BR112016016772A2 - Método para obter células vegetais transformadas - Google Patents

Método para obter células vegetais transformadas

Info

Publication number
BR112016016772A2
BR112016016772A2 BR112016016772A BR112016016772A BR112016016772A2 BR 112016016772 A2 BR112016016772 A2 BR 112016016772A2 BR 112016016772 A BR112016016772 A BR 112016016772A BR 112016016772 A BR112016016772 A BR 112016016772A BR 112016016772 A2 BR112016016772 A2 BR 112016016772A2
Authority
BR
Brazil
Prior art keywords
transformed plant
plant cells
transformed
target dna
marker gene
Prior art date
Application number
BR112016016772A
Other languages
English (en)
Inventor
Hiei Yukoh
Komari Toshihiko
Original Assignee
Japan Tobacco Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Tobacco Inc filed Critical Japan Tobacco Inc
Publication of BR112016016772A2 publication Critical patent/BR112016016772A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C12N2830/003—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor tet inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
    • C12N2830/006—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB tet repressible

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

MÉTODO PARA OBTER CÉLULAS VEGETAIS TRANSFORMADAS. A presente invenção refere-se a um método para obter uma célula de planta transformada. A presente invenção compreende as etapas de: (a) cotransformar um DNA destinado e um primeiro gene marcador em uma célula vegetal; e (b) selecionar a partir das células transformadas obtidas na etapa (a), uma célula de planta transformada em que o DNA destinado é introduzido em um cromossomo da mesma, e o primeiro gene marcador não é introduzido, em que o método não contém uma etapa para excluir uma célula transformada apenas com o DNA destinado introduzido no cromossomo por seleção positiva usando o primeiro gene marcador.
BR112016016772A 2014-02-06 2015-02-04 Método para obter células vegetais transformadas BR112016016772A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/JP2014/052765 WO2015118640A1 (ja) 2014-02-06 2014-02-06 植物の形質転換細胞の取得方法
PCT/JP2015/053137 WO2015119166A1 (ja) 2014-02-06 2015-02-04 植物の形質転換細胞の取得方法

Publications (1)

Publication Number Publication Date
BR112016016772A2 true BR112016016772A2 (pt) 2017-10-03

Family

ID=53777473

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016016772A BR112016016772A2 (pt) 2014-02-06 2015-02-04 Método para obter células vegetais transformadas

Country Status (7)

Country Link
US (1) US11136587B2 (pt)
EP (1) EP3103870B1 (pt)
JP (1) JPWO2015118640A1 (pt)
CN (1) CN105960458B (pt)
AU (1) AU2015215536B2 (pt)
BR (1) BR112016016772A2 (pt)
WO (2) WO2015118640A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11371056B2 (en) 2017-03-07 2022-06-28 BASF Agricultural Solutions Seed US LLC HPPD variants and methods of use
CA3055389A1 (en) 2017-03-07 2018-09-13 BASF Agricultural Solutions Seed US LLC Hppd variants and methods of use

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002514927A (ja) * 1997-06-30 2002-05-21 シンジェンタ モーヘン ビー．ブイ． 植物の形質転換のためのプラスミドならびにその使用方法
EP2272965A3 (en) * 2001-07-06 2011-01-19 Monsanto Technology LLC Methods for enhancing segregation of transgenes in plants and compositions thereof
US7575917B2 (en) * 2003-04-09 2009-08-18 Monsanto Technology Llc DNA constructs and methods to enhance the production of commercially viable transgenic plants
WO2008001414A1 (fr) 2006-06-23 2008-01-03 Japan Tobacco Inc. Vecteur cosmide destiné à transformer une plante et son procédé d'utilisation
EP3023499A1 (en) 2008-07-16 2016-05-25 Monsanto Technology LLC Methods and vectors for producing transgenic plants

Also Published As

Publication number Publication date
WO2015118640A1 (ja) 2015-08-13
US11136587B2 (en) 2021-10-05
EP3103870B1 (en) 2020-10-21
AU2015215536B2 (en) 2021-02-18
AU2015215536A1 (en) 2016-08-25
CN105960458A (zh) 2016-09-21
US20160340682A1 (en) 2016-11-24
WO2015119166A1 (ja) 2015-08-13
EP3103870A4 (en) 2017-09-20
JPWO2015118640A1 (ja) 2017-03-23
CN105960458B (zh) 2019-09-10
EP3103870A1 (en) 2016-12-14

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