植物中一种或更多种靶核酸例如基因的表达。
本公开内容的SUVH2和SUVH9蛋白是SU-VAR(3-9)同源物。全长SUVH2和SUVH9蛋白包含一个朝向N末端的双螺旋束结构域、一个SRA结构域以及朝向C末端的pre-SET和SET结构域。SUVH结构域的结构和序列特征是本领域已知的，并且在本文中提供。在一些实施方案中，本公开内容的SUVH2样蛋白和/或SUVH9样蛋白可以包含标准的SUVH结构域的一个或更多个，所述SUVH结构域包括一个双螺旋束结构域、一个SRA结构域、一个pre-SET结构域和/或一个SET结构域。
在一些实施方案中，本公开内容的SUVH2样蛋白包括全长SUVH2蛋白的功能片段，其中所述片段保持将RNA Pol V募集到DNA的能力。在一些实施方案中，SUVH2蛋白片段包含全长SUVH2蛋白的至少20个连续氨基酸、至少30个连续氨基酸、至少40个连续氨基酸、至少50个连续氨基酸、至少60个连续氨基酸、至少70个连续氨基酸、至少80个连续氨基酸、至少90个连续氨基酸、至少100个连续氨基酸、至少120个连续氨基酸、至少140个连续氨基酸、至少160个连续氨基酸、至少180个连续氨基酸、至少200个连续氨基酸、至少220个连续氨基酸、至少240个连续氨基酸、至少260个连续氨基酸、至少280个连续氨基酸、至少300个连续氨基酸、至少325个连续氨基酸、至少350个连续氨基酸、至少375个连续氨基酸、至少400个连续氨基酸、至少425个连续氨基酸、至少450个连续氨基酸、至少475个连续氨基酸、至少500个连续氨基酸、至少525个连续氨基酸、至少550个连续氨基酸、至少575个连续氨基酸、至少600个连续氨基酸、至少625个连续氨基酸、或626或更多个连续氨基酸。在一些实施方案中，SUVH2蛋白片段可以包括从全长SUVH2蛋白的连续氨基酸序列中除去了一个或更多个氨基酸的序列。在一些实施方案中，SUVH2蛋白片段可以包括在全长SUVH2蛋白的连续氨基酸序列中一个或更多个氨基酸被用与存在于给定氨基酸位点的内源性氨基酸不同的氨基酸替换/置换的序列。在一些实施方案中，SUVH2蛋白片段可以包括一个或更多个氨基酸被添加到全长SUVH2蛋白的另外的连续氨基酸序列中的序列。
可以从单子叶植物和双子叶植物中鉴定和分离合适的SUVH2蛋白。这类植物的实例包括，例如，拟南芥属种、蓖麻、大豆、玉蜀黍、蒺藜苜蓿、小立碗藓、双色蜀黍和稻。合适的SUVH2蛋白的实例可包括，例如，表2中列出的那些、其同源物和其直系同源物。
表2：SUVH2蛋白
生物体 基因名 SEQ ID NO.
拟南芥 NP_180887.1 14
蓖麻 XP_002528332.1 15
大豆 XP_003530311.1 16
玉蜀黍 DAA60407.1 17
蒺藜苜蓿 XP_003619209.1 18
小立碗藓 XP_001753516.1 19
双色蜀黍 XP_002459773.1 20
稻 EAZ03669.1 21
二穗短柄草 XP_003563196.1 22
毛果杨 XP_002315593.1 23
葡萄 XP_002282386.1 24
黄瓜 XP_004134031.1 25
琴叶拟南芥 XP_002879445.1 26
在一些实施方案中，本公开内容的SUVH2蛋白或其片段具有与拟南芥SUVH2蛋白的氨基酸序列(即，SEQ ID NO:14)具有至少约20％、至少约25％、至少约30％、至少约40％、至少约50％、至少约55％、至少约60％、至少约65％、至少约70％、至少约75％、至少约80％、至少约85％、至少约90％、至少约91％、至少约92％、至少约93％、至少约94％、至少约95％、至少约96％、至少约97％、至少约98％、至少约99％、或至少约100％氨基酸同一性的氨基酸序列。
SUVH2样蛋白可以包括任何SUVH2同源物或直系同源物，例如表2中列出的那些的任意一个，的氨基酸序列或其片段。技术人员会容易地认识到另外的SUVH2同源物和/或直系同源物可以存在，并且可用于本文。
在一些实施方案中，本公开内容的SUVH9样蛋白包括全长SUVH9蛋白的功能片段，其中所述片段保持将RNA Pol V募集到DNA的能力。在一些实施方案中，SUVH9蛋白片段包含全长SUVH9蛋白的至少20个连续氨基酸、至少30个连续氨基酸、至少40个连续氨基酸、至少50个连续氨基酸、至少60个连续氨基酸、至少70个连续氨基酸、至少80个连续氨基酸、至少90个连续氨基酸、至少100个连续氨基酸、至少120个连续氨基酸、至少140个连续氨基酸、至少160个连续氨基酸、至少180个连续氨基酸、至少200个连续氨基酸、至少220个连续氨基酸、至少240个连续氨基酸、至少260个连续氨基酸、至少280个连续氨基酸、至少300个连续氨基酸、至少325个连续氨基酸、至少350个连续氨基酸、至少375个连续氨基酸、至少400个连续氨基酸、至少425个连续氨基酸、至少450个连续氨基酸、至少475个连续氨基酸、至少500个连续氨基酸、至少525个连续氨基酸、至少550个连续氨基酸、至少575个连续氨基酸、至少600个连续氨基酸、至少625个连续氨基酸、或626或更多个连续氨基酸。在一些实施方案中，SUVH9蛋白片段可以包括从全长SUVH9蛋白的连续氨基酸序列中除去了一个或更多个氨基酸的序列。在一些实施方案中，SUVH9蛋白片段可以包括在全长SUVH9蛋白的连续氨基酸序列中一个或更多个氨基酸被用与存在于给定氨基酸位点的内源性氨基酸不同的氨基酸替换/置换的序列。在一些实施方案中，SUVH9蛋白片段可以包括一个或更多个氨基酸被添加到全长SUVH9蛋白的另外的连续氨基酸序列中的序列。
可以从单子叶植物和双子叶植物中鉴定和分离合适的SUVH9蛋白。这类植物的实例包括，例如，拟南芥属种、蓖麻、大豆、玉蜀黍、蒺藜苜蓿、小立碗藓、双色蜀黍和稻。合适的SUVH9蛋白的实例可包括，例如，表3中列出的那些、其同源物和其直系同源物。
表3：SUVH9蛋白
在一些实施方案中，本公开内容的SUVH9蛋白或其片段具有与拟南芥SUVH9蛋白的氨基酸序列(即，SEQ ID NO:27)具有至少约20％、至少约25％、至少约30％、至少约40％、至少约50％、至少约55％、至少约60％、至少约65％、至少约70％、至少约75％、至少约80％、至少约85％、至少约90％、至少约91％、至少约92％、至少约93％、至少约94％、至少约95％、至少约96％、至少约97％、至少约98％、至少约99％、或至少约100％氨基酸同一性的氨基酸序列。
SUVH9样蛋白可以包括任何SUVH9同源物或直系同源物，例如表3中列出的那些的任意一个，的氨基酸序列或其片段。技术人员会容易地认识到另外的SUVH9同源物和/或直系同源物可以存在，并且可用于本文。
DMS3蛋白
本公开内容的某些方面涉及DMS3样蛋白。在一些实施方案中，DMS3样蛋白是指重组DMS3蛋白或其片段，以及包含异源DNA结合结构域的重组DMS3蛋白或其片段。DMS3样蛋白可以用于降低植物中一种或更多种靶核酸例如基因的表达。
DMS3蛋白是本领域已知的，并记载于本文中。在一些实施方案中，本公开内容的DMS3样蛋白包括全长DMS3蛋白的功能片段，其中所述片段保持所述全长蛋白的一种或更多种功能。在一些实施方案中，DMS3蛋白片段包含全长DMS3蛋白的至少20个连续氨基酸、至少30个连续氨基酸、至少40个连续氨基酸、至少50个连续氨基酸、至少60个连续氨基酸、至少70个连续氨基酸、至少80个连续氨基酸、至少90个连续氨基酸、至少100个连续氨基酸、至少120个连续氨基酸、至少140个连续氨基酸、至少160个连续氨基酸、至少180个连续氨基酸、至少200个连续氨基酸、至少220个连续氨基酸、至少240个连续氨基酸、或241或更多个连续氨基酸。在一些实施方案中，DMS3蛋白片段可以包括从全长DMS3蛋白的连续氨基酸序列中除去了一个或更多个氨基酸的序列。在一些实施方案中，DMS3蛋白片段可以包括在全长DMS3蛋白的连续氨基酸序列中一个或更多个氨基酸被用与存在于给定氨基酸位点的内源性氨基酸不同的氨基酸替换/置换的序列。在一些实施方案中，DMS3蛋白片段可以包括一个或更多个氨基酸被添加到全长DMS3蛋白的另外的连续氨基酸序列中的序列。
可以从单子叶植物和双子叶植物中鉴定和分离合适的DMS3蛋白。这类植物的实例包括，例如，拟南芥属种、蓖麻、大豆、玉蜀黍、蒺藜苜蓿、小立碗藓、双色蜀黍和稻。合适的DMS3蛋白的实例可包括，例如，表4中列出的那些、其同源物和其直系同源物。
表4：DMS3蛋白
在一些实施方案中，本公开内容的DMS3蛋白或其片段具有与拟南芥DMS3蛋白的氨基酸序列(即，SEQ ID NO:41)具有至少约20％、至少约25％、至少约30％、至少约40％、至少约50％、至少约55％、至少约60％、至少约65％、至少约70％、至少约75％、至少约80％、至少约85％、至少约90％、至少约91％、至少约92％、至少约93％、至少约94％、至少约95％、至少约96％、至少约97％、至少约98％、至少约99％、或至少约100％氨基酸同一性的氨基酸序列。
在一些实施方案中，DMS3蛋白的同源物，或DMS3蛋白的片段的同源物也可用于本公开内容的方法中。
DMS3样蛋白可以包括任何DMS3同源物或直系同源物，诸如例如表4中列出的那些的任意一个，的氨基酸序列或其片段。技术人员会容易地认识到另外的DMS3同源物和/或直系同源物可以存在，并且可用于本文。
MORC6蛋白
本公开内容的某些方面涉及MORC6样蛋白。在一些实施方案中，MORC6样蛋白是指重组MORC6蛋白或其片段，以及包含异源DNA结合结构域的重组MORC6蛋白或其片段。MORC6样蛋白可以用于降低植物中一种或更多种靶核酸例如基因的表达。
MORC6蛋白是本领域已知的，并记载于本文中。在一些实施方案中，本公开内容的MORC6样蛋白包括全长MORC6蛋白的功能片段，其中所述片段保持所述全长蛋白的一种或更多种功能。在一些实施方案中，MORC6蛋白片段包含全长MORC6蛋白的至少20个连续氨基酸、至少30个连续氨基酸、至少40个连续氨基酸、至少50个连续氨基酸、至少60个连续氨基酸、至少70个连续氨基酸、至少80个连续氨基酸、至少90个连续氨基酸、至少100个连续氨基酸、至少120个连续氨基酸、至少140个连续氨基酸、至少160个连续氨基酸、至少180个连续氨基酸、至少200个连续氨基酸、至少220个连续氨基酸、至少240个连续氨基酸、或241或更多个连续氨基酸。在一些实施方案中，MORC6蛋白片段可以包括从全长MORC6蛋白的连续氨基酸序列中除去了一个或更多个氨基酸的序列。在一些实施方案中，MORC6蛋白片段可以包括在全长MORC6蛋白的连续氨基酸序列中一个或更多个氨基酸被用与存在于给定氨基酸位点的内源性氨基酸不同的氨基酸替换/置换的序列。在一些实施方案中，MORC6蛋白片段可以包括一个或更多个氨基酸被添加到全长MORC6蛋白的另外的连续氨基酸序列中的序列。
可以从单子叶植物和双子叶植物中鉴定和分离合适的MORC6蛋白。这类植物的实例包括，例如，拟南芥属种、蓖麻、大豆、玉蜀黍、蒺藜苜蓿、小立碗藓、双色蜀黍和稻。合适的MORC6蛋白的实例可包括，例如，表5中列出的那些、其同源物和其直系同源物。
表5：MORC6蛋白
在一些实施方案中，本公开内容的MORC6蛋白或其片段具有与拟南芥MORC6蛋白的氨基酸序列(即，SEQ ID NO:53)具有至少约20％、至少约25％、至少约30％、至少约40％、至少约50％、至少约55％、至少约60％、至少约65％、至少约70％、至少约75％、至少约80％、至少约85％、至少约90％、至少约91％、至少约92％、至少约93％、至少约94％、至少约95％、至少约96％、至少约97％、至少约98％、至少约99％、或至少约100％氨基酸同一性的氨基酸序列。
在一些实施方案中，MORC6蛋白的同源物，或MORC6蛋白的片段的同源物也可用于本公开内容的方法中。
MORC6样蛋白可以包括任何MORC6同源物或直系同源物，诸如例如表5中列出的那些的任意一个，的氨基酸序列或其片段。技术人员会容易地认识到另外的MORC6同源物和/或直系同源物可以存在，并且可用于本文。
SUVR2蛋白
本公开内容的某些方面涉及SUVR2样蛋白。在一些实施方案中，SUVR2样蛋白是指重组SUVR2蛋白或其片段，以及包含异源DNA结合结构域的重组SUVR2蛋白或其片段。SUVR2样蛋白可以用于降低植物中一种或更多种靶核酸例如基因的表达。
SUVR2蛋白是本领域已知的，并记载于本文中。在一些实施方案中，本公开内容的SUVR2样蛋白包括全长SUVR2蛋白的功能片段，其中所述片段保持所述全长蛋白的一种或更多种功能。在一些实施方案中，SUVR2蛋白片段包含全长SUVR2蛋白的至少20个连续氨基酸、至少30个连续氨基酸、至少40个连续氨基酸、至少50个连续氨基酸、至少60个连续氨基酸、至少70个连续氨基酸、至少80个连续氨基酸、至少90个连续氨基酸、至少100个连续氨基酸、至少120个连续氨基酸、至少140个连续氨基酸、至少160个连续氨基酸、至少180个连续氨基酸、至少200个连续氨基酸、至少220个连续氨基酸、至少240个连续氨基酸、或241或更多个连续氨基酸。在一些实施方案中，SUVR2蛋白片段可以包括从全长SUVR2蛋白的连续氨基酸序列中除去了一个或更多个氨基酸的序列。在一些实施方案中，SUVR2蛋白片段可以包括在全长SUVR2蛋白的连续氨基酸序列中一个或更多个氨基酸被用与存在于给定氨基酸位点的内源性氨基酸不同的氨基酸替换/置换的序列。在一些实施方案中，SUVR2蛋白片段可以包括一个或更多个氨基酸被添加到全长SUVR2蛋白的另外的连续氨基酸序列中的序列。
可以从单子叶植物和双子叶植物中鉴定和分离合适的SUVR2蛋白。这类植物的实例包括，例如，拟南芥属种、蓖麻、大豆、玉蜀黍、蒺藜苜蓿、小立碗藓、双色蜀黍和稻。合适的SUVR2蛋白的实例可包括，例如，表6中列出的那些、其同源物和其直系同源物。
表6：SUVR2蛋白
在一些实施方案中，本公开内容的SUVR2蛋白或其片段具有与拟南芥SUVR2蛋白的氨基酸序列(即，SEQ ID NO:66)具有至少约20％、至少约25％、至少约30％、至少约40％、至少约50％、至少约55％、至少约60％、至少约65％、至少约70％、至少约75％、至少约80％、至少约85％、至少约90％、至少约91％、至少约92％、至少约93％、至少约94％、至少约95％、至少约96％、至少约97％、至少约98％、至少约99％、或至少约100％氨基酸同一性的氨基酸序列。
在一些实施方案中，SUVR2蛋白的同源物，或SUVR2蛋白的片段的同源物也可用于本公开内容的方法中。
SUVR2样蛋白可以包括任何SUVR2同源物或直系同源物，诸如例如表6中列出的那些的任意一个，的氨基酸序列或其片段。技术人员会容易地认识到另外的SUVR2同源物和/或直系同源物可以存在，并且可用于本文。
DRD1蛋白
本公开内容的某些方面涉及DRD1样蛋白。在一些实施方案中，DRD1样蛋白是指重组DRD1蛋白或其片段，以及包含异源DNA结合结构域的重组DRD1蛋白或其片段。DRD1样蛋白可以用于降低植物中一种或更多种靶核酸例如基因的表达。
DRD1蛋白是本领域已知的，并记载于本文中。在一些实施方案中，本公开内容的DRD1样蛋白包括全长DRD1蛋白的功能片段，其中所述片段保持所述全长蛋白的一种或更多种功能。在一些实施方案中，DRD1蛋白片段包含全长DRD1蛋白的至少20个连续氨基酸、至少30个连续氨基酸、至少40个连续氨基酸、至少50个连续氨基酸、至少60个连续氨基酸、至少70个连续氨基酸、至少80个连续氨基酸、至少90个连续氨基酸、至少100个连续氨基酸、至少120个连续氨基酸、至少140个连续氨基酸、至少160个连续氨基酸、至少180个连续氨基酸、至少200个连续氨基酸、至少220个连续氨基酸、至少240个连续氨基酸、或241或更多个连续氨基酸。在一些实施方案中，DRD1蛋白片段可以包括从全长DRD1蛋白的连续氨基酸序列中除去了一个或更多个氨基酸的序列。在一些实施方案中，DRD1蛋白片段可以包括在全长DRD1蛋白的连续氨基酸序列中一个或更多个氨基酸被用与存在于给定氨基酸位点的内源性氨基酸不同的氨基酸替换/置换的序列。在一些实施方案中，DRD1蛋白片段可以包括一个或更多个氨基酸被添加到全长DRD1蛋白的另外的连续氨基酸序列中的序列。
可以从单子叶植物和双子叶植物中鉴定和分离合适的DRD1蛋白。这类植物的实例包括，例如，拟南芥属种、蓖麻、大豆、玉蜀黍、小立碗藓、双色蜀黍和稻。合适的DRD1蛋白的实例可包括，例如，表7中列出的那些、其同源物和其直系同源物。
表7：DRD1蛋白
生物体 基因名 SEQ ID NO:
拟南芥 NP_179232.1 79
蓖麻 XP_002530324.1 80
大豆 XP_003540522.1 81
玉蜀黍 AFW57413.1 82
小立碗藓 XP_001752976.1 83
双色蜀黍 XP_002445019.1 84
稻 BAC84084.1 85
二穗短柄草 XP_003571619.1 86
毛果杨 XP_002313774.2 87
葡萄 XP_002273814.1 88
黄瓜 XP_004170971.1 89
琴叶拟南芥 XP_002884170.1 90
在一些实施方案中，本公开内容的DRD1蛋白或其片段具有与拟南芥DRD1蛋白的氨基酸序列(即，SEQ ID NO:79)具有至少约20％、至少约25％、至少约30％、至少约40％、至少约50％、至少约55％、至少约60％、至少约65％、至少约70％、至少约75％、至少约80％、至少约85％、至少约90％、至少约91％、至少约92％、至少约93％、至少约94％、至少约95％、至少约96％、至少约97％、至少约98％、至少约99％、或至少约100％氨基酸同一性的氨基酸序列。
在一些实施方案中，DRD1蛋白的同源物，或DRD1蛋白的片段的同源物也可用于本公开内容的方法中。
DRD1样蛋白可以包括任何DRD1同源物或直系同源物，诸如例如表7中列出的那些的任意一个，的氨基酸序列或其片段。技术人员会容易地认识到另外的DRD1同源物和/或直系同源物可以存在，并且可用于本文。
RDM1蛋白
本公开内容的某些方面涉及RDM1样蛋白。在一些实施方案中，RDM1样蛋白是指重组RDM1蛋白或其片段，以及包含异源DNA结合结构域的重组RDM1蛋白或其片段。RDM1样蛋白可以用于降低植物中一种或更多种靶核酸例如基因的表达。
RDM1蛋白是本领域已知的，并记载于本文中。在一些实施方案中，本公开内容的RDM1样蛋白包括全长RDM1蛋白的功能片段，其中所述片段保持所述全长蛋白的一种或更多种功能。在一些实施方案中，RDM1蛋白片段包含全长RDM1蛋白的至少20个连续氨基酸、至少30个连续氨基酸、至少40个连续氨基酸、至少50个连续氨基酸、至少60个连续氨基酸、至少70个连续氨基酸、至少80个连续氨基酸、至少90个连续氨基酸、至少100个连续氨基酸、至少120个连续氨基酸、至少140个连续氨基酸、至少160个连续氨基酸、至少180个连续氨基酸、至少200个连续氨基酸、至少220个连续氨基酸、至少240个连续氨基酸、或241或更多个连续氨基酸。在一些实施方案中，RDM1蛋白片段可以包括从全长RDM1蛋白的连续氨基酸序列中除去了一个或更多个氨基酸的序列。在一些实施方案中，RDM1蛋白片段可以包括在全长RDM1蛋白的连续氨基酸序列中一个或更多个氨基酸被用与存在于给定氨基酸位点的内源性氨基酸不同的氨基酸替换/置换的序列。在一些实施方案中，RDM1蛋白片段可以包括一个或更多个氨基酸被添加到全长RDM1蛋白的另外的连续氨基酸序列中的序列。
可以从单子叶植物和双子叶植物中鉴定和分离合适的RDM1蛋白。这类植物的实例包括，例如，拟南芥属种、蓖麻、大豆、玉蜀黍和稻。合适的RDM1蛋白的实例可包括，例如，表8中列出的那些、其同源物和其直系同源物。
表8：RDM1蛋白
生物体 基因名 SEQ ID NO:
拟南芥 NP_188907.2 91
蓖麻 XP_002517093.1 92
大豆 NP_001237231.1 93
玉蜀黍 NP_001170520.1 94
蒺藜苜蓿 XP_003610752.1 95
稻 BAD38576.1 96
毛果杨 XP_002311634.1 97
葡萄 XP_002279112.2 98
黄瓜 XP_004134127.1 99
琴叶拟南芥 XP_002883375.1 100
在一些实施方案中，本公开内容的RDM1蛋白或其片段具有与拟南芥RDM1蛋白的氨基酸序列(即，SEQ ID NO:91)具有至少约20％、至少约25％、至少约30％、至少约40％、至少约50％、至少约55％、至少约60％、至少约65％、至少约70％、至少约75％、至少约80％、至少约85％、至少约90％、至少约91％、至少约92％、至少约93％、至少约94％、至少约95％、至少约96％、至少约97％、至少约98％、至少约99％、或至少约100％氨基酸同一性的氨基酸序列。
在一些实施方案中，RDM1蛋白的同源物，或RDM1蛋白的片段的同源物也可用于本公开内容的方法中。
RDM1样蛋白可以包括任何RDM1同源物或直系同源物，诸如例如表8中列出的那些的任意一个，的氨基酸序列或其片段。技术人员会容易地认识到另外的RDM1同源物和/或直系同源物可以存在，并且可用于本文。
DRM3蛋白
本公开内容的某些方面涉及DRM3样蛋白。在一些实施方案中，DRM3样蛋白是指重组DRM3蛋白或其片段，以及包含异源DNA结合结构域的重组DRM3蛋白或其片段。DRM3样蛋白可以用于降低植物中一种或更多种靶核酸例如基因的表达。
DRM3蛋白是本领域已知的，并记载于本文中。在一些实施方案中，本公开内容的DRM3样蛋白包括全长DRM3蛋白的功能片段，其中所述片段保持所述全长蛋白的一种或更多种功能。在一些实施方案中，DRM3蛋白片段包含全长DRM3蛋白的至少20个连续氨基酸、至少30个连续氨基酸、至少40个连续氨基酸、至少50个连续氨基酸、至少60个连续氨基酸、至少70个连续氨基酸、至少80个连续氨基酸、至少90个连续氨基酸、至少100个连续氨基酸、至少120个连续氨基酸、至少140个连续氨基酸、至少160个连续氨基酸、至少180个连续氨基酸、至少200个连续氨基酸、至少220个连续氨基酸、至少240个连续氨基酸、或241或更多个连续氨基酸。在一些实施方案中，DRM3蛋白片段可以包括从全长DRM3蛋白的连续氨基酸序列中除去了一个或更多个氨基酸的序列。在一些实施方案中，DRM3蛋白片段可以包括在全长DRM3蛋白的连续氨基酸序列中一个或更多个氨基酸被用与存在于给定氨基酸位点的内源性氨基酸不同的氨基酸替换/置换的序列。在一些实施方案中，DRM3蛋白片段可以包括一个或更多个氨基酸被添加到全长DRM3蛋白的另外的连续氨基酸序列中的序列。
可以从单子叶植物和双子叶植物中鉴定和分离合适的DRM3蛋白。这类植物的实例包括，例如，拟南芥属种、蓖麻、大豆、玉蜀黍、小立碗藓、双色蜀黍和稻。合适的DRM3蛋白的实例可包括，例如，表9中列出的那些、其同源物和其直系同源物。
表9：DRM3蛋白
生物 基因名 SEQ ID NO:
拟南芥 NP_566573.1 101
蓖麻 XP_002519294.1 102
大豆 XP_006583974.1 103
玉蜀黍 NP_001105094.1 104
蒺藜苜蓿 XP_003609841.1 105
双色蜀黍 XP_002468285.1 106
稻 AAT85176.1 107
二穗短柄草 XP_003569077.1 108
毛果杨 XP_002316067.2 109
葡萄 XP_002264226.1 110
黄瓜 XP_004138523.1 111
琴叶拟南芥 XP_002885200.1 112
在一些实施方案中，本公开内容的DRM3蛋白或其片段具有与拟南芥DRM3蛋白的氨基酸序列(即，SEQ ID NO:101)具有至少约20％、至少约25％、至少约30％、至少约40％、至少约50％、至少约55％、至少约60％、至少约65％、至少约70％、至少约75％、至少约80％、至少约85％、至少约90％、至少约91％、至少约92％、至少约93％、至少约94％、至少约95％、至少约96％、至少约97％、至少约98％、至少约99％、或至少约100％氨基酸同一性的氨基酸序列。
在一些实施方案中，DRM3蛋白的同源物，或DRM3蛋白的片段的同源物也可用于本公开内容的方法中。
DRM3样蛋白可以包括任何DRM3同源物或直系同源物，诸如例如表9中列出的那些的任意一个，的氨基酸序列或其片段。技术人员会容易地认识到另外的DRM3同源物和/或直系同源物可以存在，并且可用于本文。
DRM2蛋白
本公开内容的某些方面涉及DRM2样蛋白。在一些实施方案中，DRM2样蛋白是指重组DRM2蛋白或其片段，以及包含异源DNA结合结构域的重组DRM2蛋白或其片段。DRM2样蛋白可以用于降低植物中一种或更多种靶核酸例如基因的表达。
DRM2蛋白是本领域已知的，并记载于本文中。在一些实施方案中，本公开内容的DRM2样蛋白包括全长DRM2蛋白的功能片段，其中所述片段保持所述全长蛋白的一种或更多种功能。在一些实施方案中，DRM2蛋白片段包含全长DRM2蛋白的至少20个连续氨基酸、至少30个连续氨基酸、至少40个连续氨基酸、至少50个连续氨基酸、至少60个连续氨基酸、至少70个连续氨基酸、至少80个连续氨基酸、至少90个连续氨基酸、至少100个连续氨基酸、至少120个连续氨基酸、至少140个连续氨基酸、至少160个连续氨基酸、至少180个连续氨基酸、至少200个连续氨基酸、至少220个连续氨基酸、至少240个连续氨基酸、或241或更多个连续氨基酸。在一些实施方案中，DRM2蛋白片段可以包括从全长DRM2蛋白的连续氨基酸序列中除去了一个或更多个氨基酸的序列。在一些实施方案中，DRM2蛋白片段可以包括在全长DRM2蛋白的连续氨基酸序列中一个或更多个氨基酸被用与存在于给定氨基酸位点的内源性氨基酸不同的氨基酸替换/置换的序列。在一些实施方案中，DRM2蛋白片段可以包括一个或更多个氨基酸被添加到全长DRM2蛋白的另外的连续氨基酸序列中的序列。
可以从单子叶植物和双子叶植物中鉴定和分离合适的DRM2蛋白。这类植物的实例包括，例如，拟南芥属种、蓖麻、大豆、玉蜀黍、小立碗藓、双色蜀黍和稻。合适的DRM2蛋白的实例可包括，例如，表10中列出的那些、其同源物和其直系同源物。
表10：DRM2蛋白
生物 基因名 SEQ ID NO:
拟南芥 NP_196966.2 113
蓖麻 XP_002521449.1 114
大豆 XP_003524549.1 115
玉蜀黍 NP_001104977.1 116
蒺藜苜蓿 XP_003618189.1 117
双色蜀黍 XP_002468660.1 118
稻 ABF93591.1 119
二穗短柄草 XP_003575456.1 120
毛果杨 XP_002300046.2 121
葡萄 XP_002273972.2 122
黄瓜 XP_004141100.1 123
琴叶拟南芥 XP_002873681.1 124
在一些实施方案中，本公开内容的DRM2蛋白或其片段具有与拟南芥DRM2蛋白的氨基酸序列(即，SEQ ID NO:113)具有至少约20％、至少约25％、至少约30％、至少约40％、至少约50％、至少约55％、至少约60％、至少约65％、至少约70％、至少约75％、至少约80％、至少约85％、至少约90％、至少约91％、至少约92％、至少约93％、至少约94％、至少约95％、至少约96％、至少约97％、至少约98％、至少约99％、或至少约100％氨基酸同一性的氨基酸序列。
在一些实施方案中，DRM2蛋白的同源物，或DRM2蛋白的片段的同源物也可用于本公开内容的方法中。
DRM2样蛋白可以包括任何DRM2同源物或直系同源物，诸如例如表10中列出的那些的任意一个，的氨基酸序列或其片段。技术人员会容易地认识到另外的DRM2同源物和/或直系同源物可以存在，并且可用于本文。
FRG蛋白
本公开内容的某些方面涉及FRG样蛋白。在一些实施方案中，FRG样蛋白是指重组FRG蛋白或其片段，以及包含异源DNA结合结构域的重组FRG蛋白或其片段。FRG样蛋白可以用于降低植物中一种或更多种靶核酸例如基因的表达。
FRG蛋白是本领域已知的，并记载于本文中。在一些实施方案中，本公开内容的FRG样蛋白包括全长FRG蛋白的功能片段，其中所述片段保持所述全长蛋白的一种或更多种功能。在一些实施方案中，FRG蛋白片段包含全长FRG蛋白的至少20个连续氨基酸、至少30个连续氨基酸、至少40个连续氨基酸、至少50个连续氨基酸、至少60个连续氨基酸、至少70个连续氨基酸、至少80个连续氨基酸、至少90个连续氨基酸、至少100个连续氨基酸、至少120个连续氨基酸、至少140个连续氨基酸、至少160个连续氨基酸、至少180个连续氨基酸、至少200个连续氨基酸、至少220个连续氨基酸、至少240个连续氨基酸、或241或更多个连续氨基酸。在一些实施方案中，FRG蛋白片段可以包括从全长FRG蛋白的连续氨基酸序列中除去了一个或更多个氨基酸的序列。在一些实施方案中，FRG蛋白片段可以包括在全长FRG蛋白的连续氨基酸序列中一个或更多个氨基酸被用与存在于给定氨基酸位点的内源性氨基酸不同的氨基酸替换/置换的序列。在一些实施方案中，FRG蛋白片段可以包括一个或更多个氨基酸被添加到全长FRG蛋白的另外的连续氨基酸序列中的序列。
可以从单子叶植物和双子叶植物中鉴定和分离合适的FRG蛋白。这类植物的实例包括，例如，拟南芥属种、蓖麻、大豆、玉蜀黍、小立碗藓、双色蜀黍和稻。合适的FRG蛋白的实例可包括，例如，表11中列出的那些、其同源物和其直系同源物。
表11：FRG蛋白
在一些实施方案中，本公开内容的FRG蛋白或其片段具有与拟南芥FRG蛋白的氨基酸序列(即，SEQ ID NO:125)具有至少约20％、至少约25％、至少约30％、至少约40％、至少约50％、至少约55％、至少约60％、至少约65％、至少约70％、至少约75％、至少约80％、至少约85％、至少约90％、至少约91％、至少约92％、至少约93％、至少约94％、至少约95％、至少约96％、至少约97％、至少约98％、至少约99％、或至少约100％氨基酸同一性的氨基酸序列。
在一些实施方案中，FRG蛋白的同源物，或FRG蛋白的片段的同源物也可用于本公开内容的方法中。
FRG样蛋白可以包括任何FRG同源物或直系同源物，诸如例如表11中列出的那些的任意一个的氨基酸序列或其片段。技术人员会容易地认识到另外的FRG同源物和/或直系同源物可以存在，并且可用于本文。
DNA结合结构域
本公开内容的SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白具有DNA结合活性。该DNA结合活性通过异源DNA结合结构域实现。在一些实施方案中，本公开内容的重组蛋白包含DNA结合结构域。本公开内容的重组蛋白可以包含一个DNA结合结构域，或者它们可以包含多于一个DNA结合结构域。
在一些实施方案中，DNA结合结构域是锌指结构域。如本文所公开，“锌指结构域”是指包含锌指的DNA结合蛋白质结构域，所述锌指是能够配合一个或更多个锌离子以帮助稳定它们的蛋白质折叠的小的蛋白质结构基序。锌指一般可被分类为若干不同的结构家族，并且一般作为结合DNA、RNA、蛋白质或小分子的相互作用模块起作用。本公开内容的合适的锌指结构域可包含2个、3个、4个、5个、6个、7个、8个或9个锌指。合适的锌指结构域的实例可包括例如Cys2His2(C2H2)锌指结构域、C-x8-C-x5-C-x3-H(CCCH)锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
在一些实施方案中，所述DNA结合结构域结合特定的核酸序列。例如，所述DNA结合结构域可结合长度为至少5个核苷酸、至少6个核苷酸、至少7个核苷酸、至少8个核苷酸、至少9个核苷酸、至少10个核苷酸、至少11个核苷酸、至少12个核苷酸、至少13个核苷酸、至少14个核苷酸、至少15个核苷酸、至少20个核苷酸、至少25个核苷酸、至少30个核苷酸、至少35个核苷酸、至少40个核苷酸、至少45个核苷酸、至少50个核苷酸或更高数目的核苷酸的序列。在一些实施方案中，DNA结合结构域结合长度为8个核苷酸的序列。
在一些实施方案中，本公开内容的重组蛋白还包含两个N末端CCCH锌指结构域。
在一些实施方案中，锌指结构域是工程化的锌指阵列，例如C2H2锌指阵列。工程化的C2H2锌指的阵列能够用于产生能够靶向所需基因组DNA序列的DNA结合蛋白。工程化锌指阵列的方法是本领域公知的，并且包括，例如组合具有已知特异性的较小锌指。
在一些实施方案中，重组蛋白可以包含除了锌指结构域之外的DNA结合结构域。这种DNA结合结构域的实例可包括，例如，TAL(转录激活因子样)效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP(TATA-盒结合蛋白)结构域、桶状二聚体结构域、RHB结构域(真实同源结构域、BAH(布罗莫临近同源性(bromo-adjacent homology))结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域(植物同源异型结构域)、WD40结构域和MBD结构域(甲基-CpG-结合结构域)。
在一些实施方案中，DNA结合结构域是TAL效应子靶向结构域。如本文所用的，TAL效应子是指分泌的细菌蛋白，例如由黄单胞菌属(Xanthomonas)或罗尔斯通菌属(Ralstonia)细菌感染多种植物物种时分泌的那些。通常，TAL效应子能够结合宿主植物中的启动子序列，并且激活有助于细菌感染的植物基因的表达。TAL效应子通过中心重复靶向结构域识别植物DNA序列，所述中心重复靶向结构域包含可变数量的约34个氨基酸重复。此外，TAL效应子靶向结构域可以被工程化以靶向特定的DNA序列。修饰TAL效应子靶向结构域的方法是本领域公知的，并且记载于Bogdanove和Voytas,Science.2011 Sep 30；333(6051):1843-6)。
本公开内容的靶核酸
本公开内容的其他方面涉及利用重组蛋白通过与感兴趣的基因相关的一种或更多种靶核酸结合而降低植物中一种或更多种感兴趣的基因的表达。重组蛋白可以是SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白。在一些实施方案中，SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白通过与靶核酸结合而降低感兴趣的基因的表达。在一些实施方案中，SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白通过与靶核酸结合而使感兴趣的基因的表达沉默。
在一些实施方案中，本公开内容的靶核酸是位于靶基因内任何位置处的提供用于降低靶基因表达的适合位置的核酸。靶核酸可以位于靶基因的编码区或其上游或下游。此外，靶核酸可以内源性地存在于靶基因中，或者可以例如使用诸如同源重组的技术被插入到基因中(例如异源性的)。例如，本公开内容的靶基因可以被可操作地连接至控制区，例如启动子，所述控制区包含被本公开内容的SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白识别并结合的序列。
靶核酸可以是能够被本公开内容的SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白结合的任何给定的感兴趣的核酸。在一些实施方案中，靶核酸对于植物是内源性的，在所述植物中一种或更多种基因的表达被本公开内容的SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白降低。在一些实施方案中，靶核酸是已经被插入到植物中的感兴趣的转基因。将转基因引入植物中的方法是本领域熟知的。可以将转基因插入植物中以提供用于所需蛋白的生产系统，或者可以将转基因添加到遗传互补物中以调节植物的代谢。
其表达可以被本公开内容的SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白降低的合适的内源性植物基因的实例可以包括，例如，阻止一种或更多种所需特性的增强的基因和阻止增加的作物产量的基因。例如，本公开内容的SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白可以用于降低植物中基因GAI的表达，这会产生对赤霉素较不敏感的植物。在与研究相关的实施方案中，本公开内容的SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白可以用于使感兴趣的内源性基因的表达沉默，以产生在其中研究所述感兴趣的基因的功能的突变植物。
存在于植物中的其表达可以被本公开内容的SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白降低的合适的转基因的实例可以包括，例如，在某些遗传背景中没有用处的转基因、在某些遗传背景中有害的转基因以及在某些不期望的组织中表达的转基因。例如，在某些不期望的组织中表达的转基因的情况下，本公开内容的SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白能够用于通过将组织特异性启动子可操作地连接至本公开内容的重组多肽，而在特定时间在特定组织中使此类转基因的表达沉默。在与研究相关的实施方案中，本公开内容的SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白可以用于通过控制转基因的诱导/沉默，而动态研究感兴趣的转基因。
本公开内容的植物
本公开内容的某些方面涉及包含一种或更多种SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白的植物。在某些实施方案中，SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白与植物中的一种或更多种靶核酸结合，并降低一种或更多种靶核酸的表达。
如本文所用的，“植物”是指植物界的多种光合作用的、真核多细胞生物的任一种，特征为产生胚、包含叶绿体、具有纤维素细胞壁并缺乏运动性。如本文所用的，“植物”包括在任何发育阶段的任何植物或植物部分，包括种子、悬浮培养物、植物细胞、胚、分生区、愈伤组织、叶、根、嫩枝、配子体、孢子体、花粉、小孢子及其后代。还包括插条以及细胞或组织培养物。如与本公开内容联合使用的，植物组织包括，不限于，全植物、植物细胞、植物器官(例如叶、茎、根)、分生组织、植物种子、原生质体、愈伤组织、细胞培养物以及组成结构和/或功能单位的任何植物细胞群体。
任何植物细胞可以用于本公开内容中，只要其在用核酸序列转化之后保持活力。优选地，植物细胞不会受到必需核酸序列的转导、后续的蛋白表达或得到中间产物的负面影响。
如本文公开的，宽范围的植物类型可以被修饰以并入SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白。可以被修饰的合适的植物包括单子叶(monocotyledonous)(单子叶(monocot))植物和双子叶(dicotyledonous)(双子叶(dicot))植物。
合适的植物的实例可以包括，例如，禾本科(Gramineae)的物种，包括两色蜀黍(Sorghum bicolor)和玉蜀黍(Zea mays)；以下属的物种：南瓜属(Cucurbita)、蔷薇属(Rosa)、葡萄属(Vitis)、胡桃属(Juglans)、草莓属(Fragaria)、百脉根属(Lotus)、苜蓿属(Medicago)、驴食草属(Onobrychis)、三叶草属(Trifolium)、葫芦巴属(Trigonella)、豇豆属(Vigna)、柑橘属(Citrus)、亚麻属(Linum)、老鹳草属(Geranium)、木薯属(Manihot)、胡萝卜属(Daucus)、拟南芥属(Arabidopsis)、芸苔属(Brassica)、萝卜属(Raphanus)、白芥属(Sinapis)、颠茄属(Atropa)、辣椒属(Capsicum)、曼陀罗属(Datura)、莨菪属(Hyoscyamus)、番茄属(Lycopersicon)、烟草属(Nicotiana)、茄属(Solanum)、碧冬茄属(Petunia)、毛地黄属(Digitalis)、Majorana、菊苣属(Ciahorium)、向日葵属(Helianthus)、莴苣属(Lactuca)、雀麦属(Bromus)、天门冬属(Asparagus)、金鱼草属(Antirrhinum)、Heterocallis、Nemesis、天竺葵属(Pelargonium)、黍属(Panieum)、狼尾草属(Pennisetum)、毛茛属(Ranunculus)、千里光属(Senecio)、喇叭舌属(Salpiglossis)、甜瓜属(Cucumis)、蓝英花属(Browaalia)、大豆属(Glycine)、豌豆属(Pisum)、菜豆属(Phaseolus)、黑麦草属(Lolium)、稻属(Oryza)、燕麦属(Avena)、大麦属(Hordeum)、黑麦属(Secale)和小麦属(Triticum)。
在一些实施方案中，植物细胞可以包括，例如，来自玉米(玉蜀黍)、油菜(甘蓝型油菜(Brassica napus)、芜菁(Brassica rapa spp.)、用作种子油来源的芸苔属种、紫花苜蓿(alfalfa)(紫花苜蓿(Medicago sativa))、稻(rice)(稻(Oryza sativa))、黑麦(rye)(黑麦(Secale cereale))、高粱(sorghum)(两色蜀黍、高粱(Sorghum vulgare))、稷(millet)(例如御谷(pearl millet)(御谷(Pennisetum glaucum))、黍(proso millet)(黍(Panicummiliaceum))、粟(foxtail millet)(粟(Setaria italica))、龙爪稷(finger millet)(龙爪稷(Eleusine coracana)))、向日葵(sunflower)(向日葵(Helianthus annuus))、红花(safflower)(红花(Carthamus tinctorius))、小麦(wheat)(小麦(Triticum aestivum))、浮萍(duckweed)(浮萍属(Lemna))、大豆(soybean)(大豆(Glycine max))、烟草(tobacco)(烟草(Nicotiana tabacum))、马铃薯(potato)(马铃薯(Solanum tuberosum))、花生(pwanuts)(花生(Arachis hypogaea))、棉花(cotton)(海岛棉(Gossypium barbadense)、陆地棉(Gossypium hirsutum))、番薯(sweet potato)(番薯(Ipomoea batatus))、木薯(cassava)(木薯(Manihot esculenta))、咖啡(咖啡属种(Coffea spp.))、椰子(cocnut)(Cocos nucijra)、凤梨(pineapple)(凤梨(Ananas comosus))、柑橘树(柑橘属种(Citrusspp.))、可可(cocoa)(可可(Theobroma cacao))、茶(野茶树(Camellia sinensis))、香蕉(芭蕉属种(Musa spp.))、鳄梨(avocado)(鳄梨(Persea americana))、无花果(fig)(无花果(Ficus casica))、番石榴(guava)(番石榴(Psidium guajava))、芒果(mango)(芒果(Mangifera indica))、橄榄(olive)(橄榄(Olea europaea))、番木瓜(papaya)(番木瓜(Carica papaya))、腰果(cashew)(腰果(Anacardium occidentale))、澳洲坚果(澳洲坚果属种(Macadamia spp.))、巴旦杏(almond)(巴旦杏(Prunus amygdalus))、甜菜(sugarbeets)(甜菜(Beta vulgaris))、甘蔗(甘蔗属种(Saccharum spp.))、燕麦、大麦、蔬菜、观赏植物和松柏类植物的那些。
合适的蔬菜植物的实例可以包括，例如，番茄(tomatoes)(番茄(Lycopersiconesculentum))、莴苣(lettuce)(例如莴苣(Lactuca sativa))、青豆(菜豆(Phaseolusvulgaris))、利马豆(lima beans)(利马豆(Phaseolus limensis))、豌豆(山黧豆属种(Lathyrus spp.))以及甜瓜属的成员例如黄瓜(cucumber)(黄瓜(C.sativus))、甜瓜(罗马甜瓜(C.cantalupensis))和香瓜(musk melon)(香瓜(C.melo))。
合适的观赏植物的实例可以包括，例如，杜鹃花(杜鹃花属种(Rhododendronspp.))、绣球花(hydrangea)(绣球花(Macrophylla hydrangea))、木槿(hibiscus)(木槿(Hibiscus rosasanensis))、蔷薇(蔷薇属种(Rosa spp.))、郁金香(郁金香属种(Tulipaspp.))、水仙(水仙属种(Narcissus spp.))、矮牵牛(petunias)(矮牵牛(Petuniahybrida))、康乃馨(carnation)(康乃馨(Dianthus caryophyllus))、一品红(poinsettia)(一品红(Euphorbiapulcherrima))和菊花。
合适的松柏类植物的实例可以包括，例如，火炬松(loblolly pine)(火炬松(Pinus taeda))、湿地松(slash pine)(湿地松(Pinus elliotii))、西黄松(ponderosapine)(西黄松(Pinus ponderosa))、美国黑松(lodgepole pine)(美国黑松(Pinuscontorta))、辐射松(Monterey pine)(辐射松(Pinus radiata))、花旗松(Douglas-fir)(花旗松(Pseudotsuga menziesii))、西部铁杉(Isuga canadensis)、西加云杉(Siktaspruce)(白云杉(Picea glauca))、红杉(北美红杉(Sequoia sempervirens)、银杉(太平洋银冷杉(Abies amabilis))、香脂冷杉(balsam fir)(香脂冷杉(Abies balsamea))、西部红松(Western red cedar)(西部红松(Thuja plicata))和阿拉斯加黄柏(Alaska yellow-cedar)(拿加逊扁柏(Chamaecyparis nootkatensis))。
合适的豆科植物的实例可以包括，例如，瓜尔豆、槐豆、葫芦巴、大豆、四季豆(garden beans)、豇豆、绿豆、利马豆、蚕豆、扁豆、鹰嘴豆、花生(花生属种(Arachis sp.))、多变小冠花(crown vetch)(蚕豆属种(Vicia sp.))、毛叶苕子、小豆、羽扇豆(羽扇豆属种(Lupinus sp.))、三叶草、菜豆(菜豆属种(Phaseolus sp.))、菜豆(field bean)(豌豆属种(Pisum sp.))、三叶草(clover)(草木樨属种(Melilotus sp.))百脉根属(Lotus)、车轴草、兵豆属和紫穗槐。
合适的牧草和草坪草的实例可以包括，例如，紫花苜蓿(苜蓿属种(Medicagospp.))、野茅、高羊茅、黑麦草、匍匐翦股颖和小糠草。
合适的作物植物和模式植物的实例可以包括，例如，拟南芥、玉米、稻、紫花苜蓿、向日葵、油菜、大豆、棉花、花生、高粱、小麦、烟草和浮萍属。
本公开内容的植物可以被遗传修饰是由于重组核酸已经被引入所述植物中，并且如此遗传修饰的植物不会在天然状态下出现。本公开内容的合适的植物是能够表达编码一种或更多种重组蛋白的一种或更多种核酸构建体的植物。核酸编码的重组蛋白可以是SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白。
如本文所用的，术语“转基因植物”和“遗传修饰的植物”可以互换使用，并且是指在其基因组中包含重组核酸的植物。一般来说，重组核酸被稳定地整合在基因组中，使得多核苷酸能够被传递到连续世代。但是，在某些实施方案中，重组核酸在植物中瞬时表达。重组核酸可以单独或作为重组表达盒的一部分被整合到基因组中。在本文中使用“转基因(transgenic)”以包括其基因型已经通过外源核酸的存在而被改变的任何细胞、细胞系、愈伤组织、组织、植物部分或植物，所述外源核酸的存在包括最初如此改变的那些转基因产物，以及通过有性杂交或无性繁殖从最初转基因产物产生的那些。
如本文使用的“重组核酸”或“异源核酸”或“重组多核苷酸”是指核酸的聚合物，其中以下的至少一种是真实的：(a)核酸序列对于给定的宿主细胞是外来的(即，不是天然存在于给定的宿主细胞中的)；(b)序列可以天然存在于给定的宿主细胞，但是是以非天然(例如比预期更高)的量存在；或(c)核酸序列包含两个或更多个子序列，所述子序列在天然条件下不存在于相同的相互关系中。例如，关于情况(c)，重组核酸序列将具有来自不相关基因的两个或更多个序列，所述两个或更多个序列被排列以制成新的功能性核酸。具体地，本公开内容描述了将表达载体引入植物细胞，其中表达载体包含编码通常不存在于植物细胞中的蛋白的核酸序列，或包含编码通常存在于植物细胞但是在不同调节序列的控制下的蛋白的核酸。然后，参照植物细胞的基因组，编码蛋白的核酸序列是重组的。被称为重组体的蛋白通常暗示其是由植物细胞中的重组核酸序列编码的。
本公开内容的“重组”多肽、蛋白或酶是由“重组核酸”或“异源性核酸”或“重组多核苷酸”编码的多肽、蛋白或酶。
在一些实施方案中，植物细胞中编码重组蛋白的基因相对于所述植物细胞可以是异源性的。在某些实施方案中，植物细胞不会天然地产生重组蛋白，并包含能够表达用于产生那些分子所必需的一种或更多种基因的异源核酸构建体。
植物中重组蛋白的表达
可以经由本领域已知的任何合适的方法将本公开内容的SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白引入植物细胞中。例如，可以将SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白外源性地添加至植物细胞，并且将植物细胞维持在使得SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白与一种或更多种靶核酸结合并降低植物细胞中靶核酸的表达的条件下。可选地，本公开内容的编码SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白的重组核酸能够在植物细胞中表达，并且将植物细胞维持在使得所表达的SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白与一种或更多种靶核酸结合并降低植物细胞中靶基因的表达的条件下。此外，在一些实施方案中，可以经由病毒感染植物或通过将SHH1样蛋白编码RNA、SUVH2样蛋白编码RNA、SUVH9样蛋白编码RNA、DMS3样蛋白编码RNA、MORC6样蛋白编码RNA、SUVR2样蛋白编码RNA、DRD1样蛋白编码RNA、RDM1样蛋白编码RNA、DRM3样蛋白编码RNA、DRM2样蛋白编码RNA和/或FRG样蛋白编码RNA引入植物中，而在植物细胞中瞬时表达本公开内容的SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白，以暂时性降低感兴趣的基因的表达或使感兴趣的基因的表达沉默。经由病毒感染或经由将RNA引入植物中而引入重组蛋白的方法是本领域公知的。例如，烟草脆裂病毒(TRV)已被成功地用于将锌指核酸酶引入植物中以引起基因组修饰(“Nontransgenic Genome Modification in Plant Cells”,Plant Physiology 154:1079-1087(2010))。
本公开内容的编码SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白的重组核酸可使用任何合适的植物表达载体在植物中表达。可用于在高等植物中表达重组核酸的典型载体是本领域公知的，并且包括，不限于，衍生自根癌农杆菌(Agrobacteriumtumefaciens)的肿瘤诱导(Ti)质粒的载体(例如，参见Rogers等人,Meth.in Enzymol.(1987),153:253-277)。这些载体是植物整合载体的原因在于，在转化中，载体将载体DNA的部分整合到宿主植物的基因组中。可用于本文的示例性根癌农杆菌载体是质粒pKYLX6和pKYLX7(例如，参见Schardl等人,Gene(1987)61:1-11，和Berger等人,Proc.Natl.Acad.Sci.USA(1989)86:8402-8406)；以及从Clontech Laboratories,Inc.(Palo Alto,CA)可得的质粒pBI 101.2。
除了调节结构域之外，可将本公开内容的SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白作为与以下偶联的融合蛋白表达：例如以便于纯化、监测表达或监测细胞和亚细胞定位的麦芽糖结合蛋白(“MBP”)、谷胱甘肽S转移酶(GST)、六聚组氨酸、c-myc或FLAG表位。
此外，可通过使用密码子偏好性修饰本公开内容的编码SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白的重组核酸，以提高植物中重组蛋白的表达。当合成性地制备或改变重组核酸时，可利用核酸待在其中表达的预期的植物宿主的已知密码子偏好性。例如，可修饰本公开内容的重组核酸以产生单子叶植物和双子叶植物的特定的密码子偏好性和GC含量偏好性，因为已经表明这些偏好性有差异(Murray等人,Nucl.Acids Res.(1989)17:477-498)。
在一些实施方案中，本公开内容的SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白可用于通过抑制靶基因表达而在植物中产生功能性的“基因敲除”突变。抑制可以是对结构基因的抑制，所述结构基因例如编码具有例如酶活性的蛋白的基因，或是对调节基因的抑制，所述调节基因例如编码转而调节结构基因表达的蛋白的基因。
本公开内容还提供了表达载体，所述表达载体包含本公开内容的SHH1样蛋白编码核酸、SUVH2样蛋白编码核酸、SUVH9样蛋白编码核酸、DMS3样蛋白编码核酸、MORC6样蛋白编码核酸、SUVR2样蛋白编码核酸、DRD1样蛋白编码核酸、RDM1样蛋白编码核酸、DRM3样蛋白编码核酸、DRM2样蛋白编码核酸和/或FRG样蛋白编码核酸。可将编码本公开内容的期望的重组核酸的核酸序列用于构建可被引入期望的宿主细胞中的重组表达载体。重组表达载体将通常包含可操作地连接至转录起始调节序列的本公开内容的编码重组蛋白的核酸，所述转录起始调节序列将指导核酸在期望的宿主细胞例如转化的植物的组织中的转录。
例如，植物表达载体可包括(1)在5'和3'调节序列的转录调控下的克隆的植物基因和(2)显性可选择标记。如果需要的话，这类植物表达载体还可包含启动子调控区(例如，赋予诱导型的或组成型的，环境或发育调控的，或细胞或组织特异性的/选择性的表达的启动子调控区)、转录起始开始位点、核糖体结合位点、RNA加工信号、转录终止位点和/或聚腺苷酸化信号。
可采用植物启动子或其功能片段调控再生植物中本公开内容的重组核酸的表达。表达载体中使用的启动子的选择将决定改造的植物中重组核酸的时空表达模式，例如，SHH1样蛋白编码核酸、SUVH2样蛋白编码核酸、SUVH9样蛋白编码核酸、DMS3样蛋白编码核酸、MORC6样蛋白编码核酸、SUVR2样蛋白编码核酸、DRD1样蛋白编码核酸、RDM1样蛋白编码核酸、DRM3样蛋白编码核酸、DRM2样蛋白编码核酸和/或FRG样蛋白编码核酸仅在期望的组织中表达，或仅在植物发育或生长的特定时间表达。某些启动子将在所有植物组织中表达重组核酸，并且在大多数的环境条件和发育状态或细胞分化状态下是有活性的(即，组成型启动子)。其他启动子将在特定细胞类型(例如叶表皮细胞、叶肉细胞、根皮层细胞)中或在特定组织或器官(例如根、叶或花)中表达重组核酸，并且该选择将反映基因产物积累的期望位置。可选地，所选择的启动子可在多种诱导条件下驱动重组核酸的表达。
合适的组成型启动子的实例可以包括，例如，Rsyn7的核心启动子、核心CaMV 35S启动子(Odell等人,Nature(1985)313:810-812)、CaMV 19S(Lawton等人,1987)、水稻肌动蛋白(Wang等人,1992；美国专利号5,641,876；和McElroy等人,Plant Cell(1985)2:163-171)；泛素(Christensen等人,Plant Mol.Biol.(1989)12:619-632；和Christensen等人,Plant Mol.Biol.(1992)18:675-689)、pEMU(Last等人,Theor.Appl.Genet.(1991)81:581-588)、MAS(Velten等人,EMBO J.(1984)3:2723-2730)、nos(Ebert等人,1987)、Adh(Walker等人,1987)、衍生自根癌农杆菌的T-DNA的P-或2'-启动子、Smas启动子、肉桂醇脱氢酶启动子(美国专利号5,683,439)、Nos启动子、pEmu启动子、rubisco启动子、GRP 1-8启动子以及技术人员已知的来自多个植物基因的其他转录起始区、和例如美国专利号5,608,149、5,608,144、5,604,121、5,569,597、5,466,785、5,399,680、5,268,463和5,608,142中描述的组成型启动子。
合适的组织特异性启动子的实例可包括，例如，凝集素启动子(Vodkin等人,1983；Lindstrom等人,1990)、玉米醇脱氢酶1启动子(Vogel等人,1989；Dennis等人,1984)、玉米捕光复合物启动子(Simpson,1986；Bansal等人,1992)、玉米热休克蛋白启动子(Odell等人,Nature(1985)313:810-812；Rochester等人,1986)、豌豆小亚基RuBP羧化酶启动子(Poulsen等人,1986；Cashmore等人,1983)、Ti质粒甘露碱型(mannopine)合酶启动子(Langridge等人,1989)、Ti质粒胭脂碱合酶启动子(Langridge等人,1989)、矮牵牛查尔酮异构酶启动子(Van Tunen等人,1988)、豆富含甘氨酸蛋白1启动子(Keller等人,1989)、截短的CaMV 35s启动子(Odell等人,Nature(1985)313:810-812)、马铃薯patatin启动子(Wenzler等人,1989)、根细胞启动子(Conkling等人,1990)、玉米醇溶蛋白启动子(Reina等人,1990；Kriz等人,1987；Wandelt和Feix,1989；Langridge和Feix,1983；Reina等人,1990)、球蛋白-1启动子(Belanger和Kriz等人,1991)、α-微管蛋白启动子、cab启动子(Sullivan等人,1989)、PEPC酶启动子(Hudspeth&Grula,1989)、R基因复合物相关的启动子(Chandler等人,1989)、和查尔酮合酶启动子(Franken等人,1991)。
可选地，植物启动子能够指导本公开内容的重组核酸在特定组织中表达或可另外地在更加精确的环境或发育控制下表达。这类启动子在本文中被称为“诱导型”启动子。可以通过诱导型启动子影响转录的环境条件包括，不限于，病原体攻击、厌氧条件或光的存在。诱导型启动子的实例包括，不限于，可由低氧或冷应激诱导的AdhI启动子、可由热应激诱导的Hsp70启动子和可由光诱导的PPDK启动子。在发育控制下的启动子的实例包括，不限于，仅或优先在特定组织中起始转录的启动子，所述特定组织例如叶、根、果实、种子或花。一个示例性的启动子是花药特异性启动子5126(美国专利号5,689,049和5,689,051)。启动子的操作还可取决于其在基因组中的位置而不同。因此，诱导型启动子可以在某些位置变为完全或部分组成型的。
此外，组成型或诱导型启动子与非组织特异性或组织特异性启动子的任意组合可用于调控SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白的表达。
异源性和内源性启动子二者均可用于指导本公开内容的重组核酸的表达。因此，在某些实施方案中，本公开内容的SHH1样蛋白编码核酸、SUVH2样蛋白编码核酸、SUVH9样蛋白编码核酸、DMS3样蛋白编码核酸、MORC6样蛋白编码核酸、SUVR2样蛋白编码核酸、DRD1样蛋白编码核酸、RDM1样蛋白编码核酸、DRM3样蛋白编码核酸、DRM2样蛋白编码核酸和/或FRG样蛋白编码核酸的表达在其各自内源性启动子的调控下。在其他实施方案中，本公开内容的SHH1样蛋白编码核酸、SUVH2样蛋白编码核酸、SUVH9样蛋白编码核酸、DMS3样蛋白编码核酸、MORC6样蛋白编码核酸、SUVR2样蛋白编码核酸、DRD1样蛋白编码核酸、RDM1样蛋白编码核酸、DRM3样蛋白编码核酸、DRM2样蛋白编码核酸和/或FRG样蛋白编码核酸的表达在异源性启动子的调控下。另外，可使用敲入方法修饰本公开内容的内源性SHH1基因、SUVH2基因、SUVH9基因、DMS3基因、MORC6基因、SUVR2基因、DRD1基因、RDM1基因、DRM3基因、DRM2基因和/或FRG基因，使得修饰的基因将在其各自的内源性元件的调控下。可选地，可以将完整的SHH1、SUVH2、SUVH9、DMS3、MORC6、SUVR2、DRD1、RDM1、DRM3、DRM2和/或FRG基因组序列的修饰形式引入植物中，使得修饰的/重组的基因将在其内源性元件的调控下，并且野生型基因保持完整。还可将这些技术的任一种或全部组合以指导本公开内容的重组核酸的表达。
本公开内容的重组核酸，例如SHH1样蛋白编码核酸、SUVH2样蛋白编码核酸、SUVH9样蛋白编码核酸、DMS3样蛋白编码核酸、MORC6样蛋白编码核酸、SUVR2样蛋白编码核酸、DRD1样蛋白编码核酸、RDM1样蛋白编码核酸、DRM3样蛋白编码核酸、DRM2样蛋白编码核酸和/或FRG样蛋白编码核酸，和/或具有本公开内容的重组核酸的载体，还可以包含作为3’终止子序列的调节序列。本领域技术人员会容易地认识到可用于本公开内容的重组核酸的多种终止子。例如，本公开内容的重组核酸可以包含3’NOS终止子。此外，来自SHH1样蛋白编码核酸、SUVH2样蛋白编码核酸、SUVH9样蛋白编码核酸、DMS3样蛋白编码核酸、MORC6样蛋白编码核酸、SUVR2样蛋白编码核酸、DRD1样蛋白编码核酸、RDM1样蛋白编码核酸、DRM3样蛋白编码核酸、DRM2样蛋白编码核酸和/或FRG样蛋白编码核酸的天然终止子也可用于本公开内容的重组核酸。
植物转化方案以及将本公开内容的重组核酸引入植物的方案可取决于用于转化的靶植物或植物细胞的类型例如单子叶植物或双子叶植物而不同。将本公开内容的重组核酸引入植物细胞以及随后插入植物基因组中的合适的方法包括，不限于，显微注射(Crossway等人,Biotechniques(1986)4:320-334)、电穿孔(Riggs等人,Proc.Natl.AcadSci.USA(1986)83:5602-5606)、农杆菌介导的转化(美国专利号5,563,055)、直接基因转移(Paszkowski等人,EMBO J.(1984)3:2717-2722)和弹道颗粒加速(ballistic particleacceleration)(美国专利号4,945,050；Tomes等人1995."Direct DNA Transfer intoIntact Plant Cells via Microprojectile Bombardment,"in Plant Cell,Tissue,andOrgan Culture:Fundamental Methods,编辑Gamborg和Phillips(Springer-Verlag,Berlin)；和McCabe等人,Biotechnology(1988)6:923-926)。
另外，本公开内容的SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白可靶向至植物细胞内的特定细胞器。可通过为重组蛋白提供合适的靶向性肽序列而实现靶向。这类靶向性肽的实例包括，不限于，分泌型信号肽(用于分泌或细胞壁或膜靶向)、质粒转运肽、叶绿体转运肽、线粒体靶向性肽、液泡靶向性肽、核靶向性肽等(例如，参见Reiss等人,Mol.Gen.Genet.(1987)209(1):116-121；Settles和Martienssen,Trends Cell Biol(1998)12:494-501；Scott等人,J Biol Chem(2000)10:1074；以及Luque和Correas,J CellSci(2000)113:2485-2495)。
可根据常规方法(例如，参见McCormick等人,Plant Cell.Reports(1986)81-84.)使改造的植物生长成植株。然后使这些植株生长，并用相同的转化植株或不同的植株授粉，获得的杂合体具有期望的表型特征。可以生长两代或更多代以确保受试的表型特征被稳定保留并被遗传，且然后收获种子以确保已经实现期望的表型或其他性质。
降低植物中的基因表达的方法
本公开内容的另外的方面涉及通过利用SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白而降低植物中一种或更多种靶核酸例如基因的表达的方法。在一个方面，本公开内容提供了用于降低植物中一种或更多种靶核酸的表达的方法，所述方法通过提供包含本公开内容的一种或更多种重组多肽的植物，并在所述重组多肽藉以与本公开内容的一种或更多种靶核酸结合的条件下使所述植物生长，从而降低所述一种或更多种靶基因的表达。可以使用本文描述的并包含本公开内容的重组多肽的任何植物。
足以使植物中表达的重组多肽与本公开内容的一种或更多种靶核酸结合并降低所述一种或更多种靶核酸的表达的生长条件是本领域公知的，并且包括本文公开的任何合适的生长条件。通常，在足以表达本公开内容的重组多肽例如SHH1样蛋白、SUVH2样蛋白、SUVH9样蛋白、DMS3样蛋白、MORC6样蛋白、SUVR2样蛋白、DRD1样蛋白、RDM1样蛋白、DRM3样蛋白、DRM2样蛋白和/或FRG样蛋白，并且足以使所表达的重组多肽定位至植物的细胞核以与靶核酸结合并降低靶核酸的表达的条件下，使所述植物生长。一般来说，足以表达重组多肽的条件将取决于用于控制重组多肽的表达的启动子。例如，如果利用诱导型启动子，植物中重组多肽的表达将需要使植物在存在诱导物的情况下生长。
应理解，尽管已经结合其优选的具体实施方案描述了本公开内容，前述描述意欲举例说明本公开内容，并非限制本公开内容的范围。在本公开内容的范围内的其他方面、优势和修改对本公开内容所属技术领域的技术人员将是明显的。
提供以下实施例以举例说明所提供的实施方案，并且不意欲限制本公开内容的范围。
实施例
实施例1
以下实施例涉及拟南芥蛋白SHH1的表征和其参与促进RNA指导的DNA甲基化和基因沉默。
材料和方法
ChIP-seq、BS-seq和siRNA-seq文库构建和测序
使用Ovation Ultralow IL Multiplex System(NuGEN)产生ChIP-seq文库的第一重复(replicate)(NRPD1-Flag和Col)，同时使用Ovation Ultralow DR Multiplex System(NuGEN)产生第二重复(NRPD1-Flag、NRPD1-Flag；shh1和Col)。对于文库扩增步骤，两组ChIP-seq文库使用18个循环。如之前报道的(Cokus等人,2008)产生BS-seq文库。使用小RNATruSeq试剂盒(Illumina)除了在扩增步骤过程中使用15个循环之外遵循制造商的说明产生siRNA-seq文库。本研究中使用的野生型(Col)和nrpe1BS-seq文库以前已被公布(Zhong等人,2012)，且随后进行再分析。使用HiSeq 2000平台遵循制造商的说明(Illumina)以50bp的长度对所有文库进行测序。
读段的映射(mapping)和处理
使用标准Illumina pipeline对测序的读段进行碱基调用(base-called)。对于ChIP-seq和BS-seq文库，只保留全长50nt的读段，而对于siRNA-seq文库，剪掉读段的衔接子序列，并且如果读段的长度在18nt和28nt之间就保留它们。对于ChIP-seq和siRNA-seq文库，使用Bowtie(Langmead等人,2009)将读段映射到拟南芥基因组上(TAIR8)，并且尽管当将siRNA-seq文库针对每个文库的读段总数标准化时使用独特的和非独特的映射读段的总数，只保留独特地映射到基因组上的完全匹配序列用于进一步分析。对于BS-seq文库，使用用于Bowtie的BSseeker wrapper映射读段(Chen等人,2010)。对于ChIP-seq和BS-seq，将相同读段缩减为一个读段，而对于siRNA-seq，保留相同的读段。对于甲基化分析，使用未甲基化的叶绿体基因组作为非亚硫酸氢盐转化的背景甲基化的测量结果，如以前报道的(Cokus等人,2008)计算甲基化百分比。对于ChIP-seq的第二重复，对NRPD1-Flag和Col文库进行取样直到匹配较小文库(NRPD1-Flag；shh1文库)的读段总数。
DNA甲基化分析
对于在siRNA簇处的DNA甲基化的评价，仅考虑在被测定的各类中具有至少一个胞嘧啶(CG、CHG或CHH)的那些簇。为了通过甲基化水平的Mann-Whitney U检验计算不同亚类中的簇的甲基化改变的显著性水平(图1E)，对每个亚类中的簇的数目进行降低取样(downsample)至最小亚类(drm2/nrpe1亚类)以允许亚类之间的相当的显著性值。
siRNA簇的鉴定
以与以前公布的方法(Heisel等人,2008)类似的方式界定拟南芥基因组中的小RNA簇。简言之，将基因组分为200bp的格(bin)，并计算野生型(Col)文库的两个技术重复中不同的siRNA读段的每格平均覆盖度。使用该平均值测定野生型植物中在给定格处的不同读段的数目的显著性，呈现此类计数的Poisson分布。在R环境中，对每个格进行Poisson精确检验，并且认为在每个野生型技术重复中都具有小于1e-5的P值的格是簇。
界定了簇之后，使用Fisher精确检验对每个突变体和野生型(Col)文库进行读段(包括相同读段)计数之间的比较。对所得的P值进行Benjamini-Hochberg调整以评价FDR，并然后认为FDR<1e-10的突变体背景中减少的簇依赖于野生型功能的突变体蛋白。对于siRNA水平的箱形图分析，使用Col文库的第一技术重复作为Col siRNA水平的代表。为了通过siRNA水平的Mann-Whitney U检验计算不同基因型亚类中的簇的siRNA改变的显著性水平(图1D)，对每个亚类中的簇的数目进行降低取样至最小亚类(drm2/nrpe1亚类)以允许亚类之间的相当的显著性值。
NRPD1峰的鉴定
使用R包BayesPeak(Spyrou等人,2009；Cairns等人,2011)以鉴定与平行进行的配对Col ChIP-seq对照文库相比，NRPD1-Flag ChIP-seq文库中Pol-IV富集的区域。只保留在两个NRPD1-Flag ChIP-seq重复中都鉴定出的高得分峰(PP>0.999)(928个峰)，用于进一步分析。为了测定Pol IV峰与siRNA簇的重叠的目的，当峰与基因座重叠>＝1bp时，就被称为“重叠”。
为了将峰分类为SHH1-依赖性的、-非依赖性的或-增强性的，比较NRPD1-Flag与NRPD1-Flag；shh1 ChIP-seq文库之间的跨Pol IV峰的读段计数，并使用Fisher精确检验评价显著性。对所得的P值进行Benjamini-Hochberg调整以评价FDR。认为FDR<0.001的shh1文库中具有NRPD1信号损失的峰是SHH1-依赖性的。类似地，认为FDR<0.001的shh1中增加的信号的峰是SHH1-增强性的。认为未落入这些分类任一个中的峰是SHH1-非依赖性的。
蛋白制备
将编码SHH1的SAWADEE结构域(残基125-258)的基因克隆到自我修饰的载体中，所述载体将六聚组氨酸标签和酵母sumo标签融合到靶基因的N末端。将质粒转化到大肠杆菌(E.coli)菌株BL21(DE3)RIL(Stratagene)中。在37℃下培养细胞直至OD600达到0.8，且然后将培养基冷却至20℃，并添加0.2mM IPTG以诱导蛋白质表达过夜。首先使用HisTrap FF柱(GE Healthcare)纯化重组表达的蛋白。通过Ulp1蛋白酶裂解六聚组氨酸-sumo标签，并通过第二HisTrap FF柱而去除所述标签。使用Q FastFlow柱和Hiload Superdex G200 16/60柱(GE Healthcare)使用缓冲液(150mM NaCl，20mM Tris pH 8.0和5mM DTT)进一步纯化汇集的靶蛋白。为了制备Se-甲硫氨酸置换的蛋白，使用QuikChange定点突变试剂盒(Stratagene)将SAWADEE结构域的Leu200和Leu218突变为甲硫氨酸。使用与野生型蛋白相同的方案纯化Se-甲硫氨酸置换的SAWADEE蛋白。在肽合成设备上合成肽。
结晶
在4℃下使用坐滴气相扩散法通过混合1μl的浓度为5mg/ml的蛋白样品和1μl的储液器溶液(reservoir solution)(0.2M NH4F和20％PEG 3350)进行SAWADEE结构域的结晶，所述结晶在0.4ml储液器中平衡。在液滴中添加终浓度为7.6mM的4-环己基-1-丁基-β-D-麦芽糖苷(-4,Hampton Research)作为添加剂，这导致晶体质量的相当大的改进。在2天内出现薄板形晶体。为了产生SAWADEE结构域与修饰的H3肽的复合物的晶体，在4℃下以1:2的摩尔比混合SAWADEE结构域和肽，持续1小时。在与描述用于游离的SAWADEE蛋白的相同条件下，生长不同复合物的晶体。将所有晶体浸入补充有20％甘油的储液器溶液中，持续2分钟。然后将晶体安装在尼龙环上，用于衍射数据的采集。在芝加哥阿贡国家实验室先进光子源(APS)的NE-CAT光束线24ID-C处采集来自天然SAWADEE蛋白和其Se-甲硫氨酸置换的配对物的分别在锌峰和硒峰处的衍射数据。在纽约布鲁克海文国家实验室国家同步幅射光源(NSLS)的光束线X29A处采集与SAWADEE结构域结合的H3K9me3肽的复合物的数据。在APS 24ID-E处采集关于在与H3K9me2、H3K9me1和H3K4me1K9me1肽的复合物中的SAWADEE结构域的数据。使用HKL2000程序(Otwinowski等人,2011)处理所有结晶学数据。
结构确定和精修
使用在Phenix程序(Adams等人,2010)中执行的单波长反常散射(SAD)法，解析硒代甲硫氨酸置换的SAWADEE结构域的结构。使用Coot程序(Emsley等人,2010)进行模型建立，并使用Phenix程序进行结构精修。使用Phenix程序使用分子置换法解析游离状态的野生型SAWADEE结构域的结构。鉴定Zn2+离子并通过异常信号散射进一步证实。使用分子置换法以与天然蛋白相同的方案解析所有在与不同的修饰的H3肽的复合物中的SAWADEE结构域的结构。整个精修过程中，使用5％随机选择的反射(random chosen reflection)来计算自由R因子(free R factor)。使用Procheck程序(Laskowski等人,1993)分析立体化学的结构模型。使用Pymol程序(DeLano Scientific LLC)生成所有的分子图。
等温滴定量热法
6℃下在Microcal量热计ITC 200仪器上进行所有的结合实验。首先，将蛋白样品在4℃下相对于100mM NaCl、2mMβ-巯基乙醇和20mM HEPES的缓冲液pH 7.5透析过夜。然后稀释蛋白样品，并用相同缓冲液溶解冻干的肽。根据标准方案进行滴定，并使用Origin 7.0程序以1:1结合模型拟合数据。
修饰的肽阵列结合
在pENTR/TEV/D质粒(Invitrogen)中产生GST-SHH1 SAWADEE结构域(125-258aa)构建体，重组入pDEST 15质粒(Invitrogen)中，并转化到Rosetta 2(DE3)细菌细胞系(Novagen)中。通过在OD为0.6时每500mL添加500μL的1M IPTG，诱导蛋白表达，并在16℃下过夜培养培养物。诱导时，向培养基中补充500μL的500mM ZnSO4。然后如以前描述的(Johnson等人,2008)纯化GST融合蛋白，并透析到贮存缓冲液(50mM Tris pH 6.8，300mMNaCl，40％甘油，2mM DTT，0.1％tritonX-100)中。在以下条件下将纯化的GST-SHH1(125-258aa)蛋白用于探测MODifiedTM组蛋白肽阵列(Active Motif)：在25℃下在5％牛奶1x TBS溶液中封闭阵列45min，在25℃下在1x TBS-T溶液中洗涤三次，持续5分钟，并且然后在4℃下用在结合缓冲液(50mM HEPES pH7.5，50mM NaCl，5％甘油，0.4mg/mL BSA，2mM DTT)中的浓度为6.5μg/mL的GST-SHH1 SAWADEE探测过夜。然后如上洗涤阵列三次，并在25℃下用1:5000稀释的HRP缀合的GST抗体探测1小时。然后如上描述的洗涤阵列，并使用ECL Plus试剂盒(GE healthcare)显影。
株系、定点诱变、DNA印迹和蛋白质印迹
使用的多种以前表征的拟南芥RdDM突变体等位基因、互补SHH1-3xMyc-BLRP转基因株系和pSHH1::SHH1-3xMyc-BLRP构建体是以前描述的(Law等人,2011)。pol-iv和pol-v突变体分别对应于这些聚合酶的nrpd1和nrpe1亚基内的突变。使用QuikChange定点诱变试剂盒(Stratagene)在pSHH1::SHH1-3xMyc-BLRP构建体中产生基于结构的突变，并经由蘸花法将其转化到shh1-1突变体背景中。使用花组织在Col和RdDM突变体系中进行siRNA-seq和ChIP-seq实验，并使用10天龄的幼苗进行BS-seq实验。使用来自T1代的同一个体株系的组织并使用以前描述的探针(Johnson等人,2008)和抗体(Law等人,2010)进行DNA印迹和蛋白质印迹实验。分别使用来自多个pSHH1::SHH1-3xMyc-BLRP转基因纯合的T3植株的花组织或10天龄的幼苗，在SAWADEE结构域点突变体系中进行siRNA-seq和BS-seq实验。使用来自shh1突变体等位基因纯合的F1植株的花组织，在多种SAWADEE结构域点突变体背景中进行Pol IV ChIP实验和免疫共沉淀实验。
结果
为了研究SHH1在基因组范围内对RdDM通路中的作用，产生了野生型Col植株、shh1突变植株和一些其它RdDM突变体的siRNA谱，用于进行比较。在野生型植株中，界定了～12,500个siRNA簇，代表全部独特地映射的24nt siRNA的84.2％。与以前的发现结果一致，这些siRNA的81.4％是Pol-IV依赖性的(Mosher等人,2008；Zhang等人,2007)(图1A)。在shh1突变体中减少的siRNA簇的分析证实了SHH1是siRNA水平的主要调控因子，影响了44％的Pol-IV依赖性簇，这代表了全部24nt siRNA的大多数，包括在两个下游RdDM突变体(drm2和pol-v)中减少的簇的大多数(图1B)。在这些突变体中减少的siRNA簇的重叠形成四个主要亚类(称为仅pol-iv、shh1、shh1/drm2/pol-v和drm2/pol-v；图1B)，将其用于后续分析。仅依赖于Pol-IV的簇比还依赖于SHH1、DRM2和Pol-V的那些在近着丝粒异染色质(pericentromeric heterochromatin)中更加丰富(图1C)，表明在常染色质臂和近着丝粒异染色质中，不同的机制可能控制siRNA产生。
在shh1突变体中，SHH1-依赖性簇(shh1和shh1/drm2/pol-v亚类)处的siRNA水平被降低到接近于零，而SHH1-非依赖性簇处的siRNA水平经历很少至没有变化(图1D)。这些结果证明，SHH1是基因座特异性的RdDM组分，其在RdDM基因座的一个大子集处具有强烈的影响。值得注意的是，两个下游RdDM突变体(drm2和pol-v)对在还需要SHH1的簇(shh1/drm2/pol-v亚类)处的siRNA水平具有最强的影响，并且这些相同的簇在产生最高siRNA的簇之中，并且对应于基因组中CHH甲基化的最高水平(Cokus等人,2008)(图1D、1E)。总之，这些发现结果表明，SHH1和下游RdDM突变体特异性地趋同(converge)以控制在RdDM的最具活性的位点处的siRNA水平。
使用全基因组亚硫酸氢盐测序(BS-seq)，我们评价了在显示出降低的siRNA水平的基因座处的DNA甲基化水平，并发现和SHH1与Pol-IV的相互作用一致，SHH1是上游RdDM组分；shh1突变体只影响其中siRNA水平降低的位点处的DNA甲基化(图1E)。此外，如对上游RdDM组分预测的，在shh1突变体中存在的残留的siRNA表现出靶向一些甲基化，这与下游突变体drm2和pol-v相反，其将甚至大量保留siRNA的位点处的DNA甲基化降低到接近pol-iv水平(图1E)，这可能是由于不能利用siRNA靶向DNA甲基化的缘故。
在对应于siRNA簇的shh1/drm2/pol-v和drm2/pol-v亚类的基因座处，所观察到的siRNA的损失伴随着相应较大的DNA甲基化损失(图1E)。但是，在仅pol-iv和shh1亚类处，siRNA的较大损失伴随着相对较小的DNA甲基化损失。不希望囿于理论，认为对于该发现结果的可能的解释是其它DNA甲基化通路在对应于仅pol-iv和shh1siRNA簇的位点处更加有活性。除了RdDM通路之外，DNA甲基化还受两个维持性甲基转移酶通路控制(Law和Jacobson,2010)：作用是维持CG甲基化的DNA甲基转移酶1(MET1)通路，并且与一些H3K9组蛋白甲基转移酶一起作用以维持CHG和一些CHH甲基化的染色质甲基化转移酶3(CMT3)通路(Cao等人,2003)。与甲基转移酶冗余的这个见解一致，具有减少的siRNA簇的仅pol-iv和shh1亚类表现出最高水平的CMT3占据(Du等人,2012)(图1F)，表明在不存在功能性RdDM通路的情况下，CMT3通路能够在这些基因座处将DNA甲基化维持在接近野生型的水平。相反，在RdDM突变体中表现出大幅度DNA甲基化损失的shh1/drm2/pol-v和drm2/pol-v亚类，表现出更低水平的CMT3富集(图1F)，并且更为高度地且精确地富集Pol-V聚合酶(Zhong等人,2012)(图1F、1G)，表明它们主要被RdDM通路靶向。
通过最大Pol-IV亚基NRPD1的加Flag标签形式的染色质免疫沉淀(Law等人,2011)以及随后的高通量测序(ChIP-seq)，确定野生型和shh1突变体背景中Pol-IV占据的全基因组谱。与Pol-IV依赖性siRNA簇的谱一致，Pol-IV在近着丝粒异染色质处以及在界定的siRNA簇的亚类处广泛富集(图2A、2B)。在shh1突变体背景中，Pol-IV水平大幅度降低，或在shh1依赖性siRNA簇处被特异性消除(图2A)，进一步支持了ChIP-seq谱的生物相关性，并证实了shh1突变体的siRNA减少的表型是由于改变的Pol-IV染色质缔合。在shh1非依赖性siRNA簇处，Pol-IV水平与siRNA水平类似，在shh1突变体中未减少(图2A)，表明Pol-IV靶向这些基因座需要替代性的机制。
除了评价Pol-IV跨受影响的siRNA簇亚类的富集水平，使用多重ChIP-seq数据集界定了928个可重复的高可信度的Pol-IV峰。这些峰针对siRNA和DNA甲基化被富集，并且与仅pol-iv和shh1簇相比优选与产生高siRNA的shh1/drm2/pol-v或drm2/pol-v簇重叠(P<2.2e-16,Fisher精确检验)，表明ChIP程序优选鉴定了其中Pol-IV最有活性的位点。在928个界定的Pol-IV峰中，观察到可变水平的SHH1-依赖性，提示将这些峰分为三个种类，SHH1-非依赖性的，SHH1-依赖性的和SHH1-增强性的。在shh1突变体中，DNA甲基化和siRNA水平在SHH1-依赖性的位点处降低，并且在被界定为SHH1-非依赖性的位点处为更小的程度的降低。但是，siRNA和Pol-IV水平在shh1突变体中的SHH1-增强性位点处增加，表明Pol-IV重新分布至shh1突变体中的这些位点。这些SHH1-增强性位点在Pol-IV峰中是独特的，因为它们表现出非常低水平的Pol-V富集，这能够解释在野生型植株中的这些位点处观察到的相应低水平的CHH甲基化。与SHH1-依赖性的siRNA簇的分析一起，这些发现结果证实了SHH1在促进RdDM的最活跃位点的子集处的Pol-IV染色质缔合中起到至关重要的作用。因此，SHH1代表已知影响Pol-IV的靶向作用的第一个因子，控制了RdDM的起始。
为了深入研究SHH1藉以促进Pol-IV靶向作用的机制，研究了它的SAWADEE结构域的功能，所述SAWADEE结构域是具有未知功能的植物特有的结构域(Mukherjee等人,2009)。图10中示出了来自不同物种的SHH1蛋白的序列比对。使用活性基序修饰的肽阵列测试了SAWADEE结构域结合修饰的组蛋白尾部的能力。该测定表明，SAWADEE结构域具有对H3K9甲基化的偏好性，但是还受到H3K4残基的甲基化状态的影响，只有未修饰的或H3K4me1修饰被容许。为了证实这些结果，使用修饰的组蛋白尾肽进行等温量热法(ITC)实验(图3A、3B)。这些分析表明，SAWADEE结构域在其与所有三种H3K9甲基化状态(me1、me2和me3)结合的能力方面是非常独特的，具有非常相似的亲和力，Kd≈2μM，所述亲和力比使用未修饰的H3肽所观察到的高大约17倍(图3A)。ITC实验还证实，尽管SAWADEE结构域将结合包含H3K4me1修饰的H3K9me2肽，H3K4me2或H3K4me3修饰的存在导致降低的结合亲和力。最后，使用修饰的肽的ITC实验证实了SHH1 SAWADEE结构域对于H3K9甲基化的特异性，所述修饰的肽对应于在核心组蛋白蛋白质的N末端尾部的所有其它已知的甲基化的赖氨酸残基(图3B)。总之，这些结合研究证实，SAWADEE结构域是一种新的染色质结合模块，其探测H3尾部的K4和K9位置，并且特异性结合抑制性H3K9甲基修饰。
与体外观察到的SHH1 SAWADEE结构域的结合特异性一致，对于H3K9me2，SHH1-依赖性的Pol-IV ChIP-seq峰被富集(图3B)。对于H3K4甲基化，Pol-IV ChIP-seq峰被耗尽。
为了确定SHH1 SAWADEE结构域识别甲基-赖氨酸的模式，解析了游离状态的或在与修饰的H3尾部的复合物中的该结构域的晶体结构。在游离状态中，SHH1 SAWADEE结构域采用串联Tudor结构域样折叠，其在Tudor2亚结构域中包含独特的锌结合基序(图3D)，使得SHH1成为串联Tudor结构域折叠的新亚类的创始成员(Bian等人,2011)。在该结构中，锌离子被高度保守的半胱氨酸和组氨酸残基配位(Mukherjee等人,2009)。DALI检索表明，尽管仅共享11.8％的序列同一性，SAWADEE结构域的总体结构类似于UHRF1串联Tudor结构域，具有r.m.s.d.为(Holm和Rosenstrom,2010；Nady等人,2011)。该发现结果证实了虽然SAWADEE结构域的序列是植物特有的，但是它的折叠在真核生物中高度保守的。
还解析了在与H3K9me1、H3K9me2和H3K9me3肽的复合物中的SHH1 SAWADEE结构域的结构，并且所有三种肽以相似的方式被结合。进一步分析了解析的具有H3(1-15)K9me2肽的结构(图4A)。该肽定向性地结合在两个Tudor亚结构域之间的沟中，与两个亚结构域形成接触(图4A、4B、4C)。SAWADEE结构域的游离的和H3K9me2结合的结构能够被很好地叠合(图4D)，证实了配体结合后SAWADEE结构域中不存在显著的构象变化，这一发现与报道的UHRF1的情况(Nady等人,2011)不同。
在SHH1 SAWADEE结构域中，存在两个口袋，所述两个口袋与被结合的肽的未修饰的K4和K9me2侧链形成分子间相互作用(图4E、4F)。未修饰的H3K4侧链插入到由来自Tudor1和Tudor2亚结构域二者的残基形成的界面口袋中。在该口袋中，经由与Glu130和Asp141的侧链的分子间氢键和静电相互作用，稳定K4侧链(图4E)。H3K9me2侧链插入由Tudor 1亚结构域的残基形成的疏水性芳香族笼中(图4F)，其中通过阳离子-π相互作用以与之前报道的甲基化的赖氨酸结合模块的那些(Taverna等人,2007)的类似方式稳定所述H3K9me2侧链。H3K9me3-SAWADEE和H3K9me1-SAWADEE复合物也将甲基化的赖氨酸置于相同的芳香族笼中。不希望囿于理论，认为SAWADEE结构域与所有三种H3K9甲基化状态等同结合的能力，可以被结构观察结果良好地解释：通过常见的与芳香族笼的疏水性相互作用，甲基化的赖氨酸识别性芳香族笼能够容纳H3K9me2和H3K9me3侧链二者，导致这两个甲基化状态之间缺乏差异。在H3K9me1复合物中，虽然更低的赖氨酸甲基化状态具有降低的与芳香族笼的疏水性相互作用，His169的侧链经历了小的但是显著的构象变化以与K9me1铵质子形成氢键，从而有助于结合亲和力的恢复。这种对K9甲基化状态的特异性的缺乏，与观察到的对UHRF1的串联Tubor结构域的更高水平的甲基化特异性相反，所述UHRF1的串联Tubor结构域具有稍微更宽的芳香族笼结合口袋，所述芳香族笼结合口袋包含Phe和Tyr残基的不同组合。不希望囿于理论，认为这导致不同的形状互补性需求。结构分析指示了在串联Tudor结构域折叠中非常精细的变化如何能够导致甲基-赖氨酸特异性的精细转变。
还解析了在与H3(1-15)K4me1K9me1肽的复合物中的SAWADEE结构域的结构。总体来说，该结构类似于具有H3K9me2肽的结构，在相同的K4结合口袋中容纳K4me1。但是，甲基基团与Leu201形成稳定的疏水性相互作用，取代了未甲基化的K4的铵质子与Glu130侧链之间形成氢键(图4G)。由于该K4结合口袋相对封闭和窄，K4的更高的甲基化状态可能会引起空间冲突和/或干扰所有氢键相互作用。不希望囿于理论，认为这解释了观察到的亲和力的降低。
为了测试观察到的SHH1 SAWADEE结构域的甲基-H3K9结合活性的生物学显著性，在两个赖氨酸结合口袋中以及锌结合基序中产生点突变。分析了这些突变对体内DNA甲基化、siRNA水平和Pol-IV募集的影响。具体地，将这些点突变工程化到SHH1-3xMyc-BLRP-构建体中，并转化到shh1突变背景中。通过DNA印迹评价了良好表征的基因座MEA-ISR处的DNA甲基化水平，并通过BS-seq实验评价基因组范围的DNA甲基化水平(图4H)。添加了野生型SHH1-3xMyc-BLRP转基因恢复的DNA甲基化，但是，尽管以与野生型SHH1-3xMyc-BLRP蛋白相当的水平表达，具有在H3K9或H3K4口袋中的突变的构建体不能够完全弥补在shh1突变体中观察到的甲基化缺陷(图4H)。锌配位残基中的突变导致几乎不可检测的水平的蛋白，并因此未被进一步表征。
与shh1无效突变体相似，在SHH1赖氨酸结合口袋突变体中的DNA甲基化缺陷在受影响的siRNA簇的shh1/drm2/pol-v亚类中是最显著的(图4H)，并且与它们的位置和预测的对SHH1 SAWADEE结构域的结合亲和力的贡献一致，F162AF165A和D141A突变体表现出更强的DNA甲基化缺陷(图4H)。与观察到的DNA甲基化的减少相一致，经由siRNA-seq实验对赖氨酸结合口袋突变体的siRNA水平的评价表明F162AF165A和D141A突变体的类似模式的缺陷，再次表现出更强的表型(图4I)。为了确定观察到的siRNA和DNA甲基化的损失是否反应Pol-IV在染色质处的活性的缺陷，在SAWADEE结构域点突变体背景中进行Pol-IV ChIP实验。所有四个点突变体表现出降低水平的Pol-IV在两个生物重复中的占据(图4J)。另外，免疫共沉淀表明，SHH1 SAWADEE结构域点突变体仍然能够与Pol IV相互作用，证明了SHH1与PolIV复合物之间的相互作用不依赖于它的H3K9me结合活性。总之，这些发现结果表明，在K4和K9结合口袋中的残基对于SHH1的体内功能是至关重要的，并且证实了通过SHH1的甲基-H3K9结合在Pol IV与染色质缔合的水平的作用。
发现H3K4结合口袋对于SHH1的体内功能是重要的。在不存在H3K9甲基化的情况下SHH1 SAWADEE不会结合H3K4甲基化，并且向K4添加甲基基团不会给予任何另外的结合亲和力。不希望囿于理论，认为在从甲基化的H3K9残基往回5个残基的位置处仅存在赖氨酸是SAWADEE结构域结合所必需的。这类双赖氨酸读取能够用来帮助确认，SAWADEE结构域仅在当赖氨酸甲基化存在于H3尾部的K9位置处时才结合赖氨酸甲基化，这与甲基化的赖氨酸在H3尾部上或甚至在不同组蛋白或非组蛋白蛋白质上的不同位置处相反。使用具有H3K4A突变的H3尾部，在存在或不存在H3K9me2修饰的情况下，进行ITC实验。SAWADEE结构域以比与H3K9me2肽的约30倍更弱的亲和力结合H3K4AK9me2肽。此外，SHH1 SAWADEE结构域以比与野生型H3尾部的更弱的亲和力结合H3K4A肽，证实了K4残基独立于K9残基的甲基化状态而促成结合。
总之，这些体内和体外分析证实了SHH1 SAWADEE结构域在K4和K9位置处探测H3尾部，并且对于存在于转座子和其他重复DNA元件处的组蛋白修饰的组合，即未修饰的H3K4和甲基化的H3K9具有非常高的选择性。虽然在基因组范围内，H3K9甲基化与H3K4甲基化呈反相关(Zhang等人,2009)。不希望囿于理论，认为SAWADEE结构域对更高等级的H3K4甲基化的排斥能够用来允许与H3K4甲基化相关的转录，目的是以发育特异性或基因座特异性的方式克服DNA甲基化和相关的抑制性H3K9甲基修饰。同样，SAWADEE结构域的特异性能够抑制在体内被甲基化的基因处的siRNA的产生，所述被甲基化的基因包含CG甲基化和H3K4甲基修饰，但是缺乏CHG和CHH甲基化以及siRNA(Cokus等人,2008；Zhang等人,2009；Zhang等人,2006)。
综上所述，证实了SHH1是一种新的染色质结合蛋白，其在RdDM中发挥功能以使Pol-IV能够在最活跃地靶向的基因座处的募集和/或稳定，以促进siRNA生物发生。不希望囿于理论，认为SHH1与抑制性组蛋白修饰结合的发现结果，以及SHH1是在与下游RdDM突变体类似的一组基因座处的Pol-IV染色质缔合所必需的观察结果，能够解释之前观察到的自我加强环路(self-reinforcing loop)，其中下游RdDM突变体是从一个亚组的基因座产生完全水平的siRNA所必需的(Zilberman等人,2004；Xie等人,2004；Li等人,2006；Pontes等人,2006)，因为已经表明下游RdDM突变体能够引起RdDM基因座处的DNA甲基化和H3K9甲基化二者的降低(Zilberman等人,2003)。
实施例2
以下实施例涉及拟南芥蛋白SUVH2和SUVH9的表征和其参与促进RNA指导的DNA甲基化和基因沉默。
引言
所有DNA甲基化和很多非CG甲基化的维持的确立涉及RNA指导的DNA甲基化(RdDM)通路(Aufsatz等人,2002；Pelissier和Wassenegger,2000)。不希望囿于理论，认为在该通路中存在两个主要步骤，所述两个主要步骤被认为靶向DNA甲基转移酶、结构域重排甲基转移酶2(DRM2)(Cao和Jacobsen,2002)。第一个上游步骤涉及通过RNA聚合酶IV(Pol IV或NRPD)、RNA指导的RNA聚合酶2(RDR2)和DICER样3(DCL3)的协同作用合成24个核苷酸的小干扰RNA(siRNA)(Pontier等人,2005)。第二个下游步骤涉及通过RNA聚合酶V(Pol V或NRPE)在DDR复合物(DRD1，一种SWI/SNF2染色质重塑酶(remodeler)；DMS3，一种染色体架构蛋白；RDM1，未知功能)的帮助下，产生支架转录物。不希望囿于理论，然后认为负载有24个核苷酸siRNA的ARGONAUTE 4(AGO4)与Pol V转录物结合，并且以未知方式发挥作用以指导DRM2至DNA用于甲基化(Law等人,2010；Pikaard等人,2008；Wierzbicki等人,2009)。
该实施例证实了Pol V转录以及其与染色质的稳定结合依赖于基因组范围内的SUVH2/SUVH9。这些Pol V结合位点在DNA甲基化且在许多情况下，在组蛋白H3K9甲基化的情况下被富集。此外，表明将SUVH2拴系至未甲基化的表观等位基因fwa-4导致FWA基因的DNA甲基化和沉默的确立。该甲基化的确立与Pol V的募集一致，但是与组蛋白甲基化的立即富集不一致，表明组蛋白甲基化对于DNA甲基化的确立是次要的。还显示出了SUVH9的晶体结构，从而明确了该酶家族中的组成结构域的折叠和相对朝向。在N末端附近的双螺旋束位于SRA和pre-SET/SET结构域之间，并与SRA和pre-SET/SET结构域相互作用。观察到不完全形成的SAM辅因子结合口袋以及在SUVH9的结构中不存在组蛋白肽底物结合裂缝，表明在该酶中不存在post-SET结构域。不希望囿于理论，认为这可以解释其缺乏体外甲基转移酶活性。这些结果表明，SUVH2和SUVH9在将Pol V募集至染色质中发挥作用，为PolV提供了一种读取表观遗传标志以指导其活性的方法。
材料和方法
生物材料
使用的所有植株都具有拟南芥哥伦比亚型(Col-0)登记，WT指亲本植株。之前描述了nrpe1-12 T-DNA(SALK_033852)、suvh2suvh9双突变体株系、suvh4suvh5suvh6三突变体株系(Johnson等人,2008；Pontier等人,2005)。将NRPE1-FLAG转基因植株(El-Shami等人,2007)与suvh2suvh9双突变体杂交，并鉴定了纯合的F2植株。从哥伦比亚背景中的met1分离群中分离fwa-4表观等位基因。
Pol V转录和染色质免疫沉淀
使用TRIzol试剂(Invitrogen)从花中分离总RNA，并用于使用SuperScript III(Invitrogen)合成第一链cDNA。在MxPro3000 qPCR仪(Stratagene)中遵循制造商的说明使用SYBR Green SuperMix(Bio-Rad)进行实时PCR。使用1-2克的3周龄的植物或花进行ChIP，并如以前描述的(Zhong等人,2012)在体内或体外用1％甲醛交联。从Upstate(#07-450)获得抗-H3K9me1，从Abcam(ab1220)获得抗-H3K9me2，并且抗-NRPE1来自Craig Pikaard的惠赠。
文库产生
使用来自NEB的配对末端试剂和来自Illumina的衔接子产生组蛋白ChIP-seq的文库。使用NuGen multiplex试剂盒产生NRPE ChIP-seq的文库。如以前报道的(Feng,2011)产生BS-seq文库。使用HiSeq 2000平台遵循制造商的说明(Illumina)以50bp的长度对所有文库测序。
比对
分别使用BS-seeker和bowtie将亚硫酸氢盐-Seq和ChIP-seq比对到TAIR8版本的拟南芥参考基因组上，允许多达2个错配。只考虑独特映射的读段。标准化ChIP-seq文库，使得所有文库会包含相等数目的读段。将跨NRPE1位点的NRPE1-Flag的富集(Zhong等人,2012)针对在哥伦比亚中的Flag ChIP(阴性对照)标准化。将跨NRPE位点的组蛋白甲基化针对确定包含基线水平的组蛋白甲基化的80个位点甲基化。这些精心挑选的位点在明确可以映射的区域，但是还具有低组蛋白甲基化的其他特征性的标志，例如接近基因和低DNA甲基化。
Zn指构建体
如Segal等人(Segal等人,2003)中的描述的设计包含6个Zn指的肽，并克隆到pUC57中，所述肽的两端为Xho I位点(Genewiz)。切下该Xho I片段，并插入到pENTR-SUVH2构建体(Johnson等人,2008)中的位于BLRP肽和HA标签之间的唯一Xho I位点中，所述pENTR-SUVH2构建体编码由内源性SUVH2启动子驱动的HA-加标签的SUVH2。然后将该ZF-SUVH2重组到JP726(Johnson等人,2008)中，并使用农杆菌介导的转化将其引入fwa-4中。使用BASTA筛选转化的株系。
开花时间
通过计数直至最后开花的所有莲座叶和茎生叶，确定在短白昼的条件下生长的植物的开花时间。确定20-30个植株的平均叶片数。
蛋白质制备
将N-末端截短的SUVH9(残基134-650)克隆到pFastBacHT B载体(Invitrogen)中，其将后面带有TEV裂解位点的六组氨酸标签融合至靶基因的N-末端。将质粒转化到大肠杆菌菌株DH10Bac(Invitrogen)中，以产生杆粒。通过遵循标准Bac-to-Bac方案(Invitrogen)用杆粒转染Sf9细胞，产生杆状病毒。随后将收获的病毒用于感染悬浮的Hi5细胞，用于重组蛋白表达。使用镍亲和层析柱(GE Healthcare)首先纯化重组蛋白。通过TEV蛋白酶裂解六组氨酸标签。使用Q sepharose柱和Superdex G200凝胶过滤柱(GE Healthcare)进一步纯化靶蛋白。将纯化的蛋白浓缩至15mg/ml，并保存于-80℃。
结晶
晶体筛选之前，在4℃下将SUVH9蛋白与推测的辅因子S-腺苷-L-同型半胱氨酸(SAH)以1:3的摩尔比混合，或将SUVH9蛋白与SAH和组蛋白H3(1-15)肽以1:3:3的摩尔比混合，持续2小时。在20℃下使用悬滴气相扩散法，通过混合1μl的浓度为11.5mg/ml的蛋白样品和1μl储液器溶液并对500μl储液器溶液平衡，进行SUVH9的结晶。在0.2M硫氰酸钾、0.1MBis-Tris丙烷pH 7.5和20％游离形式的PEG 3350的条件下，以及在存在SAH的情况下或在存在SAH和H3(1-15)肽的条件下，进行SUVH9结晶。2周内出现方形晶体。将所有晶体浸入补充有15％甘油的储液器溶液中，并快速冷却到液氮中，用于衍射数据的采集。在纽约Brookhaven国家实验室(BNL)的国家同步幅射光源(NSLS)的光束线X29A处在锌峰波长处采集衍射数据。使用程序HKL2000(Otwinowski和Minor,1997)对数据进行索引、整合和进一步依比例处理。
结构确定和精修
用在程序Phenix(Adams等人,2010)中执行的单波长反常散射法，解析游离状态的SUVH9的结构。使用程序Coot(Emsley等人,2010)进行模型建立，并使用程序Phenix(Adams等人,2010)进行结构精修。在整个精修过程中，使用5％随机选择的反射来计算自由R因子。使用程序Procheck(Laskowski等人,1993)分析立体化学的结构模型。数据表明，在存在SAH的情况下以及在存在SAH和H3(1-15)肽的情况下，SUVH9的晶体事实上是游离形式SUVH9的晶体。使用程序Pymol(DeLano Scientific LLC)生成所有的分子图。
结果
通过Pol V合成非编码RNA对RdDM中的下游步骤是重要的。为了确定SUVH2/SUVH9在这些转录物的合成之前或之后是否起作用，在若干表征的位点(图5A中所示的IGN22和P6)处测定Pol V转录(Wierzbicki等人,2008；Zhong等人,2012)。发现双突变体suvh2suvh9使Pol V转录物降低至与Pol V突变体nrpe1相同的程度(图5A)。这些结果表明，SUVH2/SUVH9是Pol V活性或Pol V募集至染色质所需要的。使用Flag-加标签的Pol V的染色质免疫沉淀(ChIP)观察到，与野生型中的6倍富集相比，发现在suvh2 suvh9双突变体中在IGN5和IGN22处只有背景水平的Pol V结合(图5B)。通过下一代测序进一步分析ChIP数据(ChIP-seq)，并发现Pol V在所有之前鉴定的位点(Zhong等人,2012)处的结合在suvh2suvh9背景中显著降低(图5C、5D)。因此，SUVH2和SUVH9不仅是Pol V活性所需要的，还是PolV与染色质的稳定缔合所需要的。
为了确定多个突变体中的Pol V结合位点处的DNA甲基化水平，进行全基因组亚硫酸氢盐测序。发现suvh2 suvh9突变体株系使CHH甲基化减少了85％，这接近在nrpe1突变体株系中测量的甲基化降低(图5E)。这些相同的突变体株系使CHG甲基化减少了多于50％，强调了RdDM通路也部分负责CHG甲基化(Law和Jacobsen,2010)。这些结果证实了在基因组范围内的Pol V位点处的RNA指导的DNA甲基化依赖于SUVH2和SUVH9。发现在Pol V位点处的甲基化还部分依赖于SUVH4/SUVH5/SUVH6/CMT3通路，因为suvh4suvh5suvh6三突变体使在PolV位点处的CHG甲基化减少了大约75％(图5E；在cmt3突变体中观察到类似的降低)。
由于SUVH2/SUVH9与SUVH4/SUVH5/SUVH6在同一SET蛋白质家族中，通过ChIP-seq使用对H3K9me1和H3K9me2特异性的抗体，分析基因组范围内的组蛋白H3K9甲基化。观察到非CG DNA甲基化与H3K9me1和H3K9me2之间的高度相关性。双突变体suvh2 suvh9未显著影响基因组范围内的H3K9me1或H3K9me2的水平，而三突变体suvh4 suvh5 suvh6的H3K9me1和H3K9me2都具有显著降低。映射跨Pol V结合位点的H3K9me1和H3K9me2，并发现二者相对于基因组平均值均被富集，虽然发现H3K9me1处于比H3K9me2更高的水平。在suvh4 suvh5suvh6三突变体植株中，在所有Pol V位点处的H3K9me1都被降低至背景水平，而suvh2suvh9双突变体导致组蛋白甲基化的显著损失(图5F)。任何突变体中的H3K9me2都未显著改变。
不希望囿于理论，认为虽然在suvh2 suvh9双突变体中观察到的损失可能是直接的，但是若干证据线索支持组蛋白甲基化的这些降低是降低的DNA甲基化的次级结果。首先，在一些位点处，H3K9me1是一片较大的异染色质的部分，并且不受suvh2 suvh9的影响，而在其他位点处，DNA甲基化和H3K9me1均损失。此外，发现第三类位点不具有可检测的H3K9me1，但是其具有SUVH2/SUVH9依赖性的DNA甲基化。第二，在另一个RdDM突变体rdr2中，在这些相同的位点处观察到组蛋白甲基化的类似的减少。因此，可能的是，SUVH2/SUVH9发挥功能以募集Pol V，导致RdDM，其然后募集活性组蛋白甲基转移酶SUVH4、SUVH5和SUVH6。这些酶通过它们的SRA结构域结合甲基化的DNA，并甲基化缔合的组蛋白。不希望囿于理论，认为最可能的是，suvh2 suvh9双突变体中H3K9甲基化的损失是RNA指导的DNA甲基化的损失的间接结果。
为了直接测试SUVH2/SUVH9的作用是否可以足以靶向RNA指导的DNA甲基化，工程化了特定的锌指(ZF)，所述锌指(ZF)将SUVH2拴系至FWA的未甲基化的表观等位基因fwa-4(fwa-4是哥伦比亚中的未甲基化的FWA表观等位基因，fwa-1是Landsberg erecta生态型中的未甲基化的表观等位基因)。FWA基因编码同源结构域转录因子，所述同源结构域转录因子由于其启动子中的DNA甲基化而通常被沉默(Soppe等人,2000)。FWA表观等位基因损失了全部DNA甲基化，导致FWA的异位表达和可遗传的晚开花(Kakutani,1997；Soppe等人,2000)。通常参与靶向DNA甲基化的siRNA仍然存在于FWA表观等位基因中，但是似乎不能指导下游因子以甲基化启动子(Chan等人,2006)。不希望囿于理论，认为在一个模型中，虽然存在siRNA，但是丢失了转录它的Pol V支架。FWA启动子区包含两个小的同向重复段，其后是两个较大的重复段(Kinoshita等人,2007)。设计包含六个ZF的肽，以识别在两个小的重复段的每一个中可见的序列(CGGAAAGATGTATGGGCT)(SEQ ID NO:141)(Kolb等人,2005；Segal等人,2003)。然后将该肽的编码区插入到由内源性SUVH2启动子驱动的HA-加标签的SUVH2转基因中(ZF-SUVH2)，并使用农杆菌介导的转化将其引入fwa-4株系中(图6A)。
在T1植株中，大约75％的ZF-SUVH2转化株与亲本fwa-4株系相比开花早。不包含Zn指的HA-加标签的SUVH2(HA-SUVH2)对照株系的转化株在与fwa-4亲本相同的时间开花，表明该作用是ZF-SUVH2融合物特有的。对包含ZF-SUVH2或HA-SUVH2的T1植株执行至T2代，并确定开花时间(图6A、6B)。包含ZF-SUVH2的株系开花仅比哥伦比亚(WT)对照稍晚，而不包含Zn指的HA-SUVH2在与fwa-4亲本大约相同的时间开花。这些结果表明，ZF-SUVH2事实上靶向FWA启动子，并且引起转录抑制。
使用亚硫酸氢盐测序分析FWA启动子区域中的DNA甲基化。在野生型株系中，检测到大区域的DNA甲基化，具有特别高水平的CG甲基化(图6D)。在fwa-4中，该区域完全没有DNA甲基化。在fwa-4中包含ZF-SUVH2的三个独立的T1株系中，观察到不同于WT的甲基化谱。在所有三个序列环境中，在紧邻Zn指结合位点周围的DNA甲基化处于高水平。然后DNA甲基化随着下游被转录区域而逐渐减少(图6E)。在对照HA-SUVH2 T1植株中，在FWA处未观察到DNA甲基化。这些结果表明，使用ZF将SUVH2靶向至FWA足以引起DNA甲基化和基因沉默。
为了确定Pol V是否存在于fwa-4表观突变体中的FWA处，在野生型、nrpe1和fwa-4株系的ChIP实验中使用针对NRPE1的内源性抗体。在野生型和fwa-4株系中观察到在两个已知的Pol V位点IGN5和IGN22处的Pol V的富集，但是在nrpe1突变体植株中则未观察到(图7A)。当检查FWA基因座时，在野生型中观察到在启动子和转录区二者处的富集，但是在nrpe1或fwa-4中则未观察到(图7A)。因此，当Pol V在野生型中处于其DNA甲基化的和沉默的状态时Pol V存在于FWA位置处，但是当Pol V在fwa-4表观等位基因中处于未甲基化的状态时则不存在于FWA位置处。为了确定ZF-SUVH2与FWA启动子的结合是否募集PolV，在fwa-4中包含ZF-SUVH2的T2株系中进行NRPE1 ChIP。与亲本fwa-4株系不同，在存在ZF-SUVH2的情况下，观察到Pol V在FWA处的富集(图7A)，表明ZF-SUVH2靶向作用起作用以募集Pol V或稳定在FWA处的Pol V。
还测定了不同株系中FWA的组蛋白甲基化状态。在野生型株系中，观察到H3K9me1在FWA启动子区中的轻微富集以及在FWA转录区中的4倍富集(图7B)。在fwa-4株系中，未观察到富集。未检测到在早开花的ZF-SUVH2/fwa-4 T1植株中的H3K9me1的富集(图7B)。但是，在来自这些植株的下一代(T2)中，观察到H3K9me1的轻微富集，启动子区比转录区稍强(图7C)。这些结果表明，虽然在第一代中ZF-SUVH2立即募集Pol V和DNA甲基化，组蛋白甲基化未立即恢复，但是在将植株同系交配获得另一代之后会累积。
为了获得对SUVH2/SUVH9功能的另外的研究，进行了两个蛋白质的结构分析。通过蛋白质结晶，解析了N-末端截短的SUVH9构建体(残基134-650)的结构，所述结构代表拟南芥SUVH家族包含SRA-SET盒的蛋白质的第一结构视图。预测SUVH9的前130个残基是无序的并且与任何已知的结构域不具有同源性，同时其余的残基包含SUVH9的所有已知的功能性结构域(SRA、pre-SET和SET结构域)(图8A)。使用单波长反常散射法确定SUVH9的结构，并精修至的分辨率，R因子为18.7％，并且R free为22.3％(图8B)。SUVH9的结构包含三个区段：朝向N-末端的双-螺旋束(残基138-194)、SRA结构域(残基195-379)和pre-SET/SET结构域(残基380-637)(图8B)。
SUVH9结构的独特特征是朝向N-末端的双-螺旋束的形成和定位，所述双-螺旋束夹在SRA结构域和pre-SET/SET结构域之间，从而与它们二者相互作用。两个α-螺旋之间的界面富含疏水性残基，所述疏水性残基参与广泛的范德华相互作用，补充有在较短的第二螺旋的Arg178和Lys182之间插入较长的第一螺旋的Glu166后的盐桥形成(图8C)。这些疏水性相互作用和盐桥使两个螺旋的相对位置稳定，并充当用于将SRA结构域和pre-SET/SET结构域锚定在双-螺旋束的任一侧的支架，从而导致形成整个蛋白的总体牢固的拓扑结构。
双-螺旋束深埋在SRA结构域和pre-SET/SET结构域的界面处，其相对排列通过形成广泛的疏水性和亲水性相互作用而被稳定。双-螺旋束的较长的第一螺旋(残基138-169)插入SRA和pre-SET/SET结构域之间的界面。较长的螺旋的N-末端区段非常疏水，并与周围残基形成若干对盐桥和氢键(图8D)。相比之下，该螺旋的C-末端部分包含大的且连续疏水性的表面，所述表面与较短的第二螺旋(图8C)和蛋白的其他结构域(图8E)相互作用。一个长环从SET结构域中延伸出来(图8E)，并覆盖较长的螺旋的外表面。该环的尖端包含若干疏水性残基(Leu559、Val562和Leu563)，所述疏水性残基与较长的螺旋的一个面上的疏水性残基(Ile154、Leu158、Phe161和Leu162)形成广泛的范德华相互作用。不希望囿于理论，认为较长的螺旋区段可能在SUVH9蛋白的总体架构的稳定化中起重要的作用。较短的第二螺旋主要与较长的螺旋的C-末端部分相互作用(图8B、8C)，辅以与SRA结构域的少许氢键和疏水性相互作用(图8F)。
SUVH9的SRA结构域类似于报道的来自UHRF1和SUVH5的SRA结构域的结构(Arita等人,2008；Avvakumov等人,2008；Hashimoto等人,2008；Rajakumara等人,2011)。SUVH9和SUVH5 SRA结构域的叠合产生145个排列的α碳原子的的RMSD，与两个蛋白几乎相同的折叠一致，所述两个蛋白的核心元件包括六条链的扭曲的β-桶和两个α-螺旋。与在SUVH5SRA结构域中观察到的无序环不同，SUVH9 SRA结构域的C末端形成α-螺旋，随后是短转角，且然后直接连接至pre-SET结构域。
通过SRA与pre-SET/SET结构域之间的广泛直接的相互作用以及通过与双-螺旋束的相互作用，使SRA与pre-SET/SET结构域之间的相对朝向稳定。SRA结构域与pre-SET/SET结构域之间的直接相互作用主要由疏水性相互作用占主导(图8G)。具体地，SRA结构域的C-末端延伸的α-螺旋使用疏水性表面以与pre-SET/SET结构域的连续疏水性表面相互作用，SRA结构域的α-螺旋侧翼的残基也有助于疏水性界面的形成。SUVH9的pre-SET/SET结构域的结构类似于发表的H3K9组蛋白甲基转移酶例如Dim5、G9a和GLP的结构(Wu等人,2010；Zhang等人,2002；Zhang等人,2003)。SUVH9的pre-SET/SET结构域与人类H3K9甲基转移酶GLP的叠合产生252个排列的α碳原子的的RMSD，虽然它们仅共有34％的序列同一性。如在其他SET结构域蛋白中通常观察到的，SUVH9的pre-SET结构域的9个保守的Cys残基与三个Zn2+离子配位以形成等边三角形簇(图8B)。
虽然SUVH9包含组蛋白甲基转移酶样pre-SET和SET结构域，它既未表现出可检测的组蛋白甲基转移酶活性，也未表现出对SAM辅因子的体外结合能力(Johnson等人,2008)。在一级序列中，虽然SUVH9保留有助于甲基转移酶活性的对应于人类GLP的Tyr1124和Tyr1211的两个Tyr残基(Tyr522和Tyr636)(Wu等人,2010；Zhang等人,2002；Zhang等人,2003)，SUVH9缺乏对于辅因子和肽底物结合以及催化重要的post-SET结构域。人类GLP和SUVH9的pre-SET和SET结构域采取相似的折叠拓扑结构(Wu等人,2010)。在人类GLP中，SAH结合口袋相对窄，并且表现出显著的对SAH的结构和化学互补性(图9A)。此外，具有覆盖SAH的腺嘌呤部分能力的post-SET结构域的存在发挥作用以使该辅因子分子的结合稳定。相比之下，SUVH9的推测的SAH结合口袋开口很大(图9B)，并且不能保持结合的SAH分子，特别是在不存在post-SET结构域的稳定作用的情况下。另外，通常SUV家族活性甲基转移酶利用来自SET结构域的一个Cys残基以及来自post-SET结构域的三个Cys残基以与Zn2+离子配位，以使post-SET结构域的富含环的区域的相对位置和构象稳定。相比之下，在SUVH9的SET结构域中，Cys残基被Thr替换，使得即使伴侣蛋白带来三个Cys残基，它仍然不可能形成Zn2+稳定的配位中心。
在包含SET结构域的H3K9甲基转移酶中，肽底物位于SET和post-SET结构域之间(图9C)，并通过与酶形成广泛的主链和侧链分子间相互作用而被稳定。在人类H3K9甲基转移酶GLP中，SET结构域的酸性环区(残基1145-1153)参与识别肽底物(图9C)。相比之下，SUVH9中的对应区段包含富含疏水性残基的25个残基的插入环(图8H)。因为插入环通过与来自SET和pre-SET结构域的残基形成广泛的疏水性相互作用而被稳定(图8H)，特别是环的尖端，其覆盖双-螺旋束并与双-螺旋束广泛地相互作用(图8H)，插入环采取良好界定的拓扑结构。SUVH9结构的检查鉴定了在插入环和推测结合的肽底物之间具有空间位阻的可能性，从而阻止肽底物进入SUVH9蛋白的肽结合裂缝中(图9D)。
该实施例证实了SUVH2和SUVH9是将Pol V募集至包含DNA甲基化的区域所需要的。此外，使用ZF-SUVH2融合物表明，SUVH2靶向作用足以将Pol V和DNA甲基化募集至未甲基化的靶基因。不希望囿于理论，认为SUVH2和SUVH9的作用机制提供了之前存在的DNA甲基化和随后一轮的RAN指导的DNA甲基化之间的加强环路。
实施例3
以下实施例涉及修饰的拟南芥SHH1蛋白的产生和表征，所述SHH1蛋白被功能化以包含将所修饰的SHH1蛋白靶向至特定核酸序列的核酸结合结构域。在该实施例中，SHH1靶向至APETALA1基因座的启动子区域与伴随siRNA在APETALA1处的富集。
材料和方法
TAL-SHH1的构建
以前(Law等人,2011)描述了包含SHH1的Gateway入门克隆(entry clone)，所述克隆包括1.4kb的启动子区域以及具有C末端的BLRP和3xMyc标签的ORF。通过定点诱变(Quickchange II,Agilent)使用引物SHH1deltaBsaI_fo，5’-GGGAGAGTGAACGTTGGTGACCTGCTTCTATGTTT-3’(SEQ ID NO:142)、SHH1deltaBsaI_re和5’AAACATAGAAGCAGGTCACCAACGTTCACTCTCCC3’(SEQ ID NO:143)从SHH1入门克隆中去除BsaI识别位点。去除BsaI位点之后，通过用XhoI限制性消化而线性化入门克隆。为了将编码修饰的基于Hax3的转录激活因子样效应子(TALE)(Sanjana等人,2012)的DNA序列符合读框地插入到SHH1的C末端BLRP和3xMyc标签之间，其中所述转录激活因子样效应子排除了C末端核定位信号和酸性激活结构域、侧翼为GSSGSS接头，使用引物TALETFintotag_fo,5’-CCAAGGACCTCTCGAGGGATCTTCAGGTTCATCTTCGCGGACCCGGCTCCCT-3’(SEQ ID NO:144)和TALETFintoSHH1Myc_re,5’-CATAGATCCCTCGAGTGAAGAACCAGATGATCCGCTAGCTGACGCGCGA-3’(SEQ ID NO:145)从TALE转录激活因子(TALE-TF)质粒(Addgene)扩增包括ccdB选择盒的2.8kb的TALE骨架。使用In-Fusion HD克隆系统(Clontech)将扩增的产物与线性化的入门克隆融合。通过Golden Gate克隆从18个单体-重复序列(Addgene)组装TALE DNA结合结构域，所述TALE DNA结合结构域靶向对应于APETALA1(AT1G69120)的启动子区域中1号染色体核苷酸位置25986363至25986381的序列5’-TTAGGATTTGCGTGTCGAC-3’(SEQ ID NO:146)，并将所述TALE DNA结合结构域插到ccdB选择盒侧翼的BsaI位点之间(Sanjana等人,2012)。列出了编码TALE靶重复序列的DNA序列(SEQ ID NO:140)。为了促进TAL-SHH1融合蛋白与甲基化的胞嘧啶结合,使用重复可变的“双残基”(RVD)N*(Valton等人,2012)。通过分别使用引物NN>N*_fo,5’-GTGGCAATTGCGAGCAACGGGGGAAAGCAG-3’(SEQ ID NO:147)，NN>N*_re,5’-CTGCTTTCCCCCGTTGCTCGCAATTGCCAC-3’(SEQ ID NO:148),NN>NH_fo,5’-GGCAATTGCGAGCAACCATGGGGGAAAGCAGGCAC-3’(SEQ ID NO:149)和NN>NH_re,5’-GTGCCTGCTTTCCCCCATGGTTGCTCGCAATTGCC-3’(SEQ ID NO:150)，对TALE单体模板质粒pNN_v2(Addgene)定点诱变，产生编码单体RVD N*和NH的质粒。通过使用引物TALETF_NN>N*_fo,5’-TGGCTATTGCATCCAACGGGGGCAGACC-3’(SEQ ID NO:151)和TALETF_NN>N*_re,5’-GGTCTGCCCCCGTTGGATGCAATAGCCA-3’(SEQ ID NO:152)对pTALE-TF_v2(NN)(Addgene)定点诱变，产生编码RVD N*的TALE-TF质粒。使用LR clonase II(Life Technologies)和包含hph选择标记的修饰的pEarlyGate302目标载体产生双元载体(Law等人,2011)。
植物材料
使用蘸花法通过根癌农杆菌菌株ABL0将TAL-SHH1构建体转化到生态型Col-0的shh1(SALK_074540)中(Clough和Bent,1998)。分别用潮霉素B选择T1植株，并通过蛋白质分离和使用针对3xMyc的抗体的蛋白质印迹测试嵌合TAL-SHH1蛋白的表达。选择表现出强的转基因表达的T1植株。
结果
选择包含被导向APETALA1基因的TAL-SHH1的植株之后，产生小RNA文库，并使用已确定的方案(Law等人,2013)对所述文库进行深度测序，以检测响应于将SHH1靶向启动子而在APETALA1基因处产生的小RNA的存在。作为对照，对来自野生型植株的小RNA进行测序，预测其不会包含存在于APETALA1的启动子区域中的siRNA。如图18所示，检测到了对应于TAL-SHH1植株而非野生型植株中的DNA的两条链的小RNA，表明修饰的SHH1蛋白能够将小RNA靶向至AP1启动子。
实施例4
以下实施例强调了经由SUVH2/SUVH9的DNA甲基化与Pol V结合之间的关系，并证实了SUVH2与DRD1相互作用。
材料和方法
拟南芥中的实验方案
除了在以下缓冲液中使磨碎的组织与甲醛交联之外，按照Bernatavichute等人(2008)中的步骤，进行染色质免疫沉淀：10mM HEPES pH 8.0、1M蔗糖、5mM KCl、5mM MgCl2、5mM EDTA，和0.6％Triton X-100。使用的所有引物列于以下表12中。使用OvationUltralow DR Multiplex System(NuGen)产生用于NRPE1 ChIP-Seq的文库。如在Johnson等人(2008)中进行的,使用EZ DNA Methylation-Gold试剂盒(Zymo Research)进行亚硫酸氢盐测序，随后进行PCR扩增和FWA片段的克隆。按照实施例1中的描述产生BS-Seq文库，并使用HiSeq 2000平台遵循制造商的说明(Illumina)以50bp的长度对所有文库进行测序。按照实施例2所述产生锌指构建体。
表12：引物列表
数据分析
使用BS-seeker将亚硫酸氢盐-Seq(BS-Seq)读段比对至TAIR 10版本的拟南芥参考基因组。对于BS-Seq，允许最高达2个错配，并仅使用独特映射的读段。
免疫共沉淀实验
用pLJ322(JP7468中的ZF-HA-SUVH2)浸润本氏烟草(Nicotiana benthamiana)，三天后收集叶片。将未浸润的叶片用作阴性对照。在液氮中磨碎3g组织，并将其重悬于12ml的包含1μg/ml抑肽素、1mM PMSF和1X完全蛋白酶抑制剂tab-EDTA(Roche)的IP缓冲液(50mMTris pH 8.0、150mM NaCl、5mM MgCl2、10％甘油和0.1％NP40)中。将提取物过滤通过神奇滤布(miracloth)，以3,000g离心5min。添加200μl HA-磁珠(MRL)，并在4℃下旋转45min。用IP缓冲液洗涤3次后，然后将这些与以相同方式制备的DRD1-Flag拟南芥花提取物一起孵育。在4℃下旋转孵育持续45min。用IP缓冲液洗涤珠子3次，并在60μl SDS染料中煮沸。用抗-Flag-HRP或抗-HA-HRP抗体探测蛋白质印迹。进行拟南芥的免疫共沉淀(co-IP)实验，所述实验以来自表达HA-SUVH2、Flag-DRD1的植株，或包含HA-SUVH2和Flag-DRD1二者的T2植株的2g花起始。如上所述制备提取物，添加100μl的Flag-磁珠(Sigma)，在4℃下旋转孵育45min。如上所述进行洗涤和蛋白质印迹。
结果
实施例2中呈现的结果阐明了与DNA结合结构域基序融合的SUVH蛋白如何能够被靶向至未甲基化的靶基因，以及将Pol V和DNA甲基化募集至未甲基化的靶基因。图11中示出了多种SUVH蛋白的序列比对。实施例2还确立了SUVH2和SUVH9不仅是Pol V活性所需要的，而且是它与整个基因组染色质稳定缔合所需要的。
为了确定SUVH2和SUVH9对限定的Pol V结合位点处的DNA甲基化的影响，如实施例2所见利用全基因组亚硫酸氢盐测序(BS-Seq)。还分析了单突变体suvh2和suvh9的BS-Seq结果，以确定SUVH2/SUVH9是否在所有位点都冗余地作用，或者是否在它们发挥作用时具有非重叠位点。发现在Pol V位点以及在suvh2或suvh9单突变体或者在suvh2 suvh9双突变体中限定的差异性甲基化区域(DMR)，suvh2具有比suvh9更强的作用(图12)。这些结果表明，SUVH2和SUVH9在整个基因组冗余地作用，以控制RNA指导的DNA甲基化。
不希望囿于理论，实施例2呈现的结果还表明，经由SUVH2/SUVH9的DNA甲基化和Pol V结合之间存在加强的环路。为了进一步研究这一理论，利用保持性甲基转移酶MET1的突变，所述突变消除基因组范围内的CG甲基化，还减少CHG和CHH甲基化(Aufsatz等人,2004；Stroud等人,2013；Lister等人,2008)(图13b)。使用内源性NRPE1抗体，ChIP-seq表明，与野生型的通常被Pol V占据的位点相比，Pol V在met1中的占据基本上被消除(图13c)。相反，在met1中以前被鉴定为获得甲基化的一系列位点(Stroud等人,2013)，观察到Pol V结合的增加(图13d和图13e)。这些结果表明，DNA甲基化是Pol V募集的一个重要组成部分。示出SUVH2和SUVH9二者的SRA结构域中的点突变引起RNA指导的DNA甲基化的丧失，表明SRA结构域有助于发挥作用(Johnson等人,2008)。总之，并且不希望囿于理论，这些结果表明，SUVH2/SUVH9与甲基化的DNA的结合募集Pol V，从而在之前存在的DNA甲基化和随后通过RdDM通路的甲基化募集之间提供联系。
还发现，与观察到的ZF-SUVH2/fwa-4植株系的结果不同，在fwa-4背景中与靶向FWA的锌指融合的KYP蛋白(ZF-KYP/fwa-4)以与fwa-4突变体类似的时间开花(与野生型相比开花较晚)。包括ZF-KYP作为另外的阴性对照，因为KYP(也被称为SUVH4)是RdDM不需要的SUVH蛋白。还通过ChIP表明在FWA处存在对照ZF-KYP(图15A)。
为了进一步研究该靶向，使用如图6所述的来自实施例2的植株系，使用亚硫酸氢盐测序以确定FWA基因沉默是否与DNA甲基化相关。在野生型株系中，检测到很大区域的DNA甲基化，具有特别高水平的CG甲基化(图14A)。在fwa-4和包含ZF-KYP或HA-SUVH2的转化体中，该区域均完全没有DNA甲基化(参见ZF-KYP，图14A)。在三个独立的被分析的在fwa-4中包含ZF-SUVH2的T1株系中，在所有胞嘧啶序列背景中紧靠Zn指位点的位置观察到高水平的DNA甲基化，所述DNA甲基化随着下游被转录区域而逐渐减少(实施例2，图6E)。将所述株系中的一个传代(ZF-SUVH2-2)至T2和T3代，并使用BS-seq以确定甲基化延伸的范围(图14A)。发现甲基化从结合位点在任一方向延伸了大约150个碱基对，并且不会在代之间显著扩张。还观察到，在已经失去ZF-SUVH2转基因的T2分离株中保留了该DNA甲基化和沉默(图15B)。这些结果表明，将SUVH2靶向至FWA足以诱导DNA甲基化和基因沉默，并且该表观遗传沉默状态可以在不存在ZF-SUVH2的情况下被保留。
从实施例2中，实验被设计以测试ZF-SUVH2是否募集Pol V。使用NRPE1 ChIP以检查野生型、nrpe1和wa-4株系中FWA处的Pol V水平。在野生型和fwa-4株系中观察到在两个已知的Pol V位点IGN5和IGN22处的Pol V的富集，但是在nrpe1突变体株系中则未观察到(图7A)。在野生型中观察到在FWA的启动子和转录区域二者处的Pol V的富集，但是在nrpe1或fwa-4中则未观察到(图7A)。因此，Pol V存在于野生型的处于其DNA甲基化和沉默状态的FWA处，但是不存在于其fwa-4表观等位基因的未甲基化状态中。为了确定ZF-SUVH2与FWA的结合是否募集Pol V，在fwa-4中包含ZF-SUVH2的T2株系中进行NRPE1 ChIP。与亲本fwa-4株系不同，ZF-SUVH2株系表现出在FWA处的Pol V富集(图7A)。总之，这些结果表明，SUVH2足以将Pol V定位在FWA启动子处，导致DNA甲基化和基因沉默。
为了得到SUVH2或SUVH9靶向RdDM的可能机制，查询通过利用多种表位-加标记的RdDM的免疫沉淀蛋白复合物产生的质谱数据集。虽然在来自Pol V纯化的一些IP-质谱研究(利用NRPE1-Flag)中尚未观察到SUVH2/9肽，但是在两个独立的DRD1质谱数据集中鉴定了SUVH2肽(图16)。DRD1是DDR复合物(还包含DMS3和RDM1)的组分，所述DDR复合物与Pol V相互作用(Law等人,2010)，并且是在整个基因组范围内Pol V占据所需要的。(Zhong等人，2012)。观察到的SUVH2肽的数量比来自是DDR复合物的化学计量组分DMS3和RDM1蛋白的那些更低，并且也比多数Pol V复合物组分的肽的水平更低，表明SUVH2与DRD1的相互作用比DDR组分之间或DDR与Pol V之间的相互作用更弱或者更加短暂。为了证实DRD1与SUVH2之间的相互作用，在本氏烟草的叶子中表达表位加标签的SUVH2，并且在HA磁珠上对其纯化。使用此作为SUVH2的来源，特异性地且可重复地从转基因拟南芥蛋白质提取物中拉下DRD1-Flag(图14B：珠子-SUVH2)。作为阴性对照，使用不表达加标签的SUVH2的本氏烟草进行相同测定，并且在该测定中不能检测到DRD1-Flag(图14B：磁珠-假试验)。另外，使用转基因拟南芥植株中的HA加标签的SUVH2和Flag加标签的DRD1进行免疫沉淀实验，并且也观察到这些蛋白质之间的相互作用(图15C)。这些结果证实，不希望囿于理论，IP-质谱观察结果与以下模型一致：SUVH2经由与包括DRD1在内的DDR复合物组分短暂相互作用而间接发挥作用以募集Pol V。
实施例5
该实施例证实了RNA指导的DNA甲基化途径的不同组分的靶向将Pol V募集至特定基因座。
简介
RNA指导的DNA甲基化(RdDM)途径介导植物中的从头开始的DNA甲基化。实施例2和4证实了ZF-SUVH2用于特异性靶向靶基因座的甲基化和沉默的用途。这通过利用FWA基因作为靶而实现。FWA的表达引起拟南芥中强的晚开花表型。野生型植株中存在的该基因的启动子处的甲基化，引起FWA的转录沉默，并导致相对于fwa-4突变体的早开花表型。fwa-4拟南芥表观遗传突变体表现出在FWA基因的启动子中无甲基化，并因此表现出典型的晚开花表型(相对于野生型)。实施例2和4描述了与锌指(ZF)蛋白ZF108融合的嵌合SUVH2蛋白，所述锌指蛋白被设计为靶向拟南芥中FWA的启动子，并且证实了该融合蛋白能够促进fwa-4植株中该基因组位点处的甲基化。该甲基化靶向伴随着Pol V募集被至该(FWA)位点，并且导致产生引起甲基化、基因沉默所需要的非编码RNA，并因此产生早开花表型。
材料和方法
融合蛋白构建
为了产生该实例中描述的不同融合蛋白，用限制性酶XhoI消化实施例2中描述的pUC57质粒中的ZF108片段，并将其直接插入不同基因中的独特XhoI位点中，或者插入到包含Flag-BLRP或HA-BLRP融合物的pCR2质粒中，所述Flag-BLRP或HA-BLRP融合物包含位于标签和BLRP之间的XhoI位点并且侧翼为AscI限制性位点。如下所述构建各个构建体。
DRD1-HA-ZF：用AscI消化pCR2质粒中的HA-ZF108-BLRP融合物，并将其插入pENTR-DRD1质粒的单个AscI位点中(Law等人,2010)，所述AscI位点位于DRD1编码序列末端之后6个碱基对处。
DMS3-Flag-ZF：用AscI消化pCR2质粒中的Flag-ZF108-BLRP融合物，并将其插入pENTR-DMS3质粒的单个AscI位点中(Law等人,2010)，所述AscI位点位于DMS3编码序列末端之后6个碱基对处。
RDM1-Flag-ZF：为了这个目的，产生质粒pENTR-RDM1，其包含RDM1的基因组序列，所述FRG的基因组序列包括350个碱基对的5'启动子序列。然后用AscI消化pCR2质粒中的Flag-ZF108-BLRP融合物，并将其插入pENTR-RDM1质粒的单个AscI位点中，所述单个AscI位点位于RDM1编码序列末端之后6个碱基对处。
RDM1-HA-ZF：为了这个目的，产生质粒pENTR-RDM1，其包含RDM1的基因组序列，所述RDM1的基因组序列包括350个碱基对的5'启动子序列。然后用AscI消化pCR2质粒中的HA-ZF108-BLRP融合物，并将其插入pENTR-RDM1质粒的单个AscI位点中，所述单个AscI位点位于RDM1编码序列末端之后6个碱基对处。
HA-ZF-DRM3：为了这个目的，使用质粒pENTR-BLRP-HA-DRM3，其包含DRM3的基因组序列，所述DRM3的基因组序列包括2500个碱基对的5'启动子序列和在起始密码子前的BLRP-HA融合物。然后用XhoI消化pUC57质粒中的ZF108片段，并将其插入pENTR-BLRP-HA-DRM3质粒的单个XhoI位点中，所述单个XhoI位点位于BLRP和HA标签之间。
HA-2XZF-DRM3：为了这个目的，使用质粒pENTR-BLRP-HA-DRM3，其包含DRM3的基因组序列，所述DRM3的基因组序列包括2500个碱基对的5'启动子序列和在起始密码子前的BLRP-HA融合物。然后用XhoI消化pUC57质粒中的ZF108片段，并将两个拷贝的ZF108串联插入pENTR-BLRP-HA-DRM3质粒的单个XhoI位点中，所述单个XhoI位点位于BLRP和HA标签之间。
ZF-3F9M-DRM2：用EcoRI消化pUC57中侧翼为EcoRI位点的ZF108片段，并将其插入质粒pENTR-3F9M-DRM2(Henderson等人,2010)中的单个EcoRI位点中，该EcoRI位点位于3xFlag-9xMyc标签的5'起始端。
FRG-Flag-ZF：为了这个目的，使用质粒pENTR-FRG，其包含FRG的基因组序列，所述基因组序列包括800个碱基对的5'启动子序列。用AscI消化pCR2质粒中的Flag-ZF108-BLRP融合物，并将其插入pENTR-FRG质粒的单个AscI位点中，所述AscI位点位于FRG编码序列末端之后6个碱基对处。
SUVR2-Flag-ZF：使用质粒pENTR-SUVR2，其包含SUVR2的基因组序列，所述基因组序列包括2000个碱基对的5'启动子序列。用AscI消化pCR2质粒中的Flag-ZF108-BLRP融合物，并将其插入pENTR-SUVR2质粒的单个AscI位点中，所述AscI位点位于SUVR2编码序列末端之后6个碱基对处。
SUVR2-HA-ZF：使用质粒pENTR-SUVR2，其包含SUVR2的基因组序列，所述基因组序列包括2000个碱基对的5'启动子序列。用AscI消化pCR2质粒中的HA-ZF108-BLRP融合物，并将其插入pENTR-SUVR2质粒的单个AscI位点中，所述AscI位点位于SUVR2编码序列末端之后6个碱基对处。
MORC6-HA-ZF：使用质粒pENTR-MORC6，其包含MORC6的基因组序列，所述基因组序列包括2463个碱基对的5'启动子序列。用AscI消化pCR2质粒中的HA-ZF108-BLRP融合物，并将其插入pENTR-MORC6质粒的单个AscI位点中，所述AscI位点位于MORC6编码序列末端之后6个碱基对处。
Flag-ZF-CMT2：产生质粒pENTR-Flag-CMT2，其包含CMT2的基因组序列，所述基因组序列包括1032个碱基对的5'启动子序列和在起始密码子前的BLRP-Flag融合物。用XhoI消化pUC57质粒中的ZF108片段，并将其插入pENTR-BLRP-Flag-CMT2质粒的单个XhoI位点中，所述XhoI位点位于BLRP和Flag标签之间。
将融合蛋白引入fwa-4
将上文描述的所有ZN-108融合蛋白构建体重组到载体JP726中，并使用农杆菌介导的转化将其引入fwa-4。用BASTA选择转化的株系。
结果
为了探测多种其它的RdDM蛋白是否具有在fwa-4突变体中的FWA基因座处触发从头开始的DNA甲基化的能力，进行了一系列实验试图将RdDM通路的不同组分靶向至拟南芥中FWA基因的启动子。选择参与RdDM的多种蛋白，并将所述蛋白融合至靶向FWA启动子的ZF108锌指(参见实施例2)，并转化到fwa-4突变体中。如上文所述以与实施例2和4中所述的那些类似的方法构建ZF-靶向株系。对～30个独立的转基因株系的开花时间评分。下表13中示出了选择的不同RdDM组分和开花时间结果的列表。
表13：与fwa-4相比T1株系中的早开花
表13中所示的结果证实了DMS3-ZF和MORC6-ZF可在fwa-4突变体背景中有效地促进早开花。SUVR2-Flag-ZF靶向在30个植株中产生了3个早开花植株，但是这些植株的开花不如由DMS3或MORC6靶向的植株早。并且，SUVR2-HA-ZF未产生早开花植株。SUVR2结果表明，当被融合至靶向FWA的DNA结合结构域时，SUVR2具有至少部分靶向和沉默FWA的能力。重要的是，在这些第一代T1植株中，DDR复合物中的三个蛋白质(DRD1、DMS3和RDM1)中，只有DMS3在有效地引起相对于fwa-4的早开花中有效。并且，DRM2的直接靶向无效。因此，结果表明，至少在第一代T1植株中，并非所有的RdDM组分都在有效地靶向DNA甲基化中有效。
还从表13可见，MORC6-HA-ZF在fwa-4突变体中引起早开花的方面有效。MORC1(At4g36290)和MORC2(AT4G36280)是与MORC6相关的蛋白，并且申请人已经表明它们与MORC6形成稳定的异源二聚体。申请人之前表明MORC6和MORC1参与基因沉默(Moissiard等人,2012)。申请人还表明，MORC1 MORC2双突变体具有与MORC6突变体非常相似的表型。因此，认为MORC1和MORC1b非常可能也成功地靶向Pol V、DNA甲基化和基因沉默。
为了分析表13中所述的早开花株系的早开花表型是否归因于FWA启动子的甲基化，在表现出相对于fwa-4的早开花表型的两个独立的DMS3-ZF株系和3个独立的MORC6-ZF株系中进行全基因组亚硫酸氢盐测序测定。以与实施例4中描述的方法类似的方法进行亚硫酸氢盐测序实验。结果表明，以与之前实施例4中所示用于SUVH2的方法非常类似的方式在FWA处再确立了DNA甲基化(图17)。因此，DMS3和MORC6有效地将甲基化靶向FWA启动子。如上文所述，ZF-SUVR2株系在引起fwa-4突变体中早开花中稍微有效，从而也可能将DNA甲基化靶向至FWA。
为了进一步分析这些转基因植物中FWA沉默的稳定性，以及测试这些融合蛋白是否可能仅在较后代表现出影响，允许每个株系的大量T1植株自花传粉，并测定T2代的开花时间，如表14所示。衍生自早开花的来自DMS3-ZF转化株的T1植株的T2植株T2世代中表现出100％的早开花植株，证实了FWA基因沉默的有效性和稳定性。衍生自早开花的来自MORC6-ZF转化株的T1植株的T2植株表现出一些变化，但是仍有大多数在T2代中表现出100％的早开花植株。衍生自早开花的来自SUVR2-ZF转化株的T1植株的T2植株表现出更多变化，并且只有一个在T2代中表现出100％的早开花植株。
表14：T2株系中早开花
对于其它融合蛋白的T2代的分析表明，甚至对于在T1代中未表现出影响的一些RdDM因子，在T2代中可见到部分影响。这可见于DRD1-ZF、RDM1-ZF、DRM3-ZF、DRM2-ZF和FRG-ZF(FRG是申请人已经表明参与RdDM的基因At3g20010)融合物构建体。这证实了DRD1、RDM1、DRM3、DRM2和FRG基因也可以用于靶向Pol V、DNA甲基化和基因沉默，虽然这些基因的有效性可能比较低，并且它可能需要多个植物世代才能观察到影响。作为对照，包括不参与RdDM的CMT2蛋白，并且观察到所有T2植株表现出100％晚开花，表明CMT2在靶向FWA沉默中不是有效的。
申请人已经表明，不同的RdDM蛋白可以被靶向以便以不同程度的沉默功效沉默特异性的基因座。具有可以提供一系列不同的基因沉默功效的一组蛋白质可能会有优势。例如，取决于待沉默的靶核酸，选择具有不同沉默功效的本公开内容的重组蛋白可以是有利的。例如，通过选择非常有效的RdDM组分以将尽可能多的甲基化定向至靶基因而完全沉默所述靶基因的表达，可能是有利的。在其它情况下，靶向功效较低的RdDM组分以将较少的甲基化靶向至基因以引起仅部分沉默效应，可能是有利的。当基因以不同水平表达时，它们通常对植物表型表现出不同影响，并且可能植物基因的部分沉默的情况是最有利的。
实施例6
以下实施例涉及修饰的拟南芥SHH1蛋白的产生和表征，所述修饰的拟南芥SHH1蛋白被工程化为包含将修饰的SHH1蛋白靶向至特定的核酸序列的核酸结合结构域，还涉及修饰的拟南芥SUVH2蛋白的产生和表征，所述修饰的拟南芥SUVH2蛋白被工程化为包含将修饰的SUVH2蛋白靶向至特定的核酸序列的核酸结合结构域。在该实施例中，SHH1和SUVH2同时靶向至SUPERMAN启动子区域与SUPERMAN处的DNA甲基化富集相关。
材料和方法
TAL-SHH1和TAL-SUVH2嵌合体的构建
以前(Johnson等人,2008)描述了包含SUVH2的Gateway入门克隆，所述克隆包括1.4kb的启动子区域以及具有N末端的3xHA和BLRP标签的ORF。以前(Law等人,2011)描述了包含SHH1的Gateway入门克隆，所述克隆包括1.4kb的启动子区域以及具有C末端的BLRP和3xMyc标签的ORF。通过定点诱变(Quickchange multi,Agilent)使用引物SUVH2deltaBsaI1，5’-TCGTTGTCTCGCCGAAATTCGAAAGACCGAGAGAG-3’(SEQ ID NO:168)、SUVH2deltaBsaI2，5’-TCATCATAGTGTTGTTATATCTTTGGTGTCTCTGTCTTGCTTC-3’(SEQ ID NO:175)和SUVH2deltaBsaI3，5’-CCTTTAATTTCCTTTTTTGTTTGCGTCTCTACTCTCTACAATCTATA-3’(SEQ ID NO:169)从SHH1入门克隆中去除BsaI识别位点。通过定点诱变(Quickchange II,Agilent)使用引物SHH1deltaBsaI_fo，5’-GGGAGAGTGAACGTTGGTGACCTGCTTCTATGTTT-3’(SEQ ID NO:170)、SHH1deltaBsaI_re，5’AAACATAGAAGCAGGTCACCAACGTTCACTCTCCC3’(SEQID NO:171)从SHH1入门克隆中去除BsaI识别位点。去除BsaI位点之后，通过用XhoI限制性消化而线性化入门克隆。为了将编码修饰的基于Hax3的转录激活因子样效应子(TALE)(Sanjana等人,2012)的DNA序列符合读框地插入到SHH1的C末端BLRP和3xMyc标签之间，其中所述转录激活因子样效应子排除了C末端核定位信号和酸性激活结构域、侧翼为GSSGSS接头，使用引物TALETFintotag_fo，5’-CCAAGGACCTCTCGAGGGATCTTCAGGTTCATCTTCGCGGACCCGGCTCCCT-3’(SEQ ID NO:144)和分别TALETFintoSHH1Myc_re，5’-CATAGATCCCTCGAGTGAAGAACCAGATGATCCGCTAGCTGACGCGCGA-3’(SEQ ID NO:145)或TALETFintoSUVH2tag_re，5’-GGTATCCCATCTCGAGTGAAGAACCAGATGATCCGCTAGCTGACGCGCGA-3’(SEQ ID NO:172)从TALE转录激活因子(TALE-TF)质粒(Addgene)扩增包括ccdB选择盒的2.8kb的TALE骨架。使用In-Fusion HD克隆系统(Clontech)将扩增子与线性化的入门克隆融合。通过Golden Gate克隆从18个单体-重复序列(Addgene)组装两个TALE DNA结合结构域，所述TALE DNA结合结构域靶向对应于SUPERMAN(AT3G23130)的启动子区域中3号染色体的核苷酸位置8242204至8242222的序列5’-GGGGATTTGATAATGCGTC-3’(SEQ ID NO:173)和3号染色体的核苷酸位置8242251至8242233的序列5’-GTTAAGACTGTGAAAGAGA-3’(SEQ ID NO:174)，并将所述两个TALE DNA结合结构域插到ccdB选择盒侧翼的BsaI位点之间(Sanjana等人,2012)。列出了编码TALE靶重复序列的DNA序列(SEQ ID NO:140)，所述TALE靶重复序列对应于SUP_D(SEQ IDNO:138)和SUP_P(SEQ ID NO:139)。为了促进TALE融合蛋白与甲基化的胞嘧啶结合，使用重复可变的“双残基”(RVD)N*(Valton等人,2012)。通过分别使用引物NN>N*_fo,5’-GTGGCAATTGCGAGCAACGGGGGAAAGCAG-3’(SEQ ID NO:147)、NN>N*_re,5’-CTGCTTTCCCCCGTTGCTCGCAATTGCCAC-3’(SEQ ID NO:148)、NN>NH_fo,5’-GGCAATTGCGAGCAACCATGGGGGAAAGCAGGCAC-3’(SEQ ID NO:149)和NN>NH_re，5’-GTGCCTGCTTTCCCCCATGGTTGCTCGCAATTGCC-3’(SEQ ID NO:150)，对TALE单体模板质粒pNN_v2(Addgene)定点诱变，产生编码单体RVD N*和NH的质粒。通过使用引物TALETF_NN>N*_fo,5’-TGGCTATTGCATCCAACGGGGGCAGACC-3’(SEQ ID NO:151)和TALETF_NN>N*_re,5’-GGTCTGCCCCCGTTGGATGCAATAGCCA-3’(SEQ ID NO:152)，对pTALE-TF_v2(NN)(Addgene)定点诱变，产生编码RVD N*的TALE-TF质粒。使用LR克隆酶II(Life Technologies)和包含Bar或hph选择标记的修饰的pEarlyGate302目标载体产生双元载体(Law等人,2011)。
植物材料
使用蘸花法通过根癌农杆菌菌株ABL0将TAL-SHH1构建体转化到生态型Col-0的shh1(SALK_074540)中，并将TAL-SUVH2转化到生态型Col-0的suvh2(SALK_079574)中(Clough和Bent,1998)。用草铵磷或潮霉素B选择T1植株，并通过蛋白质分离和分别使用抗3xMyc或3xHA标签的抗体的蛋白质印迹，测试嵌合TAL-SHH1或TAL-SUVH2蛋白的表达。将表现出强的转基因表达的T1植株杂交，目的是结合TAL-SUVH2和TAL-SHH1或者用于SUPERMAN(At3g23130)基因靶区域的发夹构建体。通过PCR测定F1植株的基因型，以确定所述转基因的存在。
结果
选择包含被导向SUPERMAN基因的TAL-SHH1、被导向SUPERMAN基因的TAL-SUVH2任一种的F1植株，或包含TAL-SHH1和TAL-SUVH2转基因二者的植株之后，使用已确定的方案(Law等人,2013)制备全基因组亚硫酸氢盐文库。对这些文库进行深度测序，如之前所述将读段映射到拟南芥基因组上(Law等人,2013)。如图19所示，在包含TAL-SHH1和TAL-SUVH2转基因二者的植株中在SUPERMAN基因处检测到CHHDNA甲基化，但是在单独包含TAL-SHH1或者TAL-SUVH2转基因的植株中则未检测到。这些结果表明，将SHH1和SUVH2靶向至SUPERMAN启动子区域足以引起CHH DNA甲基化。
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实施方案的陈述
1.一种用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：
(a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含SHH1多肽或其片段；以及
(b)在由所述重组核酸编码的所述重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。
2.实施方案1的方法，其中所述DNA结合结构域包含锌指结构域。
3.实施方案2的方法，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
4.实施方案2的方法，其中所述锌指结构域为锌指阵列。
5.实施方案2的方法，其中所述锌指结构域选自由以下组成的组：Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
6.实施方案1的方法，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域(ribbon-helix-helix domain)、TBP结构域、桶状二聚体结构域(barrel dimer domain)、真实同源结构域(real homology domain)、BAH结构域、SANT结构域、克罗莫结构域(Chromodomain)、Tudor结构域、布罗莫结构域(Bromodomain)、PHD结构域、WD40结构域和MBD结构域。
7.实施方案1的方法，其中所述DNA结合结构域包含TAL效应子靶向结构域。
8.实施方案1的方法，其中所述DNA结合结构域包含3个C2H2锌指结构域。
9.实施方案1-8任一项的方法，其中所述第二氨基酸序列包含同源结构域或SAWADEE结构域中的至少一个。
10.实施方案1的方法，其中所述第二氨基酸序列包含与SEQ ID NO:1至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
11.实施方案1的方法，其中所述第二氨基酸序列包含与SEQ ID NO:1 100％相同的氨基酸序列。
12.实施方案1-11任一项的方法，其中所述重组多肽与RNA聚合酶相互作用。
13.实施方案12的方法，其中所述RNA聚合酶为RNA聚合酶IV。
14.实施方案1-13任一项的方法，其中所述重组多肽引起RNA指导的DNA甲基化。
15.实施方案1-14任一项的方法，其中所述一种或更多种靶核酸为内源核酸。
16.实施方案1-14任一项的方法，其中所述一种或更多种靶核酸为异源核酸。
17.实施方案1-16任一项的方法，其中所述一种或更多种靶核酸的表达被沉默。
18.一种重组核酸，所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的SHH1样蛋白，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含SHH1多肽或其片段。
19.实施方案18的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:1至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
20.实施方案18的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:1 100％相同的氨基酸序列。
21.实施方案18-20任一项的重组核酸，其中所述DNA结合结构域包含锌指结构域。
22.实施方案21的重组核酸，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
23.实施方案21的重组核酸，其中所述锌指结构域为锌指阵列。
24.实施方案21的重组核酸，其中所述锌指结构域选自由以下组成的组：C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
25.实施方案18-20任一项的重组核酸，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
26.实施方案18-20任一项的重组核酸，其中所述DNA结合结构域包含TAL效应子靶向结构域。
27.实施方案18-20任一项的重组核酸，其中所述DNA结合结构域结合一种或更多种靶核酸。
28.实施方案27的重组核酸，其中所述一种或更多种靶核酸为编码多肽的核酸。
29.实施方案27的重组核酸，其中所述一种或更多种靶核酸为内源植物核酸。
30.实施方案27的重组核酸，其中所述一种或更多种靶核酸为异源核酸。
31.实施方案27-30任一项的重组核酸，其中所述SHH1样蛋白减少所述一种或更多种靶核酸的表达。
32.实施方案27-30任一项的重组核酸，其中所述SHH1样蛋白使所述一种或更多种靶核酸的表达沉默。
33.一种载体，所述载体包含实施方案18-32任一项的重组核酸，其中所述重组核酸被可操作地连接至调节序列。
34.一种宿主细胞，所述宿主细胞包含实施方案33的表达载体。
35.实施方案34的宿主细胞，其中所述宿主细胞为植物细胞。
36.一种重组植物，所述重组植物包含实施方案18-32任一项的重组核酸。
37.一种用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：
(a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含SUVH2多肽或其片段、或SUVH9多肽或其片段；以及
(b)在由所述重组核酸编码的所述重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。
38.实施方案37的方法，其中所述DNA结合结构域包含锌指结构域。
39.实施方案38的方法，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
40.实施方案38的方法，其中所述锌指结构域为锌指阵列。
41.实施方案38的方法，其中所述锌指结构域选自由以下组成的组：Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
42.实施方案37的方法，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
43.实施方案37的方法，其中所述DNA结合结构域包含TAL效应子靶向结构域。
44.实施方案37的方法，其中所述DNA结合结构域包含3个C2H2锌指结构域。
45.实施方案37-45任一项的方法，其中所述第二氨基酸序列包含选自由以下组成的组的结构域：双螺旋束结构域、SRA结构域、pre-SET结构域和SET结构域。
46.实施方案37的方法，其中所述第二氨基酸序列包含与SEQ ID NO:14或SEQ IDNO:27至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
47.实施方案37的方法，其中所述第二氨基酸序列包含与SEQ ID NO:14或SEQ IDNO:27 100％相同的氨基酸序列。
48.实施方案37-47任一项的方法，其中所述重组多肽与RNA聚合酶相互作用。
49.实施方案48的方法，其中所述RNA聚合酶为RNA聚合酶V。
50.实施方案37-49任一项的方法，其中所述重组多肽引起RNA指导的DNA甲基化。
51.实施方案37-50任一项的方法，其中所述一种或更多种靶核酸为内源核酸。
52.实施方案37-50任一项的方法，其中所述一种或更多种靶核酸为异源核酸。
53.实施方案37-52任一项的方法，其中所述一种或更多种靶核酸的表达被沉默。
54.一种重组核酸，所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的SUVH2样蛋白或SUVH9样蛋白，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含SUVH2多肽或其片段、或SUVH9多肽或其片段。
55.实施方案54的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:14或SEQID NO:27至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
56.实施方案54的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:14或SEQID NO:27 100％相同的氨基酸序列。
57.实施方案54-56任一项的重组核酸，其中所述DNA结合结构域包含锌指结构域。
58.实施方案57的重组核酸，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
59.实施方案57的重组核酸，其中所述锌指结构域为锌指阵列。
60.实施方案57的重组核酸，其中所述锌指结构域选自由以下组成的组：C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
61.实施方案54-56任一项的重组核酸，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
62.实施方案54-56任一项的重组核酸，其中所述DNA结合结构域包含TAL效应子靶向结构域。
63.实施方案54-62任一项的重组核酸，其中所述DNA结合结构域结合一种或更多种靶核酸。
64.实施方案63的重组核酸，其中所述一种或更多种靶核酸为编码多肽的核酸。
65.实施方案63的重组核酸，其中所述一种或更多种靶核酸为内源植物核酸。
66.实施方案63的重组核酸，其中所述一种或更多种靶核酸为异源核酸。
67.实施方案63-66任一项的重组核酸，其中所述SUVH2样蛋白或SUVH9样蛋白减少所述一种或更多种靶核酸的表达。
68.一种载体，所述载体包含实施方案54-67任一项的重组核酸，其中所述重组核酸被可操作地连接至调节序列。
69.一种宿主细胞，所述宿主细胞包含实施方案68的表达载体。
70.实施方案69的宿主细胞，其中所述宿主细胞为植物细胞。
71.一种重组植物，所述重组植物包含实施方案54-67任一项的重组核酸。
72.一种用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：
(a)提供植物，所述植物包含：
第一重组核酸，所述第一重组核酸编码包含第一氨基酸序列和第二氨基酸序列的第一重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含SHH1多肽或其片段，和
第二重组核酸，所述第二重组核酸编码包含第一氨基酸序列和第二氨基酸序列的第二重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含SUVH2多肽或其片段、或SUVH9多肽或其片段；以及
(b)在由所述第一重组核酸编码的所述第一重组多肽和由所述第二重组核酸编码的所述第二重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。
73.实施方案72的方法，其中所述第一重组多肽或第二重组多肽的至少一个引起RNA指导的DNA甲基化。
74.实施方案72-73任一项的方法，其中所述一种或更多种靶核酸为内源核酸。
75.实施方案72-73任一项的方法，其中所述一种或更多种靶核酸为异源核酸。
76.实施方案72-75任一项的方法，其中所述一种或更多种靶核酸的表达被沉默。
77.一种宿主细胞，所述宿主细胞包含实施方案33和68的表达载体。
78.实施方案77的宿主细胞，其中所述宿主细胞为植物细胞。
79.一种重组植物，所述重组植物包含实施方案18-32任一项和实施方案54-67任一项的重组核酸。
80.一种用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：
(a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含DMS3多肽或其片段；以及
(b)在由所述重组核酸编码的所述重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。
81.实施方案80的方法，其中所述DNA结合结构域包含锌指结构域。
82.实施方案81的方法，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
83.实施方案81的方法，其中所述锌指结构域为锌指阵列。
84.实施方案81的方法，其中所述锌指结构域选自由以下组成的组：Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
85.实施方案80的方法，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
86.实施方案80的方法，其中所述DNA结合结构域包含TAL效应子靶向结构域。
87.实施方案80的方法，其中所述DNA结合结构域包含3个C2H2锌指结构域。
88.实施方案80的方法，其中所述第二氨基酸序列包含与SEQ ID NO:41至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
89.实施方案80的方法，其中所述第二氨基酸序列包含与SEQ ID NO:41 100％相同的氨基酸序列。
90.实施方案80-89任一项的方法，其中所述重组多肽与RNA聚合酶相互作用。
91.实施方案80-90任一项的方法，其中所述重组多肽引起RNA指导的DNA甲基化。
92.实施方案80-91任一项的方法，其中所述一种或更多种靶核酸为内源核酸。
93.实施方案80-91任一项的方法，其中所述一种或更多种靶核酸为异源核酸。
94.实施方案80-93任一项的方法，其中所述一种或更多种靶核酸的表达被沉默。
95.一种重组核酸，所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的DMS3样蛋白，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含DMS3多肽或其片段。
96.实施方案95的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:41至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
97.实施方案96的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:41100％相同的氨基酸序列。
98.实施方案95-97任一项的重组核酸，其中所述DNA结合结构域包含锌指结构域。
99.实施方案98的重组核酸，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
100.实施方案98的重组核酸，其中所述锌指结构域为锌指阵列。
101.实施方案98的重组核酸，其中所述锌指结构域选自由以下组成的组：C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
102.实施方案95-97任一项的重组核酸，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
103.实施方案95-97任一项的重组核酸，其中所述DNA结合结构域包含TAL效应子靶向结构域。
104.实施方案95-97任一项的重组核酸，其中所述DNA结合结构域结合一种或更多种靶核酸。
105.实施方案104的重组核酸，其中所述一种或更多种靶核酸为编码多肽的核酸。
106.实施方案104的重组核酸，其中所述一种或更多种靶核酸为内源植物核酸。
107.实施方案104的重组核酸，其中所述一种或更多种靶核酸为异源核酸。
108.实施方案104-107任一项的重组核酸，其中所述DMS3样蛋白减少所述一种或更多种靶核酸的表达。
109.实施方案104-107任一项的重组核酸，其中所述DMS3样蛋白使所述一种或更多种靶核酸的表达沉默。
110.一种载体，所述载体包含实施方案95-109任一项的重组核酸，其中所述重组核酸被可操作地连接至调节序列。
111.一种宿主细胞，所述宿主细胞包含实施方案110的表达载体。
112.实施方案111的宿主细胞，其中所述宿主细胞为植物细胞。
113.一种重组植物，所述重组植物包含实施方案95-112任一项的重组核酸。
114.一种用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：
(a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含MORC6多肽或其片段；以及
(b)在由所述重组核酸编码的所述重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。
115.实施方案114的方法，其中所述DNA结合结构域包含锌指结构域。
116.实施方案115的方法，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
117.实施方案115的方法，其中所述锌指结构域为锌指阵列。
118.实施方案115的方法，其中所述锌指结构域选自由以下组成的组：Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
119.实施方案114的方法，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
120.实施方案114的方法，其中所述DNA结合结构域包含TAL效应子靶向结构域。
121.实施方案114的方法，其中所述DNA结合结构域包含3个C2H2锌指结构域。
122.实施方案114的方法，其中所述第二氨基酸序列包含与SEQ ID NO:53至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
123.实施方案114的方法，其中所述第二氨基酸序列包括与SEQ ID NO:53 100％相同的氨基酸序列。
124.实施方案114-123任一项的方法，其中所述重组多肽与RNA聚合酶相互作用。
125.实施方案114-123任一项的方法，其中所述重组多肽引起RNA指导的DNA甲基化。
126.实施方案114-125任一项的方法，其中所述一种或更多种靶核酸为内源核酸。
127.实施方案114-125任一项的方法，其中所述一种或更多种靶核酸为异源核酸。
128.实施方案114-127任一项的方法，其中所述一种或更多种靶核酸的表达被沉默。
129.一种重组核酸，所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的MORC6样蛋白，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含MORC6多肽或其片段。
130.实施方案129的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:53至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
131.实施方案130的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:53100％相同的氨基酸序列。
132.实施方案129-131任一项的重组核酸，其中所述DNA结合结构域包含锌指结构域。
133.实施方案132的重组核酸，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
134.实施方案132的重组核酸，其中所述锌指结构域为锌指阵列。
135.实施方案132的重组核酸，其中所述锌指结构域选自由以下组成的组：C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
136.实施方案129-131任一项的重组核酸，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
137.实施方案129-131任一项的重组核酸，其中所述DNA结合结构域包含TAL效应子靶向结构域。
138.实施方案129-131任一项的重组核酸，其中所述DNA结合结构域结合一种或更多种靶核酸。
139.实施方案138的重组核酸，其中所述一种或更多种靶核酸为编码多肽的核酸。
140.实施方案138的重组核酸，其中所述一种或更多种靶核酸为内源植物核酸。
141.实施方案138的重组核酸，其中所述一种或更多种靶核酸为异源核酸。
142.实施方案138-141任一项的重组核酸，其中所述MORC6样蛋白减少所述一种或更多种靶核酸的表达。
143.实施方案138-141任一项的重组核酸，其中所述MORC6样蛋白使所述一种或更多种靶核酸的表达沉默。
144.一种载体，所述载体包含实施方案114-143任一项的重组核酸，其中所述重组核酸被可操作地连接至调节序列。
145.一种宿主细胞，所述宿主细胞包含实施方案144的表达载体。
146.实施方案145的宿主细胞，其中所述宿主细胞为植物细胞。
147.一种重组植物，所述重组植物包含实施方案114-143任一项的重组核酸。
148.一种用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：
(a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含SUVR2多肽或其片段；以及
(b)在由所述重组核酸编码的所述重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。
149.实施方案148的方法，其中所述DNA结合结构域包含锌指结构域。
150.实施方案149的方法，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
151.实施方案149的方法，其中所述锌指结构域为锌指阵列。
152.实施方案149的方法，其中所述锌指结构域选自由以下组成的组：Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
153.实施方案148的方法，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
154.实施方案148的方法，其中所述DNA结合结构域包含TAL效应子靶向结构域。
155.实施方案148的方法，其中所述DNA结合结构域包含3个C2H2锌指结构域。
156.实施方案148的方法，其中所述第二氨基酸序列包含与SEQ ID NO:66至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
157.实施方案148的方法，其中所述第二氨基酸序列包含与SEQ ID NO:66 100％相同的氨基酸序列。
158.实施方案148-157任一项的方法，其中所述重组多肽与RNA聚合酶相互作用。
159.实施方案148-157任一项的方法，其中所述重组多肽诱导RNA指导的DNA甲基化。
160.实施方案148-159任一项的方法，其中所述一种或更多种靶核酸为内源核酸。
161.实施方案148-159任一项的方法，其中所述一种或更多种靶核酸为异源核酸。
162.实施方案148-161任一项的方法，其中所述一种或更多种靶核酸的表达被沉默。
163.一种重组核酸，所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的SUVR2样蛋白，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含SUVR2多肽或其片段。
164.实施方案163的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:66至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
165.实施方案164的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:66100％相同的氨基酸序列。
166.实施方案163-165任一项的重组核酸，其中所述DNA结合结构域包含锌指结构域。
167.实施方案166的重组核酸，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
168.实施方案166的重组核酸，其中所述锌指结构域为锌指阵列。
169.实施方案166的重组核酸，其中所述锌指结构域选自由以下组成的组：C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
170.实施方案163-165任一项的重组核酸，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
171.实施方案163-165任一项的重组核酸，其中所述DNA结合结构域包含TAL效应子靶向结构域。
172.实施方案163-165任一项的重组核酸，其中所述DNA结合结构域结合一种或更多种靶核酸。
173.实施方案172的重组核酸，其中所述一种或更多种靶核酸为编码多肽的核酸。
174.实施方案172的重组核酸，其中所述一种或更多种靶核酸为内源植物核酸。
175.实施方案172的重组核酸，其中所述一种或更多种靶核酸为异源核酸。
176.实施方案172-175任一项的重组核酸，其中所述SUVR2样蛋白减少所述一种或更多种靶核酸的表达。
177.实施方案172-175任一项的重组核酸，其中所述SUVR2样蛋白使所述一种或更多种靶核酸的表达沉默。
178.一种载体，所述载体包含实施方案163-177任一项的重组核酸，其中所述重组核酸被可操作地连接至调节序列。
179.一种宿主细胞，所述宿主细胞包含实施方案178的表达载体。
180.实施方案179的宿主细胞，其中所述宿主细胞为植物细胞。
181.一种重组植物，所述重组植物包含实施方案163-177任一项的重组核酸。
182.一种用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：
(a)提供植物，所述植物包含：
第一重组核酸，所述第一重组核酸编码包含第一氨基酸序列和第二氨基酸序列的第一重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含SHH1多肽或其片段，和
一种或更多种另外的重组核酸，所述一种或更多种另外的重组核酸编码一种或更多种另外的多肽，所述一种或更多种另外的多肽的每种包含第一氨基酸序列和第二氨基酸序列，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含选自由以下组成的组的多肽：SUVH2多肽或其片段、SUVH9多肽或其片段、DMS3多肽或其片段、MORC6多肽或其片段和SUVR2多肽或其片段；以及
(b)在由所述第一重组核酸编码的所述第一重组多肽和由所述一种或更多种另外的重组核酸编码的所述一种或更多种另外的多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。
183.实施方案182的方法，其中所述重组多肽的至少一个引起RNA指导的DNA甲基化。
184.实施方案182-183任一项的方法，其中所述一种或更多种靶核酸为内源核酸。
185.实施方案182-183任一项的方法，其中所述一种或更多种靶核酸为异源核酸。
186.实施方案182-185任一项的方法，其中所述一种或更多种靶核酸的表达被沉默。
187.一种宿主细胞，所述宿主细胞包含表达载体，所述表达载体包含编码实施方案182的重组多肽的重组核酸。
188.实施方案187的宿主细胞，其中所述宿主细胞为植物细胞。
189.一种重组植物，所述重组植物包含实施方案182的重组核酸。
190.一种用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：
(a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含DRD1多肽或其片段；以及
(b)在由所述重组核酸编码的所述重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。
191.实施方案190的方法，其中所述DNA结合结构域包含锌指结构域。
192.实施方案191的方法，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
193.实施方案191的方法，其中所述锌指结构域为锌指阵列。
194.实施方案191的方法，其中所述锌指结构域选自由以下组成的组：Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
195.实施方案190的方法，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
196.实施方案190的方法，其中所述DNA结合结构域包含TAL效应子靶向结构域。
197.实施方案190的方法，其中所述DNA结合结构域包含3个C2H2锌指结构域。
198.实施方案190的方法，其中所述第二氨基酸序列包含与SEQ ID NO:79至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
199.实施方案100的方法，其中所述第二氨基酸序列包含与SEQ ID NO:79 100％相同的氨基酸序列。
200.实施方案190-199任一项的方法，其中所述重组多肽与RNA聚合酶相互作用。
201.实施方案190-199任一项的方法，其中所述重组多肽引起RNA指导的DNA甲基化。
202.实施方案190-201任一项的方法，其中所述一种或更多种靶核酸为内源核酸。
203.实施方案190-201任一项的方法，其中所述一种或更多种靶核酸为异源核酸。
204.实施方案190-203任一项的方法，其中所述一种或更多种靶核酸的表达被沉默。
205.一种重组核酸，所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的DRD1样蛋白，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含DRD1多肽或其片段。
206.实施方案205的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:79至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
207.实施方案206的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:79100％相同的氨基酸序列。
208.实施方案205-207任一项的重组核酸，其中所述DNA结合结构域包含锌指结构域。
209.实施方案208的重组核酸，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
210.实施方案208的重组核酸，其中所述锌指结构域为锌指阵列。
211.实施方案208的重组核酸，其中所述锌指结构域选自由以下组成的组：C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
212.实施方案205-207任一项的重组核酸，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
213.实施方案205-207任一项的重组核酸，其中所述DNA结合结构域包含TAL效应子靶向结构域。
214.实施方案205-207任一项的重组核酸，其中所述DNA结合结构域结合一种或更多种靶核酸。
215.实施方案214的重组核酸，其中所述一种或更多种靶核酸为编码多肽的核酸。
216.实施方案214的重组核酸，其中所述一种或更多种靶核酸为内源植物核酸。
217.实施方案214的重组核酸，其中所述一种或更多种靶核酸为异源核酸。
218.实施方案214-217任一项的重组核酸，其中所述DRD1样蛋白减少所述一种或更多种靶核酸的表达。
219.实施方案214-217任一项的重组核酸，其中所述DRD1样蛋白使所述一种或更多种靶核酸的表达沉默。
220.一种载体，所述载体包含实施方案205-219任一项的重组核酸，其中所述重组核酸被可操作地连接至调节序列。
221.一种宿主细胞，所述宿主细胞包含实施方案220的表达载体。
222.实施方案221的宿主细胞，其中所述宿主细胞为植物细胞。
223.一种重组植物，所述重组植物包含实施方案205-219任一项的重组核酸。
224.一种用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：
(a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含RDM1多肽或其片段；以及
(b)在由所述重组核酸编码的所述重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。
225.实施方案224的方法，其中所述DNA结合结构域包含锌指结构域。
226.实施方案225的方法，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
227.实施方案225的方法，其中所述锌指结构域为锌指阵列。
228.实施方案225的方法，其中所述锌指结构域选自由以下组成的组：Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
229.实施方案224的方法，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
230.实施方案224的方法，其中所述DNA结合结构域包含TAL效应子靶向结构域。
231.实施方案224的方法，其中所述DNA结合结构域包含3个C2H2锌指结构域。
232.实施方案224的方法，其中所述第二氨基酸序列包含与SEQ ID NO:91至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
233.实施方案232的方法，其中所述第二氨基酸序列包含与SEQ ID NO:91 100％相同的氨基酸序列。
234.实施方案224-233任一项的方法，其中所述重组多肽与RNA聚合酶相互作用。
235.实施方案224-233任一项的方法，其中所述重组多肽引起RNA指导的DNA甲基化。
236.实施方案224-235任一项的方法，其中所述一种或更多种靶核酸为内源核酸。
237.实施方案224-235任一项的方法，其中所述一种或更多种靶核酸为异源核酸。
238.实施方案224-237任一项的方法，其中所述一种或更多种靶核酸的表达被沉默。
239.一种重组核酸，所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的RDM1样蛋白，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含RDM1多肽或其片段。
240.实施方案239的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:91至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
241.实施方案240的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:91100％相同的氨基酸序列。
242.实施方案239-241任一项的重组核酸，其中所述DNA结合结构域包含锌指结构域。
243.实施方案242的重组核酸，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
244.实施方案242的重组核酸，其中所述锌指结构域为锌指阵列。
245.实施方案242的重组核酸，其中所述锌指结构域选自由以下组成的组：C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
246.实施方案239-241任一项的重组核酸，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
247.实施方案239-241任一项的重组核酸，其中所述DNA结合结构域包含TAL效应子靶向结构域。
248.实施方案239-241任一项的重组核酸，其中所述DNA结合结构域结合一种或更多种靶核酸。
249.实施方案248的重组核酸，其中所述一种或更多种靶核酸为编码多肽的核酸。
250.实施方案248的重组核酸，其中所述一种或更多种靶核酸为内源植物核酸。
251.实施方案248的重组核酸，其中所述一种或更多种靶核酸为异源核酸。
252.实施方案248-251任一项的重组核酸，其中所述RDM1样蛋白减少所述一种或更多种靶核酸的表达。
253.实施方案248-251任一项的重组核酸，其中所述RDM1样蛋白使所述一种或更多种靶核酸的表达沉默。
254.一种载体，所述载体包含实施方案239-253任一项的重组核酸，其中所述重组核酸被可操作地连接至调节序列。
255.一种宿主细胞，所述宿主细胞包含实施方案254的表达载体。
256.实施方案255的宿主细胞，其中所述宿主细胞为植物细胞。
257.一种重组植物，所述重组植物包含实施方案239-253任一项的重组核酸。
258.一种用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：
(a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含DRM3多肽或其片段；以及
(b)在由所述重组核酸编码的所述重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。
259.实施方案258的方法，其中所述DNA结合结构域包含锌指结构域。
260.实施方案259的方法，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
261.实施方案259的方法，其中所述锌指结构域为锌指阵列。
262.实施方案259的方法，其中所述锌指结构域选自由以下组成的组：Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
263.实施方案258的方法，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
264.实施方案258的方法，其中所述DNA结合结构域包含TAL效应子靶向结构域。
265.实施方案258的方法，其中所述DNA结合结构域包含3个C2H2锌指结构域。
266.实施方案258的方法，其中所述第二氨基酸序列包含与SEQ ID NO:101至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
267.实施方案266的方法，其中所述第二氨基酸序列包含与SEQ ID NO:101 100％相同的氨基酸序列。
268.实施方案258-267任一项的方法，其中所述重组多肽与RNA聚合酶相互作用。
269.实施方案258-267任一项的方法，其中所述重组多肽引起RNA指导的DNA甲基化。
270.实施方案258-269任一项的方法，其中所述一种或更多种靶核酸为内源核酸。
271.实施方案258-269任一项的方法，其中所述一种或更多种靶核酸为异源核酸。
272.实施方案258-271任一项的方法，其中所述一种或更多种靶核酸的表达被沉默。
273.一种重组核酸，所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的DRM3样蛋白，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含DRM3多肽或其片段。
274.实施方案273的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:101至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
275.实施方案274的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:101100％相同的氨基酸序列。
276.实施方案273-275任一项的重组核酸，其中所述DNA结合结构域包含锌指结构域。
277.实施方案276的重组核酸，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
278.实施方案276的重组核酸，其中所述锌指结构域为锌指阵列。
279.实施方案276的重组核酸，其中所述锌指结构域选自由以下组成的组：C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
280.实施方案273-275任一项的重组核酸，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
281.实施方案273-275任一项的重组核酸，其中所述DNA结合结构域包含TAL效应子靶向结构域。
282.实施方案273-275任一项的重组核酸，其中所述DNA结合结构域结合一种或更多种靶核酸。
283.实施方案282的重组核酸，其中所述一种或更多种靶核酸为编码多肽的核酸。
284.实施方案282的重组核酸，其中所述一种或更多种靶核酸为内源植物核酸。
285.实施方案282的重组核酸，其中所述一种或更多种靶核酸为异源核酸。
286.实施方案282-285任一项的重组核酸，其中所述DRM3样蛋白减少所述一种或更多种靶核酸的表达。
287.实施方案282-285任一项的重组核酸，其中所述DRM3样蛋白使所述一种或更多种靶核酸的表达沉默。
288.一种载体，所述载体包含实施方案273-287任一项的重组核酸，其中所述重组核酸被可操作地连接至调节序列。
289.一种宿主细胞，所述宿主细胞包含实施方案288的表达载体。
290.实施方案289的宿主细胞，其中所述宿主细胞为植物细胞。
291.一种重组植物，所述重组植物包含实施方案273-287任一项的重组核酸。
292.一种用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：
(a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含DRM2多肽或其片段；以及
(b)在由所述重组核酸编码的所述重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。
293.实施方案292的方法，其中所述DNA结合结构域包含锌指结构域。
294.实施方案293的方法，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
295.实施方案293的方法，其中所述锌指结构域为锌指阵列。
296.实施方案293的方法，其中所述锌指结构域选自由以下组成的组：Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
297.实施方案292的方法，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
298.实施方案292的方法，其中所述DNA结合结构域包含TAL效应子靶向结构域。
299.实施方案292的方法，其中所述DNA结合结构域包含3个C2H2锌指结构域。
300.实施方案292的方法，其中所述第二氨基酸序列包含与SEQ ID NO:113至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
301.实施方案300的方法，其中所述第二氨基酸序列包含与SEQ ID NO:113 100％相同的氨基酸序列。
302.实施方案292-301任一项的方法，其中所述重组多肽与RNA聚合酶相互作用。
303.实施方案292-301任一项的方法，其中所述重组多肽引起RNA指导的DNA甲基化。
304.实施方案292-303任一项的方法，其中所述一种或更多种靶核酸为内源核酸。
305.实施方案292-303任一项的方法，其中所述一种或更多种靶核酸为异源核酸。
306.实施方案292-305任一项的方法，其中所述一种或更多种靶核酸的表达被沉默。
307.一种重组核酸，所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的DRM2样蛋白，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含DRM2多肽或其片段。
308.实施方案307的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:113至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
309.实施方案308的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:113100％相同的氨基酸序列。
310.实施方案307-309任一项的重组核酸，其中所述DNA结合结构域包含锌指结构域。
311.实施方案310的重组核酸，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
312.实施方案310的重组核酸，其中所述锌指结构域为锌指阵列。
313.实施方案310的重组核酸，其中所述锌指结构域选自由以下组成的组：C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
314.实施方案307-309任一项的重组核酸，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
315.实施方案307-309任一项的重组核酸，其中所述DNA结合结构域包含TAL效应子靶向结构域。
316.实施方案307-309任一项的重组核酸，其中所述DNA结合结构域结合一种或更多种靶核酸。
317.实施方案316的重组核酸，其中所述一种或更多种靶核酸为编码多肽的核酸。
318.实施方案316的重组核酸，其中所述一种或更多种靶核酸为内源植物核酸。
319.实施方案316的重组核酸，其中所述一种或更多种靶核酸为异源核酸。
320.实施方案316-319任一项的重组核酸，其中所述DRM2样蛋白减少所述一种或更多种靶核酸的表达。
321.实施方案316-319任一项的重组核酸，其中所述DRM2样蛋白使所述一种或更多种靶核酸的表达沉默。
322.一种载体，所述载体包含实施方案307-321任一项的重组核酸，其中所述重组核酸被可操作地连接至调节序列。
323.一种宿主细胞，所述宿主细胞包含实施方案322的表达载体。
324.实施方案323的宿主细胞，其中所述宿主细胞为植物细胞。
325.一种重组植物，所述重组植物包含实施方案307-321任一项的重组核酸。
326.一种用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：
(a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，第二氨基酸序列包含FRG多肽或其片段；以及
(b)在由所述重组核酸编码的所述重组多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。
327.实施方案326的方法，其中所述DNA结合结构域包含锌指结构域。
328.实施方案327的方法，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
329.实施方案327的方法，其中所述锌指结构域为锌指阵列。
330.实施方案327的方法，其中所述锌指结构域选自由以下组成的组：Cys2His2(C2H2)锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
331.实施方案326的方法，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
332.实施方案326的方法，其中所述DNA结合结构域包含TAL效应子靶向结构域。
333.实施方案326的方法，其中所述DNA结合结构域包含3个C2H2锌指结构域。
334.实施方案326的方法，其中所述第二氨基酸序列包含与SEQ ID NO:125至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
335.实施方案334的方法，其中所述第二氨基酸序列包含与SEQ ID NO:125 100％相同的氨基酸序列。
336.实施方案326-335任一项的方法，其中所述重组多肽与RNA聚合酶相互作用。
337.实施方案326-335任一项的方法，其中所述重组多肽引起RNA指导的DNA甲基化。
338.实施方案326-337任一项的方法，其中所述一种或更多种靶核酸为内源核酸。
339.实施方案326-337任一项的方法，其中所述一种或更多种靶核酸为异源核酸。
340.实施方案326-339任一项的方法，其中所述一种或更多种靶核酸的表达被沉默。
341.一种重组核酸，所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的FRG样蛋白，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含FRG多肽或其片段。
342.实施方案341的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:125至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
343.实施方案342的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:125100％相同的氨基酸序列。
344.实施方案341-343任一项的重组核酸，其中所述DNA结合结构域包含锌指结构域。
345.实施方案344的重组核酸，其中所述锌指结构域包含2个、3个、4个、5个、6个、7个、8个或9个锌指。
346.实施方案344的重组核酸，其中所述锌指结构域为锌指阵列。
347.实施方案344的重组核酸，其中所述锌指结构域选自由以下组成的组：C2H2锌指结构域、CCCH锌指结构域、多半胱氨酸锌指结构域和锌双核簇结构域。
348.实施方案341-343任一项的重组核酸，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
349.实施方案341-343任一项的重组核酸，其中所述DNA结合结构域包含TAL效应子靶向结构域。
350.实施方案341-343任一项的重组核酸，其中所述DNA结合结构域结合一种或更多种靶核酸。
351.实施方案350的重组核酸，其中所述一种或更多种靶核酸为编码多肽的核酸。
352.实施方案350的重组核酸，其中所述一种或更多种靶核酸为内源植物核酸。
353.实施方案350的重组核酸，其中所述一种或更多种靶核酸为异源核酸。
354.实施方案350-353任一项的重组核酸，其中所述FRG样蛋白减少所述一种或更多种靶核酸的表达。
355.实施方案350-353任一项的重组核酸，其中所述FRG样蛋白使所述一种或更多种靶核酸的表达沉默。
356.一种载体，所述载体包含实施方案341-355任一项的重组核酸，其中所述重组核酸被可操作地连接至调节序列。
357.一种宿主细胞，所述宿主细胞包含实施方案356的表达载体。
358.实施方案357的宿主细胞，其中所述宿主细胞为植物细胞。
359.一种重组植物，所述重组植物包含实施方案341-355任一项的重组核酸。
360.一种用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：
(a)提供植物，所述植物包含：
第一重组核酸，所述第一重组核酸编码包含第一氨基酸序列和第二氨基酸序列的第一重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含SHH1多肽或其片段，和
一种或更多种另外的重组核酸，所述一种或更多种另外的重组核酸编码一种或更多种另外的多肽，所述一种或更多种另外的多肽的每种包含第一氨基酸序列和第二氨基酸序列，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含选自由以下组成的组的多肽：SUVH2多肽或其片段、SUVH9多肽或其片段、DMS3多肽或其片段、MORC6多肽或其片段、SUVR2多肽或其片段、DRD1多肽或其片段、RDM1多肽或其片段、DRM3多肽或其片段、DRM2多肽或其片段和FRG多肽或其片段，以及
(b)在由所述第一重组核酸编码的所述第一重组多肽和由所述一种或更多种另外的重组核酸编码的所述一种或更多种另外的多肽藉以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。
361.实施方案360的方法，其中所述重组多肽的至少一种引起RNA指导的DNA甲基化。
362.实施方案360-361任一项的方法，其中所述一种或更多种靶核酸为内源核酸。
363.实施方案360-361任一项的方法，其中所述一种或更多种靶核酸为异源核酸。
364.实施方案360-363任一项的方法，其中所述一种或更多种靶核酸的表达被沉默。
365.一种宿主细胞，所述宿主细胞包含编码实施方案360的重组多肽的重组核酸的表达载体。
366.实施方案365的宿主细胞，其中所述宿主细胞为植物细胞。
367.一种重组植物，所述重组植物包含实施方案360的重组核酸。
368.一种植物，所述植物由于实施方案1-17、37-53、80-94、114-128、148-162、190-204、224-238、258-272、292-306和326-340任一项的方法而具有一种或更多种靶核酸的减少的表达。
369.实施方案368的植物的后代植物。
370.实施方案369的后代植物，其中所述后代植物具有所述一种或更多种靶核酸的减少的表达且不包含所述重组核酸。
371.一种植物，所述植物由于实施方案72-76任一项的方法而具有一种或更多种靶核酸的减少的表达。
372.实施方案371的植物的后代植物。
373.实施方案372的后代植物，其中所述后代植物具有所述一种或更多种靶核酸的减少的表达且不包含所述第一重组核酸或第二重组核酸。
374.一种植物，所述植物由于实施方案182-186和360-364任一项的方法而具有一种或更多种靶核酸的减少的表达。
375.实施方案374的植物的后代植物。
376.实施方案375的后代植物，其中所述后代植物具有所述一种或更多种靶核酸的减少的表达且不包含所述第一重组核酸或所述一种或更多种另外的重组核酸。
序列表
<110> 史蒂夫·E·雅各布森
朱莉·劳
丽安娜·约翰逊
<120> 用于将RNA聚合酶和非编码RNA生物发生靶向特定基因座的方法和组合物
<130> 26223-2000440
<140> 还未指定
<141> 同时附上
<150> US 61/771,743
<151> 2013-03-01
<150> US 61/929,414
<151> 2014-01-20
<160> 175
<170> FastSEQ for Windows Version 4.0
<210> 1
<211> 258
<212> PRT
<213> 拟南芥(Arabidopsis thaliana)
<400> 1
Met Ala Ala Ser Asp Asp Ser Ser His Tyr Phe Thr Glu Phe Thr Leu
1 5 10 15
Ser Glu Ile Val Asp Met Glu Asn Leu Tyr Lys Glu Leu Gly Asp Gln
20 25 30
Ser Leu His Lys Asp Phe Cys Gln Thr Val Ala Ser Thr Phe Ser Cys
35 40 45
Ser Val Asn Arg Asn Gly Lys Ser Ser Ile Thr Trp Lys Gln Val Gln
50 55 60
Ile Trp Phe Gln Glu Lys Leu Lys His Gln Ser Gln Pro Lys Ser Lys
65 70 75 80
Thr Leu Pro Ser Pro Pro Leu Gln Ile His Asp Leu Ser Asn Pro Ser
85 90 95
Ser Tyr Ala Ser Asn Ala Ser Asn Ala Thr Phe Val Gly Asn Ser Thr
100 105 110
Phe Val Gln Thr Arg Lys Gly Lys Ala Ser Asp Leu Ala Asp Leu Ala
115 120 125
Phe Glu Ala Lys Ser Ala Arg Asp Tyr Ala Trp Tyr Asp Val Ser Ser
130 135 140
Phe Leu Thr Tyr Arg Val Leu Arg Thr Gly Glu Leu Glu Val Arg Val
145 150 155 160
Arg Phe Ser Gly Phe Asp Asn Arg His Asp Glu Trp Val Asn Val Lys
165 170 175
Thr Ser Val Arg Glu Arg Ser Ile Pro Val Glu Pro Ser Glu Cys Gly
180 185 190
Arg Val Asn Val Gly Asp Leu Leu Leu Cys Phe Gln Glu Arg Glu Asp
195 200 205
Gln Ala Leu Tyr Cys Asp Gly His Val Leu Asn Ile Lys Arg Gly Ile
210 215 220
His Asp His Ala Arg Cys Asn Cys Val Phe Leu Val Arg Tyr Glu Leu
225 230 235 240
Asp Asn Thr Glu Glu Ser Leu Gly Leu Glu Arg Ile Cys Arg Arg Pro
245 250 255
Glu Glu
<210> 2
<211> 285
<212> PRT
<213> 蓖麻(Ricinus communis)
<400> 2
Met Thr Ala Ser His Glu Leu Gly Gly His Arg Arg Pro His Thr Arg
1 5 10 15
Glu Thr Leu Glu Thr Ser Leu Thr Thr Thr Gln Asn Pro Val Asn Ile
20 25 30
Ala Lys Gln Lys Ala Gln Ser Leu Phe Trp Leu Val Met Gly Ser Pro
35 40 45
Ser Glu Phe Thr Leu Ala Glu Met Val Glu Met Glu Asn Ile Tyr Lys
50 55 60
Glu Leu Gly Glu Glu Ser Leu Asp Ser Glu Phe Cys Glu Arg Leu Ala
65 70 75 80
Thr Ser Phe Ser Phe Thr Ala Asn Arg Ala Gly Lys Pro Ala Ile Thr
85 90 95
Trp Glu Gln Val Gln Ser Trp Phe Glu Asp Arg Gln Lys Glu Ser Arg
100 105 110
Pro Arg Val Ser Pro Ser Pro Leu Ser Leu Lys Leu Phe Val Asp Leu
115 120 125
Ser Asn Ala Lys Ile Ser Ser Asp Ala Pro Glu Ser Ser Arg Asn Ser
130 135 140
Lys Gly Lys Val Thr Asp Leu Ser Glu Leu Ile Phe Glu Ala Arg Ser
145 150 155 160
Ser Arg Asp Asn Ala Trp Tyr Asp Val Ala Ala Phe Leu Asn Tyr Arg
165 170 175
Val Leu Ser Thr Gly Glu Leu Glu Ala Arg Val Arg Phe Ser Gly Phe
180 185 190
Arg Asn Thr Asp Asp Glu Trp Val Asn Val Lys Arg Ala Val Arg Glu
195 200 205
Arg Ser Ile Pro Leu Glu Pro Ser Glu Cys His Arg Val Lys Val Gly
210 215 220
Asp Leu Val Leu Cys Phe Arg Glu Arg Phe Asp Gln Ala Val Tyr Cys
225 230 235 240
Asp Ala His Val Val Gly Ile Gln Arg Arg Pro His Glu Ala Ala Ser
245 250 255
Cys Arg Cys Ile Phe Val Val Arg Tyr Asp His Asp Asn Thr Glu Glu
260 265 270
Ala Ala Gln Leu Glu Arg Leu Cys Cys Arg Pro Thr Gln
275 280 285
<210> 3
<211> 273
<212> PRT
<213> 大豆(Glycine max)
<400> 3
Met Glu Lys Leu Pro Leu Thr Glu Tyr Pro Phe Pro Lys Leu Ser Leu
1 5 10 15
Asp Glu Ile Leu Glu Leu Glu Arg Ile Tyr Lys Asp Val Val Gly Lys
20 25 30
Val Phe Asn Arg Lys Leu Cys Gln Glu Ile Ala Lys Arg Phe Ser Ser
35 40 45
Ser Ser Asn Gly Ala Gly Lys Asn Ser Leu Ser Trp Gln Gln Val Gln
50 55 60
Leu Trp Phe Arg Asn Ser Gln Arg Met Leu Leu Gly Glu Asp Ile Ser
65 70 75 80
Ser Ser Asp Leu Leu Lys Ile Ser Ala Asp Leu Ala Asp Ser Pro Leu
85 90 95
Leu Gly Asn Gly Lys Gly Lys Gln Ala Thr Asp Leu Asp Asp Met Gly
100 105 110
Phe Glu Ala Arg Ser Thr Lys Asp Asn Ala Trp His Asp Val Ser Met
115 120 125
Phe Leu Asn Tyr Arg Val Leu Ser Thr Gly Glu Leu Glu Val Arg Val
130 135 140
Arg Tyr Ala Gly Phe Gly Lys Glu Gln Asp Glu Trp Met Asn Val Lys
145 150 155 160
Leu Gly Val Arg Glu Arg Ser Ile Pro Leu Glu Pro Ser Glu Cys His
165 170 175
Lys Val Lys Asp Gly Asp Leu Val Leu Cys Phe Leu Glu Lys Glu Asp
180 185 190
Tyr Ala Leu Tyr Cys Asp Ala Arg Ile Val Lys Ile Gln Arg Lys Ile
195 200 205
His Asp Pro Thr Asp Cys Thr Cys Thr Phe Ile Val Gln Phe Val His
210 215 220
Asp Asn Thr Glu Glu Gly Val Ser Phe Ser Arg Ile Cys Cys Arg Pro
225 230 235 240
Thr Gln Glu Glu Ser Ala Cys Phe Pro Asp Leu Ala Leu Asp Pro Thr
245 250 255
Gln Ser Pro Ser Leu Asn Phe Pro Leu Asn Thr Ile Glu Ser Leu Trp
260 265 270
Gly
<210> 4
<211> 381
<212> PRT
<213> 玉蜀黍(Zea mays)
<400> 4
Met Gly Arg Pro Pro Ser Thr Thr Gly Ala Pro Ala Phe Arg Phe Leu
1 5 10 15
Pro Ser Glu Val Ala Glu Met Glu Ala Arg Leu Leu Gln Leu Asn Asn
20 25 30
Gly Ile Pro Thr Arg Ala Met Leu Gln Thr Leu Ala Asp Lys Phe Ser
35 40 45
Ala Ser Pro Glu Arg Ala Gly Lys Val Ala Ile Gln Pro Lys Gln Val
50 55 60
Trp Asn Trp Phe Gln Asn Arg Arg Tyr Ser His Arg Ala Lys Thr Thr
65 70 75 80
Arg Val Val Pro Ser Pro Pro Ala Lys Met Pro Ser Gly Ala Asp His
85 90 95
His Gln His Ala Ala Asn Ala Ser Ala Phe Arg Ala Ala Gln Pro Ser
100 105 110
Ser Val Ala Val Ala His His Gly Ser Ser Ser Thr Gly Lys Asn Pro
115 120 125
Met Glu Gly Val Ser Val Glu Phe Glu Ala Lys Ser Ala Arg Asp Gly
130 135 140
Ala Trp Tyr Asp Val Ala Ala Phe Leu Ser His Arg Leu Phe Glu Ser
145 150 155 160
Gly Asp Pro Glu Val Arg Val Arg Phe Ser Gly Phe Gly Ala Glu Glu
165 170 175
Asp Glu Trp Ile Asn Val Arg Lys Cys Val Arg Gln Arg Ser Leu Pro
180 185 190
Cys Glu Ala Thr Glu Cys Val Ala Val Leu Pro Gly Asp Leu Ile Leu
195 200 205
Cys Phe Gln Glu Asp Lys Glu Gln Ala Leu Tyr Phe Asp Ala Arg Val
210 215 220
Leu Asp Ala Gln Arg Arg Arg His Asp Val Arg Gly Cys Arg Cys Arg
225 230 235 240
Phe Leu Val Arg Tyr Asp His Asp Ser Ser Glu Glu Ile Val Pro Leu
245 250 255
Arg Lys Val Cys Arg Arg Pro Glu Thr Asp Tyr Arg Leu Gln Ile Leu
260 265 270
His Ala Ala Arg Ala Ala Ala Ala Ser Ala Asp Ile His Thr Pro Pro
275 280 285
Arg Glu Ala Lys Val Glu Leu Ala Ser Asn Ala Lys Ser Pro Tyr Glu
290 295 300
Gln Lys Pro Gln Lys Gln Tyr Lys Met Met Asp Val Asn Thr Asp Glu
305 310 315 320
Glu Val Thr Met Val Ser Thr Leu Glu Gln Asp Glu Thr Gln Gly Lys
325 330 335
Thr Val Ala Pro Val Pro Ser Ala Thr Ala Glu Thr Arg Asn Ser Ser
340 345 350
Ser Asp Ala Val Met Lys Asp Ala Glu Ala Ser Ser Val Ala Gln Asp
355 360 365
Asp Asp Glu Val Gln Val Val Asp Lys Met Lys Glu Gly
370 375 380
<210> 5
<211> 270
<212> PRT
<213> 蒺藜苜蓿(Medicago truncatula)
<400> 5
Met Lys Glu Leu Thr Ser Asn Glu Ser Pro Phe Pro Lys Leu Ser Leu
1 5 10 15
Asp Glu Ile Leu Glu Leu Glu Arg Ile Tyr Asn Asp Val Gly Glu Lys
20 25 30
Ser Leu Asp Pro Asn Phe Cys Lys Asp Ile Ala Ala Asn Phe Ser Ser
35 40 45
Ser Ser Asn Ser Asp Gly Lys Thr Ser Leu Thr Trp Glu Gln Val Gln
50 55 60
Gln Trp Leu Gln Asn Lys His Thr Glu Thr Lys Gly His Phe Ala Ser
65 70 75 80
Ser Pro Glu Gly Leu Asn Leu Val Val Asp Leu Ser Gly Lys Ser Ser
85 90 95
Ser Ile Lys Gly Asn Lys Ser Ser Pro Lys Pro Lys Gly Ile Gln Ala
100 105 110
Ala Asp Leu Ser Glu Leu Ala Phe Glu Ala Val Ser Ile Lys Asp Asn
115 120 125
Ala Trp His Asp Val Ser Met Phe Leu Asn Tyr Arg Val Leu Cys Thr
130 135 140
Gly Glu Leu Glu Val Arg Val Arg Tyr His Gly Phe Gly Lys Asp Glu
145 150 155 160
Asp Glu Trp Ile Asn Val Lys Tyr Gly Val Arg Gln Arg Ser Ile Pro
165 170 175
Leu Glu Ala Ser Glu Cys His Lys Val Lys Glu Gly His Leu Val Leu
180 185 190
Cys Phe His Val Lys Ser Asp Tyr Ala Leu Tyr Cys Asp Ala Ile Val
195 200 205
Leu Lys Ile Gln Arg Arg Glu His Asp Ser Glu Glu Cys Ser Cys Ile
210 215 220
Phe Thr Val Arg Phe Tyr His Asp Lys Phe Glu Glu Glu Val Arg Trp
225 230 235 240
Asp Ser Leu Cys Cys Arg Pro Thr Gln Glu Glu Ser Glu Val Pro Phe
245 250 255
Glu Leu Glu Pro Thr Met Asn Pro Ile Ala Asn Leu Trp Gly
260 265 270
<210> 6
<211> 274
<212> PRT
<213> 小立碗藓(Physcomitrella patens)
<400> 6
Met Gly Arg Thr Pro Met Ser Gly Gly Thr Ala Phe Arg Phe Leu Pro
1 5 10 15
Ala Glu Val Ala Asp Met Glu Lys Ala Leu Asp Ala Ser Asn Gly Ala
20 25 30
Thr Pro Ala Arg Ser Ile Val Glu Thr Leu Ala Asp Lys Phe Thr Asn
35 40 45
Ser Ser Glu Arg Ser Gly Gln Arg Pro Val Gln Trp Lys Gln Gln Val
50 55 60
Trp Asn Trp Phe Gln Asn Arg Arg His Ala Gln Lys Ala Lys Ile Asp
65 70 75 80
Lys Leu Lys Gly Val Gly Pro Gly Gly Glu Thr Pro Ser Ala Ala Lys
85 90 95
Ser Val Val Ile Met Leu Val Thr Val Arg Leu Cys Ile Leu Val Asp
100 105 110
Arg Val Asn Val Ile Thr Gly Ala Ala Lys Lys Pro Asn Val His Val
115 120 125
Pro Met Asp Phe Glu Ala Lys Ser Ala Arg Asp Gly Ala Trp Tyr Val
130 135 140
Tyr Asp Val Ser Ala Phe Ile Asn Lys Arg Thr Gly Glu Asn Gly Glu
145 150 155 160
Pro Glu Val Arg Val Arg Phe Ala Gly Phe Gly Ser Glu Glu Asp Glu
165 170 175
Trp Val Asn Ile Ala Thr Ala Val Arg Gln Arg Ser Leu Pro Cys Glu
180 185 190
Thr Thr Glu Cys Val Ala Val Leu Pro Gly Asp Leu Ile Leu Cys Phe
195 200 205
Gln Glu Gly Ser Glu Gln Ala Leu Tyr Phe Asp Ala Asp Ile Leu Asp
210 215 220
Val Gln Arg Arg Arg His Asp Val Arg Gly Cys Arg Cys Arg Phe Trp
225 230 235 240
Val Arg Tyr Arg His Asp Gln Thr Glu Val Glu Val Val Pro Leu Arg
245 250 255
Lys Val Cys Arg Arg Pro Glu Thr Glu Gln Arg Leu Gln Gln Ala Arg
260 265 270
Glu Ala
<210> 7
<211> 381
<212> PRT
<213> 两色蜀黍(Sorghum bicolor)
<400> 7
Met Gly Arg Pro Pro Ser Thr Thr Gly Ala Pro Ala Phe Arg Phe Leu
1 5 10 15
Pro Ser Glu Val Ala Glu Met Glu Ala Arg Leu Gln Gln Leu Asn Asn
20 25 30
Gly Ile Pro Ser Arg Gly Val Leu Gln Thr Leu Ala Asp Lys Phe Ser
35 40 45
Ala Ser Pro Glu Arg Ala Gly Lys Val Ala Ile Gln Pro Lys Gln Val
50 55 60
Trp Asn Trp Phe Gln Asn Arg Arg Tyr Ser His Arg Ala Lys Ser Thr
65 70 75 80
Arg Ala Val Pro Ser Pro Pro Ala Lys Met Thr Pro Ser Gly Ala Asp
85 90 95
His His Gln His Ala Ala Asn Ala Ser Ala Phe Arg Ala Ala Gln Pro
100 105 110
Ser Ser Val Ala Ala Ala His His Gly Ala Ser Pro Thr Gly Lys Asn
115 120 125
Pro Val Glu Gly Val Ser Val Glu Phe Glu Ala Lys Ser Ala Arg Asp
130 135 140
Gly Ala Trp Tyr Asp Val Ala Ala Phe Leu Ser Gln Arg Leu Phe Glu
145 150 155 160
Thr Gly Glu Pro Glu Val Arg Val Arg Phe Ser Gly Phe Gly Ala Glu
165 170 175
Glu Asp Glu Trp Ile Asn Val Arg Lys Cys Val Arg Gln Arg Ser Leu
180 185 190
Pro Cys Glu Ala Thr Glu Cys Val Ala Val Leu Pro Gly Asp Leu Ile
195 200 205
Leu Cys Phe Gln Glu Gly Lys Glu Gln Ala Leu Tyr Phe Asp Ala Arg
210 215 220
Val Leu Asp Ala Gln Arg Arg Arg His Asp Val Arg Gly Cys Arg Cys
225 230 235 240
Arg Phe Leu Val Arg Tyr Asp His Asp Ser Ser Glu Glu Ile Val Pro
245 250 255
Leu Arg Lys Val Cys Arg Arg Pro Asp Thr Asp Tyr Arg Leu Gln Ile
260 265 270
Leu His Ala Ala Arg Val Ala Ala Thr Asn Ala Asp Ile His Thr Pro
275 280 285
Pro Lys Glu Ile Lys Val Glu Leu Thr Ser Asn Asp Lys Ser Pro Thr
290 295 300
Glu Gln Lys Pro Gln Lys Gln His Lys Met Met Asp Val Asn Thr Asp
305 310 315 320
Glu Val Thr Met Val Ser Thr Leu Glu Gln Asp Glu Thr Gln Gly Lys
325 330 335
Thr Val Ala Pro Leu Pro Ser Ser Thr Val Glu Thr Arg Asn Ser Ser
340 345 350
Ser Asp Ala Val Met Lys Asp Ala Glu Ala Ser Ser Val Val Glu Asp
355 360 365
Asp Asp Glu Val Gln Val Val Asp Lys Met Lys Glu Gly
370 375 380
<210> 8
<211> 269
<212> PRT
<213> 稻(Oryza sativa)
<400> 8
Met Glu Arg Arg Ser Ser Val Arg Phe Ala Pro Ser Glu Ile Ala Arg
1 5 10 15
Met Glu Lys Leu Val Thr His Lys Lys Glu Gln Val Leu Asp Glu Ile
20 25 30
Phe Cys Arg Lys Leu Ala Glu Glu Phe Asn Cys Ser Pro Gly Arg Val
35 40 45
Gly Ser Lys Ala Leu Gln Ala Val Gln Val Gln Glu Trp Phe Arg Gln
50 55 60
Lys Phe Pro Ala Ser Thr Val Ile Pro Pro Cys Leu Pro Thr Gly Ser
65 70 75 80
Glu Glu Lys Ala Leu Ala Ser Gln Ala Ser Ala Pro Val Ser Glu Glu
85 90 95
Lys Pro Pro Ser Ser Glu Glu Asn Ala Leu Ala Val Asp Thr Ser Ile
100 105 110
Ser Asn Asp Ile Gly Glu Val Ser Pro Asp Leu Pro Ile Asp Asn Ile
115 120 125
Asp Lys Leu Pro Glu Ile Glu Asp Met Gln Phe Glu Ala Arg Ser Ser
130 135 140
Lys Asp Phe Ala Trp Tyr Asp Ile Ala Thr Phe Leu Ala Tyr Arg Lys
145 150 155 160
Leu Ser Ser Gly Glu Phe Glu Val Arg Val Arg Phe Gln Gly Phe Gly
165 170 175
Ala Glu Glu Asp Glu Trp Ile Asn Val Arg Lys Ala Ile Arg Leu Gln
180 185 190
Ser Ile Pro Leu Glu Ser Ser Glu Cys Lys Leu Ile Arg Glu Gly Asp
195 200 205
Leu Val Leu Cys Phe Lys Glu Ser Asn Asp Glu Ala Leu His Phe Asp
210 215 220
Ala His Val Leu Glu Ile Gln Arg Lys Gln His Asp Ile Arg Gly Cys
225 230 235 240
Arg Cys Val Phe Leu Val Glu Tyr Asp His Asp Gly Thr Gln Glu Arg
245 250 255
Val Asn Leu Arg Arg Leu Ser Arg Arg Pro Lys His Ser
260 265
<210> 9
<211> 360
<212> PRT
<213> 二穗短柄草(Brachypodium distachyon)
<400> 9
Met Gly Arg Pro Pro Ser Ser Gly Gly Pro Ala Phe Arg Phe Thr His
1 5 10 15
Ala Glu Val Ala Glu Met Glu Glu His Leu Arg His Leu Asn Asn Ala
20 25 30
Ile Pro His Arg Asp Val Ile Gln Gly Leu Ala Asp Lys Phe Thr Ala
35 40 45
Ser Pro Ala Arg Ala Gly Lys Ile Ser Val Gln Pro Lys Gln Val Trp
50 55 60
Asn Trp Phe Gln Asn Arg Arg Tyr Ser Gln Arg Asn Arg Thr Thr Arg
65 70 75 80
Gly Ala Pro Leu Pro Gln Gly Lys Met Leu Pro Thr Gly Ala Ala Asp
85 90 95
Glu His His Ser Ala Ser Phe Gly Ala Gln Ser Ala Ser Ser Val Tyr
100 105 110
Pro Thr Gly Lys Asn Ser Ser Asn Gly Gly Gln Val Asp Phe Glu Ala
115 120 125
Lys Ser Ala Arg Asp Gly Ala Trp Tyr Asp Val Ala His Phe Leu Ser
130 135 140
His Arg Leu Phe Glu Thr Gly Glu Pro Glu Val Arg Val Arg Phe Ser
145 150 155 160
Gly Phe Gly Ala Glu Glu Asp Glu Trp Ile Asn Val Arg Lys Cys Val
165 170 175
Arg Gln Arg Ser Leu Pro Cys Glu Pro Thr Glu Cys Val Ala Val Leu
180 185 190
Pro Gly Asp Leu Ile Leu Cys Phe Gln Glu Gly Lys Glu Gln Ala Leu
195 200 205
Tyr Phe Asp Ala Arg Ile Leu Asp Ala Gln Arg Arg Arg His Asp Val
210 215 220
Arg Gly Cys Arg Cys Arg Phe Leu Val Arg Tyr Asp His Asp His Ser
225 230 235 240
Glu Glu Ile Val Pro Leu Arg Lys Val Cys Arg Arg Pro Asp Asn Gly
245 250 255
Phe Arg Gln His Ile Leu His Ala Asn Arg Val Ala Ala Ser Ala Gln
260 265 270
Asp Val His Met Gly Ile Gln Ile Asp Arg Ser Ser Ala Glu Gln Lys
275 280 285
Pro Gln Lys Pro His Lys Met Met Asp Val Asn Thr Asp Glu Val Thr
290 295 300
Met Val Pro Lys Val Glu Pro Thr Asp Lys Pro Val Val Ser Leu Pro
305 310 315 320
Ala Ala Ser Ile Ser Thr Arg Gly Asp Ser Ser Ser Asp Val Thr Met
325 330 335
Lys Asp Ala Gly Ala Thr Pro Glu Val Glu Ala Ala Asp Glu Gly Gln
340 345 350
Gly Asp Lys Met Asn Ala Gly Glu
355 360
<210> 10
<211> 239
<212> PRT
<213> 毛果杨(Populus trichocarpa)
<400> 10
Met Asp Phe Glu Phe Thr Leu Ser Glu Met Leu Glu Met Glu Asn Met
1 5 10 15
Phe Lys Glu Leu Glu Glu Gly Pro Leu Ala Pro Gln Phe Cys Glu Lys
20 25 30
Leu Ala Ser Ser Phe Ser Leu Ala Pro Ser Arg Asp Gly Lys Gln Ala
35 40 45
Ile Thr Pro Arg Gln Val Lys Ser Trp Phe Gln Asp Arg Leu Lys Lys
50 55 60
Ser Gln Pro Arg Val Ala Ser Ser Asn Met Ala Leu Lys Leu Phe Ala
65 70 75 80
Asp Leu Ser Asp Ala Ser Ala Ser Phe Gly Ala Thr Glu Ser Ser Gln
85 90 95
Lys Leu Lys Gly Asn Ala Ser Asp Leu Ser Glu Leu Ile Phe Glu Ala
100 105 110
Leu Ser Ser Lys Asp Asn Ala Trp Tyr Asp Val Ala Ser Phe Leu Asn
115 120 125
Tyr Arg Val Val Cys Ser Gly Glu Leu Glu Val Arg Val Arg Phe Ala
130 135 140
Gly Phe Arg Asn Thr Asp Asp Glu Trp Val Asn Val Arg Arg Ala Val
145 150 155 160
Arg Glu Arg Ser Ile Pro Leu Glu Ser Ser Glu Cys Gln Arg Val Lys
165 170 175
Val Gly Asp Leu Val Leu Cys Phe Gln Glu Arg Glu Glu Arg Ala Val
180 185 190
Tyr Cys Asp Ala His Ile Val Glu Ile Asn Arg Lys Leu His Asp Ile
195 200 205
Asn Gly Cys Arg Cys Thr Phe Val Val Arg Tyr Asp His Asp Asp Phe
210 215 220
Glu Glu Glu Val Arg Leu Asp Arg Leu Cys Gly Arg Pro Thr Pro
225 230 235
<210> 11
<211> 247
<212> PRT
<213> 葡萄(Vitis vinifera)
<400> 11
Met Asp Asp Ala Pro Val Pro Ile Ala Cys Phe Thr Gln Ser Glu Ile
1 5 10 15
Leu Glu Met Glu Asn Leu Phe Glu Glu Phe Gly Glu Glu Thr Leu Gly
20 25 30
Gln Glu Phe Cys Gln Asp Leu Ala Thr Ser Phe Ser Ala Ser Pro Gly
35 40 45
Cys Ser Gly Asn Met Pro Val Gly Trp Lys Glu Val Arg Asp Trp Phe
50 55 60
Gln Thr Lys Gln Lys Glu Leu Val Ala Arg Val Thr Ser Ser Pro Val
65 70 75 80
Ala Pro Arg Gly Ile Asp Ala Leu Pro Glu Ala Pro Met Ser Asn Asn
85 90 95
Ala Pro Gln Asn Ser Ile Val Pro Arg Gly Asp Met Val Ala Ala Asp
100 105 110
Leu Ser Glu Leu Thr Tyr Glu Ala Lys Ser Ser Lys Asp Asp Ala Trp
115 120 125
Tyr Asp Val Ala Ala Phe Leu Thr Tyr Arg Val Leu Ser Ser Gly Glu
130 135 140
Leu Glu Ala Arg Val Arg Phe Ser Gly Phe Gly Asn Glu Glu Asp Glu
145 150 155 160
Trp Val Asn Val Lys Lys Gly Ile Arg Lys Arg Ser Ile Pro Leu Glu
165 170 175
Pro Ser Glu Cys Tyr Arg Val Arg Val Gly Asp Leu Val Leu Cys Phe
180 185 190
Gln Glu Arg Ser Asp Gln Ala Val Tyr Cys Asp Ala His Ile Ile Glu
195 200 205
Ile Gln Arg Arg Leu His Asp Ile Lys Gly Cys Arg Cys Ile Phe Val
210 215 220
Val Arg Tyr Asp His Asp His Gly Glu Glu Lys Val Asn Leu Lys Arg
225 230 235 240
Leu Cys Cys Arg Pro Thr Leu
245
<210> 12
<211> 279
<212> PRT
<213> 黄瓜(Cucumis sativus)
<400> 12
Met Glu His Arg Lys Ser Ser Lys Leu Leu Asp Asp Ser Ser Phe Glu
1 5 10 15
Phe Thr Leu Ala Glu Ile Val Glu Met Asp Asn Ile Leu Lys Asp Ser
20 25 30
Arg Asp Gln Thr Leu Gly Gln Glu Phe Phe Gln Asp Val Ala Leu His
35 40 45
Phe Ser Cys Ser Pro Trp Arg Ala Ala Lys Ser Pro Val Thr Thr Glu
50 55 60
His Val His Ala Trp Phe Glu Asn Arg Arg Lys Glu Leu Arg Ala Ser
65 70 75 80
Ser Lys Lys Ala Arg Pro Pro Pro Pro Pro Pro Ser Glu Leu Pro Pro
85 90 95
Leu Pro Thr Pro Ser Ser Pro Pro Pro Ser Pro Pro Pro Lys Leu Leu
100 105 110
Leu Tyr His Ser Glu Ser Asp Phe Leu Thr His Ala Pro Ser Ser Gly
115 120 125
Pro Pro Glu Phe Lys Gly Lys Ala Thr Asp Leu Ser Glu Leu Ala Phe
130 135 140
Glu Ala Phe Ser Ser Arg Asp His Ala Trp Tyr Asp Val Ala Ser Phe
145 150 155 160
Leu Thr Tyr Arg Val Asn Cys His Gly Glu Leu Asp Ala Arg Val Arg
165 170 175
Tyr Ala Gly Phe Thr Lys Asp Glu Asp Glu Trp Val Asn Val Gly Arg
180 185 190
Gly Val Arg Asp Arg Ser Ile Pro Leu Glu Ser Ser Glu Cys Tyr Arg
195 200 205
Val Lys Val Gly Asp Leu Val Leu Cys Phe Gln Glu Arg Gln Asp His
210 215 220
Ala Leu Tyr Phe Asp Ala His Val Val Glu Ile Gln Arg Arg Leu His
225 230 235 240
Asp Ile Gly Gly Cys Arg Cys Ile Phe Val Val Arg Tyr Glu His Asp
245 250 255
Arg His Glu Glu Lys Val His Ile Gly Arg Leu Cys Cys Arg Pro Ser
260 265 270
Ala Phe Ser Ser Asp Gln Ile
275
<210> 13
<211> 276
<212> PRT
<213> 琴叶拟南芥(Arabidopsis lyrata)
<400> 13
Met Ala Ala Pro Asp Asp Ser Ser His Tyr Phe Thr Glu Phe Thr Leu
1 5 10 15
Ala Glu Ile Arg Asp Ser Cys Phe Val Ile Leu Leu Tyr Cys Ser Ser
20 25 30
Ser Ala Lys Ile Val Asp Met Glu Asn Leu Tyr Lys Glu Leu Gly Asp
35 40 45
Gln Ser Leu His Lys Asp Phe Cys Gln Thr Val Ala Ser Thr Phe Ser
50 55 60
Phe Met Ser Ser Ser Ile Val Ser Gln Ser Cys Ser Val Asn Arg Asn
65 70 75 80
Gly Lys Ser Thr Val Thr Trp Lys Gln Ile Gln Ser Trp Phe Gln Glu
85 90 95
Lys Leu Lys Gln Gln Ser Gln Pro Lys Phe Lys Thr Leu Pro Ser Pro
100 105 110
Pro Leu Gln Ile His Asp Leu Ser Asn Pro Ser Cys Tyr Ala Ala Asn
115 120 125
Ala Thr Phe Val Gln Thr Arg Lys Gly Lys Ala Ser Asp Leu Ala Asp
130 135 140
Leu Ala Phe Glu Ala Lys Ser Ala Arg Asp Tyr Ala Trp Tyr Asp Val
145 150 155 160
Ser Ser Phe Leu Thr Tyr Arg Val Leu Arg Thr Gly Glu Leu Glu Val
165 170 175
Arg Val Arg Phe Ser Gly Phe Asp Asn Arg His Asp Glu Trp Val Asn
180 185 190
Val Lys Thr Ser Val Arg Glu Arg Ser Ile Pro Leu Glu Pro Ser Glu
195 200 205
Cys Gly Arg Val Asn Ile Gly Asp Leu Leu Leu Cys Phe Gln Glu Arg
210 215 220
Asp Asp Gln Ala Leu Tyr Cys Asp Gly His Val Val Asn Ile Lys Arg
225 230 235 240
Gly Ile His Asp His Arg Arg Cys Asn Cys Val Phe Leu Val Arg Tyr
245 250 255
Asp Leu Asp Asn Thr Glu Glu Pro Leu Gly Leu Glu Lys Ile Cys Arg
260 265 270
Arg Pro Asp Glu
275
<210> 14
<211> 651
<212> PRT
<213> 拟南芥
<400> 14
Met Ser Thr Leu Leu Pro Phe Pro Asp Leu Asn Leu Met Pro Asp Ser
1 5 10 15
Gln Ser Ser Thr Ala Gly Thr Thr Ala Gly Asp Thr Val Val Thr Gly
20 25 30
Lys Leu Glu Val Lys Ser Glu Pro Ile Glu Glu Trp Gln Thr Pro Pro
35 40 45
Ser Ser Thr Ser Asp Gln Ser Ala Asn Thr Asp Leu Ile Ala Glu Phe
50 55 60
Ile Arg Ile Ser Glu Leu Phe Arg Ser Ala Phe Lys Pro Leu Gln Val
65 70 75 80
Lys Gly Leu Asp Gly Val Ser Val Tyr Gly Leu Asp Ser Gly Ala Ile
85 90 95
Val Ala Val Pro Glu Lys Glu Asn Arg Glu Leu Ile Glu Pro Pro Pro
100 105 110
Gly Phe Lys Asp Asn Arg Val Ser Thr Val Val Val Ser Pro Lys Phe
115 120 125
Glu Arg Pro Arg Glu Leu Ala Arg Ile Ala Ile Leu Gly His Glu Gln
130 135 140
Arg Lys Glu Leu Arg Gln Val Met Lys Arg Thr Arg Met Thr Tyr Glu
145 150 155 160
Ser Leu Arg Ile His Leu Met Ala Glu Ser Met Lys Asn His Val Leu
165 170 175
Gly Gln Gly Arg Arg Arg Arg Ser Asp Met Ala Ala Ala Tyr Ile Met
180 185 190
Arg Asp Arg Gly Leu Trp Leu Asn Tyr Asp Lys His Ile Val Gly Pro
195 200 205
Val Thr Gly Val Glu Val Gly Asp Ile Phe Phe Tyr Arg Met Glu Leu
210 215 220
Cys Val Leu Gly Leu His Gly Gln Thr Gln Ala Gly Ile Asp Cys Leu
225 230 235 240
Thr Ala Glu Arg Ser Ala Thr Gly Glu Pro Ile Ala Thr Ser Ile Val
245 250 255
Val Ser Gly Gly Tyr Glu Asp Asp Glu Asp Thr Gly Asp Val Leu Val
260 265 270
Tyr Thr Gly His Gly Gly Gln Asp His Gln His Lys Gln Cys Asp Asn
275 280 285
Gln Arg Leu Val Gly Gly Asn Leu Gly Met Glu Arg Ser Met His Tyr
290 295 300
Gly Ile Glu Val Arg Val Ile Arg Gly Ile Lys Tyr Glu Asn Ser Ile
305 310 315 320
Ser Ser Lys Val Tyr Val Tyr Asp Gly Leu Tyr Lys Ile Val Asp Trp
325 330 335
Trp Phe Ala Val Gly Lys Ser Gly Phe Gly Val Phe Lys Phe Arg Leu
340 345 350
Val Arg Ile Glu Gly Gln Pro Met Met Gly Ser Ala Val Met Arg Phe
355 360 365
Ala Gln Thr Leu Arg Asn Lys Pro Ser Met Val Arg Pro Thr Gly Tyr
370 375 380
Val Ser Phe Asp Leu Ser Asn Lys Lys Glu Asn Val Pro Val Phe Leu
385 390 395 400
Tyr Asn Asp Val Asp Gly Asp Gln Glu Pro Arg His Tyr Glu Tyr Ile
405 410 415
Ala Lys Ala Val Phe Pro Pro Gly Ile Phe Gly Gln Gly Gly Ile Ser
420 425 430
Arg Thr Gly Cys Glu Cys Lys Leu Ser Cys Thr Asp Asp Cys Leu Cys
435 440 445
Ala Arg Lys Asn Gly Gly Glu Phe Ala Tyr Asp Asp Asn Gly His Leu
450 455 460
Leu Lys Gly Lys His Val Val Phe Glu Cys Gly Glu Phe Cys Thr Cys
465 470 475 480
Gly Pro Ser Cys Lys Ser Arg Val Thr Gln Lys Gly Leu Arg Asn Arg
485 490 495
Leu Glu Val Phe Arg Ser Lys Glu Thr Gly Trp Gly Val Arg Thr Leu
500 505 510
Asp Leu Ile Glu Ala Gly Ala Phe Ile Cys Glu Tyr Ala Gly Val Val
515 520 525
Val Thr Arg Leu Gln Ala Glu Ile Leu Ser Met Asn Gly Asp Val Met
530 535 540
Val Tyr Pro Gly Arg Phe Thr Asp Gln Trp Arg Asn Trp Gly Asp Leu
545 550 555 560
Ser Gln Val Tyr Pro Asp Phe Val Arg Pro Asn Tyr Pro Ser Leu Pro
565 570 575
Pro Leu Asp Phe Ser Met Asp Val Ser Arg Met Arg Asn Val Ala Cys
580 585 590
Tyr Ile Ser His Ser Lys Glu Pro Asn Val Met Val Gln Phe Val Leu
595 600 605
His Asp His Asn His Leu Met Phe Pro Arg Val Met Leu Phe Ala Leu
610 615 620
Glu Asn Ile Ser Pro Leu Ala Glu Leu Ser Leu Asp Tyr Gly Leu Ala
625 630 635 640
Asp Glu Val Asn Gly Lys Leu Ala Ile Cys Asn
645 650
<210> 15
<211> 364
<212> PRT
<213> 蓖麻
<400> 15
Met His Gln Lys Leu Glu Gly Gly Asn Leu Ala Leu Glu Arg Ser Met
1 5 10 15
His Tyr Gly Ile Glu Val Arg Val Ile Arg Gly Phe Lys Tyr Ala Gly
20 25 30
Ser Phe Thr Asn Lys Ile Tyr Val Tyr Asp Gly Leu Tyr Lys Ile His
35 40 45
Asp Cys Trp Phe Asp Val Gly Lys Ser Gly Phe Gly Val Tyr Lys Tyr
50 55 60
Lys Leu Met Arg Ile Val Gly Gln Pro Glu Met Gly Ser Ser Val Leu
65 70 75 80
Arg Phe Ala Gln Ser Leu Arg Thr Ala Pro Leu Ser Val Arg Pro Arg
85 90 95
Gly Tyr Leu Ser Leu Asp Ile Ser Asn Lys Lys Glu Asn Met Pro Ile
100 105 110
Met Leu Phe Asn Asp Ile Asp Asn Asp His Asp Pro Leu Cys Tyr Glu
115 120 125
Tyr Leu Ala Arg Thr Val Phe Pro Pro Phe Ala Phe Asn Gln Gly Ser
130 135 140
Ser Gly Thr Gly Cys Glu Cys Ile Gly Gly Cys Val Asp Gly Cys Leu
145 150 155 160
Cys Ser Met Lys Asn Gly Gly Glu Phe Ala Tyr Asp Gln Asn Gly Phe
165 170 175
Leu Leu Arg Gly Lys Pro Leu Val Phe Glu Cys Gly Ala Phe Cys Lys
180 185 190
Cys Pro Pro Ser Cys Arg Asn Arg Val Ser Gln Lys Gly Leu Lys Asn
195 200 205
Arg Leu Glu Val Phe Arg Ser Arg Glu Thr Gly Trp Gly Val Arg Ser
210 215 220
Leu Asp Leu Ile His Ala Gly Glu Phe Ile Cys Glu Tyr Ala Gly Val
225 230 235 240
Ile Leu Thr Lys Asp Gln Ala Gln Val Phe Thr Met Asn Gly Asp Ser
245 250 255
Leu Ile Tyr Pro Asn Arg Phe Ser Pro Lys Trp Ala Glu Trp Gly Asp
260 265 270
Leu Ser Gln Ile Tyr Ala Asp Tyr Val Arg Pro Thr Tyr Pro Ser Val
275 280 285
Pro Pro Leu Asp Val Ala Met Asp Val Ser Arg Met Arg Asn Val Ala
290 295 300
Cys Tyr Leu Ser His Ser Ser Thr Pro Asn Ala Met Val Gln Tyr Val
305 310 315 320
Leu Phe Asp His Asn Asn Leu Met Phe Pro His Leu Met Leu Phe Ala
325 330 335
Leu Glu Asn Ile Pro Pro Leu Arg Glu Ile Ser Leu Asp Tyr Gly Val
340 345 350
Ala Asp Glu Trp Thr Gly Lys Leu Ser Ile Cys Asn
355 360
<210> 16
<211> 709
<212> PRT
<213> 大豆
<400> 16
Met Asn Phe Leu Phe Ser Arg Glu Asn Leu Pro Pro Pro Pro Gly Ile
1 5 10 15
Pro Ile Pro Ala Thr Ile Ala Ala Ala Asn Thr Thr Pro Asn Pro Ile
20 25 30
Leu Ile Pro Lys Leu Glu Pro Phe Asp Glu Leu Leu Asn Thr His Thr
35 40 45
Pro Gln Gln Gln Gln Gln Ser Gln Asp Pro Ser Phe Ser Asn Gly Ser
50 55 60
Leu Asp Leu Asp Leu Asp Leu Asp Leu Lys Leu Val Cys Asp Glu Thr
65 70 75 80
Pro Gly Ala Phe Ser Gly Asp Pro Ser Leu Leu Ser Asp Phe Asn Arg
85 90 95
Leu Ser Gln Leu Phe Lys Thr Ala Phe Thr Asn Asn Asn Ala Val Leu
100 105 110
Asp Pro Ile Ala Gln Gly Leu Gln Gln Leu Gln Asn Asp Ala Val Ser
115 120 125
Asp Pro Leu Asp Gly Thr Thr Val Val Pro Pro Gln Ser Phe Gln Asn
130 135 140
Ser Asp Pro Gly Met Gln Gln Gln Gln Gln Tyr Pro Asn Gly Ala Val
145 150 155 160
Ser Asp Pro Asp Ser Arg Ala Ile Val Pro Val Pro Glu Glu Gly Arg
165 170 175
Ser Ser Ser Val Ala Val Thr Thr Pro Arg Gln Arg Arg Phe Lys Glu
180 185 190
Leu Val Arg Leu Thr Asp Val Gly Gly Pro Glu Gln Arg His Phe Arg
195 200 205
Asp Val Val Arg Arg Thr Arg Met Val Tyr Asp Ser Leu Arg Val Leu
210 215 220
Ala Thr Val Glu Asp Glu Gly Arg Val Asp Ala Arg Arg Gly Arg Ser
225 230 235 240
Asp Leu Arg Ala Ser Ala Val Met Arg Asn Cys Gly Leu Trp Leu Asn
245 250 255
Arg Asp Lys Arg Ile Val Gly Ala Ile Pro Gly Val Cys Ile Gly Asp
260 265 270
Val Phe Leu Tyr Arg Met Glu Leu Cys Val Val Gly Leu His Gly Gln
275 280 285
Pro Gln Ala Gly Ile Asp Tyr Leu Pro Ala Ser Met Ser Ser Asn Gly
290 295 300
Glu Pro Ile Ala Thr Ser Val Ile Val Ser Gly Gly Tyr Glu Asp Asp
305 310 315 320
Val Asp Glu Gly Asp Val Ile Ile Tyr Ser Gly His Gly Gly Gln Asp
325 330 335
Lys His Ser Arg Gln Val Phe His Gln Lys Leu Glu Gly Gly Asn Leu
340 345 350
Ala Met Glu Arg Ser Met His Tyr Gly Ile Glu Val Arg Val Ile Arg
355 360 365
Gly Val Arg Tyr Glu Gly Ala Ala Ser Ala Thr Gly Arg Leu Tyr Val
370 375 380
Tyr Asp Gly Leu Tyr Arg Ile His Glu Cys Trp Phe Asp Val Gly Lys
385 390 395 400
Ser Gly Phe Gly Val Tyr Lys Tyr Lys Leu Cys Arg Ile Asp Gly Gln
405 410 415
Ala Lys Met Gly Thr Val Val Met Lys Glu Ala Leu Met Leu Arg Lys
420 425 430
Asp Pro Leu Ser Phe Lys Pro Thr Cys Cys Leu Ser Leu Asp Val Ser
435 440 445
Asn Arg Lys Glu Asn Val Ala Val Arg Leu Phe Asn Asp Ile Asp Pro
450 455 460
Asn Tyr Asp Pro Leu Gln Tyr Glu Tyr Leu Val Lys Thr Asn Phe Pro
465 470 475 480
Gln Phe Val Phe His Gln Ser Gly Arg Gly Thr Gly Cys Glu Cys Ala
485 490 495
Asp Gly Cys Val Glu Gly Cys Phe Cys Ala Met Lys Asn Gly Gly Asp
500 505 510
Phe Pro Tyr Asn Gln Ser Gly Ile Leu Leu Arg Gly Lys Pro Leu Val
515 520 525
Phe Glu Cys Gly Pro Phe Cys Arg Cys Pro Pro His Cys Arg Asn Arg
530 535 540
Val Thr Gln Lys Gly Leu Lys Asn Arg Leu Glu Val Phe Arg Ser Arg
545 550 555 560
Glu Thr Gly Trp Gly Val Arg Ser Met Asp Leu Ile Gln Ala Gly Ala
565 570 575
Phe Ile Cys Glu Tyr Thr Gly Val Val Leu Thr Arg Glu Gln Ala Arg
580 585 590
Leu Leu Thr Met Asn Gly Asp Ser Leu Ile Tyr Pro Asn Arg Phe Thr
595 600 605
Asp Arg Trp Ala Glu Trp Gly Asp Leu Ser Met Ile Asp Ser Asn Phe
610 615 620
Val Arg Pro Ser Tyr Pro Ser Ile Pro Pro Leu Asp Phe Ala Met Asp
625 630 635 640
Val Ser Arg Met Arg Asn Val Ala Cys Tyr Met Ser His Ser Ser Thr
645 650 655
Pro Asn Val Leu Val Gln Phe Val Leu Tyr Asp His Asn Asn Leu Met
660 665 670
Phe Pro Arg Leu Met Leu Phe Ala Met Glu Ser Ile Pro Pro Met Arg
675 680 685
Glu Leu Ser Leu Asp Tyr Gly Val Ala Asp Glu Trp Thr Gly Lys Leu
690 695 700
Ser Ile Cys Asn Glu
705
<210> 17
<211> 682
<212> PRT
<213> 玉蜀黍
<400> 17
Met Asp Val Val Glu Ser Ser Pro Ser Ser Ser Pro Ile Ser Ser Pro
1 5 10 15
Ala Gly Ser Ser Glu Ser Ile Asp Leu Asn Phe Leu Pro Phe Leu Lys
20 25 30
Arg Glu Pro Lys Ser Glu Pro Ala Ser Pro Glu Leu Gly Pro Leu Pro
35 40 45
Leu Pro Ala Pro Pro Thr Gln Thr Pro Gln Gln Gln His Ala Ala Ala
50 55 60
Pro Ala Pro Glu Ala Thr Ala Pro Leu Ser Pro Ala Thr Pro Asp Leu
65 70 75 80
Ser Ser Ala Pro Val Met Ala Pro Leu Gln Ser Leu Pro Pro Asn Leu
85 90 95
Asp Glu Asp Ala Leu Leu Arg Glu Tyr Tyr Arg Leu Ala Gly Leu Tyr
100 105 110
Leu Ser Ser Ala Arg Ser Gly Ala Ile Ile Pro Ala Leu Ala Pro Glu
115 120 125
Ala Ala Ala Pro Ala Val Val Gln Ser Gly Ser Gly Ser Val Val Lys
130 135 140
Lys Arg Arg Pro Arg Ser Ser Asp Leu Val Arg Val Ser Ser Leu Gly
145 150 155 160
Val Gln Asp Gln Ile Tyr Phe Arg Asp Leu Val Arg Arg Ala Arg Ile
165 170 175
Thr Phe Glu Cys Leu Arg Gly Leu Leu Leu Arg Asp Asp Glu Arg Ala
180 185 190
Glu Ser Leu Gly Leu Ala Ala Val Ala Gly Phe Gly Val Ala Arg Asp
195 200 205
Arg Arg Arg Val Arg Ala Asp Leu Arg Ala Ala Ala Leu Met Gly Asp
210 215 220
His Asp Leu Trp Leu Asn Arg Asp Arg Arg Ile Val Gly Pro Met Pro
225 230 235 240
Gly Ile Ser Val Gly Asp Ala Phe Phe Phe Arg Met Glu Leu Cys Val
245 250 255
Leu Gly Leu His Gly Gln Val Gln Ala Gly Ile Asp Tyr Val Ser Ala
260 265 270
Gly Arg Ser Ala Ser Gly Glu Pro Ile Ala Thr Ser Ile Ile Val Ser
275 280 285
Gly Gly Tyr Glu Asp Asp Asp Asp His Gly Asp Val Leu Val Tyr Thr
290 295 300
Gly His Gly Gly Arg Asp Pro Asn Leu His Lys His Cys Val Asp Gln
305 310 315 320
Lys Leu Glu Gly Gly Asn Leu Ala Leu Glu Arg Ser Met Ala Tyr Gly
325 330 335
Ile Glu Ile Arg Val Ile Arg Ala Val Lys Ser Arg Arg Ser Pro Val
340 345 350
Gly Lys Val Tyr Phe Tyr Asp Gly Leu Tyr Lys Val Val Asp Tyr Trp
355 360 365
Leu Asp Arg Gly Lys Ser Gly Phe Gly Val Tyr Lys Tyr Arg Leu Leu
370 375 380
Arg Ile Glu Gly Gln Glu Pro Met Gly Ser Val Asn Tyr Arg Val Ala
385 390 395 400
Glu Gln Leu Lys Val Asp Val Phe Ala Ile Arg Pro Thr Gly Tyr Leu
405 410 415
Ser Phe Asp Ile Ser Met Gly Arg Asp Thr Leu Ser Val Ala Leu Phe
420 425 430
Asn Asp Val Asp Asp Asp Gln Asp Pro Leu Leu Phe Glu Tyr Leu Ala
435 440 445
Arg Pro Ile Phe Pro Thr Ser Ala Val Gln Gly Lys Phe Ala Glu Gly
450 455 460
Gly Gly Gly Gly Cys Asp Cys Ala Glu Ile Cys Ser Ile Gly Cys Asn
465 470 475 480
Cys Ala Gly Arg Asn Gly Gly Glu Phe Ala Tyr Asp Arg Thr Gly Thr
485 490 495
Leu Leu Arg Gly Lys Pro Leu Val Tyr Glu Cys Gly Pro Tyr Cys Arg
500 505 510
Cys Pro Pro Ser Cys Pro Asn Arg Val Ser Gln Lys Gly Leu Gln His
515 520 525
Arg Leu Glu Val Phe Arg Ser Arg Glu Thr Gly Trp Gly Val Arg Ser
530 535 540
Leu Asp Leu Ile Lys Ala Gly Thr Phe Ile Cys Glu Phe Ser Gly Ile
545 550 555 560
Ile Leu Thr His Gln Gln Ser Glu Val Val Ala Ala Asn Gly Asp Cys
565 570 575
Leu Val Arg Pro Ser Arg Phe Pro Pro Arg Trp Leu Asp Trp Gly Asp
580 585 590
Ile Ser Asp Val Tyr Pro Asp Tyr Val Ala Pro Asp His Pro Ser Ile
595 600 605
Pro Glu Leu Lys Phe Ala Ile Asp Val Ser Arg Ala Arg Asn Val Ala
610 615 620
Cys Tyr Phe Ser His Ser Cys Ser Pro Asn Val Phe Val Gln Phe Val
625 630 635 640
Leu Phe Asp His Tyr Asn Val Ser Tyr Pro His Leu Met Ile Phe Ala
645 650 655
Met Glu Asn Ile Pro Pro Leu Arg Glu Leu Ser Ile Asp Tyr Gly Met
660 665 670
Val Asp Glu Trp Ile Gly Gln Leu Thr Met
675 680
<210> 18
<211> 570
<212> PRT
<213> 蒺藜苜蓿
<400> 18
Met Asn Phe Gln Asp Pro Asn Pro Ser Ile Ser Arg Thr Ile Val Pro
1 5 10 15
Ile Pro Asn Glu Leu Gln Asn Tyr Asp Asn Asn Thr Leu Thr Gly Lys
20 25 30
Asp Ser Lys Ser Lys Gln Lys Leu Arg Gly Gln Asn Lys Glu Leu Val
35 40 45
His Thr Val Met Asp Ala Asp His Arg Pro Tyr Arg Glu Met Val Arg
50 55 60
Arg Thr Arg Ile Leu Phe Asp Ser Ile Arg Val Ser Gly Leu Leu Glu
65 70 75 80
Glu Glu Asn Asn Arg Lys Asn Lys Lys Asn Leu Lys Ala Ser Ser Leu
85 90 95
Val Asn Gln Thr Glu Glu Val Asn Asn Ser Arg Val Arg Asn Asp Leu
100 105 110
Ile Ala Ala Thr Leu Met Lys Asp His Gly Met Tyr Leu Tyr His Asp
115 120 125
Val Lys Ile Ile Gly Thr Val Pro Gly Val Ser Ile Gly Asp Ile Phe
130 135 140
Leu Tyr Arg Ser Glu Met Cys Val Ile Gly Leu His Gly Gln Pro Gln
145 150 155 160
Ala Gly Ile Asp Tyr Leu His Ala Ser Met Ser Ser Asn Gly Gln Pro
165 170 175
Ile Ala Thr Ser Val Val Val Ser Asp Gly Tyr Asn Asp Asp Asp Gln
180 185 190
Gly Asp Ser Ile Ile Tyr Ser Gly His Gly Asp Met Lys Gln Asp Gln
195 200 205
Lys Leu Glu Arg Gly Asn Leu Ala Met Val Thr Ser Met Gln Tyr Glu
210 215 220
Ile Asp Val Arg Val Ile Arg Gly Phe Arg Tyr Glu Gly Ala Thr Ser
225 230 235 240
Thr Thr Ser Ser Lys Val Phe Val Tyr Asp Gly Leu Tyr Lys Ile Ile
245 250 255
Glu Phe Trp Phe Glu Lys Gly Ile Ser Gly Phe Gly Val Tyr Lys Phe
260 265 270
Met Leu Ser Arg Val Gln Gly Gln Pro Lys Met Gly Ser Met Ile Leu
275 280 285
Lys Glu Ala Ser Met Leu Met Arg Gly His Leu Cys Asp Asn His Met
290 295 300
Cys Val Leu Ser Asp Asp Ile Ser Asn Gly Lys Glu Asn Ile Gly Val
305 310 315 320
Arg Leu Tyr Asn Asp Ile Asp Ser Asp Leu Tyr Pro Met Gln Phe Glu
325 330 335
Tyr Leu Pro Lys Ala Ala Phe Pro Met Phe Leu Leu Pro Gln Ser Met
340 345 350
Thr Thr Arg Lys Lys Met Arg Val Ile Glu Cys Ser Glu Cys Val Asp
355 360 365
Gly Cys Val Ser Ser Ile Met Asn Gly Asn Thr Thr Pro Tyr Ser Lys
370 375 380
Ser Gly Ile Leu Leu Lys Gly Arg Ser Leu Ile Tyr Glu Cys Gly Pro
385 390 395 400
Phe Cys Ser Cys Pro Ser His Cys Arg Asn Arg Val Thr Gln Lys Gly
405 410 415
Ile Lys Tyr Arg Leu Glu Val Phe Arg Ser Asn Glu Thr Ser Trp Gly
420 425 430
Val Arg Ser Leu Asp Pro Ile Leu Ala Gly Thr Phe Ile Cys Glu Phe
435 440 445
Thr Gly Leu Val Leu Thr Arg Glu Gln Ala Glu Ile Leu Thr Met Asp
450 455 460
Gly Glu His Asn Ser Leu Ile Ile Tyr Pro Asn Arg Phe Leu Asn Arg
465 470 475 480
Ser Thr Gln Glu Trp Gly Asp Leu Ser Met Ile Asp Ala Asn His Val
485 490 495
His Pro Ala Tyr Pro Thr Leu Asp Phe Ala Leu Asp Val Ser Met Met
500 505 510
Arg Asn Val Ala Ser Tyr Ile Ser His Ser Pro Thr Pro Asn Val Phe
515 520 525
Val Gln Leu Val Leu Phe Asp His Gly Asn Trp Met Phe Pro His Leu
530 535 540
Met Val Phe Ala Met Glu Asn Ile Pro Pro Met Arg Glu Leu Ser Leu
545 550 555 560
Asp His Gly Val Glu Ala Arg Val Ile Asp
565 570
<210> 19
<211> 533
<212> PRT
<213> 小立碗藓
<400> 19
Met Asn Phe Glu Ala Ser Leu Asn Asp Glu Ala Gln Pro Asn Asp Leu
1 5 10 15
Asp Ser Glu Leu Tyr Arg Gln Arg Arg Lys Arg Tyr Ala Asp Leu Leu
20 25 30
His Phe Tyr Gln Gln Leu Gln Glu Lys Gly Glu Ser Asn Glu Arg Ile
35 40 45
Arg Pro Asp Leu Glu Ala Thr Lys Lys Met Gln Glu Glu Lys Met Asn
50 55 60
Phe Asp Trp Lys Gly Val Gly His Val Pro Gly Ser Ile Val Gly Asp
65 70 75 80
Phe Phe Phe Tyr Arg Thr Glu Leu Phe Val Leu Gly Leu His Arg Ala
85 90 95
Met Gln Ala Gly Ile Ala Tyr Thr Glu Val Gly Gln Glu Lys Ile Gly
100 105 110
Cys Ser Ile Val Ala Ser Gly Gly Tyr Glu Asp Asp Glu Asp His Gly
115 120 125
Glu Thr Met Ile Tyr Thr Gly His Gly Gly Asn Asn Lys Ala Asp Arg
130 135 140
Arg Gln Val Lys Asp Gln Lys Pro Glu Gly Gly Asn Leu Ala Leu Leu
145 150 155 160
Asn Ser Leu Lys Tyr Lys Gln Pro Val Arg Val Ile Arg Gly His Ser
165 170 175
Asp Ile Pro Thr Ser Gln Ser Pro Ser Lys Lys Ile Tyr Ser Tyr Asp
180 185 190
Gly Leu Tyr Gln Val Val Asp Gln Ser Leu Glu Leu Gly Ala Ser Gly
195 200 205
Phe Lys Val Phe Lys Phe Lys Leu Glu Arg Leu Pro Asn Gln Arg Glu
210 215 220
Leu Gly Ser Arg Leu Val Ser Phe Val Gly Lys Leu Asn Lys Ala Pro
225 230 235 240
Ser Ile Arg Thr Gly Val Val Ile Glu Asp Leu Ser Gly Gly Gln Glu
245 250 255
Pro Ile Pro Val Ser Val Val Asn Thr Val Asp Asp Thr Arg Pro Pro
260 265 270
Ser Ser Phe Glu Tyr Thr Thr Lys Leu Arg Tyr Pro Lys Gly Val Ser
275 280 285
Leu Arg Ser Ser Thr Gly Cys Ser Cys Lys Gly Asp Ser Cys His Ser
290 295 300
Val Gly His Arg Cys Ser Cys Val Leu Lys Asn Ser Gly Lys Met Leu
305 310 315 320
Pro Tyr Asn Gln Tyr Gly His Leu Ile Arg Ala Val Pro Ala Val Tyr
325 330 335
Glu Cys Gly Ser Arg Cys Lys Cys Ser Leu Glu Cys His Asn Arg Val
340 345 350
Cys Gln Lys Gly Leu Arg Tyr Arg Leu Glu Ile Phe Lys Thr Glu Lys
355 360 365
Lys Gly Trp Ala Val Arg Ser Trp Asp Phe Ile Pro Ser Gly Gly Phe
370 375 380
Val Cys Glu Tyr Thr Gly Val Ile Met Asp Thr Lys Thr Ala Asp Glu
385 390 395 400
Leu Asp Asp Asp Asp Tyr Leu Phe Asn Leu Asp Phe Lys Gln Gly Asn
405 410 415
Glu Ala Arg Trp Gly Val Gln Arg Ser Asp Val Phe Asp Ser Asp Asp
420 425 430
Ser Asp Met Pro Pro Leu Lys Leu Ser Ser Pro Lys Tyr Val Ile Asp
435 440 445
Ala Ser Lys Phe Gly Gly Val Ala Arg Phe Val Asn His Ser Cys Thr
450 455 460
Pro Asn Leu Phe Val Gln Cys Val Leu Tyr Asp His Gly Asp Leu Asp
465 470 475 480
Leu Pro His Val Met Leu Phe Ala Gly Ser Asp Ile Ser Pro Phe Gln
485 490 495
Glu Leu Thr Tyr Asp Tyr Gly Tyr Ala Leu Asn Ser Val Tyr Asp Ser
500 505 510
His Gly Asn Leu Lys Lys Lys Asp Cys His Cys Gly Thr Arg Ser Cys
515 520 525
Arg Lys Arg Leu Tyr
530
<210> 20
<211> 710
<212> PRT
<213> 两色蜀黍
<400> 20
Met Arg Arg Glu Gly Leu Gly Asn Phe Gly Val Glu Glu Glu Glu Ala
1 5 10 15
Arg Glu Asp Gly Gln Gly Ser Leu Phe Met Glu Met Asp Val Glu Glu
20 25 30
Ser Ser Pro Ser Ser Ser Leu Met Ser Ser Pro Ala Gly Ser Ser Asp
35 40 45
Ser Ile Asp Leu Asn Phe Leu Pro Phe Leu Lys Arg Glu Pro Lys Ser
50 55 60
Glu Pro Gly Ser Pro Glu Leu Gly Pro Leu Pro Leu Pro Thr Gln Thr
65 70 75 80
Pro Pro Pro Gln His Ala Ala Ala Pro Ala Pro Ala Pro Ala Pro Glu
85 90 95
Ala Ser Val Pro Leu Pro Pro Ala Thr Pro Asp Leu Ser Ser Ala Pro
100 105 110
Val Met Ala Pro Leu Gln Ser Leu Pro Pro Asn Gln Asp Val Asp Ala
115 120 125
Val Leu Gln Glu Tyr Tyr Arg Leu Ala Ser Leu Tyr Val Ser Ser Thr
130 135 140
Gly Ser Gly Ala Ile Val Pro Ala Pro Ala Pro Glu Ala Thr Ala Pro
145 150 155 160
Ala Val Val Gln Ser Ala Ser Gly Ser Val Val Lys Lys Arg Arg Pro
165 170 175
Arg Ser Ser Asp Leu Val Arg Val Ser Ser Leu Gly Val Gln Asp Gln
180 185 190
Ile Tyr Phe Arg Asp Leu Val Arg Arg Ala Arg Ile Thr Phe Glu Cys
195 200 205
Leu Arg Gly Leu Leu Leu Arg Asp Asp Glu Arg Ala Glu Ser Leu Gly
210 215 220
Leu Pro Gly Val Ala Gly Phe Gly Val Ala Arg Glu Arg Arg Arg Val
225 230 235 240
Arg Ala Asp Leu Arg Ala Ala Ala Ile Met Ala Asp His Asp Leu Trp
245 250 255
Leu Asn Arg Asp Arg Arg Ile Val Gly Pro Leu Pro Gly Ile Ser Val
260 265 270
Gly Asp Ala Phe Phe Phe Arg Met Glu Leu Cys Val Leu Gly Leu His
275 280 285
Gly Gln Val Gln Ala Gly Ile Asp Tyr Val Ser Ala Gly Arg Ser Ala
290 295 300
Ser Gly Glu Pro Ile Ala Thr Ser Ile Ile Val Ser Gly Gly Tyr Glu
305 310 315 320
Asp Asp Asp Asp His Gly Asp Val Leu Val Tyr Thr Gly His Gly Gly
325 330 335
Arg Asp Pro Asn Leu His Lys His Cys Val Asp Gln Lys Leu Glu Gly
340 345 350
Gly Asn Leu Ala Leu Glu Arg Ser Met Ala Tyr Gly Ile Glu Ile Arg
355 360 365
Val Ile Arg Ala Val Lys Ser Arg Arg Ser Pro Val Gly Lys Val Tyr
370 375 380
Phe Tyr Asp Gly Leu Tyr Lys Val Val Asp Tyr Trp Leu Asp Arg Gly
385 390 395 400
Lys Ser Gly Phe Gly Val Tyr Lys Tyr Arg Met Leu Arg Ile Glu Gly
405 410 415
Gln Glu Pro Met Gly Thr Val Asn Tyr Gln Val Ala Glu Gln Leu Lys
420 425 430
Val Asp Val Leu Ala Ile Arg Pro Thr Gly Tyr Leu Ser Phe Asp Ile
435 440 445
Ser Met Gly Arg Glu Thr Leu Pro Val Ala Leu Phe Asn Asp Val Asp
450 455 460
Asp Asp Gln Asp Pro Leu Leu Phe Glu Tyr Leu Ala Arg Pro Ile Phe
465 470 475 480
Pro Thr Ser Ala Val Gln Gly Lys Phe Ala Glu Gly Gly Gly Gly Gly
485 490 495
Cys Asp Cys Ala Gly Ile Cys Ser Ile Gly Cys Asn Cys Ala Gly Arg
500 505 510
Asn Gly Gly Glu Phe Ala Tyr Asp Lys Thr Gly Thr Leu Leu Arg Gly
515 520 525
Lys Pro Leu Val Tyr Glu Cys Gly Pro Tyr Cys Arg Cys Pro Pro Ser
530 535 540
Cys Pro Asn Arg Val Ser Gln Lys Gly Leu Gln His Lys Leu Glu Val
545 550 555 560
Phe Arg Ser Arg Glu Thr Gly Trp Gly Val Arg Ser Leu Asp Leu Ile
565 570 575
Lys Ala Gly Thr Phe Ile Cys Glu Phe Ser Gly Ile Val Leu Thr His
580 585 590
Gln Gln Ser Glu Ile Val Ala Ala Asn Gly Asp Cys Leu Val Arg Pro
595 600 605
Ser Arg Phe Pro Pro Arg Trp Leu Asp Trp Gly Asp Ile Ser Asp Val
610 615 620
Asn Pro Asp Tyr Val Ala Pro Asn His Pro Ala Ile Pro Glu Leu Asn
625 630 635 640
Phe Ala Ile Asp Val Ser Arg Ala Arg Asn Val Ala Cys Tyr Phe Ser
645 650 655
His Ser Cys Ser Pro Asn Val Phe Val Gln Phe Val Leu Phe Asp His
660 665 670
Tyr Asn Ala Ser Tyr Pro His Leu Met Ile Phe Ala Met Glu Asn Ile
675 680 685
Pro Pro Leu Arg Glu Leu Ser Ile Asp Tyr Gly Met Val Asp Glu Trp
690 695 700
Val Gly Gln Leu Thr Met
705 710
<210> 21
<211> 684
<212> PRT
<213> 稻
<400> 21
Met Glu Met Asp Thr Ser Pro Ser Ser Ser Ala Pro Ser Ser Pro Ala
1 5 10 15
Ala Ser Ser Asp Ser Ile Asp Leu Asn Phe Leu Pro Phe Leu Lys Arg
20 25 30
Glu Pro Lys Ser Glu Pro Ala Ser Pro Glu Arg Gly Pro Leu Pro Leu
35 40 45
Pro Ala Ala Ala Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro
50 55 60
Pro Pro Gln Val Gln Ala Ala Thr Val Ala Thr Pro Val Pro Ala Thr
65 70 75 80
Pro Asp Leu Ser Ala Ala Ala Val Met Thr Pro Leu Gln Ser Leu Pro
85 90 95
Pro Asn Pro Glu Glu Glu Thr Leu Leu Ala Glu Tyr Tyr Arg Leu Ala
100 105 110
Thr Leu Tyr Leu Ser Ser Ala Gly Ala Ala Gly Val Ile Val Pro Ala
115 120 125
Ala Ala Pro Glu Ala Ser Ala Gly Ala Val Ala Gln Pro Gly Ser Gly
130 135 140
Ser Gly Ala Lys Lys Arg Arg Pro Arg Ser Ser Glu Leu Val Arg Val
145 150 155 160
Ser Ser Leu Ser Val Gln Asp Gln Ile Tyr Phe Arg Asp Leu Val Arg
165 170 175
Arg Ala Arg Ile Thr Phe Glu Ser Leu Arg Gly Ile Leu Leu Arg Asp
180 185 190
Asp Glu Arg Ala Glu Val Leu Gly Leu Thr Gly Val Pro Gly Phe Gly
195 200 205
Ala Val Asp Arg Arg Arg Val Arg Ala Asp Leu Arg Ala Ala Ala Leu
210 215 220
Met Gly Asp Arg Asp Leu Trp Leu Asn Arg Asp Arg Arg Ile Val Gly
225 230 235 240
Pro Ile Pro Gly Ile Ser Val Gly Asp Ala Phe Phe Phe Arg Met Glu
245 250 255
Leu Cys Val Leu Gly Leu His Gly Gln Val Gln Ala Gly Ile Asp Phe
260 265 270
Val Thr Ala Gly Gln Ser Ser Ser Gly Glu Pro Ile Ala Thr Ser Ile
275 280 285
Ile Val Ser Gly Gly Tyr Glu Asp Asp Asp Asp Arg Gly Asp Val Leu
290 295 300
Val Tyr Thr Gly His Gly Gly Arg Asp Pro Asn Leu His Lys His Cys
305 310 315 320
Val Asp Gln Lys Leu Glu Gly Gly Asn Leu Ala Leu Glu Arg Ser Met
325 330 335
Ala Tyr Gly Ile Glu Ile Arg Val Ile Arg Ala Val Lys Ser Lys Arg
340 345 350
Ser Pro Val Gly Lys Val Tyr Phe Tyr Asp Gly Leu Tyr Lys Val Val
355 360 365
Asp Tyr Trp Leu Asp Arg Gly Lys Ser Gly Phe Gly Val Tyr Lys Tyr
370 375 380
Lys Met Leu Arg Ile Glu Gly Gln Glu Ser Met Gly Ser Val Asn Phe
385 390 395 400
Arg Leu Ala Glu Gln Leu Lys Val Asn Ala Leu Thr Phe Arg Pro Thr
405 410 415
Gly Tyr Leu Ser Phe Asp Ile Ser Met Gly Arg Glu Ile Met Pro Val
420 425 430
Ala Leu Tyr Asn Asp Val Asp Asp Asp Arg Asp Pro Leu Leu Phe Glu
435 440 445
Tyr Leu Ala Arg Pro Ile Phe Pro Ser Ser Ala Val Gln Gly Lys Phe
450 455 460
Ala Glu Gly Gly Gly Gly Cys Glu Cys Thr Glu Asn Cys Ser Ile Gly
465 470 475 480
Cys Tyr Cys Ala Gln Arg Asn Gly Gly Glu Phe Ala Tyr Asp Lys Leu
485 490 495
Gly Ala Leu Leu Arg Gly Lys Pro Leu Val Tyr Glu Cys Gly Pro Tyr
500 505 510
Cys Arg Cys Pro Pro Ser Cys Pro Asn Arg Val Ser Gln Lys Gly Leu
515 520 525
Arg Asn Arg Leu Glu Val Phe Arg Ser Arg Glu Thr Gly Trp Gly Val
530 535 540
Arg Ser Leu Asp Leu Ile Lys Ala Gly Thr Phe Ile Cys Glu Phe Ser
545 550 555 560
Gly Ile Val Leu Thr His Gln Gln Ser Glu Ile Met Ala Ala Asn Gly
565 570 575
Asp Cys Leu Val Arg Pro Ser Arg Phe Pro Pro Arg Trp Leu Asp Trp
580 585 590
Gly Asp Val Ser Asp Val Tyr Pro Glu Tyr Val Ala Pro Asn Asn Pro
595 600 605
Ala Val Pro Asp Leu Lys Phe Ser Ile Asp Val Ser Arg Ala Arg Asn
610 615 620
Val Ala Cys Tyr Phe Ser His Ser Cys Ser Pro Asn Val Phe Val Gln
625 630 635 640
Phe Val Leu Phe Asp His Tyr Asn Ala Ala Tyr Pro His Leu Met Ile
645 650 655
Phe Ala Met Glu Asn Ile Pro Pro Leu Arg Glu Leu Ser Ile Asp Tyr
660 665 670
Gly Met Ile Asp Glu Trp Val Gly Lys Leu Thr Met
675 680
<210> 22
<211> 678
<212> PRT
<213> 二穗短柄草
<400> 22
Met Glu Val Glu Glu Ser Ser Pro Ser Ser Pro Leu Ser Ser Pro Ser
1 5 10 15
Ser Ser Pro Ser Ser Asp Ser Ile Asp Leu Asn Phe Leu Pro Phe Leu
20 25 30
Lys Arg Glu Pro Lys Ser Glu Pro Ala Ser Pro Glu His Asp Pro Leu
35 40 45
Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Gln Ala Ala Ala Ala Val
50 55 60
Ala Val Ala Tyr Val Asp Pro Thr Pro Ala Thr Pro Asp Leu Ser Ser
65 70 75 80
Pro Ala Val Met Thr Pro Leu Gln Ser Leu Pro Pro Asn Pro Asp Glu
85 90 95
Asp Ala Leu Phe Ala Glu Tyr Cys Arg Leu Ala Ser Leu Tyr Leu Leu
100 105 110
Ser Ala Gly Ala Gly Ala Ile Val Gln Ala Pro Thr Pro Glu Ala Ala
115 120 125
Ala Pro Ala Val Val Gln Pro Gly Ser Gly Ser Ala Val Lys Lys Arg
130 135 140
Arg Pro Arg Ser Ser Glu Leu Val Arg Val Ser Ser Leu Gly Met Arg
145 150 155 160
Asp Gln Ile Tyr Phe Arg Asp Val Val Arg Arg Ala Arg Ile Thr Phe
165 170 175
Glu Ser Leu Arg Gly Leu Leu Leu Lys Asp Asp Glu Arg Ala Glu Ala
180 185 190
Leu Gly Leu Pro Gly Val Val Gly Leu Gly Ser Thr Asp Arg Arg Arg
195 200 205
Val Arg Ala Asp Leu Arg Ala Ala Ala Leu Met Ala Asp Arg Asp Leu
210 215 220
Trp Leu Asn Arg Asp Arg Arg Ile Val Gly Pro Ile Pro Gly Ile Ser
225 230 235 240
Val Gly Asp Ala Phe Phe Phe Arg Met Glu Leu Cys Val Leu Gly Ile
245 250 255
His Gly Gln Val Gln Ala Gly Ile Asp Tyr Leu Thr Ala Gly Arg Ser
260 265 270
Ala Ser Gly Glu Pro Ile Ala Thr Ser Ile Ile Val Ser Gly Gly Tyr
275 280 285
Glu Asp Asp Asp Asp Arg Gly Asp Ile Leu Val Tyr Thr Gly His Gly
290 295 300
Gly Arg Asp Pro Asn Leu His Lys His Cys Val Asp Gln Lys Leu Glu
305 310 315 320
Gly Gly Asn Leu Ala Leu Glu Arg Ser Met Ala Tyr Gly Ile Glu Ile
325 330 335
Arg Val Ile Arg Ala Val Lys Ser Lys Arg Ser Pro Val Gly Lys Val
340 345 350
Tyr Phe Tyr Asp Gly Leu Tyr Lys Val Val Asp Phe Trp Leu Asp Arg
355 360 365
Gly Lys Ala Gly Phe Gly Val Tyr Lys Tyr Lys Met Ile Arg Ile Asp
370 375 380
Gly Gln Asp Ala Met Gly Ser Val Asn Tyr Arg Val Ala Glu Arg Leu
385 390 395 400
Lys Val Asp Ala Leu Ser Met Arg Pro Thr Gly Tyr Leu Ser Phe Asp
405 410 415
Ile Ser Met Gly Arg Glu Ser Met Pro Val Ala Leu Tyr Asn Asp Val
420 425 430
Asp Asp Asp Lys Asp Pro Leu Leu Tyr Glu Tyr Leu Ala Arg Pro Ile
435 440 445
Phe Pro Ser Ser Ala Val Gln Gly Lys Phe Ala Glu Gly Gly Gly Gly
450 455 460
Cys Glu Cys Ile Glu Asn Cys Ser Ile Gly Cys Tyr Cys Ala Gln Arg
465 470 475 480
Asn Gly Gly Glu Phe Ala Tyr Asp Lys Ala Gly Val Leu Leu Arg Gly
485 490 495
Lys Pro Leu Val Tyr Glu Cys Gly Pro Tyr Cys Arg Cys Pro Pro Ser
500 505 510
Cys Pro Asn Arg Val Ser Gln Lys Gly Leu Lys Asn Arg Leu Glu Val
515 520 525
Phe Arg Ser Arg Glu Thr Gly Trp Gly Val Arg Ser Leu Asp Leu Ile
530 535 540
Lys Ala Gly Ala Phe Ile Cys Glu Phe Ser Gly Ile Val Leu Thr His
545 550 555 560
Gln Gln Ser Glu Ile Val Ala Val Asn Gly Asp Cys Leu Val His Pro
565 570 575
Asn Arg Phe Pro Pro Arg Trp Leu Asp Trp Gly Asp Ile Ser Asp Val
580 585 590
Tyr Pro Gly Tyr Val Pro Pro Asn His Pro Ala Ile Ala Asp Leu Asn
595 600 605
Phe Ser Ile Asp Val Ser Arg Ala Arg Asn Val Ala Cys Tyr Phe Ser
610 615 620
His Ser Cys Ser Pro Asn Val Phe Ile Gln Phe Val Leu Phe Asp His
625 630 635 640
Tyr Asn Met Ser Tyr Pro His Leu Met Ile Phe Ala Leu Glu Asn Ile
645 650 655
Pro Pro Leu Arg Glu Leu Ser Ile Asp Tyr Gly Met Ile Asp Glu Trp
660 665 670
Val Gly Lys Leu Thr Met
675
<210> 23
<211> 519
<212> PRT
<213> 毛果杨
<400> 23
Arg Ser Ser Glu Leu Val Arg Val Thr Asp Leu Gly Ile Glu Asp Gln
1 5 10 15
Arg Tyr Phe Arg Asp Leu Val Arg Arg Thr Arg Met Val Tyr Asp Ser
20 25 30
Leu Arg Ile Leu Ser Ile Leu Glu Glu Glu Lys Arg Arg Gly Glu Arg
35 40 45
Leu Gly Arg Arg Ala Arg Gly Asp Leu Arg Ala Ala Ser Ala Met Arg
50 55 60
Asp Cys Gly Leu Trp Leu Asn Arg Asp Lys Arg Ile Val Gly Ser Ile
65 70 75 80
Pro Gly Val Gln Ile Gly Asp Val Phe Phe Phe Arg Met Glu Leu Cys
85 90 95
Val Met Gly Leu His Gly Gln Ala Gln Ala Gly Ile Asp Tyr Leu Pro
100 105 110
Ala Ser Gln Ser Ser Asn Arg Glu Pro Ile Ala Thr Ser Ile Ile Val
115 120 125
Ser Gly Gly Tyr Glu Asp Asp Glu Asp Ala Gly Asp Val Ile Ile Tyr
130 135 140
Thr Gly His Gly Gly Gln Asp Lys Leu Asn Arg Gln Cys Glu His Gln
145 150 155 160
Lys Leu Glu Gly Gly Asn Leu Ala Leu Glu Arg Ser Met Arg His Gly
165 170 175
Ile Glu Val Arg Val Ile Arg Gly Ile Lys His Glu Gly Ser Val Ser
180 185 190
Ser Lys Val Tyr Val Tyr Asp Gly Leu Tyr Lys Ile Leu Asp Tyr Trp
195 200 205
Phe Asp Val Gly Lys Ser Gly Phe Gly Val Tyr Lys Tyr Arg Leu Leu
210 215 220
Arg Ile Asp Gly Gln Pro Glu Met Gly Ser Ser Ile Leu Lys Phe Ala
225 230 235 240
Glu Ser Leu Arg Thr Lys Pro Leu Thr Val Arg Pro Arg Gly Tyr Leu
245 250 255
Ser Leu Asp Ile Ser Asn Lys Lys Glu Asn Met Pro Val Phe Leu Phe
260 265 270
Asn Asp Ile Asp Asn Asp His Asp Pro Leu Cys Tyr Gln Tyr Leu Glu
275 280 285
Arg Thr Val Phe Pro Val Phe Val Phe Thr Asn Gly Ser Asn Gly Thr
290 295 300
Gly Cys Asp Cys Val Ser Gly Cys Ser Asp Gly Cys Phe Cys Ala Lys
305 310 315 320
Lys Asn Gly Gly Glu Leu Ala Tyr Asp Glu Asn Gly Phe Leu Leu Lys
325 330 335
Gly Lys Pro Val Val Phe Glu Cys Gly Val Ser Cys Arg Cys Pro Pro
340 345 350
Thr Cys Arg Asn Arg Val Thr Gln Arg Gly Leu Arg Asn Arg Leu Glu
355 360 365
Val Phe Arg Ser Arg Glu Thr Gly Trp Gly Val Arg Ser Leu Asp Val
370 375 380
Ile His Ala Gly Ala Phe Ile Cys Glu Tyr Ala Gly Val Val Leu Thr
385 390 395 400
Arg Glu Gln Ala Gln Ile Phe Thr Met Asn Gly Gly Gly Leu Val Tyr
405 410 415
Pro Asn Arg Phe Ser Ala Lys Trp Ala Glu Trp Gly Asp Leu Ser Gln
420 425 430
Ile Tyr Pro Asn Tyr Thr Arg Pro Ser Tyr Pro Glu Leu Pro Pro Leu
435 440 445
Asp Phe Ala Met Asp Val Ser Lys Met Arg Asn Val Ala Cys Tyr Met
450 455 460
Ser His Ser Ser Ala Pro Asn Val Leu Val Gln Phe Val Leu Tyr Asp
465 470 475 480
His Asn Asn Leu Met Phe Pro His Ile Met Leu Phe Ala Met Glu Asn
485 490 495
Ile Pro Pro Leu Arg Glu Leu Ser Leu Asp Tyr Gly Val Ala Asp Gly
500 505 510
Trp Thr Gly Lys Leu Ala Ile
515
<210> 24
<211> 672
<212> PRT
<213> 葡萄
<400> 24
Met Gly Ser Leu Ile Pro Phe Gln Asp Leu Asn Leu Leu Pro Asp Pro
1 5 10 15
Ala Thr Ser Pro Ser Thr Ala Ala Thr Ala Ile Thr Pro Ala Leu Ile
20 25 30
Phe Pro Lys Ile Glu Pro Lys Leu Glu Pro Phe Asp Ala Pro Thr Leu
35 40 45
Pro Leu Gln Ser Phe Pro Gln Asn Pro Ser Pro Asn Phe Phe Val Asn
50 55 60
Ser Gly Gln Leu Pro Phe Val Gly Pro Gly Ser Asn Pro Asn Asp Thr
65 70 75 80
Val Phe Ser Gln Thr Pro Glu Gly Ser Pro Glu Glu Asn Asn Val Tyr
85 90 95
Ser Glu Tyr Tyr Arg Ile Ser Glu Leu Phe Arg Thr Ala Phe Ser Lys
100 105 110
Arg Met Glu Asn Leu Gly Asn Ile Glu Val Leu Asp Pro Asp Ser Arg
115 120 125
Ala Ile Val Pro Val Pro Glu Glu Thr Arg Ile Ser Asn Val Val Val
130 135 140
Ser Arg Arg Arg Asp Gln Arg Ser Ser Glu Leu Val Arg Val Thr Asp
145 150 155 160
Leu Thr Ile Asp His Val Arg Tyr Phe Arg Asp Leu Val Arg Arg Thr
165 170 175
Arg Met Leu Tyr Asp Ala Leu Arg Ile Phe Ser Met Met Glu Glu Glu
180 185 190
Lys Arg Arg Glu Val Gly Leu Ile Thr Arg Arg Ser Arg Gly Asp Leu
195 200 205
Arg Ala Ala Lys Leu Met Lys Asp Arg Gly Leu Trp Leu Asn Arg Asp
210 215 220
Lys Arg Ile Val Gly Ser Ile Pro Gly Ile Asn Ile Gly Asp Leu Phe
225 230 235 240
Leu Phe Arg Met Glu Leu Cys Val Val Gly Leu His Gly Gln Ala Gln
245 250 255
Ala Gly Ile Asp Tyr Leu Pro Gly Ser Arg Ser Ser Asn Gly Glu Pro
260 265 270
Ile Ala Thr Ser Ile Ile Val Ser Gly Gly Tyr Glu Asp Asp Gln Asp
275 280 285
Glu Gly Asp Val Leu Ile Tyr Thr Gly His Gly Gly Gln Asp Lys Phe
290 295 300
Ser Arg Gln Cys Asp His Gln Lys Leu Glu Gly Gly Asn Leu Ala Leu
305 310 315 320
Glu Arg Ser Met His Tyr Gly Ile Glu Val Arg Val Ile Arg Gly Ile
325 330 335
Lys Tyr Glu Gly Ser Val Thr Gly Lys Val Tyr Val Tyr Asp Gly Leu
340 345 350
Tyr Lys Ile His Asp Ser Trp Phe Asp Val Gly Lys Ser Gly Phe Gly
355 360 365
Val Tyr Lys Tyr Lys Leu Leu Arg Asn Glu Gly Gln Ala Glu Met Gly
370 375 380
Ser Ala Ile Leu Arg Phe Ala Glu Asn Leu Arg Val Ser Pro Leu Thr
385 390 395 400
Val Arg Pro Val Gly Tyr Leu Cys Asp Asp Leu Ser Thr Lys Lys Glu
405 410 415
Asn Ile Pro Val Phe Leu Phe Asn Asp Ile Asp Gly Asp Asn Glu Pro
420 425 430
Met Tyr Tyr Glu Tyr Leu Pro Arg Thr Val Phe Pro Leu His Ala Tyr
435 440 445
Asn Leu Gly Gly Asn Gly Ser Gly Cys Asp Cys Val Ala Gly Cys Thr
450 455 460
Asp Asp Cys Val Cys Ala Gln Arg Asn Gly Gly Glu Phe Ala Tyr Asp
465 470 475 480
Gln Asn Gly Phe Leu Leu Arg Gly Lys Pro Val Ile Phe Glu Cys Gly
485 490 495
Ser Phe Cys Arg Cys Pro Pro Thr Cys Arg Asn Arg Leu Thr Gln Lys
500 505 510
Gly Leu Arg Asn Arg Phe Glu Val Phe Arg Ser Arg Glu Thr Gly Trp
515 520 525
Gly Val Arg Ser Leu Asp Leu Ile Gln Ala Gly Ala Phe Ile Cys Glu
530 535 540
Tyr Ala Gly Val Val Leu Thr Arg Glu Gln Ala Ala Leu Phe Ser Met
545 550 555 560
Asn Gly Asp Thr Leu Ile Tyr Pro Asn Arg Phe Thr Asp Arg Trp Ala
565 570 575
Glu Trp Gly Asp Phe Ser Lys Val Tyr Ser Asp Tyr Val Arg Pro Met
580 585 590
His Pro Ser Ile Pro Pro Leu Asp Phe Ala Met Asp Val Ser Arg Met
595 600 605
Arg Asn Leu Ala Cys Tyr Met Ser His Ser Ser Cys Pro Asn Val Leu
610 615 620
Val Gln Phe Val Leu Tyr Asp His His Asn Leu Leu Phe Pro Arg Leu
625 630 635 640
Met Leu Phe Ala Met Glu Asn Ile Pro Pro Leu Arg Glu Leu Ser Leu
645 650 655
Asp Tyr Gly Val Ala Asp Glu Trp Thr Gly Lys Leu Pro Ile Cys Asn
660 665 670
<210> 25
<211> 695
<212> PRT
<213> 黄瓜
<400> 25
Met Asp Ser Pro Ile Pro Phe Gln Asp Leu Asn Leu Leu Pro Asp Pro
1 5 10 15
Ser Ser Thr Ala Val Met Thr Ala Ala Thr Ser Pro Lys Thr Ala Thr
20 25 30
Gly Ile Asn Ser Ser Phe Asn Lys Phe Val Asp Thr Gly Lys Leu Leu
35 40 45
Thr Pro Lys Leu Glu Pro Lys Leu Glu Pro Phe Asp Asp Leu Phe Glu
50 55 60
Thr Arg Glu Ser Gln Gln Pro Gln Ser Val Gln Gln Pro Phe Leu Ser
65 70 75 80
Thr Pro Ser Ser Asn Phe Phe Ser Asn Thr Asp Phe Ser Gln Thr Pro
85 90 95
Phe Ser Asp Gln Asn His Thr Pro Leu Ser Gln Ser Ser Ser Ile Ser
100 105 110
Ser Asp Lys Asp Asn Val Tyr Ser Glu Phe Tyr Arg Ile Ser Gln Leu
115 120 125
Phe Arg Ser Ala Phe Gly Lys Gly Leu Gln Ser Tyr Gly Asp Ala Asp
130 135 140
Val Glu Val Val Asp Pro Asp Ala Gln Ala Ile Val Pro Val Pro Glu
145 150 155 160
Glu Asn Gln Ile Ser Thr Val Val Val Ser Lys Arg Arg Tyr Asp Lys
165 170 175
Arg Ser Ser Glu Leu Val Arg Val Thr Asp Leu Gly Val Glu Asp Gln
180 185 190
Arg Tyr Phe Arg Asp Val Val Arg Arg Thr Arg Met Ile Phe Asp Ser
195 200 205
Leu Arg Val Leu Ser Thr Ala Glu Glu Glu Lys Ser Pro Gly Leu Met
210 215 220
Arg Arg Leu Arg Gly Asp Leu Arg Ala Ser Ser Leu Met Arg Glu Arg
225 230 235 240
Gly Leu Trp Leu Asn Arg Asp Lys Arg Ile Val Gly Ser Ile Pro Gly
245 250 255
Val His Ile Gly Asp Leu Phe Phe Phe Arg Met Glu Leu Cys Val Val
260 265 270
Gly Leu His Gly Gln Ala Gln Ala Gly Ile Asp Tyr Val Pro Ala Ser
275 280 285
Gln Ser Ser Asn Gly Glu Pro Ile Ala Thr Ser Ile Ile Val Ser Gly
290 295 300
Gly Tyr Glu Asp Asp Glu Asp Ala Gly Asp Met Ile Ile Tyr Thr Gly
305 310 315 320
His Gly Gly Gln Asp Lys Phe Ser Lys Gln Cys Met His Gln Lys Leu
325 330 335
Glu Gly Gly Asn Leu Ala Leu Glu Arg Ser Met His Tyr Gly Ile Glu
340 345 350
Val Arg Val Ile Arg Gly Met Lys Tyr Ala Gly Ser Val Ala Ser Lys
355 360 365
Ile Tyr Val Tyr Asp Gly Leu Tyr Arg Ile Leu Asp Cys Trp Phe Asp
370 375 380
Val Gly Lys Ser Gly Phe Gly Val Tyr Lys Tyr Lys Leu Leu Arg Ile
385 390 395 400
Asp Gly Gln Ala Glu Met Gly Ser Ser Ile Leu Lys Phe Ala Glu Asn
405 410 415
Leu Arg Thr Lys Pro Leu Ser Leu Arg Pro Ser Gly Tyr Leu Ser Leu
420 425 430
Asp Ile Ser Met Lys Lys Glu Ala Val Pro Val Leu Leu Phe Asn Asp
435 440 445
Ile Asp Asn Asp Gln Glu Pro Leu Tyr Tyr Glu Tyr Leu Val Arg Thr
450 455 460
Val Phe Pro Pro Phe Ala Phe His Gln Ser Gly Ser Gly Thr Gly Cys
465 470 475 480
Ser Cys Val Thr Ser Cys Val His Asp Cys Phe Cys Ala Met Lys Asn
485 490 495
Gly Gly Glu Phe Gly Tyr Asp Gln Asn Gly Phe Leu Val Arg Gly Lys
500 505 510
Pro Ile Ile Phe Glu Cys Gly Pro Phe Cys Gln Cys Pro Pro Gln Cys
515 520 525
Arg Asn Arg Val Ser Gln Lys Gly Leu Lys His Arg Leu Glu Val Phe
530 535 540
Arg Ser Arg Glu Thr Gly Trp Gly Val Arg Ser Leu Asp Leu Ile His
545 550 555 560
Ala Gly Ala Phe Ile Cys Glu Tyr Ala Gly Val Val Leu Thr Arg Glu
565 570 575
Gln Ala Gln Val Phe Ser Met Asn Gly Asp Thr Leu Ile Tyr Pro Asn
580 585 590
Arg Phe Ser Asp Arg Trp Ala Glu Trp Gly Asp Leu Ser Gln Ile Tyr
595 600 605
Ser Asn Tyr Val Arg Pro Ser Tyr Pro Ser Val Pro Pro Leu Asp Phe
610 615 620
Ala Met Asp Val Ser Arg Met Arg Asn Val Ala Cys Tyr Ile Ser His
625 630 635 640
Ser Thr Ser Pro Asn Val Leu Val Gln Phe Val Leu Tyr Asp His Asn
645 650 655
Asn Leu Met Phe Pro His Leu Met Leu Phe Ala Met Glu Asn Ile Pro
660 665 670
Pro Leu Arg Glu Leu Ser Ile Asp Tyr Gly Val Ala Asp Asp Trp Ser
675 680 685
Gly Lys Leu Ala Ile Cys Asn
690 695
<210> 26
<211> 654
<212> PRT
<213> 琴叶拟南芥
<400> 26
Met Ser Thr Val Val Pro Phe Pro Asp Leu Asn Leu Met Pro Ala Ser
1 5 10 15
Gln Ser Ser Thr Ala Gly Thr Thr Ala Gly Asp Thr Val Val Thr Gly
20 25 30
Lys Leu Glu Val Lys Ala Glu Pro Val Glu Glu Cys Gln Thr Pro Pro
35 40 45
Ser Ser Phe Ser Asp Gln Ser Ala Ser Thr Asp Leu Ile Ala Glu Phe
50 55 60
Ile Arg Ile Ser Lys Leu Phe Arg Ser Ala Phe Lys Pro His Gln Ile
65 70 75 80
Glu Gly Leu Asp Gly Val Ser Val Tyr Gly Leu Asp Ser Gly Ala Ile
85 90 95
Val Thr Val Pro Glu Glu Glu Asn Arg Glu Leu Ile Lys Pro Pro Pro
100 105 110
Gly Phe Gly Asp Asn Arg Val Ser Thr Val Val Val Ser Pro Pro Pro
115 120 125
Thr Lys Phe Glu Arg Pro Arg Glu Leu Ala Arg Ile Ala Ile Leu Gly
130 135 140
His Asp Gln Arg Lys Glu Leu Arg Gln Val Met Lys Arg Thr Arg Met
145 150 155 160
Thr Tyr Glu Ser Leu Arg Ile His Leu Met Ala Glu Ser Met Lys Asn
165 170 175
Pro Val Leu Gly Gln Gly Arg Arg Arg Arg Ser Asp Met Ala Ala Ala
180 185 190
Tyr Ile Met Arg Asp Arg Gly Leu Trp Leu Asn Tyr Asp Lys His Ile
195 200 205
Val Gly Pro Ile Ser Gly Val Glu Ile Gly Asp Ile Phe Phe Tyr Arg
210 215 220
Met Glu Leu Cys Val Val Gly Leu His Gly Gln Thr Gln Ala Gly Ile
225 230 235 240
Asp Cys Leu Thr Ala Glu Arg Ser Ala Thr Gly Glu Pro Ile Ala Thr
245 250 255
Ser Ile Val Val Ser Gly Gly Tyr Glu Asp Asp Glu Asp Thr Gly Asp
260 265 270
Val Leu Val Tyr Thr Gly His Gly Gly Gln Asp Lys Gln His Lys Gln
275 280 285
Cys Asp Asn Gln Arg Leu Val Gly Gly Asn Leu Gly Met Glu Arg Ser
290 295 300
Met His Tyr Gly Ile Glu Val Arg Val Ile Arg Gly Ile Lys Tyr Glu
305 310 315 320
Asn Ser Ile Ser Ser Lys Val Tyr Val Tyr Asp Gly Leu Tyr Lys Ile
325 330 335
Val Asp Cys Trp Phe Ala Val Gly Lys Ser Gly Phe Gly Val Phe Lys
340 345 350
Phe Arg Leu Val Arg Met Asp Gly Gln Pro Met Met Gly Ser Ala Val
355 360 365
Met Arg Phe Ala Gln Thr Leu Arg Ser Lys Pro Leu Met Val Arg Pro
370 375 380
Thr Gly Tyr Val Ser Phe Asp Leu Ser Asn Lys Lys Glu Asn Val Pro
385 390 395 400
Val Phe Leu Tyr Asn Asp Val Asp Gly Asp Gln Glu Pro Arg His Tyr
405 410 415
Glu Tyr Ile Ala Lys Ser Val Phe Pro Pro Gly Ile Phe Gly Gln Gly
420 425 430
Gly Ile Ser Arg Thr Gly Cys Asp Cys Lys Leu Ser Cys Thr Asp Asp
435 440 445
Cys Leu Cys Ala Arg Lys Asn Gly Gly Glu Phe Ala Tyr Asp Asp Asn
450 455 460
Gly His Leu Leu Arg Gly Lys Asp Val Val Phe Glu Cys Gly Glu Leu
465 470 475 480
Cys Thr Cys Gly Pro Asn Cys Lys Ser Arg Val Thr Gln Lys Gly Leu
485 490 495
Arg Asn Arg Leu Glu Val Phe Arg Ser Lys Glu Thr Gly Trp Gly Val
500 505 510
Arg Thr Leu Asp Leu Ile Glu Ala Gly Ala Phe Ile Cys Glu Tyr Ala
515 520 525
Gly Val Val Val Thr Arg His Gln Ala Glu Ile Leu Ser Met Asn Gly
530 535 540
Asp Val Met Val Tyr Pro Gly Arg Phe Thr Asp Lys Trp Arg Asn Trp
545 550 555 560
Gly Asp Leu Ser Gln Val Tyr Pro Asp Ser Val Arg Pro Asp Tyr Pro
565 570 575
Ser Leu Pro Pro Leu Asp Phe Ala Met Asp Val Ser Arg Met Arg Asn
580 585 590
Val Ala Cys Tyr Ile Ser His Ser Lys Asp Pro Asn Val Met Val Gln
595 600 605
Phe Val Leu Tyr Asp His Asn His Leu Met Phe Pro Arg Val Met Leu
610 615 620
Phe Ala Leu Glu Asn Ile Ser Pro Leu Ala Glu Leu Ser Leu Asp Tyr
625 630 635 640
Gly Leu Ala Asp Glu Val Asn Gly Lys Leu Ala Ile Cys Asn
645 650
<210> 27
<211> 650
<212> PRT
<213> 拟南芥
<400> 27
Met Gly Ser Ser His Ile Pro Leu Asp Pro Ser Leu Asn Pro Ser Pro
1 5 10 15
Ser Leu Ile Pro Lys Leu Glu Pro Val Thr Glu Ser Thr Gln Asn Leu
20 25 30
Ala Phe Gln Leu Pro Asn Thr Asn Pro Gln Ala Leu Ile Ser Ser Ala
35 40 45
Val Ser Asp Phe Asn Glu Ala Thr Asp Phe Ser Ser Asp Tyr Asn Thr
50 55 60
Val Ala Glu Ser Ala Arg Ser Ala Phe Ala Gln Arg Leu Gln Arg His
65 70 75 80
Asp Asp Val Ala Val Leu Asp Ser Leu Thr Gly Ala Ile Val Pro Val
85 90 95
Glu Glu Asn Pro Glu Pro Glu Pro Asn Pro Tyr Ser Thr Ser Asp Ser
100 105 110
Ser Pro Ser Val Ala Thr Gln Arg Pro Arg Pro Gln Pro Arg Ser Ser
115 120 125
Glu Leu Val Arg Ile Thr Asp Val Gly Pro Glu Ser Glu Arg Gln Phe
130 135 140
Arg Glu His Val Arg Lys Thr Arg Met Ile Tyr Asp Ser Leu Arg Met
145 150 155 160
Phe Leu Met Met Glu Glu Ala Lys Arg Asn Gly Val Gly Gly Arg Arg
165 170 175
Ala Arg Ala Asp Gly Lys Ala Gly Lys Ala Gly Ser Met Met Arg Asp
180 185 190
Cys Met Leu Trp Met Asn Arg Asp Lys Arg Ile Val Gly Ser Ile Pro
195 200 205
Gly Val Gln Val Gly Asp Ile Phe Phe Phe Arg Phe Glu Leu Cys Val
210 215 220
Met Gly Leu His Gly His Pro Gln Ser Gly Ile Asp Phe Leu Thr Gly
225 230 235 240
Ser Leu Ser Ser Asn Gly Glu Pro Ile Ala Thr Ser Val Ile Val Ser
245 250 255
Gly Gly Tyr Glu Asp Asp Asp Asp Gln Gly Asp Val Ile Met Tyr Thr
260 265 270
Gly Gln Gly Gly Gln Asp Arg Leu Gly Arg Gln Ala Glu His Gln Arg
275 280 285
Leu Glu Gly Gly Asn Leu Ala Met Glu Arg Ser Met Tyr Tyr Gly Ile
290 295 300
Glu Val Arg Val Ile Arg Gly Leu Lys Tyr Glu Asn Glu Val Ser Ser
305 310 315 320
Arg Val Tyr Val Tyr Asp Gly Leu Phe Arg Ile Val Asp Ser Trp Phe
325 330 335
Asp Val Gly Lys Ser Gly Phe Gly Val Phe Lys Tyr Arg Leu Glu Arg
340 345 350
Ile Glu Gly Gln Ala Glu Met Gly Ser Ser Val Leu Lys Phe Ala Arg
355 360 365
Thr Leu Lys Thr Asn Pro Leu Ser Val Arg Pro Arg Gly Tyr Ile Asn
370 375 380
Phe Asp Ile Ser Asn Gly Lys Glu Asn Val Pro Val Tyr Leu Phe Asn
385 390 395 400
Asp Ile Asp Ser Asp Gln Glu Pro Leu Tyr Tyr Glu Tyr Leu Ala Gln
405 410 415
Thr Ser Phe Pro Pro Gly Leu Phe Val Gln Gln Ser Gly Asn Ala Ser
420 425 430
Gly Cys Asp Cys Val Asn Gly Cys Gly Ser Gly Cys Leu Cys Glu Ala
435 440 445
Lys Asn Ser Gly Glu Ile Ala Tyr Asp Tyr Asn Gly Thr Leu Ile Arg
450 455 460
Gln Lys Pro Leu Ile His Glu Cys Gly Ser Ala Cys Gln Cys Pro Pro
465 470 475 480
Ser Cys Arg Asn Arg Val Thr Gln Lys Gly Leu Arg Asn Arg Leu Glu
485 490 495
Val Phe Arg Ser Leu Glu Thr Gly Trp Gly Val Arg Ser Leu Asp Val
500 505 510
Leu His Ala Gly Ala Phe Ile Cys Glu Tyr Ala Gly Val Ala Leu Thr
515 520 525
Arg Glu Gln Ala Asn Ile Leu Thr Met Asn Gly Asp Thr Leu Val Tyr
530 535 540
Pro Ala Arg Phe Ser Ser Ala Arg Trp Glu Asp Trp Gly Asp Leu Ser
545 550 555 560
Gln Val Leu Ala Asp Phe Glu Arg Pro Ser Tyr Pro Asp Ile Pro Pro
565 570 575
Val Asp Phe Ala Met Asp Val Ser Lys Met Arg Asn Val Ala Cys Tyr
580 585 590
Ile Ser His Ser Thr Asp Pro Asn Val Ile Val Gln Phe Val Leu His
595 600 605
Asp His Asn Ser Leu Met Phe Pro Arg Val Met Leu Phe Ala Ala Glu
610 615 620
Asn Ile Pro Pro Met Thr Glu Leu Ser Leu Asp Tyr Gly Val Val Asp
625 630 635 640
Asp Trp Asn Ala Lys Leu Ala Ile Cys Asn
645 650
<210> 28
<211> 364
<212> PRT
<213> 蓖麻
<400> 28
Met His Gln Lys Leu Glu Gly Gly Asn Leu Ala Leu Glu Arg Ser Met
1 5 10 15
His Tyr Gly Ile Glu Val Arg Val Ile Arg Gly Phe Lys Tyr Ala Gly
20 25 30
Ser Phe Thr Asn Lys Ile Tyr Val Tyr Asp Gly Leu Tyr Lys Ile His
35 40 45
Asp Cys Trp Phe Asp Val Gly Lys Ser Gly Phe Gly Val Tyr Lys Tyr
50 55 60
Lys Leu Met Arg Ile Val Gly Gln Pro Glu Met Gly Ser Ser Val Leu
65 70 75 80
Arg Phe Ala Gln Ser Leu Arg Thr Ala Pro Leu Ser Val Arg Pro Arg
85 90 95
Gly Tyr Leu Ser Leu Asp Ile Ser Asn Lys Lys Glu Asn Met Pro Ile
100 105 110
Met Leu Phe Asn Asp Ile Asp Asn Asp His Asp Pro Leu Cys Tyr Glu
115 120 125
Tyr Leu Ala Arg Thr Val Phe Pro Pro Phe Ala Phe Asn Gln Gly Ser
130 135 140
Ser Gly Thr Gly Cys Glu Cys Ile Gly Gly Cys Val Asp Gly Cys Leu
145 150 155 160
Cys Ser Met Lys Asn Gly Gly Glu Phe Ala Tyr Asp Gln Asn Gly Phe
165 170 175
Leu Leu Arg Gly Lys Pro Leu Val Phe Glu Cys Gly Ala Phe Cys Lys
180 185 190
Cys Pro Pro Ser Cys Arg Asn Arg Val Ser Gln Lys Gly Leu Lys Asn
195 200 205
Arg Leu Glu Val Phe Arg Ser Arg Glu Thr Gly Trp Gly Val Arg Ser
210 215 220
Leu Asp Leu Ile His Ala Gly Glu Phe Ile Cys Glu Tyr Ala Gly Val
225 230 235 240
Ile Leu Thr Lys Asp Gln Ala Gln Val Phe Thr Met Asn Gly Asp Ser
245 250 255
Leu Ile Tyr Pro Asn Arg Phe Ser Pro Lys Trp Ala Glu Trp Gly Asp
260 265 270
Leu Ser Gln Ile Tyr Ala Asp Tyr Val Arg Pro Thr Tyr Pro Ser Val
275 280 285
Pro Pro Leu Asp Val Ala Met Asp Val Ser Arg Met Arg Asn Val Ala
290 295 300
Cys Tyr Leu Ser His Ser Ser Thr Pro Asn Ala Met Val Gln Tyr Val
305 310 315 320
Leu Phe Asp His Asn Asn Leu Met Phe Pro His Leu Met Leu Phe Ala
325 330 335
Leu Glu Asn Ile Pro Pro Leu Arg Glu Ile Ser Leu Asp Tyr Gly Val
340 345 350
Ala Asp Glu Trp Thr Gly Lys Leu Ser Ile Cys Asn
355 360
<210> 29
<211> 709
<212> PRT
<213> 大豆
<400> 29
Met Asn Phe Leu Phe Ser Arg Glu Asn Leu Pro Pro Pro Pro Gly Ile
1 5 10 15
Pro Ile Pro Ala Thr Ile Ala Ala Ala Asn Thr Thr Pro Asn Pro Ile
20 25 30
Leu Ile Pro Lys Leu Glu Pro Phe Asp Glu Leu Leu Asn Thr His Thr
35 40 45
Pro Gln Gln Gln Gln Gln Ser Gln Asp Pro Ser Phe Ser Asn Gly Ser
50 55 60
Leu Asp Leu Asp Leu Asp Leu Asp Leu Lys Leu Val Cys Asp Glu Thr
65 70 75 80
Pro Gly Ala Phe Ser Gly Asp Pro Ser Leu Leu Ser Asp Phe Asn Arg
85 90 95
Leu Ser Gln Leu Phe Lys Thr Ala Phe Thr Asn Asn Asn Ala Val Leu
100 105 110
Asp Pro Ile Ala Gln Gly Leu Gln Gln Leu Gln Asn Asp Ala Val Ser
115 120 125
Asp Pro Leu Asp Gly Thr Thr Val Val Pro Pro Gln Ser Phe Gln Asn
130 135 140
Ser Asp Pro Gly Met Gln Gln Gln Gln Gln Tyr Pro Asn Gly Ala Val
145 150 155 160
Ser Asp Pro Asp Ser Arg Ala Ile Val Pro Val Pro Glu Glu Gly Arg
165 170 175
Ser Ser Ser Val Ala Val Thr Thr Pro Arg Gln Arg Arg Phe Lys Glu
180 185 190
Leu Val Arg Leu Thr Asp Val Gly Gly Pro Glu Gln Arg His Phe Arg
195 200 205
Asp Val Val Arg Arg Thr Arg Met Val Tyr Asp Ser Leu Arg Val Leu
210 215 220
Ala Thr Val Glu Asp Glu Gly Arg Val Asp Ala Arg Arg Gly Arg Ser
225 230 235 240
Asp Leu Arg Ala Ser Ala Val Met Arg Asn Cys Gly Leu Trp Leu Asn
245 250 255
Arg Asp Lys Arg Ile Val Gly Ala Ile Pro Gly Val Cys Ile Gly Asp
260 265 270
Val Phe Leu Tyr Arg Met Glu Leu Cys Val Val Gly Leu His Gly Gln
275 280 285
Pro Gln Ala Gly Ile Asp Tyr Leu Pro Ala Ser Met Ser Ser Asn Gly
290 295 300
Glu Pro Ile Ala Thr Ser Val Ile Val Ser Gly Gly Tyr Glu Asp Asp
305 310 315 320
Val Asp Glu Gly Asp Val Ile Ile Tyr Ser Gly His Gly Gly Gln Asp
325 330 335
Lys His Ser Arg Gln Val Phe His Gln Lys Leu Glu Gly Gly Asn Leu
340 345 350
Ala Met Glu Arg Ser Met His Tyr Gly Ile Glu Val Arg Val Ile Arg
355 360 365
Gly Val Arg Tyr Glu Gly Ala Ala Ser Ala Thr Gly Arg Leu Tyr Val
370 375 380
Tyr Asp Gly Leu Tyr Arg Ile His Glu Cys Trp Phe Asp Val Gly Lys
385 390 395 400
Ser Gly Phe Gly Val Tyr Lys Tyr Lys Leu Cys Arg Ile Asp Gly Gln
405 410 415
Ala Lys Met Gly Thr Val Val Met Lys Glu Ala Leu Met Leu Arg Lys
420 425 430
Asp Pro Leu Ser Phe Lys Pro Thr Cys Cys Leu Ser Leu Asp Val Ser
435 440 445
Asn Arg Lys Glu Asn Val Ala Val Arg Leu Phe Asn Asp Ile Asp Pro
450 455 460
Asn Tyr Asp Pro Leu Gln Tyr Glu Tyr Leu Val Lys Thr Asn Phe Pro
465 470 475 480
Gln Phe Val Phe His Gln Ser Gly Arg Gly Thr Gly Cys Glu Cys Ala
485 490 495
Asp Gly Cys Val Glu Gly Cys Phe Cys Ala Met Lys Asn Gly Gly Asp
500 505 510
Phe Pro Tyr Asn Gln Ser Gly Ile Leu Leu Arg Gly Lys Pro Leu Val
515 520 525
Phe Glu Cys Gly Pro Phe Cys Arg Cys Pro Pro His Cys Arg Asn Arg
530 535 540
Val Thr Gln Lys Gly Leu Lys Asn Arg Leu Glu Val Phe Arg Ser Arg
545 550 555 560
Glu Thr Gly Trp Gly Val Arg Ser Met Asp Leu Ile Gln Ala Gly Ala
565 570 575
Phe Ile Cys Glu Tyr Thr Gly Val Val Leu Thr Arg Glu Gln Ala Arg
580 585 590
Leu Leu Thr Met Asn Gly Asp Ser Leu Ile Tyr Pro Asn Arg Phe Thr
595 600 605
Asp Arg Trp Ala Glu Trp Gly Asp Leu Ser Met Ile Asp Ser Asn Phe
610 615 620
Val Arg Pro Ser Tyr Pro Ser Ile Pro Pro Leu Asp Phe Ala Met Asp
625 630 635 640
Val Ser Arg Met Arg Asn Val Ala Cys Tyr Met Ser His Ser Ser Thr
645 650 655
Pro Asn Val Leu Val Gln Phe Val Leu Tyr Asp His Asn Asn Leu Met
660 665 670
Phe Pro Arg Leu Met Leu Phe Ala Met Glu Ser Ile Pro Pro Met Arg
675 680 685
Glu Leu Ser Leu Asp Tyr Gly Val Ala Asp Glu Trp Thr Gly Lys Leu
690 695 700
Ser Ile Cys Asn Glu
705
<210> 30
<211> 682
<212> PRT
<213> 玉蜀黍
<400> 30
Met Asp Val Val Glu Ser Ser Pro Ser Ser Ser Pro Ile Ser Ser Pro
1 5 10 15
Ala Gly Ser Ser Glu Ser Ile Asp Leu Asn Phe Leu Pro Phe Leu Lys
20 25 30
Arg Glu Pro Lys Ser Glu Pro Ala Ser Pro Glu Leu Gly Pro Leu Pro
35 40 45
Leu Pro Ala Pro Pro Thr Gln Thr Pro Gln Gln Gln His Ala Ala Ala
50 55 60
Pro Ala Pro Glu Ala Thr Ala Pro Leu Ser Pro Ala Thr Pro Asp Leu
65 70 75 80
Ser Ser Ala Pro Val Met Ala Pro Leu Gln Ser Leu Pro Pro Asn Leu
85 90 95
Asp Glu Asp Ala Leu Leu Arg Glu Tyr Tyr Arg Leu Ala Gly Leu Tyr
100 105 110
Leu Ser Ser Ala Arg Ser Gly Ala Ile Ile Pro Ala Leu Ala Pro Glu
115 120 125
Ala Ala Ala Pro Ala Val Val Gln Ser Gly Ser Gly Ser Val Val Lys
130 135 140
Lys Arg Arg Pro Arg Ser Ser Asp Leu Val Arg Val Ser Ser Leu Gly
145 150 155 160
Val Gln Asp Gln Ile Tyr Phe Arg Asp Leu Val Arg Arg Ala Arg Ile
165 170 175
Thr Phe Glu Cys Leu Arg Gly Leu Leu Leu Arg Asp Asp Glu Arg Ala
180 185 190
Glu Ser Leu Gly Leu Ala Ala Val Ala Gly Phe Gly Val Ala Arg Asp
195 200 205
Arg Arg Arg Val Arg Ala Asp Leu Arg Ala Ala Ala Leu Met Gly Asp
210 215 220
His Asp Leu Trp Leu Asn Arg Asp Arg Arg Ile Val Gly Pro Met Pro
225 230 235 240
Gly Ile Ser Val Gly Asp Ala Phe Phe Phe Arg Met Glu Leu Cys Val
245 250 255
Leu Gly Leu His Gly Gln Val Gln Ala Gly Ile Asp Tyr Val Ser Ala
260 265 270
Gly Arg Ser Ala Ser Gly Glu Pro Ile Ala Thr Ser Ile Ile Val Ser
275 280 285
Gly Gly Tyr Glu Asp Asp Asp Asp His Gly Asp Val Leu Val Tyr Thr
290 295 300
Gly His Gly Gly Arg Asp Pro Asn Leu His Lys His Cys Val Asp Gln
305 310 315 320
Lys Leu Glu Gly Gly Asn Leu Ala Leu Glu Arg Ser Met Ala Tyr Gly
325 330 335
Ile Glu Ile Arg Val Ile Arg Ala Val Lys Ser Arg Arg Ser Pro Val
340 345 350
Gly Lys Val Tyr Phe Tyr Asp Gly Leu Tyr Lys Val Val Asp Tyr Trp
355 360 365
Leu Asp Arg Gly Lys Ser Gly Phe Gly Val Tyr Lys Tyr Arg Leu Leu
370 375 380
Arg Ile Glu Gly Gln Glu Pro Met Gly Ser Val Asn Tyr Arg Val Ala
385 390 395 400
Glu Gln Leu Lys Val Asp Val Phe Ala Ile Arg Pro Thr Gly Tyr Leu
405 410 415
Ser Phe Asp Ile Ser Met Gly Arg Asp Thr Leu Ser Val Ala Leu Phe
420 425 430
Asn Asp Val Asp Asp Asp Gln Asp Pro Leu Leu Phe Glu Tyr Leu Ala
435 440 445
Arg Pro Ile Phe Pro Thr Ser Ala Val Gln Gly Lys Phe Ala Glu Gly
450 455 460
Gly Gly Gly Gly Cys Asp Cys Ala Glu Ile Cys Ser Ile Gly Cys Asn
465 470 475 480
Cys Ala Gly Arg Asn Gly Gly Glu Phe Ala Tyr Asp Arg Thr Gly Thr
485 490 495
Leu Leu Arg Gly Lys Pro Leu Val Tyr Glu Cys Gly Pro Tyr Cys Arg
500 505 510
Cys Pro Pro Ser Cys Pro Asn Arg Val Ser Gln Lys Gly Leu Gln His
515 520 525
Arg Leu Glu Val Phe Arg Ser Arg Glu Thr Gly Trp Gly Val Arg Ser
530 535 540
Leu Asp Leu Ile Lys Ala Gly Thr Phe Ile Cys Glu Phe Ser Gly Ile
545 550 555 560
Ile Leu Thr His Gln Gln Ser Glu Val Val Ala Ala Asn Gly Asp Cys
565 570 575
Leu Val Arg Pro Ser Arg Phe Pro Pro Arg Trp Leu Asp Trp Gly Asp
580 585 590
Ile Ser Asp Val Tyr Pro Asp Tyr Val Ala Pro Asp His Pro Ser Ile
595 600 605
Pro Glu Leu Lys Phe Ala Ile Asp Val Ser Arg Ala Arg Asn Val Ala
610 615 620
Cys Tyr Phe Ser His Ser Cys Ser Pro Asn Val Phe Val Gln Phe Val
625 630 635 640
Leu Phe Asp His Tyr Asn Val Ser Tyr Pro His Leu Met Ile Phe Ala
645 650 655
Met Glu Asn Ile Pro Pro Leu Arg Glu Leu Ser Ile Asp Tyr Gly Met
660 665 670
Val Asp Glu Trp Ile Gly Gln Leu Thr Met
675 680
<210> 31
<211> 570
<212> PRT
<213> 蒺藜苜蓿
<400> 31
Met Asn Phe Gln Asp Pro Asn Pro Ser Ile Ser Arg Thr Ile Val Pro
1 5 10 15
Ile Pro Asn Glu Leu Gln Asn Tyr Asp Asn Asn Thr Leu Thr Gly Lys
20 25 30
Asp Ser Lys Ser Lys Gln Lys Leu Arg Gly Gln Asn Lys Glu Leu Val
35 40 45
His Thr Val Met Asp Ala Asp His Arg Pro Tyr Arg Glu Met Val Arg
50 55 60
Arg Thr Arg Ile Leu Phe Asp Ser Ile Arg Val Ser Gly Leu Leu Glu
65 70 75 80
Glu Glu Asn Asn Arg Lys Asn Lys Lys Asn Leu Lys Ala Ser Ser Leu
85 90 95
Val Asn Gln Thr Glu Glu Val Asn Asn Ser Arg Val Arg Asn Asp Leu
100 105 110
Ile Ala Ala Thr Leu Met Lys Asp His Gly Met Tyr Leu Tyr His Asp
115 120 125
Val Lys Ile Ile Gly Thr Val Pro Gly Val Ser Ile Gly Asp Ile Phe
130 135 140
Leu Tyr Arg Ser Glu Met Cys Val Ile Gly Leu His Gly Gln Pro Gln
145 150 155 160
Ala Gly Ile Asp Tyr Leu His Ala Ser Met Ser Ser Asn Gly Gln Pro
165 170 175
Ile Ala Thr Ser Val Val Val Ser Asp Gly Tyr Asn Asp Asp Asp Gln
180 185 190
Gly Asp Ser Ile Ile Tyr Ser Gly His Gly Asp Met Lys Gln Asp Gln
195 200 205
Lys Leu Glu Arg Gly Asn Leu Ala Met Val Thr Ser Met Gln Tyr Glu
210 215 220
Ile Asp Val Arg Val Ile Arg Gly Phe Arg Tyr Glu Gly Ala Thr Ser
225 230 235 240
Thr Thr Ser Ser Lys Val Phe Val Tyr Asp Gly Leu Tyr Lys Ile Ile
245 250 255
Glu Phe Trp Phe Glu Lys Gly Ile Ser Gly Phe Gly Val Tyr Lys Phe
260 265 270
Met Leu Ser Arg Val Gln Gly Gln Pro Lys Met Gly Ser Met Ile Leu
275 280 285
Lys Glu Ala Ser Met Leu Met Arg Gly His Leu Cys Asp Asn His Met
290 295 300
Cys Val Leu Ser Asp Asp Ile Ser Asn Gly Lys Glu Asn Ile Gly Val
305 310 315 320
Arg Leu Tyr Asn Asp Ile Asp Ser Asp Leu Tyr Pro Met Gln Phe Glu
325 330 335
Tyr Leu Pro Lys Ala Ala Phe Pro Met Phe Leu Leu Pro Gln Ser Met
340 345 350
Thr Thr Arg Lys Lys Met Arg Val Ile Glu Cys Ser Glu Cys Val Asp
355 360 365
Gly Cys Val Ser Ser Ile Met Asn Gly Asn Thr Thr Pro Tyr Ser Lys
370 375 380
Ser Gly Ile Leu Leu Lys Gly Arg Ser Leu Ile Tyr Glu Cys Gly Pro
385 390 395 400
Phe Cys Ser Cys Pro Ser His Cys Arg Asn Arg Val Thr Gln Lys Gly
405 410 415
Ile Lys Tyr Arg Leu Glu Val Phe Arg Ser Asn Glu Thr Ser Trp Gly
420 425 430
Val Arg Ser Leu Asp Pro Ile Leu Ala Gly Thr Phe Ile Cys Glu Phe
435 440 445
Thr Gly Leu Val Leu Thr Arg Glu Gln Ala Glu Ile Leu Thr Met Asp
450 455 460
Gly Glu His Asn Ser Leu Ile Ile Tyr Pro Asn Arg Phe Leu Asn Arg
465 470 475 480
Ser Thr Gln Glu Trp Gly Asp Leu Ser Met Ile Asp Ala Asn His Val
485 490 495
His Pro Ala Tyr Pro Thr Leu Asp Phe Ala Leu Asp Val Ser Met Met
500 505 510
Arg Asn Val Ala Ser Tyr Ile Ser His Ser Pro Thr Pro Asn Val Phe
515 520 525
Val Gln Leu Val Leu Phe Asp His Gly Asn Trp Met Phe Pro His Leu
530 535 540
Met Val Phe Ala Met Glu Asn Ile Pro Pro Met Arg Glu Leu Ser Leu
545 550 555 560
Asp His Gly Val Glu Ala Arg Val Ile Asp
565 570
<210> 32
<211> 533
<212> PRT
<213> 小立碗藓
<400> 32
Met Asn Phe Glu Ala Ser Leu Asn Asp Glu Ala Gln Pro Asn Asp Leu
1 5 10 15
Asp Ser Glu Leu Tyr Arg Gln Arg Arg Lys Arg Tyr Ala Asp Leu Leu
20 25 30
His Phe Tyr Gln Gln Leu Gln Glu Lys Gly Glu Ser Asn Glu Arg Ile
35 40 45
Arg Pro Asp Leu Glu Ala Thr Lys Lys Met Gln Glu Glu Lys Met Asn
50 55 60
Phe Asp Trp Lys Gly Val Gly His Val Pro Gly Ser Ile Val Gly Asp
65 70 75 80
Phe Phe Phe Tyr Arg Thr Glu Leu Phe Val Leu Gly Leu His Arg Ala
85 90 95
Met Gln Ala Gly Ile Ala Tyr Thr Glu Val Gly Gln Glu Lys Ile Gly
100 105 110
Cys Ser Ile Val Ala Ser Gly Gly Tyr Glu Asp Asp Glu Asp His Gly
115 120 125
Glu Thr Met Ile Tyr Thr Gly His Gly Gly Asn Asn Lys Ala Asp Arg
130 135 140
Arg Gln Val Lys Asp Gln Lys Pro Glu Gly Gly Asn Leu Ala Leu Leu
145 150 155 160
Asn Ser Leu Lys Tyr Lys Gln Pro Val Arg Val Ile Arg Gly His Ser
165 170 175
Asp Ile Pro Thr Ser Gln Ser Pro Ser Lys Lys Ile Tyr Ser Tyr Asp
180 185 190
Gly Leu Tyr Gln Val Val Asp Gln Ser Leu Glu Leu Gly Ala Ser Gly
195 200 205
Phe Lys Val Phe Lys Phe Lys Leu Glu Arg Leu Pro Asn Gln Arg Glu
210 215 220
Leu Gly Ser Arg Leu Val Ser Phe Val Gly Lys Leu Asn Lys Ala Pro
225 230 235 240
Ser Ile Arg Thr Gly Val Val Ile Glu Asp Leu Ser Gly Gly Gln Glu
245 250 255
Pro Ile Pro Val Ser Val Val Asn Thr Val Asp Asp Thr Arg Pro Pro
260 265 270
Ser Ser Phe Glu Tyr Thr Thr Lys Leu Arg Tyr Pro Lys Gly Val Ser
275 280 285
Leu Arg Ser Ser Thr Gly Cys Ser Cys Lys Gly Asp Ser Cys His Ser
290 295 300
Val Gly His Arg Cys Ser Cys Val Leu Lys Asn Ser Gly Lys Met Leu
305 310 315 320
Pro Tyr Asn Gln Tyr Gly His Leu Ile Arg Ala Val Pro Ala Val Tyr
325 330 335
Glu Cys Gly Ser Arg Cys Lys Cys Ser Leu Glu Cys His Asn Arg Val
340 345 350
Cys Gln Lys Gly Leu Arg Tyr Arg Leu Glu Ile Phe Lys Thr Glu Lys
355 360 365
Lys Gly Trp Ala Val Arg Ser Trp Asp Phe Ile Pro Ser Gly Gly Phe
370 375 380
Val Cys Glu Tyr Thr Gly Val Ile Met Asp Thr Lys Thr Ala Asp Glu
385 390 395 400
Leu Asp Asp Asp Asp Tyr Leu Phe Asn Leu Asp Phe Lys Gln Gly Asn
405 410 415
Glu Ala Arg Trp Gly Val Gln Arg Ser Asp Val Phe Asp Ser Asp Asp
420 425 430
Ser Asp Met Pro Pro Leu Lys Leu Ser Ser Pro Lys Tyr Val Ile Asp
435 440 445
Ala Ser Lys Phe Gly Gly Val Ala Arg Phe Val Asn His Ser Cys Thr
450 455 460
Pro Asn Leu Phe Val Gln Cys Val Leu Tyr Asp His Gly Asp Leu Asp
465 470 475 480
Leu Pro His Val Met Leu Phe Ala Gly Ser Asp Ile Ser Pro Phe Gln
485 490 495
Glu Leu Thr Tyr Asp Tyr Gly Tyr Ala Leu Asn Ser Val Tyr Asp Ser
500 505 510
His Gly Asn Leu Lys Lys Lys Asp Cys His Cys Gly Thr Arg Ser Cys
515 520 525
Arg Lys Arg Leu Tyr
530
<210> 33
<211> 710
<212> PRT
<213> 两色蜀黍
<400> 33
Met Arg Arg Glu Gly Leu Gly Asn Phe Gly Val Glu Glu Glu Glu Ala
1 5 10 15
Arg Glu Asp Gly Gln Gly Ser Leu Phe Met Glu Met Asp Val Glu Glu
20 25 30
Ser Ser Pro Ser Ser Ser Leu Met Ser Ser Pro Ala Gly Ser Ser Asp
35 40 45
Ser Ile Asp Leu Asn Phe Leu Pro Phe Leu Lys Arg Glu Pro Lys Ser
50 55 60
Glu Pro Gly Ser Pro Glu Leu Gly Pro Leu Pro Leu Pro Thr Gln Thr
65 70 75 80
Pro Pro Pro Gln His Ala Ala Ala Pro Ala Pro Ala Pro Ala Pro Glu
85 90 95
Ala Ser Val Pro Leu Pro Pro Ala Thr Pro Asp Leu Ser Ser Ala Pro
100 105 110
Val Met Ala Pro Leu Gln Ser Leu Pro Pro Asn Gln Asp Val Asp Ala
115 120 125
Val Leu Gln Glu Tyr Tyr Arg Leu Ala Ser Leu Tyr Val Ser Ser Thr
130 135 140
Gly Ser Gly Ala Ile Val Pro Ala Pro Ala Pro Glu Ala Thr Ala Pro
145 150 155 160
Ala Val Val Gln Ser Ala Ser Gly Ser Val Val Lys Lys Arg Arg Pro
165 170 175
Arg Ser Ser Asp Leu Val Arg Val Ser Ser Leu Gly Val Gln Asp Gln
180 185 190
Ile Tyr Phe Arg Asp Leu Val Arg Arg Ala Arg Ile Thr Phe Glu Cys
195 200 205
Leu Arg Gly Leu Leu Leu Arg Asp Asp Glu Arg Ala Glu Ser Leu Gly
210 215 220
Leu Pro Gly Val Ala Gly Phe Gly Val Ala Arg Glu Arg Arg Arg Val
225 230 235 240
Arg Ala Asp Leu Arg Ala Ala Ala Ile Met Ala Asp His Asp Leu Trp
245 250 255
Leu Asn Arg Asp Arg Arg Ile Val Gly Pro Leu Pro Gly Ile Ser Val
260 265 270
Gly Asp Ala Phe Phe Phe Arg Met Glu Leu Cys Val Leu Gly Leu His
275 280 285
Gly Gln Val Gln Ala Gly Ile Asp Tyr Val Ser Ala Gly Arg Ser Ala
290 295 300
Ser Gly Glu Pro Ile Ala Thr Ser Ile Ile Val Ser Gly Gly Tyr Glu
305 310 315 320
Asp Asp Asp Asp His Gly Asp Val Leu Val Tyr Thr Gly His Gly Gly
325 330 335
Arg Asp Pro Asn Leu His Lys His Cys Val Asp Gln Lys Leu Glu Gly
340 345 350
Gly Asn Leu Ala Leu Glu Arg Ser Met Ala Tyr Gly Ile Glu Ile Arg
355 360 365
Val Ile Arg Ala Val Lys Ser Arg Arg Ser Pro Val Gly Lys Val Tyr
370 375 380
Phe Tyr Asp Gly Leu Tyr Lys Val Val Asp Tyr Trp Leu Asp Arg Gly
385 390 395 400
Lys Ser Gly Phe Gly Val Tyr Lys Tyr Arg Met Leu Arg Ile Glu Gly
405 410 415
Gln Glu Pro Met Gly Thr Val Asn Tyr Gln Val Ala Glu Gln Leu Lys
420 425 430
Val Asp Val Leu Ala Ile Arg Pro Thr Gly Tyr Leu Ser Phe Asp Ile
435 440 445
Ser Met Gly Arg Glu Thr Leu Pro Val Ala Leu Phe Asn Asp Val Asp
450 455 460
Asp Asp Gln Asp Pro Leu Leu Phe Glu Tyr Leu Ala Arg Pro Ile Phe
465 470 475 480
Pro Thr Ser Ala Val Gln Gly Lys Phe Ala Glu Gly Gly Gly Gly Gly
485 490 495
Cys Asp Cys Ala Gly Ile Cys Ser Ile Gly Cys Asn Cys Ala Gly Arg
500 505 510
Asn Gly Gly Glu Phe Ala Tyr Asp Lys Thr Gly Thr Leu Leu Arg Gly
515 520 525
Lys Pro Leu Val Tyr Glu Cys Gly Pro Tyr Cys Arg Cys Pro Pro Ser
530 535 540
Cys Pro Asn Arg Val Ser Gln Lys Gly Leu Gln His Lys Leu Glu Val
545 550 555 560
Phe Arg Ser Arg Glu Thr Gly Trp Gly Val Arg Ser Leu Asp Leu Ile
565 570 575
Lys Ala Gly Thr Phe Ile Cys Glu Phe Ser Gly Ile Val Leu Thr His
580 585 590
Gln Gln Ser Glu Ile Val Ala Ala Asn Gly Asp Cys Leu Val Arg Pro
595 600 605
Ser Arg Phe Pro Pro Arg Trp Leu Asp Trp Gly Asp Ile Ser Asp Val
610 615 620
Asn Pro Asp Tyr Val Ala Pro Asn His Pro Ala Ile Pro Glu Leu Asn
625 630 635 640
Phe Ala Ile Asp Val Ser Arg Ala Arg Asn Val Ala Cys Tyr Phe Ser
645 650 655
His Ser Cys Ser Pro Asn Val Phe Val Gln Phe Val Leu Phe Asp His
660 665 670
Tyr Asn Ala Ser Tyr Pro His Leu Met Ile Phe Ala Met Glu Asn Ile
675 680 685
Pro Pro Leu Arg Glu Leu Ser Ile Asp Tyr Gly Met Val Asp Glu Trp
690 695 700
Val Gly Gln Leu Thr Met
705 710
<210> 34
<211> 684
<212> PRT
<213> 稻
<400> 34
Met Glu Met Asp Thr Ser Pro Ser Ser Ser Ala Pro Ser Ser Pro Ala
1 5 10 15
Ala Ser Ser Asp Ser Ile Asp Leu Asn Phe Leu Pro Phe Leu Lys Arg
20 25 30
Glu Pro Lys Ser Glu Pro Ala Ser Pro Glu Arg Gly Pro Leu Pro Leu
35 40 45
Pro Ala Ala Ala Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro
50 55 60
Pro Pro Gln Val Gln Ala Ala Thr Val Ala Thr Pro Val Pro Ala Thr
65 70 75 80
Pro Asp Leu Ser Ala Ala Ala Val Met Thr Pro Leu Gln Ser Leu Pro
85 90 95
Pro Asn Pro Glu Glu Glu Thr Leu Leu Ala Glu Tyr Tyr Arg Leu Ala
100 105 110
Thr Leu Tyr Leu Ser Ser Ala Gly Ala Ala Gly Val Ile Val Pro Ala
115 120 125
Ala Ala Pro Glu Ala Ser Ala Gly Ala Val Ala Gln Pro Gly Ser Gly
130 135 140
Ser Gly Ala Lys Lys Arg Arg Pro Arg Ser Ser Glu Leu Val Arg Val
145 150 155 160
Ser Ser Leu Ser Val Gln Asp Gln Ile Tyr Phe Arg Asp Leu Val Arg
165 170 175
Arg Ala Arg Ile Thr Phe Glu Ser Leu Arg Gly Ile Leu Leu Arg Asp
180 185 190
Asp Glu Arg Ala Glu Val Leu Gly Leu Thr Gly Val Pro Gly Phe Gly
195 200 205
Ala Val Asp Arg Arg Arg Val Arg Ala Asp Leu Arg Ala Ala Ala Leu
210 215 220
Met Gly Asp Arg Asp Leu Trp Leu Asn Arg Asp Arg Arg Ile Val Gly
225 230 235 240
Pro Ile Pro Gly Ile Ser Val Gly Asp Ala Phe Phe Phe Arg Met Glu
245 250 255
Leu Cys Val Leu Gly Leu His Gly Gln Val Gln Ala Gly Ile Asp Phe
260 265 270
Val Thr Ala Gly Gln Ser Ser Ser Gly Glu Pro Ile Ala Thr Ser Ile
275 280 285
Ile Val Ser Gly Gly Tyr Glu Asp Asp Asp Asp Arg Gly Asp Val Leu
290 295 300
Val Tyr Thr Gly His Gly Gly Arg Asp Pro Asn Leu His Lys His Cys
305 310 315 320
Val Asp Gln Lys Leu Glu Gly Gly Asn Leu Ala Leu Glu Arg Ser Met
325 330 335
Ala Tyr Gly Ile Glu Ile Arg Val Ile Arg Ala Val Lys Ser Lys Arg
340 345 350
Ser Pro Val Gly Lys Val Tyr Phe Tyr Asp Gly Leu Tyr Lys Val Val
355 360 365
Asp Tyr Trp Leu Asp Arg Gly Lys Ser Gly Phe Gly Val Tyr Lys Tyr
370 375 380
Lys Met Leu Arg Ile Glu Gly Gln Glu Ser Met Gly Ser Val Asn Phe
385 390 395 400
Arg Leu Ala Glu Gln Leu Lys Val Asn Ala Leu Thr Phe Arg Pro Thr
405 410 415
Gly Tyr Leu Ser Phe Asp Ile Ser Met Gly Arg Glu Ile Met Pro Val
420 425 430
Ala Leu Tyr Asn Asp Val Asp Asp Asp Arg Asp Pro Leu Leu Phe Glu
435 440 445
Tyr Leu Ala Arg Pro Ile Phe Pro Ser Ser Ala Val Gln Gly Lys Phe
450 455 460
Ala Glu Gly Gly Gly Gly Cys Glu Cys Thr Glu Asn Cys Ser Ile Gly
465 470 475 480
Cys Tyr Cys Ala Gln Arg Asn Gly Gly Glu Phe Ala Tyr Asp Lys Leu
485 490 495
Gly Ala Leu Leu Arg Gly Lys Pro Leu Val Tyr Glu Cys Gly Pro Tyr
500 505 510
Cys Arg Cys Pro Pro Ser Cys Pro Asn Arg Val Ser Gln Lys Gly Leu
515 520 525
Arg Asn Arg Leu Glu Val Phe Arg Ser Arg Glu Thr Gly Trp Gly Val
530 535 540
Arg Ser Leu Asp Leu Ile Lys Ala Gly Thr Phe Ile Cys Glu Phe Ser
545 550 555 560
Gly Ile Val Leu Thr His Gln Gln Ser Glu Ile Met Ala Ala Asn Gly
565 570 575
Asp Cys Leu Val Arg Pro Ser Arg Phe Pro Pro Arg Trp Leu Asp Trp
580 585 590
Gly Asp Val Ser Asp Val Tyr Pro Glu Tyr Val Ala Pro Asn Asn Pro
595 600 605
Ala Val Pro Asp Leu Lys Phe Ser Ile Asp Val Ser Arg Ala Arg Asn
610 615 620
Val Ala Cys Tyr Phe Ser His Ser Cys Ser Pro Asn Val Phe Val Gln
625 630 635 640
Phe Val Leu Phe Asp His Tyr Asn Ala Ala Tyr Pro His Leu Met Ile
645 650 655
Phe Ala Met Glu Asn Ile Pro Pro Leu Arg Glu Leu Ser Ile Asp Tyr
660 665 670
Gly Met Ile Asp Glu Trp Val Gly Lys Leu Thr Met
675 680
<210> 35
<211> 678
<212> PRT
<213> 二穗短柄草
<400> 35
Met Glu Val Glu Glu Ser Ser Pro Ser Ser Pro Leu Ser Ser Pro Ser
1 5 10 15
Ser Ser Pro Ser Ser Asp Ser Ile Asp Leu Asn Phe Leu Pro Phe Leu
20 25 30
Lys Arg Glu Pro Lys Ser Glu Pro Ala Ser Pro Glu His Asp Pro Leu
35 40 45
Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro Gln Ala Ala Ala Ala Val
50 55 60
Ala Val Ala Tyr Val Asp Pro Thr Pro Ala Thr Pro Asp Leu Ser Ser
65 70 75 80
Pro Ala Val Met Thr Pro Leu Gln Ser Leu Pro Pro Asn Pro Asp Glu
85 90 95
Asp Ala Leu Phe Ala Glu Tyr Cys Arg Leu Ala Ser Leu Tyr Leu Leu
100 105 110
Ser Ala Gly Ala Gly Ala Ile Val Gln Ala Pro Thr Pro Glu Ala Ala
115 120 125
Ala Pro Ala Val Val Gln Pro Gly Ser Gly Ser Ala Val Lys Lys Arg
130 135 140
Arg Pro Arg Ser Ser Glu Leu Val Arg Val Ser Ser Leu Gly Met Arg
145 150 155 160
Asp Gln Ile Tyr Phe Arg Asp Val Val Arg Arg Ala Arg Ile Thr Phe
165 170 175
Glu Ser Leu Arg Gly Leu Leu Leu Lys Asp Asp Glu Arg Ala Glu Ala
180 185 190
Leu Gly Leu Pro Gly Val Val Gly Leu Gly Ser Thr Asp Arg Arg Arg
195 200 205
Val Arg Ala Asp Leu Arg Ala Ala Ala Leu Met Ala Asp Arg Asp Leu
210 215 220
Trp Leu Asn Arg Asp Arg Arg Ile Val Gly Pro Ile Pro Gly Ile Ser
225 230 235 240
Val Gly Asp Ala Phe Phe Phe Arg Met Glu Leu Cys Val Leu Gly Ile
245 250 255
His Gly Gln Val Gln Ala Gly Ile Asp Tyr Leu Thr Ala Gly Arg Ser
260 265 270
Ala Ser Gly Glu Pro Ile Ala Thr Ser Ile Ile Val Ser Gly Gly Tyr
275 280 285
Glu Asp Asp Asp Asp Arg Gly Asp Ile Leu Val Tyr Thr Gly His Gly
290 295 300
Gly Arg Asp Pro Asn Leu His Lys His Cys Val Asp Gln Lys Leu Glu
305 310 315 320
Gly Gly Asn Leu Ala Leu Glu Arg Ser Met Ala Tyr Gly Ile Glu Ile
325 330 335
Arg Val Ile Arg Ala Val Lys Ser Lys Arg Ser Pro Val Gly Lys Val
340 345 350
Tyr Phe Tyr Asp Gly Leu Tyr Lys Val Val Asp Phe Trp Leu Asp Arg
355 360 365
Gly Lys Ala Gly Phe Gly Val Tyr Lys Tyr Lys Met Ile Arg Ile Asp
370 375 380
Gly Gln Asp Ala Met Gly Ser Val Asn Tyr Arg Val Ala Glu Arg Leu
385 390 395 400
Lys Val Asp Ala Leu Ser Met Arg Pro Thr Gly Tyr Leu Ser Phe Asp
405 410 415
Ile Ser Met Gly Arg Glu Ser Met Pro Val Ala Leu Tyr Asn Asp Val
420 425 430
Asp Asp Asp Lys Asp Pro Leu Leu Tyr Glu Tyr Leu Ala Arg Pro Ile
435 440 445
Phe Pro Ser Ser Ala Val Gln Gly Lys Phe Ala Glu Gly Gly Gly Gly
450 455 460
Cys Glu Cys Ile Glu Asn Cys Ser Ile Gly Cys Tyr Cys Ala Gln Arg
465 470 475 480
Asn Gly Gly Glu Phe Ala Tyr Asp Lys Ala Gly Val Leu Leu Arg Gly
485 490 495
Lys Pro Leu Val Tyr Glu Cys Gly Pro Tyr Cys Arg Cys Pro Pro Ser
500 505 510
Cys Pro Asn Arg Val Ser Gln Lys Gly Leu Lys Asn Arg Leu Glu Val
515 520 525
Phe Arg Ser Arg Glu Thr Gly Trp Gly Val Arg Ser Leu Asp Leu Ile
530 535 540
Lys Ala Gly Ala Phe Ile Cys Glu Phe Ser Gly Ile Val Leu Thr His
545 550 555 560
Gln Gln Ser Glu Ile Val Ala Val Asn Gly Asp Cys Leu Val His Pro
565 570 575
Asn Arg Phe Pro Pro Arg Trp Leu Asp Trp Gly Asp Ile Ser Asp Val
580 585 590
Tyr Pro Gly Tyr Val Pro Pro Asn His Pro Ala Ile Ala Asp Leu Asn
595 600 605
Phe Ser Ile Asp Val Ser Arg Ala Arg Asn Val Ala Cys Tyr Phe Ser
610 615 620
His Ser Cys Ser Pro Asn Val Phe Ile Gln Phe Val Leu Phe Asp His
625 630 635 640
Tyr Asn Met Ser Tyr Pro His Leu Met Ile Phe Ala Leu Glu Asn Ile
645 650 655
Pro Pro Leu Arg Glu Leu Ser Ile Asp Tyr Gly Met Ile Asp Glu Trp
660 665 670
Val Gly Lys Leu Thr Met
675
<210> 36
<211> 519
<212> PRT
<213> 毛果杨
<400> 36
Arg Ser Ser Glu Leu Val Arg Val Thr Asp Leu Gly Ile Glu Asp Gln
1 5 10 15
Arg Tyr Phe Arg Asp Leu Val Arg Arg Thr Arg Met Val Tyr Asp Ser
20 25 30
Leu Arg Ile Leu Ser Ile Leu Glu Glu Glu Lys Arg Arg Gly Glu Arg
35 40 45
Leu Gly Arg Arg Ala Arg Gly Asp Leu Arg Ala Ala Ser Ala Met Arg
50 55 60
Asp Cys Gly Leu Trp Leu Asn Arg Asp Lys Arg Ile Val Gly Ser Ile
65 70 75 80
Pro Gly Val Gln Ile Gly Asp Val Phe Phe Phe Arg Met Glu Leu Cys
85 90 95
Val Met Gly Leu His Gly Gln Ala Gln Ala Gly Ile Asp Tyr Leu Pro
100 105 110
Ala Ser Gln Ser Ser Asn Arg Glu Pro Ile Ala Thr Ser Ile Ile Val
115 120 125
Ser Gly Gly Tyr Glu Asp Asp Glu Asp Ala Gly Asp Val Ile Ile Tyr
130 135 140
Thr Gly His Gly Gly Gln Asp Lys Leu Asn Arg Gln Cys Glu His Gln
145 150 155 160
Lys Leu Glu Gly Gly Asn Leu Ala Leu Glu Arg Ser Met Arg His Gly
165 170 175
Ile Glu Val Arg Val Ile Arg Gly Ile Lys His Glu Gly Ser Val Ser
180 185 190
Ser Lys Val Tyr Val Tyr Asp Gly Leu Tyr Lys Ile Leu Asp Tyr Trp
195 200 205
Phe Asp Val Gly Lys Ser Gly Phe Gly Val Tyr Lys Tyr Arg Leu Leu
210 215 220
Arg Ile Asp Gly Gln Pro Glu Met Gly Ser Ser Ile Leu Lys Phe Ala
225 230 235 240
Glu Ser Leu Arg Thr Lys Pro Leu Thr Val Arg Pro Arg Gly Tyr Leu
245 250 255
Ser Leu Asp Ile Ser Asn Lys Lys Glu Asn Met Pro Val Phe Leu Phe
260 265 270
Asn Asp Ile Asp Asn Asp His Asp Pro Leu Cys Tyr Gln Tyr Leu Glu
275 280 285
Arg Thr Val Phe Pro Val Phe Val Phe Thr Asn Gly Ser Asn Gly Thr
290 295 300
Gly Cys Asp Cys Val Ser Gly Cys Ser Asp Gly Cys Phe Cys Ala Lys
305 310 315 320
Lys Asn Gly Gly Glu Leu Ala Tyr Asp Glu Asn Gly Phe Leu Leu Lys
325 330 335
Gly Lys Pro Val Val Phe Glu Cys Gly Val Ser Cys Arg Cys Pro Pro
340 345 350
Thr Cys Arg Asn Arg Val Thr Gln Arg Gly Leu Arg Asn Arg Leu Glu
355 360 365
Val Phe Arg Ser Arg Glu Thr Gly Trp Gly Val Arg Ser Leu Asp Val
370 375 380
Ile His Ala Gly Ala Phe Ile Cys Glu Tyr Ala Gly Val Val Leu Thr
385 390 395 400
Arg Glu Gln Ala Gln Ile Phe Thr Met Asn Gly Gly Gly Leu Val Tyr
405 410 415
Pro Asn Arg Phe Ser Ala Lys Trp Ala Glu Trp Gly Asp Leu Ser Gln
420 425 430
Ile Tyr Pro Asn Tyr Thr Arg Pro Ser Tyr Pro Glu Leu Pro Pro Leu
435 440 445
Asp Phe Ala Met Asp Val Ser Lys Met Arg Asn Val Ala Cys Tyr Met
450 455 460
Ser His Ser Ser Ala Pro Asn Val Leu Val Gln Phe Val Leu Tyr Asp
465 470 475 480
His Asn Asn Leu Met Phe Pro His Ile Met Leu Phe Ala Met Glu Asn
485 490 495
Ile Pro Pro Leu Arg Glu Leu Ser Leu Asp Tyr Gly Val Ala Asp Gly
500 505 510
Trp Thr Gly Lys Leu Ala Ile
515
<210> 37
<211> 672
<212> PRT
<213> 葡萄
<400> 37
Met Gly Ser Leu Ile Pro Phe Gln Asp Leu Asn Leu Leu Pro Asp Pro
1 5 10 15
Ala Thr Ser Pro Ser Thr Ala Ala Thr Ala Ile Thr Pro Ala Leu Ile
20 25 30
Phe Pro Lys Ile Glu Pro Lys Leu Glu Pro Phe Asp Ala Pro Thr Leu
35 40 45
Pro Leu Gln Ser Phe Pro Gln Asn Pro Ser Pro Asn Phe Phe Val Asn
50 55 60
Ser Gly Gln Leu Pro Phe Val Gly Pro Gly Ser Asn Pro Asn Asp Thr
65 70 75 80
Val Phe Ser Gln Thr Pro Glu Gly Ser Pro Glu Glu Asn Asn Val Tyr
85 90 95
Ser Glu Tyr Tyr Arg Ile Ser Glu Leu Phe Arg Thr Ala Phe Ser Lys
100 105 110
Arg Met Glu Asn Leu Gly Asn Ile Glu Val Leu Asp Pro Asp Ser Arg
115 120 125
Ala Ile Val Pro Val Pro Glu Glu Thr Arg Ile Ser Asn Val Val Val
130 135 140
Ser Arg Arg Arg Asp Gln Arg Ser Ser Glu Leu Val Arg Val Thr Asp
145 150 155 160
Leu Thr Ile Asp His Val Arg Tyr Phe Arg Asp Leu Val Arg Arg Thr
165 170 175
Arg Met Leu Tyr Asp Ala Leu Arg Ile Phe Ser Met Met Glu Glu Glu
180 185 190
Lys Arg Arg Glu Val Gly Leu Ile Thr Arg Arg Ser Arg Gly Asp Leu
195 200 205
Arg Ala Ala Lys Leu Met Lys Asp Arg Gly Leu Trp Leu Asn Arg Asp
210 215 220
Lys Arg Ile Val Gly Ser Ile Pro Gly Ile Asn Ile Gly Asp Leu Phe
225 230 235 240
Leu Phe Arg Met Glu Leu Cys Val Val Gly Leu His Gly Gln Ala Gln
245 250 255
Ala Gly Ile Asp Tyr Leu Pro Gly Ser Arg Ser Ser Asn Gly Glu Pro
260 265 270
Ile Ala Thr Ser Ile Ile Val Ser Gly Gly Tyr Glu Asp Asp Gln Asp
275 280 285
Glu Gly Asp Val Leu Ile Tyr Thr Gly His Gly Gly Gln Asp Lys Phe
290 295 300
Ser Arg Gln Cys Asp His Gln Lys Leu Glu Gly Gly Asn Leu Ala Leu
305 310 315 320
Glu Arg Ser Met His Tyr Gly Ile Glu Val Arg Val Ile Arg Gly Ile
325 330 335
Lys Tyr Glu Gly Ser Val Thr Gly Lys Val Tyr Val Tyr Asp Gly Leu
340 345 350
Tyr Lys Ile His Asp Ser Trp Phe Asp Val Gly Lys Ser Gly Phe Gly
355 360 365
Val Tyr Lys Tyr Lys Leu Leu Arg Asn Glu Gly Gln Ala Glu Met Gly
370 375 380
Ser Ala Ile Leu Arg Phe Ala Glu Asn Leu Arg Val Ser Pro Leu Thr
385 390 395 400
Val Arg Pro Val Gly Tyr Leu Cys Asp Asp Leu Ser Thr Lys Lys Glu
405 410 415
Asn Ile Pro Val Phe Leu Phe Asn Asp Ile Asp Gly Asp Asn Glu Pro
420 425 430
Met Tyr Tyr Glu Tyr Leu Pro Arg Thr Val Phe Pro Leu His Ala Tyr
435 440 445
Asn Leu Gly Gly Asn Gly Ser Gly Cys Asp Cys Val Ala Gly Cys Thr
450 455 460
Asp Asp Cys Val Cys Ala Gln Arg Asn Gly Gly Glu Phe Ala Tyr Asp
465 470 475 480
Gln Asn Gly Phe Leu Leu Arg Gly Lys Pro Val Ile Phe Glu Cys Gly
485 490 495
Ser Phe Cys Arg Cys Pro Pro Thr Cys Arg Asn Arg Leu Thr Gln Lys
500 505 510
Gly Leu Arg Asn Arg Phe Glu Val Phe Arg Ser Arg Glu Thr Gly Trp
515 520 525
Gly Val Arg Ser Leu Asp Leu Ile Gln Ala Gly Ala Phe Ile Cys Glu
530 535 540
Tyr Ala Gly Val Val Leu Thr Arg Glu Gln Ala Ala Leu Phe Ser Met
545 550 555 560
Asn Gly Asp Thr Leu Ile Tyr Pro Asn Arg Phe Thr Asp Arg Trp Ala
565 570 575
Glu Trp Gly Asp Phe Ser Lys Val Tyr Ser Asp Tyr Val Arg Pro Met
580 585 590
His Pro Ser Ile Pro Pro Leu Asp Phe Ala Met Asp Val Ser Arg Met
595 600 605
Arg Asn Leu Ala Cys Tyr Met Ser His Ser Ser Cys Pro Asn Val Leu
610 615 620
Val Gln Phe Val Leu Tyr Asp His His Asn Leu Leu Phe Pro Arg Leu
625 630 635 640
Met Leu Phe Ala Met Glu Asn Ile Pro Pro Leu Arg Glu Leu Ser Leu
645 650 655
Asp Tyr Gly Val Ala Asp Glu Trp Thr Gly Lys Leu Pro Ile Cys Asn
660 665 670
<210> 38
<211> 695
<212> PRT
<213> 黄瓜
<400> 38
Met Asp Ser Pro Ile Pro Phe Gln Asp Leu Asn Leu Leu Pro Asp Pro
1 5 10 15
Ser Ser Thr Ala Val Met Thr Ala Ala Thr Ser Pro Lys Thr Ala Thr
20 25 30
Gly Ile Asn Ser Ser Phe Asn Lys Phe Val Asp Thr Gly Lys Leu Leu
35 40 45
Thr Pro Lys Leu Glu Pro Lys Leu Glu Pro Phe Asp Asp Leu Phe Glu
50 55 60
Thr Arg Glu Ser Gln Gln Pro Gln Ser Val Gln Gln Pro Phe Leu Ser
65 70 75 80
Thr Pro Ser Ser Asn Phe Phe Ser Asn Thr Asp Phe Ser Gln Thr Pro
85 90 95
Phe Ser Asp Gln Asn His Thr Pro Leu Ser Gln Ser Ser Ser Ile Ser
100 105 110
Ser Asp Lys Asp Asn Val Tyr Ser Glu Phe Tyr Arg Ile Ser Gln Leu
115 120 125
Phe Arg Ser Ala Phe Gly Lys Gly Leu Gln Ser Tyr Gly Asp Ala Asp
130 135 140
Val Glu Val Val Asp Pro Asp Ala Gln Ala Ile Val Pro Val Pro Glu
145 150 155 160
Glu Asn Gln Ile Ser Thr Val Val Val Ser Lys Arg Arg Tyr Asp Lys
165 170 175
Arg Ser Ser Glu Leu Val Arg Val Thr Asp Leu Gly Val Glu Asp Gln
180 185 190
Arg Tyr Phe Arg Asp Val Val Arg Arg Thr Arg Met Ile Phe Asp Ser
195 200 205
Leu Arg Val Leu Ser Thr Ala Glu Glu Glu Lys Ser Pro Gly Leu Met
210 215 220
Arg Arg Leu Arg Gly Asp Leu Arg Ala Ser Ser Leu Met Arg Glu Arg
225 230 235 240
Gly Leu Trp Leu Asn Arg Asp Lys Arg Ile Val Gly Ser Ile Pro Gly
245 250 255
Val His Ile Gly Asp Leu Phe Phe Phe Arg Met Glu Leu Cys Val Val
260 265 270
Gly Leu His Gly Gln Ala Gln Ala Gly Ile Asp Tyr Val Pro Ala Ser
275 280 285
Gln Ser Ser Asn Gly Glu Pro Ile Ala Thr Ser Ile Ile Val Ser Gly
290 295 300
Gly Tyr Glu Asp Asp Glu Asp Ala Gly Asp Met Ile Ile Tyr Thr Gly
305 310 315 320
His Gly Gly Gln Asp Lys Phe Ser Lys Gln Cys Met His Gln Lys Leu
325 330 335
Glu Gly Gly Asn Leu Ala Leu Glu Arg Ser Met His Tyr Gly Ile Glu
340 345 350
Val Arg Val Ile Arg Gly Met Lys Tyr Ala Gly Ser Val Ala Ser Lys
355 360 365
Ile Tyr Val Tyr Asp Gly Leu Tyr Arg Ile Leu Asp Cys Trp Phe Asp
370 375 380
Val Gly Lys Ser Gly Phe Gly Val Tyr Lys Tyr Lys Leu Leu Arg Ile
385 390 395 400
Asp Gly Gln Ala Glu Met Gly Ser Ser Ile Leu Lys Phe Ala Glu Asn
405 410 415
Leu Arg Thr Lys Pro Leu Ser Leu Arg Pro Ser Gly Tyr Leu Ser Leu
420 425 430
Asp Ile Ser Met Lys Lys Glu Ala Val Pro Val Leu Leu Phe Asn Asp
435 440 445
Ile Asp Asn Asp Gln Glu Pro Leu Tyr Tyr Glu Tyr Leu Val Arg Thr
450 455 460
Val Phe Pro Pro Phe Ala Phe His Gln Ser Gly Ser Gly Thr Gly Cys
465 470 475 480
Ser Cys Val Thr Ser Cys Val His Asp Cys Phe Cys Ala Met Lys Asn
485 490 495
Gly Gly Glu Phe Gly Tyr Asp Gln Asn Gly Phe Leu Val Arg Gly Lys
500 505 510
Pro Ile Ile Phe Glu Cys Gly Pro Phe Cys Gln Cys Pro Pro Gln Cys
515 520 525
Arg Asn Arg Val Ser Gln Lys Gly Leu Lys His Arg Leu Glu Val Phe
530 535 540
Arg Ser Arg Glu Thr Gly Trp Gly Val Arg Ser Leu Asp Leu Ile His
545 550 555 560
Ala Gly Ala Phe Ile Cys Glu Tyr Ala Gly Val Val Leu Thr Arg Glu
565 570 575
Gln Ala Gln Val Phe Ser Met Asn Gly Asp Thr Leu Ile Tyr Pro Asn
580 585 590
Arg Phe Ser Asp Arg Trp Ala Glu Trp Gly Asp Leu Ser Gln Ile Tyr
595 600 605
Ser Asn Tyr Val Arg Pro Ser Tyr Pro Ser Val Pro Pro Leu Asp Phe
610 615 620
Ala Met Asp Val Ser Arg Met Arg Asn Val Ala Cys Tyr Ile Ser His
625 630 635 640
Ser Thr Ser Pro Asn Val Leu Val Gln Phe Val Leu Tyr Asp His Asn
645 650 655
Asn Leu Met Phe Pro His Leu Met Leu Phe Ala Met Glu Asn Ile Pro
660 665 670
Pro Leu Arg Glu Leu Ser Ile Asp Tyr Gly Val Ala Asp Asp Trp Ser
675 680 685
Gly Lys Leu Ala Ile Cys Asn
690 695
<210> 39
<211> 654
<212> PRT
<213> 琴叶拟南芥
<400> 39
Met Ser Thr Val Val Pro Phe Pro Asp Leu Asn Leu Met Pro Ala Ser
1 5 10 15
Gln Ser Ser Thr Ala Gly Thr Thr Ala Gly Asp Thr Val Val Thr Gly
20 25 30
Lys Leu Glu Val Lys Ala Glu Pro Val Glu Glu Cys Gln Thr Pro Pro
35 40 45
Ser Ser Phe Ser Asp Gln Ser Ala Ser Thr Asp Leu Ile Ala Glu Phe
50 55 60
Ile Arg Ile Ser Lys Leu Phe Arg Ser Ala Phe Lys Pro His Gln Ile
65 70 75 80
Glu Gly Leu Asp Gly Val Ser Val Tyr Gly Leu Asp Ser Gly Ala Ile
85 90 95
Val Thr Val Pro Glu Glu Glu Asn Arg Glu Leu Ile Lys Pro Pro Pro
100 105 110
Gly Phe Gly Asp Asn Arg Val Ser Thr Val Val Val Ser Pro Pro Pro
115 120 125
Thr Lys Phe Glu Arg Pro Arg Glu Leu Ala Arg Ile Ala Ile Leu Gly
130 135 140
His Asp Gln Arg Lys Glu Leu Arg Gln Val Met Lys Arg Thr Arg Met
145 150 155 160
Thr Tyr Glu Ser Leu Arg Ile His Leu Met Ala Glu Ser Met Lys Asn
165 170 175
Pro Val Leu Gly Gln Gly Arg Arg Arg Arg Ser Asp Met Ala Ala Ala
180 185 190
Tyr Ile Met Arg Asp Arg Gly Leu Trp Leu Asn Tyr Asp Lys His Ile
195 200 205
Val Gly Pro Ile Ser Gly Val Glu Ile Gly Asp Ile Phe Phe Tyr Arg
210 215 220
Met Glu Leu Cys Val Val Gly Leu His Gly Gln Thr Gln Ala Gly Ile
225 230 235 240
Asp Cys Leu Thr Ala Glu Arg Ser Ala Thr Gly Glu Pro Ile Ala Thr
245 250 255
Ser Ile Val Val Ser Gly Gly Tyr Glu Asp Asp Glu Asp Thr Gly Asp
260 265 270
Val Leu Val Tyr Thr Gly His Gly Gly Gln Asp Lys Gln His Lys Gln
275 280 285
Cys Asp Asn Gln Arg Leu Val Gly Gly Asn Leu Gly Met Glu Arg Ser
290 295 300
Met His Tyr Gly Ile Glu Val Arg Val Ile Arg Gly Ile Lys Tyr Glu
305 310 315 320
Asn Ser Ile Ser Ser Lys Val Tyr Val Tyr Asp Gly Leu Tyr Lys Ile
325 330 335
Val Asp Cys Trp Phe Ala Val Gly Lys Ser Gly Phe Gly Val Phe Lys
340 345 350
Phe Arg Leu Val Arg Met Asp Gly Gln Pro Met Met Gly Ser Ala Val
355 360 365
Met Arg Phe Ala Gln Thr Leu Arg Ser Lys Pro Leu Met Val Arg Pro
370 375 380
Thr Gly Tyr Val Ser Phe Asp Leu Ser Asn Lys Lys Glu Asn Val Pro
385 390 395 400
Val Phe Leu Tyr Asn Asp Val Asp Gly Asp Gln Glu Pro Arg His Tyr
405 410 415
Glu Tyr Ile Ala Lys Ser Val Phe Pro Pro Gly Ile Phe Gly Gln Gly
420 425 430
Gly Ile Ser Arg Thr Gly Cys Asp Cys Lys Leu Ser Cys Thr Asp Asp
435 440 445
Cys Leu Cys Ala Arg Lys Asn Gly Gly Glu Phe Ala Tyr Asp Asp Asn
450 455 460
Gly His Leu Leu Arg Gly Lys Asp Val Val Phe Glu Cys Gly Glu Leu
465 470 475 480
Cys Thr Cys Gly Pro Asn Cys Lys Ser Arg Val Thr Gln Lys Gly Leu
485 490 495
Arg Asn Arg Leu Glu Val Phe Arg Ser Lys Glu Thr Gly Trp Gly Val
500 505 510
Arg Thr Leu Asp Leu Ile Glu Ala Gly Ala Phe Ile Cys Glu Tyr Ala
515 520 525
Gly Val Val Val Thr Arg His Gln Ala Glu Ile Leu Ser Met Asn Gly
530 535 540
Asp Val Met Val Tyr Pro Gly Arg Phe Thr Asp Lys Trp Arg Asn Trp
545 550 555 560
Gly Asp Leu Ser Gln Val Tyr Pro Asp Ser Val Arg Pro Asp Tyr Pro
565 570 575
Ser Leu Pro Pro Leu Asp Phe Ala Met Asp Val Ser Arg Met Arg Asn
580 585 590
Val Ala Cys Tyr Ile Ser His Ser Lys Asp Pro Asn Val Met Val Gln
595 600 605
Phe Val Leu Tyr Asp His Asn His Leu Met Phe Pro Arg Val Met Leu
610 615 620
Phe Ala Leu Glu Asn Ile Ser Pro Leu Ala Glu Leu Ser Leu Asp Tyr
625 630 635 640
Gly Leu Ala Asp Glu Val Asn Gly Lys Leu Ala Ile Cys Asn
645 650
<210> 40
<211> 349
<212> PRT
<213> 拟南芥
<400> 40
Met Gly Arg Pro Pro Ser Asn Gly Gly Pro Ala Phe Arg Phe Ile Leu
1 5 10 15
Pro Glu Val Thr Glu Met Glu Ala Ile Leu Leu Gln His Asn Thr Ala
20 25 30
Met Pro Gly Arg His Ile Leu Glu Ala Leu Ala Asp Lys Phe Ser Glu
35 40 45
Ser Pro Glu Arg Lys Gly Lys Val Val Val Gln Phe Lys Gln Ile Trp
50 55 60
Asn Trp Phe Gln Asn Arg Arg Tyr Ala Leu Arg Ala Arg Gly Asn Lys
65 70 75 80
Ala Pro Gly Lys Leu Asn Val Ser Ser Met Pro Arg Met Asp Leu Pro
85 90 95
Asn Gln Met Arg Ser Val Ile Gln Pro Leu Ser Val Pro Lys Thr Thr
100 105 110
His Met Thr Gly Asn Leu Pro Gly Met Thr Pro Ala Pro Ser Gly Ser
115 120 125
Leu Val Pro Gly Val Met Arg Ser Gly Ser Asp Asn Ser Tyr Leu Glu
130 135 140
Phe Glu Ala Lys Ser Ala Arg Asp Gly Ala Trp Tyr Asp Val Gln Ala
145 150 155 160
Phe Leu Ala His Arg Asn Leu Glu Ile Gly Asp Pro Glu Val Gln Val
165 170 175
Arg Phe Ala Gly Phe Glu Val Glu Glu Asp Glu Trp Ile Asn Val Lys
180 185 190
Lys His Val Arg Gln Arg Ser Leu Pro Cys Glu Ala Ser Glu Cys Val
195 200 205
Ala Val Leu Ala Gly Asp Leu Val Leu Cys Phe Gln Glu Gly Lys Asp
210 215 220
Gln Ala Leu Tyr Phe Asp Ala Ile Val Leu Asp Ala Gln Arg Arg Arg
225 230 235 240
His Asp Val Arg Gly Cys Arg Cys Arg Phe Leu Val Arg Tyr Ser His
245 250 255
Asp Gln Ser Glu Gln Glu Ile Val Pro Leu Arg Lys Ile Cys Arg Arg
260 265 270
Pro Glu Thr Asp Tyr Arg Leu Gln Gln Leu His Asn Ala Val Asn Asp
275 280 285
Leu Ala Asn Ser Asn Gln His Gln Ile Pro Ala Leu Asp Ala Ala Ala
290 295 300
Lys Thr Pro Leu Ser Leu Pro Gly Ala Thr Val Pro Ile Val Ala Pro
305 310 315 320
Glu Ser Lys Asp Pro Ser Leu Ser Ala Thr Pro Ala Thr Leu Val Gln
325 330 335
Pro Ser Ser Asn Ala Ala Thr Val Pro Ala Gly Ser Ala
340 345
<210> 41
<211> 420
<212> PRT
<213> 拟南芥
<400> 41
Met Tyr Pro Thr Gly Gln Gln Ile Ser Phe Gln Thr Thr Pro Leu Asn
1 5 10 15
Val Gln Asp Pro Thr Arg Met Met Asn Leu Asp Gln Ser Ser Pro Val
20 25 30
Ala Arg Asn Glu Thr Gln Asn Gly Gly Gly Ile Ala His Ala Glu Phe
35 40 45
Ala Met Phe Asn Ser Lys Arg Leu Glu Ser Asp Leu Glu Ala Met Gly
50 55 60
Asn Lys Ile Lys Gln His Glu Asp Asn Leu Lys Phe Leu Lys Ser Gln
65 70 75 80
Lys Asn Lys Met Asp Glu Ala Ile Val Asp Leu Gln Val His Met Ser
85 90 95
Lys Leu Asn Ser Ser Pro Thr Pro Arg Ser Glu Asn Ser Asp Asn Ser
100 105 110
Leu Gln Gly Glu Asp Ile Asn Ala Gln Ile Leu Arg His Glu Asn Ser
115 120 125
Ala Ala Gly Val Leu Ser Leu Val Glu Thr Leu His Gly Ala Gln Ala
130 135 140
Ser Gln Leu Met Leu Thr Lys Gly Val Val Gly Val Val Ala Lys Leu
145 150 155 160
Gly Lys Val Asn Asp Glu Asn Leu Ser Gln Ile Leu Ser Asn Tyr Leu
165 170 175
Gly Thr Arg Ser Met Leu Ala Val Val Cys Arg Asn Tyr Glu Ser Val
180 185 190
Thr Ala Leu Glu Ala Tyr Asp Asn His Gly Asn Ile Asp Ile Asn Ala
195 200 205
Gly Leu His Cys Leu Gly Ser Ser Ile Gly Arg Glu Ile Gly Asp Ser
210 215 220
Phe Asp Ala Ile Cys Leu Glu Asn Leu Arg Pro Tyr Val Gly Gln His
225 230 235 240
Ile Ala Asp Asp Leu Gln Arg Arg Leu Asp Leu Leu Lys Pro Lys Leu
245 250 255
Pro Asn Gly Glu Cys Pro Pro Gly Phe Leu Gly Phe Ala Val Asn Met
260 265 270
Ile Gln Ile Asp Pro Ala Tyr Leu Leu Cys Val Thr Ser Tyr Gly Tyr
275 280 285
Gly Leu Arg Glu Thr Leu Phe Tyr Asn Leu Phe Ser Arg Leu Gln Val
290 295 300
Tyr Lys Thr Arg Ala Asp Met Ile Ser Ala Leu Pro Cys Ile Ser Asp
305 310 315 320
Gly Ala Val Ser Leu Asp Gly Gly Ile Ile Arg Lys Thr Gly Ile Phe
325 330 335
Asn Leu Gly Asn Arg Asp Glu Val Asn Val Arg Phe Ala Lys Pro Thr
340 345 350
Ala Ser Arg Thr Met Asp Asn Tyr Ser Glu Ala Glu Lys Lys Met Lys
355 360 365
Glu Leu Lys Trp Lys Lys Glu Lys Thr Leu Glu Asp Ile Lys Arg Glu
370 375 380
Gln Val Leu Arg Glu His Ala Val Phe Asn Phe Gly Lys Lys Lys Glu
385 390 395 400
Glu Phe Val Arg Cys Leu Ala Gln Ser Ser Cys Thr Asn Gln Pro Met
405 410 415
Asn Thr Pro Arg
420
<210> 42
<211> 163
<212> PRT
<213> 番茄(Solanum lycopersicum)
<400> 42
Met Lys Asp Val Ile Gly Ile Val Ala Leu Leu Gly Lys Val Asp Asp
1 5 10 15
Asp Asn Leu Ser Arg Lys Gly Pro Met Asn Lys Ser Ser Gly Leu His
20 25 30
Gly Val Gly Ala Ser Ile Gly Arg Pro Leu Asp Asp Arg Tyr Leu Val
35 40 45
Ile Phe Leu Glu Asn Leu Arg Pro Tyr Ala Gly Glu Phe Ile Val Asp
50 55 60
Asp Pro Gln Arg Arg Leu Ala Ile Arg Arg Lys Pro Arg Tyr Val Asn
65 70 75 80
Glu Glu Thr Pro His Val Phe Leu Gly Phe Ala Val Asn Met Ile Asn
85 90 95
Ile Asp Thr Ala Asn Leu Tyr Cys Val Thr Arg Thr Gly Tyr Gly Leu
100 105 110
Arg Glu Thr Leu Leu Tyr Gly Leu Phe Ser Gln Leu Gln Val Tyr Lys
115 120 125
Thr Ser Ala Asp Met Met Glu Ala Leu Pro Phe Ile Ile Asp Gly Ala
130 135 140
Ile Ser Leu Asp Gly Gly Ile Ile Lys Ser Gly Gly Ile Phe Ser Leu
145 150 155 160
Gly Lys Arg
<210> 43
<211> 1790
<212> PRT
<213> 马铃薯(Solanum tuberosum)
<400> 43
Met Ser Ser Lys Arg Gln Cys Asp Asp Phe Ser Ala Glu Thr Pro Arg
1 5 10 15
Lys Lys Pro Ser Arg Val Leu Arg Ile Gln Val Asp Ser Asp Glu Glu
20 25 30
Val Gly Thr Asn Glu Gly Arg Val Phe Tyr Phe Arg Val Leu Leu Pro
35 40 45
Asn Gly Ile Thr Leu Glu Leu Gln Val Pro Gly Pro Pro Ser Glu Met
50 55 60
Pro Val Glu Asp Phe Val Ile Leu Val Arg Arg Glu Tyr Gln Asn Leu
65 70 75 80
Gly Arg Arg Thr Glu Ser Pro Lys Pro Lys Arg Gln Ile Asn Trp Thr
85 90 95
Ser Lys Asp Leu His Phe Val Asp Ala Phe Glu Asn Arg Ile Thr Lys
100 105 110
Met Leu Asp Phe Arg Lys Phe Lys Ser Asn Lys Ser His Met Ile Arg
115 120 125
Leu Cys Asp Gly Ser Ala Glu Ala Asp Lys Tyr Glu Asn Met Trp Asp
130 135 140
Leu Thr Pro Asp Thr Asp Leu Leu Lys Glu Leu Pro Glu Glu Tyr Thr
145 150 155 160
Phe Glu Thr Ala Leu Ala Asp Leu Ile Asp Asn Ser Leu Gln Ala Val
165 170 175
Trp Pro Lys Ser Thr Asp Gln Arg Arg Leu Ile Ser Leu Glu Leu Thr
180 185 190
Lys Ser Arg Ile Thr Ile Phe Asp Thr Gly Leu Gly Met Asp Gly Ser
195 200 205
Ala Glu Asn Ser Ile Val Lys Trp Gly Lys Met Gly Ala Ser Leu His
210 215 220
Arg Leu Ser Arg Asp Arg Gly Ile Gly Gly Lys Pro Pro Tyr Leu Thr
225 230 235 240
Pro Tyr Phe Gly Met Phe Gly Tyr Gly Gly Pro Ile Ala Ser Met His
245 250 255
Leu Gly Arg Arg Ala Ser Val Ser Ser Lys Thr Lys Glu Cys Lys Lys
260 265 270
Val Phe Val Leu His Leu Glu Arg Asp Ser Leu Leu Arg Cys Ser Ser
275 280 285
Ser Gln Gln Thr Trp Arg Thr Asp Gly Asn Val Arg Asp Pro Leu Glu
290 295 300
Asp Glu Leu Arg Tyr Ser Val Asp Gly Ser Phe Thr Lys Val Glu Ile
305 310 315 320
Phe Tyr Pro Lys Met Arg Ser Glu Ser Val Gln Lys Leu Gln Tyr Lys
325 330 335
Leu Lys Asp Ile Tyr Phe Pro Tyr Ile Gln Cys Asp Glu Val Ser Lys
340 345 350
Thr Gly Lys Thr Val Met Pro Ile Glu Phe Gln Val Asn Gly Thr Asn
355 360 365
Leu Ala Glu Ile Glu Gly Gly Glu Val Ala Thr Thr Asn Leu Leu Ser
370 375 380
Cys Asn Gly Pro Glu Phe Val Met Gln Leu Ser Phe His Val Lys Asp
385 390 395 400
Ser Asn Ser Leu Lys Ile Gly Ser Gly Thr Lys Ser Ser Phe Glu Gly
405 410 415
His Ala Arg Leu Arg Cys Val Tyr Phe Pro Met Val Gln Gly Lys Glu
420 425 430
Ser Ile Glu Val Ile Leu Glu Lys Leu Glu Ala Asp Gly Tyr Gly Ile
435 440 445
Thr Glu Asn Phe Glu Thr Phe Ser His Val Ser Val Arg Arg Leu Gly
450 455 460
Arg Leu Leu Pro Asp Ala Arg Trp Ser Trp Leu Pro Phe Met Glu Pro
465 470 475 480
Lys Leu Arg Lys Ser Asp Arg Ala Glu Val Leu Lys Arg Cys Cys Phe
485 490 495
Arg Val Lys Cys Phe Ile Glu Thr Asp Ala Gly Phe Asn Pro Thr Pro
500 505 510
Ser Lys Thr Asp Leu Ala His His His Pro Cys Thr Ile Ala Leu Arg
515 520 525
Asn Phe Gly Asn Lys Pro Ser Asp Lys Glu Asn Asp Val Leu Ile Glu
530 535 540
Ile Ser Lys Asp Gly Lys Lys Leu Ser Leu Leu Gln Leu Glu Lys Leu
545 550 555 560
Tyr Gln Asp Trp Leu Leu Gln Met His Asp Arg Tyr Asp Glu Glu Ile
565 570 575
Asp Cys Gly Glu Asp Gln Pro Thr Phe Val Ile Gly Pro Ser His Lys
580 585 590
Lys Glu Leu Gly Val Ser Ala Asp Val Leu Arg Ile His Lys Ala Phe
595 600 605
Gln Arg Lys Gly Ile Thr Trp Lys Ala Gly Gln Lys Ile Lys Ile Leu
610 615 620
Lys Gly Ala Cys Arg Gly Phe His Lys Asn Asn Ile Phe Ala Thr Leu
625 630 635 640
Glu Phe Ile Ile Leu Glu Gly Trp Gln Gly Asp Ser Gly Gly Glu Ala
645 650 655
Arg Ile Ile Cys Arg Pro Leu His Val Pro Ala Glu Ser Gly Cys Arg
660 665 670
Leu Thr Phe Asp Glu Gly Cys Ala Cys Phe Glu Ile Arg Asp Ser Lys
675 680 685
Ser Phe Pro Ile Ser Val Ile Asp Ala Gly Lys Cys Leu Ala Val Asp
690 695 700
Asn Thr Glu Trp Glu Asn Gln Ile Leu Lys His Gln Glu Lys Thr Thr
705 710 715 720
Pro Ser Ser Ile Asp Ile Leu Asp Ala Glu Gln Cys Gln Glu Leu Asp
725 730 735
Ile Lys Gly Ala Leu Pro Gln Asp Val Asp Ala Gly His Glu Pro Pro
740 745 750
Glu Glu Ile Thr Ala Val Val Arg Pro Ala Ser Phe Ser Ser Val Thr
755 760 765
Ala Tyr Lys Asn Leu Asp Gln Lys Tyr Ile Met Lys Glu Asn Phe Glu
770 775 780
Met Thr Leu Glu Ile Lys Phe Lys Ala Asp Glu Asn Glu Lys Glu Arg
785 790 795 800
His Ile Tyr Ser Gly Gln Leu Asn Pro Ser Ser Leu Lys Gly Phe His
805 810 815
Gly Leu Tyr Ile Phe Pro Leu Lys Lys Lys Ser Pro Asn Leu Phe Gln
820 825 830
Lys Ala Gly Ile Tyr Leu Phe Arg Phe Ser Leu Ile Glu Ser Arg Thr
835 840 845
Ile Ser Val Lys Glu Val Arg Val Lys Ala Leu Pro Glu Ala Ala Ser
850 855 860
Trp Glu Leu Val Ser Asp Gly Lys Ser Thr His Ser Val Arg Val Gly
865 870 875 880
Ser Cys Phe Pro Glu Val Phe Ser Val Ala Cys Cys Asp Arg Phe Cys
885 890 895
Asn Arg Ile Pro Phe Lys Ser Gln Thr Glu Ile Glu Met Lys Leu Cys
900 905 910
Ser Gly Gly Arg Ala Ile Ser Ser Glu Cys Ser Tyr Asp Gln Cys Ile
915 920 925
Thr His Asp Arg Tyr Thr Met Lys Phe Lys Asn Val Thr Ile Glu Ser
930 935 940
Ser Glu Leu Asp Met Ile Arg Pro Ser Tyr Lys Ala Thr Leu His Ile
945 950 955 960
Asn Ser Lys Glu Asp Pro Phe Phe Val Ala Ile Pro Cys Ala Val Ile
965 970 975
Pro Gly Pro Leu Gln Arg Ile Leu Leu Arg Pro Val Asp Phe Gly Lys
980 985 990
Lys Leu Val Pro Gly Met Val Leu Lys Glu Leu Ala Leu Glu Thr Phe
995 1000 1005
Asp Lys Tyr Gly Asn His Met Arg Lys Asp Glu His Ile Lys Leu Thr
1010 1015 1020
Leu Glu Gly Leu His Leu Leu Asp Lys Gly Asp Ser Phe Tyr Lys Val
1025 1030 1035 1040
Asp Asp His Gly Cys Val Asn Leu Ser Gly Thr Leu Lys Val Thr Ala
1045 1050 1055
Gly Tyr Gly Lys Leu Val Ser Leu Ser Val Leu Ser Gly Asp Lys Val
1060 1065 1070
Val Phe Lys Lys Glu Phe Gln Thr Asp Arg Arg Ser Leu Arg Val Ala
1075 1080 1085
Ser Lys Val Pro Lys Val Cys Ala Ala Gly Ser His Leu Glu Asp Val
1090 1095 1100
Val Phe Glu Val Ile Asn Ser Ala Gly Glu Val Asp Glu Asp Ile Asp
1105 1110 1115 1120
Ser Glu Val Glu Asp Gly His Ser His Thr Leu Leu Ile Arg Gln Asp
1125 1130 1135
Ser Leu Arg Glu Glu Asp Asn Val Arg Tyr Ser Phe His His Gly Arg
1140 1145 1150
Cys Ile Val Arg Ser Ile Pro Leu Pro Asp Asn Glu Gly Leu Phe Cys
1155 1160 1165
Phe Val Ala Ser His Ser Arg Phe His Glu Leu Gln Thr Ser Ile Glu
1170 1175 1180
Val His Val Glu Lys Ala Val Ile Arg Thr His Glu Leu Thr Gln Pro
1185 1190 1195 1200
Arg Ser Pro Lys Lys Glu Ile Leu Leu His Glu Asp Ser Asn Gly Lys
1205 1210 1215
Gly Pro Glu Thr Val Cys His Asn Thr Phe Asp Gly Arg Ile Met Ile
1220 1225 1230
Phe Asn Asp Ser Cys Ala Ser Met Asp Leu Glu Asp Arg Leu Gln Thr
1235 1240 1245
Val Thr Lys Leu Glu Cys Val Ser Arg Gln Lys Leu Gly Asp Asp Ile
1250 1255 1260
Cys Arg Tyr Gly Leu Cys Ile Arg Arg Cys Asp Ala Asn Val Glu Ser
1265 1270 1275 1280
Leu Asn Ile Lys Gln Ser Asn Ile Glu Leu Glu Met Ser Asn Leu Gly
1285 1290 1295
Val Val Trp Leu Tyr Val Val Arg Lys Leu Phe Asn Trp His Met Lys
1300 1305 1310
Ser Lys Ser Asn Val Met Cys Ile Met Asn His Ala Tyr Val Lys Pro
1315 1320 1325
Thr Ala Trp His Gly His Met Gln Lys Ala Val Val Thr Ser Ser Leu
1330 1335 1340
Asn His Asp Leu Glu Pro Cys Ser Phe Lys Pro Lys Asp Tyr Tyr Leu
1345 1350 1355 1360
Arg Arg Leu His Leu Leu Tyr Ile Leu Leu Tyr Leu Leu Ala Phe Arg
1365 1370 1375
Leu Arg Ser Val Asn Val Ser Glu Arg Ser Glu Pro Lys Ser Arg Asn
1380 1385 1390
Ile Asp Cys Trp His Gln Val His Val Val Ile Ser Leu Phe Gly Trp
1395 1400 1405
Phe Lys Cys Ser Pro Asp Ile Ser Phe Leu Phe Leu Met Ser Leu Glu
1410 1415 1420
Phe Ser Leu Asn Phe Ser Leu Leu Ser Val Pro Gly Ala Ile Thr Ser
1425 1430 1435 1440
Leu Ala Ile Ser Gln Ser Val Lys Leu Lys Glu Ile Ile Asn Cys Asn
1445 1450 1455
Met Lys Leu Val Met Asp Ala Ala Phe Ile Gly Leu Asp Ser Phe His
1460 1465 1470
Asp Leu Gly Tyr Asp Lys Asp Val Ile Met Glu Lys Ile Glu Gly Lys
1475 1480 1485
Ala Asp Ser Ala Ala Ala Val Ile His Lys Leu Leu Arg Ser Pro Lys
1490 1495 1500
Pro Glu Gln Leu Tyr Leu Lys Tyr Ala His Asp Ile Leu Gly Val Val
1505 1510 1515 1520
Ala Leu Leu Gly Glu Val Arg Thr His Lys Leu Ser Ser Met Phe Ser
1525 1530 1535
Thr Tyr Leu Gly Glu Asp Gln Met Leu Ala Val Val Cys Lys Ser Arg
1540 1545 1550
Ala Ala Ala Arg Ala Leu Glu Asn Tyr Arg Met Asp Gly Asn Val Asn
1555 1560 1565
Cys Ala Ser Ala Leu Asp Ile Leu Ala Ala Lys Leu Gly Ile Ser Ile
1570 1575 1580
Lys Gly Arg Tyr Leu Val Ile Cys Leu Glu Asp Ile Arg Pro Tyr Lys
1585 1590 1595 1600
Gln Gly Val Ser Ser Asp Pro Gln Arg Glu Leu Ala Ile Pro His Pro
1605 1610 1615
Thr Leu Ser Asn Arg Glu Thr Pro Pro Gly Phe Leu Gly Tyr Ala Val
1620 1625 1630
Asn Met Ile Phe Leu Pro Ala Glu Tyr Leu Gln Leu Arg Thr Ala Ser
1635 1640 1645
Gly His Gly Leu Arg Glu Thr Leu Phe Tyr Arg Leu Phe Gly Lys Leu
1650 1655 1660
Gln Val Tyr Lys Ser Arg Glu Gln Leu Tyr Met Ala Ser Ser Cys Ile
1665 1670 1675 1680
Glu Glu Gly Ala Val Ser Leu Asp Gly Gly Met Met Arg Gly Asn Gly
1685 1690 1695
Val Val Ser Ala Ser Val Gly Ser Glu Glu Pro Tyr Ile Leu Phe Pro
1700 1705 1710
Val Ile Cys Leu Glu Arg Gln Leu Leu Leu Ser Pro Glu Lys Val Lys
1715 1720 1725
Arg Leu Lys Arg Ile Glu Glu Leu Lys Leu Glu Arg Asn Gln Leu Gln
1730 1735 1740
Asp Gln Ile Gln Glu Glu Leu Arg Asn Glu Ala Lys Tyr Lys Lys Lys
1745 1750 1755 1760
Leu Ala Lys Lys Leu Arg Asp Lys Lys Gln Ile Asp Asp Gln Leu Glu
1765 1770 1775
Pro Ser Pro Gly Met Tyr Ser Ser Asp Met Asp Ile Ser Cys
1780 1785 1790
<210> 44
<211> 350
<212> PRT
<213> 菜豆(Phaseolus vulgaris)
<400> 44
Met Ser Gln Leu Asn His Thr Gln Ser Leu Arg Asp Asp Ile Ser Glu
1 5 10 15
Arg Ser Lys Ile Ile Lys Gln His Glu Asp Asn Leu Lys Phe Leu Asn
20 25 30
Ser Gln Ser Asn Gln Leu Ala Glu Ser Ile Phe Asp Leu Gln Val Ser
35 40 45
Leu Ala Lys Tyr His Ser Asn Asn Val Ile Thr Ser Asp Ser Gly Asn
50 55 60
Gly Ala Phe His Thr Glu Glu Glu Thr Met Glu Gln Ile Met Lys Lys
65 70 75 80
Glu Asn Thr Ala Ala Gly Val Phe Ser Trp Leu Lys Gly Asn Ala Gln
85 90 95
Thr Ser Asn Leu Thr Leu Thr Lys Asp Val Val Gly Val Val Ala Thr
100 105 110
Leu Gly Lys Val Glu Ser Asp Asn Leu Ser Arg Ile Leu Ser Glu Phe
115 120 125
Leu Gly Leu Glu Arg Met Leu Ala Ile Val Cys Ser Ser Tyr Glu Gly
130 135 140
Ile Asn Ala Leu Glu Lys Tyr Asp Thr Glu Gly Leu Ile Asn Cys Asn
145 150 155 160
Ala Gly Leu His Gly Ile Gly Ser Ser Ile Gly Lys Arg Ile Asn Gly
165 170 175
Arg Phe Ala Val Ile Ser Leu Glu Asp Leu Arg Pro Phe Val Gly Gly
180 185 190
Leu Val Ala Asp Asp Pro Gln Lys Lys Leu Ala Leu Pro Lys Pro Arg
195 200 205
Leu Pro Asn Gly Glu Cys Pro Pro Gly Phe Leu Asp Tyr Ala Val Asn
210 215 220
Met Ile His Leu Asp Ser Lys His Leu Ser Phe Leu Thr Asp Ile Gly
225 230 235 240
His Gly Leu Arg Glu Thr Leu Phe Tyr Gly Leu Phe Ser Arg Leu Gln
245 250 255
Ile Tyr Lys Thr Arg Asn Glu Met Leu Leu Ala Leu Pro Cys Ile Tyr
260 265 270
Asp Gly Ala Leu Ser Leu Asp Gly Gly Met Ile Gln Arg Gly Gly Met
275 280 285
Phe Ala Leu Gly Cys Arg Lys Asp Val Glu Val Lys Phe Pro Leu Ile
290 295 300
Ser Gly Gly Ser Asp Val Pro Ser Asn Tyr Ile Glu Thr Glu Glu Thr
305 310 315 320
Val Arg Lys Leu Asn Trp Glu Thr Ser Lys Leu Ser Ala Asp Lys His
325 330 335
Arg Glu Gln Gln Leu Leu Asp Tyr Arg Lys Gly Asn Leu His
340 345 350
<210> 45
<211> 431
<212> PRT
<213> 葡萄
<400> 45
Met Phe Gln Pro His Asn Gln Leu Ser Ile Gln Thr Lys Pro Leu Ala
1 5 10 15
Ile Gln Glu Pro Ser Ala Leu Met His Val Asp Pro Asn Asn Ser Phe
20 25 30
Gly Ala Ala Arg Asp Glu Met His Asn Gly Gly Leu Ser Lys Ala Glu
35 40 45
Ser Ala Val Asn Asn Ser Lys Lys Leu Gln Asp Glu Leu Gln Thr Leu
50 55 60
Gly Phe Lys Ile Lys Gln His Glu Asp Asn Ile Lys Tyr Leu Lys Thr
65 70 75 80
Gln Lys Asp Asn Leu Asp Gly Ser Ile Leu Asp Leu Gln Val Thr Leu
85 90 95
Gly Lys Tyr Cys Ser Ser Ser Ile Pro Thr Met Glu Asn Glu Ala Leu
100 105 110
Ser Lys Ser Arg Ser Glu Asn Glu Thr Val Glu Gln Ile Leu Lys Tyr
115 120 125
Glu Lys Ser Ala Ala Ala Ile Leu Cys Gln Leu Lys Ile Arg His Gly
130 135 140
Ser Gln Ala Ser His Leu Thr Leu Ala Lys Asp Val Leu Gly Ile Val
145 150 155 160
Ala Thr Leu Gly Lys Val Asp Asp Glu Asn Leu Ser Arg Leu Phe Ser
165 170 175
Glu Tyr Leu Gly Leu Glu Thr Met Met Ala Ile Val Cys Lys Thr Tyr
180 185 190
Glu Gly Val Lys Thr Leu Glu Thr Tyr Asp Phe Glu Gly Gly Ile Asn
195 200 205
Lys His Ser Gly Leu His Gly Leu Gly Ser Ser Ile Gly Arg His Leu
210 215 220
Asp Gly Arg Phe Arg Ile Ile Cys Leu Glu His Leu Arg Pro Tyr Ala
225 230 235 240
Gly Asp Phe Val Ala Asp Asp Thr Gln Arg Arg Leu Asp Leu Leu Lys
245 250 255
Pro Arg Leu Pro Asn Gly Glu Cys Pro Pro Gly Phe Leu Gly Phe Ala
260 265 270
Val Asn Met Ile Asn Val Asp Ser Ala Asn Ile Leu Cys Leu Thr Ser
275 280 285
Ser Gly Cys Gly Leu Arg Glu Thr Leu Phe Tyr Asn Leu Phe Ser Arg
290 295 300
Leu Gln Val Tyr Arg Thr Arg Ala Glu Met Leu Leu Ala Leu Pro Cys
305 310 315 320
Ile Thr Asp Gly Ala Leu Ser Leu Asp Gly Gly Met Ile Lys Thr Ala
325 330 335
Gly Val Phe Ser Leu Gly Ser Arg Glu Asp Val Glu Val Arg Phe Pro
340 345 350
Lys Ser Ser Gly Ser Asn Leu Pro Leu Glu Tyr Phe Glu Thr Glu Lys
355 360 365
Glu Leu Thr Glu Val Asn Trp Lys Arg Glu Lys Val Glu Glu Asp Ile
370 375 380
Gln Arg Glu Gln Ser Leu Leu Asn His Ile Asn Tyr Thr Phe Arg Ile
385 390 395 400
Lys Lys Gln Ala Phe Ile Lys Tyr Leu Ala Asp Ser Ser Pro Tyr Gly
405 410 415
Ile Gln Gln Gln Ile Pro Gly Glu Arg Glu Arg Leu Thr Gln Arg
420 425 430
<210> 46
<211> 1596
<212> PRT
<213> 可可(Theobroma cacao)
<400> 46
Met Gln Ser Gln Phe Asn Ala Met Glu Asp Lys Phe His Arg Arg Ala
1 5 10 15
Ser Lys Arg Pro Leu Val Leu Glu Asp Glu Asp Asp Ala Asp Gly Ser
20 25 30
Asn Glu Val Tyr Arg Phe Lys Val Leu Leu Pro Asn Gly Thr Ser Val
35 40 45
Asp Leu Ser Leu Lys Asn Gln Lys Pro Glu Ile Ser Phe Glu Asp Phe
50 55 60
Ile Asp Leu Ile Arg Val Glu Tyr Asp Tyr Ile Val Arg Ser Gln Arg
65 70 75 80
Gln Ser Val Lys Arg Lys Arg Ile Ile Asn Trp Asn Ser Glu Lys Leu
85 90 95
Tyr Leu Glu Gly Asp Met Gly Gly Lys Ile Met Ser Arg Ile Lys Leu
100 105 110
Arg His Phe Lys Pro Leu Lys Cys His Ile Leu Arg Leu His Asp Gly
115 120 125
Ser Gly Glu Val Ala Asn Thr Tyr Glu Asn Met Trp Asp Leu Thr Pro
130 135 140
Asp Thr Asp Leu Leu Met Glu Leu Pro Glu Glu Tyr Thr Phe Glu Thr
145 150 155 160
Ala Leu Ala Asp Leu Ile Asp Asn Ser Leu Gln Ala Val Trp Leu Asn
165 170 175
Gly Lys Asn Glu Arg Arg Leu Ile Ser Val Asn Val Leu Glu Asn Thr
180 185 190
Ile Ser Ile Phe Asp Thr Gly Pro Gly Met Asp Ser Ser Asp Glu Asn
195 200 205
Ser Ile Val Lys Trp Gly Lys Met Gly Ala Ser Leu Asn Arg Leu Ser
210 215 220
Lys Val Gln Ala Ile Gly Cys Lys Pro Pro Tyr Leu Met Pro Phe Phe
225 230 235 240
Gly Met Phe Gly Tyr Gly Gly Pro Ile Ala Ser Met His Leu Gly Ser
245 250 255
Cys Ala Ile Val Ser Ser Lys Thr Lys Glu Ser Lys Lys Val Tyr Thr
260 265 270
Leu Gln Ile Ala Arg Glu Ala Leu Leu Asn Asn Ser Asn Pro Glu Arg
275 280 285
Cys Trp Arg Thr Asp Gly Gly Ile Arg Asp Ala Ser Glu Asp Glu Ile
290 295 300
Glu Lys Ser Pro His Gln Ser Phe Thr Lys Val Glu Ile Leu Lys Pro
305 310 315 320
Lys Gln Lys Asn Leu Asp Ile Phe Lys Leu Gln Cys Lys Leu Lys Asp
325 330 335
Thr Tyr Phe Pro Tyr Ile Gln Cys Asp Glu Leu Ser Lys Val Gly Arg
340 345 350
Thr Ile Thr Pro Val Glu Phe Gln Val Val Asn Gly Val Asp Leu Thr
355 360 365
Glu Ile Asp Gly Gly Glu Ala Ala Ile Thr Asn Leu Leu Ser Cys Asn
370 375 380
Gly Pro Glu Phe Ser Ile Leu Leu His Phe Ser Leu Arg Arg Glu Asn
385 390 395 400
Val Ala Thr Lys Gly Ser Lys Ala Ser Gln Glu Ala Asn Ala Arg Leu
405 410 415
Lys Cys Ile Tyr Phe Pro Ile Arg Gln Gly Lys Glu Asn Ile Glu Arg
420 425 430
Ile Leu Glu Arg Leu Gly Ala Glu Gly Cys Gly Val Arg Glu Asn Tyr
435 440 445
Glu Asp Phe Ser Arg Val Ser Ile Arg Arg Leu Gly Arg Leu Leu Pro
450 455 460
Asp Ala Arg Trp Ala Leu Leu Pro Phe Met Asp Leu Arg Gln Arg Lys
465 470 475 480
Gly Asp Lys Ser His Leu Leu Lys Arg Cys Cys Leu Arg Val Lys Cys
485 490 495
Phe Val Glu Thr Asp Ala Gly Phe Asn Pro Thr Pro Ser Lys Thr Asp
500 505 510
Leu Ala His His Asn Pro Phe Ser Ile Ala Leu Lys Asn Phe Gly Ser
515 520 525
Arg His Val Glu Lys Glu Lys Asp Val Asp Val Asp Ile Tyr Arg Gly
530 535 540
Gly Lys Gln Leu Thr Phe Leu Gln Leu Glu Arg Glu Tyr Gln Asp Trp
545 550 555 560
Leu Leu Leu Met His Asp Ser Tyr Asp Glu Glu Ile Val Ser Gly Glu
565 570 575
Asp Gln Pro Val Leu Val Val Gly Pro Leu Asn Lys Lys Ala Leu Gly
580 585 590
Ile Ser Ser Asp Val Ile Arg Val His Lys Ile Leu Lys Arg Lys Gly
595 600 605
Val Leu Trp Lys Arg Arg Gln Arg Ile Lys Val Leu Lys Gly Ala Cys
610 615 620
Ala Gly Phe His Lys Asn Asn Val Tyr Ala Thr Leu Glu Tyr Phe Leu
625 630 635 640
Ile Glu Gly Phe Gln Gly Asp Phe Gly Gly Glu Ala Arg Ile Ile Cys
645 650 655
Arg Pro Leu Gly Leu Ser Asn Gly Ser Ile Leu Ser Val Lys Asp Gly
660 665 670
Asn Ala Ser Phe Asp Ile Arg Ser Ser Leu Ser Leu Pro Val Ser Val
675 680 685
Ile Asp Ser Gly Lys Cys Leu Ala Ile Asp Asp Thr Asp Trp Asp Cys
690 695 700
Gln Leu Glu Lys Gln Cys Gln Lys Ala Pro Ser Arg Ile Asp Leu Leu
705 710 715 720
Asn Ala Lys Gln Cys Gln Glu Leu Glu Val Asp Gly Ala Leu Pro Ala
725 730 735
Asp Ala Thr Val His Ala Gly Leu Val Pro Pro Lys Glu Ile Val Ala
740 745 750
Val Leu Arg Pro Arg Ser Phe Gly Ser Ser Ser Ala Ser Asn Asp Leu
755 760 765
Glu Gln Lys Asp Ile Leu Lys Ile Asn Leu Glu Met Ser Met Glu Val
770 775 780
Asn Phe Arg Arg Thr Lys Asn His Gln Asp Val Lys His Ile Tyr Ser
785 790 795 800
Gly Arg Ile Thr Pro Ser Ser His Lys Gly Phe Asn Gly Leu Tyr Val
805 810 815
Phe Pro Ile Gly Ser Lys Phe Thr His Leu Phe Gln Val Ala Gly Leu
820 825 830
Tyr Thr Phe Leu Phe Ser Ile Glu His Ser Gly Cys Gln Asp Cys Lys
835 840 845
Lys Thr Leu Leu Val Val Pro Ser Leu Lys Val Gly Lys Trp Arg Leu
850 855 860
Leu Ser Asp Gly Lys Ile Pro Ser Tyr Asn Val Arg Val Gly Ser Cys
865 870 875 880
Phe Ala Leu Ile Pro Ile Ala Cys Tyr Asp Ile Tyr Gly Asn Arg Met
885 890 895
Pro Phe Ser Ser Ile Pro Asn Phe Lys Ile Lys Leu Val Met Asn Glu
900 905 910
Gly Met Leu Val Asp Val Thr Gln Met Lys Pro Ser Leu Ser Ser Asp
915 920 925
Asn Leu Val Leu Asn Ile Glu Asp Val Met Ile Glu Ser Asn Gly Leu
930 935 940
Asp Ser Met Arg Pro His Tyr Ala Ala Thr Leu Val Ile Tyr Ser Lys
945 950 955 960
Asp Glu Ser Val Ser Ile Ser Val Glu Cys Gln Val Thr Pro Gly Ala
965 970 975
Leu Arg Asn Val Arg Ala Cys Pro Glu Val Leu Gly Asn Gln Leu Leu
980 985 990
Pro Gly Phe Ile Ile Glu Gln Leu Val Leu Glu Met Phe Asp Ala Tyr
995 1000 1005
Gly Asn His Val Ala Glu Gly Ala Glu Val Gln Phe His Leu Asp Gly
1010 1015 1020
Phe Val Ile Gln Gly His Leu Gly Ser Lys Tyr Lys Val Asp Asp Arg
1025 1030 1035 1040
Gly Cys Ile Asp Leu Gly Gly Leu Leu Glu Val Thr Ala Gly Tyr Gly
1045 1050 1055
Lys Ser Val Ser Leu Ser Val Leu His Asp Gly Lys Val Val Phe Lys
1060 1065 1070
Arg Glu Phe Gln Thr Glu Lys Arg Glu Leu Arg Ile Ala Ser Val Val
1075 1080 1085
Pro Glu Arg Cys Ile Ala Gly Ser Ile Leu Glu Asp Leu Ala Phe Glu
1090 1095 1100
Val Val Asp Ser Gln Gly Val Val Asp Glu Thr Phe His Asp Asp Glu
1105 1110 1115 1120
Lys His Gly Gln Ser His Arg Leu Ile Val Asn Ser Glu Ser Phe Glu
1125 1130 1135
Thr Cys Asp Ser Ile Cys Tyr Ala Phe Ile His Gly Cys Cys Ile Val
1140 1145 1150
Thr Ser Ile Pro Leu Pro Glu Ile Glu Gly Pro Phe Cys Phe Val Ala
1155 1160 1165
Phe His Ser Arg Tyr Met Asp Leu Tyr Leu Asn Val Lys Val Ser Leu
1170 1175 1180
Val Arg Pro Arg Lys Val Glu Ser Asp Glu Ile Glu Tyr Pro Ser Asp
1185 1190 1195 1200
Gln Lys Gly Leu Phe Leu Gln Lys Ser Gln Ser Val Lys Asp Val Gly
1205 1210 1215
Cys Leu Leu Ser Leu Val Lys Tyr Asp Lys Glu Leu Glu Asp Glu Val
1220 1225 1230
Cys Lys Tyr Gly Glu Arg Ile Ala Lys Trp Glu His Leu Leu Glu Thr
1235 1240 1245
Leu Asp Cys Arg Lys Ala Ser Ile Glu Arg Tyr Val Ser Gly Leu Gln
1250 1255 1260
Ala Ser Leu Glu Pro Asn Leu Ile Asp Asn Leu Asp Ser Leu Ser Thr
1265 1270 1275 1280
Lys Glu Glu Met Met Ile Arg Ile Lys Glu Arg Asp His Ser Ala Ala
1285 1290 1295
Ser Val Leu Cys Ser Leu Ala Gln Lys Leu Pro Phe Gln Glu Pro Trp
1300 1305 1310
Met Asp Val Ile Glu Gly Leu Val Gly Val Val Ala Leu Leu Gly Thr
1315 1320 1325
Val Cys Thr Ser Lys Leu Ser Arg Ile Leu Ala Glu Tyr Leu Gly Glu
1330 1335 1340
Asp Gln Met Leu Ala Val Val Cys Lys Ser Tyr Thr Ala Ala Arg Ala
1345 1350 1355 1360
Leu Glu Lys Tyr Glu His Asn Gly Lys Val Asp Trp Lys Leu Gly Leu
1365 1370 1375
His Ala Glu Ala Thr Ala Leu Gly Lys Ser Ile Ser Gly Arg Phe Leu
1380 1385 1390
Val Val Cys Leu Glu Asp Ile Arg Pro Tyr Pro Gly Leu Ile Glu Val
1395 1400 1405
Ser Asp Pro Gln Arg Lys Leu Ala Leu Pro Asp Pro Arg Leu Pro Thr
1410 1415 1420
Gly Asn Thr Pro Pro Gly Phe Ile Gly Tyr Ala Val Asn Met Val Asn
1425 1430 1435 1440
Ile Asp His Pro His Leu Glu Asn Leu Thr Thr Ala Gly His Gly Leu
1445 1450 1455
Arg Glu Thr Leu Phe Tyr Arg Leu Phe Ser Lys Leu Gln Val Tyr Glu
1460 1465 1470
Thr Arg Glu His Met Glu Asn Ala Arg Ala Cys Ile Lys His Ser Ala
1475 1480 1485
Ile Ser Leu Asp Gly Gly Ile Leu Arg Lys Asn Gly Ile Ile Ser Leu
1490 1495 1500
Gly Tyr Arg Asn Pro Glu Ile His Phe Pro Val Gln Met His Val Ser
1505 1510 1515 1520
Gln Gln His Lys Glu Ile Met Glu Gln Ile Lys Lys Met Lys Leu Glu
1525 1530 1535
Leu Arg Ser Ile Leu Gln His Ile Glu Arg Ile Ser Glu Asn His Ala
1540 1545 1550
Lys Ala Ser Lys Lys Phe Asn Lys Arg Lys Met Lys Leu Glu Lys Cys
1555 1560 1565
Met Asp Arg Met Asp Ser Thr Ile Lys Tyr Tyr His Val Glu Tyr Ala
1570 1575 1580
Pro Asn Thr Leu Lys Ser Glu Glu Ala Pro Pro Met
1585 1590 1595
<210> 47
<211> 1261
<212> PRT
<213> 大豆
<400> 47
Met Val Leu Phe Glu Met Gln Asn Met Trp Asp Ser Thr Pro Asp Thr
1 5 10 15
Asp Leu Leu Leu Glu Leu Pro Glu Glu Tyr Thr Phe Glu Thr Ala Leu
20 25 30
Ala Asp Leu Ile Asp Asn Ser Leu Gln Ala Val Trp Ser Asn Gly Glu
35 40 45
Asp Lys Arg Lys Leu Ile Arg Val Asn Leu Gly Lys Ser Lys Ile Ser
50 55 60
Ile Phe Asp Asn Gly Pro Gly Leu Asp Asp Thr Asp Glu Asn Ser Leu
65 70 75 80
Val Lys Trp Gly Lys Met Asp Ala Ser Leu His Arg Ser Ser Lys Ser
85 90 95
Lys Ala Ile Gly Gly Lys Pro Pro Tyr Leu Met Pro Tyr Phe Gly Met
100 105 110
Phe Gly Tyr Gly Gly Pro Ile Ala Ser Met His Leu Gly Arg Arg Ala
115 120 125
Ser Val Ser Tyr Lys Thr Lys His Val Lys Lys Val Tyr Thr Leu His
130 135 140
Leu Glu Arg Glu Ala Leu Leu Asn Thr Ser Ser Ser Gln Leu Thr Trp
145 150 155 160
Lys Thr Gly Gly Gly Ile Arg Asp Pro Leu Ala Asn Glu Ile Arg Asp
165 170 175
Ser His Gly Ser Phe Thr Lys Gly Glu Ser Ser Val Thr Glu Tyr Phe
180 185 190
Asn Val Leu Tyr Arg His Trp Gln Gln Leu Asp Ile Tyr Glu Lys Val
195 200 205
Ser Arg Cys Gly Thr Lys Asp Lys Lys Lys Tyr Lys Glu Leu Gly Thr
210 215 220
Asp Arg Ile Tyr Lys Phe Leu Leu Gly Leu Asn Lys Asp Leu Asp Glu
225 230 235 240
Val Arg Gly Arg Ile Leu Gly Phe Lys Pro Leu Pro Lys Ile Arg Glu
245 250 255
Val Phe Ser Lys Gln Glu Glu Thr Ser Leu Asp His His Lys Arg Lys
260 265 270
Thr Thr Pro Gly Val Ile Ile Val Arg Gly Gln Ala Thr Pro Arg Lys
275 280 285
His Ala Gly Ser Ser Met Gln Phe Lys Ser Lys Glu Gly Arg Gly Asn
290 295 300
Thr Ala Phe Asn His Gln Gln Ala Glu Val Asn Ser Asn Ser Ser Ser
305 310 315 320
Phe Ser Lys Glu Gln Met Glu Ala Leu Gln Thr Leu Phe Gln Gln Ser
325 330 335
Met Ile Leu Glu Leu Gly Lys Thr Ile Gly Asn Ala Glu Glu Cys Val
340 345 350
Gly Leu Tyr Leu Phe Gln Val Glu Lys Pro Lys Glu Leu Lys Asn His
355 360 365
Ser Tyr Val Ala Ala Ser Val Pro Ser Ser Asp Asn Ser Val Met Trp
370 375 380
His Cys Arg Leu Gly His Pro Asn Phe Ile Leu Asn Arg Tyr Lys Val
385 390 395 400
Arg Phe Val Ala Lys Gly Phe Thr Gln Ser Tyr Gly Val Asp Tyr Glu
405 410 415
Glu Thr Phe Ala Leu Val Ala Lys Leu Asn Phe Val Arg Cys Asp Asp
420 425 430
Thr Ser Asp Arg Gly Lys Thr Ile Thr Pro Ile Glu Phe Lys Val Asn
435 440 445
Asp Val Asp Leu Thr Glu Ile Gln Gly Gly Glu Val Ala Ile Thr Asn
450 455 460
Trp His Ser Cys Asn Gly Pro Glu Phe Val Phe Gln Leu His Leu Lys
465 470 475 480
Ser Glu Ser Ser Arg Glu Leu Gln Ala Asn Ala Arg Met Arg Phe Val
485 490 495
Tyr Leu Pro Phe Thr Lys Gly Lys Glu Asn Ile Glu Arg Val Leu Glu
500 505 510
Lys Leu Lys Ser Asp Gly Phe Val Ile Ser Glu Asp Phe Gln Ser Phe
515 520 525
Ser Arg Val Ser Val Arg Arg Leu Gly Arg Leu Leu Pro Asp Ala Arg
530 535 540
Trp Thr Leu Leu Pro Phe Met Asp Phe Arg Asn Lys Lys Gly Ile Lys
545 550 555 560
Ala Gln Ile Leu Lys Arg Cys Ser Gln Arg Val Lys Cys Phe Ile Glu
565 570 575
Thr Asp Gly Gly Phe Lys Pro Thr Leu Ser Lys Thr Asp Leu Ala His
580 585 590
His Asn Pro Phe Thr Ala Ala Leu Lys Asn Phe Gly Asn Lys Phe Ser
595 600 605
Glu Lys Glu Lys Asp Val Thr Val Glu Ile Arg Lys Ala Thr Lys Val
610 615 620
Leu Thr Leu Leu Lys Leu Gln Met Glu Tyr Gln Asp Trp Ile Leu Gln
625 630 635 640
Met His His Gln Tyr Asp Glu Glu Ala Asp Ser Gly Glu Asp Gln Pro
645 650 655
Val Ile Ile Val Gly Pro Ala Asn Glu Lys Ala Leu Gly Ile Ser Ser
660 665 670
Asp Val Ile Arg Val His Gln Val Leu Asn Arg Lys Glu Lys Ser Trp
675 680 685
Lys Arg Gly Gln Lys Ile Lys Val Leu Lys Gly Ala Cys Ala Gly Cys
690 695 700
His Arg Thr Thr Ile Tyr Ala Thr Ile Glu Tyr Phe Leu Leu Glu Gly
705 710 715 720
Phe Glu Gly Asp Ala Gly Gly Glu Ala Arg Ile Ile Cys Arg Ala Ile
725 730 735
Asp Ile Pro Asp Glu Asn Gly Ser Phe Leu Ser Val Gly Asp Glu Asp
740 745 750
Ala Ser Leu Glu Ile Arg Gly Ser Leu Ser Leu Pro Ile Ser Val Ile
755 760 765
Asp Ser Gly Lys Val Ile Ala Val Glu Ser Ile Glu Trp Glu Asn Arg
770 775 780
Leu Thr Lys Lys Gln Gln Lys Ser Thr Ser Ile Tyr Leu Pro Gly Ala
785 790 795 800
Asn His Tyr Glu Asn Leu Glu Thr Asn Gly Val Val Ser Val Glu Ser
805 810 815
Ile Glu Trp Glu Asn Arg Leu Tyr Lys Lys Gln Gln Lys Ser Pro Ser
820 825 830
Ile Asn Leu Ser Gly Ala Asn His Tyr Glu His Leu Glu Ala Asp Gly
835 840 845
Val Pro Val Ile Lys Met Pro Ile Val Lys Tyr Ala Gly Gln Pro Pro
850 855 860
Cys Thr Asp Lys Gly Ile Ile Asp Leu Gln Glu Pro Ser Leu Tyr His
865 870 875 880
Glu Asn Ser Leu Ile Glu Phe Phe Leu Asn Tyr Asp Lys Glu Leu Phe
885 890 895
Asp Ser Ile Cys Lys Leu Ala Glu Arg Ile Gln Lys Val Glu Ser His
900 905 910
Leu Asn Asn Ser Asn Glu Lys Lys Ala Glu Thr Glu Gln Glu Met Val
915 920 925
Lys Leu Leu Glu Lys Val Glu Pro Tyr Gln Leu Ser Ile Met Asp Ser
930 935 940
Ser Phe Thr Lys Asp Glu Leu Met Thr Lys Ile Arg Ser Met Glu Asn
945 950 955 960
Ser Pro Tyr Ser Val Leu Cys Ser Leu Ser Lys Arg Glu Lys Pro Pro
965 970 975
Asn Tyr Phe Leu Glu Asp Leu Ile Gly Val Val Ala Leu Ile Gly Thr
980 985 990
Val Gln Arg Pro Glu Leu Ser Arg Ile Leu Ala Glu Tyr Leu Gly Glu
995 1000 1005
Ala Lys Met Leu Gly Leu Ile Tyr Arg Ser Phe Asp Thr Ala Ser Ser
1010 1015 1020
Leu Glu Lys Tyr Asn Gln Lys Gly Glu Ile Asp Tyr Glu Arg Ala Leu
1025 1030 1035 1040
His Ala Glu Ala Ala Ala Leu Gly Lys Ala Ile Ser Asn Arg Phe His
1045 1050 1055
Val Ile Cys Phe Glu Asp Ile Arg Pro Tyr Thr Gly Trp Leu His Asp
1060 1065 1070
Asp Ser Gln Arg Arg Leu Ala Leu Pro Asn Pro Arg Ile Ala Asn Gly
1075 1080 1085
Glu Thr Pro Glu Gly Phe Ile Gly Tyr Ala Val Asn Met Val Asp Leu
1090 1095 1100
Asp Ile Asn Ser Leu Gln Ile Met Thr Ala Ser Asp Phe Gly Leu Arg
1105 1110 1115 1120
Glu Thr Val Leu Phe Asn Leu Phe Lys Lys Leu Gln Val Tyr Asn Thr
1125 1130 1135
Arg Glu Asn Met Val Ala Ala Arg Thr Cys Ile Glu Asp Gly Ala Val
1140 1145 1150
Ser Leu Asp Gly Gly Ile Leu Ser Glu Asn Gly Ile Leu Ser Leu Gly
1155 1160 1165
Tyr Gly Asn Pro Ser Ile Cys Phe Pro Cys Glu Asn Gln Lys Val Leu
1170 1175 1180
Pro Arg Glu Ile Glu Lys Ile Leu Pro Gln Met Glu Gly Lys Lys Ser
1185 1190 1195 1200
Asp Leu Arg Met Ile Glu Glu Arg Ile Lys Gly Leu Glu His Ser Arg
1205 1210 1215
Ala Lys Tyr Ile Lys Lys Phe Asn Lys Lys Lys Glu Arg Tyr Asp Lys
1220 1225 1230
Val Thr Asp Arg Met Lys Pro Met Thr Glu Leu Leu Lys Tyr Lys Pro
1235 1240 1245
Glu Ala Gly Asp Gly Ser Asn Ser Ser Gly Arg Leu Ser
1250 1255 1260
<210> 48
<211> 418
<212> PRT
<213> 稻
<220>
<223> 粳型（Japonica group）
<400> 48
Met Ala Val Gln Ser Ala Glu Arg Gly Val Glu Ala Pro Pro Leu Thr
1 5 10 15
Leu Ser Ile Phe Glu Phe Asn Ser Lys Val Met Gln Asp Glu Leu Gln
20 25 30
Lys Leu Ala Leu Lys Val Asn His His Glu Glu Asn Ile Arg Phe Leu
35 40 45
Lys Ser Glu Leu Asn Ala Val Glu Asp Ser Cys Ala Asp Leu Gly Ile
50 55 60
Lys Ile Gly Asn Tyr Tyr Ser Ser Met Ala Ala Ile Ala Asn Asn Asp
65 70 75 80
Thr Ser Val Glu Glu Ala Glu Gln Arg Thr Ile Gln Ser Ile Leu Lys
85 90 95
Gln Asp Glu Thr Ala Ala Gly Ile Ile Cys Gln Leu Lys Ile Arg His
100 105 110
Tyr Gln His Ala Ser Lys Leu Pro Leu Met Lys Asp Val Leu Gly Val
115 120 125
Val Ala Thr Leu Gly Lys Ile Asn Asn Asp Asn Leu Ser Arg Val Leu
130 135 140
Ser Glu Tyr Leu Gly Leu Asp Asn Met Leu Gly Val Val Cys Lys Thr
145 150 155 160
Tyr Asp Gly Val Glu Gly Leu Glu Thr Tyr Asp Lys Glu Gly Met Val
165 170 175
Asp Lys Thr Ser Gly Ile His Gly Leu Gly Arg Thr Ile Gly Lys Phe
180 185 190
Leu Asp Gly Arg Phe Thr Val Phe Cys Leu Glu Asn Ile Arg Pro Phe
195 200 205
Ser Gly Asp Phe Met Ile Asp Asp Pro Gln Arg Lys Leu Leu Leu His
210 215 220
Lys Pro Arg Leu Pro Ser Gly Glu Phe Pro Pro Gly Phe Leu Gly Phe
225 230 235 240
Ala Val Asn Met Ile His Leu Asp Gln Ala Asn Leu Ser Cys Leu Thr
245 250 255
Ala Gly Gly His Gly Leu Arg Glu Thr Leu Phe Tyr Ser Leu Phe Ser
260 265 270
Gln Leu Gln Val Tyr Lys Thr Arg Ala Glu Leu Arg Asn Ala Ile Pro
275 280 285
Leu Ile Asn Asp Gly Ala Val Ser Leu Asp Gly Ser Ile Leu Arg Pro
290 295 300
Asn Gly Ser Phe Cys Leu Gly Asn Arg Ala Ser Leu Gly Ile Gln Phe
305 310 315 320
Pro Val Thr Ala Asn Leu Gly Val Ser Asn Leu Pro Val Thr Ile Thr
325 330 335
Glu Leu Glu Glu Gln Val Lys His Lys Asn Trp Glu Lys Glu Arg Leu
340 345 350
Leu Glu Asp Ile Lys Arg Gln Glu Asp Leu Leu Asn Gln Val Lys Asp
355 360 365
Leu Phe Ser Lys Lys Lys Glu Gln Phe Met Ala Tyr Ile Thr Gln Pro
370 375 380
Gly Met Leu Gln Arg Ala Ser Gln Ala Ser Pro Thr Ile Pro Ser Pro
385 390 395 400
Ala Thr Pro Gly Ser Asn Pro Phe Gly Ser Arg Pro Pro His Leu Arg
405 410 415
Gly Arg
<210> 49
<211> 344
<212> PRT
<213> 稻
<220>
<223> 籼型（Indica group）
<400> 49
Met Ala Ala Ile Ala Asn Asn Asp Thr Ser Val Glu Glu Ala Glu Gln
1 5 10 15
Arg Thr Ile Gln Ser Ile Leu Lys Gln Asp Glu Thr Ala Ala Gly Ile
20 25 30
Ile Cys Gln Leu Lys Ile Arg His Tyr Gln His Ala Ser Lys Leu Pro
35 40 45
Leu Met Lys Asp Val Leu Gly Val Val Ala Thr Leu Gly Lys Ile Asn
50 55 60
Asn Asp Asn Leu Ser Arg Val Leu Ser Glu Tyr Leu Gly Leu Asp Asn
65 70 75 80
Met Leu Gly Val Val Cys Lys Thr Tyr Asp Gly Val Glu Gly Leu Glu
85 90 95
Thr Tyr Asp Lys Glu Gly Met Val Asp Lys Thr Ser Gly Ile His Gly
100 105 110
Leu Gly Arg Thr Ile Gly Lys Phe Leu Asp Gly Arg Phe Thr Val Phe
115 120 125
Cys Leu Glu Asn Ile Arg Pro Phe Ser Gly Asp Phe Met Ile Asp Asp
130 135 140
Pro Gln Arg Lys Leu Leu Leu His Lys Pro Arg Leu Pro Ser Gly Glu
145 150 155 160
Phe Pro Pro Gly Phe Leu Gly Phe Ala Val Asn Met Ile His Leu Asp
165 170 175
Gln Ala Asn Leu Ser Cys Leu Thr Ala Gly Gly His Gly Leu Arg Glu
180 185 190
Thr Leu Phe Tyr Gly Leu Phe Ser Gln Leu Gln Val Tyr Lys Thr Arg
195 200 205
Ala Glu Leu Arg Asn Ala Ile Pro Leu Ile Asn Asp Gly Ala Val Ser
210 215 220
Leu Asp Gly Ser Ile Leu Arg Pro Asn Gly Ser Phe Cys Leu Gly Asn
225 230 235 240
Arg Ala Ser Leu Gly Ile Gln Phe Pro Val Thr Ala Asn Leu Gly Val
245 250 255
Ser Asn Leu Pro Val Thr Ile Thr Glu Leu Glu Glu Gln Val Lys His
260 265 270
Lys Asn Trp Glu Lys Glu Arg Leu Leu Glu Asp Ile Lys Arg Gln Glu
275 280 285
Asp Leu Leu Asn Gln Val Lys Asp Leu Phe Ser Lys Lys Lys Glu Gln
290 295 300
Phe Met Ala Tyr Ile Thr Gln Pro Gly Met Leu Gln Arg Ala Ser Gln
305 310 315 320
Ala Ser Pro Thr Ile Pro Ser Pro Ala Thr Pro Gly Ser Asn Pro Phe
325 330 335
Gly Ser Arg Pro Pro His Leu Arg
340
<210> 50
<211> 405
<212> PRT
<213> 玉蜀黍
<400> 50
Met Ala Thr Pro Ser Leu Pro Thr Gln Ile Val Glu Phe Asn Tyr Lys
1 5 10 15
Ala Ile Glu Asp Lys Leu Lys Arg Leu Ala Leu Glu Val Ser His His
20 25 30
Glu Asn Asn Ile Arg Phe Leu Lys Ser Gln Leu Asn Thr Val Glu Glu
35 40 45
Ala Cys Val Asp Leu Gly Ile Lys Leu Gly Asn Tyr His Ser Ser Val
50 55 60
Ala Ala Val Val Asn Asn Asp Thr Ser Ala Gln Glu Ala Glu Gln Arg
65 70 75 80
Thr Ile Arg Ser Ile Leu Asp Gln Gly Lys Thr Ala Ala Gly Ile Ile
85 90 95
Cys Gln Leu Lys Val Arg His His Glu Leu Ala Ser Lys Met Pro Leu
100 105 110
Met Lys Asp Ile Leu Gly Phe Val Ala Thr Leu Gly Lys Val Asn Asn
115 120 125
Asp Asn Leu Ser Arg Leu Leu Thr Glu Tyr Leu Gly Met Asp Asn Met
130 135 140
Leu Ala Leu Val Cys Lys Thr Tyr Asp Gly Val Lys Gly Leu Glu Lys
145 150 155 160
Tyr Asp Lys Asp Gly Ile Ile Asp Asn Thr Ser Gly Ile His Gly Leu
165 170 175
Gly Arg Ser Val Gly Lys Phe Leu Asp Gly Arg Phe Thr Val Phe Cys
180 185 190
Leu Glu Asn Leu Arg Pro Phe Ser Gly Asp Val Ile Ile Asp Asp Pro
195 200 205
Gln Arg Lys Leu Ile Leu His Arg Pro Arg Leu Pro Gly Gly Glu Ser
210 215 220
Pro Pro Gly Phe Leu Asp Phe Ala Val Asn Met Ile His Leu Asp Arg
225 230 235 240
Ala His Leu Arg Phe Leu Thr Ala Ser Gly His Gly Leu Arg Glu Thr
245 250 255
Leu Phe Tyr Thr Leu Phe Ser His Leu Gln Val Tyr Lys Thr Arg Thr
260 265 270
Asp Ile Gln Ser Ala Leu Pro Leu Ile Lys Asp Gly Ala Val Ser Leu
275 280 285
Asp Gly Gly Leu Leu Arg Pro Asn Gly Ser Phe Cys Leu Gly His Ser
290 295 300
Lys Asn Leu Glu Val Lys Phe Pro Val Thr Thr Asp Val Ser Ser Leu
305 310 315 320
Pro Glu Asn Val Ala Glu Met Glu Glu Gln Val Lys Arg Lys Asn Trp
325 330 335
Glu Lys Glu Met Val Leu Glu Asp Met Lys Arg Glu Glu Asp Leu Leu
340 345 350
Lys Gln Val Lys Glu Leu Tyr Arg Lys Gln Lys Gln Glu Leu Met Asp
355 360 365
Tyr Val Thr Gln Pro Ala Val Thr Gln Met Ser Arg Asp Ser Pro Ala
370 375 380
Thr His Ser Pro Ala Thr Pro Gly Ser Asn Pro Phe Gly Ala Lys Leu
385 390 395 400
Ser Asn Lys Trp Arg
405
<210> 51
<211> 388
<212> PRT
<213> 两色蜀黍
<400> 51
Met Glu Asp Lys Leu Lys Arg Leu Ala Leu Lys Val Gly His His Glu
1 5 10 15
Asp Asn Ile Arg Phe Leu Lys Ser Gln Leu Asn Ala Val Glu Asp Ala
20 25 30
Cys Ile Asp Leu Gly Ile Lys Leu Gly Asn Tyr His Ser Ser Val Ala
35 40 45
Ala Val Met Asn Asn Asp Thr Ser Ala Gln Glu Ala Glu Gln Arg Thr
50 55 60
Ile Arg Ser Ile Leu Asp Gln Asp Thr Thr Ala Ala Gly Ile Ile Cys
65 70 75 80
Gln Leu Lys Val Arg His His Glu Leu Ala Ser Lys Met Thr Leu Met
85 90 95
Lys Gly Ile Leu Gly Phe Val Ala Thr Leu Gly Lys Val Asn Asn Asp
100 105 110
Asn Leu Ser Arg Leu Leu Thr Glu Tyr Leu Gly Met Asp Asn Met Leu
115 120 125
Ala Leu Val Cys Lys Thr Tyr Asp Gly Val Lys Asp Leu Glu Lys Tyr
130 135 140
Asp Lys Asp Gly Ile Ile Asp Lys Thr Ser Gly Val His Gly Leu Gly
145 150 155 160
Arg Ser Val Gly Lys Phe Leu Asp Gly Arg Phe Thr Val Phe Cys Leu
165 170 175
Glu Asn Leu Arg Pro Phe Ser Gly Asp Ile Asn Ile Gly Asp Pro Gln
180 185 190
Arg Lys Leu Ile Leu His Arg Pro Arg Leu Pro Gly Gly Glu Ser Pro
195 200 205
Pro Gly Phe Leu Asp Phe Ala Val Asn Met Ile His Leu Asp Arg Ala
210 215 220
His Leu Ser Cys Leu Thr Ala Ser Gly His Gly Leu Arg Glu Thr Leu
225 230 235 240
Phe Tyr Thr Leu Phe Ser His Leu Gln Val Tyr Lys Thr Arg Ala Asp
245 250 255
Ile Gln Ser Ala Leu Pro Leu Ile Lys Asp Gly Ala Val Ser Leu Asp
260 265 270
Gly Gly Ile Leu Arg Pro Asn Gly Ser Phe Cys Leu Gly Asp Ser Lys
275 280 285
Asn Leu Glu Val Arg Phe Pro Val Asn Ile Glu Val Ser Ser Leu Pro
290 295 300
Glu Asn Ile Ala Glu Met Glu Glu Gln Val Lys Leu Lys Asn Trp Glu
305 310 315 320
Lys Glu Arg Val Leu Glu Asp Met Lys Arg Glu Glu Asp Leu Leu Lys
325 330 335
Gln Val Lys Glu Leu Tyr Arg Lys Gln Lys Gln Glu Leu Met Asp Tyr
340 345 350
Leu Thr His Pro Ala Val Ala Gln Thr Ser Arg Asp Ser Pro Thr Thr
355 360 365
His Ser Pro Thr Thr Pro Gly Ser Asn Pro Phe Gly Ala Lys Pro Ser
370 375 380
His Lys Gly Trp
385
<210> 52
<211> 658
<212> PRT
<213> 人工序列
<220>
<223> 合成的构建体
<400> 52
Met Tyr Pro Thr Gly Gln Gln Ile Ser Phe Gln Thr Thr Pro Leu Asn
1 5 10 15
Val Gln Asp Pro Thr Arg Met Met Asn Leu Asp Gln Ser Ser Pro Val
20 25 30
Ala Arg Asn Glu Thr Gln Asn Gly Gly Gly Ile Ala His Ala Glu Phe
35 40 45
Ala Met Phe Asn Ser Lys Arg Leu Glu Ser Asp Leu Glu Ala Met Gly
50 55 60
Asn Lys Ile Lys Gln His Glu Asp Asn Leu Lys Phe Leu Lys Ser Gln
65 70 75 80
Lys Asn Lys Met Asp Glu Ala Ile Val Asp Leu Gln Val His Met Ser
85 90 95
Lys Leu Asn Ser Ser Pro Thr Pro Arg Ser Glu Asn Ser Asp Asn Ser
100 105 110
Leu Gln Gly Glu Asp Ile Asn Ala Gln Ile Leu Arg His Glu Asn Ser
115 120 125
Ala Ala Gly Val Leu Ser Leu Val Glu Thr Leu His Gly Ala Gln Ala
130 135 140
Ser Gln Leu Met Leu Thr Lys Gly Val Val Gly Val Val Ala Lys Leu
145 150 155 160
Gly Lys Val Asn Asp Glu Asn Leu Ser Gln Ile Leu Ser Asn Tyr Leu
165 170 175
Gly Thr Arg Ser Met Leu Ala Val Val Cys Arg Asn Tyr Glu Ser Val
180 185 190
Thr Ala Leu Glu Ala Tyr Asp Asn His Gly Asn Ile Asp Ile Asn Ala
195 200 205
Gly Leu His Cys Leu Gly Ser Ser Ile Gly Arg Glu Ile Gly Asp Ser
210 215 220
Phe Asp Ala Ile Cys Leu Glu Asn Leu Arg Pro Tyr Val Gly Gln His
225 230 235 240
Ile Ala Asp Asp Leu Gln Arg Arg Leu Asp Leu Leu Lys Pro Lys Leu
245 250 255
Pro Asn Gly Glu Cys Pro Pro Gly Phe Leu Gly Phe Ala Val Asn Met
260 265 270
Ile Gln Ile Asp Pro Ala Tyr Leu Leu Cys Val Thr Ser Tyr Gly Tyr
275 280 285
Gly Leu Arg Glu Thr Leu Phe Tyr Asn Leu Phe Ser Arg Leu Gln Val
290 295 300
Tyr Lys Thr Arg Ala Asp Met Ile Ser Ala Leu Pro Cys Ile Ser Asp
305 310 315 320
Gly Ala Val Ser Leu Asp Gly Gly Ile Ile Arg Lys Thr Gly Ile Phe
325 330 335
Asn Leu Gly Asn Arg Asp Glu Val Asn Val Arg Phe Ala Lys Pro Thr
340 345 350
Ala Ser Arg Thr Met Asp Asn Tyr Ser Glu Ala Glu Lys Lys Met Lys
355 360 365
Glu Leu Lys Trp Lys Lys Glu Lys Thr Leu Glu Asp Ile Lys Arg Glu
370 375 380
Gln Val Leu Arg Glu His Ala Val Phe Asn Phe Gly Lys Lys Lys Glu
385 390 395 400
Glu Phe Val Arg Cys Leu Ala Gln Ser Ser Cys Thr Asn Gln Pro Met
405 410 415
Asn Thr Pro Arg Lys Gly Gly Arg Ala Lys Leu Gly Thr Asp Tyr Lys
420 425 430
Asp Asp Asp Asp Lys Asp Tyr Lys Asp Asp Asp Asp Lys Asp Tyr Lys
435 440 445
Asp Asp Asp Asp Lys Gly Ser Leu Glu Val Leu Phe Gln Gly Pro Leu
450 455 460
Glu Pro Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe Ser Gln Ser Gly
465 470 475 480
Asp Leu Arg Arg His Gln Arg Thr His Thr Gly Glu Lys Pro Tyr Lys
485 490 495
Cys Pro Glu Cys Gly Lys Ser Phe Ser Arg Ser Asp His Leu Thr Thr
500 505 510
His Gln Arg Thr His Thr Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys
515 520 525
Gly Lys Ser Phe Ser Gln Ser Ser Ser Leu Val Arg His Gln Arg Thr
530 535 540
His Thr Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe
545 550 555 560
Ser Thr Ser Gly Asn Leu Val Arg His Gln Arg Thr His Thr Gly Glu
565 570 575
Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe Ser Gln Arg Ala
580 585 590
Asn Leu Arg Ala His Gln Arg Thr His Thr Gly Glu Lys Pro Tyr Lys
595 600 605
Cys Pro Glu Cys Gly Lys Ser Phe Ser Arg Ser Asp Lys Leu Thr Glu
610 615 620
His Gln Arg Thr His Thr Gly Leu Glu Gly Ser Met Ala Gly Gly Leu
625 630 635 640
Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu Asp Thr Gly
645 650 655
Gly Ser
<210> 53
<211> 663
<212> PRT
<213> 拟南芥
<400> 53
Met Ser His Asp Arg Ser Val Asn Val Ser His Asp Ala Val Ile Ala
1 5 10 15
Lys Pro Glu Arg Gly Thr Met Leu Gln Ser Phe Ser Pro Arg Ser His
20 25 30
Gly Ser Lys Gly Tyr Ser Leu Pro Gln Asp Ser Glu Glu Asn Arg Gly
35 40 45
Ser Val Gly Gln Ser Ala Gly Gln Ser Ser Thr Ser Val Val Asp Gln
50 55 60
Val Arg Ser Pro Ala Asp Asp Ala Gly Val Thr Ser Ser Ser Thr Ile
65 70 75 80
Cys Pro Ala Pro Val Cys Arg Gln Phe Trp Lys Ala Gly Ser Tyr Asn
85 90 95
Asp Glu Leu Ser Ser Lys Ser Gln Gln Pro Asn Gly Lys Asn Tyr Leu
100 105 110
His Val His Pro Met Phe Leu His Ser Asn Ala Thr Ser His Lys Trp
115 120 125
Ala Phe Gly Ala Val Ala Glu Leu Leu Asp Asn Ala Val Asp Glu Ile
130 135 140
Gln Asn Gly Ala Thr Phe Val Ile Val Asp Lys Thr Thr Asn Pro Arg
145 150 155 160
Asp Gly Ala Thr Ala Leu Leu Ile Gln Asp Asp Gly Gly Gly Met Asp
165 170 175
Pro Gln Ala Met Arg His Cys Met Gly Phe Gly Phe Ser Asp Lys Lys
180 185 190
Ser Asp Ser Ala Ile Gly Arg Tyr Gly Asn Gly Phe Lys Thr Ser Thr
195 200 205
Met Arg Leu Gly Ala Asp Val Ile Val Phe Ser Arg His Ser Lys Asn
210 215 220
Gln Thr Leu Thr Gln Ser Ile Gly Leu Leu Ser Tyr Thr Tyr Leu Thr
225 230 235 240
Arg Thr Gly His Asp Arg Ile Val Val Pro Ile Leu Asp Tyr Glu Phe
245 250 255
Asn Ala Ser Ala Gly Glu Phe Lys Thr Leu Gln Asp Arg Glu His Phe
260 265 270
Ile Ser Ser Leu Ser Ile Leu Leu Glu Trp Ser Pro Phe Ser Thr Glu
275 280 285
Ala Glu Leu Leu Gln Gln Phe Asp Asp Val Gly Pro His Gly Thr Lys
290 295 300
Val Ile Ile Tyr Asn Met Trp Leu Asn Ser Asp Ala Lys Leu Glu Leu
305 310 315 320
Asp Phe Asp Ser Val Ala Glu Asp Ile Leu Ile Glu Gly Ser Ile Lys
325 330 335
Lys Thr Gly Ser Lys Ile Val Asn Asp His Ile Ala Ser Arg Phe Ser
340 345 350
Tyr Ser Leu Arg Val Tyr Leu Ser Ile Leu Tyr Leu Arg Ile Pro Glu
355 360 365
Thr Phe Lys Ile Ile Leu Arg Gly Lys Val Val Glu His His Asn Val
370 375 380
Ala Asp Asp Leu Met His Pro Gln Tyr Ile Leu Tyr Lys Pro Gln Ala
385 390 395 400
Ala Gly Ser Glu Glu Ala Leu Val Val Thr Thr Ile Gly Phe Leu Lys
405 410 415
Glu Ala Pro Lys Val Asn Leu His Gly Phe Cys Val Tyr His Lys Asn
420 425 430
Arg Leu Ile Met Pro Phe Trp Gln Val Ile Asn Tyr Ser Ser Ser Arg
435 440 445
Gly Arg Gly Val Val Gly Val Leu Glu Ala Asn Phe Val Glu Pro Thr
450 455 460
His Asn Lys Gln Asp Phe Glu Lys Thr Val Leu Leu Gln Lys Leu Glu
465 470 475 480
Asn Arg Leu Lys Glu Met Thr Val Glu Tyr Trp Ser Cys His Cys Val
485 490 495
Leu Ile Gly Tyr Gln Val Asn Lys Lys Pro Arg Leu Gln Ile Pro Gln
500 505 510
Lys Val Gln Pro Ala Gly Arg Gln Ala Leu Ser Pro Pro Pro Gly Phe
515 520 525
Gln Ala Val Phe Pro Gln Gly Asn Thr Thr Ser Leu Pro Arg Val Ser
530 535 540
Thr Gln Pro Val Leu Leu Glu Lys Arg Lys Glu His Pro Asp Ser Val
545 550 555 560
Ala Ser Ala Ala Leu Lys Arg Lys Val Gly Asn Asp Asp Phe Thr Val
565 570 575
Pro Gly His Ile Arg Val Glu Gln Phe Ile His Gly Ser Ala Ser Gln
580 585 590
Ser Gln Asp Ile Glu Thr Val Lys Leu Met Glu Glu Asn Lys Lys Leu
595 600 605
Arg Ala Lys Cys Leu Asp Arg Lys Val Arg Ser Gln Asn Leu Glu Val
610 615 620
Lys Ala Met Asn Leu Arg Ser Glu Leu Glu Asn Tyr Lys Ser Glu Tyr
625 630 635 640
Glu Arg Leu Met Val Glu Leu Gln Ala Leu Asp Leu Val Lys Asp Glu
645 650 655
His Arg Arg Asn Val Asn Thr
660
<210> 54
<211> 718
<212> PRT
<213> 番茄
<400> 54
Met Ser Ser Thr Asn Met Val Gly Leu Cys Ser Asn Asn Leu Gly Gly
1 5 10 15
Gly Glu Ile Glu Thr Val Lys Leu Glu Pro Ile Leu Val Glu Ser Leu
20 25 30
Ile Pro Gln Met Glu Cys Cys Glu Ala Asp Ser Leu Pro Tyr Lys Lys
35 40 45
Ser Ser Ser Ser Cys Gly Arg Gln Asp Ser Thr Ala Thr Glu Gln Ser
50 55 60
Arg Thr Ser Val Leu Asp Gln Asp Gln Ser Pro Ile Asp Asp Ser Ser
65 70 75 80
Leu Cys Ser Thr Ser Gln Ile Cys Pro Ala Pro Leu Ser Arg Gln Phe
85 90 95
Trp Lys Ala Gly Asn Tyr Asp Glu Gly His Ala Thr Lys Ser Thr Leu
100 105 110
Lys Lys Gly Ser Ser Phe Leu His Ile His Pro Lys Phe Leu His Ser
115 120 125
Asn Ala Thr Ser His Lys Trp Ala Phe Gly Ala Leu Ala Glu Leu Leu
130 135 140
Asp Asn Ala Val Asp Glu Ile Gln Asn Gly Ala Ser Phe Val Ile Val
145 150 155 160
Asp Lys Thr Pro Asn Pro Arg Asn Gly Ser Ser Ala Leu Met Val Gln
165 170 175
Asp Asp Gly Gly Gly Met Asp Pro Glu Ala Ile Arg Arg Cys Met Ser
180 185 190
Phe Gly Phe Ser Asp Lys Lys Ser Ala Ser Thr Ile Gly Gln Tyr Gly
195 200 205
Asn Gly Phe Lys Thr Ser Ser Met Arg Leu Gly Ala Asp Val Ile Val
210 215 220
Phe Ser Arg Cys Lys Lys Asn Arg Lys Leu Thr Gln Ser Ile Gly Leu
225 230 235 240
Leu Ser Gln Thr Phe Leu Thr Arg Ala Gly Leu Asp Lys Ile Val Val
245 250 255
Pro Leu Leu Asp Phe Glu Phe Ser Ala Ala Ala Lys Lys Trp Ser Ser
260 265 270
Leu His Gly Glu Gln His Phe Arg Asn Asn Leu Ser Leu Leu Leu Arg
275 280 285
Trp Ser Pro Phe Ala Thr Glu Ala Glu Leu Leu Lys Gln Phe Asp Asp
290 295 300
Ile Gly Asp His Gly Thr Lys Val Ile Val Tyr Asn Leu Trp Tyr Asn
305 310 315 320
Asp Glu Gly Arg Met Glu Leu Asp Phe Glu Ala Asp Ser Glu Asp Ile
325 330 335
Arg Leu Ser Gly Glu Ala Asn Asp Asn Glu Lys Asn Ala Ser Arg Ile
340 345 350
Lys Val Thr Asp Gln His Leu Ala Asn Arg Leu Arg Tyr Ser Leu Arg
355 360 365
Ala Tyr Leu Ser Val Leu Tyr Leu Arg Ile Pro Asp Asn Phe Cys Ile
370 375 380
Leu Leu Arg Gly Arg Val Val Glu Leu His Asn Ile Ala Lys Asp Leu
385 390 395 400
Lys Tyr Pro Glu Phe Ile Met Tyr Lys Pro Gln Thr Leu Gly Phe Lys
405 410 415
Glu Gly Ser Val Ile Thr Thr Ile Gly Phe Leu Lys Glu Ala Pro Val
420 425 430
Val Asn Ile His Gly Phe Asn Val Tyr His Lys Asn Arg Leu Ile Leu
435 440 445
Pro Phe Trp His Val Val Arg Tyr Ser Asp Ser Arg Gly Arg Gly Val
450 455 460
Val Gly Val Leu Glu Ala Asn Phe Ile Gln Pro Thr His Asn Lys Gln
465 470 475 480
Asp Phe Glu Lys Thr Pro Leu Phe Gln Lys Leu Glu Val Arg Leu Lys
485 490 495
Glu Met Thr Trp Glu Tyr Trp Asp Tyr His Cys Gly Leu Leu Gly Tyr
500 505 510
Phe Pro Lys Lys Arg Pro Gln Gly Pro Ile Thr Pro Gln Ala Ser Ser
515 520 525
Glu Ser Arg Gln Gln His Gly Ser Tyr Lys Pro Ile Val Leu Gly Asn
530 535 540
Ser Ser Gly Ala Val Gly Ser Asn Ile Thr Val Arg Arg Thr Val Thr
545 550 555 560
Ser Glu Ala Ser Ser Glu Ser Arg Gln Gln His Gly Ser Tyr Lys Pro
565 570 575
Ala Val Leu Gly Asn Ser Ser Gly Ala Val Gly Ser Asn Ile Thr Val
580 585 590
Trp Gly Thr Val Thr Pro Val Phe Gln Asn Asn Ser Lys Gln Gly Ala
595 600 605
Ser Lys Arg Lys Ala Val Asp His Pro Ile Glu His Glu Asn Leu Lys
610 615 620
Arg Thr Ala Gly Leu Ile Val Asp Leu Thr Gly Gly Glu Arg Ser Gln
625 630 635 640
Glu Ile Leu Thr Leu Thr Ser Ser Gly Ser Gln Asp Gln Glu Ala Ala
645 650 655
His Leu Ile Gln Glu Asn Glu Lys Leu Arg Ala Gln Cys Cys Glu Cys
660 665 670
Glu Lys Arg Glu Glu Glu Leu His Ser Lys Val Asn Lys Leu Arg Lys
675 680 685
Glu Leu Lys Glu Ala Gln Arg Thr Tyr Ala His Met Leu Val Glu Ser
690 695 700
Arg Leu Leu Asp Lys Val Lys Pro Glu Glu Asp Val Tyr Ile
705 710 715
<210> 55
<211> 830
<212> PRT
<213> 马铃薯
<400> 55
Met Trp Met Gln Glu Asp Asp Leu Val Val Val Glu Lys Asp Pro Gly
1 5 10 15
Met Ser Ser Thr Asn Met Val Gly Leu Cys Ser Asn Asn Leu Gly Gly
20 25 30
Gly Glu Thr Glu Thr Val Lys Leu Glu Pro Glu Leu Val Gly Ser Ser
35 40 45
Leu Pro Gln Met Glu Tyr Cys Glu Ala Asp Ser Leu Pro Tyr Gln Lys
50 55 60
Ser Ser Ser Ser Gly Gly Arg Gln Asp Ser Thr Ala Thr Glu Gln Ser
65 70 75 80
Gly Thr Thr Val Leu Asp Gln Asp Gln Ser Pro Ile Asp Asp Ser Ser
85 90 95
Leu Cys Ser Thr Ser Gln Ile Cys Ser Ala Pro Leu Ser Arg Gln Phe
100 105 110
Trp Arg Ala Gly Asn Tyr Asp Glu Gly Arg Ala Thr Lys Ser Thr Leu
115 120 125
Lys Lys Gly Ser Ser Phe Leu His Ile His Pro Lys Phe Leu His Ser
130 135 140
Asn Ala Thr Ser His Lys Trp Ala Phe Gly Ala Leu Ala Glu Leu Leu
145 150 155 160
Asp Asn Ala Val Asp Glu Ile Gln Asn Gly Ala Ser Phe Val Ile Val
165 170 175
Asp Lys Thr Pro Asn Pro Arg Asn Gly Ser Ser Ala Leu Met Val Gln
180 185 190
Asp Asp Gly Gly Gly Met Asp Pro Glu Ala Ile Arg Arg Cys Met Ser
195 200 205
Phe Gly Phe Ser Asp Lys Lys Ser Ala Ser Thr Ile Gly Gln Tyr Gly
210 215 220
Asn Gly Phe Lys Thr Ser Ser Met Arg Leu Gly Ala Asp Val Ile Val
225 230 235 240
Phe Ser Arg Cys Lys Lys Asn Arg Lys Leu Thr Gln Ser Ile Gly Leu
245 250 255
Leu Ser Gln Thr Phe Leu Thr Arg Ala Gly Leu Asp Arg Ile Val Val
260 265 270
Pro Leu Leu Asp Phe Glu Phe Ser Ala Ala Ala Gly Lys Trp Ser Ser
275 280 285
Leu His Gly Glu Gln His Phe Arg Asn Asn Leu Ser Leu Leu Leu Arg
290 295 300
Trp Ser Pro Phe Val Thr Glu Ala Glu Leu Leu Lys Gln Phe Asp Asp
305 310 315 320
Ile Gly Asp His Gly Thr Lys Val Ile Val Tyr Asn Leu Trp Tyr Asn
325 330 335
Asp Glu Gly Arg Met Glu Leu Asp Phe Glu Ala Asp Ser Glu Asp Ile
340 345 350
Arg Leu Ser Gly Asp Ala Asn Asp Asn Glu Lys Ser Gly Ser Arg Ile
355 360 365
Lys Val Ser Asp Gln His Leu Ala Asn Arg Leu Arg Tyr Ser Leu Arg
370 375 380
Ala Tyr Leu Ser Val Leu Tyr Leu Arg Ile Pro Asp Asn Phe Cys Ile
385 390 395 400
Leu Leu Arg Gly Arg Val Val Glu Leu His Asn Ile Ala Lys Asp Leu
405 410 415
Lys Tyr Pro Glu Phe Ile Met Tyr Lys Pro Gln Thr Leu Gly Phe Lys
420 425 430
Glu Gly Ser Val Ile Thr Thr Ile Gly Phe Leu Lys Glu Ala Pro Val
435 440 445
Val Asn Ile His Gly Phe Asn Val Tyr His Lys Asn Arg Leu Ile Leu
450 455 460
Pro Phe Trp His Val Val Arg Tyr Ser Asp Ser Arg Gly Arg Gly Val
465 470 475 480
Val Gly Val Leu Glu Ala Asn Phe Ile Gln Pro Thr His Asn Lys Gln
485 490 495
Asp Phe Glu Lys Thr Pro Leu Phe Gln Lys Leu Glu Val Arg Leu Lys
500 505 510
Glu Met Thr Trp Glu Tyr Trp Asp Tyr His Cys Gly Leu Leu Gly Tyr
515 520 525
Phe Pro Lys Lys Lys Pro Gln Gly Pro Ile Thr Pro Gln Thr Ser Ser
530 535 540
Glu Ser Arg Gln Leu His Gly Ser Tyr Lys Pro Val Val Leu Gly Asn
545 550 555 560
Ser Ser Gly Ala Val Gly Ser Asn Ile Thr Val Arg Gly Thr Val Thr
565 570 575
Ser Gln Val Ser Ser Glu Ser Arg Gln Gln His Gly Ser Tyr Lys Pro
580 585 590
Val Val Leu Gly Asn Ser Ser Gly Ala Val Gly Ser Asn Ile Thr Val
595 600 605
Arg Gly Thr Val Thr Ser Gln Ala Ser Ser Glu Ser Arg Leu Gln Gln
610 615 620
Gly Ser Tyr Lys Pro Val Val Leu Gly Asn Ser Ser Gly Ala Val Gly
625 630 635 640
Ser Asn Ile Thr Val Arg Gly Thr Val Thr Ser Gln Ala Ser Ser Glu
645 650 655
Ser Arg Leu Gln Gln Gly Ser Tyr Lys Pro Gly Cys Pro Gly Lys Leu
660 665 670
Leu Trp Leu His Leu Asn Leu Asp Tyr Asn Lys Gly His Ile Asn Leu
675 680 685
Val Val Leu Gly Asn Ser Ser Gly Ala Val Gly Ser Asn Ile Thr Val
690 695 700
Arg Gly Thr Val Thr Pro Val Phe Gln Asn Asn Ser Lys Gln Gly Ala
705 710 715 720
Ser Lys Arg Lys Ala Val Asp Arg Pro Ile Glu His Glu Asn Ser Lys
725 730 735
Arg Thr Ala Gly Leu Val Val Asp Leu Thr Gly Gly Glu Arg Ser Gln
740 745 750
Glu Ile Leu Thr Leu Thr Ser Ser Gly Ser Gln Asp Gln Glu Ala Ala
755 760 765
His Leu Ile Gln Glu Asn Glu Lys Leu Arg Ala Gln Cys Ser Glu Cys
770 775 780
Glu Lys Arg Glu Glu Glu Leu His Ser Lys Val Asn Lys Leu Arg Lys
785 790 795 800
Glu Leu Lys Glu Ala Gln Arg Thr Tyr Ala His Met Leu Val Glu Ser
805 810 815
Arg Leu Leu Asp Lys Val Lys Pro Glu Glu Asp Val Tyr Ile
820 825 830
<210> 56
<211> 670
<212> PRT
<213> 菜豆
<400> 56
Met Gly Pro Thr Glu Ile Val Asp Leu Ser Ser Asp Asp Glu Ser Glu
1 5 10 15
Thr Val Cys Leu Lys Ala Val Lys Leu Glu Gly Asp Phe Val Met Asp
20 25 30
Lys Lys Cys Gln Ser His Thr Thr Gly Gln Asp Ser Glu Glu Asn Leu
35 40 45
Ser Ser Asn Gly Pro Ser Thr Gly His Ser Asn Ser Ser Val Leu Glu
50 55 60
Gln Gly Pro Ser Pro Val Asp Asp Thr Cys Ile Ser Tyr Val Ser Pro
65 70 75 80
Ile Gly Ala Ala Pro Leu Cys Arg Gln Phe Trp Lys Ala Gly Asn Tyr
85 90 95
Asp Asp Gly Ile Gly Ser Lys Val Thr Val Gln Asn Ala Lys Asn Tyr
100 105 110
Leu His Val His Pro Met Phe Leu His Ser Asn Ala Thr Ser His Lys
115 120 125
Trp Ala Phe Gly Ala Ile Ala Glu Leu Leu Asp Asn Ala Val Asp Glu
130 135 140
Ile Gln Asn Gly Ala Thr Phe Val Ile Val Asp Lys Thr Ser Asn Pro
145 150 155 160
Arg Asp Gly Ser Pro Ala Leu Leu Ile Gln Asp Asp Gly Gly Gly Met
165 170 175
Asp Pro Glu Ala Met Arg Arg Cys Met Ser Phe Gly Phe Ser Asp Lys
180 185 190
Lys Ser Gln Phe Ala Ile Gly Arg Tyr Gly Asn Gly Phe Lys Thr Ser
195 200 205
Thr Met Arg Leu Gly Ala Asp Val Ile Val Phe Ser Cys His Leu Asn
210 215 220
Asn Arg Ile Leu Thr Gln Ser Ile Gly Leu Leu Ser Tyr Thr Tyr Leu
225 230 235 240
Thr Gln Thr Gln Leu Asp Arg Ile Val Val Pro Met Val Asn Tyr Glu
245 250 255
Phe Asn Thr Ser Thr Gly Ser Leu Glu Val Leu Asn Gly Asn Asp His
260 265 270
Phe Val Ser Asn Leu Ser Leu Leu Leu Arg Trp Ser Pro Tyr Ser Ser
275 280 285
Glu Ala Asp Leu Leu Lys Gln Phe Asp Glu Ile Gly Ser His Gly Thr
290 295 300
Lys Val Ile Ile Tyr Asn Leu Trp Cys Asn Asp Asp Gly Asn Leu Glu
305 310 315 320
Leu Asp Phe Asp Thr Asn Ser Lys Asp Ile Arg Ile Ala Gly Asp Val
325 330 335
Lys Arg Ile Asp Thr Leu Lys Ala Trp Lys Ser Val Asn Glu Asp His
340 345 350
Ile Ala Asn Arg Phe Arg Tyr Ser Leu His Val Tyr Leu Ser Ile Leu
355 360 365
Tyr Leu Lys Ile Pro Glu Arg Phe Gln Ile Ile Leu Arg Gly Gln Val
370 375 380
Val Lys Pro His Asn Ile Ala Asp Asp Leu Lys Tyr Pro Gln Phe Val
385 390 395 400
Lys Tyr Ala Pro Leu Ile Gly Gly Ser Thr Lys Gly Thr Ala Leu Thr
405 410 415
Val Thr Thr Ile Gly Phe Leu Lys Glu Ala Pro Gln Val Asn Ile His
420 425 430
Gly Phe Asn Val Tyr His Lys Asn Arg Leu Ile Leu Pro Phe Trp Gln
435 440 445
Val Val Asn Tyr Leu Asp Ser Arg Gly Arg Gly Val Val Gly Ile Leu
450 455 460
Gln Ala Asp Phe Ile Glu Pro Thr His Asn Lys Gln Asp Phe Glu Arg
465 470 475 480
Thr Ser Leu Phe Gln Lys Leu Glu Val Arg Leu Lys Glu Met Thr Trp
485 490 495
Glu Tyr Trp Asp Thr His Cys Thr Leu Phe Gly Tyr Lys Lys Glu Lys
500 505 510
Arg Lys Phe Thr Pro Gly Val Thr Ser Met Gln Asn Pro Leu Thr Ile
515 520 525
Glu Lys Pro Val Ile Leu Asn Lys Ser Ser Ser Ser Leu Val Asn Ala
530 535 540
Lys Ala Tyr Gly Asn Ser Leu Gln Cys Ser Lys Asn Leu Gln Ser Leu
545 550 555 560
Asp Gln Gly Ser Gln Ser Lys Arg Lys Lys Thr His Glu Leu Val Asp
565 570 575
Leu Met Asn Thr Gln Glu His Ala Ser Thr Asp Asn Val Ala Cys Val
580 585 590
Gly Phe Ser Gln Asn Lys Gln Thr Met Ala Thr Pro Ala Asp Gln Val
595 600 605
Gly Asp Gln Lys Asn Leu His Leu Ile His Gln Asn Lys Gln Leu His
610 615 620
Ala Lys Cys Leu Glu Phe Glu Lys Thr Gly Glu Glu Leu Asn Leu Lys
625 630 635 640
Val Val Lys Leu Lys Ser Lys Ile Gln Glu Ala Gln Asp Glu Tyr Lys
645 650 655
Arg Leu Leu Ala Glu Ile Lys Ser Leu Asp Val Lys Glu Glu
660 665 670
<210> 57
<211> 709
<212> PRT
<213> 葡萄
<400> 57
Met Ser Phe Pro Asp Ile Val Asp Leu Cys Ser Asp Asp Glu His Val
1 5 10 15
Glu Val Asp Val Lys Pro Val Lys Leu Glu Pro Asn Ile Ile Glu Arg
20 25 30
Glu Ile Gln Leu Phe Lys Cys His Glu Ile Leu Gln Ala Gln Tyr Gln
35 40 45
Leu Ser Lys Thr His Cys Arg Thr Gln Glu Ser Glu Glu Asn Arg Asp
50 55 60
Ser Asn Ala Leu Ser Thr Ala Gln Ser Ser Thr Ser Ile Leu Asp Gln
65 70 75 80
Gly Gln Ser Pro Met Asp Asp Thr Ser Leu Ser Ser Thr Ser Pro Ile
85 90 95
Cys Pro Ala Pro Leu Cys Arg Gln Phe Trp Lys Ala Gly Asn Tyr Asp
100 105 110
Asp Glu Leu Gly Ser Lys Val Thr Leu Gln Asn Gly Lys Asn Tyr Leu
115 120 125
His Val His Pro Met Phe Leu His Ser Asn Ala Thr Ser His Lys Trp
130 135 140
Ala Phe Gly Ala Ile Ala Glu Leu Leu Asp Asn Ala Val Asp Glu Ile
145 150 155 160
Gln Asn Gly Ala Thr Phe Val Ile Val Asp Lys Thr Ser Asn Pro Arg
165 170 175
Asp Gly Ser Pro Ala Leu Leu Ile Gln Asp Asp Gly Gly Gly Met Gly
180 185 190
Pro Glu Ala Met Arg Cys Cys Met Ser Phe Gly Phe Ser Asp Lys Lys
195 200 205
Ser Lys Ser Ala Ile Gly Gln Tyr Gly Asn Gly Phe Lys Thr Ser Ser
210 215 220
Met Arg Leu Gly Ala Asp Val Ile Val Phe Ser Arg His Leu Asp Asn
225 230 235 240
Gly Lys Leu Thr Gln Ser Ala Gly Leu Leu Ser Tyr Ser Phe Leu Arg
245 250 255
Gln Thr Gly His Asp Arg Ile Val Val Pro Met Val Asp Tyr Glu Phe
260 265 270
Asn Thr Ser Thr Ser Asn Met Glu Pro Leu His Arg Tyr Gly Lys Lys
275 280 285
His Phe Thr Ser Asn Leu Ser Ile Leu Leu Arg Trp Ser Pro Tyr Ser
290 295 300
Thr Glu Gln Glu Leu Leu Lys Gln Phe Asp Asp Ile Gly Tyr His Gly
305 310 315 320
Thr Lys Val Val Ile Tyr Asn Leu Trp Phe Ser Asp Asp Gly Asn Val
325 330 335
Glu Leu Asp Phe Asp Ser Asp Pro Glu Asp Ile Arg Ile Gly Gly Asp
340 345 350
Ile Lys Gln Val Thr Thr Ile Pro Ala Trp Lys Thr Ile Asn Glu Gln
355 360 365
His Ile Ala Asn Arg Phe His Ile Ser Leu Arg Val Tyr Leu Ser Ile
370 375 380
Leu Tyr Leu Arg Ile Pro Gln Asn Phe Asn Ile Ile Leu Arg Gly Lys
385 390 395 400
Val Val Glu His His Asn Ile Ala Asn Asp Leu Lys Phe Pro Glu Phe
405 410 415
Ile Leu Tyr Arg Pro Gln Thr Gly Gly Leu Val Glu Gly Thr Val Val
420 425 430
Thr Thr Ile Gly Phe Leu Lys Glu Ala Pro Gln Val Asn Ile His Gly
435 440 445
Phe Asn Val Tyr His Lys Asn Arg Leu Ile Leu Pro Phe Trp Gln Val
450 455 460
Val Asn Tyr Ser Asp Ser Arg Gly Arg Gly Val Val Gly Val Leu Glu
465 470 475 480
Ala Asn Phe Ile Glu Pro Ile His Asn Lys Gln Asp Phe Glu Arg Thr
485 490 495
Ser Leu Phe Gln Lys Leu Glu Gly Arg Leu Lys Glu Met Thr Trp Glu
500 505 510
Tyr Trp Asp Tyr His Cys Gly Leu Ile Gly Tyr Gln Val Lys Lys Lys
515 520 525
Pro Arg Thr Leu Val Ser Pro His Ile Ser Ser His Gly Ser Pro His
530 535 540
Ile Ala Met Glu Lys Pro Ile Gly Leu Asn Gln Ser Ser Pro Ala Ile
545 550 555 560
Gly Asn Ile Lys Ala Thr Pro Pro Gly Gly Ala Phe Gln His Pro Ala
565 570 575
Ala Thr Ala Ser His Cys Leu Ala Gly Arg Pro Gly Glu Pro Ile Thr
580 585 590
Asp Ser Gln Ser Arg Ser Gln Gln Gly Ala Ser Leu Lys Arg Thr Glu
595 600 605
His Asp His Pro Val Glu Pro Glu Lys Lys Lys His Ala Gly Ile Lys
610 615 620
Glu Ile Val Thr Asp Thr His Ser Gln Glu Thr Gln Pro Val Asp Ile
625 630 635 640
Phe Ala Asn His Ser Lys Asp Gln Glu Ala Met Thr Leu Met Gln Glu
645 650 655
Asn Lys Lys Leu His Ala Gln Cys Leu Glu Tyr Glu Lys Arg Glu Lys
660 665 670
Glu Leu Ser Val Lys Met Ile Gln Leu Arg Asn Glu Leu Arg Glu Val
675 680 685
His Asn Glu Gln Ala Arg Leu Leu Ala Glu Leu Gln His Leu Asp Ala
690 695 700
Ile Lys Asp Glu Lys
705
<210> 58
<211> 692
<212> PRT
<213> 可可
<400> 58
Met Asn Cys Val Glu Ile Val Asp Leu Ser Ser Asp Asp Glu Leu Gly
1 5 10 15
Glu Val Asp Val Lys Pro Val Lys Leu Glu Pro Gly Thr Val Val Gly
20 25 30
Ser Thr Thr Lys Gln Lys Asn Asn His Arg Phe Gln Pro Val Glu Leu
35 40 45
Val Lys Tyr Lys Thr Gln Ser Gly Lys Gln Ala Ser Glu Glu Asn Lys
50 55 60
Ser Ser Asn Asn Ala Leu Ser Thr Gly Gln Ser Ser Thr Ser Phe Leu
65 70 75 80
Glu Gln Gly Gln Ser Pro Gly Asp Asp Thr Gly Val Ser Ser Thr Ser
85 90 95
Ala Val Ser Pro Ala Pro Val Cys Arg Gln Phe Trp Lys Ala Gly Ser
100 105 110
Tyr Glu Gly Gly Leu Gly Ser Lys Val Thr Leu Gln Asn Ser Lys Asn
115 120 125
Tyr Leu His Val His Pro Leu Phe Leu His Ser Asn Ala Thr Ser His
130 135 140
Lys Trp Ala Phe Gly Ala Ile Ala Glu Leu Leu Asp Asn Ala Ile Asp
145 150 155 160
Glu Ile Gln Asn Gly Ala Thr Phe Val Ile Val Asp Lys Thr Ser Asn
165 170 175
Pro Arg Asp Gly Ser Pro Ala Leu Leu Ile Gln Asp Asp Gly Gly Gly
180 185 190
Met Asp Pro Glu Ala Met Arg Arg Cys Met Ser Phe Gly Phe Ser Asp
195 200 205
Lys Lys Ser Lys Ser Ala Ile Gly Gln Tyr Gly Asn Gly Phe Lys Thr
210 215 220
Ser Ser Met Arg Leu Gly Gly Asp Val Ile Val Phe Ser Arg His Leu
225 230 235 240
Asp Asn Arg Ser Leu Thr Gln Ser Ile Gly Leu Leu Ser Tyr Thr Phe
245 250 255
Leu Thr Arg Thr Gly His Asp Arg Ile Val Val Pro Met Val Asp Tyr
260 265 270
Glu Leu Asn Val Ser Thr Gly Met Leu Asp Val Leu His Gly Arg Glu
275 280 285
His Phe Val Ser Asn Leu Ser Ile Leu Leu Gln Trp Ser Pro Tyr Ser
290 295 300
Thr Glu Ala Glu Leu Leu Lys Gln Phe Asp Asp Ile Gly Ser His Gly
305 310 315 320
Thr Lys Ile Ile Ile Tyr Asn Leu Trp Leu Asn Asn Asp Arg Asp Ser
325 330 335
Glu Leu Asp Phe Asp Ser Asp Pro Lys Asp Ile Arg Ile Ser Gly Asp
340 345 350
Thr Lys Lys Val Asn Thr Ile Pro Ala Trp Lys Ala Val Asn Glu Gln
355 360 365
His Ile Ala Ser Arg Tyr His Ile Ser Leu Arg Val Tyr Leu Ser Ile
370 375 380
Leu Tyr Leu Arg Ile Pro Asp Thr Phe Arg Ile Ile Leu Arg Gly Glu
385 390 395 400
Ile Val Glu His His Asn Ile Ala Asp Asp Leu Lys Phe Ile Glu Tyr
405 410 415
Ile Leu Tyr Arg Pro Gln Ser Phe Gly Ser Val Glu Gly Thr Val Val
420 425 430
Cys Thr Ile Gly Phe Leu Lys Glu Ala Pro Gln Val Asn Ile His Gly
435 440 445
Phe Cys Val Tyr His Lys Asn Arg Leu Ile Leu Pro Phe Trp Gln Val
450 455 460
Val Ser Tyr Ser Asp Ser Arg Gly Arg Gly Val Val Gly Val Leu Glu
465 470 475 480
Ala Asn Phe Val Glu Pro Thr His Asn Lys Gln Asp Phe Glu Arg Thr
485 490 495
Ser Leu Phe Gln Lys Leu Glu Gly Arg Leu Lys Glu Met Thr Trp Glu
500 505 510
Tyr Trp Asp Tyr His Cys Gly Leu Ile Gly Tyr Gln Val Lys Lys Lys
515 520 525
Ser Arg Pro Ala Ala Ser His Val Ser Ser His Phe Thr Gly Gln Ser
530 535 540
Ser Ala Glu Gln Pro Val Leu Leu Asn Gln Thr Ser Leu Asp Ala Asp
545 550 555 560
Arg Ala Lys Thr Gly Cys Thr Asp Val Gly Val Lys Gln Ser Ile Thr
565 570 575
Asn Ser His Gly Arg Ser Met Gln Gly Leu Pro Thr Lys Arg Lys Glu
580 585 590
Pro Gly Asp Leu Ala Asn Leu Glu Lys Val Lys Arg Gln Ala Gly Thr
595 600 605
Ser Ala Asn Thr Val Val Gln Pro Val Asp Thr Thr Ala Asn His Ser
610 615 620
Lys Asp Gln Glu Thr Ile Tyr Leu Met Gln Glu Asn Lys Lys Leu Arg
625 630 635 640
Ala Lys Cys Ser Glu Tyr Glu Lys Arg Arg Glu Glu Leu Asn Ile Lys
645 650 655
Val Glu Lys Leu Arg Ser Glu Val Gly Glu Val Gln Arg Glu Tyr Tyr
660 665 670
Arg Leu Leu Ala Glu Leu Asn Ser Leu Asp Ile Val Lys Asp Glu Asn
675 680 685
Asp Val Tyr Met
690
<210> 59
<211> 664
<212> PRT
<213> 乌拉尔图小麦（Triticum urarte）
<400> 59
Met Arg Ser Gly Gly Ala Ala Glu Val Ile Asp Leu Ser Ser Asp Asp
1 5 10 15
Asp Gly Glu Ser Leu Pro Phe Pro Ser Thr Ser Ala Val Thr Val Ala
20 25 30
Pro Ser Ser Thr Arg Asp Ala Lys Pro Tyr Glu Leu Ala Asp Val Lys
35 40 45
Pro Leu Leu Tyr Pro Ile Gln His Pro Gly Cys Tyr Ala Leu Val Pro
50 55 60
Val Lys Asp Glu Asp Pro Val Pro Leu Pro Leu Ala Ala Lys Ala Pro
65 70 75 80
Arg Val Leu Pro Pro Pro Arg Leu Cys Arg Gln Phe Trp Lys Ser Gly
85 90 95
Glu Tyr Val Val Ala Arg Arg Asn Pro Asp Ala Asp Ala Pro Gly Gly
100 105 110
Arg Asn Arg Leu Arg Ile Asn Pro Lys Phe Leu His Ser Asn Ala Thr
115 120 125
Ser His Lys Trp Ala Phe Gly Val Leu Thr Ser Leu Ile Phe Ala Ala
130 135 140
Ile Ala Glu Leu Leu Asp Asn Ala Ile Asp Glu Val Asn Asn Gly Ala
145 150 155 160
Thr Phe Val Arg Val Asn Lys Phe Thr Asn Pro Arg Asp Gly Ser Pro
165 170 175
Ser Leu Leu Val Gln Asp Asp Gly Gly Gly Met Asp Pro Asp Ala Leu
180 185 190
Arg Cys Cys Met Ser Phe Gly Phe Ser Asp Lys Gln Ser Asp Ala Phe
195 200 205
Ile Gly Gln Tyr Gly Asn Gly Phe Lys Thr Ser Thr Met Arg Leu Gly
210 215 220
Ala Asp Val Ile Val Phe Thr Gln Asn Gln Lys Asn Trp Thr Pro Thr
225 230 235 240
Arg Ser Ile Gly Leu Leu Ser Tyr Thr Phe Leu Met Glu Thr Gly Cys
245 250 255
Asp Asp Val Leu Val Pro Thr Val Asp Tyr Gln Tyr Asp Leu Thr Thr
260 265 270
Ala Ser Tyr Thr Gln Leu Leu Arg His Asn Gln Lys Leu Phe Ser Ser
275 280 285
Asn Leu Ala Ile Leu Ser Lys Trp Ser Pro Phe Ala Ser Glu Ala Glu
290 295 300
Leu Leu Lys Gln Phe Asp Asp Ile Gly Glu His Gly Thr Lys Ile Ile
305 310 315 320
Val Phe Asn Leu Trp Phe Asn Asp Asp Gly Asp Met Glu Leu Asp Phe
325 330 335
Asn Ser Asp Lys Lys Ala Tyr Ala Ser Ile Leu Tyr Leu Arg Val Pro
340 345 350
Asp Ser Phe Arg Ile Ile Leu Arg Gly Gln Asp Val Glu Pro His Asn
355 360 365
Val Val Asn Asp Leu Leu Tyr Arg Glu Cys Val Leu Tyr Lys Pro Gln
370 375 380
Ile Ala Gly Leu Pro Glu Leu Ser Ile Val Thr Thr Ile Gly Phe Val
385 390 395 400
Lys Gly Ala Pro Asp Thr Asp Val Gln Gly Phe Asn Val Tyr His Lys
405 410 415
Asn Arg Leu Ile Thr Pro Phe Trp Lys Val Ala Ser Asn Ser Tyr Gly
420 425 430
Lys Gly Arg Gly Val Val Gly Ile Leu Asp Ala Ser Phe Ile Lys Pro
435 440 445
Thr His Asp Lys Gln Asp Phe Glu Lys Ser Val Leu His Gln Arg Leu
450 455 460
Glu Asn Arg Leu Lys Glu Met Thr Tyr Glu Tyr Trp Gly Leu His Cys
465 470 475 480
His Arg Leu Gly Tyr Asp Asn Lys Ser Leu Pro Lys Ala Ser Arg Ala
485 490 495
Leu Tyr Arg Ala Lys Gln Thr Gly Ala Gly Ser Ser Pro Ala Ser Val
500 505 510
Pro His Gln Leu Leu Thr Ala Asp Ile Pro Thr Ser Ser Cys Val Gln
515 520 525
Ser Ser Met Gly Gln Lys Arg Asn Phe Asp Ala Leu Gly Val Ile Ser
530 535 540
Asp Ile Asn Asn His Gln Thr Lys Ala Arg Glu Thr Ser Ser Met Gly
545 550 555 560
Ile Tyr Leu Phe Cys Leu His Arg Asp Val Ile Gln Arg Lys Arg Phe
565 570 575
His Glu Tyr Lys Thr Leu Thr Leu Glu Asn Asp Lys Leu Arg Asp Glu
580 585 590
Cys Leu Gln Tyr Glu Glu Ser Glu Lys Gln Leu Val Glu Lys Lys Ser
595 600 605
Thr Ile Leu Val Lys Phe Ser His Ile Ile Phe Asn Leu Leu Ile Glu
610 615 620
Ile Met Val Tyr Phe Thr Ile Val Lys Glu Gln Lys Leu Arg Tyr Gln
625 630 635 640
Ile Ala Lys Glu Thr Lys Lys Tyr Glu Glu Leu Leu Glu Glu Leu Lys
645 650 655
Leu Leu Asp Val Lys Leu Glu Thr
660
<210> 60
<211> 688
<212> PRT
<213> 大豆
<400> 60
Met Gly Pro Thr Glu Val Val Tyr Leu Ser Ser Asp Asp Glu Ser Glu
1 5 10 15
Lys Glu Val Ala Phe Lys Ala Val Lys Leu Glu Glu Asp Phe Val Leu
20 25 30
Asp Ala Lys Gln Tyr Asn Glu Thr Asn Lys Gly Gln Leu Ala Lys His
35 40 45
Lys Arg Ser Gln Ser His Thr Thr Gly Gln Asp Ser Glu Glu Asn Leu
50 55 60
Ser Ser Asn Gly Pro Ser Thr Gly His Ser Asn Ser Ser Val Leu Glu
65 70 75 80
Gln Gly Pro Ser Pro Val Asp Asp Thr Gly Ile Ser Tyr Ala Ser Ser
85 90 95
Ile Gly Val Ala Pro Leu Cys Arg Gln Phe Trp Lys Ala Gly Asn Tyr
100 105 110
Asp Asp Gly Leu Gly Ser Lys Val Thr Val Gln Asn Ala Lys Asn Tyr
115 120 125
Leu His Val His Pro Met Phe Leu His Ser Asn Ala Thr Ser His Lys
130 135 140
Trp Ala Phe Gly Ala Ile Ala Glu Leu Leu Asp Asn Ala Val Asp Glu
145 150 155 160
Ile Gln Asn Gly Ala Thr Phe Val Ile Val Asp Lys Thr Ser Asn Pro
165 170 175
Arg Asp Gly Asn Pro Ala Leu Leu Ile Gln Asp Asp Gly Gly Gly Met
180 185 190
Asp Pro Asp Ala Met Arg Arg Cys Met Ser Phe Gly Phe Ser Asp Lys
195 200 205
Lys Ser Gln Phe Ala Ile Gly Arg Tyr Gly Asn Gly Phe Lys Thr Ser
210 215 220
Ser Met Arg Leu Gly Ala Asp Val Ile Val Phe Ser Cys His Leu Asn
225 230 235 240
Asn Arg Ile Leu Thr Gln Ser Ile Gly Leu Leu Ser Tyr Thr Tyr Leu
245 250 255
Ile Lys Thr Gln Leu Asp Arg Ile Val Val Pro Met Val Asn Tyr Glu
260 265 270
Phe Asp Thr Ser Thr Gly Ser Leu Lys Ile Leu Asn Gly Asn Glu His
275 280 285
Phe Val Ser Asn Leu Ser Leu Leu Leu Arg Trp Ser Pro Tyr Ser Ser
290 295 300
Glu Ala Asp Leu Leu Lys Gln Phe Asp Asp Ile Gly Ser His Gly Thr
305 310 315 320
Lys Val Ile Ile Tyr Asn Leu Trp Cys Asn Asp Asp Ala Asn Leu Glu
325 330 335
Leu Asp Phe Asp Thr Asp Pro Thr Asp Ile Arg Ile Ala Gly Asp Val
340 345 350
Lys Gln Ile Asp Thr Leu Lys Ala Trp Lys Ser Val Asn Glu Glu His
355 360 365
Ile Ala Asn Arg Leu Arg Tyr Ser Leu His Val Tyr Met Ser Ile Leu
370 375 380
Tyr Leu Lys Ile Pro Glu Ser Phe Gln Met Ile Leu Arg Gly Gln Val
385 390 395 400
Val Lys Pro His Asn Ile Ala Asp Asp Leu Lys Phe Pro Gln Phe Val
405 410 415
Lys Tyr Ala Pro Val Ile Gly Gly Ser Val Lys Gly Thr Ala Leu Thr
420 425 430
Val Thr Thr Ile Gly Phe Leu Lys Glu Ala Pro Gln Val Asn Ile His
435 440 445
Gly Phe Asn Val Tyr His Lys Asn Arg Leu Ile Leu Pro Phe Trp Gln
450 455 460
Val Val Ser Tyr Leu Asp Ser Arg Gly Arg Gly Val Val Gly Ile Leu
465 470 475 480
Gln Ala Asp Phe Ile Glu Pro Thr His Asn Lys Gln Asp Phe Glu Arg
485 490 495
Thr Ser Leu Phe Gln Lys Leu Glu Gly Arg Leu Lys Glu Met Thr Trp
500 505 510
Glu Tyr Trp Asp Thr His Cys Thr Leu Phe Gly Tyr Lys Asp Lys Asp
515 520 525
Lys Lys Lys Leu Pro Pro Arg Val Thr Ser Met Gln Lys Pro Leu Ala
530 535 540
Ile Glu Lys Pro Val Met Leu Asn Arg Ser Cys Ser Pro Val Val Asn
545 550 555 560
Thr Lys Ile Glu Tyr Gly Asn Ser Glu Gln Cys Ser Thr Lys Leu Gln
565 570 575
Ile Arg Ser Glu Gln Gly Ser His Asn Lys Arg Lys Thr His Glu Leu
580 585 590
Val Asp Leu Gln Asn Thr Glu Lys His Ala Arg Thr Glu Asn Val Thr
595 600 605
Cys Val Gly Phe Ser Gln Asn Lys Gln Ile Ile Ala Thr Pro Ala Asp
610 615 620
Gln Val Phe Asp Arg Lys Thr Met His Leu Val Gln Gln Asn Lys Lys
625 630 635 640
Leu His Ala Lys Cys Leu Glu Phe Glu Lys Thr Gly Glu Glu Leu Asn
645 650 655
Leu Lys Val Thr Met Leu Lys Ser Glu Ile Gln Glu Ala Gln Asp Glu
660 665 670
Tyr Lys Arg Leu Leu Ala Glu Val Lys Ser Leu Asp Leu Lys Glu Glu
675 680 685
<210> 61
<211> 682
<212> PRT
<213> 稻
<220>
<223> 粳型
<400> 61
Met Arg Ser Thr Ala Ala Ala Ala Asp Val Ile Asp Leu Ser Ser Asp
1 5 10 15
Asp Asp Asp Glu Val Pro Val Pro Ser Thr Ser Ala Ala Ala Ala Ala
20 25 30
Ala Ala Arg Arg Val Ala Pro Ser Thr Ser Pro Arg Asp Val Thr Pro
35 40 45
Tyr Ala Leu Val Asp Val Lys Pro Ala Leu Leu Tyr Pro Leu Gln Pro
50 55 60
Pro Gly Val Val Val Gly Gly Ser Gly Ala Leu Val Pro Val Lys Glu
65 70 75 80
Glu Leu Pro Val Leu Thr Pro Val Pro Leu Leu Ala Ala Gly Tyr Ser
85 90 95
Pro Ser Thr Pro Ser Thr Lys Val Ala Leu Pro Ala Pro Arg Leu Cys
100 105 110
Arg Gln Phe Trp Lys Ser Gly Asp Tyr Val Val Ala Gln Arg Asn Pro
115 120 125
Asp Ala Asp Ala Pro Gly Gly Arg Asn Arg Leu Arg Ile Asn Pro Arg
130 135 140
Phe Leu His Ser Asn Ala Thr Ser His Lys Trp Ala Phe Gly Ala Ile
145 150 155 160
Ala Glu Leu Leu Asp Asn Ala Ile Asp Glu Val Asn Thr Gly Ala Thr
165 170 175
Phe Val Arg Val Asn Glu Phe Thr Asn Pro Arg Asp Gly Ser Ser Ser
180 185 190
Leu Leu Ile Gln Asp Asp Gly Gly Gly Met Asp Pro Glu Ala Leu Arg
195 200 205
Arg Cys Met Ser Phe Gly Phe Ser Asp Lys Gln Ser Asp Ala Leu Ile
210 215 220
Gly Gln Tyr Gly Asn Gly Phe Lys Thr Ser Thr Met Arg Leu Gly Ala
225 230 235 240
Asp Val Ile Val Phe Thr Gln Asn Gln Asn Asn Trp Val Pro Thr Arg
245 250 255
Ser Ile Gly Leu Leu Ser Tyr Thr Phe Leu Met Glu Thr Gly Cys Asp
260 265 270
Asp Val Leu Val Pro Thr Val Asp Tyr Gln Tyr Asp Ile Ser Thr Ala
275 280 285
Ser Tyr Thr Gln Met Leu Arg His Asp Gln Lys Leu Phe Ser Ser Asn
290 295 300
Leu Ala Ile Leu Leu Lys Trp Ser Pro Phe Ala Ser Glu Ala Glu Leu
305 310 315 320
Leu Lys Gln Phe Asp Asp Ile Gly Glu His Gly Thr Lys Ile Ile Val
325 330 335
Phe Asn Leu Trp Phe Asn Asp Asp Gly Asp Met Glu Leu Asp Phe Asn
340 345 350
Ser Asp Lys Lys Asp Ile Leu Ile Thr Gly Ala His Arg Lys Val Asn
355 360 365
Thr Asn Lys Ala Asp Lys Val Ala Thr Gln Asn Tyr Val Ser Thr Arg
370 375 380
Leu Arg Tyr Ser Leu Arg Ala Tyr Thr Ser Val Leu Tyr Leu His Ile
385 390 395 400
Pro Asp Asn Phe Arg Ile Val Leu Arg Gly His Asp Val Glu Ser His
405 410 415
Asn Val Ile Asn Asp Leu Met Tyr Pro Glu Cys Val Leu Tyr Lys Pro
420 425 430
Gln Ile Ala Gly Leu Ala Glu Leu Ser Ala Ile Thr Thr Ile Gly Phe
435 440 445
Val Lys Gly Ala Pro Glu Ile Asp Val Gln Gly Phe Asn Val Tyr His
450 455 460
Lys Asn Arg Leu Ile Ala Pro Phe Trp Lys Val Ala Asn Asn Ser Tyr
465 470 475 480
Gly Lys Gly Arg Gly Val Val Gly Ile Leu Glu Ala Asn Phe Ile Lys
485 490 495
Pro Thr His Asp Lys Gln Asp Phe Glu Lys Ser Val Leu Tyr Gln Arg
500 505 510
Leu Glu Ser Arg Leu Lys Glu Met Thr Tyr Glu Tyr Trp Asp Leu His
515 520 525
Cys His Arg Ile Gly Tyr Asp Asn Lys Lys Leu Pro Lys Ser Ser Arg
530 535 540
Ala Leu Asn Arg Ala Asn Gln Met Asn Ala Gly Ser Ser Pro Pro Ile
545 550 555 560
Val Pro Arg Gln Leu Leu Ala Ala Asp Ile Pro Thr Ser Ser Cys Ala
565 570 575
Val Pro Thr Phe Met Ala Pro Ala Leu Arg Gln Lys Gln Met Gly Leu
580 585 590
Lys Arg Asn Ile Asp Ala Leu Gly Ser Lys Thr Asp Ser Ala Asp Gln
595 600 605
Asp Gly Ser His Leu Asp Val Ser Gln Arg Arg Arg Phe Asn Glu Tyr
610 615 620
Arg Thr Leu Thr Leu Glu Asn Asp Lys Leu Arg Gly Glu Cys Leu Gln
625 630 635 640
Tyr Glu Glu Ser Ala Lys Gln Leu Ala Leu Lys Glu Gln Lys Leu Arg
645 650 655
Ser Gln Ile Ala Leu Glu Arg Lys Lys Tyr Glu Glu Leu Leu Gln Glu
660 665 670
Leu Arg Ser Leu Asp Val Lys Thr Glu Lys
675 680
<210> 62
<211> 648
<212> PRT
<213> 稻
<220>
<223> 籼型
<400> 62
Met Arg Ser Thr Ala Ala Ala Ala Asp Val Ile Asp Leu Ser Ser Asp
1 5 10 15
Asp Asp Asp Glu Val Pro Val Pro Ser Thr Ser Ala Ala Ala Ala Ala
20 25 30
Ala Ala Arg Arg Val Ala Pro Ser Thr Ser Pro Arg Asp Val Thr Pro
35 40 45
Tyr Ala Leu Val Asp Val Lys Pro Ala Leu Leu Tyr Pro Leu Gln Pro
50 55 60
Pro Gly Val Val Val Gly Gly Ser Gly Ala Leu Val Pro Val Lys Glu
65 70 75 80
Glu Leu Pro Leu Leu Thr Pro Val Pro Leu Leu Ala Ala Gly Tyr Ser
85 90 95
Pro Ser Thr Pro Ser Thr Lys Val Ala Leu Pro Ala Pro Arg Leu Cys
100 105 110
Arg Gln Phe Trp Lys Ser Gly Asp Tyr Val Val Ala Gln Arg Asn Pro
115 120 125
Asp Ala Asp Ala Pro Gly Gly Arg Asn Arg Leu Arg Ile Asn Pro Arg
130 135 140
Phe Leu His Ser Asn Ala Thr Ser His Lys Trp Ala Phe Gly Ala Ile
145 150 155 160
Ala Glu Leu Leu Asp Asn Ala Ile Asp Glu Val Asn Thr Gly Ala Thr
165 170 175
Phe Val Arg Val Asn Glu Phe Thr Asn Pro Arg Asp Gly Ser Ser Ser
180 185 190
Leu Leu Ile Gln Asp Asp Gly Gly Gly Met Asp Pro Glu Ala Leu Arg
195 200 205
Arg Cys Met Ser Phe Gly Phe Ser Asp Lys Gln Ser Asp Ala Leu Ile
210 215 220
Gly Gln Tyr Gly Asn Gly Phe Lys Thr Ser Thr Met Arg Leu Gly Ala
225 230 235 240
Asp Val Ile Val Phe Thr Gln Asn Gln Asn Asn Trp Val Pro Thr Arg
245 250 255
Ser Ile Gly Leu Leu Ser Tyr Thr Phe Leu Met Glu Thr Gly Cys Asp
260 265 270
Asp Val Leu Val Pro Thr Val Asp Tyr Gln Tyr Asp Ile Ser Thr Ala
275 280 285
Ser Tyr Thr Gln Met Leu Arg His Asp Gln Lys Leu Phe Ser Ser Asn
290 295 300
Leu Ala Ile Leu Leu Lys Trp Ser Pro Phe Ala Ser Glu Ala Glu Leu
305 310 315 320
Leu Lys Gln Phe Asp Asp Ile Gly Glu His Gly Thr Lys Ile Ile Val
325 330 335
Phe Asn Leu Trp Phe Asn Asp Asp Gly Asp Met Glu Leu Asp Phe Asn
340 345 350
Ser Asp Lys Lys Ala Tyr Thr Ser Val Leu Tyr Leu His Ile Pro Asp
355 360 365
Asn Phe Arg Ile Val Leu Arg Gly His Asp Val Glu Ser His Asn Val
370 375 380
Ile Asn Asp Leu Met Tyr Pro Glu Cys Val Leu Tyr Lys Pro Gln Ile
385 390 395 400
Ala Gly Leu Ala Glu Leu Ser Ala Ile Thr Thr Ile Gly Phe Val Lys
405 410 415
Gly Ala Pro Glu Ile Asp Val Gln Gly Phe Asn Val Tyr His Lys Asn
420 425 430
Arg Leu Ile Ala Pro Phe Trp Lys Val Ala Asn Asn Ser Tyr Gly Lys
435 440 445
Gly Arg Gly Val Val Gly Ile Leu Glu Ala Asn Phe Ile Lys Pro Thr
450 455 460
His Asp Lys Gln Asp Phe Glu Lys Ser Val Leu Tyr Gln Arg Leu Glu
465 470 475 480
Ser Arg Leu Lys Glu Met Thr Tyr Glu Tyr Trp Asp Leu His Cys His
485 490 495
Arg Ile Gly Tyr Asp Asn Lys Lys Leu Pro Lys Ser Ser Arg Ala Leu
500 505 510
Asn Arg Ala Asn Gln Met Asn Ala Gly Ser Ser Pro Pro Ile Val Pro
515 520 525
Arg Gln Leu Leu Ala Ala Asp Ile Pro Thr Ser Ser Cys Ala Val Pro
530 535 540
Thr Phe Met Ala Pro Ala Leu Arg Gln Lys Gln Met Gly Leu Lys Arg
545 550 555 560
Asn Ile Asp Ala Leu Gly Ser Lys Thr Asp Ser Ala Asp Gln Asp Gly
565 570 575
Ser His Leu Asp Val Ser Gln Arg Arg Arg Phe Asn Glu Tyr Arg Thr
580 585 590
Leu Thr Leu Glu Asn Asp Lys Leu Arg Gly Glu Cys Leu Gln Tyr Glu
595 600 605
Glu Ser Ala Lys Gln Leu Ala Leu Lys Glu Gln Lys Leu Arg Ser Gln
610 615 620
Ile Ala Leu Glu Arg Lys Lys Tyr Glu Glu Leu Leu Gln Glu Leu Arg
625 630 635 640
Ser Leu Asp Val Lys Thr Glu Lys
645
<210> 63
<211> 750
<212> PRT
<213> 玉蜀黍
<400> 63
Met Cys Met Ser Ser Leu Ile Ala Pro Thr Arg Pro Arg Arg Leu Ser
1 5 10 15
Phe Glu Asn Ser Lys Arg Leu Glu Glu Ala Ser Thr Asp Tyr Met Asp
20 25 30
Ala Glu Arg Asp Ile Lys Pro Phe Val Phe Pro Thr Val Pro Thr Thr
35 40 45
Ile Gln Arg Asn Phe His Gly Pro Gly Ala Arg Gln Pro Thr Ala Arg
50 55 60
Pro Leu Glu Arg Cys Gly Thr Arg Val Leu Pro Gln Thr His Arg Pro
65 70 75 80
Cys Ser Ala Pro Arg Leu Ser Arg Lys Phe Trp Gly Ala Gly Asp Tyr
85 90 95
Asp Ala Val Ala Gly Arg Ser Ala Pro Gln Pro Pro Ser Leu Gln Asn
100 105 110
Arg Met Cys Val His Pro Glu Phe Leu His Ser Asn Ala Thr Ser His
115 120 125
Lys Trp Pro Phe Gly Val Cys Leu Thr Phe Phe Pro Ile Ala Ile Ala
130 135 140
Val Ala Glu Leu Leu Asp Asn Ala Val Asp Glu Ile Glu Thr Gly Gly
145 150 155 160
Ala Thr Thr Ile Leu Leu Asp Lys Leu Ile Asp Lys Arg Asn Gly Ser
165 170 175
Pro Ala Leu Leu Ile Gln Asp Asp Gly Gly Gly Met Asp Pro Asp Ser
180 185 190
Leu Arg Arg Cys Met Ser Phe Gly Phe Ser Glu Lys Gln Ser Gly Ser
195 200 205
Ser Ile Gly Gln Tyr Gly Asn Gly Phe Lys Thr Ser Thr Met Arg Leu
210 215 220
Gly Ala Asp Ala Ile Val Phe Ser Arg Cys Cys Thr Arg Ser Ser Gly
225 230 235 240
Pro Thr Gln Ser Ile Gly Leu Leu Ser Tyr Thr Phe Leu Val Glu Thr
245 250 255
Gly His Thr Asn Val Val Val Pro Met Arg Leu Glu Arg His Gly Ser
260 265 270
Lys Gln Phe Phe Ser Asn Leu Ser Ala Leu Leu Lys Trp Ser Pro Phe
275 280 285
Ala Thr Glu Glu Glu Leu Met Gln Asn Phe Cys Asp Ile Gly Pro His
290 295 300
Gly Thr Lys Ile Ile Val Phe Asn Leu Trp Ser Asn Asp Asp Gly Asn
305 310 315 320
Leu Glu Leu Asp Phe Asp Thr Asn Pro Glu Asp Ile Met Ile Ser Gly
325 330 335
Ala Pro Asn Pro Glu Gln Ile Ser Asn Ser Val Arg Arg Ala Asn Glu
340 345 350
Asn His Leu Ala Asn Arg Leu Arg Tyr Ser Leu Arg Val Tyr Ala Ser
355 360 365
Val Leu Tyr Leu Gln Leu Pro Asp Tyr Phe Arg Ile Met Leu Arg Gly
370 375 380
Arg Glu Val Glu Arg His His Ile Ala Ser Asp Leu Ile Tyr Pro Glu
385 390 395 400
Arg Ile Ser Tyr Arg Pro Gln Ser Cys Gly Ile Ile Arg Glu Ala Glu
405 410 415
Val Leu Thr Thr Ile Gly Phe Leu Lys Gly Ala Pro Thr Ile Ser Val
420 425 430
His Gly Phe Asn Ile Tyr His Lys Asn Arg Leu Ile Leu Pro Phe His
435 440 445
Arg Val Leu Ser Thr Ala Ser Ser Lys Gly Arg Ser Val Ser Gly Val
450 455 460
Leu Glu Val Asp Phe Ile Lys Pro Thr His Asp Lys Gln Asp Phe Glu
465 470 475 480
Lys Ser Gln Leu Phe Gln Arg Leu Met Asn Arg Leu Lys Glu Met Thr
485 490 495
Asn Glu Tyr Cys Thr Asp Ala Tyr Phe Ala Ser Asn Val Thr Thr Gln
500 505 510
Met Ser Arg Ser Phe His Arg Asp Leu Tyr Ser Asn Lys Ile Gly Tyr
515 520 525
Val Lys Val Pro Arg Val Arg Ala Ala Pro Thr Pro Ala Pro Pro Val
530 535 540
Met Leu Ala Ile Glu Asn Gly Pro Ala Glu Pro Ser Glu Arg Ser Ala
545 550 555 560
Pro Ala Pro Thr Gln Pro Leu Arg Trp Gln His Gly Asn Ser Ser Cys
565 570 575
Val Asn Ala Val Pro Ile Ala Met Ala Pro Pro Ala Phe His Pro Ala
580 585 590
Pro Val Lys Thr Glu Thr Asp Ala Pro Gly Ala Pro Thr Gly Tyr Ala
595 600 605
Tyr Ser Tyr Ser Pro Ser Trp Pro Ser Val Gln Ala Met Gln Ile Glu
610 615 620
Lys Ile Lys Glu Glu Pro Ser Met Ala Ala Pro Gly Thr Asp Trp Val
625 630 635 640
Glu Thr Arg Lys Arg Thr Asn Asp Asp Gly Ala Thr Thr Val Ala Leu
645 650 655
Phe Lys Arg Arg Lys Ala Pro Gln Glu Leu Ala Gly Ser Gly Ser Arg
660 665 670
Ser Ala Thr Asp Gln Val Ser Arg Tyr Val Gly Glu Gly Glu Leu Asn
675 680 685
Glu Phe Cys Phe Leu Lys Met Glu Asn Arg Met Leu Arg Gly Glu Cys
690 695 700
Ser Glu Phe Glu Met Ala Glu Lys Glu Leu Arg Val Lys Glu Gln Asn
705 710 715 720
Leu Arg Leu Glu Ile Glu Met Ala Gln Glu Gln Phe Lys Arg Leu Leu
725 730 735
Gly Glu Tyr Val Ser Phe Ser Ser Met Pro Thr Arg Lys Pro
740 745 750
<210> 64
<211> 676
<212> PRT
<213> 两色蜀黍
<400> 64
Met Ser Leu Gly Ala Glu Val Ile Glu Leu Ser Ser Asp Glu Glu Glu
1 5 10 15
Glu Pro Val Pro Ser Thr Thr Ala Leu Ala Arg His Ala Pro Ser Ser
20 25 30
Pro Pro Asp Val Lys Pro His Leu Phe Ala Asp Val Asp Val Lys Pro
35 40 45
Leu Leu Leu Pro Pro Pro Leu His Pro Pro Gly Tyr Gly Ala Leu Val
50 55 60
Pro Val Lys Thr Glu Glu Pro Val Pro Val Pro Val Ala Thr Val Ser
65 70 75 80
Pro Pro Pro Arg Ala Leu Pro Pro Pro Arg Leu Cys Arg Gln Phe Trp
85 90 95
Lys Ser Gly Asp Tyr Val Val Ala Arg Arg Asn Pro Asp Ala Asp Val
100 105 110
Pro Gly Gly Arg Asn Arg Leu Arg Ile Asn Pro Lys Phe Leu His Ser
115 120 125
Asn Ala Thr Ser His Lys Trp Ala Phe Gly Ala Ile Ala Glu Leu Leu
130 135 140
Asp Asn Ala Ile Asp Glu Val Asn Asn Gly Ala Thr Phe Val Arg Val
145 150 155 160
Asn Lys Phe Lys Asn Pro Arg Asp Gly Asn Pro Ser Leu Leu Val Gln
165 170 175
Asp Asp Gly Gly Gly Met Asp Pro Glu Ala Leu Arg Arg Cys Met Ser
180 185 190
Phe Gly Phe Ser Asp Lys Gln Ser Asp Ala Phe Ile Gly Gln Tyr Gly
195 200 205
Asn Gly Phe Lys Thr Ser Thr Met Arg Leu Gly Ala Asp Val Ile Val
210 215 220
Phe Thr Gln Asn Gln Asn Asn Trp Val Pro Thr Arg Ser Ile Gly Leu
225 230 235 240
Leu Ser Tyr Thr Phe Leu Met Glu Thr Gly Cys Asp Asp Val Leu Val
245 250 255
Pro Thr Val Asp Tyr Gln Tyr Asp Pro Thr Thr Thr Ser Tyr Val Gln
260 265 270
Met Leu Arg His Asp Gln Lys Leu Phe Ser Ser Asn Leu Ala Ile Leu
275 280 285
Leu Lys Trp Ser Pro Phe Ala Ser Glu Ala Glu Leu Leu Lys Gln Phe
290 295 300
Asp Asp Met Gly Asp His Gly Thr Lys Ile Ile Val Phe Asn Leu Trp
305 310 315 320
Phe Asn Asp Asp Gly Asp Met Glu Leu Asp Phe Asn Ser Asp Lys Lys
325 330 335
Asp Ile Leu Ile Thr Gly Ala His Lys Met Val Lys Thr Asn Asn Pro
340 345 350
Glu Lys Ile Ala Ala Gln Lys Tyr Val Ser Thr Arg Leu Arg Phe Ser
355 360 365
Leu Arg Ala Tyr Ala Ser Ile Leu Tyr Leu His Val Pro Asp Thr Phe
370 375 380
Arg Ile Ile Leu Arg Gly Arg Asp Val Glu Pro His Asn Ile Val Asn
385 390 395 400
Asp Leu Met Tyr Arg Glu Cys Val Leu Tyr Lys Pro Gln Leu Ala Gly
405 410 415
Leu Thr Glu Ser Ser Val Ile Thr Thr Ile Gly Phe Val Lys Gly Ala
420 425 430
Pro Asp Ile Asp Val Gln Gly Phe Asn Val Tyr His Lys Asn Arg Leu
435 440 445
Ile Ser Pro Phe Trp Lys Val Ala Asn Asn Ser Tyr Gly Lys Gly Arg
450 455 460
Gly Val Val Gly Ile Leu Glu Ala Asn Phe Ile Lys Pro Thr His Asp
465 470 475 480
Lys Gln Asp Phe Glu Lys Ser Val Leu Tyr Gln Arg Leu Glu Ile Arg
485 490 495
Leu Lys Glu Met Thr Tyr Glu Tyr Trp Asp Leu His Cys His Arg Val
500 505 510
Gly Tyr Asp Asn Lys Lys Leu Pro Lys Ala Ile Arg Ala Val Asn Arg
515 520 525
Ala Asn His Met Asn Gly Gly Ser Ser Pro Ile Ser Ala Pro Pro Arg
530 535 540
Leu Leu Ala Ala Asp Ile Pro Thr Ser Ser Cys Gly Ile Pro Arg Leu
545 550 555 560
Ser Ala Ser Ala Ala Arg Glu Lys Ile Asn Ser Leu Asp Ser Leu Ser
565 570 575
Lys Ser Pro Met Gly Leu Lys Arg Lys Phe Asn Ser Phe Ser Ala Met
580 585 590
Ala Gly Ser Ala Asp Gln Asp Gly Leu Asp His Met Asp Lys Val Asp
595 600 605
Val Ser Gln Arg Lys Arg Phe Asn Glu Tyr Lys Thr Leu Thr Leu Glu
610 615 620
Asn Asp Lys Leu Arg Asn Glu Cys Leu Gln Tyr Glu Glu Ser Glu Lys
625 630 635 640
Gln Leu Val Leu Lys Glu Gln Lys Leu Arg Ser Glu Ile Ala Glu Ala
645 650 655
Glu Lys Lys Tyr Gln Glu Leu Leu Val Glu Leu Arg Ser Leu Asp Val
660 665 670
Lys Lys Glu Lys
675
<210> 65
<211> 914
<212> PRT
<213> 人工序列
<220>
<223> 合成的构建体
<400> 65
Met Ser His Asp Arg Ser Val Asn Val Ser His Asp Ala Val Ile Ala
1 5 10 15
Lys Pro Glu Arg Gly Thr Met Leu Gln Ser Phe Ser Pro Arg Ser His
20 25 30
Gly Ser Lys Gly Tyr Ser Leu Pro Gln Asp Ser Glu Glu Asn Arg Gly
35 40 45
Ser Val Gly Gln Ser Ala Gly Gln Ser Ser Thr Ser Val Val Asp Gln
50 55 60
Val Arg Ser Pro Ala Asp Asp Ala Gly Val Thr Ser Ser Ser Thr Ile
65 70 75 80
Cys Pro Ala Pro Val Cys Arg Gln Phe Trp Lys Ala Gly Ser Tyr Asn
85 90 95
Asp Glu Leu Ser Ser Lys Ser Gln Gln Pro Asn Gly Lys Asn Tyr Leu
100 105 110
His Val His Pro Met Phe Leu His Ser Asn Ala Thr Ser His Lys Trp
115 120 125
Ala Phe Gly Ala Val Ala Glu Leu Leu Asp Asn Ala Val Asp Glu Ile
130 135 140
Gln Asn Gly Ala Thr Phe Val Ile Val Asp Lys Thr Thr Asn Pro Arg
145 150 155 160
Asp Gly Ala Thr Ala Leu Leu Ile Gln Asp Asp Gly Gly Gly Met Asp
165 170 175
Pro Gln Ala Met Arg His Cys Met Gly Phe Gly Phe Ser Asp Lys Lys
180 185 190
Ser Asp Ser Ala Ile Gly Arg Tyr Gly Asn Gly Phe Lys Thr Ser Thr
195 200 205
Met Arg Leu Gly Ala Asp Val Ile Val Phe Ser Arg His Ser Lys Asn
210 215 220
Gln Thr Leu Thr Gln Ser Ile Gly Leu Leu Ser Tyr Thr Tyr Leu Thr
225 230 235 240
Arg Thr Gly His Asp Arg Ile Val Val Pro Ile Leu Asp Tyr Glu Phe
245 250 255
Asn Ala Ser Ala Gly Glu Phe Lys Thr Leu Gln Asp Arg Glu His Phe
260 265 270
Ile Ser Ser Leu Ser Ile Leu Leu Glu Trp Ser Pro Phe Ser Thr Glu
275 280 285
Ala Glu Leu Leu Gln Gln Phe Asp Asp Val Gly Pro His Gly Thr Lys
290 295 300
Val Ile Ile Tyr Asn Met Trp Leu Asn Ser Asp Ala Lys Leu Glu Leu
305 310 315 320
Asp Phe Asp Ser Val Ala Glu Asp Ile Leu Ile Glu Gly Ser Ile Lys
325 330 335
Lys Thr Gly Ser Lys Ile Val Asn Asp His Ile Ala Ser Arg Phe Ser
340 345 350
Tyr Ser Leu Arg Val Tyr Leu Ser Ile Leu Tyr Leu Arg Ile Pro Glu
355 360 365
Thr Phe Lys Ile Ile Leu Arg Gly Lys Val Val Glu His His Asn Val
370 375 380
Ala Asp Asp Leu Met His Pro Gln Tyr Ile Leu Tyr Lys Pro Gln Ala
385 390 395 400
Ala Gly Ser Glu Glu Ala Leu Val Val Thr Thr Ile Gly Phe Leu Lys
405 410 415
Glu Ala Pro Lys Val Asn Leu His Gly Phe Cys Val Tyr His Lys Asn
420 425 430
Arg Leu Ile Met Pro Phe Trp Gln Val Ile Asn Tyr Ser Ser Ser Arg
435 440 445
Gly Arg Gly Val Val Gly Val Leu Glu Ala Asn Phe Val Glu Pro Thr
450 455 460
His Asn Lys Gln Asp Phe Glu Lys Thr Val Leu Leu Gln Lys Leu Glu
465 470 475 480
Asn Arg Leu Lys Glu Met Thr Val Glu Tyr Trp Ser Cys His Cys Val
485 490 495
Leu Ile Gly Tyr Gln Val Asn Lys Lys Pro Arg Leu Gln Ile Pro Gln
500 505 510
Lys Val Gln Pro Ala Gly Arg Gln Ala Leu Ser Pro Pro Pro Gly Phe
515 520 525
Gln Ala Val Phe Pro Gln Gly Asn Thr Thr Ser Leu Pro Arg Val Ser
530 535 540
Thr Gln Pro Val Leu Leu Glu Lys Arg Lys Glu His Pro Asp Ser Val
545 550 555 560
Ala Ser Ala Ala Leu Lys Arg Lys Val Gly Asn Asp Asp Phe Thr Val
565 570 575
Pro Gly His Ile Arg Val Glu Gln Phe Ile His Gly Ser Ala Ser Gln
580 585 590
Ser Gln Asp Ile Glu Thr Val Lys Leu Met Glu Glu Asn Lys Lys Leu
595 600 605
Arg Ala Lys Cys Leu Asp Arg Lys Val Arg Ser Gln Asn Leu Glu Val
610 615 620
Lys Ala Met Asn Leu Arg Ser Glu Leu Glu Asn Tyr Lys Ser Glu Tyr
625 630 635 640
Glu Arg Leu Met Val Glu Leu Gln Ala Leu Asp Leu Val Lys Asp Glu
645 650 655
His Arg Arg Asn Val Asn Thr Lys Gly Gly Arg Ala Gly Thr Glu Met
660 665 670
Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Gly Met Gly Tyr Pro Tyr
675 680 685
Asp Val Pro Asp Tyr Ala Gly Met Gly Tyr Pro Tyr Asp Val Pro Asp
690 695 700
Tyr Ala Gly Ser Phe Gly Ser Leu Glu Val Leu Phe Gln Gly Pro Leu
705 710 715 720
Glu Pro Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe Ser Gln Ser Gly
725 730 735
Asp Leu Arg Arg His Gln Arg Thr His Thr Gly Glu Lys Pro Tyr Lys
740 745 750
Cys Pro Glu Cys Gly Lys Ser Phe Ser Arg Ser Asp His Leu Thr Thr
755 760 765
His Gln Arg Thr His Thr Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys
770 775 780
Gly Lys Ser Phe Ser Gln Ser Ser Ser Leu Val Arg His Gln Arg Thr
785 790 795 800
His Thr Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe
805 810 815
Ser Thr Ser Gly Asn Leu Val Arg His Gln Arg Thr His Thr Gly Glu
820 825 830
Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe Ser Gln Arg Ala
835 840 845
Asn Leu Arg Ala His Gln Arg Thr His Thr Gly Glu Lys Pro Tyr Lys
850 855 860
Cys Pro Glu Cys Gly Lys Ser Phe Ser Arg Ser Asp Lys Leu Thr Glu
865 870 875 880
His Gln Arg Thr His Thr Gly Leu Glu Gly Ser Met Ala Gly Gly Leu
885 890 895
Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu Asp Thr Gly
900 905 910
Gly Ser
<210> 66
<211> 740
<212> PRT
<213> 拟南芥
<400> 66
Met Arg Ser Tyr Asn Leu Phe His Glu Ser Asn Pro Thr Ile Ser Phe
1 5 10 15
Ser Phe Val Gly Ala Glu Thr Met Ala Pro Asn Leu His Ile Lys Lys
20 25 30
Ala Phe Met Ala Met Arg Ala Met Gly Ile Glu Asp Ala Arg Val Lys
35 40 45
Pro Val Leu Lys Asn Leu Leu Ala Leu Tyr Glu Lys Asn Trp Glu Leu
50 55 60
Ile Ala Glu Asp Asn Tyr Arg Val Leu Ala Asp Ala Ile Phe Asp Ser
65 70 75 80
His Glu Asp Gln Ala Ile Gln Glu Ser Glu Glu Lys Lys Ala Asp Glu
85 90 95
Val Lys Glu Asp Glu Gly Cys Ala Ala Glu Val Asp Arg Gly Lys Lys
100 105 110
Lys Leu His Glu Ser Ile Glu Asp Asp Glu Asp Val Met Ala Glu Ser
115 120 125
Asp Arg Pro Leu Lys Arg Leu Arg Arg Arg Gly Glu Gly Gly Ser Ala
130 135 140
Leu Ala Ser Pro Ser Leu Gly Ser Pro Thr Leu Glu Gly Pro Ser Ile
145 150 155 160
Asn Asp Glu Glu Asn Ala Pro Ile Leu Leu Pro Tyr His Pro Val Pro
165 170 175
Ile Glu Asn Asp His Asp Ala Gly Glu Leu Ile Leu Thr Lys Val Glu
180 185 190
Pro Ile Thr Asn Met Pro Leu Ser Ser Ile Pro Asp Ser Val Asp Arg
195 200 205
Gly Asp Ser Ser Met Leu Glu Ile Asp Lys Ser Asn Gly His Val Glu
210 215 220
Glu Lys Ala Gly Glu Thr Val Ser Thr Ala Asp Gly Thr Thr Asn Asp
225 230 235 240
Ile Ser Pro Thr Thr Val Ala Arg Phe Ser Asp His Lys Leu Ala Ala
245 250 255
Thr Ile Glu Glu Pro Pro Ala Leu Glu Leu Ala Ser Ser Ala Ser Gly
260 265 270
Glu Val Lys Ile Asn Leu Ser Phe Ala Pro Ala Thr Gly Gly Ser Asn
275 280 285
Pro His Leu Pro Ser Met Glu Glu Leu Arg Arg Ala Met Glu Glu Lys
290 295 300
Cys Leu Arg Ser Tyr Lys Ile Leu Asp Pro Asn Phe Ser Val Leu Gly
305 310 315 320
Phe Met Asn Asp Ile Cys Ser Cys Tyr Leu Asp Leu Ala Thr Asn Gly
325 330 335
Arg Asp Ser Ala Asn Gln Leu Pro Lys Asn Leu Pro Phe Val Thr Thr
340 345 350
Asn Ile Asp Ala Leu Lys Lys Ser Ala Ala Arg Met Ala Tyr Thr Ser
355 360 365
Gln Ala Ser Asn Asp Val Val Glu Ile Cys Ser Asn Glu His Met Arg
370 375 380
Asp Ala Glu Asn Gly Ala Val Gly Asp Ser Met Ala Leu Val Val Val
385 390 395 400
Pro Glu Cys Gln Leu Ser Ala Asp Glu Trp Arg Leu Ile Ser Ser Val
405 410 415
Gly Asp Ile Ser Leu Gly Lys Glu Thr Val Glu Ile Pro Trp Val Asn
420 425 430
Glu Val Asn Asp Lys Val Pro Pro Val Phe His Tyr Ile Ala Gln Ser
435 440 445
Leu Val Tyr Gln Asp Ala Ala Val Lys Phe Ser Leu Gly Asn Ile Arg
450 455 460
Asp Asp Gln Cys Cys Ser Ser Cys Cys Gly Asp Cys Leu Ala Pro Ser
465 470 475 480
Met Ala Cys Arg Cys Ala Thr Ala Phe Asn Gly Phe Ala Tyr Thr Val
485 490 495
Asp Gly Leu Leu Gln Glu Asp Phe Leu Glu Gln Cys Ile Ser Glu Ala
500 505 510
Arg Asp Pro Arg Lys Gln Met Leu Leu Tyr Cys Lys Glu Cys Pro Leu
515 520 525
Glu Lys Ala Lys Lys Glu Val Ile Leu Glu Pro Cys Lys Gly His Leu
530 535 540
Lys Arg Lys Ala Ile Lys Glu Cys Trp Ser Lys Cys Gly Cys Met Lys
545 550 555 560
Asn Cys Gly Asn Arg Val Val Gln Gln Gly Ile His Asn Lys Leu Gln
565 570 575
Val Phe Phe Thr Pro Asn Gly Arg Gly Trp Gly Leu Arg Thr Leu Glu
580 585 590
Lys Leu Pro Lys Gly Ala Phe Val Cys Glu Leu Ala Gly Glu Ile Leu
595 600 605
Thr Ile Pro Glu Leu Phe Gln Arg Ile Ser Asp Arg Pro Thr Ser Pro
610 615 620
Val Ile Leu Asp Ala Tyr Trp Gly Ser Glu Asp Ile Ser Gly Asp Asp
625 630 635 640
Lys Ala Leu Ser Leu Glu Gly Thr His Tyr Gly Asn Ile Ser Arg Phe
645 650 655
Ile Asn His Arg Cys Leu Asp Ala Asn Leu Ile Glu Ile Pro Val His
660 665 670
Ala Glu Thr Thr Asp Ser His Tyr Tyr His Leu Ala Phe Phe Thr Thr
675 680 685
Arg Glu Ile Asp Ala Met Glu Glu Leu Thr Trp Asp Tyr Gly Val Pro
690 695 700
Phe Asn Gln Asp Val Phe Pro Thr Ser Pro Phe His Cys Gln Cys Gly
705 710 715 720
Ser Asp Phe Cys Arg Val Arg Lys Gln Ile Ser Lys Gly Lys Asn Val
725 730 735
Lys Lys Arg Ala
740
<210> 67
<211> 783
<212> PRT
<213> 番茄
<400> 67
Met Ala Pro Asn Ser Lys Ser Arg Val Thr Lys Ala Phe Glu Ala Met
1 5 10 15
Lys Val Phe Gly Tyr Ser Glu Thr Val Val Lys Pro Val Leu Arg Asn
20 25 30
Leu Leu Asn Leu Tyr Asn Lys Asn Trp Lys Leu Ile Glu Asp Glu Asn
35 40 45
Tyr Ser Val Leu Leu Glu Ser Ile Ile Asp Ser Glu Glu Ser Lys Glu
50 55 60
Lys Gln Lys Ser Ser Met Glu Asp Glu Pro Glu Glu Asn Glu Pro Pro
65 70 75 80
Leu Lys Arg Ser Arg Leu Tyr Ser Gln Gly Asn His Ser Ser Ala Ala
85 90 95
Lys His Asp Ala Gly Pro Ser Val Asp Thr Cys Thr Ser Glu Leu Gln
100 105 110
Pro Tyr Gly Lys Gln Lys Met Ala Asp Ile Thr Thr Glu Ser Cys Glu
115 120 125
Thr Gln Asp Val Glu Met Lys Pro Arg Phe Leu Leu Asn His His Gln
130 135 140
Arg Lys Gly Lys Lys Gln Ile Ser Ser Glu Ala Ser Pro Val Ser Glu
145 150 155 160
Glu Asp Asn Asp Ile Val Val Leu Ser Asp Asp Asp Lys Gln Glu Thr
165 170 175
Arg Ile Leu Ser Ser His Leu Lys Leu Lys Lys Arg Gly Asp Thr Ser
180 185 190
Arg Leu Tyr Ser Ala Val Lys Pro Lys Arg Arg Leu Ala Tyr Ser Ser
195 200 205
Ser Leu Glu Glu Pro Asn Val Met Gly Ser Thr Asp Val Ser Lys Glu
210 215 220
Gly Ala Leu Val Glu Tyr Ser Phe Ser Asp Ala Met Pro Leu Ser Asp
225 230 235 240
Thr Leu Pro Gly Phe Asp Val Pro Leu Ala Val Val Pro Ser Asp Leu
245 250 255
Glu Arg Leu Asn Ser Glu His Leu Gly Thr Glu Gly Asn Lys Glu Asp
260 265 270
Ala Thr Asn Ser Ser Arg Leu Asn Ile Ala Ser Thr Pro Asn Gly Glu
275 280 285
Val Lys Leu Ser Phe Val Tyr Lys Ile Tyr Ser Ser Ser Asp Phe Cys
290 295 300
Pro Pro Ser Leu Asp Ala Val Phe Lys Arg Met Glu Glu Lys Tyr Met
305 310 315 320
Lys Ser Tyr Arg Phe Ser Gln Pro Gly Phe Leu Leu Ser Leu Met Glu
325 330 335
Asn Leu Cys Lys Cys Tyr Leu Thr Ala Gly Thr Arg Thr Arg Thr Ala
340 345 350
Asn Glu Pro Ser Ala Gly Ile Trp Ser Gln Lys Leu His Pro Val Gly
355 360 365
Val Arg Tyr Asp Ala Thr Asn His Glu Leu His Phe Ala Pro Asp Thr
370 375 380
Ser Asn Gly Ser Phe Lys Leu Ser Asn Leu Ile Lys Ile Leu Pro Gln
385 390 395 400
Ile Pro Thr Phe Thr Ala Ser Gly Asn Arg Asp Ile Met Cys Tyr Met
405 410 415
Val Asp Phe Asn Gly Thr Arg Ile Asn Gly Ala Glu Lys Asp Asn Thr
420 425 430
Asn Lys Leu Leu Lys Leu Leu Ala Ser Ser Thr Met Asn Asn Ser Val
435 440 445
Leu Val Gln Ser Glu His Ser Ser Pro Gly Leu Arg Asn Ser Val Tyr
450 455 460
Tyr Ile Glu Asp Ile Ser Asn Gly Gln Glu Glu His Lys Ile Ser Leu
465 470 475 480
Ile Asn Ala Phe Ser His Val Leu Pro Val Phe Lys Tyr Ile Pro Lys
485 490 495
Asn Val Ile Phe Gln Asn Ala Tyr Val Lys Phe Leu Leu Ala Arg Ile
500 505 510
Ser Asp Asp Ser Cys Cys Ser Asn Cys Ser Gly Asp Cys Leu Ser Gln
515 520 525
Asp Ile Pro Cys Ala Cys Ala Gly Glu Thr Gly Gly Glu Phe Ala Tyr
530 535 540
Thr Ser Gly Gly Leu Leu Lys Glu Lys Phe Leu Glu Ser Cys Ile Ser
545 550 555 560
Met Ser Cys Glu Pro Gln Lys His Gly Leu Val Tyr Cys Gln Asp Cys
565 570 575
Pro Leu Glu Arg Ser Lys Asn Asn Ser Val Ser Gly Leu Cys Lys Gly
580 585 590
His Leu Val Arg Lys Phe Ile Lys Glu Cys Trp His Lys Cys Gly Cys
595 600 605
Ser Arg Gly Cys Gly Asn Arg Val Ile Gln Arg Gly Ile Ala Val Pro
610 615 620
Leu Gln Val Phe Met Thr Ala Asp Gly Lys Gly Trp Gly Leu Arg Ala
625 630 635 640
Leu Glu Asp Leu Pro Arg Gly Ala Phe Val Cys Glu Tyr Val Gly Glu
645 650 655
Ile Val Thr Asn Thr Glu Leu Tyr Glu Arg Asn Thr Gln Thr Ala Ser
660 665 670
Glu Arg His Thr Tyr Pro Val Leu Leu Asp Ala Asp Trp Gly Ser Glu
675 680 685
Gly Val Leu Lys Asp Glu Glu Ala Leu Cys Leu Asp Ala Thr Tyr Tyr
690 695 700
Gly Asn Ile Ala Arg Phe Ile Asn His Arg Cys Tyr Glu Gly Asn Leu
705 710 715 720
Ile Glu Ile Pro Val Glu Val Glu Thr Pro Asp His His Tyr Tyr His
725 730 735
Ile Ala Phe Phe Thr Thr Arg Lys Val Asn Ala Leu Glu Glu Leu Thr
740 745 750
Trp Asp Tyr Gly Ile Asp Phe Thr Asp His Thr His Pro Val Lys Ala
755 760 765
Phe Lys Cys Cys Cys Gly Ser Lys Ser Cys Arg Asp Thr Gly Leu
770 775 780
<210> 68
<211> 865
<212> PRT
<213> 马铃薯
<400> 68
Met Pro Val Asn Pro Arg Val Lys Lys Ala Phe Arg Ala Met Lys Ser
1 5 10 15
Ile Gly Ile Ser Glu Glu Lys Val Lys Pro Ile Leu Lys Ser Leu Leu
20 25 30
Lys Leu Tyr Asp Lys Asn Trp Glu Leu Ile Glu Glu Glu Asn Tyr Arg
35 40 45
Ala Leu Ala Asp Ala Ile Phe Glu Lys Glu Asp Ser Glu Val Ala Glu
50 55 60
His Lys Lys Pro Glu Asn Asn Glu Val Arg Asp Met Pro Leu Val Gln
65 70 75 80
Arg Glu Glu Val Leu Glu Glu Glu Ala Val Pro Glu Glu Pro Glu Arg
85 90 95
Pro Leu Lys Arg Leu Arg Leu Arg Tyr Gln Glu Gly Gln Ala Ser Pro
100 105 110
Ser Ser Asn Asn Ser Ser Ala Gly Thr Ser Leu Lys Arg Pro Arg Arg
115 120 125
Glu Glu Glu Gly Glu Leu Ser Gly Pro Arg Tyr Gln Asn Gln Ser Gln
130 135 140
Gly Glu Ala Asn Pro Ser Ser Val Arg Lys Asn Leu Arg Leu Asn Glu
145 150 155 160
Thr Gln Thr Ser Pro Ile Ala Ser Arg Gly Gln Asn Ser Val Ala Ala
165 170 175
Lys Ser Ser His Ala Val Lys Leu Lys Glu Pro Lys Thr Glu Pro Gly
180 185 190
Val Glu Leu Ser Ser Lys Gln Lys Met Ser Gly Ser Leu Ala Leu Ile
195 200 205
Lys Pro Lys Asp Glu Pro Tyr Thr Asp Asp Met Pro Gln Phe Glu Val
210 215 220
Pro Ile Ala Val Ile His Pro Glu Leu Ser Asn Lys Gly Asp Thr Ser
225 230 235 240
Ser Gly Asn Thr Ser Arg Arg His Ser Glu Thr Pro Glu Leu Leu Ala
245 250 255
Ile Glu Leu Arg Gly Gly Arg Asp Ser Ser Glu Glu Ile Thr Thr Ser
260 265 270
Leu Asn Gly Val Lys Thr Ser Arg Glu Leu Ile Glu Val Gln Asp Arg
275 280 285
Cys His Thr Asp Val Asp Ile Ala Ser Ser Pro Ser Gly Glu Val Lys
290 295 300
Ile Ser Ile Asn Trp Asp Pro Ala Leu Cys Arg Ser Ser Asp Phe His
305 310 315 320
Thr Pro Ser Val Glu Ser Val Met Lys Met Val Glu Leu Lys Cys Leu
325 330 335
Lys Ser Tyr Lys Ile Leu Asp Pro Asn Phe Ser Leu Met Lys Leu Met
340 345 350
Lys Asp Met Cys Glu Cys Val Leu Glu Leu Gly Thr Gln His Ser Pro
355 360 365
Glu Leu Gln Ser Thr Thr Asp Val Ala Ala Glu Asn Asp Phe Gly Ser
370 375 380
Arg Ser Met Thr Val Asn Ser Ser Asn Gly Phe Met Asn Phe Glu Ile
385 390 395 400
Asp Ala Gly Asp Ala Gln Pro Lys Ile Pro Arg Pro Ser Pro Pro Arg
405 410 415
Ile Gly Glu Asp Tyr Ile Gln Ala Gly Gln Ile Ala Ser Met Gly Asn
420 425 430
Cys Gly Ser Thr Pro Glu Thr Asp Gln Asn Gly Ile Glu Gln Thr Asn
435 440 445
Pro Trp Ser Met Glu Ala Pro Cys Glu Leu Ile Leu Gly Glu Ile Gly
450 455 460
Thr Phe Asp Ser Leu Asn Glu Leu Leu Asn Ser Asp Leu Gly Ala Gly
465 470 475 480
Glu Ala Gln Pro Glu Ile Pro His Leu Asn Ser Tyr Phe Gly Glu Asp
485 490 495
Ser Thr Gln Ala Asp His Thr Ala Ser Met Gly Asn Cys Gly Ile Ala
500 505 510
Pro Glu Thr Ser Gln Ser Arg Leu Glu Glu Val Val Ser His Glu Ala
515 520 525
Thr Pro Arg Asp Val Gly Ser Val Glu Val Ile Asp Ile Thr Lys Gly
530 535 540
Gln Glu Asn Val Ile Ile Ser Leu Val Asn Glu Val Asn Ser Asn His
545 550 555 560
Pro Pro Ser Phe His Tyr Ile Ala Ser Asn Val Val Phe Gln Asn Ala
565 570 575
Tyr Val Asn Phe Ser Leu Ala Arg Ile Gly Asp Asp Asn Ser Cys Ser
580 585 590
Thr Cys Ser Gly Asp Cys Leu Ser Leu Ser Thr Pro Cys Ala Cys Ala
595 600 605
His Ile Thr Gly Gly Asp Phe Ala Tyr Thr Lys Glu Gly Leu Ile Lys
610 615 620
Glu Glu Phe Leu Lys Glu Cys Ile Ser Met Asn Arg Asp Pro Lys Lys
625 630 635 640
His Cys Gln Phe Phe Cys Lys Glu Cys Pro Leu Glu Arg Ser Lys Asn
645 650 655
Glu Asp Ile Ile Glu Ala Cys Lys Gly His Leu Met Arg Asn Phe Ile
660 665 670
Lys Glu Cys Trp Trp Lys Cys Gly Cys Ser Lys Gln Cys Gly Asn Arg
675 680 685
Val Val Gln Arg Gly Ile Ser His Lys Leu Gln Val Phe Met Thr Pro
690 695 700
Glu Gly Lys Gly Trp Gly Leu Arg Thr Leu Glu Asp Leu Pro Arg Gly
705 710 715 720
Ala Phe Val Cys Glu Tyr Val Gly Glu Val Leu Thr Asn Thr Glu Leu
725 730 735
Phe Asp Arg Val Ser Arg Ser Pro Asn Gly Glu Glu His Ser Tyr Pro
740 745 750
Ala Leu Leu Asp Ala Asp Trp Gly Ser Glu Gly Val Leu Lys Asp Glu
755 760 765
Glu Ala Leu Cys Leu Asp Ala Thr Phe Tyr Gly Asn Val Ala Arg Phe
770 775 780
Ile Asn His Arg Cys Phe Asp Ser Asn Leu Val Glu Ile Pro Val Glu
785 790 795 800
Ile Glu Thr Pro Asp His His Tyr Tyr His Leu Ala Phe Phe Thr Thr
805 810 815
Arg Lys Ile Lys Ala Met Glu Glu Leu Thr Trp Asp Tyr Gly Ile Asp
820 825 830
Phe Asp Asp Leu Glu His Pro Val Lys Ala Phe Ser Cys His Cys Ser
835 840 845
Ser Lys Phe Cys Arg Asn Met Lys Arg Pro Ser Arg Ser Arg Ser Arg
850 855 860
Arg
865
<210> 69
<211> 824
<212> PRT
<213> 菜豆
<400> 69
Met Ala Pro Asn Lys Arg Val Val Ala Ala Tyr Arg Ala Met Gly Ser
1 5 10 15
Leu Gly Ile Asp Glu Ser Lys Val Lys Ser Ala Leu Lys Lys Leu Leu
20 25 30
Lys Val Phe Asp Lys Asn Trp Glu Leu Ile Glu Ala Glu Asn Tyr Arg
35 40 45
Val Leu Ala Asp Ala Ile Phe Glu Glu Asp Asp Asn Thr Val Pro Lys
50 55 60
Leu Lys Lys Lys Ile Gln Ser Asp Asp Val His Ser Thr Cys His Ser
65 70 75 80
Leu Pro Ser Phe Ser Asn Asn Gln Glu Asp Glu Thr Glu Ser Glu Glu
85 90 95
Ala Gln Met His Asn Glu Arg Ala Gln Pro Leu Lys Arg Leu Arg Leu
100 105 110
Arg Asp Gln Glu Ser Gln Pro Leu His Pro Leu Thr Asn Ser Ala Thr
115 120 125
Ser Pro Pro Ser Lys Arg Pro Lys Leu Glu Asp Asn Ala Leu His Gln
130 135 140
Gly Ser Phe Gly Lys Lys Pro Gln Asn Lys Pro Glu Ser Ser Asp Gly
145 150 155 160
Asn Pro Arg Ile Glu Ala Pro Ser Leu Arg Leu Pro Asp Asp Ile Val
165 170 175
Asp Lys Gly Lys Gln Pro Ala Ser Thr Gln Val Leu Gln Arg Gly Arg
180 185 190
Lys Leu Thr Ser Gly Arg Ser Ser Pro Ser Thr Pro Ser Lys Glu Pro
195 200 205
Thr Val Glu Ser Gly Lys Phe Leu Leu Pro Asn Asn Met Met Pro Arg
210 215 220
Thr Gln Ala Leu Ile Ile Pro Lys Asp Glu Pro Ile Asp Glu Val Pro
225 230 235 240
Asp Tyr Glu Met Pro Ile Ala Val Ile Pro Pro Glu Pro Ser Ser Val
245 250 255
Arg Asp Ser Ser Leu Lys Asn Gly Val Ala Gly Lys His Val Ser His
260 265 270
Val Thr Val Thr Ser Ser Glu Cys Arg Asp Gly Val Arg Asp Glu Asp
275 280 285
Ala Ser Pro Thr Ser Asn Glu Glu Ala Thr Cys Asn Val Glu Ile Ala
290 295 300
Ser Ser Thr Leu Gly Glu Val Lys Leu Ser Leu Ser Cys Ser Ser Ala
305 310 315 320
Leu Trp Gly Ser Lys Phe His Met Pro Arg Arg Asp Glu Ile Ile Glu
325 330 335
Met Met Glu Glu Lys Cys Leu Phe Ser Tyr Lys Ile Ala Asp Pro Asn
340 345 350
Phe Ser Val Met Lys Leu Leu Arg Asp Leu Cys Asp Cys Met Leu Glu
355 360 365
Phe Gly Asn Asn Ser Asn Asn Asp Pro Glu Glu Ser Ser Ile Leu Arg
370 375 380
Ser Asn Val Asp Val Ser Lys Glu Ser Gln Glu Pro Gly Thr Leu Asp
385 390 395 400
Val Val Arg Asn Lys Asp Leu Asp Met Leu Ser Asp Val Ser Asn Gly
405 410 415
Pro Ile Asn Val Ser Pro Ser Ala Ala Val Ala Ser Pro Gln Ser Phe
420 425 430
Leu Leu Leu Ala Asp Leu Asn Gly Val Asn Asp Ala Val Leu Asp Ser
435 440 445
Lys Met Asp Gln Thr Thr Asn Asp Phe Ser Gln Cys Asn Val Arg Lys
450 455 460
Glu Leu Glu Cys Pro Ile Ser Pro Asn Ser His Ser Leu Val Ile Val
465 470 475 480
Pro Gln Asp Gln Leu Thr Ala Asp Asp Ile Arg Ser Phe Tyr Asp Ile
485 490 495
Ser Asp Leu Thr Lys Gly Glu Glu Asn Val Glu Ile Pro Trp Val Asn
500 505 510
Glu Tyr Thr Asn Asp Phe Pro Pro Ser Phe Asn Tyr Ile Pro Gln Asn
515 520 525
Leu Val Phe Gln Asp Ala Tyr Val Asn Ile Ser Leu Ser Arg Val Gly
530 535 540
Ser Glu Asp Cys Cys Ser Thr Cys Ala Gly Asn Cys Val Leu Ser Ile
545 550 555 560
Pro Cys Ala Cys Ala Asn Lys Thr Gly Gly Glu Phe Ala Tyr Gly Ser
565 570 575
Arg Gly Leu Leu Lys Glu Gln Phe Leu Asn Ser Cys Ile Ala Ile Ser
580 585 590
Arg Asn Pro Gln Lys His Phe Phe Tyr Cys Lys Asn Cys Pro Leu Asp
595 600 605
Arg Ile Lys Asn Asp Gly Gly Leu Glu Pro Cys Lys Gly His Leu Lys
610 615 620
Arg Lys Phe Ile Lys Glu Cys Trp Ser Lys Cys Gly Cys Gly Lys His
625 630 635 640
Cys Gly Asn Arg Val Ile Gln Arg Gly Met Thr Cys Lys Leu Gln Val
645 650 655
Phe Phe Thr Ser Glu Gly Lys Gly Trp Gly Leu Arg Thr Leu Glu Asp
660 665 670
Leu Pro Lys Gly Ala Phe Val Cys Glu Phe Val Gly Glu Ile Leu Ser
675 680 685
Ile Lys Glu Leu Tyr Glu Arg Asn Met Lys Cys Thr Gly Glu Gly Lys
690 695 700
His Thr Tyr Pro Val Leu Leu Asp Ala Asn Trp Asp Ser Gly His Val
705 710 715 720
Asn Asn Lys Glu Ala Leu Cys Leu Asp Ala Ala Ser Phe Gly Asn Ile
725 730 735
Ala Arg Phe Ile Asn His Arg Cys Phe Asp Ala Asn Leu Val Glu Ile
740 745 750
Pro Val Glu Val Glu Asp Pro Gly His Tyr Tyr Tyr His Phe Ala Phe
755 760 765
Phe Thr Ser Arg Lys Ile Ser Ala His Glu Glu Leu Thr Trp Asp Tyr
770 775 780
Gly Ile Asp Phe Asp Asp His Asp His Pro Leu Lys Leu Phe Gln Cys
785 790 795 800
Ser Cys Gly Ser Lys Phe Cys Arg His Met Lys Arg Ser Asn Arg Ser
805 810 815
Asn Ile Ser Ala Ser Ile Ser Glu
820
<210> 70
<211> 848
<212> PRT
<213> 葡萄
<400> 70
Met Ala Arg Asn Pro Arg Val Leu Ala Ala Tyr Arg Ala Met Arg Ala
1 5 10 15
Leu Gly Ile Ala Glu Ala Thr Val Lys Pro Val Leu Lys Asn Leu Leu
20 25 30
Arg Leu Tyr Glu Lys Asn Trp Glu Leu Ile Glu Glu Glu Asn Tyr Arg
35 40 45
Ala Leu Ala Asp Ala Ile Phe Glu Tyr Glu Glu Thr Lys Gln Asp Asn
50 55 60
Ile Leu Gly Gly Glu Thr Gln Leu His Asp Glu Pro Ala Arg Pro Leu
65 70 75 80
Lys Arg Leu Arg Leu Arg Asn Gln Glu Ser Gln Val Ser Pro Ser Leu
85 90 95
Ala Asn Ser Ser Gln Thr Leu Gly Gly Ala Val Met Lys Arg Pro Lys
100 105 110
Leu Glu Asp Ala Glu Gln Pro Gln Thr Leu Ala Glu Arg Gln Pro Gln
115 120 125
Gly Ile Ala Glu Thr Pro Glu Pro Ser Val Gly Asn Ile Arg Pro Glu
130 135 140
Leu His Pro Val Ser Ser Pro Gln Ala His Leu Val Asn Lys Gly Lys
145 150 155 160
Gln Pro Ala Leu Pro Gln Pro Leu Ala Val Gln Gly Arg Ser Asp Leu
165 170 175
Ser Pro Thr Ser Ala Thr Lys Arg Ala Glu Ser Asp Leu Leu His Thr
180 185 190
Gln Gln Arg Leu Arg Asp Lys Gly Lys Glu Pro Leu Ser Pro Gln Ile
195 200 205
Ala Ala Lys Glu Lys Arg Ser Ile Pro Val Arg Ser Phe His Leu Asn
210 215 220
Ala Glu Pro Gly Ile Ile Leu Ser Pro Lys Gln Lys Val His Asp Thr
225 230 235 240
Pro Ala Leu Met Lys Pro Lys Asp Glu Pro Phe Thr Asp Asp Ile Leu
245 250 255
Gln Leu Glu Val Pro Ile Ala Val Ile His Pro Asp Pro Leu His Lys
260 265 270
Gly Asn Leu Pro Glu Asn Tyr Ser Thr Gly Lys Leu Asp Gly Pro Gln
275 280 285
Pro Pro Val Asn Ser Arg Val Asp Gly Glu Asp Glu Val Asn Gly Gly
290 295 300
Pro Ala Ser Ser Ser Gly Ala Gly Thr Asn Cys Glu Leu Ala Asn Ile
305 310 315 320
Ser Asn Leu Glu Ile Ala Ser Ser Pro Leu Gly Glu Val Lys Ile Ser
325 330 335
Leu Ser Cys Asn Ser Ala Leu Gly Lys Pro Asp Phe Arg Met Pro Ser
340 345 350
Leu Asp Thr Leu Leu Lys Leu Val Glu Asp Lys Cys Leu Arg Ser Tyr
355 360 365
Lys Ile Ile Asp Pro Asn Phe Ser Val Thr Lys Leu Met Arg Asp Met
370 375 380
Cys Asp Cys Phe Leu Glu Leu Gly Thr His Thr Glu Glu Ser His Glu
385 390 395 400
Gly Ser Ile Asn Thr Thr Pro Thr Gly Asp Leu Leu Gly Lys Ser Thr
405 410 415
Ala Pro Asp Ala Val Gly Ser Cys Gly Asp Glu Glu Asn Phe Ser Met
420 425 430
Ser Ser Cys Ile Thr Asn Gly Ser Phe Lys Ile Gln Cys Ser Thr Glu
435 440 445
Val Ala Val Pro Gln Ile Pro Arg Leu Leu Ser Ser Ser Leu Asn Gly
450 455 460
Leu Gly Asp His Ile Gln Leu Asp Ser Lys Ile Thr Glu Asn Ser Cys
465 470 475 480
Arg Glu Asn Gly Gln Glu Lys Glu Thr Asn Gly Pro Asn Asn Ala Asn
485 490 495
Ser Leu Ser Leu Val Val Val Gln Gln Arg Gln Leu Thr Pro Asp Asp
500 505 510
Ile Arg Phe Ile His Asp Val Asp Asp Ile Thr Lys Gly Glu Glu Lys
515 520 525
Val Arg Ile Pro Leu Val Asn Glu Thr Asn Ser Glu Phe Pro Thr Pro
530 535 540
Phe His Tyr Ile Ser Gln Asn Leu Val Phe Gln Asn Ala Tyr Met Asn
545 550 555 560
Leu Ser Leu Ala Arg Ile Gly Ile Glu Asn Cys Cys Ser Thr Cys Phe
565 570 575
Gly Asp Cys Leu Ser Ser Ser Thr Pro Cys Ala Cys Ala Cys Glu Ser
580 585 590
Gly Gly Asp Phe Ala Tyr Thr Leu Glu Gly Leu Val Lys Glu Asp Phe
595 600 605
Leu Glu Glu Cys Ile Ser Arg Asn Arg Asp Pro Gln Gln His Gln Leu
610 615 620
Ala Phe Cys Gln Glu Cys Pro Leu Glu Arg Ser Lys Ala Glu Asp Ile
625 630 635 640
Leu Glu Pro Cys Lys Gly His Ile Val Arg Lys Phe Ile Lys Glu Cys
645 650 655
Trp Ser Lys Cys Gly Cys Ser Lys Gln Cys Arg Asn Arg Leu Val Gln
660 665 670
Arg Gly Ile Thr Cys Asn Phe Gln Val Phe Leu Thr Pro Asp Gly Lys
675 680 685
Gly Trp Gly Leu Arg Thr Leu Glu Asp Leu Pro Lys Gly Ser Phe Val
690 695 700
Cys Glu Tyr Val Gly Glu Ile Leu Thr Thr Val Glu Leu Tyr Glu Arg
705 710 715 720
Asn Met Gln Ser Thr Ser Arg Gly Lys Gln Thr Tyr Pro Val Leu Leu
725 730 735
Asp Ala Asp Trp Ala Leu Arg Gly Ile Leu Lys Asp Glu Glu Ala Leu
740 745 750
Cys Leu Asp Ala Thr Phe Tyr Gly Asn Val Ala Arg Phe Ile Asn His
755 760 765
Arg Cys Leu Asp Ala Asn Leu Val Glu Ile Pro Val Glu Val Glu Ser
770 775 780
Pro Asp His His Tyr Tyr His Leu Ala Leu Phe Thr Thr Arg Lys Val
785 790 795 800
Asn Ala Leu Glu Glu Leu Thr Trp Asp Tyr Gly Ile Asp Phe Asp Asp
805 810 815
Gln Asp His Pro Val Lys Thr Phe Arg Cys Cys Cys Gly Ser Lys Phe
820 825 830
Cys Arg Asn Met Lys Arg Thr Arg Ser Arg Ser Ala Leu Ser Leu Gly
835 840 845
<210> 71
<211> 811
<212> PRT
<213> 可可
<400> 71
Met Ala Pro Asn Pro Arg Val Val Gln Ala Phe Arg Ser Met Lys Glu
1 5 10 15
Ile Gly Ile Thr Glu Glu Lys Val Lys Pro Val Leu Lys Lys Leu Leu
20 25 30
Lys Val Tyr Asp Lys Asn Trp Glu Leu Ile Ala Ala Glu Asn Tyr Arg
35 40 45
Val Leu Ala Asp Ala Ile Phe Glu Glu Glu Asp Asn Lys Val Ser Glu
50 55 60
Pro Lys Lys Gly Gln Lys Cys Asp Glu Glu Glu Asp Thr Met Glu Glu
65 70 75 80
Gly Ala Val Pro Asp Glu Leu Val Arg Pro Leu Lys Arg Ile Arg Leu
85 90 95
Lys Asn Gln Glu Gly Leu Ala Ser Ser Ser His Asn Asn Gly Ser Thr
100 105 110
Asp Val Ala Gly Pro Phe Leu Lys Lys Pro Lys Val Glu Glu Asp Glu
115 120 125
Leu Pro Pro Ala Ser Leu Arg Gln Gln Ser Leu Gln Cys Asn Val Gly
130 135 140
Asn Met Thr Glu Cys Leu Pro Ala Ser Pro Gly Cys Val Ser Leu Gln
145 150 155 160
Pro Thr Ala Pro Gly Pro Val Ser Pro His Gln Gly Gly Arg Asn Lys
165 170 175
Gly Lys Gln Pro Val Ala Pro Met Pro Leu Ala Val Leu Glu Gly Tyr
180 185 190
Asp Gln Asn Leu His Ser Thr Gln Met His Val Ser Tyr Lys Gly Lys
195 200 205
Glu Pro Met Ser Pro His Val Thr Tyr Asn Glu Lys Gly Pro Glu Arg
210 215 220
Val Ser Leu Ala Leu Cys Ile Lys Asp Pro Ala Pro Cys Pro Gly Ile
225 230 235 240
Ile Thr Lys Lys Arg Met Pro Asp Thr His Ala Leu Ile Ile Pro Lys
245 250 255
Glu Glu Pro Phe Thr Asp Asp Met Pro Gln Asp Glu Val Pro Ile Ala
260 265 270
Val Ile His Pro Asp Ser Leu Ser Arg Arg Asp Ser Pro Ile Gly His
275 280 285
Val Ser Thr Gly Lys Ser Asn Trp Gln Glu His Pro Glu Ser Leu Phe
290 295 300
Ala Asp Glu Asn Val Gly Ala Gly Ala Ser Ala Ser Met Ser Glu Arg
305 310 315 320
His Ile Ser Cys Glu Leu Ala Thr Val Pro Asp Glu Ile Pro Ser Ser
325 330 335
Leu Glu Ile Ala Ser Ser Pro Leu Gly Glu Val Lys Ile Ser Leu Ser
340 345 350
Tyr Asn Ser Ala Leu Gly Arg Ser Asn Phe Gln Leu Pro Ser Ile Asp
355 360 365
Glu Leu Arg Glu Leu Met Glu Gln Arg Cys Leu Arg Ser Tyr Lys Leu
370 375 380
Ile Asp Pro Thr Phe Asp Val Ile Lys Ile Leu Asn Asp Met Cys Glu
385 390 395 400
Cys Ile Ser Glu Leu Ala Thr Asn Ser Ser Asn Gln Ser Gln Glu Gly
405 410 415
Asn Val Met Pro Ala Leu Asp Leu Leu Lys Lys Ser Pro Ala Arg Asp
420 425 430
Ala Leu Asp Ala Glu Ser Asn Lys Glu Asn Gly Cys Leu Pro Ala Lys
435 440 445
Met Leu Asn Gly Ala Leu Asp Val Gln Cys Ser Ser Asn Gly Cys Val
450 455 460
Asp Asn Val Glu Gly Lys Glu Leu Val Val Val Gln Gln His Gln Leu
465 470 475 480
Thr Ser Asn Glu Leu Arg Trp Leu His Asp Ala Ser Asp Ile Thr Lys
485 490 495
Gly Glu Glu Lys Val Glu Ile Ser Trp Val Asn Glu Ile Asn Lys Asp
500 505 510
Phe Pro Pro Pro Phe His Tyr Ile Ser Glu Asn Leu Val Phe Gln Asn
515 520 525
Ala Tyr Val Lys Phe Ser Leu Ser Arg Ile Gly Asp Glu Ser Cys Cys
530 535 540
Pro Thr Cys Phe Gly Asp Cys Leu Leu Ser Glu Gln Pro Cys Ala Cys
545 550 555 560
Ala Cys Gln Ala Gly Gly Lys Phe Val Tyr Thr Ser Ala Gly Val Val
565 570 575
Arg Glu Asp Phe Leu Glu Glu Cys Ile Ser Met Thr Arg Asp Pro Gln
580 585 590
Arg Gln Cys Phe Leu Asn Cys Thr Glu Cys Pro Leu Glu Arg Ser Lys
595 600 605
Lys Asp Asp Phe Pro Glu Pro Cys Lys Gly His Leu Lys Arg Lys Val
610 615 620
Ile Lys Glu Cys Trp Ser Lys Cys Gly Cys Asn Lys Gln Cys Gly Asn
625 630 635 640
Arg Val Val His Arg Gly Val Asn Tyr Lys Leu Gln Val Phe Leu Thr
645 650 655
Pro Asp Gly Lys Gly Trp Gly Leu Arg Thr Leu Glu Lys Leu Pro Lys
660 665 670
Gly Ala Phe Ile Cys Glu Phe Val Gly Glu Ile Leu Thr Ile Ser Glu
675 680 685
Leu Tyr Ala Arg Asn Thr Glu Lys His Thr Cys Pro Ile Leu Leu Asp
690 695 700
Ala Tyr Trp Gly Leu Lys Gly Val Ser Lys Asp Glu Glu Ala Leu Cys
705 710 715 720
Leu Asp Ala Thr Gly Tyr Gly Asn Val Ala Arg Phe Ile Asn His Arg
725 730 735
Cys Leu Asp Ala Asn Leu Ile Glu Ile Pro Val Glu Val Glu Thr Pro
740 745 750
Asp Leu His Tyr Tyr His Leu Ala Phe Phe Thr Thr Arg Glu Ile Asp
755 760 765
Ala Leu Glu Glu Leu Thr Trp Val Ser Glu Trp Asp Tyr Gly Ile Asp
770 775 780
Phe Asp Asp Leu Asp His Pro Val Lys Ala Phe Arg Cys Arg Cys Gly
785 790 795 800
Ser Lys Phe Cys Arg Asn Met Lys Arg Ser Thr
805 810
<210> 72
<211> 761
<212> PRT
<213> 乌拉尔图小麦
<400> 72
Met Gly Ser Ser Arg Gly Asn Arg Asn Val Arg Ala Arg Arg Ala Val
1 5 10 15
Lys Ala Met Lys Leu Leu Gly Ile Ser Arg Glu Gln Thr Ala Pro Val
20 25 30
Leu Arg Arg Leu Val Glu Leu Tyr Asp Asp Asn Trp Gln Leu Ile Glu
35 40 45
Ala Glu Ser Tyr Arg Ala Leu Ala Asp Ala Ile Phe Asp Glu Gln Ala
50 55 60
Asn Gly Gly Pro Ala Asn Gly Gly Asp Gln Val Gln Gly Gln Glu Glu
65 70 75 80
Leu Asp Leu Glu Leu Glu Glu Thr Gly Thr Met Pro Glu Met Phe Thr
85 90 95
Pro Glu Asp Asp Asp Gln Arg Pro Ser Thr Ser Leu Ala Val Val Arg
100 105 110
Lys Asp Pro Asp Asn Arg Ile Thr Ala Pro Arg Ser Arg Thr Gly Ser
115 120 125
Arg Ala Asn Leu Leu Gly Ser Asp Pro Thr Gln Ala Ala Ser Pro Thr
130 135 140
Gln Thr Arg Ala Leu Ser Lys Gln Arg Gln Met Gln Met Gln Met Met
145 150 155 160
Asp Glu Asp Phe Gln Gln Pro Val Phe Leu Arg Glu Pro Lys Pro Glu
165 170 175
Pro Val Asp Met Asp Ala Val Asp Cys Leu Asn Val Gln Pro Gly Leu
180 185 190
Ile His Arg Ser Arg Lys Arg Ser Ala Pro Ser Ser Glu Phe Leu Ala
195 200 205
Leu Pro Pro Pro Glu Gln Thr Pro Lys Gln Ile Ser Glu Gly Asp Lys
210 215 220
Asn Arg Thr Thr Gln His Cys Arg Asn Arg Glu Met Pro Thr Leu Ser
225 230 235 240
Val Glu Pro Ala Asn Ser Thr Met Asn Asn Gly Thr Gly Ser Gly Asp
245 250 255
Gly Asn Val Gln Glu Ala Pro Cys Leu Asp Ile Asp Ile Ala Ser Ser
260 265 270
Pro Arg Gly Asp Val Lys Met Ser Leu Lys Phe Cys Ile Asp Pro Ser
275 280 285
Lys Phe His Met Pro Ala Leu Glu Ala Val Leu Lys Met Val Glu Asp
290 295 300
Lys Cys Leu Arg Ser His Lys Val Leu Pro Pro Asp Phe Ser Ile Arg
305 310 315 320
Gly Leu Met Thr Glu Met Cys Gln Cys Val Val Gln Leu Gly Thr Lys
325 330 335
His Thr Ala Glu His Asn Thr Gln Ser Asp Thr Val Gly Asn Gly Ser
340 345 350
Leu Ser Glu Asn Ala Ile Asn Arg Lys Arg Lys Ala Arg Glu Glu Pro
355 360 365
Leu Val Ser Lys Gly Ser Glu Ser Gly Pro Ala Asn Ser Thr Leu Ala
370 375 380
Gln Gln His His Leu Ala Leu Ser Val Ser Lys Thr Ile His Asp Ile
385 390 395 400
Thr Asp Ile Ser Lys Gly Glu Glu Asn Val Arg Ile Ser Ile Ala Asp
405 410 415
Glu Ser Gly Arg Glu Lys Cys Pro Pro Ser Phe Tyr Tyr Met Pro Thr
420 425 430
Asn Ala Val Ser Gln Asn Ala Leu Ile Ser Met Phe Leu Ala Lys Ile
435 440 445
Gly Ser Glu Asp Cys Cys Pro Glu Cys Phe Gly Asn Cys Leu Ser Ala
450 455 460
Pro Ala Pro Cys Ala Cys Ala Arg Gln Thr Gly Gly Glu Tyr Ala Tyr
465 470 475 480
Thr Pro Asp Gly Leu Val Lys Pro Ala Phe Ile Asp Glu Cys Val Ser
485 490 495
Met Asn Arg Phe Pro Glu Glu His His Arg Val Tyr Cys Lys Thr Cys
500 505 510
Pro Leu Glu Arg Ser Lys Asn Lys Ala Ser Pro Glu Pro Cys Arg Gly
515 520 525
His Leu Val Arg Lys Phe Ile Lys Glu Cys Trp Ser Lys Cys Gly Cys
530 535 540
Asn Met Gln Cys Gly Asn Arg Val Val Gln Arg Gly Ile Arg Cys Asn
545 550 555 560
Leu Gln Val Phe Phe Thr Glu Glu Gly Arg Gly Trp Gly Leu Arg Thr
565 570 575
Leu Asp Asp Leu Pro Lys Gly Thr Phe Val Cys Glu Tyr Ala Gly Glu
580 585 590
Ile Leu Thr Asn Thr Glu Leu His Glu Arg Ala Ile Gln Asn Met Gln
595 600 605
Asn Ala Arg Tyr Thr His Pro Ile Leu Leu Asp Ala Gly Trp Cys Ser
610 615 620
Gly Gly Glu Pro Lys Asp Glu Glu Ala Leu Cys Leu Asp Gly Thr Phe
625 630 635 640
Tyr Gly Asn Val Gly Arg Phe Ile Asn His Arg Cys Cys Asp Ala Asn
645 650 655
Leu Ala Met Ile Pro Val Glu Val Glu Thr Pro Asp His Gln Tyr Tyr
660 665 670
His Val Ala Phe Phe Thr Ser Lys Lys Val Glu Ala Phe Glu Glu Leu
675 680 685
Thr Trp His Val Cys Asn Leu Cys Ser Tyr Arg Gln Met Ser Gln Arg
690 695 700
Ala Arg Leu Pro Lys Pro Met Tyr Leu Gln Arg Leu Thr Asn Leu Met
705 710 715 720
Asp Tyr Gly Ile Asp Phe Asp Asp Glu Arg Gln Pro Met Lys Ala Phe
725 730 735
Glu Cys Leu Cys Gly Ser Arg Tyr Cys Arg Gly Gly Lys Glu Arg Arg
740 745 750
Arg Ser Arg Val Thr Gly Thr Ala Gly
755 760
<210> 73
<211> 857
<212> PRT
<213> 大豆
<400> 73
Met Ala Pro Thr Pro Ser Gln Lys Glu Arg Ala Leu Ala Ala Cys Arg
1 5 10 15
Ala Met Ser Ser Leu Gly Ile Asp Asp Ser Lys Val Lys Ser Val Leu
20 25 30
Lys Lys Leu Leu Lys Val Tyr Asp Lys Asn Trp Glu Leu Ile Glu Ala
35 40 45
Glu Asn Tyr Arg Val Leu Ala Asp Ala Ile Phe Glu Asp Asp Asp Asn
50 55 60
Met Glu Ala Glu Thr Gly Cys Glu Glu Ala Gln Met His Val Glu Thr
65 70 75 80
Pro Gln Pro Leu Lys Arg Leu Arg Leu Gln Gly Gln Glu Ser Gln Pro
85 90 95
Leu His Pro Pro Pro Asn Gly Ser Pro Ser Pro Ser Ser Lys Lys Leu
100 105 110
Lys Leu Asp Asp Asn Ala Ser Gly Lys Lys Pro Gln Asn Lys Pro Val
115 120 125
Ser Ser Asp Gly Asn Pro Gly Ile Ala Thr Arg Pro Leu Pro Pro Arg
130 135 140
Asp Gly Ile Val Asp Lys Gly Lys Gln Pro Ala Ser Leu Pro Leu Asn
145 150 155 160
His Arg Gly Arg Arg Leu Pro Ser Glu Arg Val Pro Gln Ser Ile Pro
165 170 175
Ser Arg Glu Pro Thr Val Glu Pro Gly Arg Phe Leu Leu Pro Asn Asn
180 185 190
Gln Met Pro Arg Thr Gln Thr Leu Val Ile Pro Lys Asp Glu Pro Ile
195 200 205
Asp Glu Leu Thr Asp Tyr Glu Met Pro Ile Ala Val Ile Pro Pro Glu
210 215 220
Ser Ser Val Arg Asn Ser Ser Ile Lys Asn Gly Val Ala Gly Lys His
225 230 235 240
Ser Gly His Val Thr Val Thr Ser Ser Gln Asn Arg Asp Gly Val Gly
245 250 255
Asp Glu Asp Val Ile Pro Thr Ser Lys Lys Glu Ala Thr Cys Asn Val
260 265 270
Glu Ile Ala Ser Ser Thr Leu Gly Glu Val Lys Leu Ser Leu Ser Tyr
275 280 285
Gly Ser Ala Leu Gln Gly Ser Asp Phe His Ile Pro Ser Arg Asp Gln
290 295 300
Leu Ile Lys Val Met Glu Asp Lys Cys Leu Arg Ser Tyr Lys Ile Thr
305 310 315 320
Asp Pro Asn Phe Ser Val Lys Asn Leu Leu Arg Asp Ile Cys Asp Cys
325 330 335
Met Leu Glu Phe Arg Asn Asp Ser Asn Asp Asp Ser Gln Glu Gly Ser
340 345 350
Val Ile Ser Ser Ser Val Asp Val Ser Lys Glu Pro Arg Ala Pro Gly
355 360 365
Thr Leu Ser Val Val Gly Asn Lys Asp Leu Asp Thr Ser Ser His Phe
370 375 380
Ser Asn Gly Ser Ile Asn Val Lys Ser Ser Asp Asp Leu Val Ser Pro
385 390 395 400
Gly Ser Ile Leu Pro Leu Ala His Pro Asn Gly Leu Ser Asp Ala Val
405 410 415
Pro Val Ser Lys Met Asp Arg Thr Asn Asp Phe Leu Gln Ser Asn Val
420 425 430
Lys Lys Asp Leu Glu Asp Pro Met Ser Pro Asn Ser His Ser Leu Val
435 440 445
Val Val Pro Gln His Gln Leu Thr Ala Asp Asp Ile Arg Ser Phe His
450 455 460
Asp Ala Asn Asp Leu Thr Lys Gly Glu Glu Asn Val Glu Ile Pro Trp
465 470 475 480
Val Asn Glu Thr Thr Asn Asp Phe Ala Pro Ser Phe Asn Tyr Ile Pro
485 490 495
Gln Asn Leu Val Phe Gln Glu Ala Tyr Val Asn Ile Ser Leu Ser Arg
500 505 510
Val Gly Ser Glu Asp Cys Cys Ser Thr Cys Met Gly Asn Cys Val Leu
515 520 525
Ser Ser Ser Cys Ala Cys Ala Asn Lys Thr Gly Gly Glu Phe Ala Tyr
530 535 540
Asn Ala Gln Gly Leu Leu Lys Glu Glu Phe Leu Glu Glu Cys Ile Ala
545 550 555 560
Ile Ser Arg Asn Pro Gln Gln His Leu Phe Tyr Cys Lys Asn Cys Pro
565 570 575
Leu Glu Arg Ser Lys Ser Asp Gly Cys Leu Glu Pro Cys Lys Gly His
580 585 590
Leu Lys Arg Lys Phe Ile Lys Glu Cys Trp Ser Lys Cys Gly Cys Gly
595 600 605
Lys Gln Cys Gly Asn Arg Val Ile Gln Arg Gly Ile Thr Cys His Leu
610 615 620
Gln Val Phe Phe Thr Ser Glu Gly Lys Gly Trp Gly Leu Arg Thr Leu
625 630 635 640
Glu Asp Leu Pro Lys Gly Ala Phe Val Cys Glu Phe Val Gly Glu Ile
645 650 655
Leu Ser Met Lys Glu Leu His Glu Arg Asn Leu Lys Cys Thr Glu Asn
660 665 670
Gly Lys Tyr Thr Cys Pro Val Leu Leu Asp Ala Asn Trp Asp Ser Gly
675 680 685
Tyr Val Lys Asp Glu Glu Ala Leu Cys Leu Asp Ala Ala Ser Phe Gly
690 695 700
Asn Thr Ala Arg Phe Ile Asn His Arg Cys Ser Asp Ala Asn Leu Ile
705 710 715 720
Glu Ile Pro Val Glu Val Glu Asp Pro Gly His Tyr Tyr Tyr His Phe
725 730 735
Ala Phe Phe Thr Ser Arg Lys Ile Ser Ala Gln Glu Glu Leu Thr Trp
740 745 750
Asp Tyr Gly Ile Asp Phe Asp Asp His Asp His Pro Val Lys Leu Phe
755 760 765
Gln Cys Arg Cys Gly Ser Lys Phe Cys Arg Asn Met Lys Arg Tyr Ser
770 775 780
Ser Lys Phe Asn Leu Gln Leu Leu Gly His His His Lys Pro Thr Met
785 790 795 800
Ile Val Met Lys Tyr Arg Ala Pro Leu Gln Tyr Trp Ser Gly Val Leu
805 810 815
Leu Ile Asp Ala Ile Ile Phe His His Ile Ser Phe Ser Ser His Trp
820 825 830
Ile Ala Trp Leu Ile Val Gly Lys Ala Leu Asp Leu Leu Asp Met Arg
835 840 845
Gln Leu Gln Asp Asp Ser Leu Ser Pro
850 855
<210> 74
<211> 741
<212> PRT
<213> 稻
<220>
<223> 粳型
<400> 74
Met Ala Lys Pro Asn Gly Lys Glu Lys Thr Gly Asp Thr Gly Leu Ser
1 5 10 15
Met Ala Pro Pro Lys Ile Ser Lys Asp Arg Phe Asp Ala Ala Ile Arg
20 25 30
Ala Met Ala Asp Ile Gly Ile Leu Lys Glu Thr Ala Ala Pro Val Leu
35 40 45
Asn Asn Leu Leu Asn Leu Phe Asp Tyr Asn Trp Val His Ile Glu Ala
50 55 60
Asp Asn Tyr Leu Ala Leu Ala Asp Ala Ile Phe Cys Asp Ser Asp Pro
65 70 75 80
Lys Glu Gly Gln Lys Arg Gln Ala Asn Glu Thr Asn Leu Asp Ala Asp
85 90 95
Gln Ser Asn Lys Lys Leu Lys Thr Lys Lys Arg Ser Gln Asn Pro Thr
100 105 110
Ser Lys Met His Gly Asn Asp Asn Arg Glu Phe Val Glu Ala Pro Pro
115 120 125
Gln Gln Gly Arg Gly Thr Leu Ser Ala Arg Thr Val Asn Gly Lys Lys
130 135 140
Val Thr Arg Ala His Leu Glu Leu Pro Ser Ser Gln Leu Leu Ile Lys
145 150 155 160
Glu Pro His Thr Cys Pro Ser Ile Ala Lys Asn Thr Thr Ile Val Glu
165 170 175
Asn Asn Ser Ala Val Leu Cys His Gly Gln Asp Leu Gln Thr Phe Glu
180 185 190
Val Pro Val Ala Thr Thr Cys Pro Gln Val Val Ala Pro Ser Thr Arg
195 200 205
Lys Asp Ala Arg Arg Thr Ser Gly Ala Arg His Asp Gln Lys His Glu
210 215 220
Gly Val Ser Gly Ala His Glu Arg Asn Arg Ala Val Ala Cys Ser Asn
225 230 235 240
Gln Glu Ile Val Ser Ser Lys Asp Ser Pro Ser Asn Ile Glu Val Val
245 250 255
Leu Ser Asn Tyr Gly Ala Gly Lys Leu Ser Phe Thr Tyr Asn Ser Ser
260 265 270
Leu Ala Asn Arg Ser Asp Phe His Leu Pro Asp Ile Lys Leu Ile Cys
275 280 285
Lys Lys Met Glu Ala Arg Cys Leu Arg Lys Tyr Lys Ser Leu Glu Pro
290 295 300
Asn Phe Ser Phe Lys Asn Leu Ile Lys Asp Thr Cys Gln Cys Ile Val
305 310 315 320
Glu Ser Ser Gly Pro Arg His Glu Gly Ile Ile Gln Thr Val Pro Ala
325 330 335
Leu Asp Ile Leu Ser Lys Pro Ser Val Pro Gln Ile Leu Gln Ser Asn
340 345 350
Gln Ala Asn Ser Ala Phe Met Pro Pro Asn Asn Val Met Ser Leu Gly
355 360 365
Gly Thr Ser Ser Ser Cys Thr Val Ala Gly Val Ser Gln Asn Ser Ser
370 375 380
Asn Met Pro Val Val Pro His Gln Leu His Ile Gly Ala Asn Arg Pro
385 390 395 400
Pro His Asp Val Asn Asp Ile Thr Lys Gly Glu Glu Arg Leu Arg Ile
405 410 415
Pro Ile Ile Asn Glu Tyr Gly Asn Gly Ile Leu Pro Pro Pro Phe His
420 425 430
Tyr Ile Pro His Asn Ile Thr Leu Gln Glu Ala Tyr Val Asn Ile Ser
435 440 445
Leu Ala Arg Ile Gly Asp Asp Asn Cys Cys Ser Asp Cys Phe Arg Asp
450 455 460
Cys Leu Ala Gln Ser Leu Pro Cys Ala Cys Ala Ala Glu Thr Gly Gly
465 470 475 480
Glu Phe Ala Tyr Thr Thr Asp Gly Leu Leu Lys Gly Ala Phe Leu Asp
485 490 495
Ser Cys Ile Ser Met Ile Arg Glu Pro Leu Lys His Pro His Phe Tyr
500 505 510
Cys Lys Ile Cys Pro Asn Glu Arg Met Lys Ile Glu Val Asn Ser Asp
515 520 525
Ser Ser Asn Thr Glu Met Asn Pro Gly Pro Cys Lys Gly His Leu Thr
530 535 540
Arg Lys Phe Ile Lys Glu Cys Trp Arg Lys Cys Gly Cys Thr Arg Asn
545 550 555 560
Cys Gly Asn Arg Val Val Gln Arg Gly Ile Thr Arg His Leu Gln Val
565 570 575
Phe Leu Thr Pro Glu Lys Lys Gly Trp Gly Leu Arg Ser Thr Glu Lys
580 585 590
Leu Pro Arg Gly Ala Phe Val Cys Glu Tyr Val Gly Glu Ile Leu Thr
595 600 605
Asn Ile Glu Leu Tyr Asp Arg Thr Ile Gln Lys Thr Gly Lys Ala Lys
610 615 620
His Thr Tyr Pro Leu Leu Leu Asp Ala Asp Trp Gly Thr Glu Gly Val
625 630 635 640
Leu Lys Asp Glu Glu Ala Leu Cys Leu Asp Ala Thr Phe Tyr Gly Asn
645 650 655
Val Ala Arg Phe Ile Asn His Arg Cys Phe Asp Ala Asn Ile Ile Gly
660 665 670
Ile Pro Val Glu Ile Glu Thr Pro Asp His His Tyr Tyr His Leu Ala
675 680 685
Phe Phe Thr Thr Arg Ile Ile Glu Pro Phe Glu Glu Leu Thr Trp Asp
690 695 700
Tyr Gly Ile Asp Phe Asp Asp Val Asp His Pro Val Lys Ala Phe Lys
705 710 715 720
Cys His Cys Gly Ser Glu Phe Cys Arg Asp Lys Thr Arg Arg Ser Lys
725 730 735
Ser Arg Ala Arg Val
740
<210> 75
<211> 736
<212> PRT
<213> 稻
<220>
<223> 籼型
<400> 75
Met Gly Ser Lys Ser Asn Thr Glu Arg Ala Arg Lys Ala Leu Glu Ala
1 5 10 15
Met Lys Gln Leu Gly Phe Ser Lys Lys Glu Ala Thr Pro Val Leu Lys
20 25 30
Asn Leu Leu Arg Leu Phe Gly Asn Asn Trp Glu Pro Ile Glu Asp Glu
35 40 45
Cys Tyr Arg Ala Leu Ala Asp Ala Ile Leu Asp Arg His Gln Glu Thr
50 55 60
Ala Ala Asp Arg Gly Cys Ser Ala Thr Arg Pro Thr Pro Asp Asp Asp
65 70 75 80
His His Pro Leu Thr Leu Cys Gly Ala Ser Arg Asp Val Asp Thr Glu
85 90 95
Thr Asp Glu Pro Arg Thr Lys Lys Pro Arg Ala Thr Asn Ser Asp Pro
100 105 110
Gln Ser Pro Pro Ser Leu Thr Asp Asp Gln Asp Val Pro Ala Ala Ile
115 120 125
Ser Pro Pro Ser His Gly Ala Ser Pro Gln Phe Arg Pro Gln Thr Arg
130 135 140
Ala Ser Ala Arg Leu Arg Gln Ala Ser Pro Ser Ser Val Thr Ala Ala
145 150 155 160
His Lys Arg Pro Arg Gln Met Met Asp Glu Asp Phe Gln Asp Ser Ala
165 170 175
Phe Leu Arg Glu Pro Lys Pro Glu Pro Asp Ile Asp Met Asp Ala Ile
180 185 190
Gln Gly Thr Ala Ala Thr Ser Asp Cys Pro Asn Ala His Leu Gly Leu
195 200 205
Ile Asp Tyr Pro Leu Asn Ala Ser Ser Ser Arg Val Ala Leu Pro Leu
210 215 220
Ala Leu Leu Pro Pro Asp Gln Asn Val Pro Gln Ile Ser Gly Pro Lys
225 230 235 240
Lys Arg Ala Ile Gln Pro Cys Ser Lys Val Asn Thr Gly Glu Gly Ser
245 250 255
Ser Val Ile Asp Val Ala Ser Ser Thr Met Gly Glu Val Lys Met Ser
260 265 270
Leu Lys Cys Ser Val Asp Pro Lys Phe Arg Met Pro Ser Leu Glu Ala
275 280 285
Val Phe Lys Met Val Glu Asp Lys Tyr Leu His Ser Tyr Lys Ile Leu
290 295 300
Pro Pro Glu Phe Ser Ile Gly Ser Leu Met Asn Glu Ile Cys Gln Cys
305 310 315 320
Val Val Gln Leu Gly Ser Asp His Ile Ala Glu His Asn Thr Glu Ser
325 330 335
Asp Val Ala Gly Asn Gly Arg Cys Ser Gln Asn Glu Pro Met Thr Gly
340 345 350
Ser Ile Pro Phe Val Lys Pro Ile Ala Cys Glu Asp Gly Gly Asn Arg
355 360 365
Lys Cys Lys Ser Ala Gly Glu Ser Phe Ile Val Glu Asp Ser Glu Asn
370 375 380
Ser Ser Val Ala Lys Gln Lys Thr His Leu Ala Leu Ala Asn Leu Lys
385 390 395 400
Pro Ile His Asp Val Thr Asp Ile Ser Lys Gly Glu Glu Arg Val Arg
405 410 415
Ile Ser Val Ala Asn Glu Phe Ala Ser Glu Lys Cys Pro Pro Ser Phe
420 425 430
Tyr Tyr Ile Arg Gly Asn Leu Val Phe Gln Asn Ala Tyr Val Asn Ile
435 440 445
Ser Val Ala Arg Ile Gly Asp Glu Asp Cys Cys Ala Asp Cys Phe Gly
450 455 460
Asn Cys Leu Ser Ala Pro Ile Pro Cys Ala Cys Thr Arg Glu Thr Gly
465 470 475 480
Gly Glu Tyr Val Tyr Thr Pro Glu Gly Leu Val Arg Thr Pro Phe Leu
485 490 495
Asp Glu Cys Val Ser Met Asn Arg Phe Pro Glu Lys Cys His Lys Phe
500 505 510
Phe Cys Lys Ser Ser Cys Pro Leu Glu Arg Ser Arg Asn Glu Ala Ser
515 520 525
Pro Glu Pro Cys Arg Gly His Leu Ala Arg Lys Phe Ile Lys Glu Cys
530 535 540
Trp Ser Lys Cys Gly Cys Asn Met Gln Cys Gly Asn Arg Val Val Gln
545 550 555 560
Arg Gly Ile Thr Cys Asn Leu Gln Val Phe Phe Thr Gly Glu Gly Lys
565 570 575
Gly Trp Gly Leu Arg Thr Leu Asp Glu Leu Pro Lys Gly Ala Phe Val
580 585 590
Cys Glu Tyr Val Gly Glu Val Leu Thr Ser Thr Glu Leu His Glu Arg
595 600 605
Thr Leu Gln Asn Met Asn Asn Gly Arg His Thr Tyr Pro Val Leu Leu
610 615 620
Asp Ala Asp Trp Gly Ser Glu Gly Val Leu Lys Asp Glu Glu Ala Leu
625 630 635 640
Ser Leu Asp Ser Thr Phe Tyr Gly Asn Val Gly Arg Phe Ile Asn His
645 650 655
Arg Cys Tyr Asp Ala Asn Leu Val Glu Ile Pro Val Glu Val Glu Thr
660 665 670
Pro Asp His His Tyr Tyr His Leu Ala Phe Phe Thr Thr Lys Lys Val
675 680 685
Glu Ala Phe Glu Glu Leu Thr Trp Asp Tyr Gly Ile Asp Phe Gly Asp
690 695 700
Gly Lys Asp Pro Val Lys Ala Phe Gln Cys Leu Cys Gly Ser Arg Tyr
705 710 715 720
Cys Arg Gly Ile Arg His Pro Arg Lys Arg Gly Lys Ala Ala Ala Lys
725 730 735
<210> 76
<211> 720
<212> PRT
<213> 玉蜀黍
<400> 76
Met Gly Ser Asn Thr Glu Arg Ala Arg Lys Ala Leu Gly Ala Met Lys
1 5 10 15
Leu Ile Gly Phe Ser Lys Lys Gln Ala Ile Pro Val Leu Lys Glu Leu
20 25 30
Leu Lys Ile Phe Asn Asn Asn Trp Glu Pro Ile Glu Asp Glu Ser Tyr
35 40 45
Arg Ala Leu Ala Asp Ala Ile Leu Asp Arg Gln Asp Ser Lys Gln Thr
50 55 60
Pro Pro Ser Gln Gln Gly Thr Gln Ala Ala His Ala Asp Leu Glu Pro
65 70 75 80
Leu Arg Ser Thr Arg Asp Asp Arg Gln Gln Tyr Leu Ser Ala Ser Leu
85 90 95
Asp Asp Thr Cys Glu Asp Asp Asn Glu Thr Pro Leu Val Lys Arg Pro
100 105 110
Arg Met Gly Thr Ala Asn Phe Arg Pro Glu Leu Gln Pro Pro Gln Gln
115 120 125
Ser Ser Val Ser Thr Gln Gly Ala Leu Pro Ala Ser Pro Gln Thr Ser
130 135 140
Arg Arg Gln Thr Arg Ser Leu Thr Val Glu Pro His Ala Ala Gly His
145 150 155 160
Glu Tyr Pro Leu Ala Val Asn Asp Ala Leu Val Val Lys Glu Pro Lys
165 170 175
Pro Glu Pro Gln Ile Asp Arg Ala Glu Asp Phe Val Ala Gly Pro Asn
180 185 190
Ala Thr Gln Leu Asn Asp Gly Ser Ser Glu Gln Thr Ser Gly Phe Gly
195 200 205
Ala Arg Gly Asn Ile Val His Gln Ala Gln Ser Leu Gly Arg Ser Leu
210 215 220
Gln Ala Ala Ala Val Cys Lys Asn Gly Val Gly Ser Thr Val Gln Asn
225 230 235 240
Thr His Glu Thr Ser Ile Val Glu Val Asp Val Ala Ser Ser Ala Asn
245 250 255
Gly Glu Val Lys Met Ser Leu Lys Cys Ser Leu Gly Ser Ser Asn Phe
260 265 270
Ser Ile Ser Met Glu Glu Val Phe Lys Met Val Glu Glu Lys Cys Leu
275 280 285
His Ser Tyr Lys Met Leu Pro Ser Asp Leu Ser Ile Gly Lys Leu Leu
290 295 300
Ser Glu Val Cys Gln Ser Val Val Gln Leu Gly Thr Met His Ser Glu
305 310 315 320
Val Asn Arg Asp Ser Gly Ser Leu His Asn Glu Val Val Val Pro Phe
325 330 335
Val Lys Pro Ile Ser Cys Glu Ala Ala Val Gly Ile Ser Asp Asp Val
340 345 350
Ala Gly Gly Ser Ser Val Leu Glu Ser Ser Glu Pro Cys Leu Gln Asn
355 360 365
Ser Leu Val Ala Trp Asn Pro Glu Leu Ala His Cys Asn Gln Lys Thr
370 375 380
Thr His Asp Thr Thr Asp Ile Ser Lys Gly Glu Glu Arg Val Arg Ile
385 390 395 400
Pro Val Val Asn Glu Phe Gly Ser Glu Thr Cys Pro Pro Ser Phe Tyr
405 410 415
Tyr Ile Pro Arg Asn Leu Val Phe Gln Asn Ala Tyr Val Asn Ile Ser
420 425 430
Ile Ser Arg Ile Cys Asp Glu Asp Cys Cys Ala Asp Cys Ser Gly Asn
435 440 445
Cys Leu Ser Ala Pro Val Pro Cys Ser Cys Ala Arg Ile Thr Gly Gly
450 455 460
Glu Phe Ser Tyr Thr Leu Glu Gly Leu Leu Lys Thr Ala Phe Leu Asp
465 470 475 480
Glu Cys Thr Ser Val Asn His Phe Leu Gln Glu His His Arg Phe Tyr
485 490 495
Cys Pro Val Cys Pro Leu Glu Arg Ser Lys Asn Glu Gly Ser Pro Gly
500 505 510
Pro Cys Lys Gly His Ile Val Arg Lys Phe Ile Lys Glu Cys Trp Ser
515 520 525
Lys Cys Gly Cys Gly Met Gln Cys Gly Asn Arg Val Ile Gln Arg Gly
530 535 540
Ile Thr Cys Lys Leu Gln Val Phe Phe Thr Arg Glu Gly Lys Gly Trp
545 550 555 560
Gly Val Arg Thr Val Glu Asp Leu Pro Arg Gly Ala Phe Val Cys Glu
565 570 575
Tyr Val Gly Glu Ile Leu Thr Ser Ala Glu Leu His Glu Arg Ala Ile
580 585 590
Glu Asn Ala Arg Asn Gly Lys His Met His Gln Val Leu Leu Asp Ala
595 600 605
Gly Trp Gly Ser Gly Val Val Leu Arg Asp Glu Asp Ala Cys Ser Leu
610 615 620
Asp Gly Ser Phe Tyr Gly Asn Val Gly Arg Phe Ile Asn His Arg Cys
625 630 635 640
Tyr Asn Ala Asn Leu Val Gln Ile Pro Val Glu Val Glu Thr Pro Asp
645 650 655
His His Tyr His His Leu Ala Phe Phe Thr Asn Lys Lys Val Glu Ala
660 665 670
Phe Glu Glu Leu Thr Trp Asp Tyr Gly Ile Asp Phe Asp Asp Met Glu
675 680 685
Gly Pro Ser Lys Pro Phe Arg Cys Met Cys Gly Ser Arg Tyr Cys Arg
690 695 700
Asp Pro Asn Asn Pro Arg Arg Met Gly Arg Ala Ala Ala Lys Arg Lys
705 710 715 720
<210> 77
<211> 739
<212> PRT
<213> 两色蜀黍
<400> 77
Met Gly Gly Lys Asn Met Glu Arg Ala Arg Lys Ala Leu Asp Ala Met
1 5 10 15
Lys Glu Leu Gly Ile Ser Arg Lys Gln Ala Thr Pro Val Leu Lys Glu
20 25 30
Leu Leu Ala Thr Phe Asp Asn Asn Trp Glu Pro Ile Glu Asp Glu His
35 40 45
Tyr Arg Ala Leu Ala Asp Ala Ile Phe Ala Arg Glu Asp Asn Lys Gln
50 55 60
Thr Ser Pro Ser Gln Gln Gly Ala Gln Ala Ala His Val Asp Ser Glu
65 70 75 80
Pro Asn Gly Ser Thr Trp Asp Asn Arg Gln Gln Tyr Phe Ser Ala Thr
85 90 95
His Asp Asp Thr Gly Glu Asp Asp Asn Glu Thr Pro Leu Val Lys Arg
100 105 110
Pro Arg Met Gly Thr Ala Asn Phe Arg Ser Glu Pro Gln Pro Phe Glu
115 120 125
Pro Gly Pro Gln Gln Ser Thr Val Ser Thr Gln Gly Ala Leu Pro Ala
130 135 140
Ser Pro Gln Ala Ser Arg Arg Gln Ser Arg Ser Leu Ala Val Val Pro
145 150 155 160
His Ala Ala Gly His Glu Tyr Pro Leu Ala Val Asp Asp Ala Leu Ile
165 170 175
Val Lys Glu Pro Lys Pro Glu Pro Gln Ile Asp Ile Ser Glu Val Ser
180 185 190
Val Gly Asp Pro Leu Thr Asp Arg Asp Phe Leu Ala Gly Pro Asp Ala
195 200 205
Ile Arg Leu Asp Asp Gly Ser Ser Gly Ser Gly Ala Arg Gly Cys Ile
210 215 220
Val Asn Gln Pro Gln Ser Leu Gly Arg Ser Leu Gln Pro Ala Pro Val
225 230 235 240
Cys Glu Asn Gly Val Gln Ser Thr Val Gln Asn Thr Gln Glu Thr Ser
245 250 255
Phe Val Glu Val Asp Val Ala Ser Ser Thr Asn Gly Glu Val Lys Met
260 265 270
Ser Leu Lys Cys Ser Leu Asp Ser Ser Asn Phe Ser Ile Ser Met Glu
275 280 285
Glu Val Phe Lys Met Val Glu Glu Lys Cys Leu His Ser Tyr Lys Val
290 295 300
Leu Pro Pro Asp Phe Ser Ile Gly Lys Leu Met Ser Glu Val Cys Gln
305 310 315 320
Ser Val Val Gln Leu Gly Thr Val His Ser Glu Ile Asn Arg Asp Ser
325 330 335
Gly Ser Leu His Asn Glu Ala Val Ala Pro Phe Val Lys Pro Ile Ala
340 345 350
Cys Glu Ala Ser Val Gly Ile Asn Gly Asn Val Ala Gly Gly Ser Ser
355 360 365
Val Leu Glu Thr Ser Glu Pro Cys Leu Gln Asn Ser Leu Val Ala Trp
370 375 380
Asp Pro Glu Leu Ala His Arg Lys Gln Lys Thr Thr His Asp Ile Thr
385 390 395 400
Asp Ile Ser Lys Gly Gly Glu Arg Val Arg Ile Pro Val Val Asn Glu
405 410 415
Phe Gly Ser Glu Thr Cys Pro Pro Ser Phe Tyr Tyr Val Pro Arg Asn
420 425 430
Leu Ile Phe Gln Asn Ala Tyr Val Asn Phe Ser Ile Ala Arg Ile Gly
435 440 445
Asp Glu Asp Cys Cys Ala Asp Cys Ser Gly Asn Cys Leu Ser Ala Ser
450 455 460
Val Pro Cys Ala Cys Ser Arg Leu Thr Gly Gly Glu Phe Pro Tyr Thr
465 470 475 480
Pro Glu Gly Leu Leu Lys Pro Ala Phe Leu Asp Glu Cys Thr Ser Val
485 490 495
Asn His Phe Pro Lys Glu His His Arg Phe Tyr Cys Thr Val Cys Pro
500 505 510
Leu Glu Arg Ser Lys Asn Glu Ala Ser Pro Gly Ala Cys Lys Gly His
515 520 525
Leu Val Arg Lys Phe Ile Lys Glu Cys Trp Ser Lys Cys Gly Cys Gly
530 535 540
Met Gln Cys Gly Asn Arg Val Ile Gln Arg Gly Ile Thr Cys Lys Leu
545 550 555 560
Gln Val Phe Phe Thr Arg Glu Gly Lys Gly Trp Gly Val Arg Thr Val
565 570 575
Glu Asp Leu Pro Lys Gly Ala Phe Val Cys Glu Tyr Val Gly Glu Ile
580 585 590
Leu Thr Ser Ala Glu Leu His Glu Arg Ala Ile Glu Asn Ala Arg Asn
595 600 605
Gly Lys His Met His Gln Val Leu Leu Asp Ala Gly Trp Gly Ser Gly
610 615 620
Val Ser Arg Asp Asp Glu Gly Ser Gly Val Leu Arg Asp Glu Glu Ala
625 630 635 640
Leu Ser Leu Asp Gly Ser Phe Tyr Gly Asn Val Gly Arg Phe Ile Asn
645 650 655
His Arg Cys Tyr Asp Pro Asn Leu Val Gln Ile Pro Val Glu Ile Glu
660 665 670
Thr Pro Asp His His Tyr Tyr His Leu Ala Phe Phe Thr Asn Lys Lys
675 680 685
Val Glu Ala Phe Glu Glu Leu Thr Trp Asp Tyr Gly Ile Asp Phe Asp
690 695 700
Asp Val Glu Gly Pro Ser Lys Pro Phe Arg Cys Met Cys Gly Ser Arg
705 710 715 720
Tyr Cys Arg Asp Pro Asn Asn Thr Arg Arg Met Gly Arg Ala Ala Ser
725 730 735
Lys Arg Lys
<210> 78
<211> 978
<212> PRT
<213> 人工序列
<220>
<223> 合成的构建体
<400> 78
Met Arg Ser Tyr Asn Leu Phe His Glu Ser Asn Pro Thr Ile Ser Phe
1 5 10 15
Ser Phe Val Gly Ala Glu Thr Met Ala Pro Asn Leu His Ile Lys Lys
20 25 30
Ala Phe Met Ala Met Arg Ala Met Gly Ile Glu Asp Ala Arg Val Lys
35 40 45
Pro Val Leu Lys Asn Leu Leu Ala Leu Tyr Glu Lys Asn Trp Glu Leu
50 55 60
Ile Ala Glu Asp Asn Tyr Arg Val Leu Ala Asp Ala Ile Phe Asp Ser
65 70 75 80
His Glu Asp Gln Ala Ile Gln Glu Ser Glu Glu Lys Lys Ala Asp Glu
85 90 95
Val Lys Glu Asp Glu Gly Cys Ala Ala Glu Val Asp Arg Gly Lys Lys
100 105 110
Lys Leu His Glu Ser Ile Glu Asp Asp Glu Asp Val Met Ala Glu Ser
115 120 125
Asp Arg Pro Leu Lys Arg Leu Arg Arg Arg Gly Glu Gly Gly Ser Ala
130 135 140
Leu Ala Ser Pro Ser Leu Gly Ser Pro Thr Leu Glu Gly Pro Ser Ile
145 150 155 160
Asn Asp Glu Glu Asn Ala Pro Ile Leu Leu Pro Tyr His Pro Val Pro
165 170 175
Ile Glu Asn Asp His Asp Ala Gly Glu Leu Ile Leu Thr Lys Val Glu
180 185 190
Pro Ile Thr Asn Met Pro Leu Ser Ser Ile Pro Asp Ser Val Asp Arg
195 200 205
Gly Asp Ser Ser Met Leu Glu Ile Asp Lys Ser Asn Gly His Val Glu
210 215 220
Glu Lys Ala Gly Glu Thr Val Ser Thr Ala Asp Gly Thr Thr Asn Asp
225 230 235 240
Ile Ser Pro Thr Thr Val Ala Arg Phe Ser Asp His Lys Leu Ala Ala
245 250 255
Thr Ile Glu Glu Pro Pro Ala Leu Glu Leu Ala Ser Ser Ala Ser Gly
260 265 270
Glu Val Lys Ile Asn Leu Ser Phe Ala Pro Ala Thr Gly Gly Ser Asn
275 280 285
Pro His Leu Pro Ser Met Glu Glu Leu Arg Arg Ala Met Glu Glu Lys
290 295 300
Cys Leu Arg Ser Tyr Lys Ile Leu Asp Pro Asn Phe Ser Val Leu Gly
305 310 315 320
Phe Met Asn Asp Ile Cys Ser Cys Tyr Leu Asp Leu Ala Thr Asn Gly
325 330 335
Arg Asp Ser Ala Asn Gln Leu Pro Lys Asn Leu Pro Phe Val Thr Thr
340 345 350
Asn Ile Asp Ala Leu Lys Lys Ser Ala Ala Arg Met Ala Tyr Thr Ser
355 360 365
Gln Ala Ser Asn Asp Val Val Glu Ile Cys Ser Asn Glu His Met Arg
370 375 380
Asp Ala Glu Asn Gly Ala Val Gly Asp Ser Met Ala Leu Val Val Val
385 390 395 400
Pro Glu Cys Gln Leu Ser Ala Asp Glu Trp Arg Leu Ile Ser Ser Val
405 410 415
Gly Asp Ile Ser Leu Gly Lys Glu Thr Val Glu Ile Pro Trp Val Asn
420 425 430
Glu Val Asn Asp Lys Val Pro Pro Val Phe His Tyr Ile Ala Gln Ser
435 440 445
Leu Val Tyr Gln Asp Ala Ala Val Lys Phe Ser Leu Gly Asn Ile Arg
450 455 460
Asp Asp Gln Cys Cys Ser Ser Cys Cys Gly Asp Cys Leu Ala Pro Ser
465 470 475 480
Met Ala Cys Arg Cys Ala Thr Ala Phe Asn Gly Phe Ala Tyr Thr Val
485 490 495
Asp Gly Leu Leu Gln Glu Asp Phe Leu Glu Gln Cys Ile Ser Glu Ala
500 505 510
Arg Asp Pro Arg Lys Gln Met Leu Leu Tyr Cys Lys Glu Cys Pro Leu
515 520 525
Glu Lys Ala Lys Lys Glu Val Ile Leu Glu Pro Cys Lys Gly His Leu
530 535 540
Lys Arg Lys Ala Ile Lys Glu Cys Trp Ser Lys Cys Gly Cys Met Lys
545 550 555 560
Asn Cys Gly Asn Arg Val Val Gln Gln Gly Ile His Asn Lys Leu Gln
565 570 575
Val Phe Phe Thr Pro Asn Gly Arg Gly Trp Gly Leu Arg Thr Leu Glu
580 585 590
Lys Leu Pro Lys Gly Ala Phe Val Cys Glu Leu Ala Gly Glu Ile Leu
595 600 605
Thr Ile Pro Glu Leu Phe Gln Arg Ile Ser Asp Arg Pro Thr Ser Pro
610 615 620
Val Ile Leu Asp Ala Tyr Trp Gly Ser Glu Asp Ile Ser Gly Asp Asp
625 630 635 640
Lys Ala Leu Ser Leu Glu Gly Thr His Tyr Gly Asn Ile Ser Arg Phe
645 650 655
Ile Asn His Arg Cys Leu Asp Ala Asn Leu Ile Glu Ile Pro Val His
660 665 670
Ala Glu Thr Thr Asp Ser His Tyr Tyr His Leu Ala Phe Phe Thr Thr
675 680 685
Arg Glu Ile Asp Ala Met Glu Glu Leu Thr Trp Asp Tyr Gly Val Pro
690 695 700
Phe Asn Gln Asp Val Phe Pro Thr Ser Pro Phe His Cys Gln Cys Gly
705 710 715 720
Ser Asp Phe Cys Arg Val Arg Lys Gln Ile Ser Lys Gly Lys Asn Val
725 730 735
Lys Lys Arg Ala Lys Gly Gly Arg Ala Lys Leu Gly Thr Asp Tyr Lys
740 745 750
Asp Asp Asp Asp Lys Asp Tyr Lys Asp Asp Asp Asp Lys Asp Tyr Lys
755 760 765
Asp Asp Asp Asp Lys Gly Ser Leu Glu Val Leu Phe Gln Gly Pro Leu
770 775 780
Glu Pro Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe Ser Gln Ser Gly
785 790 795 800
Asp Leu Arg Arg His Gln Arg Thr His Thr Gly Glu Lys Pro Tyr Lys
805 810 815
Cys Pro Glu Cys Gly Lys Ser Phe Ser Arg Ser Asp His Leu Thr Thr
820 825 830
His Gln Arg Thr His Thr Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys
835 840 845
Gly Lys Ser Phe Ser Gln Ser Ser Ser Leu Val Arg His Gln Arg Thr
850 855 860
His Thr Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe
865 870 875 880
Ser Thr Ser Gly Asn Leu Val Arg His Gln Arg Thr His Thr Gly Glu
885 890 895
Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe Ser Gln Arg Ala
900 905 910
Asn Leu Arg Ala His Gln Arg Thr His Thr Gly Glu Lys Pro Tyr Lys
915 920 925
Cys Pro Glu Cys Gly Lys Ser Phe Ser Arg Ser Asp Lys Leu Thr Glu
930 935 940
His Gln Arg Thr His Thr Gly Leu Glu Gly Ser Met Ala Gly Gly Leu
945 950 955 960
Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu Asp Thr Gly
965 970 975
Gly Ser
<210> 79
<211> 888
<212> PRT
<213> 拟南芥
<400> 79
Met Gly Phe Val Tyr Ile Val Met Thr Gly Tyr Tyr Lys Asn Val His
1 5 10 15
Lys Arg Lys Gln Asn Gln Val Asp Asp Gly Pro Glu Ala Lys Arg Val
20 25 30
Lys Ser Ser Ala Lys Val Ile Asp Tyr Ser Asn Pro Phe Ala Val Ser
35 40 45
Asn Met Leu Glu Ala Leu Asp Ser Gly Lys Phe Gly Ser Val Ser Lys
50 55 60
Glu Leu Glu Glu Ile Ala Asp Met Arg Met Asp Leu Val Lys Arg Ser
65 70 75 80
Ile Trp Leu Tyr Pro Ser Leu Ala Tyr Thr Val Phe Glu Ala Glu Lys
85 90 95
Thr Met Asp Asn Gln Gln Val Val Glu Gly Val Ile Asn Leu Asp Asp
100 105 110
Asp Asp Asp Asp Asp Thr Asp Val Glu Lys Lys Ala Leu Cys Val Val
115 120 125
Pro Ser Ser Ser Glu Ile Val Leu Leu Asp Ser Asp Asp Glu Asp Asn
130 135 140
Glu Arg Gln Arg Pro Met Tyr Gln Phe Gln Ser Thr Leu Val Gln His
145 150 155 160
Gln Lys Asn Gln Gly Asp Val Thr Pro Leu Ile Pro Gln Cys Ser Phe
165 170 175
Glu Glu Val Asp Leu Gly Arg Gly Lys Glu Met Pro Ser Ala Ile Lys
180 185 190
Ala Ile Val Glu Gly Gln Thr Ser Arg Gly Lys Val Leu Pro Ile Glu
195 200 205
Asn Gly Val Val Asn Glu Lys Gly Val Tyr Val Gly Val Glu Glu Asp
210 215 220
Asp Ser Asp Asn Glu Ser Glu Ala Ala Asp Glu Asp Leu Gly Asn Ile
225 230 235 240
Trp Asn Glu Met Ala Leu Ser Ile Glu Cys Ser Lys Asp Val Ala Arg
245 250 255
Glu Thr Ser His Lys Glu Lys Ala Asp Val Val Glu Asp Cys Glu His
260 265 270
Ser Phe Ile Leu Lys Asp Asp Met Gly Tyr Val Cys Arg Val Cys Gly
275 280 285
Val Ile Glu Lys Ser Ile Leu Glu Ile Ile Asp Val Gln Phe Thr Lys
290 295 300
Ala Lys Arg Asn Thr Arg Thr Tyr Ala Ser Glu Thr Arg Thr Lys Arg
305 310 315 320
Phe Gly Glu Ser Asp Asn Glu Leu Lys Phe Ser Glu Glu Gly Leu Met
325 330 335
Ile Gly Gly Leu Ala Ala His Pro Thr His Ala Ala Glu Met Lys Pro
340 345 350
His Gln Ile Glu Gly Phe Gln Phe Leu Cys Ser Asn Leu Val Ala Asp
355 360 365
Asp Pro Gly Gly Cys Ile Met Ala His Ala Pro Gly Ser Gly Lys Thr
370 375 380
Phe Met Ile Ile Ser Phe Met Gln Ser Phe Leu Ala Lys Tyr Pro Gln
385 390 395 400
Ala Lys Pro Leu Val Val Leu Pro Lys Gly Ile Leu Pro Thr Trp Lys
405 410 415
Lys Glu Phe Val Arg Trp Gln Val Glu Asp Ile Pro Leu Leu Asp Phe
420 425 430
Tyr Ser Ala Lys Ala Glu Asn Arg Ala Gln Gln Leu Ser Ile Leu Lys
435 440 445
Gln Trp Met Glu Lys Lys Ser Ile Leu Phe Leu Gly Tyr Gln Gln Phe
450 455 460
Ser Thr Ile Val Cys Asp Asp Thr Thr Asp Ser Leu Ser Cys Gln Glu
465 470 475 480
Ile Leu Leu Lys Val Pro Ser Ile Leu Ile Leu Asp Glu Gly His Thr
485 490 495
Pro Arg Asn Glu Asp Thr Asn Leu Leu Gln Ser Leu Ala Gln Val Gln
500 505 510
Thr Pro Arg Lys Val Val Leu Ser Gly Thr Leu Tyr Gln Asn His Val
515 520 525
Lys Glu Val Phe Asn Ile Leu Asn Leu Val Arg Pro Lys Phe Leu Lys
530 535 540
Leu Asp Thr Ser Lys Ser Ala Val Lys Arg Ile Leu Ala Tyr Thr Pro
545 550 555 560
Cys Asp Val Arg Gly Arg Leu Thr Gly Ser Asn Ser Asp Met Ala Ser
565 570 575
Met Phe Asn Glu Thr Val Glu His Thr Leu Gln Lys Ser Glu Asp Phe
580 585 590
Thr Val Lys Ile Lys Val Ile Gln Asp Leu Arg Glu Met Thr Lys Lys
595 600 605
Val Leu His Tyr Tyr Lys Gly Asp Phe Leu Asp Glu Leu Pro Gly Leu
610 615 620
Ala Asp Phe Thr Val Val Leu Asn Leu Ser Pro Lys Gln Leu Asn Glu
625 630 635 640
Val Lys Lys Leu Arg Arg Glu Lys Arg Lys Phe Lys Val Ser Ala Val
645 650 655
Gly Ser Ala Ile Tyr Leu His Pro Lys Leu Lys Val Phe Ser Asp Lys
660 665 670
Ser Asp Asp Val Ser Asp Thr Thr Met Asp Glu Met Val Glu Lys Leu
675 680 685
Asp Leu Asn Glu Gly Val Lys Ala Lys Phe Phe Leu Asn Leu Ile Asn
690 695 700
Leu Cys Asp Ser Ala Gly Glu Lys Leu Leu Val Phe Ser Gln Tyr Leu
705 710 715 720
Ile Pro Leu Lys Phe Leu Glu Arg Leu Ala Ala Leu Ala Lys Gly Trp
725 730 735
Lys Leu Gly Lys Glu Val Phe Val Leu Thr Gly Asn Thr Ser Ser Glu
740 745 750
Gln Arg Glu Trp Ser Met Glu Thr Phe Asn Ser Ser Pro Asp Ala Lys
755 760 765
Ile Phe Phe Gly Ser Ile Lys Ala Cys Gly Glu Gly Ile Ser Leu Val
770 775 780
Gly Ala Ser Arg Ile Leu Ile Leu Asp Val Pro Leu Asn Pro Ser Val
785 790 795 800
Thr Arg Gln Ala Ile Gly Arg Ala Phe Arg Pro Gly Gln Lys Lys Met
805 810 815
Val His Ala Tyr Arg Leu Ile Ala Gly Ser Ser Pro Glu Glu Glu Asp
820 825 830
His Asn Thr Cys Phe Lys Lys Glu Val Ile Ser Lys Met Trp Phe Glu
835 840 845
Trp Asn Glu Tyr Cys Gly Tyr Gln Asn Phe Glu Val Glu Thr Ile Asp
850 855 860
Val Asp Glu Ala Gly Asp Thr Phe Leu Glu Ser Pro Ala Leu Arg Glu
865 870 875 880
Asp Ile Arg Val Leu Tyr Lys Arg
885
<210> 80
<211> 1157
<212> PRT
<213> 蓖麻
<400> 80
Met His Tyr Val Glu Lys Lys Arg Leu Ala Asn Gln Lys Thr Thr Ser
1 5 10 15
Val Pro Gln Cys Asp Val Ile Asn Leu Glu Asp Asp Pro Pro Gln Ser
20 25 30
His Val Ile Ala Ala Asp Ile Pro Val Ile Ile Leu Asp Ser Asp Glu
35 40 45
Glu Asp Asn Gly Tyr Gln Lys Pro Ser His Met Tyr Ser Lys Asn Ser
50 55 60
Ser Ser Glu Pro Ala His Lys Leu Ser Val Thr Val Ser Ala Val Lys
65 70 75 80
Asp Cys His Glu Val Arg Ala Ser Trp Asp Glu Thr Arg Ser Ala Pro
85 90 95
Ala Asn Asn Asp Phe Leu Val Asp Lys Arg Lys Asn Thr Asp Thr Lys
100 105 110
Asn Asp Ala Gln Val Lys Asp Thr Glu Lys Val Lys Ala Ser Arg Asp
115 120 125
Phe Val Gly Gly Ser Leu Ala Glu Cys Asn Ile His Ala Asn Glu Ser
130 135 140
Pro Tyr Pro Gly Met Lys Thr Asp Val Gln Val Gly Asp Ser Glu Asn
145 150 155 160
Val Lys Asn Ser Ala Ser Val Ile Val Asp Leu Leu Gly Lys Cys Lys
165 170 175
Ile Gln Val Asp Ser Asn Ser Tyr Ala Cys Met Asp Asn Asp Ile Val
180 185 190
Ile Lys Glu Asp Ser His Gln Asp Asp Ile Glu Asp Asp Gly Leu Val
195 200 205
Thr Ile Asp Thr Asp Val Asn Asn Phe Glu Asn Asp Arg Ser Gln Gly
210 215 220
His Val Thr Ala Ala Asp Ile Gln Val Ile Ile Ile Asn Ser Asp Glu
225 230 235 240
Glu Asp Ser Gly Tyr Gln Arg His Ser Tyr Leu Tyr Glu Asn Gly Thr
245 250 255
Leu Ser Gly Pro Ala His Lys Leu Pro Val Lys Asp Leu Thr Val Thr
260 265 270
Asp Cys Gln Gly Val Lys Asp Pro Lys Asp Glu Ala Arg His Ser Pro
275 280 285
Ala Asn Tyr Cys Phe Gln Val Asn Lys Asn Glu Tyr Thr Asp Thr Lys
290 295 300
Asn Asp Ile Gln Val Glu Asp Asn Glu Asn Val Lys Ala Ser Lys Cys
305 310 315 320
Asp Val Gly Gly Ser Leu Ala Glu Cys Asn Ile Arg Val Asp Glu Ile
325 330 335
Pro His Ser Gly Met Glu Ser Ile Val Gln Val Arg Asn Ser Lys Lys
340 345 350
Val Lys Asp Pro Cys Asp Val Ile Gly Asp Ser His Gly Lys Cys Ser
355 360 365
Ile Phe Ala Asp Val Asn Ser Tyr Thr Ser Met Lys Ser Thr Ala Ala
370 375 380
Ile Lys Lys Glu Asn His Gln Ile Gly Val Glu Asp Asp Ala Ile Val
385 390 395 400
Glu Asn Glu Thr Glu Asn Asp Ile Gln Ile Lys Asp Asn Lys Arg Asp
405 410 415
Lys Ala Ser Arg Ile Thr Ile Gly Asn Ser Pro Thr Lys Cys Ser Thr
420 425 430
Gln Val Leu Tyr Ala Thr Met Glu Asn His Met Val Ile Lys Glu Asp
435 440 445
Ile His Gln Ile Asn Ala Lys Asp Asn Gly Leu Ala Lys Ile Asp Thr
450 455 460
Glu Gly Asp Ile Gln Asp Met Asp Ser Lys Glu Phe Lys Ala Ser Arg
465 470 475 480
Asp Thr Val Glu Gly Ser Leu Asp Lys Cys Asn Ile Gln Val Asp Lys
485 490 495
Arg Pro Tyr Ala Val Met Lys Ile Ser Met Ala Ile Lys Glu Asp Ser
500 505 510
Gln His Ile Ser Val Glu Asp Asn Val Leu Ser Lys Ile Asp Thr Glu
515 520 525
Asp Asp Ile Gln Asp Thr Val Gly Lys Arg Val Lys Ala Leu Ser Ile
530 535 540
Lys Val Asp Asp Ser Met Tyr Thr Asp Met Glu Asn Ala Met Ala Ile
545 550 555 560
Glu Glu Asp Asn Arg Gln Ile Asp Val Glu Asp Asp Gly Leu Ala Asp
565 570 575
Ile Trp Lys Glu Met Lys Phe Ala Ile Glu Tyr Ser Lys Asp Phe Ala
580 585 590
Val Asp Pro Ala Thr Tyr Gly Cys Leu Glu Glu Asp Ala Glu Ala Cys
595 600 605
Asp His Ser Phe Val Leu Lys Asp Asp Leu Gly Tyr Val Cys Arg Ile
610 615 620
Cys Gly Ile Ile Gln Arg Ser Ile Asp Thr Ile Phe Glu Phe Gln Tyr
625 630 635 640
Ala Lys Lys Arg Asn Met Arg Thr Tyr Ala Tyr Glu Pro Lys Tyr Thr
645 650 655
Ile Gly Lys Glu Pro Ile Gly Gly Ile Pro Val Gly Ile Asn Ser Pro
660 665 670
Asp Leu Met Val Thr Glu Ile Ala Ala His Pro Arg His Lys Lys Glu
675 680 685
Met Lys Pro His Gln Val Glu Gly Phe Asn Phe Leu Cys Ser Asn Leu
690 695 700
Val Gly Asp Asn Pro Gly Gly Cys Ile Leu Ala His Ala Pro Gly Ser
705 710 715 720
Gly Lys Thr Phe Met Ile Ile Thr Phe Ile Gln Ser Phe Leu Ala Lys
725 730 735
Tyr Pro Asn Ala Arg Pro Met Val Val Leu Pro Lys Gly Ile Leu Ala
740 745 750
Thr Trp Lys Lys Glu Phe Glu Lys Trp Gln Val Glu Asn Ile Pro Leu
755 760 765
His Asp Phe Tyr Thr Leu Lys Ala Glu Asn Arg Ser Gln Gln Leu Asp
770 775 780
Val Leu Lys Arg Trp Val Lys Glu Arg Ser Ile Leu Phe Leu Gly Tyr
785 790 795 800
Lys Gln Leu Ser Thr Ile Val Cys Asp Ser Ser Lys Val Ala Ala Glu
805 810 815
Cys Gln Asp Ile Leu Leu Lys Val Thr Leu Arg Glu Met Arg Arg Leu
820 825 830
Met Tyr Phe Asn Pro Ser Gln Lys Phe Lys Pro Leu Phe Leu Lys Leu
835 840 845
Lys Ala Ser Arg Met Ile Ile Asn Arg Ile Met Gly Arg Val Gln Ile
850 855 860
Ser Arg Val Arg Lys His Leu Lys His Gly Gly Asp Ser Ala Phe Phe
865 870 875 880
Asp Ser Val Glu Tyr Thr Leu Gln Lys Asp Glu Asp Phe Lys Arg Lys
885 890 895
Lys Ala Val Ile Gln Asp Leu Arg Gln Met Thr Ser Lys Val Leu His
900 905 910
Tyr Tyr Lys Gly Asp Phe Leu Asp Gln Phe Pro Gly Leu Val Asp Phe
915 920 925
Thr Val Val Leu Asn Leu Ser Ala Arg Gln Lys Asp Glu Val Gln Lys
930 935 940
Leu Arg Lys Leu Asp Lys Phe Lys Arg Ile Ser Leu Gly Ser Ala Val
945 950 955 960
Tyr Met His Pro Arg Leu Lys Asp Phe Ser Gln Val Cys Phe Ala Thr
965 970 975
Arg Glu Lys Gly Ser Asn Ile Asp Asp Arg Thr Ile Asp Ala Leu Leu
980 985 990
Gly Asn Leu Asp Val Arg Glu Gly Val Lys Thr Lys Phe Phe Leu Asn
995 1000 1005
Ile Leu Arg Leu Cys Glu Ser Ala Gly Glu Lys Leu Leu Val Phe Ser
1010 1015 1020
Gln Tyr Ile Leu Pro Leu Lys Leu Leu Glu Arg Leu Ser Ala Lys Val
1025 1030 1035 1040
Lys Gly Trp Ser Leu Gly Lys Glu Leu Phe Met Ile Thr Gly Asp Ser
1045 1050 1055
Asn Thr Glu Gln Arg Glu Ile Cys Met Glu His Phe Asn Asp Ser Pro
1060 1065 1070
Ser Ala Lys Val Phe Phe Gly Ser Ile Lys Ala Cys Gly Glu Gly Ile
1075 1080 1085
Ser Leu Glu Asp Tyr Leu Thr Cys Ser Arg Lys Glu Leu Ile Ser Lys
1090 1095 1100
Met Trp Phe Glu Trp Asn Glu His Cys Gly Asp Gln Asp Phe Glu Val
1105 1110 1115 1120
Lys Thr Thr Asp Val Asn Glu Cys Gly Asp Met Phe Phe Glu Ser Pro
1125 1130 1135
Gly Leu Arg Glu Asp Leu Asn Val Leu Tyr Lys Arg Lys Arg Arg Gln
1140 1145 1150
Arg Gly Thr Leu Lys
1155
<210> 81
<211> 883
<212> PRT
<213> 大豆
<400> 81
Met Glu Ser Ala Asp Asp Val Ser Ala Ser Lys Arg Pro Leu Pro Thr
1 5 10 15
Gly Phe Ser Pro Gly Pro Asp Gly Pro Lys Arg Leu Arg Leu Ser Ser
20 25 30
Asp Ser Leu Pro Tyr Pro Ala Cys Asp Gln Ile Glu Pro Lys Thr Pro
35 40 45
Lys Asn Pro Asn Val Val Asp Tyr Ser Asn Pro Phe Ala Ile Thr Asp
50 55 60
Ile Leu Asp Arg Leu Glu Asn Gly Lys Phe Gly Ser Val Thr Lys Asp
65 70 75 80
Ile Glu Ala Leu Ile Ala Leu Lys Met Gln Ile Met Gly Pro Tyr Phe
85 90 95
Ala Lys Tyr Pro Ile Leu Val Asn Gln Leu Leu Lys Val Val Thr His
100 105 110
His Asp Glu Glu Thr Pro Lys Leu Glu Asn Gln Gln Val Thr Gly Leu
115 120 125
Thr His Gln Ser Val Ile Asp Leu Glu Gly Glu Tyr Thr Glu Lys Asp
130 135 140
Val Pro Ala Val Pro Asn Thr Val Val Ile Ile Asp Ser Asp Glu Glu
145 150 155 160
Asp Asp Arg Asp Lys Lys Ser Val Ile Pro Phe His Glu Val Val Leu
165 170 175
Pro Arg Lys Val Ala Pro Ser Pro Ala Leu Lys Val Ile Gly Tyr His
180 185 190
Thr Tyr Leu Gly Glu Ser Asn Asp Leu Lys Ile Glu Ile Asn Met Ala
195 200 205
Asp Lys Gly Asn Asn Thr Arg Ser Asn Lys Gly Val Tyr Val Gly Ala
210 215 220
Gln Gly Glu Glu Glu Asp Lys Ala Asp Thr Glu Asp Asp Gly Leu Gln
225 230 235 240
Asp Ile Trp Lys Glu Met Ser Met Ala Ile Glu Cys Ser Lys Asp Val
245 250 255
Ser Glu Asp Pro Glu Pro Glu Glu Glu Glu Glu Glu Asp Asp Asn Cys
260 265 270
Asp His Ser Phe Val Leu Lys Asp Asp Leu Gly Tyr Val Cys Arg Val
275 280 285
Cys Gly Val Ile Asp Arg Lys Ile Glu Thr Ile Phe Glu Phe Gln Tyr
290 295 300
Lys Val Lys Arg Ser Thr Arg Thr Tyr Ala Ser Asp Ser Trp Asn Ser
305 310 315 320
Lys Gly Lys Ala Asp Val Phe Gly Ile Asn Val Ala Glu Asp Asp Leu
325 330 335
Val Val Thr Asp Ile Ala Ala His Pro Arg His Met Lys Gln Met Lys
340 345 350
Pro His Gln Val Glu Gly Phe Asn Phe Leu Val Arg Asn Leu Ala Gly
355 360 365
Asp His Pro Gly Gly Cys Ile Leu Ala His Ala Pro Gly Ser Gly Lys
370 375 380
Thr Phe Met Ile Ile Ser Phe Met Gln Ser Phe Leu Gly Lys Tyr Pro
385 390 395 400
Asn Ala Arg Pro Leu Val Val Leu Pro Lys Gly Ile Leu Ser Thr Trp
405 410 415
Lys Lys Glu Phe Gln Thr Trp Gln Val Glu Asp Ile Pro Leu Tyr Asp
420 425 430
Leu Tyr Thr Val Lys Ala Asp Ser Arg Ser Gln Gln Leu Glu Val Leu
435 440 445
Lys Gln Trp Met Glu Gln Lys Ser Ile Leu Phe Leu Gly Tyr Lys Gln
450 455 460
Phe Ser Ser Ile Val Cys Asp Asn Gly Thr Asn Asn Thr Ser Leu Ser
465 470 475 480
Cys Gln Glu Ile Leu Leu Lys Ile Pro Thr Ile Leu Ile Leu Asp Glu
485 490 495
Gly His Asn Pro Arg Asn Glu Asn Thr Asp Met Val Gln Ser Leu Ala
500 505 510
Lys Val Gln Thr Ala Arg Lys Val Val Leu Ser Gly Thr Leu Tyr Gln
515 520 525
Asn His Val Arg Glu Val Phe Asn Ile Leu Asn Leu Val Arg Pro Lys
530 535 540
Phe Leu Lys Met Glu Thr Ser Arg Pro Ile Val Arg Arg Ile His Ser
545 550 555 560
Arg Val His Ile Pro Gly Val Arg Ser Phe Tyr Asp Leu Val Glu Asn
565 570 575
Thr Leu Gln Lys Asp Thr Asp Phe Lys Arg Lys Ile Ala Val Ile Gln
580 585 590
Asp Leu Arg Glu Met Thr Ser Lys Val Leu His Tyr Tyr Lys Gly Asp
595 600 605
Phe Leu Asp Glu Leu Pro Gly Leu Val Asp Phe Thr Val Val Leu Thr
610 615 620
Leu Ser Pro Arg Gln Lys Pro Glu Ile Gln Lys Leu Lys Lys Leu Ser
625 630 635 640
Arg Arg Lys Phe Lys Ile Asn Ser Val Gly Ser Ala Val Tyr Leu His
645 650 655
Pro Lys Leu Lys Pro Leu Ala Glu Asn Cys Gly Glu Asn Ser Thr Ser
660 665 670
Asp Asn Ile Met Asp Asp Leu Ile Glu Lys Leu Asp Met Arg Asp Gly
675 680 685
Val Lys Ser Lys Phe Tyr Tyr Asn Met Leu Asn Leu Cys Glu Ser Ala
690 695 700
Gly Glu Lys Leu Leu Val Phe Ser Gln Tyr Leu Leu Pro Leu Lys Tyr
705 710 715 720
Leu Glu Arg Leu Thr Met Lys Trp Lys Gly Trp Ser Leu Gly Arg Glu
725 730 735
Ile Phe Val Ile Ser Gly Glu Ser Ser Ser Glu Gln Arg Glu Trp Ser
740 745 750
Met Glu Lys Phe Asn Asn Ser Pro Asp Ala Arg Val Phe Phe Gly Ser
755 760 765
Ile Lys Ala Cys Gly Glu Gly Ile Ser Leu Val Gly Ala Ser Arg Ile
770 775 780
Ile Ile Leu Asp Val His Leu Asn Pro Ser Val Thr Arg Gln Ala Ile
785 790 795 800
Gly Arg Ala Phe Arg Pro Gly Gln Met Lys Lys Val Phe Val Tyr Arg
805 810 815
Leu Val Ser Ala Asp Ser Pro Glu Glu Glu Asp His Asn Thr Cys Phe
820 825 830
Lys Lys Glu Leu Ile Ser Lys Met Trp Phe Glu Trp Asn Glu Tyr Cys
835 840 845
Gly Asp Arg Ala Phe Glu Val Glu Ala Val Glu Val Lys Glu Cys Gly
850 855 860
Asp Leu Phe Leu Glu Ser Pro Leu Leu Gly Glu Asp Val Lys Ala Leu
865 870 875 880
Tyr Lys Arg
<210> 82
<211> 950
<212> PRT
<213> 玉蜀黍
<400> 82
Met Ser Gln Ser Pro Gly Gly Arg Glu Gly Ile Tyr Tyr Ser Arg Gln
1 5 10 15
Arg Lys Pro Ser Glu Arg Asn Gly Ser Val Phe Thr Pro Ile Ala Ala
20 25 30
Met Tyr Pro Ser Gly His Ala Leu Pro Asp Ala Asn Arg Asn His Ser
35 40 45
Leu Val Phe Gly Gly Thr Ser Lys Asp Trp Asp Asn Ile Arg Gln Phe
50 55 60
Ile Ala Ser Leu Glu Arg Ala Ser Glu Asn Ser Ser Ala Ile Ala Ser
65 70 75 80
Lys Thr Gly Gly Gly Lys Ser Thr Asn His Ser Val Glu Pro Ala Glu
85 90 95
Gln Lys Gly Lys Gly Asp Ile Ile Val Leu Asp Ser Asp Asp Glu Asp
100 105 110
Gly Asp Gly Asn Ser Pro Glu His Asn Lys Leu Ala Ser Glu Met Asn
115 120 125
Lys Glu Leu Gly Thr Ser Val Leu Ala Ser Asn Ile Ala Glu Arg Met
130 135 140
Ala Thr Asn Gly Ser Gln Thr Phe Glu Thr Val His Ala Tyr Gly Gly
145 150 155 160
Ser Lys Asn Thr Gln Ile Val Pro Tyr Gly Gln Gly Ser Ala Leu Val
165 170 175
Asn Gln Phe Pro Leu Gln Thr Ser Trp Gln Pro Ser Ile Gln Phe Glu
180 185 190
Arg Val Val Leu Thr Lys Arg Pro Glu Glu Gln Arg Met Gln Asp Leu
195 200 205
Val Ala Ala Thr Ile Ala Glu Lys Arg Ala Glu Thr Gln Met Phe Leu
210 215 220
Ser Leu Pro Thr Glu Arg Lys Arg Arg Arg Thr Asp His Ser Leu Leu
225 230 235 240
Met Leu Asp Ser Phe Val Pro Lys Gln Arg Arg Arg Lys Gly Asp Thr
245 250 255
Gly Leu Ala Pro Ala Asp Leu Ser Leu Asp Leu His Gln Thr Ala Thr
260 265 270
Ser Gln Glu Pro Asp Ile Ala Ile Glu Glu Glu Glu Lys Arg Lys Asn
275 280 285
Asp Gly Asp Gly Leu Glu Asp Tyr Trp Lys Asp Phe Ala Leu Ala Val
290 295 300
Glu Ser Thr Lys Leu Asp Asp Val Asp Glu Ala Ala Ala Asn Glu Lys
305 310 315 320
Glu Asp Asn Gly Lys Met Glu Asp Ile Asp Cys Asn His Asp Ile Arg
325 330 335
Ile His Glu Asp Leu Gly His Val Cys Arg Val Cys Gly Met Ile Val
340 345 350
Arg Arg Ala Asp Ser Ile Ile Asp Tyr Gln Trp Lys Lys Ala Ser Arg
355 360 365
Arg Arg Met Asn Gly Tyr Gly Gly Asn Ser Lys Asp Ala Asp Glu Ile
370 375 380
Asp Cys Gly Thr Val Lys Leu Ser Glu Asp Phe Ile Val Ala Asp Ile
385 390 395 400
Ala Ile His Pro Arg His Ala Gln Ala Met Lys Pro His Gln Val Glu
405 410 415
Gly Phe Asn Phe Leu Val Lys Asn Leu Ile Gly Asp Lys Pro Gly Gly
420 425 430
Cys Ile Leu Ala His Ala Pro Gly Ser Gly Lys Thr Phe Leu Leu Ile
435 440 445
Ser Phe Ile Gln Ser Phe Met Ala Arg Tyr Pro Ser Ala Arg Pro Leu
450 455 460
Val Val Leu Pro Lys Gly Ile Leu Val Ile Trp Lys Lys Glu Ile Gln
465 470 475 480
Arg Trp Gln Val Gln Asp Ile Pro Val Tyr Asp Phe Tyr Ser Val Lys
485 490 495
Ala Glu Lys Arg Val Glu Gln Leu Gln Ile Leu Lys Ser Trp Glu Asp
500 505 510
Lys Met Gly Ile Leu Phe Leu Gly Tyr Lys Gln Phe Ser Thr Ile Val
515 520 525
Thr Asp Asp Gly Gly Ser Lys Val Thr Ala Ala Cys Arg Asp Arg Leu
530 535 540
Leu Lys Val Pro Asn Leu Leu Ile Leu Asp Glu Gly His Thr Pro Arg
545 550 555 560
Asn Lys Glu Thr Asp Val Leu Glu Ser Leu Ser Arg Val Glu Thr Pro
565 570 575
Arg Lys Val Val Leu Ser Gly Thr Leu Phe Gln Asn His Val Glu Glu
580 585 590
Val Phe Asn Ile Leu Asn Leu Val Arg Pro Lys Phe Leu Arg Met Glu
595 600 605
Ser Ser Arg Pro Ile Ala Arg Arg Ile Met Ser Gln Val Glu Ile Phe
610 615 620
Gly Arg Ser Ser Lys Gly Leu Ala Asp Gly Ala Phe Thr Glu Ala Val
625 630 635 640
Glu Gly Thr Leu Leu Asn Asp Glu Asn Phe Lys Arg Lys Val His Val
645 650 655
Ile Arg Gly Leu Arg Glu Leu Thr Arg Asp Val Leu His Tyr Tyr Lys
660 665 670
Gly Ala Ile Leu Asp Glu Leu Pro Gly Leu Val Asp Phe Ser Val Phe
675 680 685
Leu Lys Leu Thr Pro Lys Gln Lys Asp Ile Val His Lys Leu Glu Met
690 695 700
His Asp Arg Phe Lys Arg Ser Ala Val Gly Ser Ala Leu Tyr Ile His
705 710 715 720
Pro Cys Leu Ser Gly Leu Ser Glu Val Asn Ala Glu Asn Arg Ala His
725 730 735
Thr Leu Arg Asp Asp Ser Val Asp Ser Leu Met Asp Ser Ile Asn Val
740 745 750
Arg Asp Gly Val Lys Ala Asn Phe Phe Met Asn Ile Leu Ser Leu Ala
755 760 765
Asn Ser Ala Gly Glu Lys Val Leu Ala Phe Ser Gln Tyr Ile Leu Pro
770 775 780
Met Thr Phe Phe Glu Arg Leu Leu Val Lys Lys Lys Gly Trp His Val
785 790 795 800
Gly Arg Glu Ile Phe Met Ile Ser Gly Asp Thr Ser Gln Glu Asp Arg
805 810 815
Glu Ala Ala Val Asp Arg Phe Asn Ser Ser Ala Asp Ala Lys Val Leu
820 825 830
Phe Gly Ser Ile Arg Ala Cys Gly Glu Gly Ile Ser Ile Val Gly Ala
835 840 845
Ser Arg Val Val Ile Leu Asp Val His Leu Asn Pro Ser Val Thr Arg
850 855 860
Gln Ala Ile Gly Arg Ala Phe Arg Pro Gly Gln Gln Lys Lys Val Phe
865 870 875 880
Val Tyr Arg Leu Val Ala Ala Asp Ser Asp Glu Val Lys Val His Glu
885 890 895
Thr Ala Phe Lys Lys Glu Val Ile Gln Lys Leu Trp Phe Glu Trp Ser
900 905 910
Glu Gln Cys Thr Thr Glu Asn Phe Lys Leu Gly Gln Val Asp Ile Asp
915 920 925
Asp Ser Gly Asp Glu Leu Leu Asp Thr Arg Ala Ile Arg Gln Asp Ile
930 935 940
Lys Ala Leu Tyr Arg Arg
945 950
<210> 83
<211> 653
<212> PRT
<213> 小立碗藓
<400> 83
Met Asn Glu Ile Asp Arg Ala Phe Cys Ile Asn Thr Leu Thr Ser Trp
1 5 10 15
Arg Thr Phe Cys Val Gln Gly Lys Gly Glu Val Asn Val Glu Glu Val
20 25 30
Thr Glu Ser Cys Glu His Val Tyr Glu Val Lys Asn Asp Val Gly Arg
35 40 45
Ala Cys Ser Gln Cys Gly Leu Ile Lys Val Asp Ile Lys Asp Met Thr
50 55 60
Phe Met Trp Arg Arg Pro Cys Ser Val Arg Lys Ala Phe Val Arg Gly
65 70 75 80
Glu Arg Leu Asp Asn Trp Asn Asp Ser Gly Asp Gly Val Leu Glu Glu
85 90 95
Asp Val Arg Leu Val Gly Met Ala Gln Leu Glu Ala His Pro Ser Leu
100 105 110
Glu Asn Ala Met His Pro His Gln Leu Glu Gly Phe Lys Phe Leu Ser
115 120 125
Arg Asn Leu Val Glu Glu Asp Ser Gly Gly Cys Met Leu Ala Phe Ala
130 135 140
Pro Gly Thr Gly Lys Ser Phe Leu Ile Ile Ser Phe Ile Gln Ser Phe
145 150 155 160
Met Val Gln Val Pro Asn Ala Arg Pro Met Ile Val Ala Pro Lys Ser
165 170 175
Met Leu Arg Pro Trp Met Gln Glu Phe Lys Lys Trp Glu Val Glu Glu
180 185 190
Met Leu Val Leu Asn Leu Tyr Glu Ala Asp Asp His Val Lys Met Leu
195 200 205
Lys Gln Trp Met Ala Thr Pro Ser Val Leu Leu Val Gly Tyr Ser Gln
210 215 220
Phe Ile Asn Pro Ser Gly Glu Val Gly Lys Tyr Leu Thr Glu Gly Pro
225 230 235 240
Gly Met Ile Val Met Asp Glu Gly His Leu Ala Arg Thr Glu Glu Thr
245 250 255
Lys Ile Leu Lys Ser Leu Thr Arg Val His Thr Arg Arg Arg Val Leu
260 265 270
Leu Ser Gly Thr Pro Phe Asn Asn Asn Phe Glu Glu Phe Tyr Thr Thr
275 280 285
Leu Glu Leu Val Arg Pro Asn Phe Met Ser Arg Ala Asn Ala Gln Met
290 295 300
Phe Pro Thr Leu Asn Thr Phe Gln Gln Ile Val Asp Thr Lys Met Asn
305 310 315 320
Ser Lys Gln Pro Val Leu Asp Gly Gln Leu Leu Lys Arg Ser Thr Asn
325 330 335
Ala Gly Arg Lys Ala Phe Gln Asp Val Ile Gly Glu Lys Phe Glu Ser
340 345 350
Gly Lys Tyr Ser Asn Ile Ala Lys Ala Leu Gln Gln Leu Arg Val Leu
355 360 365
Ile Glu Pro Phe Val Ala Trp His Lys Gly Gln Ile Leu Asn Ser Leu
370 375 380
Pro Gly Ile Thr Asp Leu Thr Ile Met Leu Glu Leu Thr Ala Gln Gln
385 390 395 400
Leu Glu Leu Val Arg Lys Asn Lys Gln Asn Glu Val Arg Asp Ser Leu
405 410 415
Gln Lys Arg Ala Ala Ala Ile Tyr Val His Pro Ile Leu Glu Pro Val
420 425 430
Ala Asp Gly Val Lys Arg Ser Gln Arg Asp Pro Arg Leu Lys Gly Asp
435 440 445
Val Asp Val Lys Ala Gly Ala Lys Leu Arg Trp Val Leu Asp Met Val
450 455 460
Gln Gln Cys Asn Asp Ala Lys Glu Lys Leu Leu Ile Phe Ser Glu Tyr
465 470 475 480
Leu Tyr Ser Leu Ala Leu Ile Glu Asn Met Ala Val Gln Arg Met Lys
485 490 495
Trp Ser Lys Gly Leu Gln Ile Leu Arg Leu Asp Gly Ser Leu Pro Pro
500 505 510
Gln Glu Arg Glu Arg Val Gln His Lys Phe Asn Thr Asp Pro Glu Val
515 520 525
Arg Val Leu Cys Ala Ser Ile Lys Ala Cys Gly Glu Gly Ile Ser Leu
530 535 540
Val Gly Ala Ser Arg Val Ile Leu Leu Glu Val Leu Trp Asn Pro Ser
545 550 555 560
Val Pro Arg Gln Ala Ile Ser Arg Ala Phe Arg Ile Gly Gln Gln Arg
565 570 575
Lys Val Val Val Tyr Arg Leu Ile Ala Ala Gly Thr Tyr Glu Glu Met
580 585 590
Asn Met His Ala Ala Ala Thr Arg Lys Glu Trp Leu Ser Arg Leu Leu
595 600 605
Phe Asp Pro Thr Ile Pro Cys Asp Asp Pro Asn Ser Ile Leu Trp Asp
610 615 620
Val Thr Lys Asp Cys Ser Asp Ser Phe Leu Ala Tyr Lys Gln Ser Lys
625 630 635 640
Leu Arg Glu Gly Val Arg Ser Ile Tyr Gln Arg Glu Phe
645 650
<210> 84
<211> 748
<212> PRT
<213> 两色蜀黍
<400> 84
Met Gln Asp Leu Val Ala Ala Ser Ile Ala Glu Lys Arg Ala Glu Thr
1 5 10 15
Gln Met Phe Leu Ser Leu Pro Thr Glu Lys Lys Arg Lys Arg Ser Asp
20 25 30
Leu Ser Leu Leu Met Leu Asp Ser Phe Val Pro Lys Gln Arg Arg Lys
35 40 45
Ser Asp Thr Gly Leu Ala Ala Ala Asp Leu Ser Leu Asp Leu Gln Gln
50 55 60
Pro Ala Lys Ser Pro Glu Pro Glu Pro Glu Pro Asp Met Ala Ile Glu
65 70 75 80
Glu Glu Glu Lys Cys Lys Asn Glu Ser Asp Gly Leu Glu Asp Tyr Trp
85 90 95
Lys Asp Phe Ala Leu Ala Val Glu Ser Thr Lys Leu Asp Glu Val Asp
100 105 110
Glu Ala Ala Asn Glu Lys Glu Asp Asn Gly Asn Met Glu Asp Ile Asp
115 120 125
Cys Asn His Asp Ile Arg Ile His Glu Asp Leu Gly His Val Cys Arg
130 135 140
Val Cys Gly Met Ile Val Arg Arg Ala Asp Ser Ile Ile Asp Tyr Gln
145 150 155 160
Trp Lys Lys Ala Ser Arg Arg Lys Thr Asn Ser Tyr Gly Gly His Ser
165 170 175
Lys Asp Ala Asp Glu Ile Asp Cys Gly Thr Val Lys Leu Ser Glu Asp
180 185 190
Phe Ile Ile Ala Asp Val Ala Ile His Pro Arg His Ala Gln Thr Met
195 200 205
Lys Pro His Gln Leu Glu Gly Phe Asn Phe Leu Val Lys Asn Leu Ile
210 215 220
Gly Asp Lys Pro Gly Gly Cys Ile Leu Ala His Ala Pro Gly Ser Gly
225 230 235 240
Lys Thr Phe Met Leu Ile Ser Phe Ile Gln Ser Phe Met Ala Arg Tyr
245 250 255
Pro Ser Ala Arg Pro Leu Val Val Leu Pro Lys Gly Ile Leu Gly Thr
260 265 270
Trp Lys Lys Glu Ile Gln Arg Trp Gln Val Gln Asp Ile Pro Leu Tyr
275 280 285
Asp Phe Tyr Ser Val Lys Ala Glu Lys Arg Val Glu Gln Leu Gln Ile
290 295 300
Leu Lys Ser Trp Glu Asp Lys Met Ser Ile Leu Phe Leu Gly Tyr Lys
305 310 315 320
Gln Phe Ala Thr Ile Val Thr Asp Asp Gly Gly Ser Asn Val Thr Ala
325 330 335
Ala Cys Arg Asp Arg Leu Leu Lys Val Pro Asn Leu Leu Ile Leu Asp
340 345 350
Glu Gly His Thr Pro Arg Asn Arg Glu Thr Asp Val Leu Glu Ser Leu
355 360 365
Asn Arg Val Glu Thr Pro Arg Lys Val Val Leu Ser Gly Thr Leu Phe
370 375 380
Gln Asn His Val Glu Glu Val Phe Asn Ile Leu Asn Leu Val Arg Pro
385 390 395 400
Lys Phe Leu Arg Met Glu Ser Ser Arg Pro Ile Ala Arg Arg Ile Met
405 410 415
Ser Gln Val Glu Ile Ser Gly Arg Gly Ser Lys Gly Phe Ala Asp Ser
420 425 430
Ala Phe Thr Glu Ala Val Glu Gly Thr Leu Leu Asn Asp Glu Asn Phe
435 440 445
Lys Arg Lys Ala His Val Ile Arg Gly Leu Arg Glu Leu Thr Lys Asp
450 455 460
Val Leu His Tyr Tyr Lys Gly Asp Ile Leu Asp Glu Leu Pro Gly Leu
465 470 475 480
Val Asp Phe Ser Val Phe Leu Lys Leu Thr Pro Lys Gln Lys Asp Ile
485 490 495
Ile Arg Asn Lys Leu Glu Ser His Asp Arg Phe Lys Arg Ser Ala Val
500 505 510
Gly Thr Ala Leu Tyr Ile His Pro Cys Leu Ser Gln Leu Ser Glu Val
515 520 525
Asn Ala Glu Asn Arg Ala Asn Thr Leu Arg Asp Asp Leu Val Asp Ser
530 535 540
Leu Leu Asp Ser Ile Asn Val Lys Asp Gly Val Lys Ala Asn Phe Phe
545 550 555 560
Met Asn Ile Leu Ser Leu Ala Asn Ser Ala Gly Glu Lys Val Leu Ala
565 570 575
Phe Ser Gln Tyr Ile Leu Pro Met Lys Phe Phe Glu Arg Leu Leu Val
580 585 590
Lys Met Lys Gly Trp His Val Gly Lys Glu Ile Phe Met Ile Ser Gly
595 600 605
Asp Thr Ser Gln Glu Asp Arg Glu Val Ala Val Asp His Phe Asn Asn
610 615 620
Ser Ala Asp Ala Lys Val Leu Phe Gly Ser Ile Lys Ala Cys Gly Glu
625 630 635 640
Gly Ile Ser Leu Val Gly Ala Ser Arg Val Val Ile Leu Asp Val His
645 650 655
Leu Asn Pro Ser Val Thr Arg Gln Ala Ile Gly Arg Ala Phe Arg Pro
660 665 670
Gly Gln Gln Lys Lys Val Phe Val Tyr Arg Leu Val Ala Ala Asp Ser
675 680 685
Asp Glu Glu Lys Val His Glu Thr Ala Phe Lys Glu Val Ile Pro Lys
690 695 700
Leu Trp Phe Glu Trp Ser Glu His Cys Thr Thr Glu Asp Phe Lys Leu
705 710 715 720
Gly Gln Val Asp Ile Asp Asp Ser Gly Asp Glu Leu Leu Asp Thr Lys
725 730 735
Ala Ile Arg Gln Asp Ile Lys Ala Leu Tyr Arg Arg
740 745
<210> 85
<211> 872
<212> PRT
<213> 稻
<400> 85
Met Asp Thr Pro Leu Glu Gln Lys Gln Lys Asp Ser Ser Ala Ile Ile
1 5 10 15
Val Leu Asp Ser Asp Asp Glu Asp Glu Ala Glu Arg Cys Glu Gln Leu
20 25 30
Ala Ser Glu Asn Asn Lys Gln Gln Ala Pro Ser Gly Pro Thr Ser Pro
35 40 45
Cys Thr Thr Trp Ile Val Ser Ser Ala Lys Asp Gln Val Asn Gly Thr
50 55 60
Leu His Val Asp Gly Val Gln Ser Thr Gln Ile Val Pro Tyr Gly Gln
65 70 75 80
Asn Ala Pro Leu Ile Asn Gln Ser Pro Leu Gln Thr Ser Trp Gln Pro
85 90 95
Ser Ile Gln Tyr Glu Arg Val Ile Leu Gln Arg Arg Pro Glu Glu Gln
100 105 110
Arg Val Gln Asp Leu Val Ala Ala Ser His Ala Glu Lys Ile Ala Glu
115 120 125
Thr Gln Val Phe Leu Thr Leu Pro Thr Leu Pro Asn Glu Arg Lys Arg
130 135 140
Arg Lys Ser Glu Pro Thr Thr Leu Val Asp Gly Asp Gly Gly Thr Asn
145 150 155 160
Leu Gly Lys Gly Lys Arg Lys Asn His Gln Asn Gln Ala Ala Val Asp
165 170 175
Ser Ile Leu Asp Leu Gln Gln Thr Val Val Pro Leu Gln Gln Asn Asp
180 185 190
Val Pro Ser Gln Ser Tyr Arg Thr Met Ile Glu Glu Glu Lys Pro Val
195 200 205
Lys Glu Ser Asp Gly Leu Glu Asp Leu Trp Lys Asp Phe Ser Leu Ala
210 215 220
Ala Glu Cys Thr Lys Leu Asp Thr Asn Glu Asp Met Ser Asn Glu Lys
225 230 235 240
Asp Val Asp Asp Glu Asn Glu Met Asp Asp Asp Cys Asn His Asp Ile
245 250 255
Arg Ile His Glu Asp Leu Gly His Val Cys Arg Ile Cys Gly Met Ile
260 265 270
Val Arg Lys Ala Glu Thr Ile Ile Asp Tyr Gln Trp Lys Lys Ala Ser
275 280 285
Arg Thr Arg Thr Asn Tyr Tyr Glu Ser Arg Ser Lys Asp Ala Asp Asp
290 295 300
Ile Asp Thr Gly Ala Val Lys Val Ser Glu Asp Phe Ile Val Ser Asp
305 310 315 320
Ile Ala Ile His Pro Arg His Ala Lys Gln Met Arg Pro His Gln Leu
325 330 335
Glu Gly Phe Ser Phe Leu Val Lys Asn Leu Val Gly Asp Lys Pro Gly
340 345 350
Gly Cys Ile Leu Ala His Ala Pro Gly Ser Gly Lys Thr Phe Met Leu
355 360 365
Ile Ser Phe Ile Gln Ser Phe Leu Ala Lys Tyr Pro Ser Ala Arg Pro
370 375 380
Leu Val Val Leu Pro Lys Gly Ile Leu Gly Thr Trp Lys Arg Glu Phe
385 390 395 400
Gln Arg Trp Gln Val Glu Asp Ile Pro Leu Tyr Asp Phe Tyr Ser Val
405 410 415
Lys Ala Asp Lys Arg Thr Glu Gln Leu Glu Val Leu Lys Ser Trp Glu
420 425 430
Ala Arg Met Ser Ile Leu Phe Leu Gly Tyr Lys Gln Phe Ser Arg Ile
435 440 445
Ile Cys Gly Asp Gly Asp Gly Asn Ile Ala Ala Ala Cys Arg Asp Arg
450 455 460
Leu Leu Met Val Pro Asn Leu Leu Ile Leu Asp Glu Gly His Thr Pro
465 470 475 480
Arg Asn Arg Glu Thr Asp Val Leu Ala Ser Leu Lys Arg Val Gln Thr
485 490 495
Pro Arg Lys Val Val Leu Ser Gly Thr Leu Phe Gln Asn His Val Ser
500 505 510
Glu Val Phe Asn Ile Leu Asp Leu Val Arg Pro Lys Phe Leu Lys Met
515 520 525
Glu Ser Ser Arg Pro Ile Ala Arg Arg Ile Met Ser Gln Val Ala Ile
530 535 540
Ser Gly Ile Arg Ser Leu Lys Gly Val His Asp Ser Ala Phe Thr Glu
545 550 555 560
Ser Val Glu Asp Thr Leu Leu Asn Asp Asp Asn Phe Thr Arg Lys Ser
565 570 575
His Val Ile Arg Ser Leu Arg Glu Leu Thr Lys Asp Val Leu His Tyr
580 585 590
Tyr Lys Gly Asp Ile Leu Asp Glu Leu Pro Gly Leu Val Asp Phe Ser
595 600 605
Val Phe Leu Lys Leu Ser Thr Lys Gln Lys Glu Ile Val His Lys Ile
610 615 620
Glu Ala Tyr Glu Lys Phe Lys Arg Ser Ala Val Gly Thr Ala Leu Tyr
625 630 635 640
Ile His Pro Cys Leu Ser Glu Ile Ser Glu Gly Asp Ala Ala Asp Arg
645 650 655
Ala Thr Asn Leu Thr Asp Ala Thr Val Asp Ser Leu Ile Glu Ser Ile
660 665 670
Ile Ile Lys Asp Gly Val Lys Ala Lys Phe Phe Phe Asn Ile Leu Ser
675 680 685
Leu Ala Asn Ser Ala Gly Glu Lys Leu Leu Ala Phe Ser Gln Tyr Ile
690 695 700
Leu Pro Met Lys Phe Leu Glu Arg Leu Leu Val Lys Arg Leu Gly Trp
705 710 715 720
His Val Gly Lys Glu Ile Phe Met Ile Ser Gly Asp Thr Ser Ala Asp
725 730 735
Asp Arg Glu Val Ala Met Asp Gln Phe Asn Asn Ser Ala Asp Ala Lys
740 745 750
Val Leu Phe Gly Ser Ile Lys Ala Cys Gly Glu Gly Ile Ser Leu Val
755 760 765
Gly Ala Ser Arg Val Ile Ile Leu Asp Val His Leu Asn Pro Ser Val
770 775 780
Thr Arg Gln Ala Ile Gly Arg Ala Phe Arg Pro Gly Gln Gln Lys Lys
785 790 795 800
Val Phe Val Tyr Arg Leu Val Ala Ala Asp Ser Pro Glu Val Lys Phe
805 810 815
His Glu Thr Ala Phe Lys Lys Glu Val Ile Pro Lys Leu Trp Phe Glu
820 825 830
Trp Ser Glu Leu Cys Thr Thr Glu Asp Phe Lys Leu Asn Gln Val Asp
835 840 845
Ile Asp Asp Ser Glu Asp Glu Leu Leu Glu Ala Asn Ala Ile Arg Gln
850 855 860
Asp Ile Lys Ala Leu Tyr Arg Arg
865 870
<210> 86
<211> 984
<212> PRT
<213> 二穗短柄草
<400> 86
Met Ala Ala Ala Gly Glu Asp Pro Gln Glu Pro His Ser Gly Asp Cys
1 5 10 15
Glu Arg Leu Pro Leu His His His Leu Leu Leu Ser His Leu Val Val
20 25 30
Val Ser Pro Ser Ala Pro Ala Val Gly Ser Asp Leu Glu Phe Gly Leu
35 40 45
Val Glu Ala Gly Lys Gly Met Tyr Tyr Arg Arg Lys Asn Arg Ala Ser
50 55 60
Asp Pro Ile Ser Gly Leu Ser Leu Pro Ala Gly Ser Gly Val His Ser
65 70 75 80
Trp Gly Cys Gly Ser Val Thr Lys Asp Met Glu Asp Ile Tyr Ala Arg
85 90 95
Asn Val Gln Leu Ile Asn Phe Leu Ser Thr Leu His Glu Pro Thr Arg
100 105 110
Ser Ser Val Pro Met Val Glu Thr Asn Val Lys Tyr Cys Ser Ile Lys
115 120 125
Gln Glu Thr Lys Val Thr Asp Cys Ser Val Lys Gln Thr Glu Pro Ile
130 135 140
Leu Ile Pro Asp Ser Asp Asp Glu Asp Gly Ser Thr Ala Glu Leu Ala
145 150 155 160
Pro Glu Lys Asn Lys Glu Leu Ile Pro Leu Gly Leu Ala Gly Thr Leu
165 170 175
Thr Ala His Val Thr Ser Lys Gly Lys Asp Gln Val Asn Glu Thr Arg
180 185 190
His Tyr Gly Tyr Gln Asn Ser Gln Ile Val Pro Tyr Gly Gln Ser Ala
195 200 205
Ala Leu Ile Asn His His Ser Leu Gln Thr Ser Trp Gln Pro Ser Ile
210 215 220
Gln Tyr Glu Ser Val Ile Leu Gln Thr Arg Thr Glu Glu Glu Arg Ile
225 230 235 240
Lys Tyr Leu Ala Ala Ala Ser His Ala Glu Lys Met Ala Glu Thr Gln
245 250 255
Val Phe Pro Asp Leu Pro Arg Glu Arg Lys Gln Arg Lys Leu Asp Pro
260 265 270
Asn Ser Gln Val Asp Gly Asp Ala Gly Thr Ala Pro Arg Lys Arg Lys
275 280 285
Arg Lys Thr Gly Pro Asp Pro Ala Ala Val Asp Leu Pro Ser Glu Thr
290 295 300
Tyr Asn Pro Val Glu Glu Glu Glu Pro Ala Glu Glu Asp Lys Pro Glu
305 310 315 320
Asn Lys Ser Asp Gly Leu Glu Asp Leu Trp Lys Asp Phe Ser Val Ala
325 330 335
Met Glu Ser Ser Lys Leu Asn Thr Phe Glu Glu Leu Pro Asp Glu Lys
340 345 350
Glu Leu Gly Glu Lys Asp Val Asp Asn Asp Cys Asn His Asp Ile Arg
355 360 365
Ile His Glu Asp Leu Gly His Val Cys Arg Val Cys Gly Leu Ile Val
370 375 380
Arg Arg Ala Asp Thr Ile Ile Asp Tyr Gln Trp Lys Lys Ala Ser Arg
385 390 395 400
Ser Arg Ser Tyr Phe Cys Gly Thr Arg Ser Lys Asp Ala Asp Glu Ile
405 410 415
Ile Ile Gly Asp Ile Arg Val Ser Asp Asp Leu Leu Ala Leu Asp Ile
420 425 430
Ala Ile His Pro Arg His Lys Lys Gln Ile Arg Ser His Gln Leu Glu
435 440 445
Gly Phe His Phe Leu Val Lys Asn Leu Val Ser Asp Lys Pro Gly Gly
450 455 460
Cys Ile Leu Ala His Ala Pro Gly Ser Gly Lys Thr Phe Met Val Ile
465 470 475 480
Ser Phe Ile Gln Ser Phe Leu Ala Lys Tyr Pro Ser Gly Arg Pro Leu
485 490 495
Val Ile Leu Pro Lys Gly Ile Leu Gly Thr Trp Lys Lys Glu Phe Gln
500 505 510
Gln Trp Gln Val Glu Asp Ile Pro Leu Tyr Asp Phe Tyr Ser Val Lys
515 520 525
Ala Glu Lys Arg Glu Asp Gln Leu Lys Ile Leu Asn Ser Trp Gln Ser
530 535 540
Lys Met Ser Ile Leu Phe Leu Gly Tyr Lys Gln Phe Ser Thr Ile Ile
545 550 555 560
Cys Gly Asp Gly Gly Gly Thr Val Ala Ala Ala Cys Arg Asp Met Leu
565 570 575
Leu Met Val Pro Asn Leu Leu Ile Leu Asp Glu Gly His Thr Pro Arg
580 585 590
Asn Thr Ala Thr Asn Val Leu Glu Ser Leu Ser Arg Val Gln Thr Pro
595 600 605
Arg Lys Val Val Leu Ser Gly Thr Leu Phe Gln Asn His Val Gly Glu
610 615 620
Val Phe Asn Ile Leu Asn Leu Val Arg Pro Lys Phe Leu Arg Met Glu
625 630 635 640
Ser Ser Arg Pro Ile Val Arg Arg Ile Met Ser Gln Val Ala Ile Ser
645 650 655
Gly Thr Arg Val Ser Lys Gly Val Pro Asp Asn Val Phe Thr Glu Ser
660 665 670
Val Glu Glu Thr Leu Leu His Asp Glu Asn Phe Thr Arg Lys Ala His
675 680 685
Ile Ile Arg Ser Leu Arg Glu Leu Thr Asn Asp Val Leu His Tyr Tyr
690 695 700
Lys Gly Asp Ile Leu Asp Glu Leu Pro Gly Leu Val Asp Phe Ser Val
705 710 715 720
Phe Leu Lys Leu Ser Pro Arg Gln Lys Glu Ile Val His Lys Leu Glu
725 730 735
Ala Tyr Glu Lys Phe Lys Arg Ser Ala Val Gly Thr Ala Leu Tyr Met
740 745 750
His Pro Cys Leu Ser Glu Met Ser Glu Gly Asp Ala Thr Asp Arg Ala
755 760 765
Asn Asn Leu Thr Asp Ala Ala Val Asp Ser Met Val Gln Ser Ile Asn
770 775 780
Val Arg Asp Gly Val Lys Ala Ser Phe Phe Ile Asn Ile Leu Arg Leu
785 790 795 800
Ala Ser Cys Ala Gly Glu Lys Leu Leu Ala Phe Ser Gln Tyr Ile Leu
805 810 815
Pro Met Lys Phe Leu Glu Arg Leu Leu Val Lys Thr Trp Gly Trp His
820 825 830
Val Gly Lys Glu Ile Phe Val Ile Ser Gly Asp Thr Ser Pro Glu Asp
835 840 845
Arg Glu Leu Ala Met Asp Gln Phe Asn Asn Ser Ala Asp Ala Lys Val
850 855 860
Leu Phe Gly Ser Ile Lys Ala Cys Gly Glu Gly Ile Ser Leu Val Gly
865 870 875 880
Ala Ser Arg Val Val Val Leu Asp Val His Leu Asn Pro Ser Val Thr
885 890 895
Arg Gln Ala Ile Gly Arg Ala Phe Arg Pro Gly Gln Gln Lys Lys Val
900 905 910
Phe Val Tyr Arg Leu Val Ala Ala Asp Ser Ala Glu Glu Ser Phe His
915 920 925
Glu Thr Ala Phe Lys Lys Glu Val Ile Pro Lys Leu Trp Phe Glu Trp
930 935 940
Ser Glu Gln His Cys Thr Thr Asp Asp Phe Lys Leu Asn Gln Thr Asp
945 950 955 960
Ile Asp Val Cys Gly Asp Glu Leu Leu Asp Asn Gln Ala Met Arg Gln
965 970 975
Asp Ile Lys Ala Leu Tyr Arg Arg
980
<210> 87
<211> 859
<212> PRT
<213> 毛果杨
<400> 87
Met Glu Thr Pro Leu Asn Ile Leu Pro Leu Lys Arg Ala Ala Pro Asn
1 5 10 15
Glu Ser Arg Phe Asn Gly Asn Lys Arg Met Lys Gln Met Lys Glu Lys
20 25 30
Val Thr Asp Tyr Ser Asp Pro Phe Ala Ile His Gly Leu Leu Asp Arg
35 40 45
Leu Asp Ser Gly Arg Tyr Gly Ser Val Thr Asp Asp Ile Arg Ser Leu
50 55 60
Phe Asn Arg Lys Ala Gln Met Ile His Thr Phe Leu Val Met Tyr Pro
65 70 75 80
Glu Leu Ala Asn Glu Ser Arg Gly Arg Gly Met Ser Phe Ser Glu Glu
85 90 95
Lys Cys Asn Val Ile Asp Leu Asp Asp Gly Asp Asp Asp Glu Gly Gly
100 105 110
Gly Gly Asn Val Ala Ala Gly Arg Met Pro Val Val Val Ile Asp Ser
115 120 125
Asp Asp Glu Val Glu Ser Asn Glu Asn Arg Met Ala Gly His Phe Gln
130 135 140
Gly Ile Val Leu Pro Lys Pro Glu Gly Gln Phe Leu Thr Asp Leu Met
145 150 155 160
Phe Ser Asp His Ala Glu Arg Arg Ile His Gly Glu Val Val Ser Leu
165 170 175
Thr Gly Glu Pro Asp Ile Thr Lys Asp Lys Gly Val Tyr Val Gly Val
180 185 190
Glu Glu Asp Glu Val Asp Thr Gly Ile Glu Asp Asp Gly Leu Gly Asp
195 200 205
Ile Trp Lys Glu Met Ser Phe Ala Leu Glu Ser Ser Lys Asp Val Val
210 215 220
Glu Asn Pro Gln Pro Asp Glu Asn Met Glu Glu Asp Glu Asp Tyr Cys
225 230 235 240
Asp His Ser Phe Val Leu Lys Asp Asp Ile Gly Tyr Val Cys Arg Ile
245 250 255
Cys Gly Val Ile Glu Lys Ala Ile Asp Thr Ile Ile Glu Ile Gln Phe
260 265 270
Asn Lys Val Lys Arg Asn Thr Arg Thr Tyr Met Ser Glu Ser Arg Asn
275 280 285
Ala Lys Asp Arg Asp Ser Asn Gly Met Val Gly Val Asp Leu Phe Glu
290 295 300
Glu Asp Leu Thr Leu Thr Asp Ile Pro Ala His Pro Arg His Met Lys
305 310 315 320
Gln Met Lys Pro His Gln Val Glu Gly Phe Asn Phe Leu Arg Asn Asn
325 330 335
Leu Val Ala Asp Asn Pro Gly Gly Cys Ile Leu Ala His Ala Pro Gly
340 345 350
Ser Gly Lys Thr Phe Met Ile Ile Ser Phe Met Gln Ser Phe Leu Ala
355 360 365
Lys Tyr Pro His Ala Lys Pro Leu Val Val Leu Pro Lys Gly Ile Leu
370 375 380
Ser Thr Trp Lys Lys Glu Phe Gln Ile Trp Gln Ile Glu Asp Ile Pro
385 390 395 400
Leu Tyr Asp Phe Tyr Ser Val Lys Ala Asp Ser Arg His Gln Gln Leu
405 410 415
Glu Val Leu Lys Gln Trp Leu Glu His Lys Ser Ile Leu Phe Leu Gly
420 425 430
Tyr Lys Gln Phe Ser Ser Ile Val Cys Asp Asp Gly Lys Asn Gln Val
435 440 445
Ser Val Thr Cys Gln Glu Ile Leu Leu Arg Arg Pro Ser Ile Leu Ile
450 455 460
Leu Asp Glu Gly His Thr Pro Arg Asn Glu Asn Thr Asp Val Leu Gln
465 470 475 480
Ser Leu Ala Lys Val Gln Thr Pro Arg Lys Val Val Leu Ser Gly Thr
485 490 495
Leu Tyr Gln Asn His Val Lys Glu Val Phe Asn Val Leu Asn Leu Val
500 505 510
Arg Pro Lys Phe Leu Arg Met Asp Thr Ser Arg Gly Ile Val Lys Arg
515 520 525
Ile Leu Ser Lys Val Asn Ile Pro Gly Ala Arg Lys Gln Phe Lys Ala
530 535 540
Gly Ala Asp Ala Ala Phe Tyr Asp Leu Val Glu His Thr Met Gln Lys
545 550 555 560
Asp Gln Asp Phe Lys Arg Lys Val Thr Val Ile Arg Asp Leu Arg Glu
565 570 575
Met Thr Ser Lys Val Leu His Tyr Tyr Lys Gly Asp Phe Leu Asp Glu
580 585 590
Leu Pro Gly Leu Val Asp Phe Thr Val Val Leu Asn Leu Ser Ser Lys
595 600 605
Gln Lys His Glu Val Gln Lys Leu Lys Lys Phe Ala Gly Lys Phe Lys
610 615 620
Arg Ser Ser Val Gly Ser Ala Val Tyr Leu His Pro Lys Leu His Ser
625 630 635 640
Phe Ser Glu Asn Ser Ala Val Thr Asp Asp Met Met Asp Asn Leu Leu
645 650 655
Glu Thr Leu Asp Val Arg Asp Gly Ala Lys Ala Lys Phe Phe Leu Asn
660 665 670
Ile Leu Ser Leu Cys Lys Ser Ala Gly Glu Lys Leu Leu Val Phe Ser
675 680 685
Gln Tyr Leu Thr Pro Leu Lys Phe Leu Glu Arg Leu Val Met Lys Val
690 695 700
Lys Gly Trp Ile Leu Gly Lys Asp Ile Phe Val Ile Ser Gly Glu Ser
705 710 715 720
Ser Ser Asp His Arg Glu Trp Ser Met Asp Arg Phe Asn Asn Ser Ile
725 730 735
Asp Ala Lys Val Phe Phe Gly Ser Ile Lys Ala Cys Gly Glu Gly Ile
740 745 750
Ser Leu Val Gly Ala Ser Arg Ile Ile Ile Leu Asp Val His Leu Asn
755 760 765
Pro Ser Val Thr Arg Gln Ala Ile Gly Arg Ala Phe Arg Pro Gly Gln
770 775 780
Thr Lys Lys Val Tyr Ala Tyr Arg Leu Val Ala Ala Asp Ser Pro Glu
785 790 795 800
Glu Glu Asp His Thr Thr Cys Phe Arg Lys Glu Ala Ile Ala Lys Met
805 810 815
Trp Phe Glu Trp Asn Glu Tyr Cys Gly Tyr Gln Asp Phe Glu Val Glu
820 825 830
Thr Val Glu Leu Asp Asp Ser Gly Asp Cys Phe Leu Glu Ser Leu Leu
835 840 845
Val Arg Asp Asp Val Arg Leu Leu Tyr Lys Arg
850 855
<210> 88
<211> 903
<212> PRT
<213> 葡萄
<400> 88
Met His Ser Glu Pro Lys Gln Lys Arg Gln Lys Ala Gly Pro Asn Val
1 5 10 15
Val Asp Tyr Ser Asp Pro Phe Ala Ile Pro Asn Leu Leu Glu Gly Leu
20 25 30
Asp Ala Gly Arg Phe Gly Ser Val Thr Lys Glu Ile Glu Ala Leu Cys
35 40 45
Ala Arg Arg Met Gln Met Leu Gln Pro Tyr Tyr Val Met Tyr Pro Ser
50 55 60
Leu Ser Tyr Met Cys Thr Asp Leu Gly Lys Lys Gln Gly Lys Lys Ala
65 70 75 80
Ser Lys Leu Val Asn Arg Glu Ala Ser His Leu Ala His Glu Asp Val
85 90 95
Ile Asp Leu Glu Asp Asp His Val Val Asp Asp Ala Leu Thr Ala Thr
100 105 110
Ala Val Glu Asp Ala Thr Leu Pro Val Val Ile Ile Asp Ser Asp Asp
115 120 125
Glu Asp Cys Gly Asp Gln Lys Val Ser His Pro Pro Gln Glu Thr Ala
130 135 140
Trp Pro Ser Phe Ser Tyr Gln Glu Val Val Leu Arg Lys Pro Ser Val
145 150 155 160
Gly Leu Leu Ala Asn Asn Pro Val Val Arg Asp Tyr Val Gly Ser Ile
165 170 175
Ala Pro Lys Val Glu Glu Gly Ser Leu Met Gly Ala Thr Glu Ile Arg
180 185 190
Lys Asp Lys Asp Val Tyr Ile Gly Val Gly Glu Lys Ser Leu Val Ala
195 200 205
Asn Leu Glu Met Lys Lys Val Gln Gly Glu Tyr Val Gly Val Glu Asp
210 215 220
Asp Met Glu Thr Asn Glu Gly Asn Leu Arg Ala Lys Thr Glu Asp Asp
225 230 235 240
Gly Leu Ala Asp Met Trp Gln Glu Phe Asp Leu Ala Leu Gln Ser Ser
245 250 255
Lys Asp Val Ala Val Asp Pro Gly Glu Asp Glu Lys Glu Ser Lys Glu
260 265 270
Glu Cys Glu His Ser Phe Val Leu Lys Asp Asp Ile Gly Ser Val Cys
275 280 285
Arg Ile Cys Gly Val Val Asn Lys Ser Ile Glu Thr Ile Ile Glu Tyr
290 295 300
Gln Tyr Thr Lys Val Lys Arg Ser Arg Thr Tyr Met Tyr Glu Pro Arg
305 310 315 320
Asn Thr Lys Asp Arg Glu Pro Thr Asp Asp Pro Ser Asp Gly Leu Gly
325 330 335
Phe Ser Glu His Asn Leu Thr Val Thr Glu Ile His Ala His Pro Arg
340 345 350
His Ser Met Gln Met Lys Pro His Gln Val Glu Gly Phe Asn Phe Leu
355 360 365
Val Ser Asn Leu Val Ala Glu Asn Pro Gly Gly Cys Ile Leu Ala His
370 375 380
Ala Pro Gly Ser Gly Lys Thr Phe Met Ile Ile Ser Phe Met Gln Ser
385 390 395 400
Phe Leu Ala Lys Tyr Pro Gln Ala Arg Pro Leu Val Val Leu Pro Lys
405 410 415
Gly Ile Leu Ala Thr Trp Lys Lys Glu Phe Leu Thr Trp Gln Val Glu
420 425 430
Asp Ile Pro Leu Tyr Asp Phe Tyr Ser Val Lys Ala Asp Ser Arg Pro
435 440 445
Gln Gln Leu Glu Val Leu Lys Gln Trp Val Ala Glu Lys Ser Ile Leu
450 455 460
Phe Leu Gly Tyr Lys Gln Phe Ser Ser Ile Val Cys Gly Asp Gly Ala
465 470 475 480
Ser Lys Ala Thr Ile Ala Cys Gln Glu Ile Leu Leu Lys Ala Pro Gln
485 490 495
Ile Leu Ile Leu Asp Glu Gly His Thr Pro Arg Asn Glu Asn Thr Asp
500 505 510
Val Leu Tyr Ser Leu Ala Lys Val Gln Thr Pro Arg Lys Val Val Leu
515 520 525
Ser Gly Thr Leu Tyr Gln Asn His Val Lys Glu Val Phe Asn Ile Leu
530 535 540
Asn Leu Val Arg Pro Lys Phe Leu Lys Leu Glu Ser Ser Arg Ala Val
545 550 555 560
Val Lys Arg Ile Met Ser Lys Val Asp Ile Met Gly Val Arg Lys Gln
565 570 575
Leu Lys Ser Asn Ala Ala Asp Ala Phe Tyr Asp Leu Val Glu Asn Thr
580 585 590
Leu Gln Lys Asp Asp Asn Phe Arg Arg Lys Ile Thr Val Ile Gln Asp
595 600 605
Leu Arg Glu Met Thr Ser Lys Val Leu His Tyr Tyr Lys Gly Asp Phe
610 615 620
Leu Asp Glu Leu Pro Gly Leu Val Asp Phe Thr Val Leu Leu Asn Leu
625 630 635 640
Ser Ala Arg Gln Lys Lys Glu Val Gly Asn Leu Asn Lys Phe Glu Arg
645 650 655
Lys Phe Lys Lys Asn Ser Val Gly Ser Ala Val Tyr Leu His Pro Gln
660 665 670
Leu Lys Tyr Phe Ala Glu Lys Leu Ala Ala Asn Glu Ser Lys Thr Asp
675 680 685
Glu Met Thr Cys Gln Lys Lys Met Asp Glu Ile Leu Glu Gln Leu Asp
690 695 700
Val Arg Asp Gly Val Lys Ala Lys Phe Phe Leu Asn Val Leu Ala Leu
705 710 715 720
Cys Gln Ser Ser Gly Glu Lys Leu Leu Val Phe Ser Gln Tyr Leu Leu
725 730 735
Pro Leu Arg Phe Leu Glu Lys Leu Thr Met Lys Val Lys Gly Trp Ser
740 745 750
Pro Gly Lys Glu Ile Phe Ala Ile Ser Gly Glu Ser Ser Ser Glu Gln
755 760 765
Arg Glu Trp Ser Met Glu Arg Phe Asn Thr Ser Pro Asp Ala Arg Val
770 775 780
Phe Phe Gly Ser Ile Lys Ala Cys Gly Glu Gly Ile Ser Leu Val Gly
785 790 795 800
Ala Ser Arg Val Leu Ile Leu Asp Val His Leu Asn Pro Ser Val Thr
805 810 815
Arg Gln Ala Ile Gly Arg Ala Phe Arg Pro Gly Gln Lys Lys Lys Val
820 825 830
His Val Tyr Lys Leu Val Ala Ala Asp Ser Pro Glu Glu Glu Asp His
835 840 845
Asn Ser Cys Phe Lys Lys Glu Leu Ile Ser Lys Met Trp Phe Glu Trp
850 855 860
Asn Glu Tyr Cys Gly His His Glu Phe Glu Ala Glu Thr Val Asp Val
865 870 875 880
Ser Asp Ser Gly Asp Leu Phe Leu Glu Ser Pro Leu Leu Arg Glu Asp
885 890 895
Ile Thr Val Leu Tyr Arg Arg
900
<210> 89
<211> 903
<212> PRT
<213> 黄瓜
<400> 89
Met Asp Ala Thr Ile Asp Phe Ser His Asn Ser Tyr Ala Thr Thr Asn
1 5 10 15
Gly Leu Tyr Tyr Gly Lys His Lys Arg Leu Lys Leu Ser Ser Asp Gly
20 25 30
Lys Asp Leu Ser Ser Thr Ala Thr Phe Ser Ala Lys Lys Ser Asn Thr
35 40 45
Leu Arg Gln Asn Lys Met Asn Asn Ser Ala Lys Val Ile Asp Tyr Ser
50 55 60
Asp Pro Phe Ala Thr Asn Asn Leu Ile Asp Gly Leu Asp Cys Gly His
65 70 75 80
Phe Gly Ser Val Thr Lys Glu Ile Gly Ala Leu Val Ser Arg Lys Met
85 90 95
Gln Val Leu Ser Pro Tyr Ile Ala Lys Tyr Pro Ala Leu Ser Ser Met
100 105 110
Leu Phe Asp Leu Gly Arg Ser Arg Glu Cys Lys Glu Ala Met Asn Asn
115 120 125
Gln Ala Ser Gln Leu Val His Asn Leu Ile Asp Leu Glu Asp Asp Ser
130 135 140
Ala Ile Asp Val Arg Ser Asn Asn Val Glu Lys Ser Arg Leu Pro Ile
145 150 155 160
Leu Ile Ile Asp Ser Asp Glu Glu Asp Ser Lys Glu Gln Arg Val Ile
165 170 175
His Pro Phe Gln Glu Val Val Leu Pro Arg Pro Pro Gly Gln Ser Leu
180 185 190
Phe Lys Asp Ile Ala Ile Val Asp His Arg Thr Ser Arg Asp Arg Arg
195 200 205
Ala Ser Asn Gly Glu Glu Ala Thr Pro Ile Gly Glu Ser Gly Thr Ile
210 215 220
Asn Lys Asp Lys Gly Val Tyr Ile Gly Val Glu Glu Asp Glu Asp Glu
225 230 235 240
Val Ser Gly Gln Ala Asn Ser Glu Asp Asp Gly Leu Gly Asp Ile Trp
245 250 255
Asn Asp Met Gln Met Ala Leu Glu Cys Ser Lys Asp Leu Asp Ala Ala
260 265 270
Val Asp Ser Ser Ser Asn Gln Pro Thr Thr Glu Asp Val Asp Cys Glu
275 280 285
His Ser Phe Leu Leu Lys Asp Asp Leu Gly Tyr Val Cys Arg Ile Cys
290 295 300
Gly Val Ile Asp Arg Gly Ile Glu Thr Ile Phe Glu Phe Gln Tyr Asn
305 310 315 320
Lys Gly Lys Lys Ser Thr Arg Thr Tyr Ile Ser Glu Ser Arg Asn Lys
325 330 335
Asp Ser Gly Asn Ile Val Gly Val Lys Ile Ser Glu Asp Asp Leu Thr
340 345 350
Val Thr Glu Ile Ser Ala His Pro Arg His Met Lys Gln Met Lys Pro
355 360 365
His Gln Ile Glu Gly Phe Asn Phe Leu Ile Ser Asn Leu Val Ser Asp
370 375 380
Asn Pro Gly Gly Cys Ile Leu Ala His Ala Pro Gly Ser Gly Lys Thr
385 390 395 400
Phe Met Ile Ile Ser Phe Met Gln Ser Phe Leu Ala Lys Tyr Pro Gln
405 410 415
Ala Arg Pro Leu Val Val Leu Pro Lys Gly Ile Leu Ala Thr Trp Lys
420 425 430
Lys Glu Phe Gln Ile Trp Gln Val Glu Asp Ile Pro Leu Tyr Asp Phe
435 440 445
Tyr Ser Val Lys Ala Asp Asn Arg Ala Gln Gln Leu Thr Val Leu Asn
450 455 460
Gln Trp Val Glu His Lys Ser Ile Leu Phe Leu Gly Tyr Lys Gln Phe
465 470 475 480
Ser Thr Ile Val Cys Asp Val Glu Thr Ser Ala Ala Ser Thr Ala Cys
485 490 495
Gln Asn Ile Leu Leu Gln Val Pro Thr Ile Leu Ile Leu Asp Glu Gly
500 505 510
His Thr Pro Arg Asn Glu Asn Thr Asp Thr Leu Gln Thr Leu Ala Lys
515 520 525
Val Arg Thr Pro Arg Lys Val Val Leu Ser Gly Thr Leu Tyr Gln Asn
530 535 540
His Val Lys Glu Val Phe Asn Ile Val Asn Leu Val Arg Pro Lys Phe
545 550 555 560
Met Arg Ser Glu Thr Ser Arg Pro Ile Ile Lys Arg Ile Met Ser Arg
565 570 575
Val Asp Ile Pro Gly Ala Arg Lys Gln Phe Lys Ala Gly Val Asp Ala
580 585 590
Ala Phe Tyr Asp Leu Val Glu His Thr Leu Gln Lys Asp Thr Asp Phe
595 600 605
Arg Arg Lys Val Ser Val Ile His Asp Leu Arg Glu Met Thr Ser Lys
610 615 620
Ile Leu His Tyr Tyr Lys Gly Asp Phe Leu Asp Glu Leu Pro Gly Leu
625 630 635 640
Val Asp Phe Thr Val Val Leu Asn Leu Thr Ser Lys Gln Lys His Glu
645 650 655
Gly Glu Lys Val Lys Lys Phe Asn Arg Lys Phe Lys Ile Ser Ser Ala
660 665 670
Gly Ser Ala Val Tyr Leu His Pro Lys Leu Asn Val Phe Ser Val Asn
675 680 685
Ala Ala Val Thr Asp Asp Lys Ile Asp Glu Val Ile Asp Lys Met Asp
690 695 700
Val Lys Asp Gly Val Lys Thr Lys Phe Phe Leu Asn Leu Leu Asn Leu
705 710 715 720
Cys Ala Thr Thr Gly Glu Lys Leu Leu Val Phe Ser Gln Tyr Leu Leu
725 730 735
Pro Leu Lys Phe Met Glu Arg Leu Val Val Gln Lys Lys Gly Trp Ser
740 745 750
Pro Gly Arg Glu Thr Phe Met Ile Ser Gly Glu Thr Thr Pro Glu Gln
755 760 765
Arg Glu Trp Ser Met Glu Arg Phe Asn Asn Ser Pro Asp Ala Arg Val
770 775 780
Phe Phe Gly Ser Ile Lys Ala Cys Gly Glu Gly Ile Ser Leu Val Gly
785 790 795 800
Ala Ser Arg Ile Ile Ile Leu Asp Val His Leu Asn Pro Ser Val Thr
805 810 815
Arg Gln Ala Ile Gly Arg Ala Phe Arg Pro Gly Gln Thr Lys Lys Val
820 825 830
Phe Ala Tyr Arg Leu Val Ala Gly Asp Ser Pro Glu Glu Gly Asp His
835 840 845
Ser Thr Cys Phe Lys Lys Glu Leu Ile Ala Lys Met Trp Phe Glu Trp
850 855 860
Asn Glu Tyr Cys Gly Tyr His Asp Phe Glu Val Glu Thr Val Asp Val
865 870 875 880
Lys Gln Cys Gly Asp Asn Phe Leu Glu Thr Pro Leu Leu Gly Gln Asp
885 890 895
Val Lys Val Leu Tyr Arg Arg
900
<210> 90
<211> 815
<212> PRT
<213> 琴叶拟南芥
<400> 90
Met Phe Val Phe Ala Lys Lys Leu Lys Ser Leu Pro Ala Ser Lys His
1 5 10 15
Glu Arg Ser Glu Ala Asn Gln Lys Ser Ser Ser Leu Arg Cys His Ile
20 25 30
Gln Phe Leu Phe Ile Phe Leu Lys Phe Ser Ser Lys Val Pro Thr Phe
35 40 45
Asn Ser Leu Met Glu Ile Leu His Pro Asp Gln Ala Thr Ser Asn Gly
50 55 60
Phe Tyr Lys Asn Val His Lys Arg Lys Gln Asn Gln Val Asp Asp Gly
65 70 75 80
Leu Cys Ser Asp Ser Glu Ala Lys Arg Leu Lys Ser Ser Ser Lys Val
85 90 95
Ile Asp Tyr Ser Asn Pro Phe Ala Ile Ser Asn Met Leu Glu Ala Leu
100 105 110
Asp Gly Gly Lys Phe Gly Ser Val Thr Lys Glu Leu Gln Glu Ile Ala
115 120 125
Asp Met Arg Met Asp Leu Ile Lys Arg Cys Ile Trp Leu Tyr Pro Ser
130 135 140
Leu Ala Tyr Thr Glu Phe Glu Asp Glu Lys Thr Met Leu Ser Leu Asp
145 150 155 160
Asn Gln Gln Val Val Glu Gly Gly Val Ile Asn Leu Asp Asp Asp Asp
165 170 175
Asp Asp Asp Asp Asp Val Ser Asn Lys Ala Ile Cys Val Val Pro Ser
180 185 190
Ser Glu Ile Val Val Leu Asp Ser Asp Asp Glu Asp Asn Glu Arg Gln
195 200 205
Arg Ser Thr Tyr Gln Phe Gln Ser Thr Leu Val Gln Leu Gln Lys Asn
210 215 220
Gln Gly Asp Val Thr Pro Val Thr Pro Gln Phe Thr Phe Glu Glu Val
225 230 235 240
Asp Leu Gly Arg Ser Lys Glu Met Met Pro Ser Val Ile Lys Ala Ile
245 250 255
Val Glu Gly Gln Thr Ser Arg Asp Lys Val Ile Ala Ile Glu Asn Gly
260 265 270
Val Ala Asn Glu Lys Gly Val Tyr Val Gly Val Glu Asp Asp Asp Ile
275 280 285
Asp Asn Glu Ser Glu Ala Ala Asp Glu Asp Leu Gly Asn Ile Trp Asn
290 295 300
Glu Met Ala Met Ser Ile Glu Cys Ser Lys Asp Ile Ala Trp Glu Thr
305 310 315 320
Ser His Lys Gln Lys Ala Asp Val Val Glu Asp Cys Glu His Ser Phe
325 330 335
Ile Leu Lys Asp Asp Met Gly Tyr Val Cys Arg Val Cys Gly Val Ile
340 345 350
Glu Lys Ser Ile Leu Glu Ile Ile Asp Val Gln Phe Thr Lys Ala Lys
355 360 365
Arg Asn Thr Arg Thr Tyr Ala Ser Glu Thr Arg Asn Lys Arg Phe Gly
370 375 380
Glu Ser Asp Ala Glu Leu Lys Phe Ser Glu Glu Gly Leu Met Ile Gly
385 390 395 400
Gly Leu Ala Ala His Pro Thr His Ala Asp Lys Met Lys Pro His Gln
405 410 415
Ile Glu Gly Phe Gln Phe Leu Cys Ser Asn Leu Val Ala Asp Asp Pro
420 425 430
Gly Gly Cys Ile Met Ala His Ala Pro Gly Ser Gly Lys Thr Phe Met
435 440 445
Ile Ile Ser Phe Met Gln Ser Phe Leu Ala Lys Tyr Pro Gln Ala Lys
450 455 460
Pro Leu Val Val Leu Pro Lys Gly Ile Leu Ser Thr Trp Lys Lys Glu
465 470 475 480
Phe Leu Arg Trp Gln Val Glu Asp Ile Pro Leu Leu Asp Phe Tyr Ser
485 490 495
Ala Arg Ala Glu Asn Arg Ala Gln Gln Leu Ala Ile Leu Arg Lys Trp
500 505 510
Met Glu Lys Lys Ser Ile Leu Phe Leu Gly Tyr Lys Gln Phe Ser Thr
515 520 525
Ile Val Cys Asp Asp Thr Ser Thr Asp Ser Leu Ser Cys Gln Glu Ile
530 535 540
Leu Leu Lys Val Pro Ser Ile Leu Ile Leu Asp Glu Gly His Thr Pro
545 550 555 560
Arg Asn Glu Asp Thr Asn Val Leu Gln Ser Leu Ala Gln Val Gln Thr
565 570 575
Pro Arg Lys Val Val Leu Ser Gly Thr Leu Tyr Gln Asn His Val Lys
580 585 590
Glu Val Phe Asn Ile Leu Asn Leu Val Arg Pro Lys Phe Leu Lys Leu
595 600 605
Asp Thr Ser Lys Ser Ile Val Lys Arg Ile Leu Ser Tyr Ala Pro Ile
610 615 620
Ser Asp Val Arg Ser His Met Gly Asn Asn Ser Asp Val Ser Ser Ala
625 630 635 640
Phe Asn Glu Ile Val Glu Tyr Thr Leu Gln Lys Ser Glu Asp Phe Thr
645 650 655
Met Lys Ile Asn Val Ile Gln Asp Leu Arg Glu Met Thr Lys Lys Val
660 665 670
Leu His Tyr Tyr Lys Gly Asp Phe Leu Asp Glu Leu Pro Gly Leu Ala
675 680 685
Asp Phe Thr Val Val Leu Asn Leu Ser Pro Arg Gln Leu Asn Glu Val
690 695 700
Lys Lys Leu Arg Arg Glu Lys Arg Lys Phe Lys Val Ser Ala Val Gly
705 710 715 720
Ser Ala Ile Tyr Leu His Arg Glu Ala Glu Thr Ile Gly Arg Ala Phe
725 730 735
Arg Pro Gly Gln Thr Lys Lys Val His Ala Tyr Arg Leu Ile Ala Gly
740 745 750
Ser Ser Pro Glu Glu Glu Asp His Asn Thr Cys Phe Lys Lys Glu Val
755 760 765
Ile Ser Lys Met Trp Phe Glu Trp Asn Glu Tyr Cys Gly Tyr Arg Asn
770 775 780
Phe Glu Val Glu Thr Ile Asp Val Asp Asp Ala Asp Asp Met Phe Leu
785 790 795 800
Glu Ser Pro Thr Leu Arg Glu Asp Ile Arg Val Leu Tyr Lys Arg
805 810 815
<210> 91
<211> 163
<212> PRT
<213> 拟南芥
<400> 91
Met Gln Ser Ser Met Thr Met Glu Leu Arg Pro Ser Gly Asp Ser Gly
1 5 10 15
Ser Ser Asp Val Asp Ala Glu Ile Ser Asp Gly Phe Ser Pro Leu Asp
20 25 30
Thr Ser His Arg Asp Val Ala Asp Glu Gly Ser Leu Leu Arg Arg Ala
35 40 45
Glu Met Tyr Gln Asp Tyr Met Lys Gln Val Pro Ile Pro Thr Asn Arg
50 55 60
Gly Ser Leu Ile Pro Phe Thr Ser Trp Val Gly Leu Ser Ile Ser Met
65 70 75 80
Lys Gln Leu Tyr Gly Gln Pro Leu His Tyr Leu Thr Asn Val Leu Leu
85 90 95
Gln Arg Trp Asp Gln Ser Arg Phe Gly Thr Asp Ser Glu Glu Gln Arg
100 105 110
Leu Asp Ser Ile Ile His Pro Thr Lys Ala Glu Ala Thr Ile Trp Leu
115 120 125
Val Glu Glu Ile His Arg Leu Thr Pro Ser His Leu His Met Ala Leu
130 135 140
Leu Trp Arg Ser Asp Pro Met Tyr His Ser Phe Ile Asp Pro Ile Phe
145 150 155 160
Pro Glu Lys
<210> 92
<211> 175
<212> PRT
<213> 蓖麻
<400> 92
Met Lys Arg Thr Met Pro Trp Asn Glu Gln Ile Asp Val Ile Ser Ser
1 5 10 15
Asp Glu Ser Thr Ser Ser Asp Gly Asp Met Glu Thr Asp Val Val Leu
20 25 30
Asp Gly His Lys Pro Ser Pro Arg Ile Thr Ile Ser Gln Ser Leu Lys
35 40 45
Glu Ile Thr Ser Glu Asp Val Leu Ile Arg Arg Ala Glu Met Tyr Gln
50 55 60
Asp Tyr Met Arg Gln Leu Pro Ile Pro Val His His Gly Ser Val Ile
65 70 75 80
Pro Phe Ser Ser Trp Val Gly Leu Gly Lys Ser Ile Lys Gln Leu Tyr
85 90 95
Gly Gln Pro Leu His Tyr Leu Thr Asn Ile Leu Leu Lys Gln Trp Asp
100 105 110
Gln Leu Arg Phe Asp Asn Gly Asp Glu Gln Lys Ser Leu Asp Ile Val
115 120 125
Ile His Pro Cys Lys Ala Glu Ala Ser Val Trp Leu Val Glu Glu Ile
130 135 140
His Arg Cys Thr Ser Ser Pro His Tyr Val Ala Lys Leu Trp Leu Leu
145 150 155 160
Asp Pro Met His His Ala Phe Val Asp Ser Ile Phe Pro Pro Leu
165 170 175
<210> 93
<211> 165
<212> PRT
<213> 大豆
<400> 93
Met Lys Arg Ser Phe Pro Trp Glu Asn Gln Ile Asp Val Ser Ser Ser
1 5 10 15
Ser Ser Pro Asn Ser Leu Arg Pro Asn Pro Leu Thr Ile Pro Asn Ser
20 25 30
Pro Ile Asp Thr Lys Ser Gln Asp Ala Leu Ile Arg Arg Ala Glu Met
35 40 45
Tyr Gln Asp Tyr Met Lys Gln Ile Pro Ile Pro Asn His Arg Gly Thr
50 55 60
Met Ile Pro Phe Thr Ser Trp Met Gly Leu Gly Arg Ser Met Lys Gln
65 70 75 80
Ile Tyr Gly Gln Pro Leu His Tyr Leu Thr Asn Ile Leu Leu Lys Gln
85 90 95
Trp Asp Gln Leu Arg Ile Gly Ser Glu Asp Glu Tyr Lys Pro Leu Asp
100 105 110
Asn Ile Val His Pro His Lys Ala Glu Ala Thr Ile Trp Leu Ile Glu
115 120 125
Glu Ile His Arg Gln Thr Ser Ser His Phe His Leu Ala Ser Leu Trp
130 135 140
Lys Val Asp Pro Met Tyr Asn Gly Phe Val Asp Ser Ile Phe Pro Thr
145 150 155 160
Leu Glu His Thr Ser
165
<210> 94
<211> 218
<212> PRT
<213> 玉蜀黍
<400> 94
Met Lys Arg Ala Ala Pro Ser Gln Glu Pro Leu Asp Val Ser Ser Gly
1 5 10 15
Asp Ser Ser Gly Ser Asp Ser Asp Gln Leu Gly Gly Lys Asp Lys Gly
20 25 30
Gly Ser Ala Ser Lys Ala Ala Ala Gly Ala Glu Asp Val Lys Arg Val
35 40 45
Val Leu His Lys Glu Ser Gln Asp Val Ser Ser Asp Asp Asp Leu Ser
50 55 60
Ser Asp Ser Asp Asp Asp Ala Gly Lys Gly Asn Ala Trp Asn Ala Phe
65 70 75 80
Gly Leu Pro Asn Ser Ser Lys Ala Ala Ser Thr Ala Glu Gly Ala Leu
85 90 95
Ile Arg Arg Ala Glu Met Tyr Gln Gln Tyr Met Lys Arg Ile Pro Val
100 105 110
Pro Ala Tyr Arg Asp Ser Val Ile Pro Phe Thr Ser Trp Leu Gly Leu
115 120 125
Ala Gly Ser Leu Lys Gln Leu Tyr Glu Gln Pro Leu His Tyr Leu Thr
130 135 140
Asn Val Leu Leu Lys Lys Trp Asp Gln Gln Arg Ile Gly Ser Asp Asp
145 150 155 160
Glu His Arg Arg Leu Asp Ala Ile Ile His Pro Ala Arg Ala Glu Thr
165 170 175
Leu Val Trp Ala Thr Glu Glu Val His Arg Leu Thr Thr Ser Gly Gln
180 185 190
His Leu Ala Ser Leu Trp Ala Ser Asp Pro Met Tyr His Ala His Val
195 200 205
Asp Pro Val Phe Ala Ser Val Lys Leu Glu
210 215
<210> 95
<211> 179
<212> PRT
<213> 蒺藜苜蓿
<400> 95
Met Lys Arg Pro Phe Pro Trp Asp Glu Asp Glu Ile Asp Val Ser Asp
1 5 10 15
Ser Ser Ser Ser Asp Asp Ser Pro Asn Glu Thr Pro Asn Val Ala Thr
20 25 30
Val Leu Pro Ser Ile His Ile His Ile Pro Leu Pro Asn Pro Ser Ser
35 40 45
Asp Val Asn Ser Leu Asp Ala Leu Thr Arg Arg Ala Ala Met Tyr Gln
50 55 60
Asp Tyr Met Lys His Ile Pro Ile Pro Ser Ser Arg Gly Ser Leu Ile
65 70 75 80
Pro Phe Thr Ser Trp Met Gly Leu Gly Arg Ser Ile Lys Gln Leu Tyr
85 90 95
Gly Gln Pro Leu His Tyr Leu Thr Asn Ile Leu Leu Lys Gln Trp Asp
100 105 110
Gln Leu Arg Ile Gly Ser Glu Asp Glu Tyr Thr Pro Leu Asp Asp Ile
115 120 125
Ile His Pro Arg Lys Ala Glu Ser Thr Ile Trp Leu Met Glu Glu Ile
130 135 140
His Arg Gln Thr Ser Ser His Phe His Ile Ala Asp Leu Trp Lys Lys
145 150 155 160
Asp Pro Met Tyr His Gly Phe Ile Asp Ser Ile Phe Pro Lys Leu Glu
165 170 175
Asp Thr Ser
<210> 96
<211> 173
<212> PRT
<213> 稻
<400> 96
Met Lys Arg Ala Ala Pro Trp Glu Glu Pro Leu Glu Val Ser Ser Asp
1 5 10 15
Asp Ser Leu Ser Ser Asp Ser Asp Asp Glu Ala Gly Lys Gly Lys Gly
20 25 30
Asp Asn Ala Phe Gly Leu Pro Asn Ser Thr Lys Ala Ala Ala Pro Asp
35 40 45
Gly Ala Leu Ile Arg Lys Ala Glu Met Tyr Gln Glu Tyr Met Lys His
50 55 60
Ile Pro Val Pro Ala His Arg Gly Ser Val Ile Pro Cys Thr Thr Trp
65 70 75 80
Leu Gly Leu Gly Arg Ser Val Lys Gln Leu Tyr Lys Gln Pro Leu His
85 90 95
Tyr Leu Thr Asn Val Leu Leu Lys Gln Trp Asp Gln Gln Arg Val Gly
100 105 110
Ser Asp Asp Glu His Arg Pro Leu Asp Ala Ile Ile His Pro Val Lys
115 120 125
Ala Glu Ala Leu Ile Trp Ile Thr Glu Glu Val His Arg Leu Thr Thr
130 135 140
Ser Ser Gln His Leu Ala Ser Leu Trp Ala Ser Ala Pro Met Tyr His
145 150 155 160
Ala Tyr Ile Asp Pro Val Phe Pro Pro Ile Lys Leu Gln
165 170
<210> 97
<211> 175
<212> PRT
<213> 毛果杨
<400> 97
Met Lys Arg Thr Ile Pro Trp Thr Asp Pro Val Asp Val Ile Ser Ser
1 5 10 15
Asp Glu Ser Ser Ser Ser Asp Ser Asp Ile Glu Leu Asn Asp Gly Leu
20 25 30
Asp Gly Gln Arg Leu Ser Asn Tyr Val Thr Ile Asp Gln Pro Ile Lys
35 40 45
Glu Thr Thr Ser Glu Gly Val Leu Ile Arg Arg Ala Glu Met Tyr Gln
50 55 60
Asp Tyr Met Arg His Ile Pro Ile Pro Val Gln Arg Gly Ser Ala Ile
65 70 75 80
Pro Phe Ser Ser Trp Ala Gly Leu Gly Lys Ser Ile Lys Gln Leu Tyr
85 90 95
Gln Gln Pro Leu His Tyr Leu Thr Asn Ile Leu Leu Asn Lys Trp Asp
100 105 110
Gln Gln Arg Ile Gly Ser Glu Asp Glu His Thr Pro Leu Asp Ile Met
115 120 125
Ile His Pro Cys Lys Ala Glu Ala Thr Ile Trp Leu Val Glu Glu Val
130 135 140
His Arg Arg Thr Ser Ser His His His Val Ala Lys Ile Trp Gln Ser
145 150 155 160
Asp Pro Met His His Ala Phe Val Asp Ser Ile Phe Pro Lys Leu
165 170 175
<210> 98
<211> 137
<212> PRT
<213> 葡萄
<400> 98
Met Asp Ile Thr Ile Asp Gln Pro Ala Arg Gln Glu Pro Phe Glu Asp
1 5 10 15
Val Val Ile Arg Arg Ala Glu Met Tyr Gln Glu Tyr Met Lys Gln Ile
20 25 30
Pro Ile Pro Ala Arg Arg Gly Ser Leu Ile Pro Phe Asp Thr Trp Met
35 40 45
Gly Leu Ala Lys Ser Ile Lys Gln Leu Tyr Gly Gln Pro Leu His Tyr
50 55 60
Leu Thr Asn Ile Leu Val Lys Gln Trp Asp Gln Leu Arg Ile Gly Ser
65 70 75 80
Lys Asp Glu Asn Arg Ser Leu Asp Ser Ile Ile His Pro Ser Lys Ala
85 90 95
Glu Ala Ser Ile Trp Leu Ile Glu Glu Val His Arg Gln Thr Thr Ser
100 105 110
Pro His His Met Ala Lys Leu Trp Leu Ser Asp Pro Met His Tyr Ala
115 120 125
Phe Val Asp Ser Ile Phe Pro Gln Ile
130 135
<210> 99
<211> 174
<212> PRT
<213> 黄瓜
<400> 99
Met Lys Arg Pro Met Pro Trp Asn Glu Gln Val Asp Ile Ile Ser Ser
1 5 10 15
Gly Glu Ser Ser Ser Ser Asp Ser Glu Ala Ala Ile Gln Asn Asp Gly
20 25 30
Tyr Glu Phe Lys Pro Ser Leu Asp Asp Phe Lys Ile Pro Ala Lys Glu
35 40 45
Met Thr Ser Glu Gly Met Leu Met Lys Arg Ala Glu Met Tyr Gln Glu
50 55 60
Tyr Met Lys Gln Ile Gln Ile Pro Ala Gln Arg Gly Ser Val Ile Pro
65 70 75 80
Phe Thr Thr Trp Met Gly Leu Gly Lys Ser Ile Lys Gln Leu Tyr Gly
85 90 95
Gln Pro Leu His Tyr Leu Thr Asn Ile Leu Leu Lys Gln Trp Asp Gln
100 105 110
Leu Arg Leu Gly Ser Ala Asp Glu Tyr Lys Pro Leu Asp Thr Val Ile
115 120 125
His Pro Ser Lys Ala Glu Ala Thr Val Trp Leu Val Glu Glu Ile His
130 135 140
Arg Arg Thr Ser Ser His His His Val Ala Lys Leu Trp Leu Ser Asp
145 150 155 160
Pro Met His Gln Ala Tyr Val Asp Ser Ile Phe Pro Gln Leu
165 170
<210> 100
<211> 167
<212> PRT
<213> 琴叶拟南芥
<400> 100
Met Gln Ser Ser Met Thr Leu Glu Leu Arg Pro Ser Ala Asp Ser Gly
1 5 10 15
Ser Ser Asp Val Asp Ala Glu Ile Ser Asp Gly Phe Ser Pro Leu Asp
20 25 30
Asn Ser His Arg Asp Val Ala Asp Glu Gly Ser Leu Leu Arg Arg Ala
35 40 45
Glu Met Tyr Gln Glu Tyr Met Lys Gln Val Pro Ile Pro Thr Asn Arg
50 55 60
Gly Ser Leu Ile Pro Phe Thr Ser Trp Val Gly Leu Ser Ile Ser Met
65 70 75 80
Lys Gln Leu Tyr Gly Gln Pro Leu His Tyr Leu Thr Asn Val Leu Leu
85 90 95
Gln Arg Trp Asp Gln Ser Arg Phe Gly Thr Asp Ser Glu Glu Gln Ser
100 105 110
Leu Asp Ser Ile Phe His Pro Ser Lys Ala Glu Ala Thr Ile Trp Leu
115 120 125
Val Glu Glu Ile His Arg Ile Thr Ser Ser His Leu His Ile Ala Ser
130 135 140
Leu Trp Gly Ser Asp Pro Met Tyr His Ser Phe Ile Asp Pro Ile Phe
145 150 155 160
Pro Lys Ile Glu Thr Ser Ser
165
<210> 101
<211> 710
<212> PRT
<213> 拟南芥
<400> 101
Met Ala Asp Met Arg Arg Arg Asn Gly Ser Gly Gly Ser Ser Asn His
1 5 10 15
Glu Arg Asn Glu Gln Ile Leu Phe Pro Lys Pro Glu Thr Leu Asp Phe
20 25 30
Asp Leu Pro Cys Asp Thr Ser Phe Pro Gln Gln Ile Gly Asp Asn Ala
35 40 45
Ala Ser Ser Ser Gly Ser Asn Val Lys Ser Leu Leu Ile Glu Met Gly
50 55 60
Phe Cys Pro Thr Leu Val Gln Lys Ala Ile Asp Glu Asn Gly Gln Asp
65 70 75 80
Asp Phe Glu Leu Leu Leu Glu Ile Leu Thr Lys Ser Thr Glu Thr Glu
85 90 95
Pro Pro Gly Pro Ser Phe His Gly Leu Met Glu Pro Lys Pro Glu Pro
100 105 110
Asp Ile Glu Tyr Glu Thr Asp Arg Ile Arg Ile Ala Leu Leu Thr Met
115 120 125
Lys Phe Pro Glu Asn Leu Val Asp Phe Ala Leu Asp Arg Leu Gly Lys
130 135 140
Asp Thr Pro Ile Asp Glu Met Val Asp Phe Ile Val Ala Ala Gln Leu
145 150 155 160
Ala Glu Lys Tyr Ala Glu Glu Ser Glu Asp Ser Leu Asp Gly Ala Glu
165 170 175
Ile Asn Glu Glu Asp Glu Asp Val Thr Pro Val Thr Ala Arg Gly Pro
180 185 190
Glu Val Pro Asn Glu Gln Leu Phe Glu Thr Met Asp Lys Thr Leu Arg
195 200 205
Leu Leu Glu Met Gly Phe Ser Asn Asp Glu Ile Ser Met Ala Ile Glu
210 215 220
Lys Ile Gly Thr Lys Gly Gln Ile Ser Val Leu Ala Glu Ser Ile Val
225 230 235 240
Thr Gly Glu Phe Pro Ala Glu Cys His Asp Asp Leu Glu Asp Ile Glu
245 250 255
Lys Lys Val Ser Ala Ala Ala Pro Ala Val Asn Arg Thr Cys Leu Ser
260 265 270
Lys Ser Trp Arg Phe Val Gly Val Gly Ala Gln Lys Glu Asp Gly Gly
275 280 285
Gly Gly Ser Ser Ser Gly Thr Ala Asn Ile Lys Pro Asp Pro Gly Ile
290 295 300
Glu Ser Phe Pro Phe Pro Ala Thr Asp Asn Val Gly Glu Thr Ser Arg
305 310 315 320
Gly Lys Arg Pro Lys Asp Glu Asp Glu Asn Ala Tyr Pro Glu Glu Tyr
325 330 335
Thr Gly Tyr Asp Asp Arg Gly Lys Arg Leu Arg Pro Glu Asp Met Gly
340 345 350
Asp Ser Ser Ser Phe Met Glu Thr Pro Trp Met Gln Asp Glu Trp Lys
355 360 365
Asp Asn Thr Tyr Glu Phe Pro Ser Val Met Gln Pro Arg Leu Ser Gln
370 375 380
Ser Leu Gly Pro Lys Val Ala Arg Arg Pro Tyr Phe Phe Tyr Gly Gln
385 390 395 400
Leu Gly Glu Leu Ser Pro Ser Trp Trp Ser Lys Ile Ser Gly Phe Leu
405 410 415
Phe Gly Ile His Pro Glu His Val Asp Thr Arg Leu Cys Ser Ala Leu
420 425 430
Arg Arg Thr Glu Gly Tyr Leu His Asn Leu Pro Thr Val Asn Arg Phe
435 440 445
Asn Thr Leu Pro Asn Pro Arg Leu Thr Ile Gln Asp Ala Met Pro His
450 455 460
Met Arg Ser Trp Trp Pro Gln Trp Asp Ile Arg Lys His Phe Asn Ser
465 470 475 480
Gly Thr Cys Ser Asn Met Lys Asp Ala Thr Leu Leu Cys Glu Arg Ile
485 490 495
Gly Arg Arg Ile Ala Glu Cys Lys Gly Lys Pro Thr Gln Gln Asp Gln
500 505 510
Thr Leu Ile Leu Arg His Cys His Thr Ser Asn Leu Ile Trp Ile Ala
515 520 525
Pro Asn Ile Leu Ser Pro Leu Glu Pro Glu His Leu Glu Cys Ile Met
530 535 540
Gly Tyr Pro Met Asn His Thr Asn Ile Gly Gly Gly Arg Leu Ala Glu
545 550 555 560
Arg Leu Lys Leu Phe Asp Tyr Cys Phe Gln Thr Asp Thr Leu Gly Tyr
565 570 575
His Leu Ser Val Leu Lys Ser Met Phe Pro Gln Gly Leu Thr Val Leu
580 585 590
Ser Leu Phe Ser Gly Ile Gly Gly Ala Glu Ile Ala Leu Asp Arg Leu
595 600 605
Gly Ile His Leu Lys Gly Val Val Ser Val Glu Ser Cys Gly Leu Ser
610 615 620
Arg Asn Ile Leu Lys Arg Trp Trp Gln Thr Ser Gly Gln Thr Gly Glu
625 630 635 640
Leu Val Gln Ile Glu Glu Ile Lys Ser Leu Thr Ala Lys Arg Leu Glu
645 650 655
Thr Leu Met Gln Arg Phe Gly Gly Phe Asp Phe Val Ile Cys Gln Asn
660 665 670
Pro Ser Thr Pro Leu Asp Leu Ser Lys Glu Ile Ser Asn Ser Glu Ala
675 680 685
Cys Glu Phe Asp Tyr Thr Leu Phe Asn Glu Phe Ala Arg Val Thr Lys
690 695 700
Arg Val Arg Asp Met Met
705 710
<210> 102
<211> 678
<212> PRT
<213> 蓖麻
<400> 102
Met Gln Leu Ile Ser Phe Ser Asn Ser Asn Ala Val Leu Ser Trp Leu
1 5 10 15
Thr Ala Glu Ala Ser Gly His Asp Lys Met Arg Lys Val His Asn Gly
20 25 30
Glu Thr Ser Ser Asn Gln Lys Thr Thr Val Pro Lys Pro Glu Met Leu
35 40 45
Asp Phe Asp Phe Pro Ser Asp Phe Ile Tyr Ser Ser Gln Leu Gly Glu
50 55 60
Thr Ala Ala Ser Ser Ser Ala Ser Asp Val Arg Ser Phe Leu Thr Gly
65 70 75 80
Met Gly Phe Leu Pro Ser Leu Val Asp Lys Val Ile Gln Glu Asn Gly
85 90 95
Glu Asp Asn Val Asp Leu Leu Leu Glu Ile Leu Met Glu Cys Ser Asp
100 105 110
Pro Gln Lys Ser Asn Ser Gln Ser Ser Asp Ser Leu Asp Thr Leu Phe
115 120 125
Asn Asp Lys Asp Ala Ser Asn Pro Pro Glu Tyr Ser Lys Phe Gly Gln
130 135 140
Pro Lys Glu Glu Pro Asp Val Phe Gly Phe Asp Asp Ser Lys Arg Ala
145 150 155 160
Ser Leu Leu His Met Asn Phe Ser Ala Glu Glu Val Asp Phe Ala Met
165 170 175
Glu Lys Leu Gly Thr Asp Ala Pro Val Asp Glu Ile Val Asp Phe Ile
180 185 190
Thr Ala Ala Gln Val Ala Ala Asn Phe Asp Glu Glu Arg Asp Asp Glu
195 200 205
Leu Glu His Asp Val Glu Gln Asn Lys Tyr Cys Ser Arg His Ser Arg
210 215 220
Met Asn Pro Ser His Val Ala Arg Gly Gly Glu Ser Phe Asn Met Glu
225 230 235 240
Ile Arg Asp Glu Gln Arg Arg Asp Ser Tyr Gly Arg Lys Lys Pro Glu
245 250 255
Asn Leu Ser Gln Glu Ala Val Ser Arg Ser Ser Asn Leu Asn Leu Glu
260 265 270
Glu Ile His Arg Gly Lys Arg Pro Lys Gln Glu Tyr Thr Glu Gly Tyr
275 280 285
Ser Asn Thr Gln Asp Glu His Phe Gly Phe Glu Glu Asn Tyr Glu Glu
290 295 300
Glu Gly Pro Lys Pro Glu Tyr Thr Ala Asp Ser Ser Phe Ser Phe Glu
305 310 315 320
Ser Ala Trp Val Glu Glu Lys Val Asp Thr Glu Ile Thr Gly Phe Arg
325 330 335
Ile Pro Arg Glu Pro Lys Phe Lys Ser Cys Lys Ser Val Asp Arg Met
340 345 350
Val Ala Lys Pro Pro Tyr Phe Leu Tyr Gly Asn Val Ala Thr Val Ser
355 360 365
Leu Asp Thr Trp Gly Lys Ile Ser Gln Phe Leu Tyr Ala Val Glu Pro
370 375 380
Glu Phe Val Asn Thr Gln Phe Phe Ser Ala Leu Ser Arg Lys Glu Gly
385 390 395 400
Tyr Val His Asn Leu Pro Thr Glu Asn Arg Phe His Ile Leu Pro Lys
405 410 415
Pro Pro Met Ser Ile Leu Asp Ala Met Pro His Ile Lys Lys Trp Trp
420 425 430
Pro Ser Trp Asp Thr Arg Lys Gln Leu Ser Phe Ile Asn Tyr Glu Thr
435 440 445
Asn Gly Leu Ser Gln Leu Cys Asp Arg Leu Gly Arg Met Leu Thr Asp
450 455 460
Ser Arg Gly Leu Ile Ser Ser Gln Gln Lys Arg Asp Ile Leu His His
465 470 475 480
Cys His Lys Leu Asn Leu Val Trp Val Gly Leu His Lys Val Arg Pro
485 490 495
Ile Glu Pro Glu His Leu Glu Leu Ile Leu Gly Tyr Pro Leu Asn His
500 505 510
Thr Gln Thr Thr Glu Gly Ser Leu Thr Glu Arg Leu His Ala Leu Arg
515 520 525
Tyr Ser Phe Gln Ile Asp Thr Leu Gly Tyr His Leu Ser Val Leu Lys
530 535 540
Ser Met Phe Pro Glu Gly Ile Thr Val Leu Ser Leu Phe Ser Gly Ile
545 550 555 560
Gly Gly Ala Glu Val Ala Leu His Arg Leu Gly Ile His Met Lys Gly
565 570 575
Val Val Ser Val Glu Thr Ser Glu Thr Lys Arg Lys Ile Leu Arg Met
580 585 590
Trp Trp Arg Asn Ser Gly Gln Thr Gly Glu Leu Glu Gln Ile Glu Asp
595 600 605
Ile Gln Lys Leu Thr Thr Lys Lys Ile Asp Arg Leu Ile Glu Arg Phe
610 615 620
Gly Gly Phe Asp Phe Val Ile Cys Gln Ser Pro Cys Thr Cys Ala Ser
625 630 635 640
Gly Asn Pro Lys Met Glu Gln Arg Ala Asp Ala Arg Ser Ala Leu Asp
645 650 655
Phe Ser Leu Phe Cys Glu Phe Val Arg Val Leu Gln Arg Val Arg Thr
660 665 670
Gln Val Gln Arg Lys Arg
675
<210> 103
<211> 694
<212> PRT
<213> 大豆
<400> 103
Met Ala Gly Asn Pro Asn Arg Arg Glu Gly Lys Thr Val Met Val Pro
1 5 10 15
Lys Thr Glu Asn Leu Asp Tyr Glu Leu Pro Pro Tyr Thr Ser Phe Ser
20 25 30
Gly Asp Val Gly Asp Asn Val Ala Ser Ser Ser Gly Gly Lys Leu Arg
35 40 45
Ala Phe Phe Ile Gly Met Gly Phe Leu Pro Cys Leu Val Asp Lys Val
50 55 60
Ile Glu Glu Asn Gly Glu Glu Asn Ser Asp Ile Leu Leu Glu Ala Leu
65 70 75 80
Leu Arg Tyr Ser Ala Leu Gln Lys Ser Asn Ser Gln Ser Ser Val Ser
85 90 95
Leu Asp Ser Leu Phe Asp Asp Lys Asp Pro Pro Glu Ile Ser Asn Val
100 105 110
Asn Gln Ala Lys Glu Glu Pro Asp Glu Leu Ser Gly Val Val Asp Asp
115 120 125
Thr Arg Gly Ser Leu Leu Met Met Asn Phe Ser Val Glu Glu Val Glu
130 135 140
Phe Ala Ile His Lys Leu Gly Asp Glu Ala Ser Ile Pro Glu Leu Val
145 150 155 160
Asp Phe Ile Phe Ala Leu Gln Ile Ala Lys Lys Leu Lys Lys Glu Pro
165 170 175
Asp Asp Ile Thr Phe Thr Tyr Tyr Gly Arg Gly Asn Glu Val Thr Asn
180 185 190
Glu Lys Leu Phe Gly Ile Met Ala Lys Thr Leu Gln Leu Phe Glu Met
195 200 205
Gly Phe Ser Glu Asn Glu Val Ser Ser Ala Ile Asp Lys Leu Gly Ser
210 215 220
Glu Ala Pro Ile Ser Glu Leu Ala Asn Phe Ile Phe Ala Glu Gln Asn
225 230 235 240
Gly Ile Asp Tyr Val Met Glu Tyr Lys Phe Pro Thr Thr Ser Thr Tyr
245 250 255
Ser Val Gly Ile Lys Glu Glu Pro Glu Met Asp Leu Tyr Gly Thr Ala
260 265 270
Glu Val Lys Val Glu Gly Phe Ser His Glu Pro Pro Gln Ser Ser Gln
275 280 285
Val Asn Leu Glu Glu Thr Tyr Asn Asp Asp Met Val Lys Glu Glu Glu
290 295 300
Gly Ile Asp Ala Phe Pro Ser Asn Val Ser Asp Gln Tyr Leu Asp Val
305 310 315 320
Val Glu Asn Gly Arg Gly Lys Arg Pro Lys Tyr Glu His Asp Asp Asp
325 330 335
Pro Val Asn Cys Leu Glu Pro Ser Trp Val Glu Glu Arg Val Asp Ala
340 345 350
Val Val Ala Glu Met Ser Arg His Pro Lys Pro Asn Pro Ser Arg Cys
355 360 365
Leu Ser Ser Val Ala Ala Lys Pro Pro Phe Phe Leu Phe Gly Asn Val
370 375 380
Ser Asn Ile Ser Tyr Asp Ser Trp Thr Lys Met Ser Gln Phe Leu Tyr
385 390 395 400
Gly Ile Glu Pro Glu Phe Ala Asn Ala Gln Ser Phe Ser Ala Met Asp
405 410 415
Arg Ile Glu Gly Tyr Ile His Asn Leu Pro Val Glu Asn Arg Phe His
420 425 430
Ile Leu Pro Lys Pro Pro Met Thr Ile Glu Asp Ala Met Pro Gln Thr
435 440 445
Lys Lys Trp Trp Pro Pro Trp Asp Ser Arg Lys Leu Leu Ser Ser Ile
450 455 460
Tyr Cys Glu Thr Asn Gly Ile Ala Gln Thr Cys Asp Arg Leu Gly Asn
465 470 475 480
Phe Leu Ala Asp Ser Gly Gly Val Leu Thr Ser Glu Gln Gln Lys Asp
485 490 495
Ile Leu Arg Tyr Cys Arg Arg Leu Asn Leu Val Trp Ile Gly Lys Phe
500 505 510
Lys Leu Gly Pro Val Glu Pro Glu Gln Leu Glu Leu Ile Leu Gly Tyr
515 520 525
Pro Leu Asn His Thr Arg Ala Thr Glu Gly Asn Val Ala Glu Arg Leu
530 535 540
Lys Ser Leu Lys Tyr Cys Phe Gln Thr Asp Thr Leu Gly Tyr His Leu
545 550 555 560
Ser Val Leu Arg Pro Ile Phe Pro His Gly Leu Thr Met Leu Ser Leu
565 570 575
Phe Ser Gly Leu Gly Gly Ala Glu Ile Ala Leu His Arg Leu Ala Ile
580 585 590
Lys Ile Lys Ala Val Val Ser Val Glu Thr Ser Glu Thr Lys Arg Lys
595 600 605
Ile Leu Glu Lys Trp Trp Arg Gln Ser Gly Gln Thr Gly Thr Leu Val
610 615 620
Gln Ile Glu Asp Ile Gln Lys Leu Thr Ser Lys Lys Leu Glu Gly Leu
625 630 635 640
Ile Ser Lys Phe Gly Gly Phe Asp Leu Val Ile Tyr Gln Asn Pro Cys
645 650 655
Ser Tyr Ser Ser Ser Arg Leu Gln Ala Gly Val Gly Leu Ser Ala Leu
660 665 670
Asp Phe Ser Val Phe Cys Glu Cys Val Arg Val Leu Gln Arg Val Arg
675 680 685
Gly Met Tyr Gln Arg Lys
690
<210> 104
<211> 659
<212> PRT
<213> 玉蜀黍
<400> 104
Met Val Lys Val Glu Asp Gln Ala Glu Asp Gly Gly Ala Asp Ala Asp
1 5 10 15
Ala Arg Gly Leu Arg Glu Ser Ala Leu Asp Pro Leu Pro Val Ser Val
20 25 30
His Ala Ser Leu Lys Glu Glu Glu Gly Gln Pro Ser Ser Ser Ser Gly
35 40 45
Lys Leu Arg Ser Gln Phe Ile Gly Met Gly Phe Ser Ser Lys Leu Val
50 55 60
Asp Lys Val Leu Gln Arg His Gly Asp Asp Asp Ser Asn Thr Ile Leu
65 70 75 80
Glu Ser Leu Leu Ser Tyr Ser Asp Leu Gln Gln Ser Gly Ser Glu Ser
85 90 95
Ser Asp Ser Phe Gly Ser Phe Phe Asp Asn Asp Asn Glu Glu Asn Asn
100 105 110
Ser Pro Ser Glu Ser Arg Lys Gly Ile Tyr Gln Asp Ile Lys Pro Glu
115 120 125
Leu Asp Ser Phe Ser Glu Lys Trp Ser Phe Leu Leu Arg Thr Met Asn
130 135 140
Phe Ser Gln Gln Glu Val Asp Leu Ala Phe Lys Gln Leu Gly Asp Glu
145 150 155 160
Ala Pro Leu Glu Gln Leu Val Asp Phe Ile Val Ser Ala Gln Ser Gly
165 170 175
Val Pro Ser Gly Gly Leu Gln Thr Gly Asp Ala Thr Asn Glu Gly Lys
180 185 190
Thr Glu Leu Leu Tyr Gly Val Met Glu Lys Thr Leu Ser Leu Leu Gln
195 200 205
Met Gly Phe Thr Glu Glu Glu Val Ser Ser Ala Ile Asp Asn Phe Asp
210 215 220
Gln Arg Ala Thr Val Gln Glu Leu Ala Asp Ser Ile Leu Ala Arg Arg
225 230 235 240
Ile Ala Asn Ser Ile Glu Gln Lys Glu Ile Lys Val Glu Ser Asp Phe
245 250 255
Leu Asp Glu Ala Glu Thr Asp Tyr Ser Ser Tyr Gln Pro Ser Tyr Ser
260 265 270
Asp Val Ser Tyr Tyr Asp Asp Asp Asn Asn Asn Thr Arg Val Lys Arg
275 280 285
Ala Lys His Ile Phe Ile Asp Asp Ile Gly Ala Ser Ser Ser His Leu
290 295 300
Gly Asp Pro Trp Ser Met Gly Gln Arg Ala Gly Thr Asn Asp Met Ser
305 310 315 320
Val Lys Val Glu Leu Glu Ala Met Thr Pro Gly Arg Arg Ala Asn Val
325 330 335
Gln Gly Asp Leu Ala Lys Pro Pro Phe Phe Leu Tyr Gly Asn Val Val
340 345 350
Glu Val Pro Lys Asp Thr Trp His Gln Leu Lys Gln Phe Leu Tyr Asn
355 360 365
Val Glu Pro Glu Phe Val Asn Ser Gln Ser Phe Ser Ala Leu Thr Arg
370 375 380
Arg Glu Gly Tyr Ile His Asn Leu Pro Val Glu Lys Arg Ser Val Val
385 390 395 400
Val Pro Lys Ser Pro Met Thr Ile Glu Glu Ala Leu Pro Phe Thr Arg
405 410 415
Gln Trp Trp Pro Ser Trp Asp Thr Arg Lys His Ile Ser Val Ile Thr
420 425 430
Thr Glu Ala Ala Gly Ile Glu Gln Thr Cys Glu Arg Leu Gly Gly Met
435 440 445
Val Arg Glu Ser Arg Gly Val Leu Ser Gln Ala Arg Gln Met Gln Ile
450 455 460
Ile His Gln Cys Arg Val Ser Asn Leu Ile Trp Val Gly Arg Asp Arg
465 470 475 480
Leu Gly Pro Leu Glu Pro Arg Gln Val Glu Lys Ile Leu Gly Tyr Pro
485 490 495
Tyr Asn His Thr Asn Leu Phe Glu Leu Asn Gln Pro Asp Arg Leu Ala
500 505 510
Ala Met Lys Tyr Ala Phe Gln Thr Asp Thr Leu Ser Tyr Leu Leu Ser
515 520 525
Val Leu Lys Gly Lys Tyr Gln Asp Gly Ile Arg Val Leu Ser Ile Tyr
530 535 540
Ser Gly Ile Gly Gly Ala Glu Val Ala Leu His Arg Leu Gly Ile Pro
545 550 555 560
Leu Lys Cys Val Ile Ser Val Glu Glu Ser Glu Val Asn Arg Lys Ile
565 570 575
Leu Arg Arg Trp Trp Leu Lys Thr Glu Gln Thr Gly Val Leu Arg Gln
580 585 590
His Ala Gly Ile Trp Lys Leu Lys Thr His Val Ile Glu Asp Leu Val
595 600 605
Lys Glu Phe Gly Gly Phe Asp Leu Ile Ile Gly Gly Asn Tyr Thr Ser
610 615 620
Cys Lys Gly Gly Thr Thr Val Asn Thr Thr Met Gly Met Asp Ser Asn
625 630 635 640
Arg Phe Tyr Glu Tyr Asn Arg Val Val Lys Arg Val Arg Thr Ala Val
645 650 655
Gly Leu Ser
<210> 105
<211> 1032
<212> PRT
<213> 蒺藜苜蓿
<400> 105
Met Thr Gly Thr Ser Asn Gly Arg Gly Gly Lys Asn Pro Val Phe Pro
1 5 10 15
Lys Thr Glu Asp Phe Asp Tyr Glu Leu Ser Pro Tyr Thr Ala Leu Ser
20 25 30
Arg Asp Phe Arg Glu Thr Ala Ala Ser Ser Ser Gly Ser Lys Val Arg
35 40 45
Gly Phe Phe Ile Gly Met Gly Phe Leu Pro Gly Leu Val Asp Lys Val
50 55 60
Ile Glu Glu Asn Gly Thr Leu Cys Val Ser Glu Glu Asn Ser Asp Ala
65 70 75 80
Leu Leu Glu Ile Leu Leu Arg Cys Ser Thr Asn Gly Asp Ser Ser Asp
85 90 95
Ser Leu Glu Gly Ser Leu Asn Thr Asn Glu His Arg Ser Ile Pro Asn
100 105 110
Phe Phe Pro Asn Ala His Ser Lys Glu Ala Leu Gln Ile Ser Asn Leu
115 120 125
Glu Ser Ser Asp Ser Leu Asp Ser Leu Phe Asp Asp Lys Asp Ser Pro
130 135 140
Glu Val Ser Met Ile Asn Gln Pro Lys Glu Glu Val Asp Glu Ile Phe
145 150 155 160
Glu Asp Ile Glu Asp Ser Arg Gly Thr Leu Leu Met Met Asn Phe Ser
165 170 175
Ala Glu Glu Val Glu Phe Ala Ile Arg Lys Leu Gly Asn Lys Ala Pro
180 185 190
Val Pro Glu Leu Val Asp Phe Ile Phe Ala Ala Gln Ile Ala Lys Lys
195 200 205
Met Lys Lys Glu Glu Ala Glu Glu Asp Asp Ile Lys Cys Tyr Val Arg
210 215 220
Glu Lys Glu Ile Ser Asn Glu Gln Leu Phe Gly Ile Met Ala Lys Thr
225 230 235 240
Leu Gln Leu Phe Glu Met Gly Phe Ser Glu Asn Glu Ile Ser Ser Ala
245 250 255
Val Asp Lys Leu Gly Pro Asp Val Pro Ile Ser Glu Leu Ala Asn Phe
260 265 270
Ile Phe Ala Glu Gln Asn Gly Ile Glu Tyr Val Met Glu Tyr Lys Tyr
275 280 285
Pro Ser Gly Pro Ser Tyr Ser Trp Ile Lys Glu Glu Glu Gln Ser Asp
290 295 300
Met Tyr Gly Thr Ala Glu Val Lys Val Glu Asp Phe Ser His Glu Pro
305 310 315 320
Ser Gln Ser Arg Gln Val Asn Leu Gly Glu Thr Gly Thr Gly Glu Arg
325 330 335
Val Lys Glu Glu Asp Tyr Ile Asp Glu Phe Pro Asp Asp Val Lys Pro
340 345 350
Gly Phe Met Glu Asn Asp Arg Asn Lys Arg Pro Lys Tyr Glu Tyr Asp
355 360 365
Asp Asn Ser Asn Ser Ser Leu Asp Pro Tyr Trp Val Glu Glu Met Val
370 375 380
Asp Thr Val Val Ala Thr Met Ser Arg Arg Asn Lys Ser Thr Pro Ser
385 390 395 400
Arg Ser Leu Ser Ser Val Ala Ala Lys Pro Pro Phe Phe Leu Phe Gly
405 410 415
Asn Val Ser Asn Ile Thr Tyr Asp Ser Trp Lys Lys Met Ser Gln Phe
420 425 430
Leu Tyr Cys Ile Glu Pro Glu Phe Val Asn Thr Glu Leu Phe Ser Ala
435 440 445
Leu Asn Arg Ile Glu Gly Tyr Ile His Asn Leu Pro Thr Glu Asn Arg
450 455 460
Phe Gln Ile Leu Pro Lys Pro Pro Met Thr Ile Glu Asp Ala Ile Pro
465 470 475 480
Arg Thr Lys Lys Trp Trp Pro Pro Trp Asp Ser Arg Lys Lys Leu Asn
485 490 495
Cys Asn Tyr Cys Glu Thr Gly Gly Ile Thr Gln Leu Cys Asp Arg Leu
500 505 510
Gly Arg Ala Leu Ala Asn Ser Gly Gly Ile Leu Thr Ser Gln Gln Gln
515 520 525
Lys Asp Ile Leu Arg Tyr Cys Arg Gly Leu Asn Leu Val Trp Thr Gly
530 535 540
Lys Tyr Lys Leu Gly Pro Ile Glu Pro Glu His Leu Asp His Ile Leu
545 550 555 560
Gly Tyr Pro Leu Asn His Thr Arg Thr Asp Glu Cys Asp Leu Thr Glu
565 570 575
Arg Leu Gln Leu Leu Lys His Ser Phe Gln Thr Asp Thr Leu Gly Tyr
580 585 590
His Leu Ser Val Leu Lys Pro Ile Phe Pro Thr Gly Val Thr Leu Leu
595 600 605
Ser Ile Phe Ser Gly Ile Gly Gly Ala Glu Val Ala Leu His Arg Leu
610 615 620
Gly Ile Lys Ile Lys Ala Leu Val Ser Val Glu Thr Ser Ala Thr Lys
625 630 635 640
Arg Lys Ile Leu Glu Lys Trp Trp Arg Ser Ser Gly Gln Ser Gly Thr
645 650 655
Leu Val Gln Ile Glu Glu Ile Gln Lys Leu Thr Ser Lys Lys Phe Glu
660 665 670
Asn Leu Ile Asn Asn Phe Gly Val Phe Asp Leu Val Ile Tyr Gln Asn
675 680 685
Pro Cys Ser Gln Pro Ile Val Arg Pro His Gln Val Gly Gly Leu Ser
690 695 700
Ala Val Glu Phe Ser Ala Phe Cys Glu Ser Val Arg Ile Leu Gln Arg
705 710 715 720
Val Arg Ala Leu Cys Glu Arg Arg Glu Met Thr Val Ser Leu Pro Val
725 730 735
Leu Ser Glu Val Ile Trp Lys Val Leu Val Gly Leu Val Lys Gln Gly
740 745 750
Asp Asp Ser Phe Thr Ser Ser Val Val Arg Ser Asp Met Glu Gly Ser
755 760 765
Met Val Asp Arg Cys Pro Arg Tyr Asp Ile Lys Lys Val Gly Val Leu
770 775 780
Arg Trp Gly Tyr Cys Arg Arg Glu Glu Gly Lys Asn Ala Asn Glu Ser
785 790 795 800
Gly Glu Ala Thr Ala Lys Ser Ala Lys Gly Val Ile Val Ile Glu Gly
805 810 815
Leu Ile Thr Ser Leu Ala Leu Ala Leu Gly Leu Glu Ser Glu Leu Ala
820 825 830
Asn Leu Val Ala Leu Glu Asp Cys Thr Gln Leu Asp Leu His Ser Cys
835 840 845
Met Asn Gln Lys Phe Val Gln Val Lys Gly Lys Ile Gln Phe Tyr Leu
850 855 860
Met Ile Lys Asn Glu Leu Val Arg Ser Ile Val Leu Pro Asn Arg Glu
865 870 875 880
Gln Ile Asn Val Arg Asn Glu Asn Asn Trp Leu Phe Asp Leu Glu Ala
885 890 895
Pro Glu Ser Pro Leu Ala Ala His Pro Ala Ala Ala Tyr Arg Ala Asp
900 905 910
Asp Ala His Glu Gly Gly Gly Thr Asp Asp Glu Tyr Asp Arg Gln Glu
915 920 925
Arg Leu Ser Pro Ala His Ser Tyr Pro His Leu Thr Pro Ser Pro Asp
930 935 940
Arg His Ile Pro Pro His Thr Gln Ala Pro Asp Tyr Thr Pro His Leu
945 950 955 960
Pro His Ile Cys Thr Leu Val Ser Thr Ser Tyr Thr Ser Gln Ser Ser
965 970 975
Val Glu Tyr Glu Leu Asn Ala Leu Arg Pro Glu Val Ala Asn Leu Arg
980 985 990
Ala Thr Gln Gln Glu Arg Ser Asp Arg Glu Thr Glu Arg Asp His Gln
995 1000 1005
Met Arg Val Asn Asn His Met Ile Arg Gln Met Phe Thr Trp Ile Thr
1010 1015 1020
Arg His Asp Met Pro Pro Ala Glu
1025 1030
<210> 106
<211> 576
<212> PRT
<213> 两色蜀黍
<400> 106
Met Ala Asp Arg Val Gly Gly Ser Asp Asp Gly Asp Lys Ser Glu Trp
1 5 10 15
Asn Ser Asp Gly Glu Val Ala Arg Ser Leu Asp Gly Ala Gly Ser Pro
20 25 30
Ile Ala Pro Ser Arg Asn Lys Leu Lys Asp Ala Pro Gly Pro Ser Leu
35 40 45
Leu Val Asn Gly Ala Gly Pro Ser Ala Ser Leu Val Glu Asp Phe Val
50 55 60
Gly Met Gly Leu Ile Lys Gly Asn Gly Asp Gly Gly Ala Glu Ser Leu
65 70 75 80
Leu Glu Leu Leu Leu Ala Tyr Lys Ala Ile Gly Asn Asp Pro Ser Leu
85 90 95
Asp Lys Ser Ser Ala Ser Val Cys Gly Pro Arg Thr Ile Cys Asp Asp
100 105 110
Lys Asp Ile Leu Ala Asn Trp Asp Ala His Asp Ala Ser Thr Ser Ser
115 120 125
Asp Arg Asp His Ala Ser Asp Asp Ser Gly Asp Glu Asp Phe Leu Gln
130 135 140
Gly Leu Ser Gln Lys Asp Glu Lys Ile Glu Ser Leu Val Arg Met Gly
145 150 155 160
Phe Pro Lys Asp Glu Ala Glu Met Ala Ile Val Arg Cys Gly Gln Asp
165 170 175
Ala Pro Met Ser Val Leu Ile Asp Leu Ile Tyr Ser Ser Glu Ala Ser
180 185 190
Glu Asp Gly Tyr Tyr Gly Asn Phe Ser Gly His Glu Asp Asp Ser Phe
195 200 205
Gly Gly Arg Lys Glu Lys Arg Lys Arg Ser Glu Asp Ala Glu Gln Gly
210 215 220
Ser Ser His Asp Glu Pro Met Pro Leu Pro Asn Pro Met Val Gly Phe
225 230 235 240
Asn Leu Pro Asn Val Ser Leu Arg Ser Val Asp Arg Ser Leu Pro Ser
245 250 255
Lys Ala Ile Gly Pro Pro Phe Phe Tyr Tyr Glu Asn Val Ala Ile Ala
260 265 270
Pro Lys Gly Val Trp Thr Thr Ile Ser Gln Phe Leu Tyr Asp Ile Gln
275 280 285
Pro Glu Phe Val Asp Ser Arg Phe Leu Cys Ala Ala Ala Arg Lys Arg
290 295 300
Gly Tyr Val His Asn Leu Pro Ile Glu Asn Arg Ser Pro Leu Leu Pro
305 310 315 320
Leu Pro Pro Lys Thr Ile Phe Glu Ala Phe Pro His Thr Lys Lys Trp
325 330 335
Trp Pro Ser Trp Asp Pro Arg Lys Gln Phe Asn Cys Leu Leu Thr Ser
340 345 350
Met Ala Lys Pro Lys Leu Thr Glu Gln Ile His His Ala Leu Ala Lys
355 360 365
Cys Lys Asp Pro Pro Pro Arg Arg Val Gln Lys Tyr Val Leu Glu Thr
370 375 380
Cys Arg Thr Ala Asn Leu Val Trp Val Gly Leu Asn Lys Val Ala His
385 390 395 400
Leu Glu Pro Asp Glu Met Glu Phe Leu Leu Gly Phe Pro Lys Asp His
405 410 415
Thr Arg Gly Ile Gly Arg Pro Glu Arg Phe Lys Ser Leu Gly Asn Ser
420 425 430
Phe His Val Asp Thr Val Ala Tyr His Leu Ser Ala Leu Arg Asp Met
435 440 445
Phe Pro His Gly Met Asn Val Leu Ser Leu Phe Ser Gly Ile Gly Gly
450 455 460
Ala Glu Val Ala Leu His Arg Leu Asp Ile Arg Met Lys Thr Val Val
465 470 475 480
Ser Val Glu Ile Ser Glu Thr Asn Arg Phe Val Leu Arg Thr Trp Trp
485 490 495
Asn Gln Thr Gln Thr Gly Thr Leu Ile Glu Ile Thr Asp Val Gln Ser
500 505 510
Leu Thr Thr Glu Arg Leu Glu Ser Cys Ile Arg Arg Ile Gly Gly Phe
515 520 525
Asp Leu Val Ile Gly Gly Ser Pro Cys Asn Asn Leu Ala Gly Arg Asn
530 535 540
Arg Phe His Arg Asp Gly Leu Glu Gly Glu Gln Ser Ser Leu Phe Tyr
545 550 555 560
His Tyr Tyr Arg Ile Leu Asp Thr Val Lys Ser Ile Met Gly Arg Met
565 570 575
<210> 107
<211> 680
<212> PRT
<213> 稻
<400> 107
Met Val Lys Val Glu Asp Asp Val Glu Gly Ser Gly Ile Asn Ala Ser
1 5 10 15
Val Gly Asp Leu Arg Asp Ala Ala Val Asn Pro Gln Pro Ala Leu Leu
20 25 30
Arg Ala Thr Val Lys Glu Glu Glu Gly Gln Pro Ser Ser Ser Ser Ser
35 40 45
His Val Arg Ser Gln Phe Ile Gly Met Gly Phe Ser Pro Met Leu Val
50 55 60
Asp Arg Val Leu Gln Lys His Gly Asp Arg Asp Ser Asp Thr Ile Leu
65 70 75 80
Glu Ala Leu Leu Ser Gln Ser Ala Leu Gln Lys Ser Gly Ser Glu Ser
85 90 95
Gly Ser Leu Gly Asp Leu Phe Asp Ser Asp Asn Glu Glu Asn Ser Ser
100 105 110
His Phe Ala Pro Arg Lys Glu Val Ile Gln Asp Ile Lys Val Glu Ala
115 120 125
Asp Ser Ser Ser Glu Lys Arg Ser Tyr Leu Leu Ser Thr Met Asn Phe
130 135 140
Ser Gln Arg Glu Val Asp Leu Ala Leu Asn Gln Leu Gly Glu Glu Ala
145 150 155 160
Ser Leu Glu Gln Leu Val Asp Phe Ile Val Thr Gly Gln Val Ser Gly
165 170 175
Cys Ser Gly Gly Asn Glu Asn Gly Asp Ala Ser Asn Glu Val Lys Asp
180 185 190
Glu Ser Leu Phe Gly Val Met Asp Lys Thr Leu His Leu Leu Gln Met
195 200 205
Gly Phe Thr Glu Glu Glu Val Ser Ser Val Ile Asp Lys Ala Gly Pro
210 215 220
Glu Ala Thr Val Leu Glu Leu Ala Asp Thr Ile Phe Ala Arg Arg Ile
225 230 235 240
Ala Ser Ser Ile Glu Gln Lys Glu Val Lys Val Glu Pro Asp Phe Leu
245 250 255
Asp Glu Thr Glu Thr Ser Tyr Ser Ala Tyr His Pro Ser Asn Ser Gly
260 265 270
Leu Arg Tyr Tyr Asp Asp Asp His Asp Asn Ile Arg Ile Lys Arg Ala
275 280 285
Lys His Met Phe Ile Asp Asp Ser Ala Gly Ser Ser Ser Arg Ala Gly
290 295 300
Asn Gln Pro Asn Leu Asp Pro Trp Leu Lys Asp His Arg Ala Thr Thr
305 310 315 320
Ser Asp Gly Ser Val Lys Glu Glu Phe Asp Ala Met Thr Pro Gly Ile
325 330 335
Arg Arg Asn Val Arg Ser Asp Val Ala Asn Pro Pro Tyr Phe Leu Tyr
340 345 350
Gly Asn Val Val Glu Ile Pro Lys Ala Thr Trp Arg Gln Leu Ser Glu
355 360 365
Phe Leu Tyr Asn Val Glu Pro Glu Phe Val Asn Ser Gln Phe Phe Ser
370 375 380
Ala Leu Ser Arg Lys Glu Gly Tyr Ile His Asn Leu Pro Thr Glu Gly
385 390 395 400
Arg Arg Asn Leu Val Pro Arg Ser Pro Met Thr Ile Glu Glu Ala Phe
405 410 415
Pro Phe Thr Arg Gln Cys Trp Pro Ser Trp Asp Thr Arg Lys Gln Leu
420 425 430
Asn Ser Val Ala Thr Glu Val Ala Gly Ile Glu Gln Leu Cys Glu Arg
435 440 445
Leu Gly Lys Met Val Arg Asp Ser Gly Gly Tyr Leu Ser Gln Glu Lys
450 455 460
Lys Thr His Ile Met His Gln Cys Lys Leu Ala Asn Leu Ile Trp Val
465 470 475 480
Gly Pro Asp Arg Leu Ser Pro Leu Asp Pro Gln Gln Val Glu Arg Ile
485 490 495
Leu Gly Tyr Pro Arg Lys His Thr Asn Leu Phe Gly Leu Asn Pro Gln
500 505 510
Asp Arg Ile Glu Ala Met Arg Tyr Ser Phe Gln Thr Asp Thr Leu Gly
515 520 525
Tyr Leu Leu Ser Val Leu Lys Asp Leu Tyr Pro Asp Gly Leu Arg Val
530 535 540
Leu Ser Ile Tyr Ser Gly Ile Gly Gly Ala Ala Ile Ala Leu His Arg
545 550 555 560
Leu Gly Ile Pro Leu Gln Cys Val Val Ser Val Asp Gln Ser Asp Thr
565 570 575
Asn Arg Lys Ile Leu Arg Arg Trp Trp Ser Asn Thr Glu Gln Lys Gly
580 585 590
Gln Leu Arg Gln Ile Asn Thr Ile Trp Lys Leu Lys Ile Asn Val Leu
595 600 605
Glu Asp Leu Val Lys Glu Phe Gly Gly Phe Asp Ile Ile Ile Gly Gly
610 615 620
Asn Phe Ser Ser Cys Lys Gly Gly Thr Thr Val Asn Ser Ser Met Gly
625 630 635 640
Met Asp Ser Asn Gln Phe Phe Glu Tyr Val Arg Val Val Gln Arg Val
645 650 655
Lys His Ile Met Gly Arg Leu Gln Val Arg Ala His Glu Ser Ala Arg
660 665 670
His Arg His Arg Ser Pro Ser Asn
675 680
<210> 108
<211> 660
<212> PRT
<213> 二穗短柄草
<400> 108
Met Val Lys Thr Glu Asp Gln Asp Glu Asp Glu Gly Val Ser Ala Ser
1 5 10 15
Val Gly Ala Leu Arg Asp Gly Thr Val Asp Pro Gln Pro Asp Thr Ala
20 25 30
His Ala Gly Val Lys Ala Glu Glu Asp Gly Gln Pro Ser Ser Ser Ser
35 40 45
Ser Tyr Ser Lys Phe Ile Gly Met Gly Phe Pro Pro Thr Leu Val Asp
50 55 60
Lys Met Leu Gln Lys His Gly Asp Lys Asp Phe Asn Ala Ile Leu Glu
65 70 75 80
Ser Leu Leu Phe His Ser Ser Leu Thr Lys Ser Gly Ser Glu Ser Ser
85 90 95
Ser Ser Leu Gly Ser Leu Phe Asp Ser Asp Ser Glu Glu Asn Ile Ser
100 105 110
Arg Leu Gly Ser Met Lys Glu Pro His Glu Glu Ile Lys Pro Glu Pro
115 120 125
Asp Ser Phe Ser Glu Arg Arg Ser Tyr Leu Leu Ser Ala Met Asn Phe
130 135 140
Ser Gln Gln Glu Val Asp Trp Ala Phe Asn Gln Leu Gly Glu Glu Ala
145 150 155 160
Pro Leu Asp Gln Leu Val Asp Thr Ile Val Thr Ala Gln Val Ala Gly
165 170 175
Phe Thr Gly Gly Asn Glu Asn Val Asp Ala Thr Thr Glu Gly Lys Ala
180 185 190
Glu Ser Leu Phe Gly Val Met Glu Lys Thr Leu His Leu Leu Gln Met
195 200 205
Gly Phe Thr Glu Glu Glu Val Ser Gly Ala Ile Asp Ser Ser Gly Gln
210 215 220
Glu Ala Thr Val Gln Glu Leu Ala Asp Ser Ile Phe Ala Arg Arg Ile
225 230 235 240
Asp Asn Ser Ile Lys Gln Thr Glu Val Lys Ile Glu Ser Asp Phe Leu
245 250 255
Gly Gly Thr Glu Asn Gln His Ser Thr Cys His Gln Arg Leu Arg Tyr
260 265 270
Tyr Asp Asp Glu Asp Asp Lys Ile Gly Val Lys Arg Ala Lys Ser Ile
275 280 285
Phe Thr Asp Asn Ser Ser Gly Ala Ser Ser Ser Arg Pro Gly Asn Gln
290 295 300
Pro Ser Leu Thr Pro Trp Leu Ser Gly Cys Thr Gly Ser Val Ser Asn
305 310 315 320
Gly Tyr Val Lys Glu Glu Phe Asp Ala Met Ala Ser Gly Pro Arg Pro
325 330 335
Asp Val Arg Pro Glu Ile Ala Lys Pro Pro Tyr Phe Leu Tyr Gly Asn
340 345 350
Val Val Asp Ile Pro Lys Gly Thr Trp His Gln Leu Ser Asp Phe Leu
355 360 365
Phe Asn Val Glu Pro Glu Phe Leu Asn Ser Gln Tyr Phe Ser Ala Val
370 375 380
Met Arg Arg Glu Gly Tyr Leu His Asn Leu Pro Met Glu Thr Arg His
385 390 395 400
Ile Val Val Pro Lys Ser Pro Met Thr Ile Glu Asp Ala Leu Pro Phe
405 410 415
Thr Arg Gln Trp Trp Pro Ser Trp Asp Thr Arg Lys His Ile Gly Val
420 425 430
Val Thr Leu Asp Val Ala Glu Ile Glu Gln Met Cys Glu Lys Leu Gly
435 440 445
Lys Ile Met Thr Asp Ser Arg Gly Val Leu Ser His Glu Lys Gln Ala
450 455 460
His Ile Met Gln Gln Cys Lys Met Ser Asn Leu Ile Trp Ile Gly Lys
465 470 475 480
Asp Arg Leu Ser Pro Leu Glu Pro His Gln Leu Glu His Ile Leu Gly
485 490 495
Tyr Pro Arg Asn His Thr Glu Gln Phe Glu Leu Asn Thr Pro Asp Arg
500 505 510
Leu Ala Ala Met Lys Tyr Thr Phe Gln Thr Asp Val Leu Ala Tyr Leu
515 520 525
Leu Ser Val Leu Lys Ser Lys Tyr Pro Asn Gly Leu Arg Leu Leu Ser
530 535 540
Ile Tyr Ser Gly Leu Gly Gly Ala Glu Val Ala Leu Asp Arg Leu Gly
545 550 555 560
Ile Pro Ile Lys Cys Ile Val Ser Val Glu Glu Ser Asp Val Asn Arg
565 570 575
Lys Ile Leu Arg Lys Trp Trp Arg Arg Thr Asn Gln Ala Gly Glu Leu
580 585 590
Arg Gln Phe Val Gly Ile Trp Lys Leu Lys Thr Ile Val Leu Glu Asp
595 600 605
Leu Val Lys Glu Phe Gly Gly Phe Asp Leu Ile Ile Gly Gly Asn Tyr
610 615 620
Ser Ser Cys Arg Gly Gly Arg Thr Ile Asn Ala Thr Met Gly Met Asp
625 630 635 640
Ser Asn Lys Phe Tyr Glu Tyr Ala Arg Val Val Lys Ile Val Arg Ala
645 650 655
Leu His Asn Ser
660
<210> 109
<211> 661
<212> PRT
<213> 毛果杨
<400> 109
Met Gly Tyr Ser Pro Ser Leu Val Asn Arg Val Ile Glu Glu Asn Gly
1 5 10 15
Glu Asp Asn Val Asp Leu Leu Leu Glu Ile Leu Met Glu Cys Ser Gly
20 25 30
Leu Gln Lys Pro Asn Ser Gln Ser Ser Asp Ser Leu Asp Cys Leu Phe
35 40 45
Asp Asp Arg Gly Glu Ser Ser Pro Pro Lys Tyr Ser Thr Val Thr Gly
50 55 60
Val Lys Glu Glu Pro Asp Val Phe Asp Glu Val Tyr Asp Asp Lys Arg
65 70 75 80
Val Ser Leu Leu Lys Met Asn Phe Pro Ala Lys Glu Val Glu Leu Ala
85 90 95
Met Asp Lys Leu Gly Glu Asn Ala Pro Ile Asn Glu Ile Ile Asp Phe
100 105 110
Ile Ile Ala Ala Gln Ile Ala Asn Asn Leu Asp Arg Glu Thr Glu Asp
115 120 125
Met Pro Asp Ile Asp Ala Glu Asn Lys Glu Asp Val Asn Asp Glu Thr
130 135 140
Leu Tyr Gly Thr Met Asp Lys Thr Leu Cys Leu Leu Asn Met Gly Phe
145 150 155 160
Ser Glu Asn Glu Val Ser Leu Ala Ile Asp Lys Phe Gly Ser Glu Val
165 170 175
Pro Val Thr Glu Leu Ala Asn Ala Ile Cys Ala His Gln Leu Gly Glu
180 185 190
Thr Tyr Val Ile Lys Lys Lys Tyr Ser Glu Asn Ser Thr Ala Ser Cys
195 200 205
Ser Ser Ala Ala Glu Asp Ser Arg Ser Phe Gly Val Glu Thr Glu Asn
210 215 220
Asn Thr Arg His His Ser Phe Ser Trp Val Lys Ser Glu Thr Glu Asp
225 230 235 240
Cys Arg Arg Asp Ala Ile Leu Gln Ser Arg Asp Met Asn Thr Lys Glu
245 250 255
Thr Arg Lys Gly Lys Arg Pro Arg Gln Glu His Ile Glu Gly Tyr Gln
260 265 270
Glu Ala Gln Pro Arg His Asp Ser Leu Glu Glu Asn Cys Ala Gly Glu
275 280 285
Gln Pro Lys Gln Glu Tyr Asp Tyr Gly Ser Ser Ser Tyr Phe Glu His
290 295 300
Glu Trp Val Glu Glu Lys Val Asn Ser Asp Thr Thr Thr Phe Gly Met
305 310 315 320
Pro Lys Pro Phe Lys Cys Asn Pro Cys Lys Ile Leu Asp Gln Ile Ala
325 330 335
Ala Lys Pro Pro Tyr Phe Phe Tyr Gly Asn Val Ala Thr Ala Ser Ser
340 345 350
Asp Thr Trp Gly Lys Ile Ser Gln Phe Leu Tyr Gly Ile Glu Pro Glu
355 360 365
Phe Val Asp Thr Gln Phe Phe Ser Ala Leu Ser Arg Arg Glu Gly Tyr
370 375 380
Ile His Asn Leu Pro Thr Glu Asn Arg Ser His Ile Leu Pro Lys Pro
385 390 395 400
Pro Ile Thr Leu Glu Asp Leu Met Pro Ser Thr Lys Lys Trp Trp Pro
405 410 415
Ser Trp Asp Ala Arg Lys Lys Met Ser Cys Arg Asn Phe Asp Ser Ser
420 425 430
Gly Ser Ser Gln Leu Cys Asp Met Leu Gly Arg Met Leu Asp Asp Ser
435 440 445
Arg Gly Leu Leu Ser Ala Glu Gln Gln Arg Asp Leu Leu Arg His Cys
450 455 460
Gln Ala Leu Asn Leu Met Trp Val Gly Pro Asn Lys Leu Ser Pro Leu
465 470 475 480
Glu Ser Ala His Leu Glu Lys Ile Leu Gly Tyr Pro Leu Asn His Thr
485 490 495
Leu Ile Ala Asp Tyr Pro Leu Thr Glu Arg Met Tyr Ser Leu Arg Tyr
500 505 510
Ser Phe Gln Thr Asp Thr Leu Gly Tyr His Leu Ser Val Leu Lys Ser
515 520 525
Ile Phe Pro Gln Gly Ile Thr Val Leu Ser Leu Phe Ser Gly Ile Gly
530 535 540
Gly Ala Glu Ile Thr Leu His Arg Leu Gly Ile His Leu Lys Gly Val
545 550 555 560
Val Ser Val Glu Thr Ser Glu Thr Asn Arg Arg Val Leu Lys Arg Trp
565 570 575
Trp Tyr Ser Ser Gly Gln Thr Gly Arg Leu Glu Gln Ile Glu Asp Ile
580 585 590
Arg Lys Leu Thr Ser Ser Thr Val Glu Arg Leu Val Glu Asn Phe Val
595 600 605
Cys Phe Asp Phe Val Ile Cys Gln Asn Ser Phe Thr Arg Pro Ser Lys
610 615 620
Ile Pro Gly Val Gly Ser Gly Leu Glu Ser Gln His Phe Phe Asp Phe
625 630 635 640
Thr Leu Phe Asn Glu Phe Val Arg Val Leu Gln Arg Val Arg Ser Ala
645 650 655
Ile Glu Arg Lys Arg
660
<210> 110
<211> 712
<212> PRT
<213> 葡萄
<400> 110
Met Gly Glu Ile Ala Asp Tyr Ser Asp Ser Lys Ser Ser Ser Glu Thr
1 5 10 15
Val Gly Glu Arg Gly Thr Met Ile Lys Val Glu Pro Leu Glu Leu Asn
20 25 30
Leu Pro Thr Glu Asn Met Cys Ala Arg His Ile Gly His Asn Gly Ala
35 40 45
Ser Ser Ser Gly Ser Lys Leu Arg Ser Ser Phe Ile Gly Met Gly Phe
50 55 60
Leu Pro Ser Leu Val Asp Lys Val Ile Glu Glu Lys Gly Glu Asp Asp
65 70 75 80
Val Asp Leu Leu Leu Glu Thr Leu Cys Ala Asn Ala Ala Leu Gln Lys
85 90 95
Ser Ile Ser Glu Ser Ser Asp Ser Leu Asp Ser Phe Leu Asn Asp Glu
100 105 110
Asp Thr Asn Asn Pro Pro Lys Phe Ser Ala Gly Phe His Pro Lys Glu
115 120 125
Glu Pro Asp Val Ser Ser Gly Val Asn Asp Asp Lys Arg Ala Thr Leu
130 135 140
Val Met Met Lys Phe Ser Val Asp Glu Val Glu Phe Ala Met Asp Lys
145 150 155 160
Leu Gly Glu Gly Ala Pro Val Asn Glu Leu Val Asp Phe Ile Ile Ala
165 170 175
Ala Gln Ile Ala Gly Ser Ser Lys Met Asp Ala Asp Asp Pro Thr Tyr
180 185 190
Gly Asn Glu Glu Arg Lys Glu Asp Cys Asn Asn Glu Ala Leu Phe Gly
195 200 205
Thr Met Asp Lys Thr Leu Arg Leu Leu Glu Met Gly Phe Ser Glu Asn
210 215 220
Glu Ile Ser Ser Ala Ile Glu Lys Phe Gly Ser Glu Val Pro Ile Ser
225 230 235 240
Glu Leu Ala Asp Ser Ile Phe Ala Gly Gln Ile Ala Glu Lys Asp Lys
245 250 255
His Ser Ser Ala Thr Phe Arg Leu Asn His Ser His Thr Gly Asn Asp
260 265 270
Tyr Arg Ser Phe Gly Lys Gly Met Glu Asp Gly Leu Lys Arg Arg Ser
275 280 285
Ser Gly Thr Phe Met Val Lys Thr Glu Glu Tyr Ser Pro Glu Ala Val
290 295 300
Ser Gln Leu Arg Ala Val Asp Ile Gly Asp Asp His Lys Gly Lys Arg
305 310 315 320
Pro Lys Gln Glu Ser Leu Asp Asp Ser Ser Ala Tyr Ile Gly Pro Thr
325 330 335
Trp Leu Glu Gly Arg Lys Gly Asp His Lys Phe Ser Ser Cys Arg Met
340 345 350
Pro Leu Pro Arg Arg Asp Leu Asn Gln Val Ala Gly Gln Leu Asn Lys
355 360 365
Phe Gly Met Ser Ser Ile Ser Lys Pro Met Pro Cys Lys Ser Leu Asp
370 375 380
Gln Leu Val Ala Lys Pro Pro Tyr Phe Phe Tyr Gly Asn Val Thr Asn
385 390 395 400
Val Ser His Asp Ser Trp Asn Lys Ile Ser Gln Phe Leu Tyr Ala Leu
405 410 415
Glu Pro Glu Phe Val Asn Thr Gln Phe Phe Ser Ala Leu Asn Arg Lys
420 425 430
Glu Gly Tyr Ile His Asn Leu Pro Ser Glu Asn Arg Phe His Ile Leu
435 440 445
Pro Lys Pro Pro Met Thr Ile Glu Glu Val Ile Pro Tyr Thr Lys Lys
450 455 460
Trp Trp Pro Ser Trp Asp Thr Arg Lys Gln Leu Ser Cys Ile Ser Ser
465 470 475 480
Glu Thr Ser Gly Ile Ser Gln Leu Cys Asp Arg Leu Gly Lys Ile Leu
485 490 495
Val Asp Ser Arg Gly Leu Leu Ser Phe Glu Gln Gln Arg Asp Ile Leu
500 505 510
His His Cys Arg Thr Leu Asn Leu Val Trp Val Gly Gln Cys Lys Leu
515 520 525
Ser Pro Ile Glu Pro Glu His Leu Glu Arg Ile Leu Gly Tyr Pro Leu
530 535 540
Asn His Thr Arg Val Pro Glu Tyr Ser Leu Ile Glu Arg Leu Gln Ser
545 550 555 560
Leu Arg His Cys Phe Gln Ile Asp Thr Leu Gly Tyr His Leu Ser Val
565 570 575
Leu Lys Ser Met Phe Pro Lys Gly Leu Thr Met Leu Ser Leu Phe Ser
580 585 590
Gly Ile Gly Gly Ala Glu Leu Thr Leu His Gln Leu Gly Ile His Leu
595 600 605
Lys Gly Val Val Ser Val Glu Ile Ser Glu Thr Lys Arg Asn Ile Leu
610 615 620
Lys Lys Trp Trp His Asn Thr Gly Gln Thr Gly Glu Leu Val Gln Ile
625 630 635 640
Asp Asp Ile Gln Lys Leu Ala Ser Ser Lys Leu Glu Ser Leu Ile Glu
645 650 655
Lys Phe Gly Gly Phe Asp Phe Val Ile Cys Gln Asn Pro Cys Thr Tyr
660 665 670
Ser Ser Arg Asn Ser Lys Met Val Ala Asp Gly Asp Ser Leu Thr Gly
675 680 685
Phe Asp Phe Ser Leu Phe Cys Glu Phe Val Arg Val Leu His Arg Val
690 695 700
Arg Ser Thr Met Glu Arg Arg Arg
705 710
<210> 111
<211> 724
<212> PRT
<213> 黄瓜
<400> 111
Met Ala Ser Asn Lys Pro Ile Val Pro Lys Glu Glu Val Phe Asp Phe
1 5 10 15
Arg Leu Pro Pro Asp Arg Met Tyr Ser Arg His Val Gly Asp Ser Gly
20 25 30
Ala Ser Ser Ser Gly Ser Asn Val Arg Thr Phe Phe Ile Asp Met Gly
35 40 45
Phe Leu Pro Ser Leu Val Asp Ser Val Ile Glu Lys Asn Glu Gln Lys
50 55 60
Ser Ile Pro Gln Ser Ser Asp Ser Leu Glu Gly Leu Gln Ser Gly Lys
65 70 75 80
Met Gly Ser Asn Pro Pro His Val Ser Thr Val Cys His Lys Gln Val
85 90 95
Gln Ala Ala Gln Thr Ser Lys Ser Glu Ser Ser Asp Ser Leu Asp Ser
100 105 110
Leu Phe Asp Asp Lys Asp Ala His Asn Glu Ile Ser Ser Val Ile Pro
115 120 125
Lys Glu Glu Ala Asp Asp Tyr Tyr His Ile Ser Asp Thr Asn Lys Ala
130 135 140
Ser Leu Leu Val Met Asn Phe Ser Ala Asp Glu Val Asp Phe Ala Ile
145 150 155 160
Asp Lys Leu Gly Gly Asp Ala Pro Leu Asn Glu Leu Val Asp Phe Ile
165 170 175
Ile Ala Ala Gln Ile Ala Ile Lys Leu Glu Lys Glu Thr Asp Asp Ala
180 185 190
Phe Cys Arg Asn Glu Leu Lys Lys Glu Glu Asn Asp Glu Thr Leu Phe
195 200 205
Val Thr Met Glu Lys Thr Leu Arg Leu Leu Glu Met Gly Phe Ser Glu
210 215 220
Asn Glu Val Ser Leu Ala Ile Glu Lys Phe Gly Ser Glu Thr Gln Val
225 230 235 240
Ser Glu Leu Ala Asp Ser Ile Val Thr Gly Arg Ile Ala Ser Asp Tyr
245 250 255
Pro Gly Asp Val Lys Cys Ser Pro Ser Ser Phe Gly Ile Gly Gly Leu
260 265 270
Tyr Thr Arg Glu Asp Tyr Val Thr Lys Val Lys Ala Glu Glu Ser Ser
275 280 285
Ser Ala Val Gly Pro Leu Pro Arg Asn Val Asn Ile Glu Ala Ile Gln
290 295 300
Lys Gly Lys Arg Pro Lys Glu Glu Asn Met Asp Asp Leu Leu Asn Pro
305 310 315 320
Thr Thr Arg Leu Asn Lys His Lys Gly Lys Arg Pro Lys Gln Glu Tyr
325 330 335
Ala Asp Asp Leu Gly Ser Leu Tyr Gly Pro Gly Trp Val Glu Ser Lys
340 345 350
Val Asn Pro Asp Ile Thr Ser Phe Asp Ile Pro Pro Ser Ser Arg Leu
355 360 365
Asn Leu Ser Arg Ser Leu Asp Lys Leu Val Ala Lys Pro Pro Cys Pro
370 375 380
Pro Leu Lys Ser Asn Pro Ser Arg Ala Leu Glu Lys Val Val Thr Lys
385 390 395 400
Pro Pro Phe Phe Leu Tyr Gly Asn Val Leu Asp Ile Ser Arg Asp Ser
405 410 415
Trp Ala Lys Val Ser Lys Phe Leu Tyr Ala Val Glu Pro Glu Phe Val
420 425 430
Asp Thr Arg Ser Phe Ser Ala Leu Ser Arg Thr Glu Gly Tyr Val His
435 440 445
Asn Leu Pro Cys Glu Asn Arg Phe His Ile Ile Pro Leu Pro Pro Met
450 455 460
Thr Ile Gln Asp Ala Thr Arg Thr Lys Lys Trp Trp Pro Ser Trp Asp
465 470 475 480
Thr Arg Lys Tyr Leu Ser Cys Ile Asn Ser Glu Thr Arg Gly Val Pro
485 490 495
Gln Leu Cys Asp Arg Leu Thr Lys Thr Leu Thr Asp Ser Gly Gly His
500 505 510
Pro Ser Ser His Glu Glu Arg Asp Ile Leu His His Cys Ile Ala Leu
515 520 525
Asn Leu Ile Trp Val Ser Gln Phe Lys Leu Ala Pro Val Glu Pro Glu
530 535 540
Gln Leu Glu Cys Val Leu Gly Tyr Pro Val Asn His Thr Gln Asp Ala
545 550 555 560
Glu Ser Ser Ser Ile Glu Arg Leu Gln Tyr Leu Lys Tyr Cys Phe Gln
565 570 575
Thr Asp Ala Leu Gly Tyr His Leu Ser Val Leu Lys Ser Met Phe Pro
580 585 590
Glu Gly Leu Val Val Leu Ser Ile Phe Ser Gly Ile Gly Gly Ala Glu
595 600 605
Ile Ala Leu His Arg Leu Gly Ile His Leu Lys Val Val Val Ser Val
610 615 620
Glu Ser Ser Ala Ala Lys Arg Arg Ile Leu Lys Lys Trp Trp His Ser
625 630 635 640
Ser Gly Gln Thr Gly Glu Leu Glu Gln Ile Glu Asp Ile Gln Lys Leu
645 650 655
Thr Ser Ile Lys Ile Asn Asn Trp Ile Thr Lys Tyr Gly Gly Phe Asp
660 665 670
Leu Val Ile Cys Gln Asn Pro Cys Ser Arg Cys Leu Ser Ser Ser Lys
675 680 685
Leu Asn Gln Ser Gly Asp Ala Glu Gly Ile Ala Ser Phe Asp Phe Ser
690 695 700
Ile Phe Tyr Glu Phe Val Arg Val Leu Gln Ser Val Arg Asn Thr Met
705 710 715 720
His Arg Lys Lys
<210> 112
<211> 743
<212> PRT
<213> 琴叶拟南芥
<400> 112
Met Val Trp Asn Leu Ala Thr Leu Gly Leu Leu Leu Ser Phe Cys Leu
1 5 10 15
Glu Ser Leu Phe Gln Phe Val Phe Phe Leu Ser Gly Ile Leu Gln Gly
20 25 30
Asp Leu Arg Arg Arg Asn Gly Asn Gly Gly Ser Ser Asn His Glu Arg
35 40 45
Asn Glu Gln Ile Leu Phe Pro Lys Pro Glu Thr Leu Asp Tyr Glu Leu
50 55 60
Pro Cys Asp Thr Ser Tyr Pro His Gln Ile Gly Asp Asn Ala Ala Ser
65 70 75 80
Ser Ser Gly Ser Asn Val Lys Ser Leu Leu Ile Glu Met Gly Phe Cys
85 90 95
Pro Thr Leu Val Gln Lys Ala Ile Asp Glu Asn Gly Glu Asp Asp Ile
100 105 110
Glu Phe Leu Leu Glu Ile Leu Thr Lys Ser Thr Val Thr Glu Gln Pro
115 120 125
Gly Thr Ser Phe His Gly Leu Met Glu Pro Lys Pro Glu Pro Asp Ile
130 135 140
Glu Tyr Glu Thr Asp Gly Arg Arg Ile Ala Leu Leu Thr Met Gln Phe
145 150 155 160
Pro Glu Asn Leu Val Asp Phe Ala Leu Asp Arg Leu Gly Lys Asp Ala
165 170 175
Pro Ile Asp Glu Met Val Asp Phe Ile Val Ala Ala Gln Leu Ala Glu
180 185 190
Lys Tyr Ala Glu Glu Ser Glu Asp Ser Leu Asp Gly Ala Glu Ile Asn
195 200 205
Glu Glu Asp Glu Asp Val Thr Pro Val Thr Ala Arg Gly Pro Glu Val
210 215 220
Pro Asn Glu Gln Leu Phe Glu Thr Met Asp Lys Thr Leu His Leu Leu
225 230 235 240
Glu Met Gly Phe Ser Asn Asp Glu Ile Ser Met Ala Ile Glu Lys Ile
245 250 255
Gly Thr Glu Gly Gln Ile Ser Val Leu Ala Glu Ser Ile Val Thr Gly
260 265 270
Glu Phe Pro Ala Val Cys His Asp Asp Leu Glu Asp Ile Glu Lys Lys
275 280 285
Val Ser Ala Ala Ala Pro Ala Ala Asn Arg Ala Cys Leu Ser Lys Ser
290 295 300
Trp Arg Phe Val Gly Val Gly Ala Gln Lys Glu Asp Gly Cys Gly Gly
305 310 315 320
Ser Ser Ser Gly Thr Ala Asn Ile Lys Pro Asp Pro Gly Ile Asp Ser
325 330 335
Phe Pro Phe Pro Ala Thr Ala Asn Val Gly Glu Thr Ser Lys Gly Lys
340 345 350
Arg Pro Lys Asp Glu Asp Glu Asn Ala Tyr Pro Glu Glu Tyr Thr Asp
355 360 365
His Asp Asp Arg Gly Lys Arg Leu Arg Pro Glu Tyr Met Gly Asp Ser
370 375 380
Ser Ser Phe Met Glu Thr Pro Trp Met Gln Asp Glu Trp Lys Asp Asn
385 390 395 400
Thr Tyr Glu Phe Pro Ser Val Met Gln Pro Arg Leu Ser Gln Ser Leu
405 410 415
Gly Pro Asn Val Ala Lys Arg Pro Tyr Phe Phe Tyr Gly Gln Leu Gly
420 425 430
Glu Leu Ser Pro Ser Trp Trp Ser Lys Ile Ser Gly Phe Leu Phe Gly
435 440 445
Ile His Pro Glu His Val Asp Thr Arg Leu Cys Ser Ala Leu His Arg
450 455 460
Thr Glu Gly Tyr Leu His Asn Leu Pro Thr Val Asn Arg Phe Asn Ile
465 470 475 480
Leu Pro Lys Pro Arg Leu Thr Ile Gln Asp Ala Met Pro Gln Met Lys
485 490 495
Ser Trp Trp Pro Gln Trp Asp Ile Arg Lys His Phe Asn Ser Gly Thr
500 505 510
Cys Ser Asn Arg Lys Asp Val Thr Leu Leu Cys Glu Arg Ile Gly Arg
515 520 525
Arg Ile Ala Glu Cys Arg Gly Lys Pro Thr Gln Gln Asp Gln Thr Leu
530 535 540
Ile Leu Arg His Cys His Thr Ser Asn Leu Ile Trp Ile Ala Pro Asn
545 550 555 560
Ile Leu Ser Pro Val Glu Pro Glu His Leu Glu Cys Ile Met Gly Tyr
565 570 575
Pro Thr Asn His Thr Asn Ile Gly Gly Gly Arg Leu Ala Glu Arg Leu
580 585 590
Lys Leu Phe Asp Tyr Cys Phe Gln Thr Asp Thr Leu Gly Tyr His Phe
595 600 605
Ser Val Leu Lys Ser Met Phe Pro Glu Gly Leu Thr Val Leu Ser Leu
610 615 620
Phe Ser Gly Ile Gly Gly Ala Glu Ile Ala Leu Asp Arg Leu Gly Ile
625 630 635 640
His Leu Lys Gly Val Val Ser Val Glu Ser Cys Gly Leu Ser Arg Asn
645 650 655
Ile Leu Lys Arg Trp Trp Gln Thr Ser Gly Gln Thr Gly Glu Leu Val
660 665 670
Gln Ile Glu Glu Ile Lys Ser Leu Thr Thr Lys Lys Leu Glu Thr Leu
675 680 685
Val Gln Arg Phe Gly Gly Phe Asp Phe Val Ile Cys Gln Asn Pro Ser
690 695 700
Thr Pro Leu Asp Leu Ser Lys Glu Ile Ser Asn Ser Glu Ala Cys Glu
705 710 715 720
Phe Asp Tyr Thr Leu Phe Asn Glu Phe Ala Arg Val Thr Lys Arg Val
725 730 735
Arg Asp Met Met Glu Leu Ser
740
<210> 113
<211> 626
<212> PRT
<213> 拟南芥
<400> 113
Met Val Ile Trp Asn Asn Asp Asp Asp Asp Phe Leu Glu Ile Asp Asn
1 5 10 15
Phe Gln Ser Ser Pro Arg Ser Ser Pro Ile His Ala Met Gln Cys Arg
20 25 30
Val Glu Asn Leu Ala Gly Val Ala Val Thr Thr Ser Ser Leu Ser Ser
35 40 45
Pro Thr Glu Thr Thr Asp Leu Val Gln Met Gly Phe Ser Asp Glu Val
50 55 60
Phe Ala Thr Leu Phe Asp Met Gly Phe Pro Val Glu Met Ile Ser Arg
65 70 75 80
Ala Ile Lys Glu Thr Gly Pro Asn Val Glu Thr Ser Val Ile Ile Asp
85 90 95
Thr Ile Ser Lys Tyr Ser Ser Asp Cys Glu Ala Gly Ser Ser Lys Ser
100 105 110
Lys Ala Ile Asp His Phe Leu Ala Met Gly Phe Asp Glu Glu Lys Val
115 120 125
Val Lys Ala Ile Gln Glu His Gly Glu Asp Asn Met Glu Ala Ile Ala
130 135 140
Asn Ala Leu Leu Ser Cys Pro Glu Ala Lys Lys Leu Pro Ala Ala Val
145 150 155 160
Glu Glu Glu Asp Gly Ile Asp Trp Ser Ser Ser Asp Asp Asp Thr Asn
165 170 175
Tyr Thr Asp Met Leu Asn Ser Asp Asp Glu Lys Asp Pro Asn Ser Asn
180 185 190
Glu Asn Gly Ser Lys Ile Arg Ser Leu Val Lys Met Gly Phe Ser Glu
195 200 205
Leu Glu Ala Ser Leu Ala Val Glu Arg Cys Gly Glu Asn Val Asp Ile
210 215 220
Ala Glu Leu Thr Asp Phe Leu Cys Ala Ala Gln Met Ala Arg Glu Phe
225 230 235 240
Ser Glu Phe Tyr Thr Glu His Glu Glu Gln Lys Pro Arg His Asn Ile
245 250 255
Lys Lys Arg Arg Phe Glu Ser Lys Gly Glu Pro Arg Ser Ser Val Asp
260 265 270
Asp Glu Pro Ile Arg Leu Pro Asn Pro Met Ile Gly Phe Gly Val Pro
275 280 285
Asn Glu Pro Gly Leu Ile Thr His Arg Ser Leu Pro Glu Leu Ala Arg
290 295 300
Gly Pro Pro Phe Phe Tyr Tyr Glu Asn Val Ala Leu Thr Pro Lys Gly
305 310 315 320
Val Trp Glu Thr Ile Ser Arg His Leu Phe Glu Ile Pro Pro Glu Phe
325 330 335
Val Asp Ser Lys Tyr Phe Cys Val Ala Ala Arg Lys Arg Gly Tyr Ile
340 345 350
His Asn Leu Pro Ile Asn Asn Arg Phe Gln Ile Gln Pro Pro Pro Lys
355 360 365
Tyr Thr Ile His Asp Ala Phe Pro Leu Ser Lys Arg Trp Trp Pro Glu
370 375 380
Trp Asp Lys Arg Thr Lys Leu Asn Cys Ile Leu Thr Cys Thr Gly Ser
385 390 395 400
Ala Gln Leu Thr Asn Arg Ile Arg Val Ala Leu Glu Pro Tyr Asn Glu
405 410 415
Glu Pro Glu Pro Pro Lys His Val Gln Arg Tyr Val Ile Asp Gln Cys
420 425 430
Lys Lys Trp Asn Leu Val Trp Val Gly Lys Asn Lys Ala Ala Pro Leu
435 440 445
Glu Pro Asp Glu Met Glu Ser Ile Leu Gly Phe Pro Lys Asn His Thr
450 455 460
Arg Gly Gly Gly Met Ser Arg Thr Glu Arg Phe Lys Ser Leu Gly Asn
465 470 475 480
Ser Phe Gln Val Asp Thr Val Ala Tyr His Leu Ser Val Leu Lys Pro
485 490 495
Ile Phe Pro His Gly Ile Asn Val Leu Ser Leu Phe Thr Gly Ile Gly
500 505 510
Gly Gly Glu Val Ala Leu His Arg Leu Gln Ile Lys Met Lys Leu Val
515 520 525
Val Ser Val Glu Ile Ser Lys Val Asn Arg Asn Ile Leu Lys Asp Phe
530 535 540
Trp Glu Gln Thr Asn Gln Thr Gly Glu Leu Ile Glu Phe Ser Asp Ile
545 550 555 560
Gln His Leu Thr Asn Asp Thr Ile Glu Gly Leu Met Glu Lys Tyr Gly
565 570 575
Gly Phe Asp Leu Val Ile Gly Gly Ser Pro Cys Asn Asn Leu Ala Gly
580 585 590
Gly Asn Arg Val Ser Arg Val Gly Leu Glu Gly Asp Gln Ser Ser Leu
595 600 605
Phe Phe Glu Tyr Cys Arg Ile Leu Glu Val Val Arg Ala Arg Met Arg
610 615 620
Gly Ser
625
<210> 114
<211> 686
<212> PRT
<213> 蓖麻
<400> 114
Met Gly Val Ile Val Tyr Cys Phe Arg Ile Thr Thr Cys Lys Pro Val
1 5 10 15
Tyr Met Glu His Lys Thr Leu Tyr Leu Glu Ile Leu Gly Ile Ala Val
20 25 30
Ala Ser Lys Glu Met Asp Gly Asp Ser Phe Cys Gly Glu Gly Asp Asn
35 40 45
Phe Asp Trp Asp Ser Glu Asp Glu Arg Glu Ile Glu Asn Phe Gly Leu
50 55 60
Thr Asn Ser Ser Cys Leu Ala Val Pro Ala Val Glu Ala Thr Ala Arg
65 70 75 80
Ser Val Glu Lys Cys Leu Met Gly Phe Ser Cys Ile Leu Arg His Ala
85 90 95
Ser Ser Ser Val Arg Ser Ser Gly Thr Lys Val Met Asp His Phe Val
100 105 110
Gly Met Gly Phe Pro Glu Lys Met Val Ala Glu Ala Ile Gln Glu Asn
115 120 125
Gly Glu Glu Asn Ala Asp Leu Ile Leu Glu Thr Leu Leu Lys Phe Ser
130 135 140
Thr Ile Ser Ser Pro Ser Ser Ser Gly Ser Lys Leu Ile Asp His Phe
145 150 155 160
Val Gly Met Gly Phe Asp Val Glu Met Val Asp Arg Ala Ile Gln Glu
165 170 175
Asn Gly Glu Glu Lys Thr Asp Ser Ile Leu Glu Thr Leu Leu Thr Tyr
180 185 190
Ser Val Ile Glu Lys Ser His Gln Glu Gln Pro His Val Asp Ser Asp
195 200 205
His Trp Ser Ser Glu Tyr Glu Gly Ser Phe Leu Asn Asp Phe Ser Asp
210 215 220
Ile Asp Ser Ser Glu Asn Glu Glu Asn Gly Lys Ser Gln Pro Val Glu
225 230 235 240
Gly Asn Thr Leu Arg Phe Leu Ala Arg Met Gly Tyr Thr Thr Asp Glu
245 250 255
Ala Ser Ile Ala Leu Glu Arg Cys Gly Pro Asp Ala Asn Ile Ala Glu
260 265 270
Leu Thr Asp Phe Ile Cys Ala Ala Gln Met Ala Lys Ala Ala Asp Ala
275 280 285
Ser Phe Pro Glu Glu Lys Pro Lys Ile Arg His Phe Asp Asp Asp Tyr
290 295 300
Pro Lys Asn Lys Lys Arg Asn Tyr Ala Glu Tyr Asp Met Trp Lys Lys
305 310 315 320
Lys Lys Gln Met Lys Leu Asp Lys Lys Leu Leu Asn Gly Asp Asp Glu
325 330 335
Leu Ile Arg Leu Pro Asn Pro Met Leu Gly Phe Gly Val Pro Thr Asp
340 345 350
Pro Ala Ile Val Thr His Arg Lys Leu Pro Glu Ala Ala Leu Gly Pro
355 360 365
Pro Phe Phe Tyr Tyr Glu Asn Val Ala Leu Ala Pro Lys Gly Val Trp
370 375 380
Gln Thr Ile Ser Arg Phe Leu Tyr Asp Val Glu Pro Glu Phe Val Asp
385 390 395 400
Ser Lys Tyr Phe Cys Ala Ala Ala Arg Lys Arg Gly Tyr Val His Asn
405 410 415
Leu Pro Ile Gln Asn Arg Tyr Pro Leu Leu Pro Leu Pro Pro Asn Asn
420 425 430
Ile His Glu Ala Leu Pro Leu Thr Lys Arg Trp Trp Pro Ser Trp Asp
435 440 445
Thr Arg Thr Lys Leu Asn Cys Leu Gln Thr Cys Val Ala Ser Ala Lys
450 455 460
Leu Thr Asp Arg Ile Arg Lys Ala Leu Glu Asp Tyr Glu Gly Glu Pro
465 470 475 480
Pro Leu Ser Ile Arg Lys Tyr Val Leu Asp Glu Cys Arg Lys Trp Asn
485 490 495
Leu Leu Trp Val Gly Arg Asn Lys Leu Ala Pro Leu Glu Pro Asp Glu
500 505 510
Val Glu Met Leu Leu Gly Phe Pro Arg Asn His Thr Arg Gly Gly Gly
515 520 525
Ile Ser Arg Thr Asp Arg Tyr Lys Ser Leu Gly Asn Ser Phe Gln Val
530 535 540
Asp Thr Val Ala Tyr His Leu Ser Val Leu Lys Asp Met Phe Pro Glu
545 550 555 560
Gly Ile Ser Leu Leu Ser Leu Phe Ser Gly Ile Gly Gly Ala Glu Val
565 570 575
Ala Leu His Arg Leu Gly Ile Arg Leu Lys Arg Val Val Ser Val Glu
580 585 590
Ile Ser Glu Val Asn Arg Asn Ile Met Arg Cys Trp Trp Glu Gln Thr
595 600 605
Asn Gln Thr Gly Thr Leu Ile Asp Ile Ala Asp Val His Asp Leu Asn
610 615 620
Ala Asp Arg Leu Glu Gln Leu Met Ser Ser Phe Gly Gly Phe Asp Leu
625 630 635 640
Val Val Gly Gly Ser Pro Cys Asn Asn Leu Ala Gly Ser Asn Arg His
645 650 655
His Arg Asp Gly Leu Glu Gly Lys Glu Ser Ser Leu Phe Phe Asp Tyr
660 665 670
Cys Arg Ile Leu Asp Leu Val Lys Cys Ile Met Thr Arg Asn
675 680 685
<210> 115
<211> 590
<212> PRT
<213> 大豆
<400> 115
Met Gly Gly Asp Asp Ser Gly Leu Glu Ser Asp Asn Phe Asp Trp Asn
1 5 10 15
Thr Glu Asp Glu Leu Glu Ile Gln Asn Tyr Asn Ser Ser Ser Ser Cys
20 25 30
Leu Thr Leu Pro Asn Gly Asp Ala Val Thr Gly Ser Gly Glu Ala Ser
35 40 45
Ser Ser Ala Val Leu Ala Asn Ser Lys Val Leu Asp His Phe Val Ser
50 55 60
Met Gly Phe Ser Arg Glu Met Val Ser Lys Val Ile Gln Glu Tyr Gly
65 70 75 80
Glu Glu Asn Glu Asp Lys Leu Leu Glu Glu Leu Leu Thr Tyr Lys Ala
85 90 95
Leu Glu Ser Ser Ser Arg Pro Gln Gln Arg Ile Glu Pro Asp Pro Cys
100 105 110
Ser Ser Glu Asn Ala Gly Ser Ser Trp Asp Asp Phe Ser Asp Thr Asp
115 120 125
Ile Phe Ser Asp Asp Glu Glu Ile Ala Lys Thr Met Ser Glu Asn Asp
130 135 140
Asp Thr Leu Arg Ser Leu Val Lys Met Gly Tyr Lys Gln Val Glu Ala
145 150 155 160
Leu Ile Ala Ile Glu Arg Leu Gly Pro Asn Ala Ser Leu Glu Glu Leu
165 170 175
Val Asp Phe Ile Gly Val Ala Gln Met Ala Lys Ala Glu Asp Ala Leu
180 185 190
Leu Pro Pro Gln Glu Lys Leu Gln Tyr Asn Asp Tyr Ala Lys Ser Asn
195 200 205
Lys Arg Arg Leu Tyr Asp Tyr Glu Val Leu Gly Arg Lys Lys Pro Arg
210 215 220
Gly Cys Glu Lys Lys Ile Leu Asn Glu Asp Asp Glu Asp Ala Glu Ala
225 230 235 240
Leu His Leu Pro Asn Pro Met Ile Gly Phe Gly Val Pro Thr Glu Ser
245 250 255
Ser Phe Ile Thr His Arg Arg Leu Pro Glu Asp Ala Ile Gly Pro Pro
260 265 270
Tyr Phe Tyr Tyr Glu Asn Val Ala Leu Ala Pro Lys Gly Val Trp Gln
275 280 285
Thr Ile Ser Arg Phe Leu Tyr Asp Val Glu Pro Glu Phe Val Asp Ser
290 295 300
Lys Phe Phe Cys Ala Ala Ala Arg Lys Arg Gly Tyr Ile His Asn Leu
305 310 315 320
Pro Ile Gln Asn Arg Phe Pro Leu Leu Pro Leu Pro Pro Arg Thr Ile
325 330 335
His Glu Ala Phe Pro Leu Thr Lys Lys Trp Trp Pro Ser Trp Asp Ile
340 345 350
Arg Thr Lys Leu Asn Cys Leu Gln Thr Cys Ile Gly Ser Ala Lys Leu
355 360 365
Thr Glu Arg Ile Arg Lys Ala Val Glu Ile Tyr Asp Glu Asp Pro Pro
370 375 380
Glu Ser Val Gln Lys Tyr Val Leu His Gln Cys Arg Lys Trp Asn Leu
385 390 395 400
Val Trp Val Gly Arg Asn Lys Val Ala Pro Leu Glu Pro Asp Glu Val
405 410 415
Glu Thr Leu Leu Gly Phe Pro Arg Asn His Thr Arg Gly Gly Gly Ile
420 425 430
Ser Arg Thr Asp Arg Tyr Lys Ser Leu Gly Asn Ser Phe Gln Val Asp
435 440 445
Thr Val Ala Tyr His Leu Ser Val Leu Lys Glu Met Tyr Pro Asn Gly
450 455 460
Ile Asn Leu Leu Ser Leu Phe Ser Gly Ile Gly Gly Ala Glu Val Ala
465 470 475 480
Leu His Arg Leu Gly Ile Pro Leu Lys Asn Val Val Ser Val Glu Lys
485 490 495
Ser Glu Val Asn Arg Asn Ile Val Arg Ser Trp Trp Glu Gln Thr Asn
500 505 510
Gln Lys Gly Asn Leu Tyr Asp Met Asp Asp Val Arg Glu Leu Asp Gly
515 520 525
Asp Arg Leu Glu Gln Leu Met Ser Thr Phe Gly Gly Phe Asp Leu Ile
530 535 540
Val Gly Gly Ser Pro Cys Asn Asn Leu Ala Gly Ser Asn Arg Val Ser
545 550 555 560
Arg Asp Gly Leu Glu Gly Lys Glu Ser Ser Leu Phe Phe Asp Tyr Phe
565 570 575
Arg Ile Leu Asp Leu Val Lys Asn Met Ser Ala Lys Tyr Arg
580 585 590
<210> 116
<211> 603
<212> PRT
<213> 玉蜀黍
<400> 116
Met Val His Trp Val Ser Asp Ser Asp Gly Ser Asp Asn Phe Glu Trp
1 5 10 15
Asp Ser Asp Gly Asn Gly Glu Gln Thr Val Ser Phe Asn Ala Ala Gly
20 25 30
Ala Gly Ser Ser Ala Leu Ala Ala Thr Asn Thr Asp Ala Pro Gly Pro
35 40 45
Ser Thr Arg Val Ala Asn Gly Asn Gly Lys Ala Gly Arg Ser Ala Ser
50 55 60
Leu Val Gln Lys Tyr Val Asp Met Gly Phe Ser Glu Glu Ile Val Leu
65 70 75 80
Lys Ala Met Lys Asp Asn Gly Asp Asn Gly Ala Asp Ser Leu Val Glu
85 90 95
Leu Leu Leu Thr Tyr Gln Glu Leu Gly Asn Asp Leu Lys Val Asp Asn
100 105 110
Asp Phe Ala Ser Ser Cys Ala Pro Lys Thr Ala Asp Asp Ser Asp Asp
115 120 125
Asp Asp Thr Leu Glu Ile Trp Asp Asp Glu Asp Ala Gly Gly Arg Ser
130 135 140
Thr Arg Val Ala Asn Ser Val Asp Asp Ser Asp Asp Glu Asp Phe Leu
145 150 155 160
His Glu Met Ser Arg Lys Asp Glu Lys Val Asp Ser Leu Val Lys Met
165 170 175
Gly Phe Pro Glu Asp Glu Ala Ala Leu Ala Ile Thr Arg Cys Gly Pro
180 185 190
Asp Ala Ser Ile Ser Val Leu Val Asp Ser Ile Tyr Ala Ser Gln Thr
195 200 205
Ala Gly Asp Gly Tyr Cys Gly Asn Leu Ser Asp Tyr Glu Asp Asn Ser
210 215 220
Tyr Gly Gly Arg Ser Thr Gly Asn Lys Lys Lys Arg Lys Arg Tyr Gly
225 230 235 240
Gly Gln Ala Gln Gly Ser Arg Gly Pro Leu Asp Gly Ser Cys Asp Glu
245 250 255
Pro Met Pro Leu Pro His Pro Met Val Gly Phe Asn Leu Pro Asp Gln
260 265 270
Trp Ser Arg Arg Val Asp Arg Ser Leu Pro Ala Gln Ala Ile Gly Pro
275 280 285
Pro Tyr Phe Tyr Tyr Glu Asn Val Ala Leu Ala Pro Lys Gly Val Trp
290 295 300
Thr Thr Ile Ser Arg Phe Leu Tyr Asp Ile Gln Pro Glu Phe Val Asp
305 310 315 320
Ser Lys Tyr Phe Cys Ala Ala Ala Arg Lys Arg Gly Tyr Ile His Asn
325 330 335
Leu Pro Leu Glu Asn Arg Ser Pro Leu Leu Pro Ile Pro Pro Lys Thr
340 345 350
Ile Ser Glu Ala Phe Pro Arg Thr Lys Arg Trp Trp Pro Ser Trp Asp
355 360 365
Pro Arg Arg Gln Phe Asn Cys Leu Gln Thr Cys Val Ser Ser Ala Lys
370 375 380
Leu Leu Glu Arg Ile Arg Val Ala Leu Thr Asn Ser Ser Asp Pro Pro
385 390 395 400
Pro Pro Arg Val Gln Lys Tyr Val Leu Glu Glu Cys Arg Lys Trp Asn
405 410 415
Leu Ala Trp Val Gly Leu Asn Lys Val Ala Pro Leu Glu Pro Asp Glu
420 425 430
Met Glu Phe Leu Leu Gly Phe Pro Lys Asp His Thr Arg Gly Ile Ser
435 440 445
Arg Thr Glu Arg Tyr Arg Ser Leu Gly Asn Ser Phe Gln Val Asp Thr
450 455 460
Val Ala Tyr His Leu Ser Val Leu Lys Asp Leu Phe Pro Gln Gly Met
465 470 475 480
Asn Val Leu Ser Leu Phe Ser Gly Ile Gly Gly Ala Glu Val Ala Leu
485 490 495
His Arg Leu Gly Ile Arg Met Asn Thr Val Ile Ser Val Glu Lys Ser
500 505 510
Glu Val Asn Arg Thr Ile Leu Lys Ser Trp Trp Asp Gln Thr Gln Thr
515 520 525
Gly Thr Leu Ile Glu Ile Thr Asp Val Gln Thr Leu Ser Ser Glu Arg
530 535 540
Ile Glu Ala Tyr Ile Arg Arg Ile Gly Gly Phe Asp Leu Val Ile Gly
545 550 555 560
Gly Ser Pro Cys Asn Asn Leu Thr Gly Ser Asn Arg His His Arg Asp
565 570 575
Gly Leu Glu Gly Glu His Ser Ala Leu Phe His His Tyr Phe Arg Ile
580 585 590
Leu His Ala Val Lys Ser Ile Met Glu Arg Leu
595 600
<210> 117
<211> 588
<212> PRT
<213> 蒺藜苜蓿
<400> 117
Met Gly Asp Tyr Ser Gly Leu Asp Ser Asp Ile Asp Trp Asn Thr Asp
1 5 10 15
Asp Glu Leu Glu Ile Glu Ser Phe Gln Pro Ser Cys Ser Thr Val Val
20 25 30
Pro Ser Gly Gln Thr Ile Thr Ala Gly Ser Val Glu Pro Ser Ser Phe
35 40 45
Ala Gly Pro Ser Asn Thr Lys Val Phe Asp His Phe Ile Ser Met Gly
50 55 60
Phe Pro Gly Glu Val Val Ser Lys Val Ile Gln Glu His Gly Glu Glu
65 70 75 80
Asn Glu Glu Lys Leu Leu Asn Glu Ile Leu Thr Tyr Ser Val Leu Glu
85 90 95
Ser Ser Pro Gln Gln Gln Gln Pro Ala Glu Leu Asp Pro Thr Ser Ser
100 105 110
Glu Cys Ala Gly Ser Ser Trp Glu Asp Leu Ser Glu Asp Asp Phe Phe
115 120 125
Ser Asp Glu Glu Leu Pro Lys Phe Asp Ser Thr Asn Asp Asp Thr Leu
130 135 140
Thr Lys Leu Val Lys Met Gly Phe Glu Glu Glu Glu Ala Leu Val Ala
145 150 155 160
Ile Asp Arg Ile Gly Ser Asp Ser Leu Glu Ala Leu Val Asp Phe Ile
165 170 175
Gly Ala Ala Gln Val Ala Lys Ala Glu Asn Ala Leu Leu Leu Pro Glu
180 185 190
Asp Lys Pro Gly Cys Ser Gly Asn Pro Lys Leu Lys Lys Arg Ser Leu
195 200 205
Tyr Glu Tyr Glu Val Leu Gly Lys Lys Arg Pro Lys Leu Glu Lys Arg
210 215 220
Thr Leu Cys Glu Asp Glu Glu Glu Ala Gln Thr Leu Asn Leu Pro Asn
225 230 235 240
Pro Met Met Gly Phe Gly Ile Pro Asn Glu Pro Ser Ser Met Ile Thr
245 250 255
His Arg Arg Leu Pro Glu Asn Ala Val Gly Pro Pro Tyr Phe Tyr Tyr
260 265 270
Glu Asn Val Ala Leu Ala Pro Lys Gly Val Trp Gln Thr Ile Ser Arg
275 280 285
Phe Leu Tyr Asp Val Glu Pro Glu Tyr Val Asp Ser Lys Tyr Phe Cys
290 295 300
Ala Ala Ala Arg Lys Arg Gly Tyr Ile His Asn Leu Pro Ile Val Asn
305 310 315 320
Arg Phe Pro Leu Leu Pro Leu Pro Pro Arg Thr Ile His Asp Ala Phe
325 330 335
Pro Leu Leu Arg Arg Trp Trp Pro Thr Trp Asp Pro Arg Thr Lys Leu
340 345 350
Asn Cys Leu Gln Thr Val His Ala Ser Ala Lys Leu Thr Asp Arg Ile
355 360 365
Arg Lys Ala Val Glu Ser Cys Asp Asp Phe Glu Glu Pro Ser Glu Thr
370 375 380
Val Lys Lys Tyr Val Leu Asp Gln Cys Arg Lys Trp Asn Leu Val Trp
385 390 395 400
Val Gly Lys Asn Lys Val Ala Pro Leu Glu Pro Asp Glu Val Glu Met
405 410 415
Leu Leu Gly Phe Pro Arg Asn His Thr Arg Gly Gly Gly Ile Ser Arg
420 425 430
Thr Asp Arg Phe Lys Ser Leu Gly Asn Ser Phe Gln Val Asp Thr Val
435 440 445
Ala Tyr His Leu Ser Val Leu Lys Glu Met Tyr Pro Lys Gly Val Asn
450 455 460
Leu Leu Ser Leu Phe Ser Gly Ile Gly Gly Ala Glu Val Ala Leu His
465 470 475 480
Arg Leu Gly Ile Pro Leu Arg Asn Val Val Ser Val Glu Lys Ser Glu
485 490 495
Val Asn Arg Asn Ile Val Arg Ser Trp Trp Glu Gln Thr Asn Gln Arg
500 505 510
Gly Asn Leu Val Asp Phe Asp Asp Val Gln Gln Leu Asp Ala Asp Arg
515 520 525
Leu Glu Arg Leu Met Gly Ala Phe Gly Gly Phe Asp Leu Ile Val Gly
530 535 540
Gly Ser Pro Cys Asn Asn Leu Ala Gly Ser Asn Arg Val Ser Arg Asn
545 550 555 560
Gly Leu Glu Gly Ser Glu Ser Ile Leu Phe Tyr Glu Tyr Phe Arg Ile
565 570 575
Leu Asp Leu Val Lys Val Met Ala Pro Arg Phe Arg
580 585
<210> 118
<211> 740
<212> PRT
<213> 两色蜀黍
<400> 118
Met Pro Val Ala Tyr His Gln Arg Pro Ala His Cys Trp Cys Cys Leu
1 5 10 15
Ala Gly Ala Ala Arg Glu Phe Leu Val Glu Thr Cys Leu Glu Ile Arg
20 25 30
Arg Leu Pro Leu Ser Ser Lys Ser Gly Gln Ser Arg Asp Gly Gly Gly
35 40 45
Arg Gln Ser Arg Pro Val Ala Gly Ala Ala Ser Cys Ser Ser Ser Ser
50 55 60
Ser Ala Thr Ala Ser Gln Leu Pro Ala Arg Arg Gly Arg Arg Arg Arg
65 70 75 80
Glu Gly Arg Ser Gly Arg Gly Ser Pro Arg Arg Gly Gly Ala Asp Gly
85 90 95
Asp Gly Arg Gly Glu Ala Ala Glu Val Val Arg Gly Gly Ala Glu Arg
100 105 110
Gly Cys Ala Val Pro Leu Arg Pro Val Pro Gln Pro Pro Glu Pro Leu
115 120 125
Arg Glu Gly Ser Gly Met Ala His Trp Val Ser Asp Gly Asp Gly Ser
130 135 140
Asp Ser Phe Glu Trp Asp Ser Asp Gly Asn Gly Glu Glu Ala Gly Ser
145 150 155 160
Phe Asn Thr Ala Gly Ala Ser Ser Ser Ala Met Ala Ser Thr Asn Thr
165 170 175
Asp Ala Pro Gly Pro Ser Arg Arg Val Ser Gln Val Ala Asn Gly Asn
180 185 190
Gly Lys Ala Gly Pro Ser Ala Ser Leu Val Gln Arg Tyr Ile Asp Met
195 200 205
Gly Phe Ala Glu Glu Ile Val Val Lys Ala Ile Lys Asp Asn Gly Asp
210 215 220
Asn Gly Ala Asp Ala Leu Val Glu Leu Leu Leu Thr Tyr Gln Glu Leu
225 230 235 240
Gly Asn Asp Leu Asn Val Asp Asn Gly Phe Ala Ser Gly Cys Val Pro
245 250 255
Gln Thr Val Asp Asp Ser Gly Asp Asp Asp Phe Leu Glu Asn Trp Asp
260 265 270
Asp Leu Asp Ala Gly Gly Arg Ser Thr Arg Val Ala Asn Ser Val Asp
275 280 285
Asp Ser Gly Asp Glu Asp Phe Leu His Glu Met Ser Gln Lys Asp Asn
290 295 300
Lys Ile Asp Ser Leu Val Lys Met Gly Phe Pro Glu Asp Glu Ala Ala
305 310 315 320
Leu Ala Ile Thr Arg Cys Gly Gln Asp Ala Ser Ile Ser Val Leu Ala
325 330 335
Asp Ser Ile Tyr Ala Ser Gln Thr Ala Gly Asp Gly Tyr Cys Gly Asn
340 345 350
Leu Ser Asp Tyr Glu Asp Gly Gly Arg Asn Lys Gly Arg Phe Met Asp
355 360 365
Gly Asn Asn Lys Lys Arg Lys Arg Tyr Gly Ser Gln Ala Leu Gly Ser
370 375 380
Arg Gly Pro Leu Asp Gly Ser Ala Asp Glu Pro Met Leu Leu Pro Asn
385 390 395 400
Pro Met Val Gly Phe Ser Leu Pro Asp Gln Trp Pro Arg Pro Val Asn
405 410 415
Arg Asp Leu Pro Ala Leu Ala Met Gly Pro Pro Tyr Phe Tyr Tyr Glu
420 425 430
Asn Val Ala Leu Ala Pro Lys Gly Val Trp Thr Ile Ile Ser Arg Phe
435 440 445
Leu Tyr Asp Ile Gln Pro Glu Phe Val Asp Ser Lys Tyr Phe Cys Ala
450 455 460
Ala Ala Arg Lys Arg Gly Tyr Ile His Asn Leu Pro Leu Glu Asn Arg
465 470 475 480
Ser Pro Leu Leu Pro Lys Pro Pro Arg Thr Ile Thr Val Ala Phe Pro
485 490 495
His Thr Lys Arg Trp Trp Pro Ser Trp Asp Pro Arg Gln Gln Phe Asn
500 505 510
Cys Leu Gln Thr Cys Val Ser Ser Ala Lys Leu Leu Glu Lys Ile Arg
515 520 525
Val Thr Leu Thr Asn Ser Ser Asp Pro Pro Pro Pro Arg Val Gln Lys
530 535 540
Leu Val Leu Glu Glu Cys Arg Lys Trp Asn Leu Ala Trp Val Gly Leu
545 550 555 560
Asn Lys Val Ala Pro Leu Glu Pro Asp Glu Met Glu Phe Leu Leu Gly
565 570 575
Phe Pro Lys Asp His Thr Arg Gly Ile Cys Arg Thr Glu Arg Phe Arg
580 585 590
Ser Leu Gly Asn Ser Phe Gln Val Asp Thr Val Ala Tyr His Leu Ser
595 600 605
Val Leu Lys Asp Met Tyr Pro Glu Gly Met Asn Val Leu Ser Leu Phe
610 615 620
Ser Gly Ile Gly Gly Ala Glu Val Ala Leu His Arg Leu Gly Ile Arg
625 630 635 640
Met Lys Thr Val Ile Ser Val Glu Lys Ser Glu Val Asn Arg Thr Ile
645 650 655
Leu Arg Ser Trp Trp Asp Gln Thr Gln Thr Gly Thr Leu Ile Glu Ile
660 665 670
Asn Asp Val Gln Thr Leu Thr Ser Glu Arg Ile Glu Ala Tyr Ile Arg
675 680 685
Arg Phe Gly Gly Phe Asp Leu Val Ile Gly Gly Ser Pro Cys Asn Asn
690 695 700
Leu Ala Gly Ser Asn Arg His His Arg Asn Gly Leu Glu Gly Glu His
705 710 715 720
Ser Ser Leu Phe Phe Gln Tyr Val Arg Ile Leu Glu Ser Val Lys Ser
725 730 735
Ile Gln Arg Leu
740
<210> 119
<211> 598
<212> PRT
<213> 稻
<400> 119
Met Val Asp Trp Ala Ser Asp Ser Asp Asn Asp Lys Phe Glu Trp Asp
1 5 10 15
Thr Asp Gly Glu Ala Glu Thr Ser Ser Ala Pro Ala Leu Arg Asn Ile
20 25 30
Asp Ala Pro Gly Pro Ser Thr Arg Leu Pro Gln Asp Ala Asn Gly Lys
35 40 45
Ala Asn Gly Ser Gly Ala Leu Val Ala Glu Phe Met Gly Met Gly Phe
50 55 60
Pro Lys Glu Met Ile Leu Lys Ala Ile Lys Glu Ile Gly Asp Thr Asp
65 70 75 80
Thr Glu Gln Leu Leu Glu Leu Leu Leu Thr Tyr Gln Ala Ile Gly Gly
85 90 95
Asp Ala Ser Val Gly Asn Cys Ser Ala Ser Ala Cys Ala Pro Gln Thr
100 105 110
Leu Glu Val Asp Glu Glu Glu Asp Asp Thr Asn Trp Asp Glu Tyr Asp
115 120 125
Thr Ala Gly Asn Cys Asp Arg Thr Pro His Ser Asp Gly Ser Gly Asp
130 135 140
Glu Asp Phe Phe Gln Glu Met Ser Glu Lys Asp Glu Lys Met Lys Ser
145 150 155 160
Leu Val Asn Met Gly Phe Pro Glu Asp Glu Ala Lys Met Ala Ile Asp
165 170 175
Arg Cys Gly Leu Asp Ala Pro Val Ala Val Leu Val Asp Ser Ile Tyr
180 185 190
Ala Ser Gln Glu Ala Gly Asn Gly Tyr Ser Ala Asn Leu Ser Asp Tyr
195 200 205
Glu Asp Thr Glu Phe Ser Ser Phe Gly Gly Arg Lys Lys Thr Arg Phe
210 215 220
Val Asp Gly Ser Lys Lys Arg Lys Arg Tyr Gly Ser Gly Pro Ser Gly
225 230 235 240
Asn Gln Val Pro Phe Asp Gly Ser His Glu Glu Pro Met Pro Leu Pro
245 250 255
Asn Pro Met Val Gly Phe Ser Leu Pro Asn Glu Arg Leu Arg Ser Val
260 265 270
His Arg Asn Leu Pro Asp Gln Ala Leu Gly Pro Pro Phe Phe Tyr Tyr
275 280 285
Glu Asn Val Ala Leu Ala Pro Lys Gly Val Trp Thr Thr Ile Ser Arg
290 295 300
Phe Leu Tyr Asp Ile Gln Pro Glu Phe Val Asp Ser Lys Tyr Phe Cys
305 310 315 320
Ala Ala Ala Arg Lys Arg Gly Tyr Ile His Asn Leu Pro Ile Glu Asn
325 330 335
Arg Ser Pro Val Leu Pro Met Pro Pro Lys Thr Ile Ser Glu Ala Phe
340 345 350
Pro Asn Thr Lys Arg Trp Trp Pro Ser Trp Asp Pro Arg Arg Gln Phe
355 360 365
Asn Cys Leu Gln Thr Cys Met Ala Ser Ala Lys Leu Thr Glu Arg Ile
370 375 380
Arg Cys Ala Leu Gly Arg Phe Ser Asp Val Pro Thr Pro Gln Val Gln
385 390 395 400
Lys Tyr Val Leu Asp Glu Cys Arg Lys Trp Asn Leu Val Trp Val Gly
405 410 415
Lys Asn Lys Val Ala Pro Leu Glu Pro Asp Glu Met Glu Phe Leu Leu
420 425 430
Gly Tyr Pro Arg Asn His Thr Arg Gly Val Ser Arg Thr Glu Arg Tyr
435 440 445
Arg Ala Leu Gly Asn Ser Phe Gln Val Asp Thr Val Ala Tyr His Leu
450 455 460
Ser Val Leu Arg Asp Leu Phe Pro Asn Gly Met Asn Val Leu Ser Leu
465 470 475 480
Phe Ser Gly Ile Gly Gly Ala Glu Val Ala Leu His Arg Leu Gly Ile
485 490 495
His Met Lys Thr Val Ile Ser Val Glu Lys Ser Glu Val Asn Arg Thr
500 505 510
Ile Leu Lys Ser Trp Trp Asp Gln Thr Gln Thr Gly Thr Leu Ile Glu
515 520 525
Ile Ala Asp Val Arg His Leu Thr Thr Glu Arg Ile Glu Thr Phe Ile
530 535 540
Arg Arg Phe Gly Gly Phe Asp Leu Val Ile Gly Gly Ser Pro Cys Asn
545 550 555 560
Asn Leu Ala Gly Ser Asn Arg His His Arg Asp Gly Leu Glu Gly Glu
565 570 575
His Ser Ala Leu Phe Tyr Asp Tyr Ile Arg Ile Leu Glu His Val Lys
580 585 590
Ala Thr Met Ser Ala Val
595
<210> 120
<211> 592
<212> PRT
<213> 二穗短柄草
<400> 120
Met Pro Gln Asp Cys Ile Ser Asp Ser Asp Asp Ser Ala Lys Phe Glu
1 5 10 15
Trp Glu Ser Asp Asn Glu Ala Glu Pro Ser Ser Ala Pro Val Leu Arg
20 25 30
Asn Phe Asp Ala Pro Gly Pro Ser Thr Val Trp Gln Asp Ala Asn Gly
35 40 45
Trp Ala Asn Gly Asp Ala Pro Ser Thr Ser Leu Val Glu Glu Tyr Val
50 55 60
Gly Met Gly Phe Pro Lys Glu Met Val Leu Lys Ala Ile Lys Glu Ile
65 70 75 80
Gly His Asn Asp Ala Asn Ala Leu Leu Glu Leu Leu Leu Thr Tyr Lys
85 90 95
Val Leu Gly Glu Asp Pro Thr Val Gly Asn Cys Ser Thr Leu Gly Cys
100 105 110
Ala Pro Gln Ser Val Glu Asp Asp Asp Asp Gly Asp Leu Asp Ser Glu
115 120 125
Asp Trp Asp Asp Glu Asp Asp Ala Asp Gly Gly Glu Pro Asn Phe Asp
130 135 140
Ser Ser Gly Asp Glu Asp Phe Leu Gln Glu Met Ser Glu His Asp Lys
145 150 155 160
Lys Ile Lys Ser Leu Val Asp Met Gly Phe Pro Glu Asp Glu Ser Asn
165 170 175
Met Ala Ile Val Arg Cys Gly Val Asp Ala Ala Leu Thr Val Leu Val
180 185 190
Asp Ser Ile Tyr Ala Ser Gln Ala Ala Gly Asp Cys Asn Ser Arg Asn
195 200 205
Ser Ser His His Glu Val Cys Asp Ser Phe Gly Gly Arg Arg Asn Lys
210 215 220
Lys Lys Arg Lys Gln Tyr Gly Gly Gly Ala Gln Gly Asn Arg Pro Ser
225 230 235 240
Glu Cys His Glu Glu Leu Met Pro Leu Pro Asn Pro Met Val Gly Phe
245 250 255
Ser Leu Pro Thr Ala Arg Leu Pro Ser Val Ser Arg Arg Leu Pro Lys
260 265 270
Gln Ala Thr Gly Pro Pro Phe Phe Tyr Tyr Glu Asn Val Ala Leu Ala
275 280 285
Pro Lys Gly Val Trp Thr Ile Ile Ser Arg Asn Leu Tyr Asp Ile Ala
290 295 300
Pro Glu Phe Val Asp Ser Lys Tyr Met Cys Ala Thr Ala Arg Lys Arg
305 310 315 320
Gly Tyr Val His Asn Leu Pro Ile Glu Asn Arg Ser Pro Leu Leu Pro
325 330 335
Leu Pro Pro Lys Thr Ile Phe Glu Ala Phe Pro His Tyr Lys Lys Trp
340 345 350
Trp Pro Ser Trp Asp Pro Arg Arg Gln Leu Asn Cys Val Gln Thr Cys
355 360 365
Val Ser Ser Ala Lys Leu Thr Glu Arg Ile Gln Cys Ala Leu Ala Arg
370 375 380
Ser Gly Asp Pro Pro Pro Leu His Val Gln Lys Tyr Val Met His Glu
385 390 395 400
Cys Arg Lys Trp Asn Leu Val Trp Val Gly Lys Asn Lys Val Ala Pro
405 410 415
Leu Glu Pro Asp Glu Met Glu Tyr Leu Leu Gly Tyr Pro Arg Asp His
420 425 430
Thr Arg Gly Ile Gly Lys Thr Ala Arg Tyr Lys Cys Leu Gly Asn Ser
435 440 445
Phe Gln Val Asp Thr Val Ala Tyr His Leu Ser Val Leu Arg Asp Ile
450 455 460
Phe Pro Asn Gly Leu Asn Val Leu Ser Leu Phe Thr Gly Ile Gly Gly
465 470 475 480
Gly Glu Val Ala Leu His Arg Leu Gly Ile His Met Lys Thr Val Val
485 490 495
Ser Val Glu Ile Ser Glu Val Asn Arg Arg Ile Leu Arg Gly Trp Trp
500 505 510
Asp Gln Thr Gln Thr Gly Thr Leu Ile Glu Ile Pro Asp Val Gln Ser
515 520 525
Phe Thr Ser Asp Lys Ile Arg Ser Phe Ile Arg Arg Phe Gly Gly Phe
530 535 540
Asp Leu Ile Ile Gly Gly Ser Pro Cys Asn Asn Leu Ala Gly Ser Asn
545 550 555 560
Arg His His Arg Asp Gly Leu Glu Gly Glu His Ser Ser Leu Phe Tyr
565 570 575
His Tyr Pro Arg Ile Leu Asp Thr Val Lys Asp Val Met Ala Gly Met
580 585 590
<210> 121
<211> 634
<212> PRT
<213> 毛果杨
<400> 121
Met Asp Gly Asp Ser Leu Cys Gly Asp Asn Asp Asp Phe Asp Trp Asp
1 5 10 15
Ser Glu Asp Glu Lys Glu Ile Glu Asn Phe Ala Ser Ser Ser Ser Ser
20 25 30
Ser Leu Arg Leu Pro Gln Val Glu Thr Arg Ser Ser Ser Ala Glu Ala
35 40 45
Ser Ser Ser Val Gly Ser Ser Ser Gly Ser Lys Met Ile Asp His Phe
50 55 60
Val Lys Met Gly Phe Pro Glu Lys Met Val Ala Glu Ala Ile Gln Glu
65 70 75 80
Asn Cys Lys Gly Glu Glu Asp Glu Asp Ser Ile Leu Glu Thr Leu Leu
85 90 95
Lys Tyr Ser Thr Ser Ser Ser Ala Ser Ser Ser Gly Ser Lys Leu Phe
100 105 110
Asp Arg Phe Val Gly Met Gly Phe Ala Glu Lys Met Val Ala Lys Ala
115 120 125
Ile Lys Glu Asn Gly Glu Gly Asp Ala Asp Ser Val Leu Glu Thr Leu
130 135 140
Leu Thr Tyr Ala Ala Ile Gly Lys Ser Pro Gln Glu Gln Pro Asn Asn
145 150 155 160
Asp Ser Asp His Cys Ser Ser Gly His Glu Gly Ser Phe Leu Asp Asp
165 170 175
Phe Ser Asp Val Asp Ser Ala Asp Asp Glu Val Ile Thr Lys Thr Val
180 185 190
Ser Asp Glu Asp Asn Lys Leu Ala Phe Leu Arg Arg Met Gly Tyr Lys
195 200 205
Glu Ala Asp Ala Ser Ile Ala Ile Thr Arg Cys Gly Thr Glu Ala Thr
210 215 220
Ile Ser Glu Leu Ala Asp Phe Ile Cys Ala Ala Gln Ile Ala Lys Ala
225 230 235 240
Glu Asp Ala Phe Phe Ala Glu Asp Glu Lys Lys Pro Lys His Leu Asp
245 250 255
Lys Gln Lys Lys Arg Ser Phe Leu Glu Ser Asp Val Leu Glu Lys Lys
260 265 270
Arg Gln Lys Gly Leu Glu Asn Gly Asp Asp Glu Gly Val Arg Leu Pro
275 280 285
Asn Pro Met Val Gly Phe Gly Val Pro Thr Glu Pro Gly Ile Val Thr
290 295 300
Arg Arg Thr Leu Ser Glu Ala Ala Ile Gly Pro Pro Phe Phe Tyr Tyr
305 310 315 320
Glu Asn Val Ala Leu Ala Pro Lys Gly Val Trp Gln Thr Ile Ser Arg
325 330 335
Phe Leu Tyr Asp Val Glu Pro Glu Phe Val Asp Ser Lys His Phe Cys
340 345 350
Ala Ala Ala Arg Lys Arg Gly Tyr Val His Asn Leu Pro Ile His Asn
355 360 365
Arg Phe Pro Leu Leu Pro Leu Pro Pro Asn Thr Ile His Glu Ala Leu
370 375 380
Pro Leu Thr Arg Lys Trp Trp Pro Ala Trp Asp Glu Arg Thr Lys Leu
385 390 395 400
Asn Cys Leu Gln Thr Cys Ile Ala Ser Ala Lys Leu Thr Glu Arg Ile
405 410 415
Arg Lys Ala Leu Glu Ala Tyr Glu Gly Glu Pro Pro Leu His Val Gln
420 425 430
Lys Phe Ile Met Asp Glu Cys Arg Lys Trp Asn Leu Val Trp Val Gly
435 440 445
Arg Asn Lys Val Ala Pro Leu Glu Ala Asp Glu Val Glu Met Leu Leu
450 455 460
Gly Phe Pro Arg Asn His Thr Arg Gly Gly Gly Ile Ser Arg Thr Asp
465 470 475 480
Arg Tyr Lys Ser Leu Gly Asn Ser Phe Gln Val Asp Thr Val Ala Tyr
485 490 495
His Leu Ser Ser Leu Lys Asp Leu Phe Pro Gly Gly Ile Asn Val Leu
500 505 510
Ser Leu Phe Ser Gly Ile Gly Gly Ala Glu Val Ala Leu His Arg Leu
515 520 525
Gly Ile Arg Leu Lys Asn Val Val Ser Val Glu Ile Ser Asn Val Asn
530 535 540
Arg Ser Ile Met Ser Cys Trp Trp Glu Gln Thr Asn Gln Thr Gly Asn
545 550 555 560
Leu Ile His Ile Glu Asp Val Gln His Leu Thr Ala Asp Arg Leu Glu
565 570 575
Gln Leu Met Asn Met Tyr Gly Ser Phe Asp Leu Val Val Gly Gly Ser
580 585 590
Pro Cys Asn Asn Leu Ala Gly Ser Asn Arg His His Arg Asp Gly Leu
595 600 605
Glu Gly Lys Glu Ser Ser Leu Phe Phe Asp Tyr Cys Arg Ile Leu Asp
610 615 620
Val Val Lys Asn Leu Thr Ser Arg Tyr Ser
625 630
<210> 122
<211> 616
<212> PRT
<213> 葡萄
<400> 122
Met Val Asp Pro Leu Tyr Phe Arg Ser Gly Leu Gly His Ala Leu Asp
1 5 10 15
Gly Asn Ile Ser Gly Glu Glu Ser Asp Ser Ile Asp Trp Asn Thr Asp
20 25 30
Asp Glu Leu Glu Thr Gln Asn Leu Pro Pro Ser Ser Ser Ser Thr Leu
35 40 45
Ile Ile Pro Gly Gly Glu Ala Ser Ile Ala Ser Gly Glu Ala Ser Ser
50 55 60
Ser Thr Gly Pro Ser Asn Ser Lys Leu Ile Asn His Phe Met Gly Met
65 70 75 80
Gly Phe Ser Glu Lys Met Val Val Lys Ala Ile Glu Gln Asp Gly Glu
85 90 95
Gly Asn Thr Asp Ser Ile Leu Glu Tyr Leu Leu Thr Tyr Ser Ala Leu
100 105 110
Glu Asn Cys Pro Gln Glu Leu Glu Asn Ser Pro Gln Ala Glu Pro Gln
115 120 125
Val Val Ser Asp Asn Cys Ser Ser Asp Tyr Asp Glu Ser Phe Leu Asp
130 135 140
Asp Phe Ser Asp Thr Asp Ser Cys His Glu Asn Gln Glu Asn Ile Asn
145 150 155 160
Ser Leu Ser Asp Lys Glu Lys Lys Leu Leu Phe Leu Ala Asp Met Gly
165 170 175
Tyr Thr Val Glu Glu Ala Ser Ile Ala Met Asp Arg Cys Gly Pro Asp
180 185 190
Ser Ser Val Val Glu Leu Thr Asp Phe Ile Ser Ala Ala Gln Ile Ser
195 200 205
Lys Ala Ala Asp Ala Asp Leu Gly Glu Leu Ser Asp Glu Glu Lys Pro
210 215 220
Arg Pro Lys His Leu Tyr Lys Asp Tyr Asn Lys Gln Lys Lys Arg Lys
225 230 235 240
Phe Leu Asp Tyr Arg Leu Trp Glu Arg Lys Lys Gln Arg Gly Tyr Glu
245 250 255
Lys Lys Leu Arg Asn Glu Asp Asp Glu Pro Ile Arg Leu Pro Asn Pro
260 265 270
Met Val Gly Phe Gly Val Pro Ala Glu Pro Cys Pro Val Phe His Arg
275 280 285
Thr Leu Pro Glu Ala Ala Val Gly Pro Pro Tyr Phe Tyr Tyr Glu Asn
290 295 300
Val Ala Leu Ala Pro Lys Gly Val Trp Ser Thr Ile Ser Arg Phe Leu
305 310 315 320
Tyr Asp Val Glu Pro Glu Phe Val Asp Ser Lys Tyr Phe Cys Ala Thr
325 330 335
Ala Arg Lys Arg Gly Tyr Ile His Asn Leu Pro Ile Gln Asn Arg Phe
340 345 350
Pro Leu Leu Pro Leu Pro Pro Leu Thr Ile Tyr Glu Ala Leu Pro Leu
355 360 365
Thr Arg Lys Trp Trp Pro Ser Trp Asp Thr Arg Thr Lys Leu Asn Cys
370 375 380
Ile Gln Thr Cys Val Gly Ser Ala Lys Leu Thr Asp Arg Ile Arg Lys
385 390 395 400
Ala Leu Glu Glu Trp Asp Gly Val Pro Pro Met Ser Val Gln Lys Tyr
405 410 415
Val Leu Asp Glu Cys Arg Lys Trp Asn Leu Val Trp Val Gly Arg Asn
420 425 430
Lys Leu Ala Pro Leu Glu Pro Asp Glu Val Glu Met Leu Leu Gly Phe
435 440 445
Pro Arg Asn His Thr Arg Gly Gly Gly Ile Ser Arg Thr Asp Arg Tyr
450 455 460
Lys Ser Leu Gly Asn Ser Phe Gln Val Asp Thr Val Ala Tyr His Phe
465 470 475 480
Ser Val Leu Lys Asp Met Phe Pro Gly Gly Ile Thr Leu Leu Ser Leu
485 490 495
Phe Ser Gly Ile Gly Gly Ala Glu Ile Ala Leu His Arg Leu Gly Ile
500 505 510
Pro Leu Lys Asn Val Val Ala Val Glu Ile Ser Glu Thr Asn Arg Asn
515 520 525
Ile Leu Arg Ser Trp Trp Glu Gln Thr Asn Gln Arg Gly Thr Leu Val
530 535 540
Asp Ile Ala Asp Val Gln Gln Leu Asn Gly Asp Arg Leu Glu Gln Leu
545 550 555 560
Ile His Thr Phe Gly Gly Phe Asp Leu Ile Val Gly Gly Ser Pro Cys
565 570 575
Asn Asn Leu Ala Gly Ser Asn Arg Val Ser Arg Asp Gly Leu Glu Gly
580 585 590
Lys Glu Ser Ala Leu Phe Tyr Asp Tyr Phe Arg Ile Leu Asp Leu Val
595 600 605
Lys Cys Ile Met Gly Arg Asn Gln
610 615
<210> 123
<211> 586
<212> PRT
<213> 黄瓜
<400> 123
Met Ala Gly Asp Asp Phe Gly Val Glu Val Asp Asn Ile Asp Trp Asp
1 5 10 15
Thr Glu Asp Glu Leu Glu Ile Glu Asn Ile Pro Leu Gly Ser His Ala
20 25 30
Ser Leu Ala Phe Pro Gly Val Glu Ala Asn Met Ala Ser Ser Ser Ala
35 40 45
Gly Pro Ser Asn Ser Lys Thr Val Asp Tyr Phe Ile Gly Met Gly Phe
50 55 60
Ser Ala Lys Met Val Ala Lys Ala Ile Glu Asp Asn Gly Glu Glu Asn
65 70 75 80
Thr Asp Ser Ile Leu Glu Thr Leu Leu Thr Leu Ser Ala Leu Glu Asn
85 90 95
Ser Pro Pro Lys Gln Gln His Val Asp Thr Asp Asp Ser Phe Ser Asp
100 105 110
Tyr Glu Gly Ser Phe Leu Asp Asp Phe Ser Asp Leu Asp Ser Asp Tyr
115 120 125
Glu Asn Glu Ala Thr Ala Lys Pro Val Ser Asp Ala Asp Asn Lys Met
130 135 140
Thr Phe Leu Val Asp Met Gly Tyr Ser Glu Asp Glu Ala Tyr Ala Ala
145 150 155 160
Ile Glu Arg Cys Gly Val His Ser Ser Phe Ala Glu Leu Thr Asp Phe
165 170 175
Ile Ser Ala Ala Gln Ile Ser Lys Ala Ala Asp Val Leu Leu Gly Asp
180 185 190
Leu Pro Val Arg Pro Lys Pro Lys Asn Leu Asn Gly Cys Ser Lys Leu
195 200 205
Asn Lys Arg Arg His Tyr Asp Asp Asp Thr Trp Lys Lys Lys Lys Pro
210 215 220
Arg Cys Phe Glu Asp Glu Asp Asp Asp Thr Ile Arg Leu Pro Asn Pro
225 230 235 240
Met Ile Gly Phe Gly Val Pro Asn Glu Leu Cys Leu Thr Val His Arg
245 250 255
Lys Ile Pro Glu Ala Ala Met Gly Pro Pro Tyr Phe Tyr Tyr Glu Asn
260 265 270
Val Ala Leu Ala Pro Lys Gly Val Trp Asn Thr Ile Ser Arg Phe Leu
275 280 285
Tyr Asp Val Glu Pro Glu Phe Val Asp Ser Lys Tyr Phe Cys Ala Ala
290 295 300
Ala Arg Lys Arg Gly Tyr Val His Asn Leu Pro Ile His Asn Arg Phe
305 310 315 320
Pro Leu Leu Pro Leu Pro Pro His Thr Val His Glu Ala Leu Pro Leu
325 330 335
Thr Arg Arg Trp Trp Pro Ser Trp Asp Thr Arg Thr Gln Leu Asn Cys
340 345 350
Leu Gln Thr Cys Ile Gly Ser Ala Arg Leu Thr Asp Arg Ile Arg Arg
355 360 365
Ala Leu Glu Asp Tyr Asp Gly Val Arg Asp Pro Pro Leu His Val Gln
370 375 380
Lys Phe Val Val Glu Gln Cys Arg Lys Trp Asn Leu Val Trp Val Gly
385 390 395 400
Lys Asn Lys Val Ala Pro Leu Glu Pro Asp Glu Val Glu Met Leu Leu
405 410 415
Gly Phe Pro Lys Asn His Thr Arg Gly Gly Gly Ile Ser Arg Thr Asp
420 425 430
Arg Tyr Lys Ser Leu Gly Asn Ser Phe Gln Val Asp Thr Val Ala Tyr
435 440 445
His Leu Ser Val Leu Lys Asp Met Phe Pro Gly Gly Ile Asn Leu Leu
450 455 460
Ser Leu Phe Ser Gly Ile Gly Gly Ala Glu Val Ala Leu His Arg Leu
465 470 475 480
Gly Ile Pro Leu Lys Asn Val Val Ser Val Asp Ile Ser Glu Val Asn
485 490 495
Arg Asn Ile Val Arg Cys Trp Trp Glu Gln Thr Asn Gln Lys Gly Thr
500 505 510
Leu Ile Asp Leu Ala Asp Val Gln Glu Leu Asp Ala Asp Arg Leu Gln
515 520 525
Tyr Tyr Met Asn Leu Phe Gly Gly Phe Asp Leu Val Val Gly Gly Ser
530 535 540
Pro Cys Asn Asn Leu Thr Gly Ser Asn Arg Tyr Thr Arg Asp Gly Leu
545 550 555 560
Glu Gly Lys Glu Ser Ile Leu Phe Tyr Asp Tyr Phe Arg Ile Leu Asp
565 570 575
Leu Val Lys Cys Ile Ala Thr Lys Thr Glu
580 585
<210> 124
<211> 628
<212> PRT
<213> 琴叶拟南芥
<400> 124
Met Ala Gln Asp Ser Ala Gly Asp Asp Asp Phe Val Asp Trp Asn Thr
1 5 10 15
Asp Asp Asp Leu Glu Ile Asp Asn Phe Gln Ser Ser Pro Pro Ser Ser
20 25 30
Pro Ile Gln Ser Arg Val Glu Thr Leu Val Gly Val Ala Val Thr Thr
35 40 45
Ser Ser Leu Ser Ser Pro Thr Glu Thr Thr Asp Leu Val Gln Met Gly
50 55 60
Phe Ser Asp Glu Ile Phe Ala Thr Leu Phe Asp Met Gly Phe Pro Val
65 70 75 80
Glu Met Ile Ala Arg Ala Ile Lys Glu Thr Gly Pro Asn Ala Glu Thr
85 90 95
Ser Val Ile Ile Asp Thr Ile Ser Lys Tyr Ser Ser Asn Cys Glu Ala
100 105 110
Gly Ser Ser Lys Ser Lys Ala Ile Asp His Phe Leu Ala Met Gly Phe
115 120 125
Asp Glu Glu Lys Val Ile Lys Ala Ile Gln Glu His Gly Glu Glu Asn
130 135 140
Met Glu Ala Ile Ala Asn Ala Leu Leu Ser Cys Ala Glu Ala Glu Lys
145 150 155 160
Leu Pro Val Val Glu Glu Glu Asp Val Ile Asp Trp Ser Ser Asp Asp
165 170 175
Glu Thr Asn Tyr Ser Asp Ile Leu Val Ser Asp Asp Glu Lys Asp Pro
180 185 190
Asn Ser Lys Glu Asn Ser Asn Gln Ile Arg Ser Leu Val Arg Met Gly
195 200 205
Phe Ser Glu Leu Glu Ala Ser Leu Ala Val Glu Arg Cys Gly Glu Asn
210 215 220
Val Asp Ile Ala Glu Leu Thr Asp Phe Leu Cys Ala Ala Lys Met Ala
225 230 235 240
Arg Glu Phe Ser Glu Phe His Thr Asp Pro Glu Glu Arg Lys Pro Arg
245 250 255
Asn Asp Ile Lys Lys Arg Arg Leu Glu Ser Lys Lys Glu Pro Arg Ser
260 265 270
Ser Val Asp Asp Glu Pro Val Arg Leu Pro Asn Pro Met Ile Gly Phe
275 280 285
Gly Val Pro Asn Glu Pro Gly Leu Ile Thr His Arg Ser Leu Pro Glu
290 295 300
Leu Ala Arg Gly Pro Pro Phe Phe Tyr Tyr Glu Asn Val Ala Leu Thr
305 310 315 320
Pro Lys Gly Val Trp Glu Thr Ile Ser Arg His Leu Phe Asp Ile Pro
325 330 335
Pro Glu Phe Val Asp Ser Lys Tyr Phe Cys Ala Ala Ala Arg Lys Arg
340 345 350
Gly Tyr Ile His Asn Leu Pro Ile Asn Asn Arg Phe Gln Ile Gln Pro
355 360 365
Pro Pro Lys Tyr Thr Ile His Asp Ala Phe Pro Leu Ser Lys Arg Trp
370 375 380
Trp Pro Asp Trp Asp Lys Arg Thr Lys Leu Asn Cys Ile Leu Thr Cys
385 390 395 400
Thr Gly Ser Ala Gln Leu Thr Asn Arg Ile Arg Leu Ala Leu Glu Pro
405 410 415
Tyr Asn Gly Glu Pro Glu Pro Pro Lys His Val Gln Arg Tyr Val Ile
420 425 430
Asp Gln Cys Arg Arg Trp Asn Leu Val Trp Val Gly Lys Asn Lys Ala
435 440 445
Ala Pro Leu Glu Pro Asp Glu Met Glu Asn Ile Leu Gly Phe Pro Lys
450 455 460
Asn His Thr Arg Gly Gly Gly Met Ser Arg Thr Glu Arg Tyr Lys Ser
465 470 475 480
Leu Gly Asn Ser Phe Gln Val Asp Thr Val Ala Tyr His Leu Ser Val
485 490 495
Leu Lys Pro Ile Phe Pro His Gly Ile Asn Val Leu Ser Leu Phe Thr
500 505 510
Gly Ile Gly Gly Gly Glu Val Ala Leu His Arg Leu Gln Ile Arg Met
515 520 525
Lys Leu Val Val Ser Val Glu Ile Ser Lys Val Asn Arg Asn Ile Leu
530 535 540
Lys Asp Phe Trp Glu Gln Thr Asn Gln Thr Gly Val Leu Ile Glu Phe
545 550 555 560
Ser Asp Ile Gln His Leu Thr Asn Asp Thr Ile Glu Gly Leu Met Glu
565 570 575
Lys Tyr Gly Gly Phe Asp Leu Val Ile Gly Gly Ser Pro Cys Asn Asn
580 585 590
Leu Ala Gly Gly Asn Arg Val Ser Arg Val Gly Leu Glu Gly Asp Gln
595 600 605
Ser Ser Leu Phe Phe Glu Tyr Cys Arg Ile Leu Glu Val Val Arg Ala
610 615 620
Met Arg Arg Ser
625
<210> 125
<211> 1047
<212> PRT
<213> 拟南芥
<400> 125
Met Asp Ser Ala Ile Glu Ile Ser Ser Gly Ser Asp Ser Asp Asp Glu
1 5 10 15
Val Pro Pro Gln Pro Val Trp Pro Gln Thr Arg Thr Arg Met Asp Pro
20 25 30
Thr Trp Leu Ser Arg Arg Pro Leu Pro Thr Val Asp Ser His Ala Arg
35 40 45
Ala Glu His Thr Asn Gln Ala Pro Pro Asn Gly Ala Ser Ser Asp Thr
50 55 60
Ser Arg Pro Gly Val Ser Lys Pro Phe Thr Gly Asn Gly Asn Thr Val
65 70 75 80
Asn Ser Arg Ile Ser Ser Gly Ser Gly Ala Asp Tyr Val Arg Leu Ser
85 90 95
Ser Glu Gln Ala Leu Lys Arg Thr Leu Pro Pro Ser Phe Asn Ser Pro
100 105 110
Pro Leu Pro Ala Arg Ser Gly Thr Asn Asn Ile Ser Asn Ala Ser Gly
115 120 125
Ser Arg Val Gly Val Asp Tyr Glu Arg Pro Leu Ser Gln Gln Ala Leu
130 135 140
Lys Arg Thr Leu Pro Pro Ser Phe Asn Pro Pro Pro Leu Pro Ser Arg
145 150 155 160
Ser Gly Thr Asn Asn Ile Arg Asn Ala Gly Gly Ser Arg Phe Gly Ala
165 170 175
Asp Tyr Ser His Pro Ala Val Ser Ala Val Gly Asn Lys Ser Thr Phe
180 185 190
Gly Asp His Tyr Ser Gly Ala His Ala Glu Ile Gly Ile Gln Arg Gly
195 200 205
Val Asn Gly Val Arg Ile Leu Pro Pro Ser Leu Thr His Gly Thr Ser
210 215 220
Ala Ser Val Leu His His Ala Gly Ser Ser Asp Pro Met His Arg Phe
225 230 235 240
Gly Gly Gly Glu Asp Arg Asn Pro Asp Asn Asp Glu Arg Leu Val Tyr
245 250 255
Gln Ala Ala Leu Gln Val Leu Asn Gln Pro Met Thr Glu Ser Asp Leu
260 265 270
Pro Pro Gly Thr Leu Ser Val Pro Leu Met Arg His Gln Lys Ile Ala
275 280 285
Leu Ala Trp Met Phe Gln Lys Glu Thr Ser Ser Phe Asn Cys Pro Gly
290 295 300
Gly Ile Leu Ala Asp Asp Gln Gly Leu Gly Lys Thr Val Ser Thr Ile
305 310 315 320
Ala Leu Ile Leu Lys Gln Lys Ile Val Ser Gln Leu Lys Ser Glu Ser
325 330 335
Ser Cys Lys Gln Glu Thr Glu Ala Leu Val Leu Asp Ala Asp Asp Glu
340 345 350
Ser Asp Asn Ala Lys His Glu Ser Gly Ser His Val Lys Pro Glu Leu
355 360 365
Lys Val Ser Ser Asn Ser Glu Thr Ser Val Leu Ser Ala Cys Gly Asn
370 375 380
Asp Glu Asn Asp Ser Ser Asp Met Glu Lys Ala Glu Asp Glu Glu Ala
385 390 395 400
Asn Ser Ser Thr Arg Ala Phe Gln Trp Lys Arg Pro Ala Ala Gly Thr
405 410 415
Leu Ile Val Cys Pro Ala Ser Val Val Arg Gln Trp Ala Arg Glu Leu
420 425 430
Asp Glu Lys Val Ser Glu Glu Ser Lys Leu Ser Val Leu Val Tyr His
435 440 445
Gly Ser Asn Arg Thr Lys Asp Pro Asn Glu Leu Ala Glu Tyr Asp Val
450 455 460
Val Val Thr Thr Tyr Ala Ile Val Thr Asn Glu Ala Pro Asn Lys Phe
465 470 475 480
Leu Val Asp Glu Asp Glu Asn Asp Glu Lys Asn Thr Asp Arg Tyr Gly
485 490 495
Leu Ala Ser Gly Phe Ser Asn Asn Lys Lys Arg Lys Val Val Val Gly
500 505 510
Ala Ser Lys Lys Ser Lys Arg Arg Gly Arg Lys Ser Thr Asn Asp Thr
515 520 525
Ser Ser Glu Pro Asp Cys Gly Pro Leu Gly Lys Val Gly Trp Phe Arg
530 535 540
Ile Val Leu Asp Glu Ala Gln Thr Ile Lys Asn Tyr Arg Thr Gln Met
545 550 555 560
Ala Arg Ser Cys Cys Thr Leu Arg Ala Lys Arg Arg Trp Cys Leu Ser
565 570 575
Gly Thr Pro Ile Gln Asn Thr Ile Asp Asp Leu Tyr Ser Tyr Phe Arg
580 585 590
Phe Leu Arg Tyr Asp Pro Tyr Ala Val Tyr Lys Ser Phe Tyr Ser Thr
595 600 605
Ile Lys Val Pro Ile Ser Arg Asn Ser Cys Gln Gly Tyr Lys Lys Leu
610 615 620
Gln Ala Val Leu Arg Ala Ile Met Leu Arg Arg Thr Lys Gly Thr Leu
625 630 635 640
Leu Asp Gly Lys Pro Ile Ile Asn Leu Pro Pro Lys Val Val Asn Leu
645 650 655
Ser Gln Val Asp Phe Ser Val Ala Glu Arg Ser Phe Tyr Lys Lys Leu
660 665 670
Glu Ala Asp Ser Arg Ser Gln Phe Lys Ala Tyr Ala Asp Ala Gly Thr
675 680 685
Leu Ser Gln Asn Tyr Ala Asn Ile Leu Leu Leu Leu Leu Arg Leu Arg
690 695 700
Gln Ala Cys Asp His Pro Gln Leu Val Lys Arg Tyr Asn Ser Asp Pro
705 710 715 720
Val Gly Lys Val Ser Glu Ala Ala Val Arg Arg Leu Pro Arg Glu Ala
725 730 735
Arg Ser Arg Leu Ile Asn Arg Leu Glu Ser Ser Ser Ala Ile Cys Tyr
740 745 750
Glu Cys Asn Glu Pro Pro Glu Lys Pro Val Val Thr Leu Cys Gly His
755 760 765
Ile Phe Cys Tyr Glu Cys Val Leu Glu Tyr Ile Thr Gly Asp Glu Asn
770 775 780
Thr Cys Pro Val Pro Arg Cys Lys Gln Gln Leu Ala Arg Asp Val Val
785 790 795 800
Phe Ser Glu Ser Ser Leu Arg Asn Cys Thr Ser Asp Asp Ser Gly Cys
805 810 815
Ser Ser Ser His Asp Asn Gly Leu Asp Arg Ser Val Phe Gln Lys Arg
820 825 830
Asp Phe Cys Ser Ser Lys Ile Lys Ala Val Leu Asp Ile Leu Gln Ser
835 840 845
Leu Ser Gln Pro Asp Ser Pro Asn Ser Ala Gln His Gly Gln Met Pro
850 855 860
Ser Ser Ser Arg Pro Tyr Asp Asp Asp Asp Val Thr Ile Val Glu Pro
865 870 875 880
Met Arg Leu His Ser Ser Ser Pro Ser Gln Gly Ala Val Lys Thr Ile
885 890 895
Ile Phe Ser Gln Trp Thr Gly Met Leu Asp Leu Val Glu Leu Arg Ile
900 905 910
Leu Glu Ser Gly Ile Glu Phe Arg Arg Leu Asp Gly Thr Met Ser Leu
915 920 925
Ala Ala Arg Asp Arg Ala Val Lys Glu Phe Ser Lys Lys Pro Asp Val
930 935 940
Lys Val Met Leu Met Ser Leu Lys Ala Gly Asn Leu Gly Leu Asn Met
945 950 955 960
Val Ala Ala Cys His Val Ile Leu Leu Asp Leu Trp Trp Asn Pro Thr
965 970 975
Thr Glu Asp Gln Ala Ile Asp Arg Ala His Arg Ile Gly Gln Thr Arg
980 985 990
Pro Val Thr Val Thr Arg Ile Thr Ile Lys Asp Thr Val Glu Asp Arg
995 1000 1005
Ile Leu Lys Leu Gln Glu Glu Lys Arg Thr Met Val Ala Ser Ala Phe
1010 1015 1020
Gly Glu Glu His Gly Gly Ser Ser Ala Thr Arg Leu Thr Val Asp Asp
1025 1030 1035 1040
Leu Lys Tyr Leu Phe Met Val
1045
<210> 126
<211> 993
<212> PRT
<213> 蓖麻
<400> 126
Met Glu Pro Ile Asp Ile Ser Ser Asp Ser Asp Val Val Ile Glu Asp
1 5 10 15
Asp Glu Asp Phe Glu Phe Glu Thr Ser Pro Val Arg Arg Pro Thr Asp
20 25 30
Ser Arg Ile Leu Pro Pro Trp Ala Ala Ile Ala Thr Thr Asp Ser Arg
35 40 45
Ser Ser Gly Tyr Gly Arg Gln Thr Gln Arg Asp Thr Ser Pro Lys Arg
50 55 60
Pro Tyr Ser Ser Asn Gly Ser Ser Ser Asn Trp His Ser Ser Asn Gly
65 70 75 80
Gly Ser Ser Asn Trp His Thr Ser Gln Ala Asp Asp Ser Leu Tyr Ala
85 90 95
Ile Gly Asn Gly Asn Ala Gly Leu Pro Arg Thr Val Asn Ser Arg Ile
100 105 110
Ala Asn Val Tyr Gly Thr Asp Tyr Glu Lys Leu Ser Ser Gln Gln Ala
115 120 125
Leu Lys Arg Thr Leu Pro Ser Ser Leu His Arg Ser Pro Ile Ser Ser
130 135 140
Ile Ser Asn Ser Leu Val Glu Gly Val Ser Ser Ser Gln Thr Arg Asp
145 150 155 160
Ile Tyr Gly Asn Ala Tyr His Pro Ala Gly Pro Ser Ser Ser His Ser
165 170 175
Lys Gly Phe Gly Arg Gly Asn Tyr Glu Glu Ala Ile Thr Tyr Val Ser
180 185 190
Asn Gly Ser Arg Thr Leu Pro Pro Ser Leu Met Arg Gly Lys Ser Thr
195 200 205
Pro Ser Ala Gln Phe Gly Leu Arg Asp Pro Ala Phe His Pro Met Ala
210 215 220
Gly Glu Glu Gly Val Ala Gly Ser Asp Glu Arg Leu Ile Tyr Gln Ala
225 230 235 240
Ala Leu Glu Asp Leu Asn Gln Pro Lys Val Glu Ala Thr Leu Pro Asp
245 250 255
Gly Leu Leu Ser Val Pro Leu Leu Arg His Gln Lys Ile Ala Leu Ala
260 265 270
Trp Met Leu Gln Lys Glu Thr Arg Ser Leu His Cys Leu Gly Gly Ile
275 280 285
Leu Ala Asp Asp Gln Gly Leu Gly Lys Thr Val Ser Met Ile Ala Leu
290 295 300
Ile Gln Met Gln Lys Phe Leu Gln Leu Lys Ser Lys Ser Glu Asp Gln
305 310 315 320
Ala Asn Lys Lys Ser Glu Ala Leu Asn Leu Asp Asp Asp Asp Glu Ser
325 330 335
Gly Arg Pro Gly Leu Asn Glu Val Lys Gln Val Gly Glu Tyr Asp Asp
340 345 350
Thr Thr Ser Val Pro Glu Ala Ser Asn Ser Thr Arg Val Phe Lys Arg
355 360 365
Lys Arg Leu Ala Ala Gly Thr Leu Val Val Cys Pro Ala Ser Ile Leu
370 375 380
Arg Gln Trp Ala Gly Glu Leu Asp Asp Lys Val Ala Asp Glu Ala Lys
385 390 395 400
Leu Thr Cys Leu Ile Tyr His Gly Gly Ser Arg Thr Lys Asp Pro Ala
405 410 415
Glu Leu Ala Lys Tyr Asp Val Val Leu Thr Thr Tyr Ser Ile Ile Thr
420 425 430
Asn Glu Val Pro Lys Gln Pro Leu Val Asn Glu Asp Glu Ala Asp Glu
435 440 445
Lys Asp Gly Glu Lys Cys Gly Leu Ser Ser Glu Phe Ser Ile Asn Lys
450 455 460
Lys Met Lys Lys Thr Thr Thr Val Ser Lys Lys Arg Lys Lys Gly Arg
465 470 475 480
Lys Gly Ile Asp Cys Ser Ser Asn Asp Tyr Asp Ser Gly Pro Leu Ala
485 490 495
Arg Val Gly Trp Ser Arg Val Ile Leu Asp Glu Ala Gln Thr Ile Lys
500 505 510
Asn His Arg Thr Gln Val Ala Arg Ala Cys Cys Ser Leu Arg Ala Lys
515 520 525
Thr Arg Trp Cys Leu Ser Gly Thr Pro Ile Gln Asn Ala Ile Asp Asp
530 535 540
Leu Tyr Ser Tyr Phe Arg Phe Leu Arg Tyr Asp Pro Tyr Ala Val Tyr
545 550 555 560
Lys Ser Phe Tyr Thr Thr Ile Lys Val Pro Ile Ser Arg Asn Ala Ile
565 570 575
Gln Gly Tyr Lys Lys Leu Gln Ala Val Leu Arg Ala Val Met Leu Arg
580 585 590
Arg Thr Lys Gly Thr Leu Ile Asp Gly Glu Pro Ile Val Lys Leu Pro
595 600 605
Pro Lys Ser Thr Cys Leu Thr Lys Val Asn Phe Ser Thr Glu Glu Arg
610 615 620
Ala Phe Tyr Thr Arg Leu Glu Ala Asp Ser Arg Ser Lys Phe Lys Ala
625 630 635 640
Tyr Ala Ala Ala Gly Thr Val Asn Gln Asn Tyr Ala Asn Ile Leu Leu
645 650 655
Met Leu Leu Arg Leu Arg Gln Ala Cys Asp His Pro Leu Leu Val Lys
660 665 670
Gly Leu Asn Ser Asp Ser Phe Gly Lys Asp Ser Ala Glu Met Ala Lys
675 680 685
Arg Leu Pro Asn Asp Met Val Ile Asn Leu Leu Ser Cys Leu Ala Thr
690 695 700
Ser Ser Ala Ile Cys Arg Ala Cys Asn Asp Pro Pro Glu Asp Pro Val
705 710 715 720
Val Thr Met Cys Asp His Val Phe Cys Tyr Gln Cys Val Ser Glu Tyr
725 730 735
Leu Thr Gly Asp Asp Asn Met Cys Pro Ala Arg Gly Cys Lys Glu Leu
740 745 750
Leu Gly Pro Asp Val Val Phe Ser Glu Ala Thr Leu Arg Ser Cys Met
755 760 765
Ser Asp Asn Leu Asp Ala Gly Pro Lys Arg Pro Glu Phe Asp Glu Arg
770 775 780
Ala Met Val Leu Gln Asn Glu Tyr Ser Ser Ser Lys Ile Arg Ala Val
785 790 795 800
Leu Glu Ile Leu Gln Ser His Cys Gln Val Lys Ser Pro Ser Pro Glu
805 810 815
Leu Gly Gly Ala Thr Glu Tyr Asn Gly Ser Ser Thr Ala Pro Ser Ser
820 825 830
Leu Val Ile Lys Ser Ile Ile Phe Ser Gln Trp Thr Ser Met Leu Asp
835 840 845
Leu Val Glu Phe Ser Leu Asn Gln His Cys Ile Gln Tyr Arg Arg Leu
850 855 860
Asp Gly Thr Met Thr Leu Gly Ala Arg Asp Arg Ala Val Lys Asp Phe
865 870 875 880
Asn Thr Asp Pro Glu Val Thr Val Met Leu Met Ser Leu Lys Ala Gly
885 890 895
Asn Leu Gly Leu Asn Met Val Ala Ala Cys His Val Ile Leu Leu Asp
900 905 910
Leu Trp Trp Asn Pro Thr Thr Glu Asp Gln Ala Val Asp Arg Ala His
915 920 925
Arg Ile Gly Gln Thr Arg Pro Val Thr Val Thr Arg Leu Thr Ile Lys
930 935 940
Asp Thr Val Glu Asp Arg Ile Leu Ala Leu Gln Glu Glu Lys Arg Arg
945 950 955 960
Met Val Ala Ser Ala Phe Gly Glu Asp Ala Ser Gly Gly Ser Ala Thr
965 970 975
Arg Leu Thr Val Glu Asp Leu Lys Tyr Leu Phe Met Gly Arg Ser Ala
980 985 990
Pro
<210> 127
<211> 1027
<212> PRT
<213> 大豆
<400> 127
Met Asp Ser Gln Asn Gly Cys Ile Tyr Ile Ser Ser Ser Asp Asp Asp
1 5 10 15
Leu Glu Glu Ile Glu Asp Pro Arg Arg Thr Leu Pro Gln Trp Ala Thr
20 25 30
Asn Thr Glu Lys Ser Ser Tyr Asn Gly Gly Trp Ser Arg Arg Asp Ser
35 40 45
Ser Ser Arg Gly Ala Asn Ser Ser Asn Pro Ser Ser Ser Asn Val Tyr
50 55 60
Asn His Ser Gln Val Lys Pro Gln Thr Leu Pro Val Ser Ser Thr Asn
65 70 75 80
Thr Leu Asn His Arg Ile Ala Arg Arg Asp Glu Pro Ser Tyr His Ala
85 90 95
Leu Asn Gly Asn Thr Ser Gln Gln Gln Thr Val Ser Ser Arg Ile Ser
100 105 110
Asn Ile His Gly Ala Asp Tyr Glu Lys Met Ser Ser Gln Gln Ala Phe
115 120 125
Lys Arg Thr Leu Pro Ser Ser Leu Gln Pro Ser Ala Thr Arg Ala Leu
130 135 140
Pro Ser Ser Phe Ala Ser Asp Ser Arg Leu Arg Asn Leu Lys Asp Asn
145 150 155 160
Ala Ser Ser Ser Gln Leu His Asp Ala Tyr Lys Asn Arg Pro His Gly
165 170 175
Val Gly Pro Ser Thr Ser Ser Asp Arg Gly Tyr Ile Arg Glu Asn Phe
180 185 190
Gly Arg Gly Tyr Asp Glu Asp Arg Phe Leu Tyr Gln Asn Gly Gly Asn
195 200 205
Arg Ile Leu Pro Ser Pro Leu Met Leu Gly Lys Val Ile Ser Pro Gln
210 215 220
Phe Ala Thr Ser Ser Glu Ser Ala Tyr Arg Ser Gly Ala Gly Asp Glu
225 230 235 240
Arg Ala Ala Glu Ser Asp Glu Arg Leu Ile Tyr Glu Ala Ala Leu Gln
245 250 255
Asp Ile Ser Gln Pro Lys Thr Glu Tyr Asp Leu Pro Ala Gly Val Leu
260 265 270
Ser Val Ser Leu Leu Arg His Gln Lys Ile Ala Leu Ala Trp Met Leu
275 280 285
Gln Lys Glu Thr Lys Ser Leu His Cys Leu Gly Gly Ile Leu Ala Asp
290 295 300
Asp Gln Gly Leu Gly Lys Thr Ile Ser Met Ile Ser Leu Ile Leu Ala
305 310 315 320
Gln Arg Thr Leu Gln Ser Lys Ser Lys Ile Asp Asp Thr Cys Ser His
325 330 335
Lys Thr Glu Ala Leu Asn Leu Asp Asp Asp Asp Asp Asn Gly Ser Val
340 345 350
Asp Val Glu Lys His Lys Asn Ser Glu Glu Ser Asp Asp Ile Lys Pro
355 360 365
Ser Arg Glu Pro Ser Ser Ser Thr Gln Ala Pro Gly Arg Lys Arg Pro
370 375 380
Ala Ala Gly Thr Leu Val Val Cys Pro Ala Ser Val Leu Arg Gln Trp
385 390 395 400
Ala Arg Glu Leu Asp Glu Lys Val Gly Asp Glu Lys Leu Ser Val Leu
405 410 415
Val Tyr His Gly Gly Ser Arg Thr Lys Asp Pro Val Glu Leu Ala Lys
420 425 430
Phe Asp Val Val Leu Thr Thr Tyr Ser Ile Val Thr Asn Glu Val Pro
435 440 445
Lys Gln Pro Leu Val Glu Glu Asp Asp Ile Asp Glu Lys Met Gly Glu
450 455 460
Arg Phe Gly Leu Ser Ser Glu Phe Ser Val Ser Lys Lys Arg Lys Lys
465 470 475 480
Pro Phe Asn Gly Asn Lys Lys Ser Lys Lys Gly Gly Lys Gly Ile Asp
485 490 495
Ser Ser Ser Ile Glu Cys Gly Ser Gly Pro Leu Ala Lys Val Gly Trp
500 505 510
Phe Arg Val Ile Leu Asp Glu Ala Gln Thr Ile Lys Asn His Arg Thr
515 520 525
Gln Val Ala Arg Ala Cys Cys Ser Leu Arg Ala Lys Arg Arg Trp Cys
530 535 540
Leu Ser Gly Thr Pro Ile Gln Asn Thr Ile Asp Asp Leu Tyr Ser Tyr
545 550 555 560
Phe Arg Phe Leu Lys Tyr Asp Pro Tyr Ala Val Tyr Lys Ser Phe Tyr
565 570 575
Asn Thr Ile Lys Val Pro Ile Ser Lys Asn Thr Ile Gln Gly Tyr Lys
580 585 590
Lys Leu Gln Ala Val Leu Arg Ala Ile Met Leu Arg Arg Thr Lys Gly
595 600 605
Thr Leu Leu Asp Gly Lys Pro Ile Ile Asn Leu Pro Pro Lys Thr Ile
610 615 620
Glu Leu Ser Lys Val Asp Phe Ser Ile Glu Glu Arg Ala Phe Tyr Thr
625 630 635 640
Lys Leu Glu Ser Asp Ser Arg Ser Gln Phe Lys Ala Tyr Ala Ala Ala
645 650 655
Gly Thr Val Ser Gln Asn Tyr Ala Asn Ile Leu Leu Met Leu Leu Arg
660 665 670
Leu Arg Gln Ala Cys Asp His Pro Leu Leu Val Lys Asp Phe Asp Ser
675 680 685
Asp Pro Val Gly Lys Asp Ser Val Glu Met Ala Lys Asn Leu Pro Arg
690 695 700
Glu Met Leu Ile Asn Leu Phe Asn Cys Leu Glu Ser Thr Phe Ala Ile
705 710 715 720
Cys Leu Val Cys Asn Asp Pro Pro Glu Glu Pro Val Ile Thr Met Cys
725 730 735
Gly His Val Phe Cys Tyr Gln Cys Val Ser Glu Tyr Leu Thr Gly Asp
740 745 750
Asp Asn Thr Cys Pro Ser Val Asn Cys Lys Glu Leu Ile Gly Asp Asp
755 760 765
Leu Val Phe Ser Lys Ala Thr Leu Arg Ser Cys Ile Ser Asp Asp Gly
770 775 780
Gly Ser Val Ser Phe Ala Asn Ser His Leu Cys Asp Tyr Ser Leu Val
785 790 795 800
Gln Gln Arg Asp Tyr Thr Ser Ser Lys Ile Lys Ala Val Leu Glu Val
805 810 815
Leu Gln Ser Asn Cys Lys Leu Lys Ile Ser Ser Ser Asp Leu Pro Asn
820 825 830
Ser Ser Gly Gly Cys Arg Asp Ser Pro Ser Leu Asp Asn Leu His Val
835 840 845
Glu Asp Cys Asp Ser Asp Val Arg Val Thr Lys His Thr Arg Arg Tyr
850 855 860
Ser Glu Ser Thr Thr Glu Gly Pro Ile Lys Ala Ile Val Phe Ser Gln
865 870 875 880
Trp Thr Ser Met Leu Asp Leu Val Glu Thr Ser Leu Lys Gln Phe Gly
885 890 895
Ile Gln Tyr Arg Arg Leu Asp Gly Arg Met Thr Leu Gly Ala Arg Asp
900 905 910
Lys Ala Val Lys Asp Phe Asn Thr Glu Pro Glu Ile Thr Val Met Leu
915 920 925
Met Ser Leu Lys Ala Gly Asn Leu Gly Leu Asn Met Val Ala Ala Cys
930 935 940
His Val Ile Leu Leu Asp Leu Trp Trp Asn Pro Thr Thr Glu Asp Gln
945 950 955 960
Ala Ile Asp Arg Ala His Arg Ile Gly Gln Thr Arg Pro Val Thr Val
965 970 975
Thr Arg Ile Thr Ile Lys Asp Thr Val Glu Asp Arg Ile Leu Ala Leu
980 985 990
Gln Asp Asp Lys Arg Lys Met Val Ala Ser Ala Phe Gly Glu Asp His
995 1000 1005
Ala Gly Ala Ser Gly Thr Arg Leu Thr Val Asp Asp Leu Lys Tyr Leu
1010 1015 1020
Phe Met Val
1025
<210> 128
<211> 1033
<212> PRT
<213> 玉蜀黍
<400> 128
Met Asn Glu Asn Ser Ala Ile Ile Ile Ser Asp Ser Asp Asp Asp Ser
1 5 10 15
Ile Gly Ser Ile Phe Asp Asp Asp Asp Gln Pro Ala Ser Ala Ile Ala
20 25 30
Arg Leu Asp Gln Asn Ala Glu Gly Arg Ile Ile Asn Phe Glu Asp Glu
35 40 45
Asp Trp Gln Arg Ser Ala Pro Ala Pro Pro Ser Thr Lys Pro Thr Glu
50 55 60
Asn Asn Asn Gly Gln Tyr Arg Ile Leu Pro Ala Ser Leu Thr Asn Gly
65 70 75 80
Arg His Ala Glu Ser Ser Arg Tyr Thr Phe Gly Ser Val Asp Arg Ile
85 90 95
His Pro His Ala Asn Pro Tyr Met Ala Met Arg Pro Gly His Ser Thr
100 105 110
Ser Ser Arg Ile Asp Ser Val Val Glu Lys His Asn Ser Ser Thr Ala
115 120 125
Asp Ala Asn Asp Asn Asn Lys Arg Val Leu Pro Ser Ser Phe Ser Asn
130 135 140
Gly Asn Thr Ser Lys Ser Met His Pro Ile Val Ala Ser Glu Thr Arg
145 150 155 160
Lys Leu Pro Leu Pro Leu Ser Thr Lys Lys Ser Pro Asn Ile Gly Glu
165 170 175
Asn Arg Met Gly Thr Asn Ile Ala Asn Gly Asn Leu Gln Pro Ser Ser
180 185 190
Ser Ile Met Ala Arg Gly Thr Ser Ser Thr Leu Asn Thr His Lys Val
195 200 205
Asp Asp Asp Asp Asp Val Ile Val Tyr Gly Gly Ser Ser Ser His Arg
210 215 220
Val Leu Pro Ser Thr Phe Glu Ala Thr Asn Ser Asn Asn Ser Glu Val
225 230 235 240
Ala Lys Gly Phe Glu Thr His Ser Arg Leu Asn Pro Glu Asn Arg Val
245 250 255
Leu Asp Tyr Ala Glu Arg Ala Val Tyr Gln Glu Ala Leu Gln Asn Ile
260 265 270
Ser Arg Glu Lys Ser Glu Asp Asp Leu Pro Glu Gly Val Leu Ala Val
275 280 285
Pro Leu Leu Arg His Gln Lys Met Ala Leu Ala Trp Met Val Ser Lys
290 295 300
Glu Asn Ser Ser His Cys Ala Gly Gly Ile Leu Ala Asp Asp Gln Gly
305 310 315 320
Leu Gly Lys Thr Val Ser Thr Ile Ala Leu Ile Gln Lys Gln Arg Met
325 330 335
Glu Gln Ser Lys Phe Met Phe Val Asp Ser Asp Arg Leu Lys Ser Glu
340 345 350
Ala Leu Asn Leu Asp Glu Asp Asp Glu Gly Glu Gln Thr Val Ser Asn
355 360 365
Glu Pro Lys Lys Asp Gln Gly Ala Cys Ser Leu Ser Thr Ser Ala Gly
370 375 380
Thr Ser Ala Glu Leu Phe Val Asn Gln Pro Asn Asn Val Val Asn Lys
385 390 395 400
Met Val Glu Thr Lys Ala Glu Arg Lys Lys Lys Ala Lys Val Ser Thr
405 410 415
Ser Ser Ala Ser Thr Ser Arg Ser Met Thr Arg Pro Ala Ala Gly Thr
420 425 430
Leu Val Val Cys Pro Ala Ser Val Leu Lys Gln Trp Ser Asn Glu Leu
435 440 445
Thr Asp Lys Val Ser Glu Ser Ala Lys Leu Ser Val Leu Val Tyr His
450 455 460
Gly Gly Ala Arg Thr Lys Asp Pro Arg Glu Leu Ala Lys Tyr Asp Val
465 470 475 480
Val Val Thr Thr Tyr Thr Ile Val Ala Asn Glu Val Pro Lys Gln Met
485 490 495
Ala Asp Asp Asp Ala Asp Gln Lys Asn Ser Glu Glu Pro Ser Ala Ser
500 505 510
Asn Lys Arg Lys Pro Ser Ala Asn Met Gln Asn Lys Ala Lys Lys Lys
515 520 525
Lys Lys Lys Leu Lys Asp Ser Asn Phe Asp Leu Asp Ser Gly Pro Ile
530 535 540
Ala Arg Val Arg Trp Phe Arg Val Val Leu Asp Glu Ala Gln Thr Ile
545 550 555 560
Lys Asn Phe Arg Thr Val Val Ala Arg Ala Cys Cys Gly Leu Arg Ala
565 570 575
Lys Arg Arg Trp Cys Leu Ser Gly Thr Pro Ile Gln Asn Ala Ile Asp
580 585 590
Asp Leu Phe Ser Tyr Phe Arg Phe Leu Lys Tyr Asp Pro Tyr Cys Thr
595 600 605
Tyr Asn Ser Phe Cys Thr Met Ile Lys His Pro Ile Ala Arg Asp Ala
610 615 620
Ile Asn Gly Tyr Lys Lys Leu Gln Ala Val Leu Lys Val Val Leu Leu
625 630 635 640
Arg Arg Thr Lys Glu Thr Val Ile Asn Gly Lys Pro Ile Ile Asn Leu
645 650 655
Pro Pro Lys Thr Ile Asn Leu Asn Lys Val Asp Phe Thr Gln Glu Glu
660 665 670
Arg Ser Phe Tyr Leu Met Leu Glu Glu Arg Ser Arg Gln Gln Phe Lys
675 680 685
Ala Phe Ala Ala Ala Gly Thr Leu Lys Gln Asn Tyr Ala Asn Ile Leu
690 695 700
Leu Met Leu Leu Arg Leu Arg Gln Ala Cys Asp His Pro Ile Leu Val
705 710 715 720
Lys Gly Asn Gln Ser Glu Tyr Gly Gly Asp Gly Ser Ile Glu Met Ala
725 730 735
Lys Lys Leu Pro Lys Glu Val Val Ile Asp Leu Leu Ala Lys Leu Glu
740 745 750
Val Gly Ser Thr Leu Cys Gly Leu Cys Asn Asp Thr Pro Glu Asp Ala
755 760 765
Ile Val Thr Ile Cys Gly His Val Phe Cys Tyr Gln Cys Ile His Glu
770 775 780
Arg Ile Thr Thr Asp Glu Asn Met Cys Pro Ala Pro Asn Cys Ser Arg
785 790 795 800
Thr Leu Gly Leu Glu Leu Leu Phe Ser Ser Gly Ala Leu Lys Ile Cys
805 810 815
Ile Ser Gly Lys Ser Ser Ser Ala Val Ala Ser Ser Ser Ser Asp Asn
820 825 830
Glu Ser Ser Ser Ile Ser Gln Ser Ser Phe Val Ser Ser Lys Ile Gln
835 840 845
Ala Ala Ile Asp Ile Leu Asn Ser Ile Ile Val Met Asp Pro Leu Thr
850 855 860
Glu Ser Tyr Thr Met Glu Ser Ser Arg Ser Gly Leu Gly Pro Val Lys
865 870 875 880
Ala Ile Val Phe Ser Gln Trp Thr Gly Met Leu Asp Leu Leu Glu Leu
885 890 895
Ser Leu Asn Ile Asn Cys Ile Gln Tyr Arg Arg Leu Asp Gly Thr Met
900 905 910
Ser Leu Asn Leu Arg Glu Lys Asn Val Lys Asp Phe Asn Thr Asp Pro
915 920 925
Glu Val Arg Val Met Ile Met Ser Leu Lys Ala Gly Asn Leu Gly Leu
930 935 940
Asn Met Val Ser Ala Cys His Val Ile Leu Leu Asp Leu Trp Trp Asn
945 950 955 960
Pro Tyr Ala Glu Asp Gln Ala Val Asp Arg Ala His Arg Ile Gly Gln
965 970 975
Thr Arg Pro Val Thr Val Ser Arg Leu Thr Val Lys Asp Thr Val Glu
980 985 990
Asp Arg Ile Leu Ala Leu Gln Glu Glu Lys Arg Thr Met Val Asn Ser
995 1000 1005
Ala Phe Gly Asp Asp Lys Ala Gly Gly His Ala Thr Arg Leu Thr Val
1010 1015 1020
Glu Asp Leu Arg Tyr Leu Phe Arg Ile
1025 1030
<210> 129
<211> 1314
<212> PRT
<213> 蒺藜苜蓿
<400> 129
Met Ala Asp Asp Gly Phe Gln Phe Ser Thr Leu Phe Ala Asp Asp Asp
1 5 10 15
Ser Asp Asp Glu Lys Leu Ala Met Asp Ile Glu Ser Phe Leu Ser Val
20 25 30
Leu Asp Glu Asp Cys Val Pro Ser Glu Ser Ser Pro Glu Asp Ser Ser
35 40 45
Leu Lys Asp Val Ser Pro Gly Glu Ser Gly Ile His Asp Asp Phe Leu
50 55 60
Leu Gln Asn Gly Asn Ser Leu Leu Asp Ser Glu His Glu Asn Gln Gly
65 70 75 80
Pro Ser Ser Arg Thr Cys Ser Ser Pro Asn Val Phe Ala Gly Gly Tyr
85 90 95
Arg Asp Ser Phe Ser Val Ala Glu Ser Asp Glu Thr Cys Tyr Ala Glu
100 105 110
Arg Ala Gly Val Ser Glu His Glu Met Pro Ser Tyr Ser Val Gly Thr
115 120 125
Ser Ser Asn Pro Ser Leu Trp Lys Asp Asp Asp Tyr Ser Gln Ile Lys
130 135 140
His Val Val Asp Asp Val Glu Ser Glu Tyr Ala Ser His Ser Ser Ile
145 150 155 160
Ile Glu Asn Val Asp Gly Thr Phe Glu Asp Tyr Gly Thr Ala Leu Lys
165 170 175
Asp Thr Ile Gly Val Ser Arg Gln Pro Glu Asn Asp Ser Cys Thr Ser
180 185 190
Phe Glu Met Pro Phe Val Asp Val Asp Ile Pro Thr His Phe Gly Asn
195 200 205
Ser Ala Asp Ser Thr Phe Cys Gln Gly Ser Asn Val Pro Ser Asp Phe
210 215 220
Ser Gly Tyr Tyr Ser Ser Leu Asn Cys Tyr Gln Gly Ile Asp Val Arg
225 230 235 240
Pro Val Val Thr Gly Ser Ser Gly Tyr Phe Pro Asn Gly Val Gly Ser
245 250 255
Glu Phe Trp Lys Asn Glu Glu Pro Val Arg Asn Met Lys Val Glu Lys
260 265 270
Thr Glu Phe Leu Thr Asp Thr Thr Asn Val Ile Gly Gly Met Asp Leu
275 280 285
Ser Thr Ile Gly Arg Ile Pro Phe His Asp Ser Gln Phe Met Pro Val
290 295 300
Asn Asn Glu Tyr Pro Ser Phe Phe Pro Gly Asn Ala Lys Phe Glu Asp
305 310 315 320
Gly Glu Ser Val Gln Gln Ser Ser Cys Val Pro Tyr Ile Ser Ser Glu
325 330 335
Gly Gln Ser Phe Asn Val Lys Ala Glu Gly Asp Glu Met Val Met Pro
340 345 350
Tyr Gln Asn Thr Phe His Asn Asp Asn Ala Gly Leu Glu Val Lys Gln
355 360 365
Leu Pro Gly Ile Phe Pro Thr Thr Gly Tyr Gln Asn Tyr Asp Phe Phe
370 375 380
Lys Val Glu Asp Ser Asn Ala Ile Val Thr Thr Glu Asp Ala Asn Tyr
385 390 395 400
Tyr Gln Asp Leu Ile Gly Glu Thr Ala Asn Lys Phe Pro Gly Asn Met
405 410 415
Gly Asn Leu Asn Phe Arg Ser Leu Asp Lys Ser Leu Ser Ile Ala Arg
420 425 430
Ala Ser Ile Ala Asn Gly Asn Gln Tyr Asn Cys Ser Met Ser Glu Leu
435 440 445
Glu Ser Lys Pro Ser Glu Cys Lys Ser Ile Asp Ser Gln Leu Ser Lys
450 455 460
Arg Ser Thr Glu Gly Ser Asn Asp Glu Asp Asp Cys Asp Val Cys Ile
465 470 475 480
Ile Glu Asp Ile Ser His Pro Ala Pro Thr Ser Arg Ser Ala Glu Phe
485 490 495
Asn Ser Leu Asn Met Ser Gln Ser Ser Arg Phe Asp Tyr Thr Gln Pro
500 505 510
Tyr Met Ala Gly Gly Thr Arg Pro Lys Ala His Asp Glu Gln Tyr Ile
515 520 525
Leu Arg Ala Ala Leu Gln Asp Ile Ser Gln Pro Lys Ser Glu Val Thr
530 535 540
Pro Pro Asp Gly Leu Leu Ala Val Pro Leu Leu Arg His Gln Glu Cys
545 550 555 560
Gly Ser Asp Gly Leu Asp Leu Glu Phe Lys Ile Ala Leu Ser Trp Met
565 570 575
Val Gln Lys Glu Thr Ser Ser Leu Tyr Cys Ser Gly Gly Ile Leu Ala
580 585 590
Asp Asp Gln Gly Leu Gly Lys Thr Val Ser Thr Ile Ala Leu Ile Leu
595 600 605
Lys Glu Arg Pro Pro Leu Leu Lys Thr Cys Asn Asn Ala Gln Lys Ser
610 615 620
Val Leu Gln Thr Met Asp Leu Asp Asp Asp Pro Leu Pro Glu Asn Gly
625 630 635 640
Leu Val Lys Lys Glu Ser Thr Val Cys Gln Asp Ala Ser Asp Arg Asn
645 650 655
Ala Thr Thr Ser Ala Asn Leu Ser Val His Ala Lys Gly Arg Pro Ser
660 665 670
Ala Gly Thr Leu Val Val Cys Pro Thr Ser Val Leu Arg Gln Trp Ala
675 680 685
Asp Glu Leu His Asn Lys Val Thr Cys Lys Ala Asn Leu Ser Val Leu
690 695 700
Val Tyr His Gly Ser Ser Arg Thr Lys Asp Pro Tyr Glu Leu Ala Lys
705 710 715 720
Tyr Asp Val Val Leu Thr Thr Tyr Ser Ile Val Ser Met Glu Val Pro
725 730 735
Lys Gln Pro Leu Val Asp Lys Asp Asp Lys Asp Asp Lys Glu Lys Gly
740 745 750
Ile Tyr Glu Asp His Pro Val Pro Asn Arg Lys Arg Lys Cys Pro Pro
755 760 765
Ser Ser Lys Ser Gly Lys Lys Ala Leu Asn Ser Met Met Leu Glu Ala
770 775 780
Ala Ala Arg Pro Leu Ala Lys Val Ala Trp Phe Arg Val Val Leu Asp
785 790 795 800
Glu Ala Gln Ser Ile Lys Asn His Arg Thr Gln Val Ala Arg Ala Cys
805 810 815
Trp Gly Leu Arg Ala Lys Arg Arg Trp Cys Leu Ser Gly Thr Pro Ile
820 825 830
Gln Asn Ala Ile Asp Asp Leu Tyr Ser Tyr Phe Arg Phe Leu Arg Tyr
835 840 845
Asp Pro Tyr Ala Val Tyr Thr Ser Phe Cys Ser Thr Ile Lys Ile Pro
850 855 860
Ile Asn Arg Asn Pro Ser Lys Gly Tyr Arg Lys Leu Gln Ala Val Leu
865 870 875 880
Lys Thr Ile Met Leu Arg Arg Thr Lys Gly Thr Leu Leu Asp Gly Glu
885 890 895
Pro Ile Ile Ser Leu Pro Pro Lys Ser Val Glu Leu Arg Lys Val Glu
900 905 910
Phe Ser Gln Glu Glu Arg Asp Phe Tyr Ser Lys Leu Glu Ala Asp Ser
915 920 925
Arg Ala Gln Phe Gln Glu Tyr Ala Asp Ala Gly Thr Val Lys Gln Asn
930 935 940
Tyr Val Asn Ile Leu Leu Met Leu Leu Arg Leu Arg Gln Ala Cys Asp
945 950 955 960
His Pro Leu Leu Val Lys Arg Tyr Asn Ser Thr Thr Leu Trp Lys Ser
965 970 975
Ser Val Glu Thr Ala Met Lys Leu Pro Arg Glu Lys Gln Leu Phe Leu
980 985 990
Leu Lys Cys Leu Glu Ala Ser Leu Ala Leu Cys Gly Ile Cys Asn Asp
995 1000 1005
Ala Pro Glu Glu Ala Val Val Ser Val Cys Gly His Val Phe Cys Asn
1010 1015 1020
Gln Cys Ile Cys Glu His Leu Thr Gly Glu Asp Asn Gln Cys Pro Ala
1025 1030 1035 1040
Thr Asn Cys Lys Thr Arg Leu Asn Met Ser Ala Val Phe Pro Lys Ala
1045 1050 1055
Thr Leu Asn Ser Ser Ile Ser Asp Pro Ala Cys Asp His Leu Pro Gly
1060 1065 1070
Ser Glu Val Glu Asp Ser Glu Pro Cys Ser Arg Thr Gln Pro Cys Asp
1075 1080 1085
Ser Ser Lys Ile Arg Ala Ala Leu Glu Val Leu Gln Ser Leu Ser Lys
1090 1095 1100
Pro Gln Cys His Thr Ser Gln Arg Ser His Val Gln Ser Thr Ser Arg
1105 1110 1115 1120
Glu Ser Ser Asp Cys Ser Ser Thr Ser Ala Asn Asn Gly Lys Ser Ile
1125 1130 1135
Ser Asp Val Pro Glu Lys Lys Ala Met Phe Met Glu Lys Ser Ser Asn
1140 1145 1150
Asp Ser Val Gly Ser Leu Gly Glu Lys Ala Ile Val Phe Ser Gln Trp
1155 1160 1165
Thr Gly Met Leu Asp Leu Leu Glu Ala Cys Leu Lys Asp Ser Ser Ile
1170 1175 1180
Gln Tyr Arg Arg Leu Asp Gly Thr Met Ser Val Leu Ala Arg Asp Lys
1185 1190 1195 1200
Ala Val Lys Asp Phe Asn Thr Leu Pro Glu Val Ser Val Met Ile Met
1205 1210 1215
Ser Leu Lys Ala Ala Ser Leu Gly Leu Asn Met Val Ala Ala Cys His
1220 1225 1230
Val Leu Met Leu Asp Leu Trp Trp Asn Pro Thr Thr Glu Asp Gln Ala
1235 1240 1245
Ile Asp Arg Ala His Arg Ile Gly Gln Thr Arg Pro Val Thr Val Leu
1250 1255 1260
Arg Leu Thr Val Lys Asp Thr Val Glu Asp Arg Ile Leu Ala Leu Gln
1265 1270 1275 1280
Gln Lys Lys Arg Thr Met Val Ala Ser Ala Phe Gly Glu Asp Gly Thr
1285 1290 1295
Ser Gly Arg Gln Thr Arg Leu Thr Val Asp Asp Leu Lys Tyr Leu Phe
1300 1305 1310
Met Met
<210> 130
<211> 1385
<212> PRT
<213> 小立碗藓
<400> 130
Met Glu Tyr Arg Leu Val Ser Ser Pro Arg Glu Pro Val Leu Thr Ser
1 5 10 15
Ser Ser Ile Asp Ile Thr Ser Asn Val His Pro Ile Thr Phe Gly Ser
20 25 30
Asp Gly Gln Val Ser Val Leu Pro Thr Thr Asp Ser Val Gln Gln Thr
35 40 45
Asn Asp Asn Glu Ile Val Gln Arg Ala Leu Val Lys Phe Met Gln Asp
50 55 60
Val Arg Ser Phe Val Arg Gln Gly Asn Trp Lys Glu Ser Ser Thr Gly
65 70 75 80
Leu Phe His Arg Ser Ser Thr Asp Cys Ala Arg Asp Pro Phe Gln Val
85 90 95
Ser Val Ala Glu Thr Ile Gly Ser Leu Glu Trp Gly Ile Phe Gly Ser
100 105 110
Ala Gly Met Gly Glu Ser Arg Leu Arg Gly Asp Tyr Ser Cys Ser Glu
115 120 125
Leu Gly Gln Asp Gly Met Gly Glu Leu Glu Gly Ala Gln Asn Asn Trp
130 135 140
Arg Glu Gly Glu Ala Asp Val Glu Asp Gly Ser Glu Pro Val Arg Asp
145 150 155 160
Glu Thr Asp Gln Arg Asp Val Tyr Leu Asp Glu Leu Leu Ser Val Ile
165 170 175
Glu Asp Asp Pro Asp Ser Arg Val Leu Arg Ile Pro Pro Gly Leu Glu
180 185 190
Ser Arg Ser Gly Glu Ser Cys Arg Ser Cys Ser Tyr Asp Leu Glu Phe
195 200 205
Glu Val Tyr Ala Pro Leu Thr Ile Leu Val Ser Pro Gln Ala Ala Pro
210 215 220
Arg Phe Gln Ala Thr Ser Ala Met Asp Glu Val Leu Glu Thr Asp Gly
225 230 235 240
Thr Met Pro Leu Leu Gly Pro Ala Val Ser His Gln Glu Leu Gly Ile
245 250 255
Ala Pro Tyr Ala Pro Ala Val Pro Glu Gly His Tyr Gly Leu Ser Leu
260 265 270
Ala Pro Ser Phe Asn His Thr Thr Ser Glu Val Phe Trp Gln Arg Asp
275 280 285
Leu Asn Gly Ser Gly Glu Gly Phe Glu Glu Thr Ser Asp Glu Val Leu
290 295 300
Ser Leu Glu Asp Thr Glu Gln Asp Thr Ile Gln Tyr Phe Pro Glu Leu
305 310 315 320
Thr Gly Asp Leu Asp Tyr Asp Arg Pro Gln Gln Asn Asp Gly Ser Val
325 330 335
Leu Ala Ser Asn Cys Tyr Ser Glu Arg Leu Ser Pro Gly Val Ser Arg
340 345 350
Pro Ser Arg Ser Thr Ser Ser Ser Pro Ile Ala Met Phe Asp Asn Thr
355 360 365
Ser Arg His Gly Leu Val Phe Asp Ser Pro Phe Glu Arg Asn Ser Ile
370 375 380
Val Glu Asn Pro Arg Gln Phe Thr Ile Leu Asn Gly His Ala Ile Val
385 390 395 400
Asp Leu Thr Ser Asp Asp Ser Asp Gly Asp Glu Glu Glu Ser Glu Leu
405 410 415
Lys Glu Ile Asp Gly Arg Ser Arg Gly Lys Arg Lys Leu Pro His Asp
420 425 430
Glu Thr Glu Gly Asp Arg Lys Arg Leu Arg Glu Thr Ala Asn Ser Ser
435 440 445
Tyr Met Gln Asn Ile Gly Thr Gly Phe Pro Tyr Thr Gln Ile Ala Lys
450 455 460
Lys Pro Cys Glu Thr Ile Ser Phe Pro Asn Arg Ser Gln Ser Trp Ser
465 470 475 480
Pro Ser Ser Leu Lys Tyr Ser Ser Gln Leu Gly Asn Leu Glu Ser Ser
485 490 495
Val Thr Thr Ala Thr Ser Leu Lys Asn Ile Pro Gly Tyr Glu Ser His
500 505 510
Lys Thr Thr Ser Ala Arg Arg Asp Thr Tyr Thr Ser Ser Leu Thr Asn
515 520 525
Met Ala Gln Arg Ser Cys Ser Gly Glu Val Ser Thr Ser Tyr Asp Arg
530 535 540
Gly Ile Ser Arg Val Leu Pro Gly Thr Val Ala Arg Ser Met Ala Gly
545 550 555 560
Leu Val Asn Gly Met Arg Val Ala Ala Thr Ile Asp His Arg Leu Ala
565 570 575
Leu Ala Met Asp Pro Val Lys Arg Ser Glu Glu Leu Ala Ile Gln Ala
580 585 590
Val Val Gln Ala Phe Ser Leu Gly Asp Glu Lys Glu Glu Leu Thr Pro
595 600 605
Asp Glu Asp Leu Leu Thr Met Thr Leu Leu Lys His Gln Arg Ile Ala
610 615 620
Leu Ala Trp Met Val Asn Arg Glu Ser Gly Arg His Glu Pro Cys Gly
625 630 635 640
Gly Ile Leu Ala Asp Asp Gln Gly Leu Gly Lys Thr Ile Ser Thr Ile
645 650 655
Ser Leu Ile Leu Lys Asn Arg Ala Pro Ile Leu Lys Ser Gly Ser Thr
660 665 670
Ser Ala Gln Ser Val Gln Leu Glu Gly Ser Thr Val Asp Leu Asp Ala
675 680 685
Tyr Glu Asp Asp Glu Asp Gln Leu Leu Leu Lys Lys Glu Phe Glu Asn
690 695 700
Gly Gln Trp Pro Ala Ser Ala Leu Ile Glu Asn Gly Asn Gln Leu Gln
705 710 715 720
Gln Asp Glu Pro Lys Ser Ser Gln Pro Ser Ser Lys Gly Arg Pro Ala
725 730 735
Ala Gly Thr Leu Val Val Cys Pro Thr Ser Val Leu Arg Gln Trp Ala
740 745 750
Gln Glu Ile Arg Asp Lys Val Ser Ile Lys Ala Asp Val Ser Val Leu
755 760 765
Val Tyr His Gly Ser Asn Arg Ile Lys Asp Pro His Glu Ile Ala Lys
770 775 780
Phe Asp Val Val Leu Ser Thr Tyr Ser Ile Val Ser Met Glu Val Pro
785 790 795 800
Lys Gln Ala Leu Pro Glu Glu Lys Glu Val Asp Asn Arg Arg Ser Ala
805 810 815
Phe Asp Tyr Gly Ile Ser Gln Phe Thr Lys Pro Lys Lys Asp Lys Pro
820 825 830
Glu Lys Val Lys Lys Ala Lys Ala Lys Gly Lys Gly Ala Gly Ala Asp
835 840 845
Gly Asp Ser Ser Asp Ser Gly Pro Leu Ala Arg Val Ala Trp Phe Arg
850 855 860
Val Val Leu Asp Glu Ala Gln Ser Ile Lys Asn Tyr Arg Thr Gln Val
865 870 875 880
Ala Arg Ala Val Trp Gly Leu Arg Ala Lys Arg Arg Trp Cys Leu Ser
885 890 895
Gly Thr Pro Ile Gln Asn Ser Val Asp Asp Leu Phe Ser Tyr Phe Arg
900 905 910
Phe Leu Arg Tyr Ser Pro Trp Gly Asp Val Tyr Lys Lys Phe Gln Arg
915 920 925
Asp Ile Lys Asp Pro Val Gly Arg Asn Pro Thr Glu Gly Tyr Lys Lys
930 935 940
Leu Gln Ala Ile Leu Lys Pro Ile Val Leu Arg Arg Thr Lys Thr Ser
945 950 955 960
Phe Leu Asp Gly Lys Pro Ile Val Asn Leu Pro Gln Arg Ile Val Lys
965 970 975
Leu Gln Gln Thr Glu Phe Ser Leu Asn Glu Arg Ser Phe Tyr Ser Asn
980 985 990
Leu Glu Thr Glu Ser Arg Ala Gln Phe Gln Met Tyr Ala Ala Ala Gly
995 1000 1005
Thr Val Gln Asn Asn Tyr Val Asn Ile Leu Trp Met Leu Leu Arg Leu
1010 1015 1020
Arg Gln Ala Cys Asp His Pro Met Leu Val Lys Lys Cys Ala Lys Ser
1025 1030 1035 1040
Glu Ala Leu Gln Lys Thr Thr Leu Glu Ala Val Arg Lys Leu Pro Pro
1045 1050 1055
His Gln Arg Ala Ala Leu Ile Gln Cys Leu Glu Gly Gly Arg Ala Ile
1060 1065 1070
Cys Tyr Ile Cys Gln Asp Ala Pro Glu Asp Pro Val Val Ser Ile Cys
1075 1080 1085
Ala His Val Phe Cys Arg Gln Cys Val Ser Glu Gln Met Asn Gly Asp
1090 1095 1100
Asp Thr Thr Cys Arg Phe Pro Lys Cys Lys Lys Ser Leu Asn Val Ser
1105 1110 1115 1120
Leu Leu Tyr Thr Leu Ser Ala Leu Lys Asp Ser Gly Val Cys Glu Glu
1125 1130 1135
Ser Ser Ser Leu Ile Lys Glu Glu Lys Ser Ser Glu Pro Ala Ile Thr
1140 1145 1150
Glu Leu Asp Gln Ser Trp Lys Thr Ser Ser Lys Ile Asp Ala Met Met
1155 1160 1165
Asn Thr Leu Gln Ala Leu Pro Lys Val Ile Val Leu Val Glu Asp Gly
1170 1175 1180
Lys Ile Val Lys Gly Pro Lys Ala Glu Thr Leu Leu Lys Ala Glu Ala
1185 1190 1195 1200
Val Glu Ile Asp Gln Gly Glu Thr Leu Ser Ser Gly Leu Pro Val Val
1205 1210 1215
Ser Glu Thr Thr Val Ser Lys Ile Asp Lys Val Glu Ser Thr Glu Lys
1220 1225 1230
Ala Ile Val Phe Ser Gln Trp Thr Ser Met Leu Asp Leu Leu Glu Thr
1235 1240 1245
Pro Leu Lys Lys Ser Gly Leu Cys Tyr Arg Arg Leu Asp Gly Thr Met
1250 1255 1260
Ser Val Val Ala Arg Asp Arg Ala Val Ser Asp Phe Asn Thr Leu Pro
1265 1270 1275 1280
Glu Val Thr Val Met Ile Met Ser Leu Lys Ala Ala Ser Leu Gly Leu
1285 1290 1295
Asn Met Val Ala Ala Asn His Val Leu Leu Leu Asp Val Trp Trp Asn
1300 1305 1310
Pro Thr Thr Glu Asp Gln Ala Ile Asp Arg Ala His Arg Ile Gly Gln
1315 1320 1325
Thr Arg Thr Val Asn Val Ser Arg Phe Thr Ile Lys Asn Thr Ile Glu
1330 1335 1340
Asp Arg Ile Leu Ala Leu Gln Glu Arg Lys Arg Gln Ile Val Ala Ser
1345 1350 1355 1360
Ala Phe Gly Glu Asn Ser Gly Gly Glu Gln Lys Asn Arg Leu Thr Val
1365 1370 1375
Glu Asp Leu Arg Tyr Leu Phe Arg Val
1380 1385
<210> 131
<211> 1255
<212> PRT
<213> 两色蜀黍
<400> 131
Met Ala Glu Glu Pro Ala Val Gly Tyr Asp Gly Phe Glu Ala Ala Gly
1 5 10 15
Asp Ala Gly Ala Gly Gly Ala Glu Asp Asn Leu Ser Met Pro Leu Gly
20 25 30
Asp Phe Met Ala Phe Leu Glu Thr Glu Pro Ala Pro Pro Glu Glu Gly
35 40 45
Gly Glu Glu Glu Glu Glu Gln Glu Gln Leu Gln Pro Gly Val Asn Gln
50 55 60
Gly Cys Phe Glu Met Pro Ala Asn Thr Asp Gly Ser Glu Asp Leu Leu
65 70 75 80
Gln Ser His Glu Glu Met Phe Glu Asn Ala Asp Leu Trp Ser Asn Tyr
85 90 95
Ser His Val Asp Thr Ser Glu Cys His Met Glu Leu Ser Gly Gly Glu
100 105 110
Gln Met Ile Asp His Thr Glu Ala Ser Pro Tyr Glu Phe Phe Ser Asn
115 120 125
Asp Leu Gln Asn Gln Ser Arg Thr Ser Asn Leu Asp Asn Glu His Phe
130 135 140
Pro Arg Asp Ala Ser Asn His Ala Asn Val Glu Glu Val Ala Gly Pro
145 150 155 160
Pro Tyr Glu Asp Leu Ser Asn Gly Leu Tyr Leu Arg Gln Gln Thr Leu
165 170 175
Tyr Ser Gly Gln Thr Gln Phe Gln Val Glu Asn Asn Thr Glu Gly Met
180 185 190
Glu Thr Gln Met Asn Thr Tyr Phe Ser Gly Gly Ile Ser Thr Glu His
195 200 205
Ser Asp Leu Met Leu Gly Ser Thr Gly Gln Asp Gly Asp His Phe Thr
210 215 220
Ser Met Gly Met Phe Ser Leu Thr His Lys Thr Asp Val Pro Asp Ile
225 230 235 240
Ser Cys Thr Glu Phe Asn Met Gly Glu Gly Thr Glu Ser Ile Arg Asn
245 250 255
Gly Asn Ser Ser Cys Leu Thr Met Gln Glu Ala Glu Cys Gly Gly Tyr
260 265 270
Pro His Pro Asp Cys Ile Ser Val Asp Met Val Asp Glu Arg Ser Leu
275 280 285
His Asp Leu Pro His Gly Phe Ser Gln Asn Asn Glu Gln Tyr Glu Met
290 295 300
Glu Gln Phe Pro Gln Ser Ile Cys Glu Ser Gly Ser Met Gln Met Gly
305 310 315 320
Ser Pro Asp Gln Tyr Cys Asp Asp Thr Ser Leu Ser Asp Phe Tyr Met
325 330 335
Asp Val Ser Ser Pro Glu Ser Ile Ser Cys Glu Gln Asn Gln Ser Glu
340 345 350
Asp Ile Cys Phe Lys Ser Glu Ser Ser Thr Asp Ser Ser Pro Ile Pro
355 360 365
Ser Ser Arg Asn Ser Thr Thr Glu Asp Ala Asp Lys Tyr Leu Gly Gln
370 375 380
Thr Ser Lys Gln Leu Leu Asp Ser Lys Ile Val Pro Phe Ser Asn Gln
385 390 395 400
His Thr Phe Lys Asn Met Gly Tyr Gln Lys Pro Leu Ala Leu His Lys
405 410 415
Gln Tyr Ala Tyr Arg Ser Asp Asn Ser Ser Ile His Asn Ser Ser Arg
420 425 430
Gly Cys Phe Asn Arg Asp Gly Asp Gly Ala Ser Asp Leu Cys Val Leu
435 440 445
Glu Gly Asn Arg Asn Pro Ala Pro Asp His Arg Leu Pro Tyr Gln Gly
450 455 460
Lys Phe His His Asn Phe Gln Gln His Met Tyr Ser Asn Ser Met Ile
465 470 475 480
Pro Ala Phe Gly Gly Met Arg Tyr Lys Pro His Asp Glu Arg Ile Thr
485 490 495
Leu Arg Leu Ala Leu Gln Asp Ile Ser Gln Pro Lys Ser Glu Ala Asn
500 505 510
Pro Pro Asp Gly Val Leu Ala Val Pro Leu Leu Arg His Gln Lys Ile
515 520 525
Ala Leu Ser Trp Met Val Gln Lys Glu Thr Ser Ser Ser His Cys Ser
530 535 540
Gly Gly Ile Leu Ala Asp Asp Gln Gly Leu Gly Lys Thr Val Ser Ala
545 550 555 560
Ile Ser Leu Ile Leu Thr Glu Arg Ser Pro Val Pro Gln Ser Ser Thr
565 570 575
Ile Lys Asn Glu Pro Cys Glu Ala Val Thr Leu Asp Asp Asp Asp Glu
580 585 590
Asp Asp Ser Val Glu Pro His Pro Lys Lys Leu Met Gln Thr Cys Ser
595 600 605
Ser Lys Val Thr Thr Asn Thr Val Lys Gln Glu Asn Pro Phe Val Ala
610 615 620
Ile Lys Thr Arg Pro Ala Ala Gly Thr Leu Val Val Cys Pro Thr Ser
625 630 635 640
Val Leu Arg Gln Trp Ala Gly Glu Leu Lys Asn Lys Val Thr Ser Lys
645 650 655
Ala Asn Leu Ser Phe Leu Ile Tyr His Gly Ser Asn Arg Thr Lys Asp
660 665 670
Pro Asn Glu Leu Thr Lys Tyr Asp Val Val Leu Thr Thr Tyr Ser Ile
675 680 685
Val Ser Met Glu Val Pro Lys Gln Ser Asn Pro Asp Ser Asp Asp Glu
690 695 700
Glu Lys Gly Lys Pro Asp Arg Tyr Gly Ala Pro Val Ser Ser Ser Gly
705 710 715 720
Ser Lys Lys Arg Lys Ala Pro Ser Lys Lys Thr Lys Cys Lys Ser Ala
725 730 735
Ala Glu Ser Cys Leu Pro Glu Lys Pro Leu Ala Lys Val Ala Trp Phe
740 745 750
Arg Val Ile Leu Asp Glu Ala Gln Ser Ile Lys Asn Tyr Arg Thr Gln
755 760 765
Val Ala Arg Ala Cys Trp Gly Leu Arg Ala Lys Arg Arg Trp Cys Leu
770 775 780
Ser Gly Thr Pro Ile Gln Asn Ala Val Glu Asp Leu Tyr Ser Tyr Phe
785 790 795 800
Arg Phe Leu Arg Tyr Asp Pro Tyr Ala Val Tyr Lys Gln Phe Cys Thr
805 810 815
Met Ile Lys Ile Pro Ile Ser Arg Asn Pro Thr Asn Gly Tyr Lys Lys
820 825 830
Leu Gln Val Val Leu Lys Thr Val Met Leu Arg Arg Thr Lys Ala Thr
835 840 845
Met Leu Asp Gly Lys Pro Ile Ile Ser Leu Pro Pro Lys Thr Val Ser
850 855 860
Leu Lys Thr Val Asp Phe Thr Gly Glu Glu Arg Ala Phe Tyr Asn Thr
865 870 875 880
Leu Glu Val Glu Ser Arg Glu Gln Phe Lys Glu Tyr Ala Ala Ala Gly
885 890 895
Thr Val Lys Gln Asn Tyr Val Asn Ile Leu Leu Met Leu Leu Arg Leu
900 905 910
Arg Gln Ala Cys Asp His Pro His Leu Val Arg Gly Tyr Asn Ser Ser
915 920 925
Ser Ser Trp Met Ser Ser Leu Glu Met Ala Lys Lys Leu Pro Met Glu
930 935 940
Arg Gln His Glu Leu Leu Asn Cys Leu Gln Ser Cys Ser Ala Leu Cys
945 950 955 960
Ala Leu Cys Asn Asp Ala Pro Glu Asp Pro Val Val Thr Ile Cys Gly
965 970 975
His Val Phe Cys Asn Gln Cys Ile Leu Glu Gln Leu Thr Gly Asp Asp
980 985 990
Ser Val Cys Pro Val Ser Asn Cys Arg Val Arg Leu Asn Thr Thr Ser
995 1000 1005
Leu Phe Ser Arg Gly Thr Leu Glu Cys Ser Leu Ser Arg Leu Thr Cys
1010 1015 1020
Asp Phe Lys Ser Asp Asp Asp Thr Cys Met Glu Met Ile His Ala Glu
1025 1030 1035 1040
Lys Arg Pro Gly Ile Asp Ser Ser Tyr Ala Ser Ser Lys Val Arg Ala
1045 1050 1055
Ala Leu Asp Ile Leu Leu Ser Leu Pro Arg Ile Asp Pro Thr Gln Met
1060 1065 1070
Thr Asp Ser Lys Cys Ser Ile Gly Leu Glu Ser Glu Lys Phe Asp Gly
1075 1080 1085
Arg Gly Thr Ser Glu Gln Ile Asp Thr Lys Leu Thr Glu Lys Ala Ile
1090 1095 1100
Val Phe Ser Gln Trp Thr Arg Met Leu Asp Leu Leu Glu Val His Leu
1105 1110 1115 1120
Lys Ala Ser His Val Thr Tyr Arg Arg Leu Asp Gly Thr Met Ser Val
1125 1130 1135
Ala Ala Arg Asp Lys Ala Val Lys Asp Phe Asn Thr Val Pro Glu Val
1140 1145 1150
Thr Val Met Ile Met Ser Leu Lys Ala Ala Ser Leu Gly Leu Asn Met
1155 1160 1165
Val Ala Ala Cys His Val Leu Met Leu Asp Leu Trp Trp Asn Pro Thr
1170 1175 1180
Thr Glu Asp Gln Ala Val Asp Arg Ala His Arg Ile Gly Gln Thr Arg
1185 1190 1195 1200
Pro Val Thr Val Ser Arg Leu Thr Ile Lys Asp Thr Val Glu Asp Arg
1205 1210 1215
Ile Leu Ala Leu Gln Glu Lys Lys Arg Glu Met Val Ala Ser Ala Phe
1220 1225 1230
Gly Glu Asp Lys Ser Gly Ser Arg Gln Thr Arg Leu Thr Val Glu Asp
1235 1240 1245
Leu Asn Tyr Leu Phe Met Val
1250 1255
<210> 132
<211> 1030
<212> PRT
<213> 稻
<400> 132
Met Leu Ala Gly Asn Met Asn Asn Asp Asn Tyr Ile Asp Leu Ser Ser
1 5 10 15
Asp Ser Asp Ile Asp Phe Asp Phe Asp Ser Asp Asp Ser Val Gly Gly
20 25 30
Leu Asp Gln Glu Leu Ala Ser Ser Ser Ser Arg Pro Thr Glu Asn Ile
35 40 45
Asn Gly Gln Tyr Arg Thr Leu Pro Pro Ser Phe Thr Asn Gly Arg His
50 55 60
Val Asp Asn Ala Arg His Ala Leu Gly Ser Gly Asp Arg Ala Tyr Pro
65 70 75 80
His Ser Ser Ser Tyr Arg Gly Ser Pro Asn Asp Ser Ala Arg Ala Thr
85 90 95
Pro Ala Ser Asn Arg Thr Asp Ile Val Val Lys Lys His Asn Gly Phe
100 105 110
Ala Ser Glu Glu Asn Asp Asn Gly Lys Arg Ile Leu Pro Ser Ser Phe
115 120 125
Ser Asn Gly Arg Thr Thr Asn Ala Met His Pro Val Val Ala Ser Glu
130 135 140
Thr Arg Lys Phe Pro Pro Ser Phe Thr Asn Gly Asn Ser Gln Arg Leu
145 150 155 160
Ala Glu Asn Arg Met Gly Lys Asn Val Ala Asn Gly Ile Gly Glu Pro
165 170 175
Ser Ser Ser Arg Phe Pro Ser Arg Ser Ser Phe Gly Thr Asp Asn Lys
180 185 190
Lys Val Ile Thr Asp Ser Asp Asn Glu Asp Val Tyr Val Tyr Gly Ser
195 200 205
Ser Ser Ser His Arg Val Leu Pro Pro Ser Phe Gly Arg Asn Ser Ser
210 215 220
Ala Asn His Ser Glu Phe Ala Asn Gly Ile Asp Met Gln Gly Arg Leu
225 230 235 240
Asn Leu Glu Asn Arg Ile Ile Asp Ser Asp Glu Arg Ala Val Tyr Gln
245 250 255
Glu Ala Leu Gln Asn Ile Ile Gln Asp Lys Arg Glu Asp Asp Leu Pro
260 265 270
Glu Gly Val Leu Ser Val Pro Leu Leu Arg His Gln Lys Met Ala Leu
275 280 285
Ala Trp Met Val Ser Lys Glu Asn Ser Ser His Cys Ala Gly Gly Ile
290 295 300
Leu Ala Asp Asp Gln Gly Leu Gly Lys Thr Val Ser Thr Ile Ala Leu
305 310 315 320
Ile Gln Lys Gln Arg Ile Gln Gln Ser Lys Phe Met Ser Val Asp Ser
325 330 335
Asp Arg Leu Lys Ala Glu Ala Leu Asn Leu Asp Asp Asp Asp Glu Ala
340 345 350
Ala Pro Val Ala Asp Asn Lys Gly Glu Gln Thr Lys Asn Asp Glu Pro
355 360 365
Arg Lys Asp Leu Gly Ala Gly Ser Ser Ser Thr Ala Ala Gly Thr Gly
370 375 380
Asp Val Glu Thr Cys Ala Ser Leu Met Asn Thr Ala Pro Asp Lys Thr
385 390 395 400
Val Glu Arg Asn Val Glu Arg Lys Lys Lys Ser Lys Ala Ser Thr Ser
405 410 415
Ser Thr Met Gln Ser Met Thr Arg Pro Ala Ala Gly Thr Leu Val Val
420 425 430
Cys Pro Ala Ser Val Leu Lys Gln Trp Ala Asn Glu Leu Thr Asp Lys
435 440 445
Val Gly Glu Ser Ala Lys Leu Ser Val Leu Val Tyr His Gly Gly Ser
450 455 460
Arg Thr Lys Asp Pro Asn Glu Leu Ala Lys Tyr Asp Val Val Ile Thr
465 470 475 480
Thr Tyr Thr Ile Val Ala Asn Glu Val Pro Lys Gln Asn Ala Asp Asp
485 490 495
Asp Thr Asp Gln Lys Asn Gly Glu Glu Ser Ser Ala Gly Asn Lys Arg
500 505 510
Lys Gln Pro Pro Lys Ala Gln Ser Lys Ser Lys Lys Lys Lys Lys Lys
515 520 525
Leu Lys Asp Ser Asp Ile Tyr Leu Ala Ser Gly Pro Val Ala Arg Val
530 535 540
Arg Trp Phe Arg Val Val Leu Asp Glu Ala Gln Thr Ile Lys Asn Phe
545 550 555 560
Arg Thr Gln Val Ala Lys Ala Cys Cys Gly Leu Arg Ala Lys Arg Arg
565 570 575
Trp Cys Leu Ser Gly Thr Pro Ile Gln Asn Ala Ile Asp Glu Leu Tyr
580 585 590
Ser Tyr Phe His Phe Leu Lys Tyr Asp Pro Tyr Ser Thr Tyr Asn Ser
595 600 605
Phe Cys Thr Met Ile Lys His Pro Ile Ala Arg Asn Ala Val His Gly
610 615 620
Tyr Lys Lys Leu Gln Thr Val Leu Arg Ile Val Leu Leu Arg Arg Thr
625 630 635 640
Lys Glu Thr Leu Ile Asp Gly Glu Pro Ile Ile Lys Leu Pro Pro Lys
645 650 655
Thr Ile Asn Leu Asp Lys Val Asp Phe Thr Lys Glu Glu Arg Ala Phe
660 665 670
Tyr Leu Thr Leu Glu Glu Arg Ser Arg Gln Gln Phe Lys Ala Tyr Ala
675 680 685
Ala Ala Gly Thr Leu Lys Gln Asn Tyr Ala Asn Ile Leu Leu Met Leu
690 695 700
Leu Arg Leu Arg Gln Ala Cys Asp His Pro Leu Leu Val Lys Gly His
705 710 715 720
Gln Ser Glu Tyr Lys Gly Asp Gly Ser Ile Glu Met Ala Lys Gln Leu
725 730 735
Pro Lys Glu Met Ile Ile Asn Leu Leu Ala Lys Leu Glu Val Gly Glu
740 745 750
Phe Cys Ser Val Cys Ser Asp Val Pro Glu Asp Ala Val Val Thr Met
755 760 765
Cys Gly His Val Phe Cys Tyr Gln Cys Ile Tyr Glu Arg Ile Thr Thr
770 775 780
Asp Glu Asn Met Cys Pro Ser Pro Asn Cys Gly Asn Thr Leu Ser Thr
785 790 795 800
Asp Ser Val Phe Ser Ser Gly Ala Leu Arg Ile Cys Met Ser Gly Val
805 810 815
Ser Ser Ser His Ala Ser Gly Ser Ser Ser Leu Asp Asp Glu Ser Ser
820 825 830
Ser Ile Ser Gln Thr Ser Tyr Ile Ser Ser Lys Ile Gln Ala Ala Ile
835 840 845
Asp Ile Leu Asn Ser Ile Ile Asn Thr Tyr Ala Leu Thr Asp Ser Asp
850 855 860
Thr Val Glu Ser Asn Pro Ser Arg Val Ala Pro Val Lys Ala Ile Val
865 870 875 880
Phe Ser Gln Trp Thr Gly Met Leu Asp Leu Leu Glu Leu Ser Leu Asn
885 890 895
Ser Asn Leu Ile Gln Tyr Arg Arg Leu Asp Gly Thr Met Ser Leu Asn
900 905 910
Ser Arg Asp Lys Ala Val Lys Asp Phe Asn Thr Asp Pro Glu Val Arg
915 920 925
Val Met Ile Met Ser Leu Lys Ala Gly Asn Leu Gly Leu Asn Met Val
930 935 940
Ala Ala Cys His Val Ile Leu Leu Asp Leu Trp Trp Asn Pro Tyr Ala
945 950 955 960
Glu Asp Gln Ala Ile Asp Arg Ala His Arg Ile Gly Gln Thr Arg Pro
965 970 975
Val Thr Val Ser Arg Leu Thr Ile Lys Asp Thr Val Glu Asp Arg Ile
980 985 990
Leu Ala Leu Gln Glu Glu Lys Arg Ala Met Val Ser Ser Ala Phe Gly
995 1000 1005
Glu Asp Lys Ser Gly Gly His Ala Thr Arg Leu Thr Val Asp Asp Leu
1010 1015 1020
Lys Tyr Leu Phe Arg Phe
1025 1030
<210> 133
<211> 1070
<212> PRT
<213> 二穗短柄草
<400> 133
Met Leu Ala Ser Asn Met Asn Asn Asp Asn Asp Asn Val Ile Asp Leu
1 5 10 15
Ile Ser Asp Ser Asp Asp Asp Phe Asp Phe Asp Ser Asp Asp Pro Thr
20 25 30
Ser Thr Ser Val Thr Ser Val Arg Asn Gly Glu Gly Gln Pro Ile Arg
35 40 45
Leu Gln Asp Glu Asp Leu Ala Arg Thr Met Leu Ser Thr Pro Ser Ser
50 55 60
Ser Arg Pro Leu Lys Asn Ser Asn Gly Gln Tyr Arg Thr Leu Pro Pro
65 70 75 80
Ser Phe Ala Asn Gly Ile Glu Pro Leu Lys Asn Ser Asn Gly Gln Tyr
85 90 95
Arg Thr Leu Pro Pro Ser Phe Ala Asn Gly Ile Asp Ile Lys Lys Ala
100 105 110
Arg Tyr Thr Arg Gly Ser Gly Asp Arg Thr Tyr Pro His Ser Ser Ser
115 120 125
Tyr Val Gly Ser Pro Tyr Asp Ser Ala Arg Ala Thr Leu Thr Ser Asn
130 135 140
Arg Ile Ala Ser Val Val Lys Glu Leu Asn Gly Ser Ala Leu Asp Ala
145 150 155 160
Asn Asp Asn Asp Lys Met Ile Leu Pro Ser Ser Phe Ser Asn Gly Ser
165 170 175
Thr Ala Lys Ser Ile Asn Pro Asn Val Ala Ser Asp Ser Arg Arg Leu
180 185 190
Pro Ser Arg Phe Ala Asn Gly Asn Ser Gln Lys Met Val Glu Asn Met
195 200 205
Met Glu Ser Asn Asp Ala Lys Gln Ile Gly Gln Pro Ser Ser Ser Lys
210 215 220
Phe Pro Thr Gln Asn Leu Ser Leu Ser Asn Ala Gln Lys Val Ile Thr
225 230 235 240
Asp Asp Asp Asp Asp Asp Asp Ile Tyr Val Tyr Gly Gly Thr Ser Ser
245 250 255
Asn Arg Val Leu Pro Ser Ser Phe Gly Gly Asn Asn Ser Ala Asn Asn
260 265 270
Thr Asp Leu Val Asn Gly Asn Gly Met Gln Ala Arg Pro Asn Leu Glu
275 280 285
Ser Arg Phe Leu Asp Ser Glu Glu Arg Ala Val Tyr Gln Glu Ala Leu
290 295 300
Gln Asn Ile Ser Leu Asp Lys Lys Glu Asp Asp Leu Pro Glu Gly Tyr
305 310 315 320
Leu Ser Val Ser Leu Leu Lys His Gln Lys Met Ala Leu Ala Trp Met
325 330 335
Val Ser Lys Glu Asn Ser Ser His Cys Ala Gly Gly Ile Leu Ala Asp
340 345 350
Asp Gln Gly Leu Gly Lys Thr Val Ser Thr Ile Ala Leu Ile Gln Lys
355 360 365
Gln Arg Ile Pro Gln Ser Lys Phe Met Ser Ala Asp Ser Asp Ala Leu
370 375 380
Lys Ser Glu Ala Leu Asn Leu Asp Glu Asp Asp Glu Thr Val Thr Val
385 390 395 400
Val Asp Lys Gly Glu Gln Ile Leu Asn Asn Glu Pro Lys Glu Leu Gly
405 410 415
Ala Ser Leu Ser Ser Thr Ala Ala Ser Ile Ser Gly Val Lys Pro Cys
420 425 430
Ile Ser Glu Ile Glu Val Pro Asp Arg Met Ala Glu Ser Lys Val Glu
435 440 445
Cys Lys Lys Lys Thr Lys Thr Gly Thr Ser Ser Ala Ser Ser Met His
450 455 460
Ser Met Thr Arg Pro Ala Ala Gly Thr Leu Val Val Cys Pro Ala Ser
465 470 475 480
Val Leu Lys Gln Trp Ala Asn Glu Leu Thr Asp Lys Val Gly Glu Ser
485 490 495
Ala Lys Leu Ser Val Leu Val Tyr His Gly Gly Ala Arg Thr Lys Asn
500 505 510
Pro Ser Glu Leu Ala Lys Tyr Asp Val Val Val Thr Thr Tyr Thr Ile
515 520 525
Val Ala Asn Glu Val Pro Lys Gln Asn Ala Asp Asp Asp Pro Asp Gln
530 535 540
Lys Asn Ala Glu Glu Ser Ser Ala Gly Asn Lys Thr Lys Pro Ser Ser
545 550 555 560
Lys Ser Lys Lys Arg Lys Lys Lys Leu Lys Asp Ser Asp Ile Asp Leu
565 570 575
Asn Ser Gly Pro Val Ala Arg Val Arg Trp Phe Arg Val Val Leu Asp
580 585 590
Glu Ala Gln Thr Ile Lys Asn Phe Arg Thr Gln Val Ala Lys Ala Cys
595 600 605
Cys Gly Leu Arg Ala Lys Arg Arg Trp Cys Leu Ser Gly Thr Pro Ile
610 615 620
Gln Asn Ala Ile Asp Glu Leu Tyr Ser Tyr Phe Arg Phe Leu Lys Tyr
625 630 635 640
Asp Pro Tyr Ser Thr Tyr Ser Ser Phe Cys Thr Met Ile Lys His Pro
645 650 655
Ile Ala Arg Asp Ala Val His Gly Tyr Lys Lys Leu Gln Thr Val Leu
660 665 670
Arg Ile Val Leu Leu Arg Arg Thr Lys Glu Thr Lys Ile Asn Gly Glu
675 680 685
Pro Ile Ile Asn Leu Pro Pro Lys Thr Ile Asn Leu Asn Lys Val Asp
690 695 700
Phe Thr Lys Glu Glu Arg Ala Phe Tyr Ser Thr Leu Glu Glu Arg Ser
705 710 715 720
Gln Gln Gln Phe Lys Glu Tyr Ala Ala Ala Gly Thr Leu Asn Gln Asn
725 730 735
Tyr Ala Asn Ile Leu Leu Leu Leu Leu Arg Leu Arg Gln Ala Cys Asp
740 745 750
His Pro Leu Leu Val Lys Gly His Gln Ser Val Phe Lys Gly Asp Gly
755 760 765
Ser Ile Glu Arg Ala Arg Gln Leu Pro Lys Asp Leu Val Ile Asp Leu
770 775 780
Leu Ala Lys Leu Glu Val Ser Ser Leu Cys Ala Val Cys Arg Asp Thr
785 790 795 800
Pro Glu Asp Ala Val Val Ala Met Cys Gly His Ile Phe Cys Tyr Gln
805 810 815
Cys Ile His Glu Arg Ile Thr Thr Asp Glu Asn Met Cys Pro Thr Pro
820 825 830
Asn Cys Arg Thr Thr Leu Ser Thr Glu Ser Val Phe Ser Ser Gly Thr
835 840 845
Leu Arg Ile Cys Ile Ala Gly Asn Thr Cys Thr Tyr Ala Thr Ala Ser
850 855 860
Ser Ser Ala Asp Asp Glu Leu Ser Ser Ile Ser Gln Ser Ser Tyr Met
865 870 875 880
Ser Ser Lys Ile Arg Ala Thr Val Asp Glu Leu Asn Thr Ile Ile Asn
885 890 895
Thr His Ala Ile Thr Asp Ser Asp Thr Ser Glu Ser Asn Pro Ser Gln
900 905 910
Val Ala Pro Val Lys Ala Ile Val Phe Ser Gln Trp Thr Gly Met Leu
915 920 925
Asp Gln Leu Glu Leu Ser Leu Asn Asn Asn Leu Ile Arg Tyr Arg Arg
930 935 940
Leu Asp Gly Thr Met Ser Leu Asn Leu Arg Asp Arg Ala Val Lys Asp
945 950 955 960
Phe Asn Thr Asp Pro Glu Val Arg Val Met Ile Met Ser Leu Lys Ala
965 970 975
Gly Asn Leu Gly Leu Asn Met Val Ala Ala Cys His Val Ile Leu Leu
980 985 990
Asp Val Trp Trp Asn Pro Tyr Ala Glu Asp Gln Ala Ile Asp Arg Ala
995 1000 1005
His Arg Ile Gly Gln Thr Arg Ala Val Thr Val Ser Arg Leu Thr Ile
1010 1015 1020
Lys Asp Thr Val Glu Asp Arg Ile Leu Ala Leu Gln Glu Glu Lys Arg
1025 1030 1035 1040
Ala Met Val Asn Ser Ala Phe Gly Glu Asp Lys Ser Gly Gly His Ala
1045 1050 1055
Thr Arg Leu Thr Val Glu Asp Leu Arg Tyr Leu Phe Arg Ile
1060 1065 1070
<210> 134
<211> 1327
<212> PRT
<213> 毛果杨
<400> 134
Met Ile Met Ala Asp Gly Arg Ser Gly Ser Val Met Pro Gly Gly Glu
1 5 10 15
Ile Met Ala Val Gly Asp Gly Asn Ser Gly Asp Gly Glu Glu Glu Asp
20 25 30
Leu Ser Met Asp Val Asp Leu Phe Tyr Thr Ile Leu Gly Glu Glu Pro
35 40 45
Ser Pro Ala Ser Pro Lys Ile Glu Gly Ala Asp Gly Leu Gly Gly Glu
50 55 60
Ser Met Asp His Ala Ala Tyr Ser Leu His Asn Pro Glu Gly Ser Asp
65 70 75 80
Ala Arg Ala Gly His Leu Gly Gly Ser Ile Asp Tyr Thr Gly Arg Gln
85 90 95
Met Ser Ser Leu Met His Ala Arg Ser Gly Ser Ser Arg Glu Phe Cys
100 105 110
Phe Pro Phe Gln Lys Asp Gln Gly Thr Met Arg Ala Gly Ala Ser Gln
115 120 125
Ser Glu Ile Ala Ser Cys Ile Thr Glu Pro Thr Thr Phe Ala Asp Gly
130 135 140
Val Ser Ser Cys Val Ala Asp His Ala Gly Ser Ser His Gly Pro Arg
145 150 155 160
Met Asp Gly Leu Asp Glu Lys Phe Gly Ser Arg Asp Ala Leu Asp Asn
165 170 175
Asp Ser Leu Gly Ile Leu Glu Leu Lys Thr Asp Ile His Arg Ser Met
180 185 190
Val Met Pro Leu Met Asp Thr Asp His Asp Met Ile Ser Thr Lys Ser
195 200 205
Ala Asp Trp His Tyr Pro Gly Phe Asn Ser Glu Leu Arg Asp His Asp
210 215 220
Ser Ala Met Gln Phe Gly Met Asn Gly Tyr Asp Ala His Tyr Thr Asp
225 230 235 240
Ser Ser Gly Phe Asp Phe Ser Ser Asp Phe Asn Phe Gly Leu Phe Pro
245 250 255
Ile Asn Gln Glu Ile Asn Glu Phe Gln Pro Glu Asn Ala Cys Ser Gly
260 265 270
Pro Glu Ile Ser Met Met Pro Cys Ser Asp Val Asn Gly Met Asn Phe
275 280 285
Lys Ser Glu Gly Asp Gly Tyr Thr Phe Pro Lys Thr Arg Lys Phe Ser
290 295 300
Ser Ser Ala Asp Asp Gly Leu Asn His Asp Lys Ala Ser Val Met Pro
305 310 315 320
Pro Ser Asp Ile Gln Leu Gly Ile Ser Glu Val Gln Thr Val Cys Val
325 330 335
Glu Asp Glu Lys Thr Asp Gly Leu Val Ala Cys Arg Asn Met Thr Trp
340 345 350
Gln Thr Gly Glu Gly Val Thr Glu Ala Val Asp Arg Lys Cys Ser Trp
355 360 365
Ser Asp Gly Asn Gly Thr Phe Val Tyr Lys Asp Lys Gln Gln Ser Pro
370 375 380
Ser Gly Val Leu Ser Ser Val Gln Ser Gln Lys His Val Ile Tyr Thr
385 390 395 400
Asn Asp Asp Arg Gly Gly Met Ala Leu Gly Ser Ser Arg Ala Gln Val
405 410 415
Glu Gly Ile Ala Gly Arg Phe Pro Phe Asp Ser Val Tyr Leu Asn Leu
420 425 430
Ser Ala Ser Glu Gln Tyr Leu Pro Phe Ala Pro Thr Ser His Leu Ser
435 440 445
Lys Met Gln Leu Gly Cys Gly Lys Asp Glu Lys Gln Gly Leu Pro Ile
450 455 460
His Ser Lys Ala Leu Gly Ser His Leu Ser Ile Val Ser Pro Glu Ser
465 470 475 480
Ile Gln Ser Asn Ser Ser Gly Ser Lys Ser His Val Asp Asp Glu Pro
485 490 495
Asp Ile Cys Ile Leu Asp Asp Ile Ser Gln Pro Ala Arg Ser Asn Gln
500 505 510
Cys Phe Ala Pro Ser Lys Pro Ile Val Pro Leu Leu His Pro Thr Tyr
515 520 525
Asn Asp Ser Leu His His Ser Thr Val Glu Gly Thr Arg Phe Lys Ala
530 535 540
Asn Asp Glu Gln Leu Val Leu Arg Val Ala Leu Gln Asp Leu Ala Gln
545 550 555 560
Pro Lys Ser Glu Ala Val Pro Pro Asp Gly Phe Leu Ala Val Pro Leu
565 570 575
Leu Arg His Gln Arg Ile Ala Leu Ser Trp Met Val Gln Lys Glu Thr
580 585 590
Ser Ser Leu His Cys Ser Gly Gly Ile Leu Ala Asp Asp Gln Gly Leu
595 600 605
Gly Lys Thr Val Ser Thr Ile Ala Leu Ile Leu Lys Glu Arg Ala Pro
610 615 620
Leu Cys Arg Val Asp Ala Val Ala Val Lys Lys Glu Glu Cys Glu Thr
625 630 635 640
Leu Asn Leu Asp Asp Asp Asp Asp Gly Val Ile Glu Ile Asp Arg Leu
645 650 655
Lys Lys Gly Ala Asp Gly Ser Gln Val Lys Ser Asn Arg Ser Ser Thr
660 665 670
Lys Ser Leu Asn Ser Pro Gly Gln Ser Lys Gly Arg Pro Ala Ala Gly
675 680 685
Thr Leu Ile Val Cys Pro Thr Ser Val Leu Arg Gln Trp Ala Asp Glu
690 695 700
Leu His Thr Lys Val Thr Thr Glu Ala Asn Leu Ser Val Leu Val Tyr
705 710 715 720
His Gly Ser Asn Arg Thr Lys Asp Pro Ser Glu Val Ala Lys Tyr Asp
725 730 735
Val Val Val Thr Thr Tyr Ser Ile Val Ser Met Glu Val Pro Lys Gln
740 745 750
Pro Leu Ala Asp Glu Asp Glu Glu Lys Gln Arg Met Glu Gly Asp Asp
755 760 765
Val Pro His Leu Gly Leu Ser Tyr Gly Lys Lys Arg Lys Tyr Pro Pro
770 775 780
Thr Ser Gly Lys Lys Gly Leu Lys Asn Lys Lys Gly Met Asp Ser Ala
785 790 795 800
Met Leu Glu Ser Ile Ala Arg Pro Leu Ala Lys Val Ala Trp Phe Arg
805 810 815
Val Val Leu Asp Glu Ala Gln Ser Ile Lys Asn His Arg Thr Gln Val
820 825 830
Ala Arg Ala Cys Trp Gly Leu Arg Ala Lys Arg Arg Trp Cys Leu Ser
835 840 845
Gly Thr Pro Ile Gln Asn Ala Ile Asp Asp Leu Tyr Ser Tyr Phe Arg
850 855 860
Phe Leu Arg Tyr Glu Pro Tyr Ala Val Tyr Lys Leu Phe Cys Ser Ala
865 870 875 880
Ile Lys Val Pro Ile Gln Lys Asn Pro Ala Lys Gly Tyr Arg Lys Leu
885 890 895
Gln Ala Val Leu Lys Thr Val Met Leu Arg Arg Thr Lys Gly Thr Leu
900 905 910
Leu Asp Gly Glu Pro Ile Ile Asn Leu Pro Pro Lys Val Val Glu Leu
915 920 925
Lys Lys Val Asp Phe Thr Glu Glu Glu Arg Asp Phe Tyr Thr Arg Leu
930 935 940
Glu Ile Asp Ser Arg Ala Gln Phe Lys Glu Tyr Ala Ala Ala Gly Thr
945 950 955 960
Val Lys Gln Asn Tyr Val Asn Ile Leu Leu Met Leu Leu Arg Leu Arg
965 970 975
Gln Ala Cys Asp His Pro Leu Leu Val Lys Gly Leu Asp Ser Asn Ser
980 985 990
Leu Gly Gly Ser Ser Ile Glu Met Ala Lys Lys Leu Pro Gln Glu Lys
995 1000 1005
Gln Leu Cys Leu Leu Lys Cys Leu Glu Ala Ser Leu Ala Ile Cys Gly
1010 1015 1020
Ile Cys Ser Asp Pro Pro Glu Asp Ala Val Val Ser Val Cys Gly His
1025 1030 1035 1040
Val Phe Cys Lys Gln Cys Ile Cys Glu His Leu Thr Gly Asp Asp Asn
1045 1050 1055
Gln Cys Pro Val Ser Asn Cys Lys Val Arg Leu Asn Val Ser Ser Val
1060 1065 1070
Phe Ser Lys Ala Thr Leu Asn Ser Ser Leu Ser Asp Glu Pro Asp Gln
1075 1080 1085
Asp Ser Ser Gly Ser Glu Leu Val Ala Ala Val Ser Ser Ser Ser Asp
1090 1095 1100
Asn Arg Pro His Asn Ser Ser Lys Ile Arg Ala Thr Leu Glu Val Leu
1105 1110 1115 1120
Gln Ser Leu Thr Lys Pro Lys Asp Cys Leu Ser Lys Cys Asn Leu Ser
1125 1130 1135
Glu Asn Ser Ala Asp Gly Asn Val Ala Cys His Glu Thr Ser Ser Gly
1140 1145 1150
Ser Thr Gly Ser Leu Asn Asp Gly Thr Asp Lys Arg His Pro Pro Ala
1155 1160 1165
Lys Val Val Gly Glu Lys Ala Ile Val Phe Ser Gln Trp Thr Gly Met
1170 1175 1180
Leu Asp Leu Leu Glu Ala Cys Leu Lys Ser Ser Ser Ile Gln Tyr Arg
1185 1190 1195 1200
Arg Leu Asp Gly Thr Met Ser Val Val Ala Arg Asp Lys Ala Val Lys
1205 1210 1215
Asp Phe Asn Thr Leu Pro Glu Val Ser Val Met Ile Met Ser Leu Lys
1220 1225 1230
Ala Ala Ser Leu Gly Leu Asn Met Val Ala Ala Cys His Val Leu Leu
1235 1240 1245
Leu Asp Leu Trp Trp Asn Pro Thr Thr Glu Asp Gln Ala Ile Asp Arg
1250 1255 1260
Ala His Arg Ile Gly Gln Thr Arg Lys Val Thr Val Leu Arg Leu Thr
1265 1270 1275 1280
Val Lys Asn Thr Val Glu Asp Arg Ile Leu Ala Leu Gln Gln Lys Lys
1285 1290 1295
Arg Glu Met Val Ala Ser Ala Phe Gly Glu Asp Glu Asn Gly Gly Arg
1300 1305 1310
Gln Thr Arg Leu Thr Val Asp Asp Leu Asn Tyr Leu Phe Met Val
1315 1320 1325
<210> 135
<211> 1013
<212> PRT
<213> 葡萄
<400> 135
Met Asp Pro Ile Asp Ile Thr Ser Ser Ser Asp Ser Asp Phe Asp Phe
1 5 10 15
Glu Asp Asp Arg Glu Thr Asp Thr Ser Pro Val Gly Glu Ser Val Ala
20 25 30
Phe Ala Asn Ser Arg Ile Leu Pro Pro Trp Pro Ser Thr Ser Gly Thr
35 40 45
Asn Ser Lys Ser Thr Ser His Gly His Phe Gln Lys Val Pro Ser Pro
50 55 60
Lys Arg Ala Ser Ala Ser Asn Gly Ser Ser Ser Asn Phe Tyr His Tyr
65 70 75 80
Pro Pro Lys Ile Gln Met His Pro Ser Phe Asp Asp Asp Ile Arg Ala
85 90 95
Ser Asn Arg His Asn Phe Arg Glu Ala Asp Phe Asn Tyr Ser Thr Glu
100 105 110
Asn Gly Asp Met Leu Asp Val Glu Asn His Gln Gln Leu Ile Asn Leu
115 120 125
Asn Lys Ala Asp Ile Phe Gly Ala Asp Tyr Glu Lys Leu Ser Gln Pro
130 135 140
Ala Met Arg Arg Thr Leu Pro Ser Thr Leu Gln Pro Ser Ala Pro Ser
145 150 155 160
Ala Gly Met Asn Asn Thr Val Gly Asn Ile Gly Ser Ser His Ile His
165 170 175
Asp Ser Gln Gly Lys Ser Phe His Pro Val Gly Pro Ile Leu Asn Asn
180 185 190
Met Asn Tyr Met Lys Glu His Phe Gly Arg Gly Asn Asp Asp Glu Val
195 200 205
Ile Met Tyr Glu Asn Ser Gly Ser Arg Ile Leu Pro Pro Ser Leu Met
210 215 220
His Gly Lys Ser Val Pro Ser Thr Gln Tyr Gly Gly Val Ser Glu Ser
225 230 235 240
Ala Tyr Arg Pro Gly Val Ala Glu Glu Met Ala Ala Asn Thr Asp Glu
245 250 255
Arg Leu Val Tyr Gln Ala Ala Leu Gln Asp Leu Asn Gln Pro Lys Val
260 265 270
Glu Ala Thr Leu Pro Asp Gly Leu Leu Thr Val Ser Leu Leu Arg His
275 280 285
Gln Lys Ile Ala Leu Ala Trp Met His Gln Lys Glu Thr Arg Ser Leu
290 295 300
His Cys Leu Gly Gly Ile Leu Ala Asp Asp Gln Gly Leu Gly Lys Thr
305 310 315 320
Val Ser Met Ile Ala Leu Ile Gln Met Gln Lys Ser Leu Gln Ser Lys
325 330 335
Ser Lys Ser Glu Glu Leu His Asn His Ser Thr Glu Ala Leu Asn Leu
340 345 350
Asp Asp Asp Asp Asp Asn Ala Asn Ala Ala Gly Ser Asp Lys Gly Lys
355 360 365
Gln Thr Glu Glu Thr Ser Asp Ser Lys Pro Ile Ser Glu Val Ser Ala
370 375 380
Ser Leu Pro Glu Phe Arg Arg Arg Arg Pro Ala Ala Gly Thr Leu Val
385 390 395 400
Val Cys Pro Ala Ser Val Leu Arg Gln Trp Ala Arg Glu Leu Asp Glu
405 410 415
Lys Val Ser Glu Glu Ala Lys Leu Ser Val Cys Leu Tyr His Gly Gly
420 425 430
Ser Arg Thr Lys Asp Pro Val Glu Leu Ala Lys Tyr Asp Val Val Leu
435 440 445
Thr Thr Tyr Ser Ile Val Thr Asn Glu Val Pro Lys Gln Pro Leu Val
450 455 460
Asp Asp Asp Glu Gly Asp Glu Arg Asn Gly Glu Lys Tyr Gly Leu Ser
465 470 475 480
Ser Glu Phe Ser Val Asn Lys Lys Arg Lys Lys Pro Ser Asn Val Ser
485 490 495
Lys Arg Gly Lys Lys Gly Arg Lys Gly Ile Asp Ser Ser Ser Ile Asp
500 505 510
Tyr Asp Cys Gly Pro Leu Ala Arg Val Gly Trp Phe Arg Val Ile Leu
515 520 525
Asp Glu Ala Gln Thr Ile Lys Asn His Arg Thr Gln Val Ala Arg Ala
530 535 540
Cys Cys Ser Leu Arg Ala Lys Arg Arg Trp Cys Leu Ser Gly Thr Pro
545 550 555 560
Ile Gln Asn Ala Ile Asp Asp Leu Tyr Ser Tyr Phe Arg Phe Leu Lys
565 570 575
Tyr Asp Pro Tyr Ala Val Tyr Lys Ser Phe Tyr Asn Thr Ile Lys Val
580 585 590
Pro Ile Ser Arg Asn Ser Val His Gly Tyr Lys Lys Leu Gln Ala Val
595 600 605
Leu Arg Ala Ile Met Leu Arg Arg Thr Lys Gly Thr Leu Ile Asp Gly
610 615 620
Thr Pro Ile Ile Asn Leu Pro Pro Lys Thr Ile Cys Leu Ser Lys Val
625 630 635 640
Asp Phe Ser Ser Glu Glu Arg Ala Phe Tyr Ser Lys Leu Glu Ala Asp
645 650 655
Ser Arg Ser Gln Phe Lys Glu Tyr Ala Ala Ala Gly Thr Val Asn Gln
660 665 670
Asn Tyr Ala Asn Ile Leu Leu Met Leu Leu Arg Leu Arg Gln Ala Cys
675 680 685
Asp His Pro Leu Leu Val Lys Gly Tyr Asn Thr Asp Ser Ile Arg Lys
690 695 700
Val Ser Ser Glu Met Ala Lys Lys Leu Pro Ser Asp Ile Leu Ile Asn
705 710 715 720
Leu Leu Asp Ile Leu Glu Thr Ser Ala Ile Cys Arg Val Cys Asn Asp
725 730 735
Pro Pro Glu Asp Ala Val Val Thr Met Cys Gly His Val Phe Cys Tyr
740 745 750
Gln Cys Val Ser Glu Tyr Leu Thr Gly Asp Asp Asn Thr Cys Pro Ala
755 760 765
Leu Glu Cys Lys Glu Gln Leu Gly Ala Asp Val Val Phe Ser Lys Ala
770 775 780
Thr Leu Ile Ser Cys Ile Ser Asp Glu Leu Asp Gly Ser Leu Ser Asn
785 790 795 800
Ser Ser Gln Ser Ala Glu Lys Ser Ile Asn Leu Gln Asn Glu Tyr Ser
805 810 815
Ser Ser Lys Ile Arg Ala Ala Leu Glu Ile Leu Gln Ser His Cys Lys
820 825 830
Leu Thr Ser Pro Asp Ser Asp Pro His Ser Ser Met Gly Cys Asn Gly
835 840 845
Ser Tyr Ser Asn Pro Glu Thr Glu Gly Pro Ile Lys Ala Ile Val Phe
850 855 860
Ser Gln Trp Thr Ser Met Leu Asp Leu Val Glu Met Ser Met Asn His
865 870 875 880
Ser Cys Ile Gln Tyr Arg Arg Leu Asp Gly Thr Met Ser Leu Ala Ser
885 890 895
Arg Asp Arg Ala Val Lys Asp Phe Asn Thr Asp Pro Glu Val Thr Val
900 905 910
Met Leu Met Ser Leu Lys Ala Gly Asn Leu Gly Leu Asn Met Val Ala
915 920 925
Ala Ser Leu Val Ile Leu Leu Asp Leu Trp Trp Asn Pro Thr Thr Glu
930 935 940
Asp Gln Ala Val Asp Arg Ala His Arg Ile Gly Gln Thr Arg Pro Val
945 950 955 960
Thr Val Ser Arg Ile Thr Ile Lys Asp Thr Val Glu Asp Arg Ile Leu
965 970 975
Ala Leu Gln Glu Asp Lys Arg Lys Met Val Ala Ser Ala Phe Gly Glu
980 985 990
Asp Gln Thr Gly Gly Ser Ala Thr Arg Leu Thr Val Glu Asp Leu Lys
995 1000 1005
Tyr Leu Phe Met Val
1010
<210> 136
<211> 1286
<212> PRT
<213> 黄瓜
<400> 136
Met Val Ile Ser Asp Asp Leu Ile Asn Gln Ser Pro Leu Leu Ser Ile
1 5 10 15
Ala Gly Glu Val Leu Gly Phe Gln Thr Phe Leu Ile Arg Phe Cys Phe
20 25 30
Gln Thr Ser Leu Phe Ala Gly Leu Cys Val Phe Ser Gly Phe Ser Leu
35 40 45
Met Ile Ile Leu Met Ala Asp Glu Ala Ser Asn Phe Pro Leu Gln Tyr
50 55 60
Ala Asp Asp Asp Phe Asp Glu Asp Met Ser Met Glu Tyr Glu Lys Ile
65 70 75 80
Leu His Leu Leu Ser Glu Asp Leu Asp Pro Leu Gln Ile Lys Thr Glu
85 90 95
Asp Leu Ser Pro Asn Asn Ala Ser Thr Gly Gln Pro Ala Phe Asp Ser
100 105 110
Ser Asn Gln Glu Asn Phe Gln Leu Gln Asn Asp Ile Ser His Gly Phe
115 120 125
Met Asp Val Thr Leu Lys Asn His Asp Ser Leu Asp Gly Lys Gly Thr
130 135 140
Glu Thr Leu Arg Ser Ser Glu Asn Asn Ser Cys Ala Ser Val Glu Leu
145 150 155 160
Pro Ser Phe Asp Ala Glu His Ser Ser Lys Glu Val Phe Pro Thr Glu
165 170 175
Ser Thr Val Asn Pro Ser Phe Asp Phe Val Thr Asp Val Thr Asn Ser
180 185 190
Tyr Ser Thr Met Pro Tyr Trp Met Ser Thr Val Glu Gln Pro Phe Leu
195 200 205
Val Ser Ser Gln Tyr Leu Phe Pro Gly Asp Tyr Asp Ser Pro Leu Val
210 215 220
Ser Gly Asn Gly Asp Met Thr Val Asn Met Met His Asp Val Glu Phe
225 230 235 240
Pro Ser Asn Ser Leu Cys Ser Ser Thr Thr Met Asp Leu Tyr Ala Gln
245 250 255
Gly Ala Thr Asp His Lys Ser Val Ser Arg Glu Ser Val Ser Lys Asp
260 265 270
Leu Ile Leu Asp Arg Tyr Ser Asn Val Lys Arg Trp Asp Gln Asn Cys
275 280 285
Glu Ser Gly Asn Phe Ile Ser Ser Phe Asp Gly Lys Tyr Pro Phe His
290 295 300
Val Asp Asn Leu His Ile Gly Gln Ala Ser Met Gly Ile Pro Met Ser
305 310 315 320
Thr Glu Leu Asn Ser Ser Cys Lys Glu Leu Val Ser Gln Met Lys Asn
325 330 335
Glu Thr Met Asp Ser Leu Val Glu Ser Cys Ser Gly Pro Trp Gln Ser
340 345 350
Met Met Glu Glu Asn Leu Phe Phe Gln Ser Gln Arg Val Phe Arg Ser
355 360 365
Glu Asp Met Val Cys Gly Thr Ser Gly Arg Leu Ser Asn Asp Gly Arg
370 375 380
Tyr Gln Asn Leu Tyr Ile Thr Asp Gln Tyr Ser Pro Asn Gly His Ser
385 390 395 400
Ser Asn Leu Ser Asn Gln Pro Leu Val Phe Ile Lys Asp Asp Arg Asp
405 410 415
His Lys Leu Ser Val Arg Lys Ser Asp Ile Asp His Pro Gln Val Ser
420 425 430
Pro Glu Ser Thr His Ser Asn Leu Ser Asp Arg Ala His Val Glu Asp
435 440 445
Asp Pro Asp Ile Cys Ile Ile Glu Asp Met Ser His Pro Ala Pro Ser
450 455 460
Asn Arg Ser Leu Met Val Gly Lys Ser Val Ala Ser Gln Ser Phe Ser
465 470 475 480
Ile Val Ser Gly Ser Ser Thr Tyr Met Gly Ile Gly Ser Leu Arg Gln
485 490 495
Lys Ala Lys Asp Ile Asp Ile Leu Lys Val Ala Leu Gln Asp Leu Ser
500 505 510
Gln Pro Lys Ser Glu Thr Ser Pro Pro Asp Gly Ala Leu Asp Val Pro
515 520 525
Leu Leu Arg His Gln Arg Ile Ala Leu Ser Trp Met Val Gln Lys Glu
530 535 540
Thr Ser Ser Val Pro Cys Ala Gly Gly Ile Leu Ala Asp Asp Gln Gly
545 550 555 560
Leu Gly Lys Thr Ile Ser Thr Ile Ala Leu Ile Leu Lys Glu Arg Ala
565 570 575
Pro Ile Arg Ala Cys Pro Thr Val Lys His Glu Glu Leu Glu Thr Leu
580 585 590
Asn Leu Asp Glu Asp Asp Asp Ile His Pro Glu His Asp Gly Pro Lys
595 600 605
Gln Glu Phe Ser His Gln Val Ser Pro Ser Lys Asp Leu Thr Leu Ser
610 615 620
Lys Asn Thr Ser Val Gln Ala Lys Gly Arg Pro Ala Ala Gly Thr Leu
625 630 635 640
Val Val Cys Pro Thr Ser Val Leu Arg Gln Trp Ala Asp Glu Leu His
645 650 655
Asn Lys Val Ser Ser Lys Ala Asn Leu Ser Val Leu Val Tyr His Gly
660 665 670
Ser Ser Arg Thr Lys Asp Pro Cys Glu Leu Ala Lys Tyr Asp Val Val
675 680 685
Leu Thr Thr Tyr Ser Ile Val Ser Met Glu Val Pro Lys Gln Ser Val
690 695 700
Val Asp Glu Glu Asp Asp Glu Lys His Asn Thr Glu Glu Gln Ala Ile
705 710 715 720
Leu Pro Ser His Leu Ser Ser Ser Lys Lys Arg Lys Asn Phe Ser Gly
725 730 735
Ser Asp Lys Lys His Ser Lys Asn Lys Lys Gly Val Asp Asn Glu Val
740 745 750
Phe Glu Ser Val Ala Arg Pro Leu Ala Lys Val Arg Trp Phe Arg Val
755 760 765
Val Leu Asp Glu Ala Gln Ser Ile Lys Asn His Lys Thr Gln Val Ala
770 775 780
Arg Ala Cys Trp Gly Leu Arg Ala Lys Arg Arg Trp Cys Leu Ser Gly
785 790 795 800
Thr Pro Ile Gln Asn Ala Ile Asp Asp Leu Tyr Ser Tyr Phe Arg Phe
805 810 815
Leu Lys Tyr Asp Pro Tyr Ala Ala Tyr Lys Ser Phe Cys Ser Ala Ile
820 825 830
Lys Phe Pro Ile Asn Lys Asn Pro Ala Lys Gly Tyr Lys Lys Leu Gln
835 840 845
Ala Ile Leu Arg Thr Ile Met Leu Arg Arg Thr Lys Ala Thr Leu Leu
850 855 860
Asp Gly Gln Pro Ile Val Thr Leu Pro Pro Lys His Val Glu Leu Lys
865 870 875 880
Lys Val Asp Phe Thr Glu Glu Glu Arg Asp Phe Tyr Ser Lys Leu Glu
885 890 895
Ala Asp Ser Arg Ala Gln Tyr Glu Glu Tyr Ala Ala Ala Gly Thr Val
900 905 910
Lys Gln Asn Tyr Val Asn Ile Leu Leu Met Leu Leu Arg Leu Arg Gln
915 920 925
Ala Cys Asp His Pro Leu Leu Val Lys Pro Tyr Asp Ser Lys Ser Leu
930 935 940
Trp Arg Ser Ser Ala Asp Val Ala Lys Lys Leu Pro Arg Asp Lys Gln
945 950 955 960
Ile Phe Leu Leu Asn Cys Leu Glu Ala Ser Leu Ala Ile Cys Gly Ile
965 970 975
Cys Asn Asp Pro Pro Glu Asp Gly Val Val Ser Glu Cys Gly His Val
980 985 990
Phe Cys Lys Gln Cys Ile Leu Glu His Leu Ser Ser Asp Asp Cys Gln
995 1000 1005
Cys Pro Thr Gly Gly Cys Lys Val His Leu Asn Ala Ser Leu Leu Phe
1010 1015 1020
Ser Lys Ser Ser Leu Cys Asn Ser Asn Ser Asp Gln Leu Gly Glu Asp
1025 1030 1035 1040
Asn Ser Val Val Ser Ser Cys Ser Thr Val Gly Asp Ser Met Glu Leu
1045 1050 1055
Ser Ser Ser Val Met Tyr Glu Ser Ser Lys Ile Lys Ala Ala Leu Glu
1060 1065 1070
Val Leu Met Ser Leu Ala Lys Pro Lys Glu Tyr Ser Arg Asn Thr Ser
1075 1080 1085
Pro Glu Leu Ala Val Val Gly Ala Ser Glu Lys Ser Met Asp Ala Ser
1090 1095 1100
Ser Thr Glu Leu Arg Leu Glu Ser Ser Glu Cys Gln Asp Ser Thr Asn
1105 1110 1115 1120
Lys Ser Ser Cys Glu Leu Val Lys Arg Gly Gly Glu Lys Ala Ile Val
1125 1130 1135
Phe Ser Gln Trp Thr Gly Met Leu Asp Leu Leu Glu Ala Cys Leu Lys
1140 1145 1150
Asn Ser Ser Ile Gln Tyr Arg Arg Leu Asp Gly Thr Met Ser Val Leu
1155 1160 1165
Ala Arg Asp Lys Ala Val Lys Asp Phe Asn Asn Leu Pro Glu Val Ser
1170 1175 1180
Val Met Ile Met Ser Leu Lys Ala Ala Ser Leu Gly Leu Asn Met Ile
1185 1190 1195 1200
Val Ala Cys His Val Leu Leu Leu Asp Leu Trp Trp Asn Pro Thr Thr
1205 1210 1215
Glu Asp Gln Ala Ile Asp Arg Ala His Arg Ile Gly Gln Thr Arg Pro
1220 1225 1230
Val Thr Val Leu Arg Leu Thr Val Arg Asp Thr Val Glu Asp Arg Ile
1235 1240 1245
Leu Ala Leu Gln Gln Lys Lys Arg Glu Met Val Ser Ser Ala Phe Gly
1250 1255 1260
Glu Asp Glu Ala Gly Gly Arg Gln Thr Arg Leu Thr Val Glu Asp Leu
1265 1270 1275 1280
Asn Tyr Leu Phe Met Met
1285
<210> 137
<211> 1046
<212> PRT
<213> 琴叶拟南芥
<400> 137
Met Asp Ser Ala Ile Glu Ile Ser Ser Gly Ser Asp Ser Asp Asp Glu
1 5 10 15
Leu Pro Gln Pro Ile Arg Pro Gln Ile Arg Thr Arg Thr Asp Pro Ile
20 25 30
Trp Leu Gly Ile Glu Ile Val Pro Thr Val Asp Ser His Ala Arg Ala
35 40 45
Glu His Thr Asn Gln Ala Ser Arg Asn Ser Ala Ser Asn Asp Thr Ser
50 55 60
Arg Pro Gly Val Ser Lys Pro Phe Thr Gly Asn Gly Asn Thr Val Asn
65 70 75 80
Ser Arg Ile Ser Ser Gly Ser Gly Ala Asp Tyr Glu Arg Leu Ser Ser
85 90 95
Glu Gln Ala Pro Lys Arg Thr Leu Pro Pro Ser Phe Ser Ser Pro Pro
100 105 110
Phe Pro Ser Arg Ser Gly Ala Asn Asn Ile Ser Asn Ala Ser Gly Ser
115 120 125
Arg Val Gly Ala Asp His Glu Arg Leu Ser Ser Gln Gln Ala Pro Lys
130 135 140
Arg Thr Leu Pro Pro Ser Phe Asn Pro Pro Pro Leu Pro Ser Arg Ser
145 150 155 160
Gly Thr Asn Asn Ile Ser Asn Ala Ser Gly Ser Arg Phe Gly Ala Asp
165 170 175
Tyr Ser Arg Pro Ala Val Ser Ala Val Gly Asn Lys Ser Thr Phe Gly
180 185 190
Asp His Tyr Ser Gly Ala His Ala Glu Ile Gly Ile Gln Arg Gly Met
195 200 205
Asn Gly Val Arg Ile Leu Pro Pro Ser Leu Thr His Gly Thr Ser Ala
210 215 220
Ser Val Leu His His Ala Gly Ser Ser Asp Pro Met His Arg Leu Gly
225 230 235 240
Thr Gly Glu Asp Arg Asn Pro Asp Asn Asp Glu Arg Leu Val Tyr Gln
245 250 255
Ala Ala Leu Gln Asp Leu Asn Gln Pro Ile Thr Glu Ser Asp Leu Pro
260 265 270
Pro Gly Val Leu Ser Val Pro Leu Met Arg His Gln Lys Ile Ala Leu
275 280 285
Ala Trp Met Phe Gln Lys Glu Thr Arg Ser Phe Asn Cys Ala Gly Gly
290 295 300
Ile Leu Ala Asp Asp Gln Gly Leu Gly Lys Thr Val Ser Thr Ile Ala
305 310 315 320
Leu Ile Leu Lys Gln Lys Ile Val Ser Gln Leu Lys Ser Ala Asn Ser
325 330 335
Cys Lys Gln Glu Thr Glu Ala Leu Val Leu Asp Ala Asp Asp Glu Ser
340 345 350
Asp Asn Ala Lys His Glu Asn Gly Ser His Val Lys Pro Glu Leu Lys
355 360 365
Val Ser Ser Asn Ser Glu Thr Ser Val Leu Ser Ala Ser Gly Asn Asp
370 375 380
Glu Asn Asp Ser Ser Asp Met Glu Lys Ala Lys Asp Glu Glu Ala Asn
385 390 395 400
Ser Ser Thr Arg Ala Phe Lys Trp Lys Arg Pro Ala Ala Gly Thr Leu
405 410 415
Ile Val Cys Pro Ala Ser Val Val Arg Gln Trp Ala Arg Glu Leu Asp
420 425 430
Glu Lys Val Ser Glu Glu Trp Lys Leu Ser Val Leu Val Tyr His Gly
435 440 445
Ser Asn Arg Thr Lys Asp Pro Asn Glu Leu Ala Glu Tyr Asp Val Val
450 455 460
Val Thr Thr Tyr Ala Ile Val Thr Asn Glu Ala Pro Lys Lys Phe Leu
465 470 475 480
Val Asp Glu Asp Glu Asn Asp Glu Lys Ser Thr Asp Glu Tyr Gly Leu
485 490 495
Ala Ser Gly Phe Ser Asn Asn Lys Lys Arg Lys Val Ala Val Gly Ala
500 505 510
Ser Lys Lys Ser Lys Lys Arg Ser Arg Lys Ser Thr Asp Asn Ser Ser
515 520 525
Ser Glu Pro Asp Cys Gly Ala Leu Gly Lys Val Gly Trp Phe Arg Ile
530 535 540
Val Leu Asp Glu Ala Gln Thr Ile Lys Asn His Arg Thr Gln Val Ala
545 550 555 560
Arg Ser Cys Ser Thr Leu Arg Ala Lys Arg Arg Trp Cys Leu Ser Gly
565 570 575
Thr Pro Ile Gln Asn Thr Ile Asp Asp Leu Tyr Ser Tyr Phe Arg Phe
580 585 590
Leu Arg Tyr Asp Pro Tyr Ala Ala Tyr Lys Ser Phe Tyr Ser Thr Ile
595 600 605
Lys Val Pro Ile Ser Arg Asn Ser Cys Gln Gly Tyr Lys Lys Leu Gln
610 615 620
Ala Val Leu Arg Ala Ile Met Leu Arg Arg Thr Lys Gly Thr Leu Leu
625 630 635 640
Asp Gly Lys Pro Ile Ile Asn Leu Pro Pro Lys Lys Val Asn Leu Ser
645 650 655
Thr Val Asp Phe Ser Val Glu Glu Arg Ser Phe Tyr Arg Lys Leu Glu
660 665 670
Ala Asp Ser Arg Ser Gln Phe Lys Ala Tyr Ala Asp Ala Gly Thr Leu
675 680 685
Ser Gln Asn Tyr Ala Asn Ile Leu Leu Met Leu Leu Arg Leu Arg Gln
690 695 700
Ala Cys Asp His Pro Gln Leu Val Lys Val Tyr Asn Ser Asp Pro Val
705 710 715 720
Gly Lys Glu Ser Glu Ala Ala Val Arg Arg Leu Pro Arg Glu Ala Arg
725 730 735
Ser Arg Leu Ile Asn Arg Leu Glu Ser Ser Ser Ala Ile Cys Tyr Glu
740 745 750
Cys Asn Glu Pro Pro Glu Lys Pro Val Val Thr Leu Cys Gly His Val
755 760 765
Phe Cys Tyr Glu Cys Val Leu Glu Tyr Ile Thr Gly Asp Glu Asn Met
770 775 780
Cys Pro Val Pro Arg Cys Lys Gln Gln Leu Ala Arg Asp Val Val Phe
785 790 795 800
Ser Glu Ser Ser Leu Arg Asn Cys Ile Ser Asp Asp Leu Gly Cys Ser
805 810 815
Ser Ser His Asp Lys Gly Leu Asp Arg Ser Val Phe Glu Lys Arg Glu
820 825 830
Phe Cys Ser Ser Lys Ile Lys Ala Val Leu Asp Ile Leu Gln Ser Leu
835 840 845
Ser Lys Gln Asp Thr Pro Asn Ser Ala Gln His Gly Gln Met Pro Ser
850 855 860
Ser Ser Gly Pro Tyr Asp Asp Asp Asp Val Thr Ile Val Glu Pro Met
865 870 875 880
Arg Leu His Ser Ser Ser Pro Ser Gln Gly Ala Val Lys Thr Ile Ile
885 890 895
Phe Ser Gln Trp Thr Gly Met Leu Asp Leu Val Glu Leu Arg Ile Leu
900 905 910
Glu Ser Gly Ile Glu Phe Arg Arg Leu Asp Gly Thr Met Ser Leu Ala
915 920 925
Ala Arg Asp Arg Ala Val Lys Glu Phe Ser Lys Asn Pro Asp Val Lys
930 935 940
Val Met Leu Met Ser Leu Lys Ala Gly Asn Leu Gly Leu Asn Met Val
945 950 955 960
Ala Ala Cys His Val Ile Leu Leu Asp Leu Trp Trp Asn Pro Thr Thr
965 970 975
Glu Asp Gln Ala Ile Asp Arg Ala His Arg Ile Gly Gln Thr Arg Pro
980 985 990
Val Thr Val Thr Arg Ile Thr Ile Lys Asp Thr Val Glu Asp Arg Ile
995 1000 1005
Leu Thr Leu Gln Glu Asp Lys Arg Thr Met Val Ala Ser Ala Phe Gly
1010 1015 1020
Glu Glu His Gly Gly Ser Ser Ala Thr Arg Leu Thr Val Asp Asp Leu
1025 1030 1035 1040
Lys Tyr Leu Phe Met Leu
1045
<210> 138
<211> 630
<212> PRT
<213> 人工序列
<220>
<223> 合成的构建体
<400> 138
Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys
1 5 10 15
Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala
20 25 30
His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn His Gly
35 40 45
Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys
50 55 60
Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn
65 70 75 80
His Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val
85 90 95
Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala
100 105 110
Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu
115 120 125
Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala
130 135 140
Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg
145 150 155 160
Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val
165 170 175
Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val
180 185 190
Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu
195 200 205
Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu
210 215 220
Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr
225 230 235 240
Pro Glu Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala
245 250 255
Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly
260 265 270
Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys
275 280 285
Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala
290 295 300
His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly
305 310 315 320
Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys
325 330 335
Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn
340 345 350
Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val
355 360 365
Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala
370 375 380
Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu
385 390 395 400
Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala
405 410 415
Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg
420 425 430
Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val
435 440 445
Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val
450 455 460
Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu
465 470 475 480
Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu
485 490 495
Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr
500 505 510
Pro Glu Gln Val Val Ala Ile Ala Ser Asn Gly Gly Lys Gln Ala Leu
515 520 525
Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu
530 535 540
Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln
545 550 555 560
Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His
565 570 575
Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly
580 585 590
Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln
595 600 605
Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Gly
610 615 620
Gly Arg Pro Ala Leu Glu
625 630
<210> 139
<211> 631
<212> PRT
<213> 人工序列
<220>
<223> 合成的构建体
<400> 139
Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys
1 5 10 15
Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala
20 25 30
His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Gly Gly
35 40 45
Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys
50 55 60
Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn
65 70 75 80
Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val
85 90 95
Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala
100 105 110
Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu
115 120 125
Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala
130 135 140
Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg
145 150 155 160
Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val
165 170 175
Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val
180 185 190
Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu
195 200 205
Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu
210 215 220
Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr
225 230 235 240
Pro Glu Gln Val Val Ala Ile Ala Ser Asn Gly Gly Lys Gln Ala Leu
245 250 255
Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu
260 265 270
Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln
275 280 285
Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His
290 295 300
Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn His Gly Gly
305 310 315 320
Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln
325 330 335
Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Gly
340 345 350
Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu
355 360 365
Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser
370 375 380
Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro
385 390 395 400
Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile
405 410 415
Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu
420 425 430
Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val
435 440 445
Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln
450 455 460
Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln
465 470 475 480
Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr
485 490 495
Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro
500 505 510
Glu Gln Val Val Ala Ile Ala Ser Asn His Gly Gly Lys Gln Ala Leu
515 520 525
Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu
530 535 540
Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln
545 550 555 560
Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His
565 570 575
Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly
580 585 590
Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln
595 600 605
Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile
610 615 620
Gly Gly Arg Pro Ala Leu Glu
625 630
<210> 140
<211> 629
<212> PRT
<213> 人工序列
<220>
<223> 合成的构建体
<400> 140
Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys
1 5 10 15
Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala
20 25 30
His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Gly Gly
35 40 45
Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys
50 55 60
Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn
65 70 75 80
Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val
85 90 95
Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala
100 105 110
Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu
115 120 125
Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala
130 135 140
Ile Ala Ser Asn His Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg
145 150 155 160
Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val
165 170 175
Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val
180 185 190
Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu
195 200 205
Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu
210 215 220
Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr
225 230 235 240
Pro Glu Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala
245 250 255
Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly
260 265 270
Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys
275 280 285
Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala
290 295 300
His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Asn Gly
305 310 315 320
Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys
325 330 335
Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn
340 345 350
Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu
355 360 365
Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser
370 375 380
Asn His Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro
385 390 395 400
Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile
405 410 415
Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu
420 425 430
Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val
435 440 445
Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln
450 455 460
Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln
465 470 475 480
Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr
485 490 495
Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro
500 505 510
Glu Gln Val Val Ala Ile Ala Ser Asn Gly Gly Lys Gln Ala Leu Glu
515 520 525
Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr
530 535 540
Pro Glu Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala
545 550 555 560
Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly
565 570 575
Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys
580 585 590
Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala
595 600 605
His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Gly Gly
610 615 620
Arg Pro Ala Leu Glu
625
<210> 141
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 141
cggaaagatg tatgggct 18
<210> 142
<211> 35
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 142
gggagagtga acgttggtga cctgcttcta tgttt 35
<210> 143
<211> 35
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 143
aaacatagaa gcaggtcacc aacgttcact ctccc 35
<210> 144
<211> 52
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 144
ccaaggacct ctcgagggat cttcaggttc atcttcgcgg acccggctcc ct 52
<210> 145
<211> 49
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 145
catagatccc tcgagtgaag aaccagatga tccgctagct gacgcgcga 49
<210> 146
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 146
ttaggatttg cgtgtcgac 19
<210> 147
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 147
gtggcaattg cgagcaacgg gggaaagcag 30
<210> 148
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 148
ctgctttccc ccgttgctcg caattgccac 30
<210> 149
<211> 35
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 149
ggcaattgcg agcaaccatg ggggaaagca ggcac 35
<210> 150
<211> 35
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 150
gtgcctgctt tcccccatgg ttgctcgcaa ttgcc 35
<210> 151
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 151
tggctattgc atccaacggg ggcagacc 28
<210> 152
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 152
ggtctgcccc cgttggatgc aatagcca 28
<210> 153
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 153
agcacggatc gaatcacata 20
<210> 154
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 154
ctcgctgctt ctcgaatctt 20
<210> 155
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 155
cgggtccttg gactcctgat 20
<210> 156
<211> 23
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 156
tcgtgaccgg aataattaaa tgg 23
<210> 157
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 157
ggcttcgata ggaagaatgc cc 22
<210> 158
<211> 23
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 158
gtgaaactgc cagatccaaa ttc 23
<210> 159
<211> 29
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 159
tcccgagaag agtagaacaa atgctaaaa 29
<210> 160
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 160
ctgaggtatt ccatagcccc tgatcc 26
<210> 161
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 161
tggaatctca gggtcaagg 19
<210> 162
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 162
ccttctgagg tgagggaca 19
<210> 163
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 163
aagagttatg ggccgaagc 19
<210> 164
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 164
cgctcgtatg aatgttgaat g 21
<210> 165
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 165
ataaagagcg gcgcaagat 19
<210> 166
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 166
cgctctaggg tttttgcttt 20
<210> 167
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 167
aggccccatc tcacaaatac 20
<210> 168
<211> 35
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 168
tcgttgtctc gccgaaattc gaaagaccga gagag 35
<210> 169
<211> 47
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 169
cctttaattt ccttttttgt ttgcgtctct actctctaca atctata 47
<210> 170
<211> 35
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 170
gggagagtga acgttggtga cctgcttcta tgttt 35
<210> 171
<211> 35
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 171
aaacatagaa gcaggtcacc aacgttcact ctccc 35
<210> 172
<211> 50
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 172
ggtatcccat ctcgagtgaa gaaccagatg atccgctagc tgacgcgcga 50
<210> 173
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 173
ggggatttga taatgcgtc 19
<210> 174
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 174
gttaagactg tgaaagaga 19
<210> 175
<211> 43
<212> DNA
<213> 人工序列
<220>
<223> 合成的构建体
<400> 175
tcatcatagt gttgttatat ctttggtgtc tctgtcttgc ttc 43

Claims (17)

1.一种用于减少植物中一种或更多种靶核酸的表达的方法，所述方法包括：
(a)提供包含重组核酸的植物，其中所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的重组多肽，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含DMS3多肽或其片段；以及
(b)在由所述重组核酸编码的所述重组多肽借以被表达并与所述一种或更多种靶核酸结合的条件下使所述植物生长，从而减少所述一种或更多种靶核酸的表达。
2.如权利要求1所述的方法，其中所述DNA结合结构域包含锌指结构域。
3.如权利要求1所述的方法，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
4.如权利要求1所述的方法，其中所述DNA结合结构域包含TAL效应子靶向结构域。
5.如权利要求1所述的方法，其中所述第二氨基酸序列包含与SEQ ID NO:41至少80％相同的氨基酸序列。
6.如权利要求5所述的方法，其中所述第二氨基酸序列包含与SEQ ID NO:41 100％相同的氨基酸序列。
7.如权利要求1所述的方法，其中所述一种或更多种靶核酸为内源核酸。
8.如权利要求1所述的方法，其中所述一种或更多种靶核酸为异源核酸。
9.如权利要求1所述的方法，其中所述一种或更多种靶核酸的表达被沉默。
10.一种重组核酸，所述重组核酸编码包含第一氨基酸序列和第二氨基酸序列的DMS3样蛋白，所述第一氨基酸序列包含DNA结合结构域，所述第二氨基酸序列包含DMS3多肽或其片段。
11.如权利要求10所述的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:41至少80％相同的氨基酸序列。
12.如权利要求11所述的重组核酸，其中所述第二氨基酸序列包含与SEQ ID NO:41100％相同的氨基酸序列。
13.如权利要求10所述的重组核酸，其中所述DNA结合结构域包含锌指结构域。
14.如权利要求10所述的重组核酸，其中所述DNA结合结构域选自由以下组成的组：TAL效应子靶向结构域、螺旋-转角-螺旋家族DNA结合结构域、碱性结构域、带-螺旋-螺旋结构域、TBP结构域、桶状二聚体结构域、真实同源结构域、BAH结构域、SANT结构域、克罗莫结构域、Tudor结构域、布罗莫结构域、PHD结构域、WD40结构域和MBD结构域。
15.如权利要求10所述的重组核酸，其中所述DNA结合结构域包含TAL效应子靶向结构域。
16.一种宿主细胞，所述宿主细胞包含表达载体，其中所述表达载体包含权利要求10到15中任一项所述的重组核酸，其中所述重组核酸被可操作地连接至调节序列。
17.如权利要求16所述的宿主细胞，其中所述宿主细胞为植物细胞。
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