SG10202010311SA - Novel Crispr Enzymes and Systems - Google Patents
Novel Crispr Enzymes and SystemsInfo
- Publication number
- SG10202010311SA SG10202010311SA SG10202010311SA SG10202010311SA SG10202010311SA SG 10202010311S A SG10202010311S A SG 10202010311SA SG 10202010311S A SG10202010311S A SG 10202010311SA SG 10202010311S A SG10202010311S A SG 10202010311SA SG 10202010311S A SG10202010311S A SG 10202010311SA
- Authority
- SG
- Singapore
- Prior art keywords
- systems
- crispr enzymes
- novel crispr
- novel
- enzymes
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662324820P | 2016-04-19 | 2016-04-19 | |
US201662351558P | 2016-06-17 | 2016-06-17 | |
US201662360765P | 2016-07-11 | 2016-07-11 | |
US201662410196P | 2016-10-19 | 2016-10-19 |
Publications (1)
Publication Number | Publication Date |
---|---|
SG10202010311SA true SG10202010311SA (en) | 2020-11-27 |
Family
ID=58739338
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG10202010311SA SG10202010311SA (en) | 2016-04-19 | 2017-04-19 | Novel Crispr Enzymes and Systems |
SG11201810179RA SG11201810179RA (en) | 2016-04-19 | 2017-04-19 | Novel crispr enzymes and systems |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201810179RA SG11201810179RA (en) | 2016-04-19 | 2017-04-19 | Novel crispr enzymes and systems |
Country Status (8)
Country | Link |
---|---|
US (1) | US20210155911A1 (en) |
EP (1) | EP3445848A1 (en) |
KR (2) | KR102424476B1 (en) |
CN (1) | CN110382692A (en) |
AU (2) | AU2017253089B2 (en) |
CA (1) | CA3026055A1 (en) |
SG (2) | SG10202010311SA (en) |
WO (1) | WO2017184768A1 (en) |
Families Citing this family (100)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2853829C (en) | 2011-07-22 | 2023-09-26 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
US10704021B2 (en) | 2012-03-15 | 2020-07-07 | Flodesign Sonics, Inc. | Acoustic perfusion devices |
US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
WO2015105955A1 (en) | 2014-01-08 | 2015-07-16 | Flodesign Sonics, Inc. | Acoustophoresis device with dual acoustophoretic chamber |
WO2015134812A1 (en) | 2014-03-05 | 2015-09-11 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for treating usher syndrome and retinitis pigmentosa |
US11141493B2 (en) | 2014-03-10 | 2021-10-12 | Editas Medicine, Inc. | Compositions and methods for treating CEP290-associated disease |
EP3116997B1 (en) | 2014-03-10 | 2019-05-15 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for treating leber's congenital amaurosis 10 (lca10) |
US11339437B2 (en) | 2014-03-10 | 2022-05-24 | Editas Medicine, Inc. | Compositions and methods for treating CEP290-associated disease |
US11242525B2 (en) | 2014-03-26 | 2022-02-08 | Editas Medicine, Inc. | CRISPR/CAS-related methods and compositions for treating sickle cell disease |
WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
EP3215617A2 (en) | 2014-11-07 | 2017-09-13 | Editas Medicine, Inc. | Methods for improving crispr/cas-mediated genome-editing |
GB201506509D0 (en) | 2015-04-16 | 2015-06-03 | Univ Wageningen | Nuclease-mediated genome editing |
KR102535217B1 (en) | 2015-04-24 | 2023-05-19 | 에디타스 메디신, 인코포레이티드 | Assessment of CAS9 Molecule/Guide RNA Molecule Complexes |
US11708572B2 (en) | 2015-04-29 | 2023-07-25 | Flodesign Sonics, Inc. | Acoustic cell separation techniques and processes |
US11377651B2 (en) | 2016-10-19 | 2022-07-05 | Flodesign Sonics, Inc. | Cell therapy processes utilizing acoustophoresis |
WO2016182959A1 (en) | 2015-05-11 | 2016-11-17 | Editas Medicine, Inc. | Optimized crispr/cas9 systems and methods for gene editing in stem cells |
WO2016201047A1 (en) | 2015-06-09 | 2016-12-15 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for improving transplantation |
US10648020B2 (en) | 2015-06-18 | 2020-05-12 | The Broad Institute, Inc. | CRISPR enzymes and systems |
CA2999500A1 (en) | 2015-09-24 | 2017-03-30 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
US10167457B2 (en) | 2015-10-23 | 2019-01-01 | President And Fellows Of Harvard College | Nucleobase editors and uses thereof |
WO2017165826A1 (en) | 2016-03-25 | 2017-09-28 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
US11236313B2 (en) | 2016-04-13 | 2022-02-01 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
US11214789B2 (en) | 2016-05-03 | 2022-01-04 | Flodesign Sonics, Inc. | Concentration and washing of particles with acoustics |
BR112019001887A2 (en) | 2016-08-02 | 2019-07-09 | Editas Medicine Inc | compositions and methods for treating cep290-associated disease |
IL308426A (en) | 2016-08-03 | 2024-01-01 | Harvard College | Adenosine nucleobase editors and uses thereof |
CN109804066A (en) | 2016-08-09 | 2019-05-24 | 哈佛大学的校长及成员们 | Programmable CAS9- recombination enzyme fusion proteins and application thereof |
WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
AU2017342543A1 (en) | 2016-10-14 | 2019-05-02 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
WO2018098383A1 (en) * | 2016-11-22 | 2018-05-31 | Integrated Dna Technologies, Inc. | Crispr/cpf1 systems and methods |
WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
EP3596217A1 (en) | 2017-03-14 | 2020-01-22 | Editas Medicine, Inc. | Systems and methods for the treatment of hemoglobinopathies |
JP7191388B2 (en) | 2017-03-23 | 2022-12-19 | プレジデント アンド フェローズ オブ ハーバード カレッジ | Nucleobase editors comprising nucleic acid programmable DNA binding proteins |
US11591589B2 (en) | 2017-04-21 | 2023-02-28 | The General Hospital Corporation | Variants of Cpf1 (Cas12a) with altered PAM specificity |
WO2018201086A1 (en) | 2017-04-28 | 2018-11-01 | Editas Medicine, Inc. | Methods and systems for analyzing guide rna molecules |
EP3622070A2 (en) | 2017-05-10 | 2020-03-18 | Editas Medicine, Inc. | Crispr/rna-guided nuclease systems and methods |
US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
EP3635113A4 (en) | 2017-06-05 | 2021-03-17 | Fred Hutchinson Cancer Research Center | Genomic safe harbors for genetic therapies in human stem cells and engineered nanoparticles to provide targeted genetic therapies |
AU2018279829B2 (en) | 2017-06-09 | 2024-01-04 | Editas Medicine, Inc. | Engineered Cas9 nucleases |
US11866726B2 (en) | 2017-07-14 | 2024-01-09 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
EP3658573A1 (en) | 2017-07-28 | 2020-06-03 | President and Fellows of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace) |
JP2020532967A (en) | 2017-08-23 | 2020-11-19 | ザ ジェネラル ホスピタル コーポレイション | Genetically engineered CRISPR-Cas9 nuclease with altered PAM specificity |
US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
WO2019041296A1 (en) * | 2017-09-01 | 2019-03-07 | 上海科技大学 | Base editing system and method |
EP3697906A1 (en) | 2017-10-16 | 2020-08-26 | The Broad Institute, Inc. | Uses of adenosine base editors |
US10253365B1 (en) | 2017-11-22 | 2019-04-09 | The Regents Of The University Of California | Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs |
EP3724326A1 (en) * | 2017-12-11 | 2020-10-21 | Editas Medicine, Inc. | Cpf1-related methods and compositions for gene editing |
CA3085784A1 (en) | 2017-12-14 | 2019-06-20 | Flodesign Sonics, Inc. | Acoustic transducer driver and controller |
CA3086550A1 (en) * | 2017-12-22 | 2019-06-27 | The Broad Institute, Inc. | Crispr effector system based multiplex diagnostics |
CN109957569B (en) * | 2017-12-22 | 2022-10-25 | 苏州齐禾生科生物科技有限公司 | Base editing system and method based on CPF1 protein |
WO2019138052A1 (en) * | 2018-01-11 | 2019-07-18 | Kws Saat Se | Optimized plant crispr/cpf1 systems |
CN112020558A (en) | 2018-03-14 | 2020-12-01 | 爱迪塔斯医药公司 | Systems and methods for treating hemoglobinopathies |
CN108486146B (en) * | 2018-03-16 | 2021-02-19 | 中国农业科学院作物科学研究所 | Application of LbCpf1-RR mutant in CRISPR/Cpf1 system in plant gene editing |
EP3790963A4 (en) * | 2018-05-11 | 2022-04-20 | Beam Therapeutics, Inc. | Methods of editing single nucleotide polymorphism using programmable base editor systems |
RU2703532C1 (en) * | 2018-06-15 | 2019-10-21 | ФБУН Центральный НИИ эпидемиологии Роспотребнадзора | System for activating human apobec/aid cytidine deaminases and/or human uracil-dna-glycosylase ung and its use for eliminating ccc dna of hepatitis b virus from human cells, particularly hepatocytes |
JP2021528091A (en) | 2018-06-26 | 2021-10-21 | ザ・ブロード・インスティテュート・インコーポレイテッド | Compositions, Systems, and Methods for Amplification Based on CRISPR Double Nickase |
CN112543812A (en) | 2018-06-26 | 2021-03-23 | 麻省理工学院 | Amplification methods, systems and diagnostics based on CRISPR effector systems |
WO2020028729A1 (en) | 2018-08-01 | 2020-02-06 | Mammoth Biosciences, Inc. | Programmable nuclease compositions and methods of use thereof |
EP3861012A4 (en) * | 2018-10-04 | 2022-10-19 | The Regents of the University of Colorado, a Body Corporate | Engineered chimeric nucleic acid guided nucleases, compositions, methods for making, and systems for gene editing |
EP3861112A4 (en) * | 2018-10-04 | 2022-09-21 | The Regents of the University of Colorado, A Body Corporate | Engineered chimeric nucleic acid guided nuclease constructs and uses thereof |
WO2020124050A1 (en) | 2018-12-13 | 2020-06-18 | The Broad Institute, Inc. | Tiled assays using crispr-cas based detection |
WO2020142730A1 (en) * | 2019-01-04 | 2020-07-09 | Stellate Biotherapeutics, Inc. | Targeted genome modification using circular single-stranded dna |
EP3931313A2 (en) | 2019-01-04 | 2022-01-05 | Mammoth Biosciences, Inc. | Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection |
EP3927820A4 (en) * | 2019-02-22 | 2024-03-27 | Integrated Dna Tech Inc | Lachnospiraceae bacterium nd2006 cas12a mutant genes and polypeptides encoded by same |
US20220154258A1 (en) | 2019-03-14 | 2022-05-19 | The Broad Institute, Inc. | Crispr effector system based multiplex diagnostics |
DE112020001342T5 (en) | 2019-03-19 | 2022-01-13 | President and Fellows of Harvard College | Methods and compositions for editing nucleotide sequences |
WO2020236645A1 (en) * | 2019-05-17 | 2020-11-26 | Beth Israel Deaconess Medical Center, Inc. | Compositions and methods for homology directed repair |
JP2022539248A (en) | 2019-07-02 | 2022-09-07 | フレッド ハッチンソン キャンサー リサーチ センター | Recombinant AD35 vectors and related gene therapy improvements |
US20220333208A1 (en) | 2019-09-03 | 2022-10-20 | The Broad Institute, Inc. | Crispr effector system based multiplex cancer diagnostics |
EP4045651A1 (en) * | 2019-10-14 | 2022-08-24 | KWS SAAT SE & Co. KGaA | Mad7 nuclease in plants and expanding its pam recognition capability |
KR20220097408A (en) | 2019-10-17 | 2022-07-07 | 페어와이즈 플랜츠 서비시즈, 인크. | Variants of CAS12A nucleases and methods for their preparation and uses |
WO2021081384A1 (en) * | 2019-10-25 | 2021-04-29 | Greenvenus, Llc | Synthetic nucleases |
US11844800B2 (en) | 2019-10-30 | 2023-12-19 | Massachusetts Institute Of Technology | Methods and compositions for predicting and preventing relapse of acute lymphoblastic leukemia |
CN110747187B (en) * | 2019-11-13 | 2022-10-21 | 电子科技大学 | Cas12a protein for identifying TTTV and TTV double-PAM sites, plant genome directed editing vector and method |
EP4069829A1 (en) | 2019-12-08 | 2022-10-12 | Editas Medicine, Inc. | Modified cells and methods for the treatment of hemoglobinopathies |
JP2023525304A (en) | 2020-05-08 | 2023-06-15 | ザ ブロード インスティテュート,インコーポレーテッド | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
CN111904973B (en) * | 2020-07-27 | 2021-12-03 | 中国农业科学院兰州兽医研究所 | Application of ssc-miR-122 in preparation of medicine for regulating replication of porcine reproductive and respiratory syndrome virus |
WO2022051418A1 (en) | 2020-09-01 | 2022-03-10 | 64-X, Inc. | Mammalian cells and methods for engineering the same |
CN112111470B (en) * | 2020-09-10 | 2022-06-14 | 武汉大学 | R-ring binding protein GST-His6-1/2 XHBD and method for detecting whole genome R-loop |
CN112111471B (en) * | 2020-09-25 | 2022-05-03 | 中国科学院微生物研究所 | FnCpf1 mutant for identifying PAM sequence in broad spectrum and application thereof |
CN116334037A (en) * | 2020-11-11 | 2023-06-27 | 山东舜丰生物科技有限公司 | Novel Cas enzymes and systems and uses |
CN113308451B (en) * | 2020-12-07 | 2023-07-25 | 中国科学院动物研究所 | Engineered Cas effector proteins and methods of use thereof |
CN115335536A (en) * | 2021-01-08 | 2022-11-11 | 武汉大学 | Compositions and methods for point-of-care nucleic acid detection |
JP2024506608A (en) * | 2021-02-08 | 2024-02-14 | エメンドバイオ・インコーポレイテッド | OMNI-103 CRISPR nuclease |
CA3216359A1 (en) * | 2021-04-07 | 2022-10-13 | Battelle Memorial Institute | Rapid design, build, test, and learn technologies for identifying and using non-viral carriers |
WO2022217086A1 (en) | 2021-04-09 | 2022-10-13 | Vor Biopharma Inc. | Photocleavable guide rnas and methods of use thereof |
IL308806A (en) | 2021-06-01 | 2024-01-01 | Arbor Biotechnologies Inc | Gene editing systems comprising a crispr nuclease and uses thereof |
WO2023283585A2 (en) | 2021-07-06 | 2023-01-12 | Vor Biopharma Inc. | Inhibitor oligonucleotides and methods of use thereof |
AU2022324093A1 (en) | 2021-08-02 | 2024-02-08 | Vor Biopharma Inc. | Compositions and methods for gene modification |
AU2022325054A1 (en) | 2021-08-02 | 2024-02-01 | Editas Medicine, Inc. | Systems and methods for the treatment of hemoglobinopathies |
KR102356172B1 (en) * | 2021-08-24 | 2022-02-08 | (주)코미코 | Method for Producing Plasma-Resistant Coating Layer |
WO2023049926A2 (en) | 2021-09-27 | 2023-03-30 | Vor Biopharma Inc. | Fusion polypeptides for genetic editing and methods of use thereof |
WO2024073751A1 (en) | 2022-09-29 | 2024-04-04 | Vor Biopharma Inc. | Methods and compositions for gene modification and enrichment |
CN116286734B (en) * | 2022-11-29 | 2024-04-02 | 武汉大学 | Mutant of wild LbCAs12a protein and SNP detection application |
Family Cites Families (115)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4217344A (en) | 1976-06-23 | 1980-08-12 | L'oreal | Compositions containing aqueous dispersions of lipid spheres |
US4235871A (en) | 1978-02-24 | 1980-11-25 | Papahadjopoulos Demetrios P | Method of encapsulating biologically active materials in lipid vesicles |
US4186183A (en) | 1978-03-29 | 1980-01-29 | The United States Of America As Represented By The Secretary Of The Army | Liposome carriers in chemotherapy of leishmaniasis |
US4261975A (en) | 1979-09-19 | 1981-04-14 | Merck & Co., Inc. | Viral liposome particle |
US4485054A (en) | 1982-10-04 | 1984-11-27 | Lipoderm Pharmaceuticals Limited | Method of encapsulating biologically active materials in multilamellar lipid vesicles (MLV) |
US4501728A (en) | 1983-01-06 | 1985-02-26 | Technology Unlimited, Inc. | Masking of liposomes from RES recognition |
US4897355A (en) | 1985-01-07 | 1990-01-30 | Syntex (U.S.A.) Inc. | N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
US4946787A (en) | 1985-01-07 | 1990-08-07 | Syntex (U.S.A.) Inc. | N-(ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
US5049386A (en) | 1985-01-07 | 1991-09-17 | Syntex (U.S.A.) Inc. | N-ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)Alk-1-YL-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
US4797368A (en) | 1985-03-15 | 1989-01-10 | The United States Of America As Represented By The Department Of Health And Human Services | Adeno-associated virus as eukaryotic expression vector |
US4774085A (en) | 1985-07-09 | 1988-09-27 | 501 Board of Regents, Univ. of Texas | Pharmaceutical administration systems containing a mixture of immunomodulators |
ATE141646T1 (en) | 1986-04-09 | 1996-09-15 | Genzyme Corp | GENETICALLY TRANSFORMED ANIMALS THAT SECRETE A DESIRED PROTEIN IN MILK |
US4837028A (en) | 1986-12-24 | 1989-06-06 | Liposome Technology, Inc. | Liposomes with enhanced circulation time |
US4873316A (en) | 1987-06-23 | 1989-10-10 | Biogen, Inc. | Isolation of exogenous recombinant proteins from the milk of transgenic mammals |
US5703055A (en) | 1989-03-21 | 1997-12-30 | Wisconsin Alumni Research Foundation | Generation of antibodies through lipid mediated DNA delivery |
US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
US5264618A (en) | 1990-04-19 | 1993-11-23 | Vical, Inc. | Cationic lipids for intracellular delivery of biologically active molecules |
AU7979491A (en) | 1990-05-03 | 1991-11-27 | Vical, Inc. | Intracellular delivery of biologically active substances by means of self-assembling lipid complexes |
US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
US5173414A (en) | 1990-10-30 | 1992-12-22 | Applied Immune Sciences, Inc. | Production of recombinant adeno-associated virus vectors |
US5587308A (en) | 1992-06-02 | 1996-12-24 | The United States Of America As Represented By The Department Of Health & Human Services | Modified adeno-associated virus vector capable of expression from a novel promoter |
US5593972A (en) | 1993-01-26 | 1997-01-14 | The Wistar Institute | Genetic immunization |
US5543158A (en) | 1993-07-23 | 1996-08-06 | Massachusetts Institute Of Technology | Biodegradable injectable nanoparticles |
US6007845A (en) | 1994-07-22 | 1999-12-28 | Massachusetts Institute Of Technology | Nanoparticles and microparticles of non-linear hydrophilic-hydrophobic multiblock copolymers |
AU698739B2 (en) | 1995-06-06 | 1998-11-05 | Isis Pharmaceuticals, Inc. | Oligonucleotides having phosphorothioate linkages of high chiral purity |
US5985662A (en) | 1995-07-13 | 1999-11-16 | Isis Pharmaceuticals Inc. | Antisense inhibition of hepatitis B virus replication |
US5855913A (en) | 1997-01-16 | 1999-01-05 | Massachusetts Instite Of Technology | Particles incorporating surfactants for pulmonary drug delivery |
US5985309A (en) | 1996-05-24 | 1999-11-16 | Massachusetts Institute Of Technology | Preparation of particles for inhalation |
US5846946A (en) | 1996-06-14 | 1998-12-08 | Pasteur Merieux Serums Et Vaccins | Compositions and methods for administering Borrelia DNA |
US5944710A (en) | 1996-06-24 | 1999-08-31 | Genetronics, Inc. | Electroporation-mediated intravascular delivery |
US5869326A (en) | 1996-09-09 | 1999-02-09 | Genetronics, Inc. | Electroporation employing user-configured pulsing scheme |
GB9907461D0 (en) | 1999-03-31 | 1999-05-26 | King S College London | Neurite regeneration |
GB9710049D0 (en) | 1997-05-19 | 1997-07-09 | Nycomed Imaging As | Method |
GB9720465D0 (en) | 1997-09-25 | 1997-11-26 | Oxford Biomedica Ltd | Dual-virus vectors |
DE69836092T2 (en) | 1997-10-24 | 2007-05-10 | Invitrogen Corp., Carlsbad | RECOMBINATORY CLONING USING NUCLEAR FACILITIES WITH RECOMBINATION CENTERS |
US6750059B1 (en) | 1998-07-16 | 2004-06-15 | Whatman, Inc. | Archiving of vectors |
US6534261B1 (en) | 1999-01-12 | 2003-03-18 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
US7868149B2 (en) | 1999-07-20 | 2011-01-11 | Monsanto Technology Llc | Plant genome sequence and uses thereof |
US6603061B1 (en) | 1999-07-29 | 2003-08-05 | Monsanto Company | Agrobacterium-mediated plant transformation method |
GB0024550D0 (en) | 2000-10-06 | 2000-11-22 | Oxford Biomedica Ltd | |
US20020150626A1 (en) | 2000-10-16 | 2002-10-17 | Kohane Daniel S. | Lipid-protein-sugar particles for delivery of nucleic acids |
US7776321B2 (en) | 2001-09-26 | 2010-08-17 | Mayo Foundation For Medical Education And Research | Mutable vaccines |
GB0125216D0 (en) | 2001-10-19 | 2001-12-12 | Univ Strathclyde | Dendrimers for use in targeted delivery |
US20090100536A1 (en) | 2001-12-04 | 2009-04-16 | Monsanto Company | Transgenic plants with enhanced agronomic traits |
AU2002353231B2 (en) | 2001-12-21 | 2008-10-16 | Oxford Biomedica (Uk) Limited | Method for producing a transgenic organism using a lentiviral expression vector such as EIAV |
EP2338478B1 (en) | 2002-06-28 | 2014-07-23 | Protiva Biotherapeutics Inc. | Method for producing liposomes |
GB0220467D0 (en) | 2002-09-03 | 2002-10-09 | Oxford Biomedica Ltd | Composition |
WO2005007196A2 (en) | 2003-07-16 | 2005-01-27 | Protiva Biotherapeutics, Inc. | Lipid encapsulated interfering rna |
NZ581166A (en) | 2003-09-15 | 2011-06-30 | Protiva Biotherapeutics Inc | Polyethyleneglycol-modified lipid compounds and uses thereof |
US20050123596A1 (en) | 2003-09-23 | 2005-06-09 | Kohane Daniel S. | pH-triggered microparticles |
GB0325379D0 (en) | 2003-10-30 | 2003-12-03 | Oxford Biomedica Ltd | Vectors |
CA2569645C (en) | 2004-06-07 | 2014-10-28 | Protiva Biotherapeutics, Inc. | Cationic lipids and methods of use |
WO2005121348A1 (en) | 2004-06-07 | 2005-12-22 | Protiva Biotherapeutics, Inc. | Lipid encapsulated interfering rna |
EP1784416B1 (en) | 2004-07-16 | 2011-10-05 | GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES | Vaccines against aids comprising cmv/r nucleic acid constructs |
GB0422877D0 (en) | 2004-10-14 | 2004-11-17 | Univ Glasgow | Bioactive polymers |
WO2008036075A2 (en) | 2005-08-10 | 2008-03-27 | Northwestern University | Composite particles |
WO2007048046A2 (en) | 2005-10-20 | 2007-04-26 | Protiva Biotherapeutics, Inc. | Sirna silencing of filovirus gene expression |
ES2377660T3 (en) | 2005-10-28 | 2012-03-29 | Mitsubishi Tanabe Pharma Corporation | New peptide that penetrates cells |
EP2395012B8 (en) | 2005-11-02 | 2018-06-06 | Arbutus Biopharma Corporation | Modified siRNA molecules and uses thereof |
GB0526211D0 (en) | 2005-12-22 | 2006-02-01 | Oxford Biomedica Ltd | Viral vectors |
US7915399B2 (en) | 2006-06-09 | 2011-03-29 | Protiva Biotherapeutics, Inc. | Modified siRNA molecules and uses thereof |
JP2008078613A (en) | 2006-08-24 | 2008-04-03 | Rohm Co Ltd | Method of producing nitride semiconductor, and nitride semiconductor element |
DK2121977T3 (en) | 2007-01-11 | 2017-09-18 | Erasmus Univ Medical Center | Capture (4C) OF CHROMOSOMES WITH CIRCULAR CONFORMATION |
WO2009023798A2 (en) | 2007-08-14 | 2009-02-19 | Fred Hutchinson Cancer Research Center | Needle array assembly and method for delivering therapeutic agents |
CA2708153C (en) | 2007-12-04 | 2017-09-26 | Alnylam Pharmaceuticals, Inc. | Carbohydrate conjugates as delivery agents for oligonucleotides |
AU2008346801A1 (en) | 2007-12-31 | 2009-07-16 | Nanocor Therapeutics, Inc. | RNA interference for the treatment of heart failure |
US9688718B2 (en) | 2008-01-11 | 2017-06-27 | Lawrence Livermore National Security, Llc | Nanolipoprotein particles comprising hydrogenases and related products, methods and systems |
PL2279254T3 (en) | 2008-04-15 | 2017-11-30 | Protiva Biotherapeutics Inc. | Novel lipid formulations for nucleic acid delivery |
US8575305B2 (en) | 2008-06-04 | 2013-11-05 | Medical Research Council | Cell penetrating peptides |
WO2010001325A2 (en) | 2008-06-30 | 2010-01-07 | Silenseed Ltd | Methods, compositions and systems for local delivery of drugs |
EP2309980A1 (en) | 2008-07-08 | 2011-04-20 | S.I.F.I. Societa' Industria Farmaceutica Italiana | Ophthalmic compositions for treating pathologies of the posterior segment of the eye |
US20110212179A1 (en) | 2008-10-30 | 2011-09-01 | David Liu | Micro-spherical porous biocompatible scaffolds and methods and apparatus for fabricating same |
AU2009311667B2 (en) | 2008-11-07 | 2016-04-14 | Massachusetts Institute Of Technology | Aminoalcohol lipidoids and uses thereof |
WO2010078569A2 (en) | 2009-01-05 | 2010-07-08 | Stc.Unm | Porous nanoparticle supported lipid bilayer nanostructures |
EP2427577A4 (en) | 2009-05-04 | 2013-10-23 | Hutchinson Fred Cancer Res | Cocal vesiculovirus envelope pseudotyped retroviral vectors |
EP2449114B9 (en) | 2009-07-01 | 2017-04-19 | Protiva Biotherapeutics Inc. | Novel lipid formulations for delivery of therapeutic agents to solid tumors |
US8236943B2 (en) | 2009-07-01 | 2012-08-07 | Protiva Biotherapeutics, Inc. | Compositions and methods for silencing apolipoprotein B |
WO2011028929A2 (en) | 2009-09-03 | 2011-03-10 | The Regents Of The University Of California | Nitrate-responsive promoter |
US8889394B2 (en) | 2009-09-07 | 2014-11-18 | Empire Technology Development Llc | Multiple domain proteins |
EP3023495B1 (en) | 2010-02-24 | 2019-05-08 | Arrowhead Pharmaceuticals, Inc. | Compositions for targeted delivery of sirna |
US8372951B2 (en) | 2010-05-14 | 2013-02-12 | National Tsing Hua University | Cell penetrating peptides for intracellular delivery |
EP2575894B1 (en) | 2010-05-28 | 2015-02-25 | Oxford Biomedica (UK) Ltd | Delivery of lentiviral vectors to the brain |
WO2012027675A2 (en) | 2010-08-26 | 2012-03-01 | Massachusetts Institute Of Technology | Poly(beta-amino alcohols), their preparation, and uses thereof |
US20120190609A1 (en) | 2010-08-30 | 2012-07-26 | Martin Bader | Method for producing a lipid particle, the lipid particle itself and its use |
US9405700B2 (en) | 2010-11-04 | 2016-08-02 | Sonics, Inc. | Methods and apparatus for virtualization in an integrated circuit |
DK2691443T3 (en) | 2011-03-28 | 2021-05-03 | Massachusetts Inst Technology | CONJUGIATED LIPOMERS AND USES OF THESE |
CA2831613A1 (en) | 2011-03-31 | 2012-10-04 | Moderna Therapeutics, Inc. | Delivery and formulation of engineered nucleic acids |
US20120295960A1 (en) | 2011-05-20 | 2012-11-22 | Oxford Biomedica (Uk) Ltd. | Treatment regimen for parkinson's disease |
EP3915545A1 (en) | 2011-10-25 | 2021-12-01 | The University of British Columbia | Limit size lipid nanoparticles and related methods |
US20140308304A1 (en) | 2011-12-07 | 2014-10-16 | Alnylam Pharmaceuticals, Inc. | Lipids for the delivery of active agents |
EP2791160B1 (en) | 2011-12-16 | 2022-03-02 | ModernaTX, Inc. | Modified mrna compositions |
WO2013158141A1 (en) | 2012-04-18 | 2013-10-24 | Arrowhead Research Corporation | Poly(acrylate) polymers for in vivo nucleic acid delivery |
JP2015527889A (en) | 2012-07-25 | 2015-09-24 | ザ ブロード インスティテュート, インコーポレイテッド | Inducible DNA binding protein and genomic disruption tools and their applications |
US8614194B1 (en) | 2012-07-25 | 2013-12-24 | Kaohsiung Medical University | Anionic cell penetrating peptide and its use for intracellular delivery |
ES2883590T3 (en) | 2012-12-12 | 2021-12-09 | Broad Inst Inc | Supply, modification and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications |
SG10201801969TA (en) | 2012-12-12 | 2018-04-27 | Broad Inst Inc | Engineering and Optimization of Improved Systems, Methods and Enzyme Compositions for Sequence Manipulation |
EP2931899A1 (en) | 2012-12-12 | 2015-10-21 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
US20140242664A1 (en) * | 2012-12-12 | 2014-08-28 | The Broad Institute, Inc. | Engineering of systems, methods and optimized guide compositions for sequence manipulation |
SG11201504621RA (en) | 2012-12-17 | 2015-07-30 | Harvard College | Rna-guided human genome engineering |
WO2014118272A1 (en) | 2013-01-30 | 2014-08-07 | Santaris Pharma A/S | Antimir-122 oligonucleotide carbohydrate conjugates |
US9693958B2 (en) | 2013-03-15 | 2017-07-04 | Cureport, Inc. | Methods and devices for preparation of lipid nanoparticles |
WO2014186366A1 (en) | 2013-05-13 | 2014-11-20 | Tufts University | Nanocomplexes for delivery of saporin |
WO2014186348A2 (en) | 2013-05-14 | 2014-11-20 | Tufts University | Nanocomplexes of modified peptides or proteins |
US9267135B2 (en) | 2013-06-04 | 2016-02-23 | President And Fellows Of Harvard College | RNA-guided transcriptional regulation |
US10563225B2 (en) | 2013-07-26 | 2020-02-18 | President And Fellows Of Harvard College | Genome engineering |
EP3066201B1 (en) | 2013-11-07 | 2018-03-07 | Editas Medicine, Inc. | Crispr-related methods and compositions with governing grnas |
BR112016013213A2 (en) | 2013-12-12 | 2017-12-05 | Massachusetts Inst Technology | administration, use and therapeutic applications of crisper systems and compositions for targeting disorders and diseases using particle delivery components |
CA2932472A1 (en) | 2013-12-12 | 2015-06-18 | Massachusetts Institute Of Technology | Compositions and methods of use of crispr-cas systems in nucleotide repeat disorders |
WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
US11172675B2 (en) | 2014-12-22 | 2021-11-16 | Oro Agri Inc. | Nano particulate delivery system |
GB201506509D0 (en) * | 2015-04-16 | 2015-06-03 | Univ Wageningen | Nuclease-mediated genome editing |
US9790490B2 (en) * | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
RS64331B1 (en) | 2015-06-19 | 2023-08-31 | Massachusetts Inst Technology | Alkenyl substituted 2,5-piperazinediones and their use in compositions for delivering an agent to a subject or cell |
CN108601823A (en) | 2015-09-23 | 2018-09-28 | 麻省理工学院 | Composition for being modified dendrimer nanoparticles vaccine delivery and method |
US20190264186A1 (en) * | 2016-01-22 | 2019-08-29 | The Broad Institute Inc. | Crystal structure of crispr cpf1 |
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