CN114945671A - 靶向性修饰植物基因组序列的方法 - Google Patents

靶向性修饰植物基因组序列的方法 Download PDF

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CN114945671A
CN114945671A CN202080077133.2A CN202080077133A CN114945671A CN 114945671 A CN114945671 A CN 114945671A CN 202080077133 A CN202080077133 A CN 202080077133A CN 114945671 A CN114945671 A CN 114945671A
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sequence
pegrna
plant
target
genome
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高彩霞
林秋鹏
宗媛
靳帅
薛郴销
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Suzhou Qihe Biotechnology Co ltd
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Shanghai Blue Cross Medical Science Research Institute
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

提供了一种通过向导RNA指导的核酸酶‑逆转录酶融合蛋白将植物基因组中的特定序列靶向性修改为感兴趣的目的序列的方法，以及通过该方法产生的经遗传修饰的植物及其后代。

Description

PCT国内申请，说明书已公开。

Claims (19)

  1. PCT国内申请，权利要求书已公开。
CN202080077133.2A 2019-11-01 2020-09-25 靶向性修饰植物基因组序列的方法 Pending CN114945671A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
CN2019110620056 2019-11-01
CN201911062005 2019-11-01
CN202010036374 2020-01-14
CN2020100363744 2020-01-14
PCT/CN2020/117736 WO2021082830A1 (zh) 2019-11-01 2020-09-25 靶向性修饰植物基因组序列的方法

Publications (1)

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CN114945671A true CN114945671A (zh) 2022-08-26

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US (1) US20230075587A1 (zh)
EP (1) EP4053284A4 (zh)
CN (1) CN114945671A (zh)
BR (1) BR112022008468A2 (zh)
WO (1) WO2021082830A1 (zh)

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Publication number Priority date Publication date Assignee Title
WO2022242660A1 (en) * 2021-05-17 2022-11-24 Wuhan University System and methods for insertion and editing of large nucleic acid fragments
CN116555224A (zh) * 2021-09-06 2023-08-08 苏州齐禾生科生物科技有限公司 改进的引导编辑系统
US11866728B2 (en) 2022-01-21 2024-01-09 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
CN117126876A (zh) * 2022-05-25 2023-11-28 中国科学院遗传与发育生物学研究所 一种在基因组中定点插入外源序列的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014186686A2 (en) * 2013-05-17 2014-11-20 Two Blades Foundation Targeted mutagenesis and genome engineering in plants using rna-guided cas nucleases
CN107109422A (zh) * 2014-11-19 2017-08-29 基础科学研究院 使用由两个载体表达的Cas9蛋白质调节基因表达的方法
CN108546716A (zh) * 2017-02-20 2018-09-18 中国科学院遗传与发育生物学研究所 一种基因组编辑方法

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Publication number Priority date Publication date Assignee Title
MX2021011426A (es) * 2019-03-19 2022-03-11 Broad Inst Inc Metodos y composiciones para editar secuencias de nucleótidos.
WO2021072328A1 (en) * 2019-10-10 2021-04-15 The Broad Institute, Inc. Methods and compositions for prime editing rna
AU2020369599A1 (en) * 2019-10-23 2022-05-12 Monsanto Technology Llc Compositions and methods for RNA-templated editing in plants
CN111378051B (zh) * 2020-03-25 2022-03-01 北京市农林科学院 Pe-p2引导编辑系统及其在基因组碱基编辑中的应用

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014186686A2 (en) * 2013-05-17 2014-11-20 Two Blades Foundation Targeted mutagenesis and genome engineering in plants using rna-guided cas nucleases
CN107109422A (zh) * 2014-11-19 2017-08-29 基础科学研究院 使用由两个载体表达的Cas9蛋白质调节基因表达的方法
CN108546716A (zh) * 2017-02-20 2018-09-18 中国科学院遗传与发育生物学研究所 一种基因组编辑方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ANDREW V. ANZALONE等: "Search-and-replace genome editing without double-strand breaks or donor DNA", NATURE, vol. 576, no. 7785, pages 1 - 2 *
聂丽娜;夏兰琴;徐兆师;高东尧;李琳;于卓;陈明;李连城;马有志;: "植物基因启动子的克隆及其功能研究进展", 植物遗传资源学报, no. 03 *

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US20230075587A1 (en) 2023-03-09
EP4053284A4 (en) 2024-03-06
BR112022008468A2 (pt) 2022-07-19
WO2021082830A1 (zh) 2021-05-06
EP4053284A1 (en) 2022-09-07

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