MX2019001751A - Metodo de produccion para agregado de proteina recombinante insoluble. - Google Patents

Metodo de produccion para agregado de proteina recombinante insoluble.

Info

Publication number
MX2019001751A
MX2019001751A MX2019001751A MX2019001751A MX2019001751A MX 2019001751 A MX2019001751 A MX 2019001751A MX 2019001751 A MX2019001751 A MX 2019001751A MX 2019001751 A MX2019001751 A MX 2019001751A MX 2019001751 A MX2019001751 A MX 2019001751A
Authority
MX
Mexico
Prior art keywords
recombinant protein
production method
protein aggregate
insoluble
insoluble recombinant
Prior art date
Application number
MX2019001751A
Other languages
English (en)
Inventor
Osawa Toshiaki
Sato Yuya
Morita Keisuke
Original Assignee
Spiber Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spiber Inc filed Critical Spiber Inc
Publication of MX2019001751A publication Critical patent/MX2019001751A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Water Supply & Treatment (AREA)
  • Insects & Arthropods (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Un objeto de la presente invención es proporcionar un método para separar con eficiencia cuerpos insolubles de una proteína recombinante, a partir de una célula recombinante que expresa una proteína recombinante objetivo, como cuerpos insolubles en la célula. La presente invención proporciona un método para producir un agregado de proteína recombinante al separar los cuerpos insolubles de una proteína recombinante a partir de una célula recombinante que expresa la proteína recombinante como cuerpos insolubles en la célula, incluyendo desestabilizar la célula recombinante, agregar los cuerpos insolubles de la proteína recombinante y separar el agregado resultante.
MX2019001751A 2016-08-10 2017-08-10 Metodo de produccion para agregado de proteina recombinante insoluble. MX2019001751A (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2016157912 2016-08-10
JP2016229227 2016-11-25
JP2017048702 2017-03-14
JP2017094144 2017-05-10
PCT/JP2017/029033 WO2018030499A1 (ja) 2016-08-10 2017-08-10 不溶性組換えタンパク質凝集体の製造方法

Publications (1)

Publication Number Publication Date
MX2019001751A true MX2019001751A (es) 2019-06-10

Family

ID=61162268

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2019001751A MX2019001751A (es) 2016-08-10 2017-08-10 Metodo de produccion para agregado de proteina recombinante insoluble.

Country Status (12)

Country Link
US (1) US10899792B2 (es)
EP (1) EP3498856A4 (es)
JP (2) JP6842720B2 (es)
CN (1) CN109661472A (es)
AU (1) AU2017310785B9 (es)
BR (1) BR112019002505A2 (es)
MX (1) MX2019001751A (es)
MY (1) MY190347A (es)
PH (1) PH12019500269A1 (es)
SG (1) SG11201901093PA (es)
WO (1) WO2018030499A1 (es)
ZA (1) ZA201900661B (es)

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* Cited by examiner, † Cited by third party
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JP2020141558A (ja) * 2017-05-24 2020-09-10 Ｓｐｉｂｅｒ株式会社 ポリペプチド溶液及びこれを製造する方法、並びにポリペプチド成形体を製造する方法
JPWO2018235958A1 (ja) * 2017-06-23 2020-04-23 Ｓｐｉｂｅｒ株式会社 タンパク質の精製方法、タンパク質溶液の製造方法、及びタンパク質成形体の製造方法
WO2020067547A1 (ja) * 2018-09-28 2020-04-02 Ｓｐｉｂｅｒ株式会社 改変フィブロイン繊維
JP2022513628A (ja) * 2018-11-28 2022-02-09 ボルト スレッズ インコーポレイテッド クモ糸タンパク質のアルカリ精製法
CN110204602B (zh) * 2019-06-10 2021-11-23 山东农业大学 一种抗真菌的抗菌肽及其应用
CN114555108A (zh) * 2019-09-30 2022-05-27 丝芭博株式会社 肌肉组织再生剂
CN112481134B (zh) * 2020-11-26 2022-03-18 浙江工业大学 一种利用微生物发酵法提取桑叶多糖的方法
CN112574890B (zh) * 2020-11-26 2022-03-15 浙江工业大学 总状毛霉sy5-47及其在桑叶黄酮提取中的应用
WO2024057361A1 (ja) * 2022-09-12 2024-03-21 興和株式会社 可溶化タンパク質製造方法

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JPS4832671B1 (es) 1970-10-28 1973-10-08
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JPS60221091A (ja) 1983-12-21 1985-11-05 Kyowa Hakko Kogyo Co Ltd 新規プロモ−タ−
JP2564268B2 (ja) 1985-08-28 1996-12-18 協和醗酵工業株式会社 融合抗原ポリペプチド
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JP3433807B2 (ja) 1992-03-31 2003-08-04 鵜高 重三 新規アミノ酸配列及び該アミノ酸配列をコードするｄｎａを保有する発現ベクターを組み込んだバチルス・ブレビスを用いる有用物質の製造法
JP2727391B2 (ja) 1992-07-23 1998-03-11 ヒゲタ醤油株式会社 高発現ベクター及び該高発現ベクターを保有する微生物並びに該微生物を用いる有用物質の製造法
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JP2016504918A (ja) 2013-01-31 2016-02-18 グラクソ グループ リミテッドＧｌａｘｏ Ｇｒｏｕｐ Ｌｉｍｉｔｅｄ タンパク質を製造する方法

Also Published As

Publication number Publication date
AU2017310785B9 (en) 2022-07-21
US10899792B2 (en) 2021-01-26
SG11201901093PA (en) 2019-03-28
US20190177363A1 (en) 2019-06-13
PH12019500269A1 (en) 2019-06-17
MY190347A (en) 2022-04-15
EP3498856A1 (en) 2019-06-19
WO2018030499A1 (ja) 2018-02-15
JPWO2018030499A1 (ja) 2019-06-13
CN109661472A (zh) 2019-04-19
AU2017310785A1 (en) 2019-02-21
BR112019002505A2 (pt) 2019-06-25
EP3498856A4 (en) 2020-05-06
ZA201900661B (en) 2021-08-25
AU2017310785A8 (en) 2019-03-07
JP7079525B2 (ja) 2022-06-02
AU2017310785B2 (en) 2022-06-30
JP2021014472A (ja) 2021-02-12
JP6842720B2 (ja) 2021-03-17

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