BR102014027448A8 - cassete de doador de polinucleotídeo, uso de uma planta, de uma parte de planta, ou de uma célula vegetal compreendendo o referido cassete, e método para integração direcionada do mesmo - Google Patents

cassete de doador de polinucleotídeo, uso de uma planta, de uma parte de planta, ou de uma célula vegetal compreendendo o referido cassete, e método para integração direcionada do mesmo

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Publication number
BR102014027448A8
BR102014027448A8 BR102014027448A BR102014027448A BR102014027448A8 BR 102014027448 A8 BR102014027448 A8 BR 102014027448A8 BR 102014027448 A BR102014027448 A BR 102014027448A BR 102014027448 A BR102014027448 A BR 102014027448A BR 102014027448 A8 BR102014027448 A8 BR 102014027448A8
Authority
BR
Brazil
Prior art keywords
plant
cassette
targeted integration
polynucleotide donor
cell
Prior art date
Application number
BR102014027448A
Other languages
English (en)
Other versions
BR102014027448A2 (pt
Inventor
Sastry-Dent Lakshmi
C Blue Ryan
L Evans Steven
Cao Zehui
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of BR102014027448A2 publication Critical patent/BR102014027448A2/pt
Publication of BR102014027448A8 publication Critical patent/BR102014027448A8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate

Abstract

sistema doador universal para direcionamento de gene. um polinucleotídeo doador universal é descrito, que pode ser inserido em localizações alvos em genomas de planta para facilitar integração de produtividade rápida e alta de uma molécula doadora dentro de uma localização genômica específica.
BR102014027448A 2013-11-04 2014-11-03 cassete de doador de polinucleotídeo, uso de uma planta, de uma parte de planta, ou de uma célula vegetal compreendendo o referido cassete, e método para integração direcionada do mesmo BR102014027448A8 (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361899543P 2013-11-04 2013-11-04

Publications (2)

Publication Number Publication Date
BR102014027448A2 BR102014027448A2 (pt) 2015-09-15
BR102014027448A8 true BR102014027448A8 (pt) 2021-08-24

Family

ID=53005271

Family Applications (1)

Application Number Title Priority Date Filing Date
BR102014027448A BR102014027448A8 (pt) 2013-11-04 2014-11-03 cassete de doador de polinucleotídeo, uso de uma planta, de uma parte de planta, ou de uma célula vegetal compreendendo o referido cassete, e método para integração direcionada do mesmo

Country Status (14)

Country Link
US (1) US10077449B2 (pt)
EP (1) EP3066192B1 (pt)
JP (1) JP6560203B2 (pt)
KR (1) KR102248730B1 (pt)
CN (1) CN105683357B (pt)
AR (1) AR098283A1 (pt)
AU (1) AU2014341928B2 (pt)
BR (1) BR102014027448A8 (pt)
CA (1) CA2926534A1 (pt)
IL (1) IL245313B (pt)
RU (1) RU2687369C2 (pt)
TW (1) TWI669395B (pt)
WO (1) WO2015066637A1 (pt)
ZA (1) ZA201601929B (pt)

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Also Published As

Publication number Publication date
KR20160079090A (ko) 2016-07-05
US20150143588A1 (en) 2015-05-21
IL245313B (en) 2019-05-30
CA2926534A1 (en) 2015-05-07
RU2016122038A3 (pt) 2018-06-21
TW201522630A (zh) 2015-06-16
WO2015066637A1 (en) 2015-05-07
RU2687369C2 (ru) 2019-05-13
KR102248730B1 (ko) 2021-05-07
ZA201601929B (en) 2019-06-26
CN105683357B (zh) 2020-10-30
JP2016534727A (ja) 2016-11-10
AU2014341928A1 (en) 2016-04-07
EP3066192A4 (en) 2017-07-19
BR102014027448A2 (pt) 2015-09-15
JP6560203B2 (ja) 2019-08-14
AR098283A1 (es) 2016-05-26
EP3066192A1 (en) 2016-09-14
TWI669395B (zh) 2019-08-21
CN105683357A (zh) 2016-06-15
AU2014341928B2 (en) 2017-11-30
US10077449B2 (en) 2018-09-18
EP3066192B1 (en) 2019-05-01
IL245313A0 (en) 2016-06-30
RU2016122038A (ru) 2017-12-11

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