AR101080A1 - Transformación de maíz haploide con una nucleasa específica de sitio - Google Patents
Transformación de maíz haploide con una nucleasa específica de sitioInfo
- Publication number
- AR101080A1 AR101080A1 ARP150101285A ARP150101285A AR101080A1 AR 101080 A1 AR101080 A1 AR 101080A1 AR P150101285 A ARP150101285 A AR P150101285A AR P150101285 A ARP150101285 A AR P150101285A AR 101080 A1 AR101080 A1 AR 101080A1
- Authority
- AR
- Argentina
- Prior art keywords
- haploid
- tissue
- genome
- derived
- specific nuclease
- Prior art date
Links
- 101710163270 Nuclease Proteins 0.000 title abstract 4
- 240000008042 Zea mays Species 0.000 title abstract 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title abstract 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 title abstract 4
- 235000005822 corn Nutrition 0.000 title abstract 4
- 230000009466 transformation Effects 0.000 title abstract 4
- 238000000034 method Methods 0.000 abstract 5
- 241000243190 Microsporidia Species 0.000 abstract 2
- 230000001548 androgenic effect Effects 0.000 abstract 2
- 210000003783 haploid cell Anatomy 0.000 abstract 2
- 230000035772 mutation Effects 0.000 abstract 2
- 241000196324 Embryophyta Species 0.000 abstract 1
- 230000010354 integration Effects 0.000 abstract 1
- 238000004161 plant tissue culture Methods 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 230000001172 regenerating effect Effects 0.000 abstract 1
- 230000008685 targeting Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Se describen métodos para la transformación de una línea celular haploide derivada androgénica con una nucleasa específica del sitio. En algunas formas de realización, la línea celular haploide derivada androgénica es un cultivo de tejido de planta derivado de microspora de maíz. Además, la descripción proporciona un método para modificar, por ejemplo, mediante la mutación o direccionamiento e integración del DNA donante en, un locus específico de un genoma del tejido haploide o dihaploide. La descripción también proporciona métodos para regenerar una planta entera del tejido haploide o dihaploide que contiene la mutación en un locus genómico específico o un ADN donante integrante dentro de un locus genómico específico se puede obtener de la presente descripción. Reivindicación 1: Un método para modificar un genoma de maíz, el método que comprende: (a) proporcionar el tejido haploide competente para transformación derivado de la microspora de maíz que comprende un genoma del tejido haploide; (b) administrar un polinucleótido que codifica una nucleasa especifica del sitio en el tejido haploide competente para transformación; y (c) confirmar que el genoma del tejido haploide está modificado por la nucleasa específica del sitio codificada.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461985042P | 2014-04-28 | 2014-04-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR101080A1 true AR101080A1 (es) | 2016-11-23 |
Family
ID=54334185
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP150101285A AR101080A1 (es) | 2014-04-28 | 2015-04-28 | Transformación de maíz haploide con una nucleasa específica de sitio |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US20150307889A1 (es) |
| EP (1) | EP3136842A4 (es) |
| JP (1) | JP2017513506A (es) |
| KR (1) | KR20160145649A (es) |
| CN (1) | CN106455512A (es) |
| AR (1) | AR101080A1 (es) |
| AU (1) | AU2015253536A1 (es) |
| BR (1) | BR102015009386A2 (es) |
| CA (1) | CA2946987A1 (es) |
| IL (1) | IL248456A0 (es) |
| MX (1) | MX2016013982A (es) |
| RU (1) | RU2016143352A (es) |
| TW (1) | TW201546283A (es) |
| WO (1) | WO2015167956A1 (es) |
Families Citing this family (61)
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| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
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| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US20150166984A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting alpha-antitrypsin point mutations |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| EP3316676A4 (en) | 2015-06-30 | 2018-12-12 | Regents of the University of Minnesota | Haploid inducer line for accelerated genome editing |
| SG10202104041PA (en) | 2015-10-23 | 2021-06-29 | Harvard College | Nucleobase editors and uses thereof |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| UY40584A (es) | 2016-08-17 | 2024-01-15 | Monsanto Technology Llc | MÉTODOS, COMPOSICIONES y PLANTAS MEDIANTE LA MANIPULACIÓN DEL METABOLISMO DE GIBERELINA PARA AUMENTAR EL RENDIMIENTO COSECHABLE |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| SG11201903089RA (en) | 2016-10-14 | 2019-05-30 | Harvard College | Aav delivery of nucleobase editors |
| CA3042259A1 (en) | 2016-11-04 | 2018-05-11 | Flagship Pioneering Innovations V. Inc. | Novel plant cells, plants, and seeds |
| KR102720045B1 (ko) * | 2016-12-02 | 2024-10-18 | 신젠타 파티서페이션즈 아게 | 동시 유전자 편집 및 반수체 유도 |
| US10519456B2 (en) | 2016-12-02 | 2019-12-31 | Syngenta Participations Ag | Simultaneous gene editing and haploid induction |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| SG11201906795SA (en) * | 2017-01-28 | 2019-08-27 | Inari Agriculture Inc | Novel plant cells, plants, and seeds |
| US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| EP3592381A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Cancer vaccine |
| WO2018165629A1 (en) | 2017-03-10 | 2018-09-13 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| WO2018176009A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| CN107083395A (zh) * | 2017-04-11 | 2017-08-22 | 未名兴旺系统作物设计前沿实验室（北京）有限公司 | 提高小麦幼胚基因枪瞬时转化效率的方法和培养基配方 |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| CN107034233B (zh) * | 2017-05-18 | 2021-06-08 | 中国科学院重庆绿色智能技术研究院 | 一种内源性启动子驱动外源基因表达的方法 |
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| US11603536B2 (en) | 2017-09-29 | 2023-03-14 | Inari Agriculture Technology, Inc. | Methods for efficient maize genome editing |
| US11795443B2 (en) | 2017-10-16 | 2023-10-24 | The Broad Institute, Inc. | Uses of adenosine base editors |
| WO2019118949A1 (en) | 2017-12-15 | 2019-06-20 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US11802288B1 (en) | 2018-01-29 | 2023-10-31 | Inari Agriculture Technology, Inc. | Methods for efficient soybean genome editing |
| US11220694B1 (en) | 2018-01-29 | 2022-01-11 | Inari Agriculture, Inc. | Rice cells and rice plants |
| US11926835B1 (en) | 2018-01-29 | 2024-03-12 | Inari Agriculture Technology, Inc. | Methods for efficient tomato genome editing |
| WO2019161143A1 (en) | 2018-02-15 | 2019-08-22 | Monsanto Technology Llc | Improved methods for hybrid corn seed production |
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| WO2019234132A1 (en) * | 2018-06-05 | 2019-12-12 | KWS SAAT SE & Co. KGaA | Base editing in polymerase theta deficient plants |
| WO2019234129A1 (en) * | 2018-06-05 | 2019-12-12 | KWS SAAT SE & Co. KGaA | Haploid induction with modified dna-repair |
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| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
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| US12404515B2 (en) * | 2018-12-21 | 2025-09-02 | Syngenta Crop Protection Ag | Simultaneous gene editing and haploid induction |
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| EP3942043A2 (en) | 2019-03-19 | 2022-01-26 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| WO2020214842A1 (en) | 2019-04-17 | 2020-10-22 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12543674B2 (en) | 2019-04-18 | 2026-02-10 | Pioneer Hi-Bred International, Inc. | Embryogenesis factors for cellular reprogramming of a plant cell |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| CN111471788B (zh) * | 2020-04-15 | 2022-09-13 | 中国农业科学院作物科学研究所 | 转SbSNAC1基因玉米SbSNAC1-466的外源插入片段的旁侧序列及其应用 |
| BR112022022603A2 (pt) | 2020-05-08 | 2023-01-17 | Broad Inst Inc | Métodos e composições para edição simultânea de ambas as fitas de sequência alvo de nucleotídeos de fita dupla |
| US20220159905A1 (en) | 2020-11-23 | 2022-05-26 | Monsanto Technology Llc | Management of corn through semi-dwarf systems |
| US12577624B2 (en) | 2021-11-02 | 2026-03-17 | Monsanto Technology Llc | Transgenic corn event ZM_BCS216090 and methods for detection and uses thereof |
| CN115067208B (zh) * | 2022-07-21 | 2023-05-16 | 北京市农林科学院 | 一种利用赤霉素提高玉米单倍体加倍效率的方法 |
| CN116267583B (zh) * | 2023-03-15 | 2024-07-30 | 北京市农林科学院 | 一种玉米优异性状精准导入与鉴定方法 |
| CN116602212B (zh) * | 2023-06-06 | 2024-05-03 | 北京市农林科学院 | 芍药属植物离体培养和增殖方法及专用培养基 |
| WO2025025203A1 (en) * | 2023-08-03 | 2025-02-06 | Syngenta Crop Protection Ag | Zygote-preferred expression |
| CN118667877B (zh) * | 2024-08-23 | 2024-10-29 | 河南科技大学 | 发根农杆菌介导非组培依赖的芍药根系遗传转化种植方法 |
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| US5306864A (en) * | 1988-12-02 | 1994-04-26 | United Agriseeds | Increasing the anther culturability of maize |
| US20020023278A1 (en) * | 2000-05-08 | 2002-02-21 | Lyznik Leszek Alexander | Genetic transformation in plants using site-specific recombination and wide hybridization |
| US20020188965A1 (en) * | 2001-04-20 | 2002-12-12 | Zou-Yu Zhao | Methods of transforming plants |
| US8796508B2 (en) * | 2005-11-10 | 2014-08-05 | Pioneer Hi-Bred International, Inc. | Microprojectile bombardment transformation of Brassica |
| AU2005338055B2 (en) * | 2005-11-10 | 2012-02-02 | Pioneer Hi-Bred International, Inc. | Microprojectile bombardment transformation of Brassica |
| CN101680000B (zh) * | 2007-06-11 | 2014-06-11 | 拜尔作物科学公司 | 包含优良事件ee-gh6的抗虫棉花植物及其鉴定方法 |
| BRPI0816750A2 (pt) * | 2007-06-29 | 2015-09-29 | Pioneer Hi Bred Int | métodos para alterar o genoma de uma célula de planta monocotiledônea e para modificar uma sequência alvo genômica endógena específica e planta de milho |
| US20110203012A1 (en) * | 2010-01-21 | 2011-08-18 | Dotson Stanton B | Methods and compositions for use of directed recombination in plant breeding |
| EP2612918A1 (en) * | 2012-01-06 | 2013-07-10 | BASF Plant Science Company GmbH | In planta recombination |
| JP6559063B2 (ja) * | 2012-05-07 | 2019-08-14 | サンガモ セラピューティクス， インコーポレイテッド | 導入遺伝子のヌクレアーゼ媒介標的化組み込みのための方法および組成物 |
-
2015
- 2015-04-24 RU RU2016143352A patent/RU2016143352A/ru not_active Application Discontinuation
- 2015-04-24 CN CN201580024275.1A patent/CN106455512A/zh active Pending
- 2015-04-24 WO PCT/US2015/027484 patent/WO2015167956A1/en not_active Ceased
- 2015-04-24 MX MX2016013982A patent/MX2016013982A/es unknown
- 2015-04-24 KR KR1020167031047A patent/KR20160145649A/ko not_active Withdrawn
- 2015-04-24 JP JP2016564295A patent/JP2017513506A/ja active Pending
- 2015-04-24 AU AU2015253536A patent/AU2015253536A1/en not_active Abandoned
- 2015-04-24 CA CA2946987A patent/CA2946987A1/en active Pending
- 2015-04-24 EP EP15785964.6A patent/EP3136842A4/en not_active Withdrawn
- 2015-04-24 US US14/695,434 patent/US20150307889A1/en active Pending
- 2015-04-27 BR BR102015009386A patent/BR102015009386A2/pt not_active Application Discontinuation
- 2015-04-27 TW TW104113391A patent/TW201546283A/zh unknown
- 2015-04-28 AR ARP150101285A patent/AR101080A1/es unknown
-
2016
- 2016-10-23 IL IL248456A patent/IL248456A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU2015253536A1 (en) | 2016-11-17 |
| WO2015167956A1 (en) | 2015-11-05 |
| JP2017513506A (ja) | 2017-06-01 |
| EP3136842A1 (en) | 2017-03-08 |
| TW201546283A (zh) | 2015-12-16 |
| EP3136842A4 (en) | 2017-11-29 |
| CN106455512A (zh) | 2017-02-22 |
| MX2016013982A (es) | 2017-01-11 |
| CA2946987A1 (en) | 2015-11-05 |
| RU2016143352A (ru) | 2018-05-28 |
| US20150307889A1 (en) | 2015-10-29 |
| BR102015009386A2 (pt) | 2016-01-05 |
| KR20160145649A (ko) | 2016-12-20 |
| IL248456A0 (en) | 2016-11-30 |
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