EP3861012A4 - Engineered chimeric nucleic acid guided nucleases, compositions, methods for making, and systems for gene editing - Google Patents

Engineered chimeric nucleic acid guided nucleases, compositions, methods for making, and systems for gene editing Download PDF

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Publication number
EP3861012A4
EP3861012A4 EP19868458.1A EP19868458A EP3861012A4 EP 3861012 A4 EP3861012 A4 EP 3861012A4 EP 19868458 A EP19868458 A EP 19868458A EP 3861012 A4 EP3861012 A4 EP 3861012A4
Authority
EP
European Patent Office
Prior art keywords
compositions
systems
making
methods
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19868458.1A
Other languages
German (de)
French (fr)
Other versions
EP3861012A1 (en
Inventor
Ryan T. Gill
Rongming LIU
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Colorado
Original Assignee
University of Colorado
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Colorado filed Critical University of Colorado
Publication of EP3861012A1 publication Critical patent/EP3861012A1/en
Publication of EP3861012A4 publication Critical patent/EP3861012A4/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
EP19868458.1A 2018-10-04 2019-10-04 Engineered chimeric nucleic acid guided nucleases, compositions, methods for making, and systems for gene editing Withdrawn EP3861012A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862741475P 2018-10-04 2018-10-04
US201862741470P 2018-10-04 2018-10-04
PCT/US2019/054869 WO2020073005A1 (en) 2018-10-04 2019-10-04 Engineered chimeric nucleic acid guided nucleases, compositions, methods for making, and systems for gene editing

Publications (2)

Publication Number Publication Date
EP3861012A1 EP3861012A1 (en) 2021-08-11
EP3861012A4 true EP3861012A4 (en) 2022-10-19

Family

ID=70054911

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19868458.1A Withdrawn EP3861012A4 (en) 2018-10-04 2019-10-04 Engineered chimeric nucleic acid guided nucleases, compositions, methods for making, and systems for gene editing

Country Status (2)

Country Link
EP (1) EP3861012A4 (en)
WO (1) WO2020073005A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021151065A2 (en) * 2020-01-24 2021-07-29 The General Hospital Corporation Methods to characterize enzymes for genome engineering

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017184768A1 (en) * 2016-04-19 2017-10-26 The Broad Institute Inc. Novel crispr enzymes and systems
WO2018071672A1 (en) * 2016-10-12 2018-04-19 The Regents Of The University Of Colorado Novel engineered and chimeric nucleases

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017184768A1 (en) * 2016-04-19 2017-10-26 The Broad Institute Inc. Novel crispr enzymes and systems
WO2018071672A1 (en) * 2016-10-12 2018-04-19 The Regents Of The University Of Colorado Novel engineered and chimeric nucleases

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DAAN C. SWARTS ET AL: "Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a", MOLECULAR CELL, vol. 66, no. 2, 1 April 2017 (2017-04-01), AMSTERDAM, NL, pages 221 - 233, XP055569665, ISSN: 1097-2765, DOI: 10.1016/j.molcel.2017.03.016 *
DATABASE Geneseq [online] 31 May 2018 (2018-05-31), "Eubacterium rectale derived CpF1 nuclease, SEQ:2.", XP055925363, retrieved from EBI accession no. GSP:BFF09218 Database accession no. BFF09218 *
LINYI GAO ET AL: "Engineered Cpf1 variants with altered PAM specificities", NATURE BIOTECHNOLOGY, 5 June 2017 (2017-06-05), New York, XP055396069, ISSN: 1087-0156, DOI: 10.1038/nbt.3900 *
LIU R. M. ET AL: "Abstract", NATURE COMMUNICATIONS, vol. 10, no. 1, 1 December 2019 (2019-12-01), XP055785454, DOI: 10.1038/s41467-019-13500-y *
LUTZ S ET AL: "Creating multiple-crossover DNA libraries independent of sequence identity", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 98, no. 20, 25 September 2001 (2001-09-25), pages 11248 - 11253, XP002250112, ISSN: 0027-8424, DOI: 10.1073/PNAS.201413698 *
See also references of WO2020073005A1 *

Also Published As

Publication number Publication date
WO2020073005A1 (en) 2020-04-09
EP3861012A1 (en) 2021-08-11

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