BR112022008468A2 - Método para modificação direcionada de uma sequência de genoma de planta - Google Patents

Método para modificação direcionada de uma sequência de genoma de planta

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Publication number
BR112022008468A2
BR112022008468A2 BR112022008468A BR112022008468A BR112022008468A2 BR 112022008468 A2 BR112022008468 A2 BR 112022008468A2 BR 112022008468 A BR112022008468 A BR 112022008468A BR 112022008468 A BR112022008468 A BR 112022008468A BR 112022008468 A2 BR112022008468 A2 BR 112022008468A2
Authority
BR
Brazil
Prior art keywords
targeted modification
plant genome
plant
genome sequence
relates
Prior art date
Application number
BR112022008468A
Other languages
English (en)
Inventor
Gao Caixia
Lin Qiupeng
Zong Yuan
Jin Shuai
Xue Chenxiao
Original Assignee
Shanghai Bluecross Medical Science Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai Bluecross Medical Science Inst filed Critical Shanghai Bluecross Medical Science Inst
Publication of BR112022008468A2 publication Critical patent/BR112022008468A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

MÉTODO PARA MODIFICAÇÃO DIRECIONADA DE UMA SEQUÊNCIA DE GENOMA DE PLANTA. A presente invenção refere-se ao campo da engenharia genética de plantas. Especificamente, a presente invenção refere-se a um método para modificação direcionada de uma sequência do genoma vegetal. Mais especificamente, a presente invenção refere-se a um método para modificação direcionada de uma sequência específica no genoma de planta em uma sequência alvo de interesse por uma proteína de fusão nuclease-transcriptase reversa guiada por um RNA guia, uma planta geneticamente modificada produzida pelo referido método e progênies da referida planta.
BR112022008468A 2019-11-01 2020-09-25 Método para modificação direcionada de uma sequência de genoma de planta BR112022008468A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201911062005 2019-11-01
CN202010036374 2020-01-14
PCT/CN2020/117736 WO2021082830A1 (zh) 2019-11-01 2020-09-25 靶向性修饰植物基因组序列的方法

Publications (1)

Publication Number Publication Date
BR112022008468A2 true BR112022008468A2 (pt) 2022-07-19

Family

ID=75715770

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022008468A BR112022008468A2 (pt) 2019-11-01 2020-09-25 Método para modificação direcionada de uma sequência de genoma de planta

Country Status (5)

Country Link
US (1) US20230075587A1 (pt)
EP (1) EP4053284A4 (pt)
CN (1) CN114945671A (pt)
BR (1) BR112022008468A2 (pt)
WO (1) WO2021082830A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118043457A (zh) * 2021-05-17 2024-05-14 武汉大学 用于插入和编辑大核酸片段的系统和方法
KR20240049838A (ko) * 2021-09-06 2024-04-17 쑤저우 치 바이오디자인 바이오테크놀로지 컴퍼니 리미티드 개선된 가이드된 에디팅 시스템
US11866728B2 (en) 2022-01-21 2024-01-09 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2023227050A1 (zh) * 2022-05-25 2023-11-30 中国科学院遗传与发育生物学研究所 一种在基因组中定点插入外源序列的方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014186686A2 (en) * 2013-05-17 2014-11-20 Two Blades Foundation Targeted mutagenesis and genome engineering in plants using rna-guided cas nucleases
WO2016080795A1 (ko) * 2014-11-19 2016-05-26 기초과학연구원 두 개의 벡터로부터 발현된 cas9 단백질을 이용한 유전자 발현 조절 방법
US20200224221A1 (en) 2017-02-20 2020-07-16 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Genome editing method
EP3942040A1 (en) * 2019-03-19 2022-01-26 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
WO2021072328A1 (en) * 2019-10-10 2021-04-15 The Broad Institute, Inc. Methods and compositions for prime editing rna
CN115279898A (zh) * 2019-10-23 2022-11-01 成对植物服务股份有限公司 用于植物中rna模板化编辑的组合物和方法
CN111378051B (zh) * 2020-03-25 2022-03-01 北京市农林科学院 Pe-p2引导编辑系统及其在基因组碱基编辑中的应用

Also Published As

Publication number Publication date
EP4053284A1 (en) 2022-09-07
CN114945671A (zh) 2022-08-26
WO2021082830A1 (zh) 2021-05-06
US20230075587A1 (en) 2023-03-09
EP4053284A4 (en) 2024-03-06

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