BR112019002505A2 - método para produzir um agregado de proteína recombinante, e, agregado de proteína recombinante. - Google Patents
método para produzir um agregado de proteína recombinante, e, agregado de proteína recombinante.Info
- Publication number
- BR112019002505A2 BR112019002505A2 BR112019002505A BR112019002505A BR112019002505A2 BR 112019002505 A2 BR112019002505 A2 BR 112019002505A2 BR 112019002505 A BR112019002505 A BR 112019002505A BR 112019002505 A BR112019002505 A BR 112019002505A BR 112019002505 A2 BR112019002505 A2 BR 112019002505A2
- Authority
- BR
- Brazil
- Prior art keywords
- recombinant protein
- protein aggregate
- recombinant
- producing
- insoluble bodies
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
- C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Water Supply & Treatment (AREA)
- Insects & Arthropods (AREA)
- Tropical Medicine & Parasitology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
o propósito da presente invenção é prover um método para separar de forma eficiente corpos insolúveis de uma proteína recombinante alvo de uma célula recombinante que expressa a proteína recombinante dentro da mesma como corpos insolúveis. a presente invenção provê um método de produção no qual corpos insolúveis de uma proteína recombinante são separados de células recombinantes que expressam a proteína recombinante dentro das mesmas como corpos insolúveis. as células recombinantes são fragmentadas, e então os corpos insolúveis da proteína recombinante são agregados e separados.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2016157912 | 2016-08-10 | ||
| JP2016229227 | 2016-11-25 | ||
| JP2017048702 | 2017-03-14 | ||
| JP2017094144 | 2017-05-10 | ||
| PCT/JP2017/029033 WO2018030499A1 (ja) | 2016-08-10 | 2017-08-10 | 不溶性組換えタンパク質凝集体の製造方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112019002505A2 true BR112019002505A2 (pt) | 2019-06-25 |
Family
ID=61162268
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112019002505A BR112019002505A2 (pt) | 2016-08-10 | 2017-08-10 | método para produzir um agregado de proteína recombinante, e, agregado de proteína recombinante. |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US10899792B2 (pt) |
| EP (1) | EP3498856A4 (pt) |
| JP (2) | JP6842720B2 (pt) |
| CN (1) | CN109661472A (pt) |
| AU (1) | AU2017310785B9 (pt) |
| BR (1) | BR112019002505A2 (pt) |
| MX (1) | MX2019001751A (pt) |
| MY (1) | MY190347A (pt) |
| PH (1) | PH12019500269A1 (pt) |
| SG (1) | SG11201901093PA (pt) |
| WO (1) | WO2018030499A1 (pt) |
| ZA (1) | ZA201900661B (pt) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2020141558A (ja) * | 2017-05-24 | 2020-09-10 | Ｓｐｉｂｅｒ株式会社 | ポリペプチド溶液及びこれを製造する方法、並びにポリペプチド成形体を製造する方法 |
| JPWO2018235958A1 (ja) * | 2017-06-23 | 2020-04-23 | Ｓｐｉｂｅｒ株式会社 | タンパク質の精製方法、タンパク質溶液の製造方法、及びタンパク質成形体の製造方法 |
| CN112714813A (zh) * | 2018-09-28 | 2021-04-27 | 丝芭博株式会社 | 改性丝心蛋白纤维 |
| EP3887163A4 (en) * | 2018-11-28 | 2022-08-31 | Bolt Threads, Inc. | ALKALINE PURIFICATION OF SPIDER SILK PROTEINS |
| CN110204602B (zh) * | 2019-06-10 | 2021-11-23 | 山东农业大学 | 一种抗真菌的抗菌肽及其应用 |
| US20220378871A1 (en) * | 2019-09-30 | 2022-12-01 | Spiber Inc. | Muscle Tissue-Regenerating Agent |
| CN112574890B (zh) * | 2020-11-26 | 2022-03-15 | 浙江工业大学 | 总状毛霉sy5-47及其在桑叶黄酮提取中的应用 |
| CN112481134B (zh) * | 2020-11-26 | 2022-03-18 | 浙江工业大学 | 一种利用微生物发酵法提取桑叶多糖的方法 |
| WO2024057361A1 (ja) * | 2022-09-12 | 2024-03-21 | 興和株式会社 | 可溶化タンパク質製造方法 |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4832671B1 (pt) | 1970-10-28 | 1973-10-08 | ||
| JPS58110600A (ja) | 1981-12-25 | 1983-07-01 | Kyowa Hakko Kogyo Co Ltd | ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド |
| JPS60221091A (ja) | 1983-12-21 | 1985-11-05 | Kyowa Hakko Kogyo Co Ltd | 新規プロモ−タ− |
| JP2564268B2 (ja) | 1985-08-28 | 1996-12-18 | 協和醗酵工業株式会社 | 融合抗原ポリペプチド |
| JP2545078B2 (ja) | 1987-04-06 | 1996-10-16 | 協和醗酵工業株式会社 | 核酸関連物質の製造法 |
| EP0326046A3 (en) | 1988-01-25 | 1990-06-13 | Takeda Chemical Industries, Ltd. | Production of human epidermal growth factor |
| JP2928287B2 (ja) | 1988-09-29 | 1999-08-03 | 協和醗酵工業株式会社 | 新規ポリペプチド |
| JPH0322979A (ja) | 1989-06-19 | 1991-01-31 | Kyowa Hakko Kogyo Co Ltd | 新規プラスミノーゲン活性化因子 |
| US5235041A (en) * | 1990-12-28 | 1993-08-10 | Protein Polymer Technologies, Inc. | Purification of structurally ordered recombinant protein polymers |
| JP3012026B2 (ja) | 1991-03-05 | 2000-02-21 | ヒゲタ醤油株式会社 | 高発現ベクター及び該高発現ベクター を保有する微生物並びに該微生物を 用いる有用物質の製造法 |
| JP3433807B2 (ja) | 1992-03-31 | 2003-08-04 | 鵜高 重三 | 新規アミノ酸配列及び該アミノ酸配列をコードするｄｎａを保有する発現ベクターを組み込んだバチルス・ブレビスを用いる有用物質の製造法 |
| JP2727391B2 (ja) | 1992-07-23 | 1998-03-11 | ヒゲタ醤油株式会社 | 高発現ベクター及び該高発現ベクターを保有する微生物並びに該微生物を用いる有用物質の製造法 |
| US6620917B1 (en) | 2000-01-20 | 2003-09-16 | The United States Of America As Represented By The Secretary Of The Army | Method for the purification and aqueous fiber spinning of spider silks and other structural proteins |
| JP4491698B2 (ja) * | 2000-02-29 | 2010-06-30 | 不二製油株式会社 | 大豆蛋白加水分解物の製造方法 |
| JP3753945B2 (ja) | 2001-02-14 | 2006-03-08 | ヒゲタ醤油株式会社 | 大腸菌とブレビバチルス属細菌間のプラスミドシャトルベクター |
| TW200508393A (en) * | 2003-01-20 | 2005-03-01 | Kaneka Corp | Method of collecting highly pure polyhydroxyalkanoate from microbial cells |
| JP3716392B2 (ja) * | 2003-04-17 | 2005-11-16 | 福島県 | 野蚕または家蚕の絹フィブロイン粉末の製造方法 |
| ES2525095T3 (es) | 2004-07-22 | 2014-12-17 | Amsilk Gmbh | Proteínas recombinantes de la seda de araña |
| EP2552935B1 (en) * | 2010-03-31 | 2020-05-27 | AMSilk GmbH | Separation of insoluble target proteins |
| CN102432668A (zh) | 2011-11-25 | 2012-05-02 | 华侨大学 | 一种能对目标重组蛋白进行离心法快速分离纯化的方法 |
| EP2746390A1 (en) * | 2012-12-19 | 2014-06-25 | Sandoz Ag | Method for producing a recombinant protein of interest |
| US20150329587A1 (en) | 2012-12-27 | 2015-11-19 | Spiber Inc. | Extraction method for hydrophilic recombinant protein |
| US20150368292A1 (en) * | 2013-01-31 | 2015-12-24 | Glaxo Group Limited | Method of producing a protein |
-
2017
- 2017-08-10 AU AU2017310785A patent/AU2017310785B9/en active Active
- 2017-08-10 BR BR112019002505A patent/BR112019002505A2/pt unknown
- 2017-08-10 MX MX2019001751A patent/MX2019001751A/es unknown
- 2017-08-10 MY MYPI2019000616A patent/MY190347A/en unknown
- 2017-08-10 EP EP17839566.1A patent/EP3498856A4/en active Pending
- 2017-08-10 SG SG11201901093PA patent/SG11201901093PA/en unknown
- 2017-08-10 US US16/324,280 patent/US10899792B2/en active Active
- 2017-08-10 JP JP2018533557A patent/JP6842720B2/ja active Active
- 2017-08-10 WO PCT/JP2017/029033 patent/WO2018030499A1/ja unknown
- 2017-08-10 CN CN201780048398.8A patent/CN109661472A/zh active Pending
-
2019
- 2019-01-31 ZA ZA2019/00661A patent/ZA201900661B/en unknown
- 2019-02-07 PH PH12019500269A patent/PH12019500269A1/en unknown
-
2020
- 2020-11-17 JP JP2020191037A patent/JP7079525B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| AU2017310785A1 (en) | 2019-02-21 |
| MX2019001751A (es) | 2019-06-10 |
| JPWO2018030499A1 (ja) | 2019-06-13 |
| MY190347A (en) | 2022-04-15 |
| AU2017310785B2 (en) | 2022-06-30 |
| AU2017310785B9 (en) | 2022-07-21 |
| AU2017310785A8 (en) | 2019-03-07 |
| CN109661472A (zh) | 2019-04-19 |
| ZA201900661B (en) | 2021-08-25 |
| JP6842720B2 (ja) | 2021-03-17 |
| EP3498856A1 (en) | 2019-06-19 |
| WO2018030499A1 (ja) | 2018-02-15 |
| SG11201901093PA (en) | 2019-03-28 |
| US20190177363A1 (en) | 2019-06-13 |
| JP2021014472A (ja) | 2021-02-12 |
| EP3498856A4 (en) | 2020-05-06 |
| US10899792B2 (en) | 2021-01-26 |
| JP7079525B2 (ja) | 2022-06-02 |
| PH12019500269A1 (en) | 2019-06-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B350 | Update of information on the portal [chapter 15.35 patent gazette] | ||
| B06W | Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette] |