BR112019007327A2 - método para obter uma célula vegetal, célula vegetal, planta, semente, polinucleotídeo-guia - Google Patents

método para obter uma célula vegetal, célula vegetal, planta, semente, polinucleotídeo-guia

Info

Publication number
BR112019007327A2
BR112019007327A2 BR112019007327A BR112019007327A BR112019007327A2 BR 112019007327 A2 BR112019007327 A2 BR 112019007327A2 BR 112019007327 A BR112019007327 A BR 112019007327A BR 112019007327 A BR112019007327 A BR 112019007327A BR 112019007327 A2 BR112019007327 A2 BR 112019007327A2
Authority
BR
Brazil
Prior art keywords
plant
plant cell
nlb18
methods
obtaining
Prior art date
Application number
BR112019007327A
Other languages
English (en)
Inventor
Li Bailin
M Tabor Girma
Gao Huirong
Daniel Perugini Leandro
B Meeley Robert
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of BR112019007327A2 publication Critical patent/BR112019007327A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

trata-se de composições e métodos para obter células vegetais com sequências de nucleotídeos de ht1 modificadas, sequências de nlb18 modificadas ou ambas. os métodos envolvem introduzir quebras de fita dupla no genoma de maís em uma sequência de codificação de ht1 endógena, uma sequência de codificação de nlb18 endógena ou ambas, para modificar a sequência genômica de modo a aprimorar resistência à helmintosporiose de uma planta produzida da célula vegetal. são fornecidos, adicionalmente, métodos que introduzem alelos resistentes de ht1 e/ou nlb18 em sítios específicos no genoma. plantas produzidas pelas células vegetais, e sementes produzidas das plantas também são incluídas. polinucleotídeos-guia também são fornecidos para o uso do sistema de crispr-cas na indução de quebras de fita dupla.
BR112019007327A 2016-10-13 2017-10-10 método para obter uma célula vegetal, célula vegetal, planta, semente, polinucleotídeo-guia BR112019007327A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662407867P 2016-10-13 2016-10-13
PCT/US2017/055835 WO2018071362A1 (en) 2016-10-13 2017-10-10 Generating northern leaf blight resistant maize

Publications (1)

Publication Number Publication Date
BR112019007327A2 true BR112019007327A2 (pt) 2019-07-02

Family

ID=60153565

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112019007327A BR112019007327A2 (pt) 2016-10-13 2017-10-10 método para obter uma célula vegetal, célula vegetal, planta, semente, polinucleotídeo-guia

Country Status (10)

Country Link
US (1) US12012605B2 (pt)
EP (2) EP3525578A1 (pt)
CN (1) CN109844121A (pt)
AR (1) AR109941A1 (pt)
BR (1) BR112019007327A2 (pt)
CA (1) CA3037560A1 (pt)
EA (1) EA201990924A1 (pt)
UA (1) UA128281C2 (pt)
WO (1) WO2018071362A1 (pt)
ZA (1) ZA201901854B (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011163590A1 (en) 2010-06-25 2011-12-29 E. I. Du Pont De Nemours And Company Compositions and methods for enhancing resistance to northern leaf blight in maize
EP3362469B1 (en) 2015-10-16 2021-03-24 Pioneer Hi-Bred International, Inc. Generating maize plants with enhanced resistance to northern leaf blight
WO2021257206A1 (en) * 2020-06-17 2021-12-23 Pioneer Hi-Bred International, Inc. Generating maize plants with enhanced resistance to northern leaf blight
UA128281C2 (uk) 2016-10-13 2024-05-29 Піонір Хай-Бред Інтернешнл, Інк. Одержання маїсу, стійкого до північної плямистості листя
EP3447134B1 (en) * 2017-08-22 2023-10-11 KWS SAAT SE & Co. KGaA Increased fungal resistance in crop plants
WO2020023258A1 (en) * 2018-07-23 2020-01-30 Pioneer Hi-Bred International, Inc. Methods and compositions to increase yield through modifications of fea3 genomic locus and associated ligands
WO2021141890A1 (en) * 2020-01-09 2021-07-15 Pioneer Hi-Bred International, Inc. Two-step gene swap
EP4200425A1 (en) * 2020-08-18 2023-06-28 Pioneer Hi-Bred International, Inc. Multiple disease resistance genes and genomic stacks thereof
WO2023164453A2 (en) * 2022-02-23 2023-08-31 Pioneer Hi-Bred International, Inc. Multiple disease resistance genes and genomic stacks thereof

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5844116A (en) 1997-01-29 1998-12-01 Pioneer Hi-Bred International, Inc. Inbred maize line PH1W2
US6504084B1 (en) 1999-04-23 2003-01-07 Pioneer Hi-Bred International, Inc. Maize NPR1 polynucleotides and methods of use
US6765132B1 (en) 2000-05-02 2004-07-20 Pioneer Hi-Bred International, Inc. Inbred maize line PH26N
US6720487B1 (en) 2001-01-11 2004-04-13 Pioneer Hi-Bred International, Inc. Inbred maize line PH4GP
ATE421697T1 (de) 2002-04-12 2009-02-15 Dowd Brian F O Verfahren zur identifizierung von verbindungen, die transmembran-proteine beeinflussen
EP1874935B2 (en) 2005-04-04 2023-10-18 E. I. du Pont de Nemours and Company Polynucleotides and methods for making plants resistant to fungal pathogens
CA2961849C (en) 2005-08-26 2022-08-02 Dupont Nutrition Biosciences Aps Use of crispr associated genes (cas)
US20080083042A1 (en) 2006-08-14 2008-04-03 David Butruille Maize polymorphisms and methods of genotyping
US20120017338A1 (en) 2008-01-15 2012-01-19 Wei Wu Isolated novel nucleic acid and protein molecules from corn and methods of using those molecules to generate transgenic plant with enhanced agronomic traits
MX2011003866A (es) 2008-10-13 2011-05-02 Du Pont Loci geneticos relacionados con la resistencia al tizon del norte de la hoja del maiz.
US9539510B2 (en) 2010-04-30 2017-01-10 Microsoft Technology Licensing, Llc Reshapable connector with variable rigidity
US8445763B1 (en) 2010-05-25 2013-05-21 Pioneer Hi-Bred International, Inc. Inbred maize variety PH184C
WO2011163590A1 (en) 2010-06-25 2011-12-29 E. I. Du Pont De Nemours And Company Compositions and methods for enhancing resistance to northern leaf blight in maize
US20220064662A1 (en) 2010-06-25 2022-03-03 E. I. Du Pont De Nemours And Company Compositions and methods for enhancing resistance to northern leaf blight in maize
US10030245B2 (en) 2011-03-23 2018-07-24 E I Du Pont De Nemours And Company Methods for producing a complex transgenic trait locus
DK2800811T3 (en) 2012-05-25 2017-07-17 Univ Vienna METHODS AND COMPOSITIONS FOR RNA DIRECTIVE TARGET DNA MODIFICATION AND FOR RNA DIRECTIVE MODULATION OF TRANSCRIPTION
WO2014036048A1 (en) 2012-08-30 2014-03-06 E. I. Du Pont De Nemours And Company Long intergenic non-coding rnas in maize
EP3825401A1 (en) 2012-12-12 2021-05-26 The Broad Institute, Inc. Crispr-cas component systems, methods and compositions for sequence manipulation
BR112016003591A8 (pt) 2013-08-22 2018-01-30 Du Pont promotor u6 de polimerase iii de soja e métodos de uso
US10897862B2 (en) 2013-09-04 2021-01-26 KWS SAAT SE & Co. KGaA Plant resistant to Helminthosporium turcicum
US20150315605A1 (en) * 2014-02-21 2015-11-05 E I Du Pont De Nemours And Company Novel transcripts and uses thereof for improvement of agronomic characteristics in crop plants
WO2016007347A1 (en) 2014-07-11 2016-01-14 E. I. Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
US10513711B2 (en) 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
EP3191595B1 (en) 2014-09-12 2019-12-25 E. I. du Pont de Nemours and Company Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use
EP3597740B1 (en) 2014-11-06 2022-03-23 DuPont US Holding, LLC Peptide-mediated delivery of rna-guided endonuclease into cells
US20220408678A1 (en) 2015-10-16 2022-12-29 Pioneer Hi-Bred International, Inc. Generating maize plants with enhanced resistance to northern leaf blight
WO2021257206A1 (en) 2020-06-17 2021-12-23 Pioneer Hi-Bred International, Inc. Generating maize plants with enhanced resistance to northern leaf blight
US20230210080A1 (en) 2015-10-16 2023-07-06 Pioneer Hi-Bred International, Inc. Generating maize plants with enhanced resistance to northern leaf blight
EP3362469B1 (en) * 2015-10-16 2021-03-24 Pioneer Hi-Bred International, Inc. Generating maize plants with enhanced resistance to northern leaf blight
US20210274739A1 (en) 2015-10-16 2021-09-09 Pioneer Hi-Bred International, Inc. Generating maize plants with enhanced resistance to northern leaf blight
UA128281C2 (uk) 2016-10-13 2024-05-29 Піонір Хай-Бред Інтернешнл, Інк. Одержання маїсу, стійкого до північної плямистості листя

Also Published As

Publication number Publication date
ZA201901854B (en) 2019-11-27
EP4242330A3 (en) 2023-11-22
EA201990924A1 (ru) 2019-09-30
EP4242330A2 (en) 2023-09-13
CA3037560A1 (en) 2018-04-19
EP3525578A1 (en) 2019-08-21
US20220275392A1 (en) 2022-09-01
AR109941A1 (es) 2019-02-06
UA128281C2 (uk) 2024-05-29
US12012605B2 (en) 2024-06-18
WO2018071362A1 (en) 2018-04-19
CN109844121A (zh) 2019-06-04

Similar Documents

Publication Publication Date Title
BR112019007327A2 (pt) método para obter uma célula vegetal, célula vegetal, planta, semente, polinucleotídeo-guia
BR112016007566A2 (pt) evento de milho transgênico mon87403 e métodos para a detecção do mesmo
BR112018076027A2 (pt) método para modificar uma sequência-alvo no genoma de uma célula vegetal; método para editar uma sequência de nucleotídeos no genoma de uma célula vegetal; método para modificar simultaneamente múltiplas sequências-alvo no genoma de uma célula vegetal; método para modificar uma sequênciaalvo de dna no genoma de uma célula vegetal e modelo de modificação de polinucleotídeo
BR112017018327A2 (pt) método para a expressão regulada de um complexo, planta ou célula vegetal, semente, método para modificação de uma sequência, método para alteração da expressão de pelo menos um polinucleotídeo
MX2018004809A (es) Metodos y composiciones para la modificacion genomica exenta de marcadores.
BR112017000621A2 (pt) método para melhorar um traço agronômico de uma planta, método para melhorar a produtividade de grãos ou a eficiência de uso de nitrogênio de uma planta de milho ou de cultura, método para editar, substituir ou eliminar uma sequência reguladora, método para inserir um promotor ou elemento do promotor no genoma de uma célula vegetal e método para fornecer um perfil de expressão adicional para um polinucleotídeo endógeno de uma célula vegetal
BR112013024337A2 (pt) locus de traço transgênico complexo em uma planta, planta ou semente, método para produzir em uma planta um locus de traço transgênico complexo e construto de expressão
AR100216A1 (es) Edición de genes multiplexados
BR112016019267A2 (pt) métodos e composições para perfil de dna
BR112016003776A2 (pt) polinucleotídeo, planta ou semente, complexo de polinucleotídeo, método para modificação de um sítio-alvo no genoma de uma célula, método para introduzir um polinucleotídeo de interesse, método para editar uma sequência de nucleotídeos, célula vegetal, método para selecionar uma planta
BR112021018159A2 (pt) Método para gerar uma molécula de rna com atividade de silenciamento, célula geneticamente modificada, planta geneticamente modificada, célula vegetal, planta, semente, métodos de produção de uma planta, de tratamento de uma doença e de introdução da atividade de silenciamento
BR112013009001A2 (pt) planta e semente de soja correspondendo a evento transgênico mon87712 e métodos para deteção das mesmas
BR112012009144B8 (pt) rostafuroxina, método para a identificação de um indivíduo com melhor resposta à rostafuroxina e kit de partes para avaliar o tratamento de rostafuroxina para um indivíduo
AR081683A1 (es) Composiciones y metodos para mejorar la resistencia al tizon norteño de las hojas en maiz
BR112017017279A2 (pt) métodos para introduzir um gene inibidor de pólen, para introduzir dois genes inibidores de pólen, para introduzir dois genes marcadores coloridos e de introgressão acelerada de traço e planta
PE20091023A1 (es) Plantas y semillas de soja correspondientes al evento transgenico mon87701 y metodos de deteccion del mismo
BR112017023304A2 (pt) sequência de ácido nucleico, método para detectar a presença do dna, kit de detecção de dna, célula ou parte da planta, método para a proteção de uma planta de milho, método para a reprodução de uma planta, método para a produção de uma planta de milho, composição, produto ou artigo agrícola
BRPI0915963A2 (pt) mercadores associados com resistência à ferrugem de soja e métodos de uso destes
BR112016023624A2 (pt) proteínas de antiporte prótons/ açúcar tonoplásticas e uso do mesmo para o aumento da concentração de sacarose de um órgão de plantas para armazenamento de sacarose
BR112017012900A2 (pt) polinucleotídeo isolado ou um dna recombinante, célula de planta, planta, explante, semente, método para produzir uma célula de planta, método para controlar ervas daninhas, célula hospedeira
BR112018072154A2 (pt) construções genéticas recombinantes, métodos para produzir uma construção genética recombinante e para melhorar geneticamente uma planta, vetor, célula hospedeira e plantas
BR112017026015A2 (pt) método para identificar e/ou selecionar uma planta de milho com resistência à podridão de colmo causada por antracnose, e método para introgredir um alelo qtl associado à resistência à podridão de colmo causada por antracnose em uma planta de milho
BR112013028406B8 (pt) Método para identificar alvos de antibióticos
BR112017002448A2 (pt) método de identificação de planta de milho com helmintosporiose e método de introgressão de alelos
BR112016016772A2 (pt) Método para obter células vegetais transformadas

Legal Events

Date Code Title Description
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]