MX2022010832A - Modificacion del sitio de enlace represor transcripcional en el promotor nf-yc4 para contenido de proteina incrementado y resistencia al estres. - Google Patents

Modificacion del sitio de enlace represor transcripcional en el promotor nf-yc4 para contenido de proteina incrementado y resistencia al estres.

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Publication number
MX2022010832A
MX2022010832A MX2022010832A MX2022010832A MX2022010832A MX 2022010832 A MX2022010832 A MX 2022010832A MX 2022010832 A MX2022010832 A MX 2022010832A MX 2022010832 A MX2022010832 A MX 2022010832A MX 2022010832 A MX2022010832 A MX 2022010832A
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Mexico
Prior art keywords
transcriptional repressor
binding site
protein content
resistance
repressor binding
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Application number
MX2022010832A
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English (en)
Inventor
Ling Li
Eve Syrkin Wurtele
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Univ Iowa State Res Found Inc
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Publication date
Application filed by Univ Iowa State Res Found Inc filed Critical Univ Iowa State Res Found Inc
Publication of MX2022010832A publication Critical patent/MX2022010832A/es

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Abstract

Método para incrementar el contenido de proteína en una célula eucariótica que comprende un gen NF-YC4 que comprende la modificación del sitio de enlace represor transcripcional; método para producir una planta con contenido de proteína incrementado que comprende el cruzamiento y selección para el contenido de proteína incrementado; método para incrementar la resistencia a un patógeno o a una plaga en una planta que comprende un gen NF-YC4 que comprende modificar el sitio de enlace represor transcripcional, solo o en combinación adicional con la expresión de QQS en la planta; método para producir una planta con resistencia incrementada a un patógeno o a una plaga que comprende cruzar y seleccionar para la resistencia incrementada al patógeno o a la plaga; una célula, colección de células, tejido, órgano, u organismo en el cual el gen NF-YC4 comprende un promotor que comprende un sitio de enlace represor transcripcional que se ha modificado de modo que el represor transcripcional no puede prevenir la transcripción del NF-YC4; plantas híbridas y semillas.
MX2022010832A 2015-02-18 2017-08-14 Modificacion del sitio de enlace represor transcripcional en el promotor nf-yc4 para contenido de proteina incrementado y resistencia al estres. MX2022010832A (es)

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US201562117924P 2015-02-18 2015-02-18
US201562244131P 2015-10-20 2015-10-20

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MX2017010480A MX395286B (es) 2015-02-18 2016-02-17 Modificacion del sitio de enlace represor transcripcional en el promotor nf-yc4 para contenido de proteina incrementado y resistencia al estres.
MX2022010832A MX2022010832A (es) 2015-02-18 2017-08-14 Modificacion del sitio de enlace represor transcripcional en el promotor nf-yc4 para contenido de proteina incrementado y resistencia al estres.

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US (4) US10640781B2 (es)
EP (1) EP3258774A1 (es)
JP (3) JP6785235B2 (es)
KR (2) KR102630802B1 (es)
CN (2) CN114990070B (es)
AU (2) AU2016220088C1 (es)
BR (1) BR122023024788A2 (es)
CA (1) CA2977215A1 (es)
CL (1) CL2017002092A1 (es)
HK (1) HK1248274A1 (es)
IL (2) IL296837A (es)
MX (2) MX395286B (es)
WO (1) WO2016134081A1 (es)
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Publication number Priority date Publication date Assignee Title
AU2016220088C1 (en) 2015-02-18 2022-12-08 Iowa State University Research Foundation, Inc. Modification of transcriptional repressor binding site in NF-YC4 promoter for increased protein content and resistance to stress
CN111742051A (zh) * 2018-01-23 2020-10-02 基础科学研究院 延伸的单向导rna及其用途
US12371702B2 (en) 2018-04-18 2025-07-29 Pioneer Hi-Bred International, Inc. Improving agronomic characteristics in maize by modification of endogenous mads box transcription factors
AU2019255192B2 (en) 2018-04-18 2023-02-02 Pioneer Hi-Bred International, Inc. Genes, constructs and maize event DP-202216-6
CN110894505A (zh) * 2018-08-23 2020-03-20 中国农业科学院作物科学研究所 一种培育抗大豆花叶病毒的大豆的方法
KR101994831B1 (ko) * 2018-10-02 2019-07-01 주식회사 바이오메딕 감귤 트리스테자 바이러스 저항성 유전자좌의 유전형 결정용 멀티플렉스 프라이머 세트 및 이의 용도
CA3114913A1 (en) * 2018-10-31 2020-05-07 Pioneer Hi-Bred International, Inc. Genome editing to increase seed protein content
US20230104317A1 (en) * 2020-01-31 2023-04-06 The Board Of Trustees Of The Leland Stanford Junior University Systems and Methods to Identify Genetic Silencers and Applications Thereof
CN112301038B (zh) * 2020-10-26 2023-06-13 吉林农业大学 人参wrky64-04基因及其应用
WO2022172851A1 (ja) 2021-02-10 2022-08-18 株式会社エンビジョンAescジャパン 電池
CN113528518B (zh) * 2021-06-28 2022-07-15 西南大学 抑制核盘菌的miRNA及其应用
EP4532734A1 (en) 2022-05-27 2025-04-09 Genxtraits, Inc. Novel methods for identification and use of upstream open reading frames
CN116790634B (zh) * 2023-06-19 2024-04-16 昆明理工大学 一种锌指转录因子基因及其应用
CN118421693B (zh) * 2024-04-25 2024-12-10 广东省农业科学院设施农业研究所 CaCP450基因对黄瓜疫病的抗性及应用
CN118460595B (zh) * 2024-05-17 2026-02-13 中国农业大学 紫花苜蓿MsNF-YC4基因提高植物耐逆性的应用
CN119372214B (zh) * 2024-11-22 2025-09-16 南京林业大学 毛竹PeTEM1基因及其表达蛋白和应用
CN119639764A (zh) * 2024-12-13 2025-03-18 扬州大学 一种草莓抗逆及果品调控基因FaCOL1及其应用
CN120866352A (zh) * 2025-08-29 2025-10-31 湖北省农业科学院果树茶叶研究所 枳PtNF-YA3基因及其在植物抗旱遗传改良中的应用

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5618988A (en) 1990-03-02 1997-04-08 Amoco Corporation Enhanced carotenoid accumulation in storage organs of genetically engineered plants
US5837848A (en) 1990-03-16 1998-11-17 Zeneca Limited Root-specific promoter
IL101508A0 (en) 1991-04-08 1992-12-30 Rhone Poulenc Agrochimie Chimeric plant genes based on upstream regulatory elements of helianthinin
US8030546B2 (en) 1998-09-22 2011-10-04 Mendel Biotechnology, Inc. Biotic and abiotic stress tolerance in plants
US20050086718A1 (en) 1999-03-23 2005-04-21 Mendel Biotechnology, Inc. Plant transcriptional regulators of abiotic stress
EP1613730A4 (en) 2003-03-28 2007-12-05 Monsanto Technology Llc NEW PLANT PROMOTORS FOR USE IN THE EARLY SEED DEVELOPMENT PHASE
JP2005304409A (ja) 2004-04-23 2005-11-04 Yamasa Shoyu Co Ltd 表皮に傷を付けた丸大豆及びその利用
CL2007001851A1 (es) 2006-06-23 2008-01-18 Monsanto Technology Llc Socedad Anonima Organizada Bajo Las Leyes Del Estado De Delaware Segmento de adn de planta que comprende una secuencia recombinante de un factor de transcripcion nuclear y (nf-yb), celula vegetal que lo comprende y metodo para elaborar semillas que comprende seleccionar plantas que comprendan dicho adn.
EP2471355A1 (en) 2006-08-07 2012-07-04 Mendel Biotechnology, Inc. Plants with enhanced size and growth rate
EP1917858A1 (en) 2006-09-29 2008-05-07 Syngeta Participations AG A method for enhancing plant tolerance
JP5735775B2 (ja) * 2009-10-21 2015-06-17 花王株式会社 改変プロモーター
US9157091B2 (en) 2011-02-24 2015-10-13 Iowa State University Research Foundation, Inc. Materials and method for modifying a biochemical component in a plant
US10415053B2 (en) * 2011-02-24 2019-09-17 Iowa State University Research Foundation, Inc. Materials and method for increasing a plant's resistance to stress
CN103509094B (zh) * 2012-06-25 2015-09-02 中国农业科学院作物科学研究所 植物耐逆性相关蛋白GmNF-YC9及其编码基因与应用
US20140186957A1 (en) * 2013-01-03 2014-07-03 Fred Hutchinson Cancer Research Center Engineered tal effector proteins with enhanced dna targeting capacity
KR101380049B1 (ko) * 2013-02-12 2014-04-01 경상대학교산학협력단 B―box 도메인 단백질들 및 이를 이용한 식물의 개화시기 조절방법
IL289396B2 (en) * 2013-03-15 2023-12-01 The General Hospital Coporation Using truncated guide rnas (tru-grnas) to increase specificity for rna-guided genome editing
AU2016220088C1 (en) 2015-02-18 2022-12-08 Iowa State University Research Foundation, Inc. Modification of transcriptional repressor binding site in NF-YC4 promoter for increased protein content and resistance to stress
CN112795572B (zh) * 2020-11-13 2022-12-27 中国农业大学 月季核因子RhNF-YC9及其在调控花瓣扩展中的应用
US20230151378A1 (en) * 2021-10-28 2023-05-18 Board Of Trustees Of Southern Illinois University Soybean Lines with High Seed Protein and Steady to High Oil Content

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