CN115052980A - 衍生自黄杆菌的基因编辑系统 - Google Patents

衍生自黄杆菌的基因编辑系统 Download PDF

Info

Publication number
CN115052980A
CN115052980A CN202080080579.0A CN202080080579A CN115052980A CN 115052980 A CN115052980 A CN 115052980A CN 202080080579 A CN202080080579 A CN 202080080579A CN 115052980 A CN115052980 A CN 115052980A
Authority
CN
China
Prior art keywords
guide rna
cas12a protein
sequence
seq
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202080080579.0A
Other languages
English (en)
Inventor
高彩霞
靳帅
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Suzhou Qihe Biotechnology Co ltd
Original Assignee
Shanghai Blue Cross Medical Science Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai Blue Cross Medical Science Research Institute filed Critical Shanghai Blue Cross Medical Science Research Institute
Publication of CN115052980A publication Critical patent/CN115052980A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

本发明属于基因工程领域。具体而言，本发明公开了一种衍生自黄杆菌的基因编辑系统及其应用。

Description

PCT国内申请，说明书已公开。

Claims (16)

  1. PCT国内申请，权利要求书已公开。
CN202080080579.0A 2019-11-18 2020-11-18 衍生自黄杆菌的基因编辑系统 Pending CN115052980A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2019111263484 2019-11-18
CN201911126348 2019-11-18
PCT/CN2020/129665 WO2021098709A1 (zh) 2019-11-18 2020-11-18 衍生自黄杆菌的基因编辑系统

Publications (1)

Publication Number Publication Date
CN115052980A true CN115052980A (zh) 2022-09-13

Family

ID=75981339

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080080579.0A Pending CN115052980A (zh) 2019-11-18 2020-11-18 衍生自黄杆菌的基因编辑系统

Country Status (5)

Country Link
US (1) US20230002453A1 (zh)
EP (1) EP4063500A4 (zh)
CN (1) CN115052980A (zh)
BR (1) BR112022009584A2 (zh)
WO (1) WO2021098709A1 (zh)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108513582A (zh) * 2015-06-18 2018-09-07 布罗德研究所有限公司 新型crispr酶以及系统
CN109312316A (zh) * 2016-02-15 2019-02-05 本森希尔生物系统股份有限公司 修饰基因组的组合物和方法
CN109688820A (zh) * 2016-06-24 2019-04-26 科罗拉多州立大学董事会（法人团体） 用于生成条形码化组合文库的方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10513711B2 (en) * 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
EA038321B1 (ru) * 2014-11-06 2021-08-09 Е.И. Дюпон Де Немур Энд Компани Опосредуемая пептидом доставка направляемой рнк эндонуклеазы в клетки
US10648020B2 (en) * 2015-06-18 2020-05-12 The Broad Institute, Inc. CRISPR enzymes and systems
WO2017189308A1 (en) * 2016-04-19 2017-11-02 The Broad Institute Inc. Novel crispr enzymes and systems
US20190330659A1 (en) * 2016-07-15 2019-10-31 Zymergen Inc. Scarless dna assembly and genome editing using crispr/cpf1 and dna ligase
KR20190116282A (ko) * 2017-02-10 2019-10-14 지머젠 인코포레이티드 복수의 숙주를 위한 다중 dna 구조체의 조립 및 편집을 위한 모듈식 범용 플라스미드 디자인 전략
MA52134A (fr) * 2017-06-09 2020-04-15 Vilmorin & Cie Compositions et procédés pour l'édition génomique
CN111225975A (zh) * 2017-09-11 2020-06-02 加利福尼亚大学董事会 抗体介导的cas9向哺乳动物细胞的递送
EP3790969A1 (en) * 2018-05-09 2021-03-17 DSM IP Assets B.V. Crispr transient expression construct (ctec)
CN110117621B (zh) * 2019-05-24 2021-07-16 青岛农业大学 一种碱基编辑器及其制备方法和应用

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108513582A (zh) * 2015-06-18 2018-09-07 布罗德研究所有限公司 新型crispr酶以及系统
CN109312316A (zh) * 2016-02-15 2019-02-05 本森希尔生物系统股份有限公司 修饰基因组的组合物和方法
CN109688820A (zh) * 2016-06-24 2019-04-26 科罗拉多州立大学董事会（法人团体） 用于生成条形码化组合文库的方法

Also Published As

Publication number Publication date
WO2021098709A1 (zh) 2021-05-27
EP4063500A1 (en) 2022-09-28
US20230002453A1 (en) 2023-01-05
BR112022009584A2 (pt) 2022-10-04
EP4063500A4 (en) 2023-12-27

Similar Documents

Publication Publication Date Title
WO2019120310A1 (en) Base editing system and method based on cpf1 protein
WO2019120283A1 (en) Method for base editing in plants
CN108690845B (zh) 基因组编辑系统和方法
CN109689875B (zh) 基因组编辑系统和方法
WO2020224611A1 (en) Improved gene editing system
CN114945670A (zh) 一种碱基编辑系统和其使用方法
US20240117368A1 (en) Multiplex genome editing method and system
CN117187220A (zh) 腺嘌呤脱氨酶及其在碱基编辑中的用途
CN114945671A (zh) 靶向性修饰植物基因组序列的方法
CN110527695A (zh) 一种用于基因定点突变的核酸构建物
CN112805385B (zh) 基于人apobec3a脱氨酶的碱基编辑器及其用途
CN112280771A (zh) 双功能基因组编辑系统及其用途
JP7361109B2 (ja) Ｃ２ｃ１ヌクレアーゼに基づくゲノム編集のためのシステムおよび方法
US20230313234A1 (en) Improved cytosine base editing system
WO2023030534A1 (zh) 改进的引导编辑系统
CN101560251B (zh) 植物根生长发育相关蛋白及其编码基因与应用
CN115052980A (zh) 衍生自黄杆菌的基因编辑系统
WO2022188816A1 (zh) 改进的cg碱基编辑系统
CN108841861A (zh) 蛋白TaNADH-GoGAT调控植物根系发育的应用
WO2024051850A1 (zh) 基于dna聚合酶的基因组编辑系统和方法
WO2022057780A1 (zh) 一个调控植物氮素利用效率和产量的蛋白质及其应用
EP4317430A1 (en) Method for improving plant genetic transformation and gene editing efficiency
CN117187213A (zh) 新的crispr基因编辑系统
CN113025597A (zh) 改进的基因组编辑系统
CN117126876A (zh) 一种在基因组中定点插入外源序列的方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right

Effective date of registration: 20220928

Address after: Unit E598, 5th Floor, Lecheng Plaza, Phase II, Biomedical Industrial Park, No. 218, Sangtian Street, Suzhou Industrial Park, Suzhou Area, China (Jiangsu) Pilot Free Trade Zone, Suzhou City, Jiangsu Province, 215127

Applicant after: Suzhou Qihe Biotechnology Co.,Ltd.

Address before: Room D340, F3, building 2, No. 2250, Pudong South Road, Pudong New Area, Shanghai 200120

Applicant before: Shanghai Blue Cross Medical Science Research Institute

TA01 Transfer of patent application right