CO2019007828A2 - Evento de élite ee-gm5 y métodos y kits para identificar dicho evento en muestras biológicas - Google Patents
Evento de élite ee-gm5 y métodos y kits para identificar dicho evento en muestras biológicasInfo
- Publication number
- CO2019007828A2 CO2019007828A2 CONC2019/0007828A CO2019007828A CO2019007828A2 CO 2019007828 A2 CO2019007828 A2 CO 2019007828A2 CO 2019007828 A CO2019007828 A CO 2019007828A CO 2019007828 A2 CO2019007828 A2 CO 2019007828A2
- Authority
- CO
- Colombia
- Prior art keywords
- event
- biological samples
- kits
- identify
- methods
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
RESUMEN La invención proporciona plantas, material de planta y semillas de soja transgénicas específicas, caracterizadas porque estos productos albergan un evento de transformación de resistencia a nematodo y tolerancia a herbicida específico en una localización específica en el genoma de soja. También se proporcionan herramientas que permiten la identificación rápida e inequívoca del evento en muestras biológicas.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662437874P | 2016-12-22 | 2016-12-22 | |
US201762481292P | 2017-04-04 | 2017-04-04 | |
PCT/US2017/068121 WO2018119364A1 (en) | 2016-12-22 | 2017-12-22 | Elite event ee-gm5 and methods and kits for identifying such event in biological samples |
Publications (1)
Publication Number | Publication Date |
---|---|
CO2019007828A2 true CO2019007828A2 (es) | 2019-10-09 |
Family
ID=62627466
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CONC2019/0007828A CO2019007828A2 (es) | 2016-12-22 | 2019-07-22 | Evento de élite ee-gm5 y métodos y kits para identificar dicho evento en muestras biológicas |
Country Status (15)
Country | Link |
---|---|
US (2) | US11555202B2 (es) |
EP (1) | EP3559242A1 (es) |
JP (2) | JP2020513753A (es) |
KR (1) | KR20190097191A (es) |
CN (1) | CN110382703B (es) |
AU (2) | AU2017382333B2 (es) |
BR (1) | BR112019012499A2 (es) |
CA (1) | CA3046098A1 (es) |
CL (1) | CL2019001742A1 (es) |
CO (1) | CO2019007828A2 (es) |
IL (1) | IL267429A (es) |
MX (1) | MX2019007593A (es) |
PH (1) | PH12019501412A1 (es) |
UY (1) | UY37549A (es) |
WO (1) | WO2018119364A1 (es) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR112019012947A2 (pt) * | 2016-12-22 | 2019-11-26 | BASF Agricultural Solutions Seed US LLC | moléculas de ácido nucleico, dna, plantas, produto de soja, métodos para produção de um produto de soja, para proteção de plantas, para produção de uma planta, para identificação, pares de iniciadores, para confirmação da pureza de sementes, para determinação do estado de zigosidade, para redução da perda de rendimento e para aumento do rendimento de plantas, processo para controle de ervas daninhas, uso, sondas, estojo e método de detecção da presença do evento de elite |
US20210198685A1 (en) * | 2018-05-25 | 2021-07-01 | BASF Agricultural Solutions Seed US LLC | Plants containing elite event ee-gm4 and methods and kits for identifying such event in biological samples, and treatment thereof |
CN112673095A (zh) * | 2018-05-25 | 2021-04-16 | 巴斯夫农业种子解决方案美国有限责任公司 | 含有优良事件ee-gm5的植物,和用于在生物样品中识别所述事件的方法和试剂盒,以及其处理 |
CN110923356B (zh) * | 2019-12-30 | 2022-08-12 | 广西大学 | 一种水稻抗稻瘿蚊主效基因Gm5的分子标记引物及其标记方法和应用 |
GB202214203D0 (en) | 2022-09-28 | 2022-11-09 | Syngenta Crop Protection Ag | Fungicidal compositions |
GB202214202D0 (en) | 2022-09-28 | 2022-11-09 | Syngenta Crop Protection Ag | Agricultural methods |
WO2024100069A1 (en) | 2022-11-08 | 2024-05-16 | Syngenta Crop Protection Ag | Microbiocidal pyridine derivatives |
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US5846717A (en) | 1996-01-24 | 1998-12-08 | Third Wave Technologies, Inc. | Detection of nucleic acid sequences by invader-directed cleavage |
US5985557A (en) | 1996-01-24 | 1999-11-16 | Third Wave Technologies, Inc. | Invasive cleavage of nucleic acids |
US6245968B1 (en) * | 1997-11-07 | 2001-06-12 | Aventis Cropscience S.A. | Mutated hydroxyphenylpyruvate dioxygenase, DNA sequence and isolation of plants which contain such a gene and which are tolerant to herbicides |
US20080168583A1 (en) | 1999-08-19 | 2008-07-10 | Fincher Karen L | Nucleic acid molecules and other molecules associated with plants |
JP5256020B2 (ja) | 2005-04-08 | 2013-08-07 | バイエル・クロップサイエンス・エヌ・ヴェー | エリートイベントa2704−12、ならびに生物サンプル中の該イベントを同定するための方法およびキット |
MX2007012561A (es) | 2005-04-11 | 2008-02-22 | Bayer Bioscience Nv | Evento elite a5547-127, y metodos y equipos para identificar dicho evento en muestras biologicas. |
PT1885176T (pt) | 2005-05-27 | 2016-11-28 | Monsanto Technology Llc | Evento mon89788 de soja e métodos para a sua deteção |
GB0510979D0 (en) | 2005-05-28 | 2005-07-06 | Kbiosciences Ltd | Detection system for PCR assay |
AU2007260716B2 (en) | 2006-06-14 | 2013-05-02 | BASF Agricultural Solutions Seed US LLC | Axmi-031, axmi-039, axmi-040 and axmi-049, a family of delta-endotoxin genes and methods for their use |
US7951995B2 (en) | 2006-06-28 | 2011-05-31 | Pioneer Hi-Bred International, Inc. | Soybean event 3560.4.3.5 and compositions and methods for the identification and detection thereof |
EP2078089B1 (en) | 2006-10-31 | 2016-05-04 | E. I. du Pont de Nemours and Company | Soybean event dp-305423-1 and compositions and methods for the identification and/or detection thereof |
BRPI0820373B1 (pt) | 2007-11-15 | 2024-01-02 | Monsanto Technology Llc | Método de produção de uma planta de soja resistente a inseto, composições derivadas de células de tal planta, método para proteção de uma planta de soja de infestação de inseto, moléculas de dna, métodos de detectar a presença de tais moléculas e de determinar a zigozidade de tais plantas e kit de detecção de dna |
JP5767813B2 (ja) | 2008-02-15 | 2015-08-19 | モンサント テクノロジー エルエルシー | トランスジェニック事象mon87769に対応するダイズ植物および種子ならびにそれらを検出する方法 |
BRPI0918840A2 (pt) | 2008-08-25 | 2015-08-18 | Dow Agrosciences Llc | Vetor de expressão de planta, proteína modificada, método de produção da referida proteína e método para inibição de um nematoide |
BRPI0917140A2 (pt) | 2008-08-25 | 2015-07-28 | Dow Agrosciences Llc | Proteínas cry6 modificadas de bacillus thuringiensis para controle de nematoides |
US20110231963A1 (en) | 2008-08-25 | 2011-09-22 | Dow Agrosciences Llc | Modified bacillus thuringiensis cry14 proteins for nematode control |
US20120110706A1 (en) | 2008-08-25 | 2012-05-03 | Dow Agrosciences Llc | Modified bacillus thuringiensis cry21 proteins for nematode control |
BRPI0917185A2 (pt) | 2008-08-25 | 2015-07-28 | Dow Agrosciences Llc | Proteínas cry12 do bacillus thuringiensis modificadas para o controle de nematódeo |
US9078406B2 (en) | 2008-08-29 | 2015-07-14 | Monsanto Technology Llc | Soybean plant and seed corresponding to transgenic event MON87754 and methods for detection thereof |
AR075549A1 (es) | 2008-09-29 | 2011-04-20 | Monsanto Technology Llc | Evento transgenico de soja mon87705 y metodos para detectar el mismo |
MX2011005778A (es) * | 2008-12-03 | 2011-07-20 | Basf Plant Science Gmbh | Plantas que tienen tolerancia al estres abiotico y/o mejores rasgos relacionados con el rendimiento y un metodo para producirlas. |
WO2010080829A1 (en) | 2009-01-07 | 2010-07-15 | Basf Agrochemical Products B.V. | Soybean event 127 and methods related thereto |
JP5726878B2 (ja) | 2009-09-17 | 2015-06-03 | モンサント テクノロジー エルエルシー | ダイズ・トランスジェニック事象mon87708およびその使用方法 |
EP2503873B1 (en) | 2009-11-23 | 2017-02-08 | Bayer CropScience NV | Herbicide tolerant soybean plants and methods for identifying same |
US9631202B2 (en) | 2009-11-23 | 2017-04-25 | M.S. Technologies Llc | Elite event EE-GM3 and methods and kits for identifying such event in biological samples |
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JP5805767B2 (ja) | 2010-09-01 | 2015-11-10 | バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングBayer Intellectual Property GmbH | N−(テトラゾール−5−イル)アリールカルボキサミド類及びn−(トリアゾール−5−イル)アリールカルボキサミド類並びに除草剤としてのそれらの使用 |
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2017
- 2017-12-22 WO PCT/US2017/068121 patent/WO2018119364A1/en unknown
- 2017-12-22 MX MX2019007593A patent/MX2019007593A/es unknown
- 2017-12-22 AU AU2017382333A patent/AU2017382333B2/en active Active
- 2017-12-22 JP JP2019533363A patent/JP2020513753A/ja active Pending
- 2017-12-22 EP EP17832658.3A patent/EP3559242A1/en active Pending
- 2017-12-22 KR KR1020197020827A patent/KR20190097191A/ko not_active Application Discontinuation
- 2017-12-22 UY UY0001037549A patent/UY37549A/es unknown
- 2017-12-22 BR BR112019012499A patent/BR112019012499A2/pt unknown
- 2017-12-22 US US16/471,776 patent/US11555202B2/en active Active
- 2017-12-22 CN CN201780087352.7A patent/CN110382703B/zh active Active
- 2017-12-22 CA CA3046098A patent/CA3046098A1/en active Pending
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2019
- 2019-06-17 IL IL267429A patent/IL267429A/en unknown
- 2019-06-19 PH PH12019501412A patent/PH12019501412A1/en unknown
- 2019-06-20 CL CL2019001742A patent/CL2019001742A1/es unknown
- 2019-07-22 CO CONC2019/0007828A patent/CO2019007828A2/es unknown
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2022
- 2022-11-23 US US18/058,537 patent/US20230151381A1/en active Pending
- 2022-12-27 JP JP2022209195A patent/JP2023052098A/ja active Pending
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2024
- 2024-05-14 AU AU2024203184A patent/AU2024203184A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
US20230151381A1 (en) | 2023-05-18 |
PH12019501412A1 (en) | 2020-06-08 |
US11555202B2 (en) | 2023-01-17 |
WO2018119364A1 (en) | 2018-06-28 |
BR112019012499A2 (pt) | 2020-04-14 |
MX2019007593A (es) | 2019-12-02 |
UY37549A (es) | 2018-06-29 |
AU2017382333B2 (en) | 2024-02-15 |
CA3046098A1 (en) | 2018-06-28 |
JP2020513753A (ja) | 2020-05-21 |
CN110382703B (zh) | 2024-04-16 |
EP3559242A1 (en) | 2019-10-30 |
AU2017382333A1 (en) | 2019-06-20 |
CN110382703A (zh) | 2019-10-25 |
JP2023052098A (ja) | 2023-04-11 |
IL267429A (en) | 2019-08-29 |
KR20190097191A (ko) | 2019-08-20 |
AU2024203184A1 (en) | 2024-06-06 |
US20200123561A1 (en) | 2020-04-23 |
CL2019001742A1 (es) | 2019-10-04 |
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