MX2022010832A - Modificacion del sitio de enlace represor transcripcional en el promotor nf-yc4 para contenido de proteina incrementado y resistencia al estres. - Google Patents

Modificacion del sitio de enlace represor transcripcional en el promotor nf-yc4 para contenido de proteina incrementado y resistencia al estres.

Info

Publication number
MX2022010832A
MX2022010832A MX2022010832A MX2022010832A MX2022010832A MX 2022010832 A MX2022010832 A MX 2022010832A MX 2022010832 A MX2022010832 A MX 2022010832A MX 2022010832 A MX2022010832 A MX 2022010832A MX 2022010832 A MX2022010832 A MX 2022010832A
Authority
MX
Mexico
Prior art keywords
transcriptional repressor
binding site
protein content
resistance
repressor binding
Prior art date
Application number
MX2022010832A
Other languages
English (en)
Inventor
Ling Li
Eve Syrkin Wurtele
Original Assignee
Univ Iowa State Res Found Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Iowa State Res Found Inc filed Critical Univ Iowa State Res Found Inc
Publication of MX2022010832A publication Critical patent/MX2022010832A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
    • A01H1/122—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4636—Oryza sp. [rice]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
    • A01H6/542—Glycine max [soybean]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Pest Control & Pesticides (AREA)
  • Insects & Arthropods (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Método para incrementar el contenido de proteína en una célula eucariótica que comprende un gen NF-YC4 que comprende la modificación del sitio de enlace represor transcripcional; método para producir una planta con contenido de proteína incrementado que comprende el cruzamiento y selección para el contenido de proteína incrementado; método para incrementar la resistencia a un patógeno o a una plaga en una planta que comprende un gen NF-YC4 que comprende modificar el sitio de enlace represor transcripcional, solo o en combinación adicional con la expresión de QQS en la planta; método para producir una planta con resistencia incrementada a un patógeno o a una plaga que comprende cruzar y seleccionar para la resistencia incrementada al patógeno o a la plaga; una célula, colección de células, tejido, órgano, u organismo en el cual el gen NF-YC4 comprende un promotor que comprende un sitio de enlace represor transcripcional que se ha modificado de modo que el represor transcripcional no puede prevenir la transcripción del NF-YC4; plantas híbridas y semillas.
MX2022010832A 2015-02-18 2017-08-14 Modificacion del sitio de enlace represor transcripcional en el promotor nf-yc4 para contenido de proteina incrementado y resistencia al estres. MX2022010832A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562117924P 2015-02-18 2015-02-18
US201562244131P 2015-10-20 2015-10-20

Publications (1)

Publication Number Publication Date
MX2022010832A true MX2022010832A (es) 2022-09-29

Family

ID=55587336

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2017010480A MX2017010480A (es) 2015-02-18 2016-02-17 Modificacion del sitio de enlace represor transcripcional en el promotor nf-yc4 para contenido de proteina incrementado y resistencia al estres.
MX2022010832A MX2022010832A (es) 2015-02-18 2017-08-14 Modificacion del sitio de enlace represor transcripcional en el promotor nf-yc4 para contenido de proteina incrementado y resistencia al estres.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2017010480A MX2017010480A (es) 2015-02-18 2016-02-17 Modificacion del sitio de enlace represor transcripcional en el promotor nf-yc4 para contenido de proteina incrementado y resistencia al estres.

Country Status (14)

Country Link
US (3) US10640781B2 (es)
EP (1) EP3258774A1 (es)
JP (3) JP6785235B2 (es)
KR (2) KR20240014627A (es)
CN (2) CN114990070A (es)
AU (2) AU2016220088C1 (es)
BR (2) BR122023024788A2 (es)
CA (1) CA2977215A1 (es)
CL (1) CL2017002092A1 (es)
HK (1) HK1248274A1 (es)
IL (2) IL296837A (es)
MX (2) MX2017010480A (es)
WO (1) WO2016134081A1 (es)
ZA (1) ZA201705045B (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR122023024788A2 (pt) * 2015-02-18 2023-12-26 Iowa State University Research Foundation, Inc. Genoma de planta geneticamente modificado para aumentar o teor de proteína e resistência a um agente patogênico ou a uma praga
KR20200103769A (ko) * 2018-01-23 2020-09-02 기초과학연구원 연장된 단일 가이드 rna 및 그 용도
CN111988989A (zh) * 2018-04-18 2020-11-24 先锋国际良种公司 通过修饰内源性mads盒转录因子改善玉蜀黍中的农艺特征
CN110894505A (zh) * 2018-08-23 2020-03-20 中国农业科学院作物科学研究所 一种培育抗大豆花叶病毒的大豆的方法
KR101994831B1 (ko) * 2018-10-02 2019-07-01 주식회사 바이오메딕 감귤 트리스테자 바이러스 저항성 유전자좌의 유전형 결정용 멀티플렉스 프라이머 세트 및 이의 용도
WO2020092491A1 (en) * 2018-10-31 2020-05-07 Pioneer Hi-Bred International, Inc. Genome editing to increase seed protein content
US20230104317A1 (en) * 2020-01-31 2023-04-06 The Board Of Trustees Of The Leland Stanford Junior University Systems and Methods to Identify Genetic Silencers and Applications Thereof
CN112301038B (zh) * 2020-10-26 2023-06-13 吉林农业大学 人参wrky64-04基因及其应用
JPWO2022172851A1 (es) 2021-02-10 2022-08-18
CN113528518B (zh) * 2021-06-28 2022-07-15 西南大学 抑制核盘菌的miRNA及其应用
WO2023230631A1 (en) 2022-05-27 2023-11-30 Roger Paul Hellens Novel methods for identification and use of upstream open reading frames
CN116790634B (zh) * 2023-06-19 2024-04-16 昆明理工大学 一种锌指转录因子基因及其应用

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5618988A (en) 1990-03-02 1997-04-08 Amoco Corporation Enhanced carotenoid accumulation in storage organs of genetically engineered plants
US5837848A (en) 1990-03-16 1998-11-17 Zeneca Limited Root-specific promoter
IL101508A0 (en) 1991-04-08 1992-12-30 Rhone Poulenc Agrochimie Chimeric plant genes based on upstream regulatory elements of helianthinin
US8030546B2 (en) 1998-09-22 2011-10-04 Mendel Biotechnology, Inc. Biotic and abiotic stress tolerance in plants
US20050086718A1 (en) 1999-03-23 2005-04-21 Mendel Biotechnology, Inc. Plant transcriptional regulators of abiotic stress
CN1836045B (zh) 2003-03-28 2012-05-09 孟山都技术有限公司 用于早期种子发育的新型植物启动子
JP2005304409A (ja) 2004-04-23 2005-11-04 Yamasa Shoyu Co Ltd 表皮に傷を付けた丸大豆及びその利用
US7632982B2 (en) 2006-06-23 2009-12-15 Monsanto Technology Llc Drought responsive promoters HVA22e and PLDδ identified from Arabidopsis thaliana
US8927811B2 (en) * 2006-08-07 2015-01-06 Mendel Biotechnology, Inc. Plants with enhanced size and growth rate
EP1917858A1 (en) 2006-09-29 2008-05-07 Syngeta Participations AG A method for enhancing plant tolerance
JP5735775B2 (ja) * 2009-10-21 2015-06-17 花王株式会社 改変プロモーター
US10415053B2 (en) * 2011-02-24 2019-09-17 Iowa State University Research Foundation, Inc. Materials and method for increasing a plant's resistance to stress
US9157091B2 (en) 2011-02-24 2015-10-13 Iowa State University Research Foundation, Inc. Materials and method for modifying a biochemical component in a plant
CN103509094B (zh) * 2012-06-25 2015-09-02 中国农业科学院作物科学研究所 植物耐逆性相关蛋白GmNF-YC9及其编码基因与应用
US20140186957A1 (en) * 2013-01-03 2014-07-03 Fred Hutchinson Cancer Research Center Engineered tal effector proteins with enhanced dna targeting capacity
KR102210319B1 (ko) * 2013-03-15 2021-02-01 더 제너럴 하스피탈 코포레이션 특정 게놈 좌위에 대한 유전적 및 후성적 조절 단백질의 rna-안내 표적화
BR122023024788A2 (pt) 2015-02-18 2023-12-26 Iowa State University Research Foundation, Inc. Genoma de planta geneticamente modificado para aumentar o teor de proteína e resistência a um agente patogênico ou a uma praga
CN112795572B (zh) * 2020-11-13 2022-12-27 中国农业大学 月季核因子RhNF-YC9及其在调控花瓣扩展中的应用
US20230151378A1 (en) * 2021-10-28 2023-05-18 Board Of Trustees Of Southern Illinois University Soybean Lines with High Seed Protein and Steady to High Oil Content

Also Published As

Publication number Publication date
IL254038B (en) 2022-11-01
JP2022058741A (ja) 2022-04-12
BR122023024788A2 (pt) 2023-12-26
MX2017010480A (es) 2017-11-13
AU2022201189A1 (en) 2022-03-17
IL296837A (en) 2022-11-01
US20230139093A1 (en) 2023-05-04
CN107532148A (zh) 2018-01-02
AU2016220088C1 (en) 2022-12-08
WO2016134081A1 (en) 2016-08-25
IL254038A0 (en) 2017-10-31
KR20240014627A (ko) 2024-02-01
AU2016220088A1 (en) 2017-09-07
CN107532148B (zh) 2022-06-24
US11542520B2 (en) 2023-01-03
JP7038177B2 (ja) 2022-03-17
JP2021019627A (ja) 2021-02-18
CL2017002092A1 (es) 2018-05-18
HK1248274A1 (zh) 2018-10-12
KR20170121218A (ko) 2017-11-01
AU2016220088B2 (en) 2021-11-25
KR102630802B1 (ko) 2024-01-31
IL254038B2 (en) 2023-03-01
JP6785235B2 (ja) 2020-11-18
US20200332309A1 (en) 2020-10-22
JP2018509898A (ja) 2018-04-12
ZA201705045B (en) 2022-05-25
US10640781B2 (en) 2020-05-05
BR112017017798A2 (pt) 2018-04-10
JP7453999B2 (ja) 2024-03-21
CN114990070A (zh) 2022-09-02
EP3258774A1 (en) 2017-12-27
US20180030465A1 (en) 2018-02-01
CA2977215A1 (en) 2016-08-25

Similar Documents

Publication Publication Date Title
MX2022010832A (es) Modificacion del sitio de enlace represor transcripcional en el promotor nf-yc4 para contenido de proteina incrementado y resistencia al estres.
MX2018004809A (es) Metodos y composiciones para la modificacion genomica exenta de marcadores.
WO2016012544A3 (en) Enhanced reprogramming to ips cells
MX2022015603A (es) Expresion transgenica selectiva de tejidos.
PH12017500650A1 (en) Copi coatomer gamma subunit nucleic acid molecules that confer resistance to coleopteran and hemipteran pests
AR101080A1 (es) Transformación de maíz haploide con una nucleasa específica de sitio
EP3229586A4 (en) Genetically modified cells, tissues, and organs for treating disease
PH12017500056A1 (en) Agricultural methods
GB2561496A (en) Devices and methods for simulating a function of a liver tissue
GB2562396A (en) Devices for simulating a function of a liver tissue and methods of use and manufacturing thereof
WO2010054348A3 (en) Pathogen-inducible promoters and their use in enhancing the disease resistance of plants
MX2017009039A (es) Genes de determinación del sexo y su uso en el cultivo.
WO2012098537A8 (en) Nucleic acid construct for expression of alpha-galactosidase in plants and plant cells
EP3188739A4 (en) Automated, multifunctional, engineered cardiac tissue culture and testing bioreactor system
AR082510A1 (es) Metodo para aumentar la resistencia a infecciones fungicas en plantas transgenicas mediante el gen hcp-2
EP3489351A4 (en) CELL CULTIVATION METHOD, SUSPENDED CELL ELIMINATION METHOD AND METHOD FOR KILLING SUSPENDED CELLS
MX2016014313A (es) Moleculas de acido nucleico dre4 que confieren resistencia a las plagas de coleopteros.
MX2017007904A (es) Control biologico de plagas de coleopteros.
WO2015073625A3 (en) Synergistic genome-nonintegrating reprogramming by micrornas and transcription factors
EP4269600A3 (en) Methods of modulating seed and organ size in plants
PH12016502024A1 (en) Tonoplast proton/sugar antiporter proteins and the use thereof to increase the saccharose concentration in a saccharose storage organ of plants
PH12016501447A1 (en) Zea mays regulatory elements and uses thereof
PH12017500695A1 (en) Herbicide tolerance genes and methods of use thereof
WO2014064705A3 (en) Transgenic plants exhibiting improved resistance to abiotic stress
CR20120246A (es) Método de cultivo de viñedos y obtención de sus levaduras para fermentación en altos contenidos de azúcar y alcohol