JP3917731B2 - レーザ走査顕微鏡 - Google Patents
レーザ走査顕微鏡 Download PDFInfo
- Publication number
- JP3917731B2 JP3917731B2 JP29410797A JP29410797A JP3917731B2 JP 3917731 B2 JP3917731 B2 JP 3917731B2 JP 29410797 A JP29410797 A JP 29410797A JP 29410797 A JP29410797 A JP 29410797A JP 3917731 B2 JP3917731 B2 JP 3917731B2
- Authority
- JP
- Japan
- Prior art keywords
- coherent light
- laser
- light
- scanning
- specimen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000003287 optical effect Effects 0.000 claims description 236
- 230000001427 coherent effect Effects 0.000 claims description 140
- 238000006243 chemical reaction Methods 0.000 claims description 42
- 238000012545 processing Methods 0.000 claims description 21
- 238000001514 detection method Methods 0.000 claims description 19
- 238000010521 absorption reaction Methods 0.000 claims description 13
- 230000001678 irradiating effect Effects 0.000 claims description 12
- 238000012790 confirmation Methods 0.000 claims description 9
- 230000008878 coupling Effects 0.000 claims 5
- 238000010168 coupling process Methods 0.000 claims 5
- 238000005859 coupling reaction Methods 0.000 claims 5
- 238000003384 imaging method Methods 0.000 description 44
- 210000001747 pupil Anatomy 0.000 description 25
- 238000010586 diagram Methods 0.000 description 24
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 8
- OZLGRUXZXMRXGP-UHFFFAOYSA-N Fluo-3 Chemical compound CC1=CC=C(N(CC(O)=O)CC(O)=O)C(OCCOC=2C(=CC=C(C=2)C2=C3C=C(Cl)C(=O)C=C3OC3=CC(O)=C(Cl)C=C32)N(CC(O)=O)CC(O)=O)=C1 OZLGRUXZXMRXGP-UHFFFAOYSA-N 0.000 description 8
- 239000003269 fluorescent indicator Substances 0.000 description 8
- 238000002834 transmittance Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 230000005284 excitation Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 4
- 229910052786 argon Inorganic materials 0.000 description 4
- 229910001424 calcium ion Inorganic materials 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 210000002889 endothelial cell Anatomy 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 210000005036 nerve Anatomy 0.000 description 3
- 210000000329 smooth muscle myocyte Anatomy 0.000 description 3
- 238000003325 tomography Methods 0.000 description 3
- 210000001367 artery Anatomy 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000002073 fluorescence micrograph Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29410797A JP3917731B2 (ja) | 1996-11-21 | 1997-10-27 | レーザ走査顕微鏡 |
| US08/972,591 US6094300A (en) | 1996-11-21 | 1997-11-18 | Laser scanning microscope |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8-310889 | 1996-11-21 | ||
| JP31088996 | 1996-11-21 | ||
| JP29410797A JP3917731B2 (ja) | 1996-11-21 | 1997-10-27 | レーザ走査顕微鏡 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JPH10206742A JPH10206742A (ja) | 1998-08-07 |
| JPH10206742A5 JPH10206742A5 (enExample) | 2005-10-27 |
| JP3917731B2 true JP3917731B2 (ja) | 2007-05-23 |
Family
ID=26559679
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP29410797A Expired - Lifetime JP3917731B2 (ja) | 1996-11-21 | 1997-10-27 | レーザ走査顕微鏡 |
Country Status (2)
| Country | Link |
|---|---|
| US (1) | US6094300A (enExample) |
| JP (1) | JP3917731B2 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10281699B2 (en) | 2016-08-29 | 2019-05-07 | Olympus Corporation | Microscope system configured to irradiate focused light onto an area of a specimen outside of an optical axis of an objective lens |
Families Citing this family (117)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19531988A1 (de) * | 1995-08-30 | 1997-03-06 | Europaeische Kommission | Fernmessung von U (Pu) in Gläsern |
| JP3411780B2 (ja) * | 1997-04-07 | 2003-06-03 | レーザーテック株式会社 | レーザ顕微鏡及びこのレーザ顕微鏡を用いたパターン検査装置 |
| US6383816B1 (en) * | 1998-11-10 | 2002-05-07 | University Of Delaware | Probing of surface roughness |
| JP3283499B2 (ja) * | 1999-03-18 | 2002-05-20 | オリンパス光学工業株式会社 | レーザ顕微鏡 |
| DE19930532C2 (de) * | 1999-06-30 | 2002-03-28 | Zeiss Carl Jena Gmbh | Anordnung zur Optimierung der Pulsform in einem Laser-Scanning-Mikroskop |
| JP4512219B2 (ja) * | 1999-09-21 | 2010-07-28 | オリンパス株式会社 | 光走査型顕微鏡 |
| DE19954933A1 (de) * | 1999-11-10 | 2001-05-17 | Zeiss Carl Jena Gmbh | Anordnung zur Einkopplung einer optischen Pinzette und/oder eines Bearbeitungsstrahles in ein Mikroskop |
| JP4671463B2 (ja) * | 2000-03-24 | 2011-04-20 | オリンパス株式会社 | 照明光学系及び照明光学系を備えた顕微鏡 |
| DE10039520A1 (de) * | 2000-08-08 | 2002-02-21 | Leica Microsystems | Vorrichtung zur Untersuchung und Manipulation von mikroskopischen Objekten |
| JP4653292B2 (ja) * | 2000-08-09 | 2011-03-16 | オリンパス株式会社 | システム顕微鏡 |
| US7383076B2 (en) | 2000-11-27 | 2008-06-03 | The General Hospital Corporation | Fluorescence-mediated molecular tomography |
| US6615063B1 (en) * | 2000-11-27 | 2003-09-02 | The General Hospital Corporation | Fluorescence-mediated molecular tomography |
| US6643049B2 (en) * | 2001-02-01 | 2003-11-04 | Kodak Polychrome Graphics Llc | Compact imaging head and high speed multi-head laser imaging assembly and method |
| DE10105391B4 (de) * | 2001-02-06 | 2004-11-25 | Leica Microsystems Heidelberg Gmbh | Scanmikroskop und Modul für ein Scanmikroskop |
| JP4804665B2 (ja) * | 2001-08-09 | 2011-11-02 | オリンパス株式会社 | レーザ顕微鏡 |
| US7196843B2 (en) | 2002-03-27 | 2007-03-27 | Olympus Optical Co., Ltd. | Confocal microscope apparatus |
| JP4632634B2 (ja) * | 2002-03-27 | 2011-02-16 | オリンパス株式会社 | 共焦点顕微鏡装置及び共焦点顕微鏡装置を用いた観察方法 |
| JP4242617B2 (ja) * | 2002-08-28 | 2009-03-25 | オリンパス株式会社 | 走査型レーザ顕微鏡システム |
| JP2004110017A (ja) * | 2002-08-29 | 2004-04-08 | Olympus Corp | 走査型レーザ顕微鏡 |
| US7223986B2 (en) * | 2002-08-29 | 2007-05-29 | Olympus Optical Co., Ltd. | Laser scanning microscope |
| AU2002347689A1 (en) * | 2002-09-27 | 2004-04-19 | Wilson O. Garcia | Two-color (two-photon) excitation with focused excitation beams and a raman shifter |
| JP4239166B2 (ja) * | 2002-12-27 | 2009-03-18 | 関西ティー・エル・オー株式会社 | 多層観察型光学顕微鏡及び多層観察ユニット |
| JP2004317741A (ja) * | 2003-04-15 | 2004-11-11 | Olympus Corp | 顕微鏡およびその光学調整方法 |
| US7881502B2 (en) * | 2003-06-30 | 2011-02-01 | Weyerhaeuser Nr Company | Method and system for three-dimensionally imaging an apical dome of a plant embryo |
| EP1668394A1 (de) * | 2003-09-25 | 2006-06-14 | Leica Microsystems Heidelberg GmbH | Mikroskop mit evaneszenter beleuchtung |
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| JP4414722B2 (ja) * | 2003-10-15 | 2010-02-10 | オリンパス株式会社 | レーザー顕微鏡 |
| US7282716B2 (en) * | 2003-11-10 | 2007-10-16 | Technology Innovations, Llc | Digital imaging assembly and methods thereof |
| US6971754B2 (en) * | 2003-11-13 | 2005-12-06 | Itt Manufacturing Enterprises, Inc. | Lens cap retention arrangement |
| DE10356826B4 (de) * | 2003-12-05 | 2021-12-02 | Leica Microsystems Cms Gmbh | Rastermikroskop |
| JP4468684B2 (ja) | 2003-12-05 | 2010-05-26 | オリンパス株式会社 | 走査型共焦点顕微鏡装置 |
| JP4573524B2 (ja) * | 2003-12-24 | 2010-11-04 | オリンパス株式会社 | 走査型レーザー顕微鏡 |
| US20050146784A1 (en) * | 2004-01-06 | 2005-07-07 | Vogt William I. | Confocal microscope having multiple independent excitation paths |
| DE102004011770B4 (de) * | 2004-03-09 | 2005-12-15 | Leica Microsystems Heidelberg Gmbh | Mikroskop |
| DE102004016253B4 (de) * | 2004-04-02 | 2006-02-23 | Leica Microsystems Cms Gmbh | Rastermikroskop und Verfahren zur rastermikroskopischen Untersuchung einer Probe |
| JP4885429B2 (ja) * | 2004-05-13 | 2012-02-29 | オリンパス株式会社 | 光刺激装置および光走査型観察装置 |
| JP4804726B2 (ja) * | 2004-06-16 | 2011-11-02 | オリンパス株式会社 | 走査型光学観察装置 |
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| DE102004034987A1 (de) * | 2004-07-16 | 2006-02-02 | Carl Zeiss Jena Gmbh | Lichtrastermikroskop und Verwendung |
| DE102004034968A1 (de) * | 2004-07-16 | 2006-02-09 | Carl Zeiss Jena Gmbh | Lichtrastermikroskop mit linienförmiger Abtastung |
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| JP2006078772A (ja) * | 2004-09-09 | 2006-03-23 | Yokogawa Electric Corp | 共焦点顕微鏡 |
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| US10394008B2 (en) | 2016-10-19 | 2019-08-27 | Cornell University | Hyperspectral multiphoton microscope for biomedical applications |
| WO2018089839A1 (en) | 2016-11-10 | 2018-05-17 | The Trustees Of Columbia University In The City Of New York | Rapid high-resolution imaging methods for large samples |
| JP7344281B2 (ja) | 2019-03-28 | 2023-09-13 | 浜松ホトニクス株式会社 | 共焦点顕微鏡ユニット及び共焦点顕微鏡 |
| DE102020006975A1 (de) * | 2020-11-11 | 2022-05-12 | Carl Zeiss Microscopy Gmbh | Laser-Scanning-Mikroskop und Verfahren zum Justieren eines Laser-Scanning-Mikroskops |
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| JPH05134186A (ja) * | 1991-11-13 | 1993-05-28 | Olympus Optical Co Ltd | 共焦点光学系 |
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| JP3326881B2 (ja) * | 1993-06-25 | 2002-09-24 | 株式会社ニコン | 走査型光学顕微鏡 |
| JPH07199079A (ja) * | 1993-12-29 | 1995-08-04 | Olympus Optical Co Ltd | 顕微測光装置 |
| JPH07333511A (ja) * | 1994-06-08 | 1995-12-22 | Nikon Corp | 顕微鏡 |
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| JP3114533B2 (ja) * | 1994-11-24 | 2000-12-04 | 住友重機械工業株式会社 | レーザ穴あけ加工装置及びレーザ穴あけ加工方法 |
| US5880880A (en) * | 1995-01-13 | 1999-03-09 | The General Hospital Corp. | Three-dimensional scanning confocal laser microscope |
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- 1997-10-27 JP JP29410797A patent/JP3917731B2/ja not_active Expired - Lifetime
- 1997-11-18 US US08/972,591 patent/US6094300A/en not_active Expired - Lifetime
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| US10281699B2 (en) | 2016-08-29 | 2019-05-07 | Olympus Corporation | Microscope system configured to irradiate focused light onto an area of a specimen outside of an optical axis of an objective lens |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH10206742A (ja) | 1998-08-07 |
| US6094300A (en) | 2000-07-25 |
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