SG11201901093PA - Production method for insoluble recombinant protein aggregate - Google Patents

Production method for insoluble recombinant protein aggregate

Info

Publication number
SG11201901093PA
SG11201901093PA SG11201901093PA SG11201901093PA SG11201901093PA SG 11201901093P A SG11201901093P A SG 11201901093PA SG 11201901093P A SG11201901093P A SG 11201901093PA SG 11201901093P A SG11201901093P A SG 11201901093PA SG 11201901093P A SG11201901093P A SG 11201901093PA
Authority
SG
Singapore
Prior art keywords
recombinant protein
insoluble
production method
recombinant
protein aggregate
Prior art date
Application number
SG11201901093PA
Inventor
Toshiaki Osawa
Yuya Sato
Keisuke Morita
Original Assignee
Spiber Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spiber Inc filed Critical Spiber Inc
Publication of SG11201901093PA publication Critical patent/SG11201901093PA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Abstract

PRODUCTION METHOD FOR INSOLUBLE RECOMBINANT PROTEIN AGGREGATE An object of the present invention is to provide a method for 5 efficiently separating insoluble bodies of a recombinant protein from a recombinant cell expressing a target recombinant protein as insoluble bodies in the cell. The present invention provides a method for producing a recombinant protein aggregate by separating insoluble bodies of a recombinant protein from a recombinant cell expressing the 10 recombinant protein as insoluble bodies in the cell, including disrupting the recombinant cell, aggregating the insoluble bodies of the recombinant protein, and separating the resulting aggregate. Fig. 5
SG11201901093PA 2016-08-10 2017-08-10 Production method for insoluble recombinant protein aggregate SG11201901093PA (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2016157912 2016-08-10
JP2016229227 2016-11-25
JP2017048702 2017-03-14
JP2017094144 2017-05-10
PCT/JP2017/029033 WO2018030499A1 (en) 2016-08-10 2017-08-10 Production method for insoluble recombinant protein aggregate

Publications (1)

Publication Number Publication Date
SG11201901093PA true SG11201901093PA (en) 2019-03-28

Family

ID=61162268

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201901093PA SG11201901093PA (en) 2016-08-10 2017-08-10 Production method for insoluble recombinant protein aggregate

Country Status (12)

Country Link
US (1) US10899792B2 (en)
EP (1) EP3498856A4 (en)
JP (2) JP6842720B2 (en)
CN (1) CN109661472A (en)
AU (1) AU2017310785B9 (en)
BR (1) BR112019002505A2 (en)
MX (1) MX2019001751A (en)
MY (1) MY190347A (en)
PH (1) PH12019500269A1 (en)
SG (1) SG11201901093PA (en)
WO (1) WO2018030499A1 (en)
ZA (1) ZA201900661B (en)

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JP2020141558A (en) * 2017-05-24 2020-09-10 Ｓｐｉｂｅｒ株式会社 Polypeptide solution, method for producing the same, and method for producing polypeptide product
WO2018235958A1 (en) * 2017-06-23 2018-12-27 Ｓｐｉｂｅｒ株式会社 Method for purifying protein, method for producing protein solution, and method for producing protein moulded body
CN112714813A (en) * 2018-09-28 2021-04-27 丝芭博株式会社 Modified fibroin fibers
WO2020112742A1 (en) * 2018-11-28 2020-06-04 Bolt Threads, Inc. Alkaline purification of spider silk proteins
CN110204602B (en) * 2019-06-10 2021-11-23 山东农业大学 Antifungal antibacterial peptide and application thereof
JPWO2021066078A1 (en) * 2019-09-30 2021-04-08
CN112574890B (en) * 2020-11-26 2022-03-15 浙江工业大学 Mucor racemosus SY5-47 and application thereof in mulberry leaf flavone extraction
CN112481134B (en) * 2020-11-26 2022-03-18 浙江工业大学 Method for extracting mulberry leaf polysaccharide by using microbial fermentation method
WO2024057361A1 (en) * 2022-09-12 2024-03-21 興和株式会社 Solubilized protein production method

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Also Published As

Publication number Publication date
EP3498856A4 (en) 2020-05-06
US20190177363A1 (en) 2019-06-13
JP7079525B2 (en) 2022-06-02
JP2021014472A (en) 2021-02-12
AU2017310785B9 (en) 2022-07-21
CN109661472A (en) 2019-04-19
JPWO2018030499A1 (en) 2019-06-13
BR112019002505A2 (en) 2019-06-25
AU2017310785A1 (en) 2019-02-21
ZA201900661B (en) 2021-08-25
MX2019001751A (en) 2019-06-10
US10899792B2 (en) 2021-01-26
AU2017310785B2 (en) 2022-06-30
PH12019500269A1 (en) 2019-06-17
WO2018030499A1 (en) 2018-02-15
MY190347A (en) 2022-04-15
JP6842720B2 (en) 2021-03-17
EP3498856A1 (en) 2019-06-19
AU2017310785A8 (en) 2019-03-07

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