JP4540754B2 - Ｐｃｒ中のハイブリダイゼーションのモニタリング - Google Patents

Ｐｃｒ中のハイブリダイゼーションのモニタリング Download PDF

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Publication number
JP4540754B2
JP4540754B2 JP50091698A JP50091698A JP4540754B2 JP 4540754 B2 JP4540754 B2 JP 4540754B2 JP 50091698 A JP50091698 A JP 50091698A JP 50091698 A JP50091698 A JP 50091698A JP 4540754 B2 JP4540754 B2 JP 4540754B2
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fluorescence
amplification
probe
temperature
nucleic acid
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Expired - Lifetime
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Japanese (ja)
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JP2000509608A (ja
JP2000509608A5 (Direct
Inventor
ウィットワー，カール，ティー．
リリー，カーク，エム．
ラスマッセン，ランディ，ピー．
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UNIVERSITY OF UTHA RESEARCHFOUNDATION
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UNIVERSITY OF UTHA RESEARCHFOUNDATION
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6482—Sample cells, cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/07—Centrifugal type cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Clinical Laboratory Science (AREA)
  • Optics & Photonics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Fats And Perfumes (AREA)
JP50091698A 1996-06-04 1997-06-04 Ｐｃｒ中のハイブリダイゼーションのモニタリング Expired - Lifetime JP4540754B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US65899396A 1996-06-04 1996-06-04
US08/658,993 1996-06-04
US81826797A 1997-03-17 1997-03-17
US08/818,267 1997-03-17
PCT/US1997/010008 WO1997046714A1 (en) 1996-06-04 1997-06-04 Monitoring hybridization during pcr

Related Child Applications (3)

Application Number Title Priority Date Filing Date
JP2006352635A Division JP4118932B2 (ja) 1996-06-04 2006-12-27 蛍光ドナー−アクセプター対
JP2008026583A Division JP4401416B2 (ja) 1996-06-04 2008-02-06 Ｐｃｒ中のハイブリダイゼーションのモニタリング
JP2009192560A Division JP2010046063A (ja) 1996-06-04 2009-08-21 Ｐｃｒ中のハイブリダイゼーションのモニタリング

Publications (3)

Publication Number Publication Date
JP2000509608A JP2000509608A (ja) 2000-08-02
JP2000509608A5 JP2000509608A5 (Direct) 2007-06-07
JP4540754B2 true JP4540754B2 (ja) 2010-09-08

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ID=27097737

Family Applications (4)

Application Number Title Priority Date Filing Date
JP50091698A Expired - Lifetime JP4540754B2 (ja) 1996-06-04 1997-06-04 Ｐｃｒ中のハイブリダイゼーションのモニタリング
JP2006352635A Expired - Fee Related JP4118932B2 (ja) 1996-06-04 2006-12-27 蛍光ドナー−アクセプター対
JP2008026583A Expired - Lifetime JP4401416B2 (ja) 1996-06-04 2008-02-06 Ｐｃｒ中のハイブリダイゼーションのモニタリング
JP2009192560A Pending JP2010046063A (ja) 1996-06-04 2009-08-21 Ｐｃｒ中のハイブリダイゼーションのモニタリング

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2006352635A Expired - Fee Related JP4118932B2 (ja) 1996-06-04 2006-12-27 蛍光ドナー−アクセプター対
JP2008026583A Expired - Lifetime JP4401416B2 (ja) 1996-06-04 2008-02-06 Ｐｃｒ中のハイブリダイゼーションのモニタリング
JP2009192560A Pending JP2010046063A (ja) 1996-06-04 2009-08-21 Ｐｃｒ中のハイブリダイゼーションのモニタリング

Country Status (12)

Country Link
US (9) US6174670B1 (Direct)
EP (3) EP1033411B1 (Direct)
JP (4) JP4540754B2 (Direct)
AT (3) ATE428801T1 (Direct)
AU (1) AU726501B2 (Direct)
CA (3) CA2591550C (Direct)
DE (3) DE69735313T2 (Direct)
DK (2) DK0912766T4 (Direct)
ES (2) ES2326050T5 (Direct)
NZ (2) NZ333136A (Direct)
PT (2) PT912766E (Direct)
WO (1) WO1997046714A1 (Direct)

Families Citing this family (805)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070269799A9 (en) * 1994-06-22 2007-11-22 Zhang David Y Nucleic acid amplification methods
EP1033411B1 (en) * 1996-06-04 2006-02-22 University of Utah Research Foundation Fluorescent donor-acceptor pair
AU6534898A (en) * 1997-02-14 1998-09-08 E.I. Du Pont De Nemours And Company Detection of double-stranded dna in a homogeneous solution
US20030165888A1 (en) * 2001-07-18 2003-09-04 Brown Bob D. Oligonucleotide probes and primers comprising universal bases for diagnostic purposes
US6518017B1 (en) * 1997-10-02 2003-02-11 Oasis Biosciences Incorporated Combinatorial antisense library
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
WO1999022018A2 (en) 1997-10-27 1999-05-06 Boston Probes, Inc. Methods, kits and compositions pertaining to pna molecular beacons
EP1040202B9 (en) * 1997-10-28 2009-09-23 Los Alamos National Security, LLC Dna polymorphism identity determination using flow cytometry
JP2001521765A (ja) * 1997-11-04 2001-11-13 ロシュ ダイアグノスティックス ゲーエムベーハー 特異的かつ高感度な核酸検出方法
GB9725197D0 (en) * 1997-11-29 1998-01-28 Secr Defence Detection system
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
EP1055001A1 (en) * 1998-02-05 2000-11-29 Bavarian Nordic Research Institute A/S Quantification by inhibition of amplification
GB9803382D0 (en) 1998-02-19 1998-04-15 Secr Defence Detection system
US6270973B1 (en) * 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
DE19811731A1 (de) * 1998-03-18 1999-09-23 November Ag Molekulare Medizin Verfahren zum Nachweis einer Nukleotidsequenz
DE19811729C2 (de) * 1998-03-18 2000-05-18 November Ag Molekulare Medizin Verfahren und Vorrichtung zum Nachweis einer Nukleotidsequenz
US6361942B1 (en) 1998-03-24 2002-03-26 Boston Probes, Inc. Method, kits and compositions pertaining to detection complexes
US6132968A (en) * 1998-05-13 2000-10-17 University Of Alberta Methods for quantitating low level modifications of nucleotide sequences
GB9811483D0 (en) * 1998-05-29 1998-07-29 Photonic Research Systems Limi Luminescence assay using cyclical excitation wavelength sequence
US6780617B2 (en) 2000-12-29 2004-08-24 Chen & Chen, Llc Sample processing device and method
US7799521B2 (en) * 1998-06-24 2010-09-21 Chen & Chen, Llc Thermal cycling
GB9815799D0 (en) * 1998-07-21 1998-09-16 Pharmagene Lab Limited Quantitative analysis of gene expression
GB9819418D0 (en) * 1998-09-07 1998-10-28 Secr Defence Amplification method
GB9819417D0 (en) * 1998-09-07 1998-10-28 Secr Defence Reaction method
DE19840897A1 (de) * 1998-09-08 2000-03-09 Dirk Happich Faktor V-Leiden-Test
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
GB9821989D0 (en) * 1998-10-08 1998-12-02 Hybaid Ltd Detection of nucleic acid polymorphism
DE19850593A1 (de) * 1998-11-03 2000-05-04 Biochip Technologies Gmbh Verfahren zum Nachweis von Hybridisierungsereignissen bei DNA
US6216049B1 (en) * 1998-11-20 2001-04-10 Becton, Dickinson And Company Computerized method and apparatus for analyzing nucleic acid assay readings
EP1013775A1 (en) * 1998-12-21 2000-06-28 LUTZ, Hans Quantitative polymerase chain reaction using a fluorogenic real-time detection system
GB9901072D0 (en) * 1999-01-19 1999-03-10 Imp Cancer Res Tech Methods for detecting changes to a macromolecular component of a cell
CN1348396A (zh) 1999-03-19 2002-05-08 金克克国际有限公司 用于高效筛选的多通孔测试板
US6303305B1 (en) 1999-03-30 2001-10-16 Roche Diagnostics, Gmbh Method for quantification of an analyte
CA2365984A1 (en) * 1999-04-08 2000-10-19 Oasis Biosciences, Inc. Antisense oligonucleotides comprising universal and/or degenerate bases
GB9908437D0 (en) * 1999-04-13 1999-06-09 Minton Treharne & Davies Limit Methods of marking materials
CA2644688C (en) * 1999-04-20 2012-11-13 Kankyo Engineering Co., Ltd. Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analysing data obtained by the method
US6387621B1 (en) * 1999-04-27 2002-05-14 University Of Utah Research Foundation Automated analysis of real-time nucleic acid amplification
US6331393B1 (en) 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
NZ528967A (en) * 1999-06-22 2005-03-24 Invitrogen Corp Improved primers and methods for the amplification and discrimination of nucleic acids
AU7627100A (en) * 1999-07-27 2001-02-13 University Of Utah Research Foundation Homogeneous fluorescence method for assaying structural modifications of biomolecules
US6197520B1 (en) * 1999-08-13 2001-03-06 University Of Utah Research Foundation Solution-based color compensation adjusted for temperature and electronic gains
US7192698B1 (en) * 1999-08-17 2007-03-20 Purdue Research Foundation EphA2 as a diagnostic target for metastatic cancer
US6927203B1 (en) * 1999-08-17 2005-08-09 Purdue Research Foundation Treatment of metastatic disease
US20040170966A1 (en) * 1999-11-29 2004-09-02 Furfine Eric Steven Continuous time-resolved resonance energy-transfer assay for polynucleic acid polymerases
GB9928232D0 (en) * 1999-12-01 2000-01-26 Skelton Stephen Detection system
DE10027113A1 (de) * 1999-12-23 2001-09-27 Andreas Hoeft Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
US7700279B2 (en) * 1999-12-24 2010-04-20 Quest Diagnostics Investments Incorporated Assay for bcr/abl gene rearrangement
US7250252B2 (en) * 1999-12-30 2007-07-31 David Aaron Katz Amplification based polymorphism detection
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
GB0005281D0 (en) * 2000-03-07 2000-04-26 Secr Defence Analytical method
DE10045521A1 (de) * 2000-03-31 2001-10-04 Roche Diagnostics Gmbh Nukleinsäureamplifikationen
US6691041B2 (en) 2000-03-31 2004-02-10 Roche Molecular Systems, Inc. Method for the efficiency-corrected real-time quantification of nucleic acids
DE10018465A1 (de) * 2000-04-14 2001-10-18 Aventis Res & Tech Gmbh & Co Testsystem zum Nachweis einer Spleißreaktion, sowie dessen Verwendung
US6783934B1 (en) 2000-05-01 2004-08-31 Cepheid, Inc. Methods for quantitative analysis of nucleic acid amplification reaction
EP1158449A3 (en) * 2000-05-19 2004-12-15 Becton Dickinson and Company Computerized method and apparatus for analyzing readings of nucleic acid assays
GB0016516D0 (en) * 2000-07-06 2000-08-23 Owen Mumford Ltd Improvements relating to needle protection devices
KR100823857B1 (ko) * 2000-07-07 2008-04-21 아벤티스 파마슈티칼스 인크. 트랜스포존 매개된 다중 서열분석
CA2417986C (en) 2000-08-11 2013-11-26 University Of Utah Research Foundation Single-labeled oligonucleotide probes
US6849407B2 (en) * 2000-08-31 2005-02-01 Mayo Foundation For Medical Education And Research Detection of varicella-zoster virus
US7101976B1 (en) 2000-09-12 2006-09-05 Purdue Research Foundation EphA2 monoclonal antibodies and methods of making and using same
US6558929B2 (en) * 2000-09-15 2003-05-06 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forshung E.V. PCR reaction mixture for fluorescence-based gene expression and gene mutation analyses
US6346386B1 (en) * 2000-09-29 2002-02-12 Arup Institue Method of solution-based scanning for alterations in a DNA segment using a double-stranded DNA binding dye and fluorescence melting profiles
GB0025913D0 (en) * 2000-10-23 2000-12-06 Guldberg Per Materials and methods relating to nucleic acid amplification and profiling
AU2002210700B2 (en) * 2000-10-23 2006-09-21 Cancer Research Technology Limited Nucleic acid amplification-based methods for the determination of a methylation profile and reagents therefor
US8900811B2 (en) 2000-11-16 2014-12-02 Caliper Life Sciences, Inc. Method and apparatus for generating thermal melting curves in a microfluidic device
ATE300050T1 (de) * 2000-11-16 2005-08-15 Hoffmann La Roche Farbstoffpaare für fluoreszenz-resonanz-energie- transfer (fret) messungen
US6312929B1 (en) * 2000-12-22 2001-11-06 Cepheid Compositions and methods enabling a totally internally controlled amplification reaction
US7691571B2 (en) * 2001-01-31 2010-04-06 Mayo Foundation For Medical Education And Research Detection of bordetella
ATE465272T1 (de) 2001-01-31 2010-05-15 Mayo Foundation Nachweis von herpex-simplex-virus
WO2002061141A1 (en) * 2001-01-31 2002-08-08 Mayo Foundation For Medical Education And Research Detection of bordetella
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
US7559879B2 (en) * 2001-04-16 2009-07-14 Brunswick Corporation Stride adjustment mechanism
DE60228952D1 (de) 2001-05-07 2008-10-30 Mayo Foundation Nachweis von Legionella durch ein PCR und FRET verwendendes Verfahren
US6979530B2 (en) 2001-05-21 2005-12-27 Applera Corporation Peptide conjugates and fluorescence detection methods for intracellular caspase assay
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
WO2003002959A1 (en) * 2001-06-15 2003-01-09 Mj Research, Inc. Controller for a fluorometer
EP1409738A4 (en) * 2001-06-22 2005-03-30 Marshfield Clinic METHOD AND OLIGONUCLEOTIDES FOR DETECTING SALMONELLA SP., E.COLI O157: H7 AND LISTERIA MONOCYTOGENES
DE10132211A1 (de) * 2001-06-27 2003-01-16 Epigenomics Ag Nachweis spezifischer Dinukleotide in DNA-Proben durch Fluoreszenzresonanzenergietransfer (FRET)
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
DE10132785A1 (de) * 2001-07-06 2003-01-16 Clondiag Chip Tech Gmbh Verfahren zum Nachweis von in einer Polymerase-Kettenreaktion amplifizierten Nukleinsäuremolekülen
EP1275735A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
US7226732B2 (en) * 2001-07-16 2007-06-05 Cepheid Methods, apparatus, and computer programs for verifying the integrity of a probe
AU2002313676A1 (en) * 2001-07-16 2003-03-03 Idaho Technology, Inc. Thermal cycling system and method of use
US6872529B2 (en) * 2001-07-25 2005-03-29 Affymetrix, Inc. Complexity management of genomic DNA
US6979541B1 (en) 2001-07-26 2005-12-27 University Of Utah Research Foundation Methods for identifying chromosomal aneuploidy
US7188030B2 (en) * 2001-08-21 2007-03-06 Applera Corporation Automatic threshold setting for quantitative polymerase chain reaction
ATE348192T1 (de) * 2001-08-23 2007-01-15 Merck & Co Inc Multiplex-hpv-pcr-fluoreszenzassays unter verwendung mehrerer fluorophore
ES2269614T3 (es) * 2001-08-31 2007-04-01 The University Of Utah Research Foundation Cuantificacion de genes en tiempo real con estandares internos.
CN1262351C (zh) * 2001-09-11 2006-07-05 伊库姆有限公司 试样容器
US20030082563A1 (en) * 2001-10-15 2003-05-01 Bell Constance A. Detection of bacillus anthracis
US20030165859A1 (en) 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
EP1308524B1 (en) * 2001-11-02 2006-05-31 Roche Diagnostics GmbH Detection of variola virus
AU2002351193A1 (en) * 2001-11-28 2003-06-10 Mj Bioworks Incorporated Parallel polymorphism scoring by amplification and error correction
US7352694B1 (en) * 2001-12-14 2008-04-01 Applied Micro Circuits Corporation System and method for tolerating data link faults in a packet communications switch fabric
US8418129B1 (en) 2001-12-14 2013-04-09 Qualcomm Incorporated Method for automatically generating code to define a system of hardware elements
US7424013B1 (en) * 2001-12-20 2008-09-09 Applied Micro Circuits Corporation System and method for granting arbitrated bids in the switching of information
US20040063109A2 (en) 2002-01-25 2004-04-01 Applera Corporation Single-tube, ready-to-use assay kits, and methods using same
US7228237B2 (en) * 2002-02-07 2007-06-05 Applera Corporation Automatic threshold setting and baseline determination for real-time PCR
US7373253B2 (en) 2002-02-12 2008-05-13 Idaho Technology Multi-test analysis of real-time nucleic acid amplification
US6593093B1 (en) 2002-02-20 2003-07-15 Mayo Foundation For Medical Education And Research Detection of group a Streptococcus
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US20050065330A1 (en) * 2002-04-24 2005-03-24 The Cleveland Clinic Foundation Identification of Histoplasma capsulatum using a PCR assay
US7297484B2 (en) * 2002-04-26 2007-11-20 Idaho Technology Characterization of single stranded nucleic acids by melting analysis of secondary structure using double strand-specific nucleic acid dye
AUPS205802A0 (en) * 2002-05-01 2002-06-06 Bio-Molecular Holdings Pty Limited Improved cycling device and method
US7785776B2 (en) * 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
US20030215814A1 (en) * 2002-05-17 2003-11-20 Cockerill Franklin R. Detection of Shiga toxin- or Shiga-like toxin-producing organisms
AUPS267802A0 (en) * 2002-05-30 2002-06-20 Bio-Molecular Holdings Pty Limited Improved dna amplification apparatus and method
JP4457001B2 (ja) * 2002-05-31 2010-04-28 セクレタリー・デパートメント・オブ・アトミック・エナジー ドナー／アクセプター部分が相補鎖上となる標的核酸検出のｍｅｔ／ｆｒｅｔに基づく方法
EP1513958B1 (en) * 2002-06-14 2011-08-10 Gen-Probe Incorporated Compositions for detecting hepatitis b virus
US6896727B2 (en) * 2002-06-28 2005-05-24 Seh America, Inc. Method of determining nitrogen concentration within a wafer
US7083974B2 (en) * 2002-07-12 2006-08-01 Applera Corporation Rotatable sample disk and method of loading a sample disk
US20040219557A1 (en) * 2002-08-01 2004-11-04 Dobrowolski Steven F. Real time PCR assays to detect mutations in the biotinidase gene for newborn screening
US8277753B2 (en) * 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
US9394332B2 (en) * 2002-08-29 2016-07-19 Epigenomics Ag Method for bisulfite treatment
DE60329853D1 (de) * 2002-09-03 2009-12-10 Quanta Biosciences Inc Verbesserte zusammensetzungen und verfahren für die cdna-synthese
WO2004027023A2 (en) * 2002-09-17 2004-04-01 Pharmacia Corporation Preservation of rna and reverse transcriptase during automated liquid handling
MXPA05002934A (es) * 2002-09-18 2006-02-24 Univ Pennsylvania Composiciones, metodos y kits para deteccion de un antigeno en una celula y en una mezcla biologica.
US7074598B2 (en) 2002-09-25 2006-07-11 Mayo Foundation For Medical Education And Research Detection of vancomycin-resistant enterococcus spp.
US7365176B2 (en) 2002-09-26 2008-04-29 Mayo Foundation For Medical Education And Research Detection of Epstein-Barr virus
US7074599B2 (en) 2002-09-27 2006-07-11 Mayo Foundation For Medical Education And Research Detection of mecA-containing Staphylococcus spp.
WO2004031408A1 (en) * 2002-09-30 2004-04-15 F.Hoffmann-La Roche Ag Oligonucleotides for genotyping thymidylate synthase gene
US9850381B2 (en) * 2002-10-23 2017-12-26 University Of Utah Research Foundation Amplicon melting analysis with saturation dyes
ES2527068T3 (es) * 2002-10-23 2015-01-19 University Of Utah Research Foundation Análisis de fusión de amplicones con colorantes de saturación
EP1570079B1 (en) 2002-12-06 2010-07-21 Roche Diagnostics GmbH Multiplex assay detection of pathogenic organisms
US7718361B2 (en) 2002-12-06 2010-05-18 Roche Molecular Systems, Inc. Quantitative test for bacterial pathogens
US8275554B2 (en) * 2002-12-20 2012-09-25 Caliper Life Sciences, Inc. System for differentiating the lengths of nucleic acids of interest in a sample
WO2004061132A1 (en) * 2002-12-20 2004-07-22 Stratagene Compositions and methods for polynucleotide detection
US7560231B2 (en) * 2002-12-20 2009-07-14 Roche Molecular Systems, Inc. Mannitol and glucitol derivatives
US20050042639A1 (en) * 2002-12-20 2005-02-24 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA length
EP1587940A4 (en) 2002-12-20 2006-06-07 Caliper Life Sciences Inc SINGLE MOLECULAR AMPLIFICATION AND DNA PROOF
CA2521999A1 (en) * 2002-12-20 2004-09-02 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
US20060094108A1 (en) * 2002-12-20 2006-05-04 Karl Yoder Thermal cycler for microfluidic array assays
WO2004067545A1 (en) * 2003-01-29 2004-08-12 Roche Diagnostics Gmbh Improved method for bisulfite treatment
WO2004071155A2 (en) * 2003-02-05 2004-08-26 Amersham Biosciences Corp Solid phase sequencing
JP4632262B2 (ja) * 2003-02-05 2011-02-16 アイキューム，インク． サンプルの処理
CA2516299A1 (en) * 2003-02-18 2004-09-02 Applera Corporation Compositions and methods for multiplex analysis of polynucleotides
US7282331B2 (en) * 2003-03-18 2007-10-16 Advandx, Inc. Method for improved specificity in probe based assays
EP2390352A1 (en) 2003-03-18 2011-11-30 Quantum Genetics Ireland Limited Systems and methods for improving protein and milk production of dairy herds
US20040191776A1 (en) * 2003-03-21 2004-09-30 Pei-Jer Chen Method for genotyping and quantifying Hepatitis B Virus
GB2399776A (en) * 2003-03-24 2004-09-29 Pa Knowledge Ltd Cyclical heating and cooling device and associated methods
JP4782668B2 (ja) 2003-03-31 2011-09-28 エフ．ホフマン−ラ ロシュ アーゲー 日本脳炎ウィルス血清グループのメンバーを包含する一定のフラビウィルスを検出するための組成物及び方法
ATE550099T1 (de) 2003-04-04 2012-04-15 Hoffmann La Roche Verbessertes system für mehrfarbige echtzeit-pcr
CA2463719A1 (en) * 2003-04-05 2004-10-05 F. Hoffmann-La Roche Ag Nucleotide analogs with six membered rings
WO2004094986A2 (en) * 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
WO2005030027A2 (en) * 2003-04-29 2005-04-07 The Cleveland Clinic Foundation Salmonella detection identification
US7148043B2 (en) 2003-05-08 2006-12-12 Bio-Rad Laboratories, Inc. Systems and methods for fluorescence detection with a movable detection module
CA2523040C (en) 2003-05-23 2012-01-17 Bio-Rad Laboratories, Inc. Localized temperature control for spatial arrays of reaction media
US20070059701A1 (en) * 2003-06-30 2007-03-15 Astrazeneca Ab Fluorescence assays for nucleic acid polymerase activity
EP1493822A1 (de) * 2003-07-02 2005-01-05 Labor Becker, Olgemoeller & Kollegen GbR Detektionsverfahren für Nucleinsäuren mit interner Amplifikationskontrolle
GB0317343D0 (en) * 2003-07-24 2003-08-27 Medical Biosystems Ltd Polynucleotide sequencing
EP1502958A1 (en) * 2003-08-01 2005-02-02 Roche Diagnostics GmbH New detection format for hot start real time polymerase chain reaction
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
US7788039B2 (en) 2003-09-25 2010-08-31 Roche Molecular Systems, Inc. Quantitation of nucleic acids using growth curves
EP1682898A2 (en) * 2003-11-04 2006-07-26 Roche Diagnostics GmbH Method for distinguishing aml subtypes with recurring genetic aberrations
WO2005043161A2 (en) * 2003-11-04 2005-05-12 Roche Diagnostics Gmbh Method for distinguishing leukemia subtypes
EP1533618A1 (en) * 2003-11-04 2005-05-25 Ludwig-Maximilians-Universität München Method for distinguishing prognostically definable AML
US20070292970A1 (en) * 2003-11-04 2007-12-20 Martin Dugas Method for Distinguishing Aml-Specific Flt3 Length Mutations From Tkd Mutations
US20070212734A1 (en) * 2003-11-04 2007-09-13 Martin Dugas Method for Distinguishing T(11Q23)/Mll-Positive Leukemias From t(11Q23)/Mll Negative Leukemia
WO2005045437A2 (en) * 2003-11-04 2005-05-19 Roche Diagnostics Gmbh Method for distinguishing immunologically defined all subtypes
EP1530046A1 (en) * 2003-11-04 2005-05-11 Ludwig-Maximilians-Universität München Method for distinguishing AML subtypes with aberrant and prognostically intermediate karyotypes
WO2005043167A2 (en) * 2003-11-04 2005-05-12 Roche Diagnostic Gmbh Method for distinguishing aml subtypes with differents gene dosages
EP1682902A2 (en) * 2003-11-04 2006-07-26 Roche Diagnostics GmbH Method for distinguishing mll-ptd-positive aml from other aml subtypes
EP1682905A2 (en) * 2003-11-04 2006-07-26 Roche Diagnostics GmbH Method for distinguishing cbf-positive aml subtypes from cbf-negative aml subtypes
WO2005045068A1 (de) * 2003-11-06 2005-05-19 Dual Juerg Chemische analyse mit dynamischer viskosimetrie
US7427475B2 (en) * 2003-11-18 2008-09-23 Mayo Foundation For Medical Education And Research Detection of group B streptococcus
EP1689764B1 (en) * 2003-11-19 2013-01-02 AlleLogic Biosciences Corporation Oligonucleotides labeled with a plurality of fluorophores
US7582452B2 (en) * 2003-11-24 2009-09-01 Life Genetics Lab, Llc Assay for species sources
CA2494571C (en) 2003-12-02 2010-02-09 F.Hoffmann-La Roche Ag Oligonucleotides containing molecular rods
JP4435174B2 (ja) 2003-12-02 2010-03-17 エフ．ホフマン−ラ ロシュ アーゲー 重亜硫酸塩処理の改良された方法
US8697433B2 (en) * 2003-12-10 2014-04-15 Samsung Electronics Co., Ltd. Polymerase chain reaction (PCR) module and multiple PCR system using the same
US20050130213A1 (en) * 2003-12-10 2005-06-16 Tom Morrison Selective ligation and amplification assay
CA2559949C (en) * 2004-02-10 2014-04-08 F. Hoffmann-La Roche Ag New primers and probes for the detection of parvovirus b19
US20050186606A1 (en) * 2004-02-11 2005-08-25 Schroeder Benjamin G. Methods and compositions for detecting nucleic acids
EP1718770B1 (en) 2004-02-19 2011-05-25 The Governors of the University of Alberta Leptin promoter polymorphisms and uses thereof
US20050250134A1 (en) * 2004-03-02 2005-11-10 Dawei Sheng Fluorescent energy transfer labeled nucleic acid substrates and methods of use thereof
EP1753873A4 (en) * 2004-03-05 2008-11-05 Ohio Med College METHOD AND COMPOSITIONS FOR THE ASSESSMENT OF NUCLEIC ACIDS AND ALLELES
EP1735097B1 (en) 2004-03-12 2016-11-30 Life Technologies Corporation Nanoliter array loading
US7445898B2 (en) 2004-04-01 2008-11-04 Bio-Rad Laboratories, Inc. Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity
US7476502B2 (en) * 2004-04-12 2009-01-13 Willey James C Methods and compositions for assaying analytes
EP1745120A4 (en) 2004-04-16 2010-04-14 Spartan Bioscience Inc SYSTEM FOR RAPID AMPLIFICATION AND DETECTION OF NUCLEIC ACIDS
US7387887B2 (en) * 2004-04-20 2008-06-17 University Of Utah Research Foundation Nucleic acid melting analysis with saturation dyes
US9657347B2 (en) 2004-04-20 2017-05-23 University of Utah Research Foundation and BioFire Defense, LLC Nucleic acid melting analysis with saturation dyes
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
US20050250112A1 (en) * 2004-05-07 2005-11-10 Padmabandu Gothami A Nucleic acids, compositions, methods, and kits for detecting mycoplasma and acholeplasma species
US7494791B2 (en) * 2004-05-13 2009-02-24 Nanobiosym, Inc. Nano-PCR: methods and devices for nucleic acid amplification and detection
DE102004025538A1 (de) * 2004-05-25 2005-12-22 Advalytix Ag Temperierverfahren und -vorrichtung für die Temperaturbehandlung kleiner Flüssigkeitsmengen
EP1771563A2 (en) * 2004-05-28 2007-04-11 Ambion, Inc. METHODS AND COMPOSITIONS INVOLVING MicroRNA
EP1605060A1 (en) * 2004-06-10 2005-12-14 Biodynamics S.R.L. Methods and reaction mixture reagent for increasing the specificity and fidelity of polymerase reactions
CA2566336C (en) * 2004-06-25 2014-05-13 E.I. Du Pont De Nemours And Company Dna sequences for the detection of and differentation amongst pathogenic e.coli
EP1610170A1 (en) * 2004-06-25 2005-12-28 Sony Deutschland GmbH A method of applying a particle film to create a surface having light-diffusive and/or reduced glare properties
GB0416944D0 (en) 2004-07-29 2004-09-01 Lumora Ltd Method for determining the amount of template nucleic acid present in a sample
TWI349704B (en) * 2004-08-10 2011-10-01 Food And Drug Administration Dept Of Health A method for rapidly detecting quinolone-resistant salmonella spp. and the probes and primers utilized therein
WO2006020933A2 (en) * 2004-08-13 2006-02-23 Stratagene California Dual labeled fluorescent probes
EP1632578A1 (en) 2004-09-03 2006-03-08 Roche Diagnostics GmbH DNA decontamination method
US20100003241A1 (en) 2004-09-07 2010-01-07 Patrick Holt Agents for treatment or prevention of an allergic disorder
EP1634965B1 (en) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Real time PCR with the addition of pyrophosphatase
US20060051796A1 (en) * 2004-09-09 2006-03-09 Inga Boell Real time PCR with the addition of pyrophosphatase
AU2005286876A1 (en) * 2004-09-20 2006-03-30 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Multiple mode multiplex reaction quenching method
EP1791982B1 (en) 2004-09-21 2011-01-05 Life Technologies Corporation TWO-COLOR REAL-TIME/END-POINT QUANTITATION OF MICRORNAS (MIRNAs)
CA2584267A1 (en) * 2004-10-07 2006-04-20 Chronix Biomedical Detection of nucleic acids to assess risk for creutzfeldt-jakob disease
US20060078894A1 (en) * 2004-10-12 2006-04-13 Winkler Matthew M Methods and compositions for analyzing nucleic acids
EP2927238B1 (en) * 2004-10-18 2018-01-17 Brandeis University Methods for LATE amplification and sequencing of a nucleic acid
US20060194222A1 (en) * 2004-10-20 2006-08-31 Stratagene California Triplex probe compositions and methods for polynucleotide detection
EP2316927B1 (en) 2004-10-21 2014-11-05 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Kaspp (lrrk2) gene, its production and use for the detection and treatment of neurodegenerative disorders
CN101068932B (zh) * 2004-10-27 2013-02-13 塞弗德公司 封闭系统多阶段核酸扩增反应
EP1809765A2 (en) * 2004-11-04 2007-07-25 Roche Diagnostics GmbH Classification of acute myeloid leukemia
WO2008073922A2 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Functions and targets of let-7 micro rnas
ES2503739T3 (es) 2004-11-12 2014-10-07 Asuragen, Inc. Procedimientos y composiciones que implican miARN y moléculas inhibidoras de miARN
CA2594223C (en) * 2005-01-03 2016-03-15 The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primer for nucleic acid detection
EP2208796B1 (en) 2005-01-06 2014-06-18 Applied Biosystems, LLC Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
US7315376B2 (en) * 2005-01-07 2008-01-01 Advanced Molecular Systems, Llc Fluorescence detection system
DE602005000877T2 (de) 2005-01-18 2007-12-20 Roche Diagnostics Gmbh Fluoreszenzabbildung mittels Telezentrizität
ES2284087T3 (es) 2005-01-18 2007-11-01 F. Hoffmann-La Roche Ag Generacion de imagenes de señales de fluorescencia mediante opticas telecentricas de excitacion y de generacion de imagenes.
US8470608B2 (en) * 2008-05-20 2013-06-25 Rapid Pathogen Screening, Inc Combined visual/fluorescence analyte detection test
US8669052B2 (en) 2008-06-10 2014-03-11 Rapid Pathogen Screening, Inc. Lateral flow nucleic acid detector
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US9464310B2 (en) * 2005-02-10 2016-10-11 Medical Diagnostic Laboratories, Llc Integrated method for collection and maintenance of detectability of a plurality of microbiological agents in a single clinical sample and for handling a plurality of samples for reporting a sum of diagnostic results for each sample
US20060178838A1 (en) * 2005-02-10 2006-08-10 Adelson Martin E Method of receiving and handling a plurality of clinical samples for reporting a sum of diagnostic results for each sample
US20060246423A1 (en) * 2005-02-10 2006-11-02 Adelson Martin E Method and kit for the collection and maintenance of the detectability of a plurality of microbiological species in a single gynecological sample
US20060188891A1 (en) * 2005-02-23 2006-08-24 Bickmore William D Jr Methods and apparatus for controlling DNA amplification
JP4398886B2 (ja) * 2005-03-07 2010-01-13 ソニー株式会社 通信端末装置、通信システム、通信方法、およびプログラム
EP1930730B1 (en) 2005-03-10 2019-08-14 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes
EP1700922B1 (en) 2005-03-10 2016-08-24 Roche Diagnostics GmbH 3-Substituted 5-Nitroindole derivatives and labeled oligonucleotide probes containing them
US7547516B2 (en) 2005-03-10 2009-06-16 Gen-Probe Incorporated Method for reducing the presence of amplification inhibitors in a reaction receptacle
US7759469B2 (en) 2005-03-10 2010-07-20 Roche Diagnostics Operations, Inc. Labeling reagent
US7680604B2 (en) 2005-03-11 2010-03-16 Roche Molecular Systems, Inc. PCR elbow determination by rotational transform after zero slope alignment
US7601498B2 (en) 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
US7776567B2 (en) 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7456281B2 (en) * 2005-04-20 2008-11-25 Idaho Technology, Inc. Nucleic acid melting analysis with saturation dyes
WO2006116276A2 (en) * 2005-04-28 2006-11-02 Merck & Co., Inc. Real-time hpv pcr assays
CA2607369A1 (en) * 2005-05-02 2006-11-09 Stratagene California Oligonucleotide probe/primer compositions and methods for polynucleotide detection
US7569695B2 (en) * 2005-05-24 2009-08-04 Enzo Life Sciences, Inc. Dyes for the detection or quantification of desirable target molecules
US8362250B2 (en) 2005-05-24 2013-01-29 Enzo Biochem, Inc. Fluorescent dyes and compounds, methods and kits useful for identifying specific organelles and regions in cells of interest
US7737281B2 (en) * 2005-05-24 2010-06-15 Enzo Life Sciences, Inc. C/O Enzo Biochem, Inc. Purine based fluorescent dyes
US8357801B2 (en) 2005-05-24 2013-01-22 Enzo Life Sciences, Inc. Labeling of target molecules, identification of organelles and other applications, novel compositions, methods and kits
US20070077570A1 (en) * 2005-05-31 2007-04-05 Applera Corporation Multiplexed amplification of short nucleic acids
US20060281099A1 (en) * 2005-06-14 2006-12-14 Bio-Rad Laboratories, Inc. Q-melt for polymorphism detection
CN101466847B (zh) 2005-06-15 2014-02-19 考利达基因组股份有限公司 用于遗传和化学分析的单分子阵列
US20060286590A1 (en) * 2005-06-20 2006-12-21 Applera Corporation. Cis-regulatory modules
US20060292578A1 (en) 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
GB0514889D0 (en) 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
JP2009506759A (ja) * 2005-09-01 2009-02-19 コーベット ライフ サイエンス ピーティーワイ リミテッド 核酸の増幅、定量化、及び同定の方法。
US7569367B2 (en) 2005-09-07 2009-08-04 Roche Diagnostics Operations, Inc. Nucleic acid preparation from whole blood for use in diagnosis of transmissible spongiform encephalopathy
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
US7991558B2 (en) 2005-09-29 2011-08-02 Roche Molecular Systems, Inc. Systems and methods for determining real-time PCR cycle thresholds using cluster analysis
US7991561B2 (en) 2005-09-29 2011-08-02 Roche Molecular Systems, Inc. Ct determination by cluster analysis with variable cluster endpoint
US20070212704A1 (en) 2005-10-03 2007-09-13 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
WO2007044245A2 (en) 2005-10-07 2007-04-19 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
WO2007047606A2 (en) * 2005-10-17 2007-04-26 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Electrokinetic thermal cycler and reactor
US7781165B2 (en) 2005-10-19 2010-08-24 Roche Diagnostics Operations, Inc. Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification
WO2007120208A2 (en) * 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
KR101542677B1 (ko) 2005-11-29 2015-08-06 캠브리지 엔터프라이즈 리미티드 유방암에 대한 마커
EP1798291B1 (en) * 2005-12-13 2011-05-18 Institut Curie Methods and compositions for assaying mutations and/or large scale alterations in nucleic acids and their uses in diagnosis of genetic diseases and cancers
JP5374679B2 (ja) * 2005-12-14 2013-12-25 エピゲノミクス アーゲー 重亜硫酸処理のための新規方法
EP1798542A1 (en) * 2005-12-19 2007-06-20 Roche Diagnostics GmbH Analytical method and instrument
EP1798650A1 (en) 2005-12-19 2007-06-20 Roche Diagnostics GmbH Analytical method and instrument
US7844403B2 (en) 2005-12-20 2010-11-30 Roche Molecular Systems, Inc. Temperature step correction with double sigmoid Levenberg-Marquardt and robust linear regression
US7991562B2 (en) 2005-12-20 2011-08-02 Roche Molecular Systems, Inc. PCR elbow determination using curvature analysis of a double sigmoid
US7668663B2 (en) 2005-12-20 2010-02-23 Roche Molecular Systems, Inc. Levenberg-Marquardt outlier spike removal method
US7680868B2 (en) 2005-12-20 2010-03-16 Roche Molecular Systems, Inc. PCR elbow determination by use of a double sigmoid function curve fit with the Levenburg-Marquardt algorithm and normalization
US7981606B2 (en) * 2005-12-21 2011-07-19 Roche Molecular Systems, Inc. Control for nucleic acid testing
AU2007207544A1 (en) 2006-01-17 2007-07-26 Health Research, Inc. Heteroduplex tracking assay
US20090305238A1 (en) * 2006-01-23 2009-12-10 Applera Corporation Microarray Microcard
US7785786B2 (en) * 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
WO2007092538A2 (en) * 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
GB0603190D0 (en) * 2006-02-16 2006-03-29 Enigma Diagnostics Ltd Detection system
CA2638854C (en) 2006-02-27 2012-05-15 F. Hoffmann-La Roche Ag Pcr hot start by magnesium sequestration
MX2008011185A (es) * 2006-03-01 2008-09-10 Perlegen Sciences Inc Marcadores para adiccion.
US20110143344A1 (en) * 2006-03-01 2011-06-16 The Washington University Genetic polymorphisms and substance dependence
RU2394915C2 (ru) * 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
US8778637B2 (en) 2006-03-28 2014-07-15 Canon U.S. Life Sciences, Inc. Method and apparatus for applying continuous flow and uniform temperature to generate thermal melting curves in a microfluidic device
AU2007233252C1 (en) 2006-03-29 2013-05-23 Boehringer Ingelheim Animal Health USA Inc. Vaccine against Streptococci
EP2002013A4 (en) 2006-04-04 2010-05-05 Labonnet Ltd ASSESSMENT OF REACTION KINETICS COMPATIBILITY BETWEEN POLYMERASE CHAIN REACTIONS
US20070238108A1 (en) * 2006-04-07 2007-10-11 Agilent Technologies, Inc. Validation of comparative genomic hybridization
US20070238095A1 (en) * 2006-04-11 2007-10-11 Mayo Foundation For Medical Education And Research , A Minnesota Corporation Detection of Influenza A Virus
US20070238093A1 (en) * 2006-04-11 2007-10-11 Espy Mark J Detection of influenza A virus
WO2007124105A2 (en) 2006-04-21 2007-11-01 Nanobiosym, Inc. Single-molecule platform for drug discovery: methods and apparatuses for drug discovery, including discovery of anticancer and antiviral agents
WO2007127992A2 (en) 2006-04-28 2007-11-08 Igor Kutyavin Use of base-modified deoxynucleoside triphosphates
US8900828B2 (en) 2006-05-01 2014-12-02 Cepheid Methods and apparatus for sequential amplification reactions
US8232091B2 (en) * 2006-05-17 2012-07-31 California Institute Of Technology Thermal cycling system
EP2535427A3 (en) * 2006-05-17 2013-04-24 California Institute of Technology Thermal cycling system
US8637436B2 (en) 2006-08-24 2014-01-28 California Institute Of Technology Integrated semiconductor bioarray
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
EP2029775B1 (en) * 2006-06-05 2014-10-15 California Institute Of Technology Real time micro arrays
US8119352B2 (en) * 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
US7629124B2 (en) 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
WO2008021010A2 (en) * 2006-08-07 2008-02-21 Stratagene California Methods for real-time quantitative pcr
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US9481912B2 (en) 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
US8097419B2 (en) 2006-09-12 2012-01-17 Longhorn Vaccines & Diagnostics Llc Compositions and method for rapid, real-time detection of influenza A virus (H1N1) swine 2009
US8080645B2 (en) 2007-10-01 2011-12-20 Longhorn Vaccines & Diagnostics Llc Biological specimen collection/transport compositions and methods
EP1900824A1 (en) * 2006-09-14 2008-03-19 Deutsches Krebsforschungszentrum Stiftung Des Öffentlichen Rechts Gene expression signature for the prognosis, diagnosis and therapy of prostate cancer and uses thereof
AU2007299748A1 (en) * 2006-09-19 2008-03-27 Asuragen, Inc. miR-15, miR-26, miR -31,miR -145, miR-147, miR-188, miR-215, miR-216 miR-331, mmu-miR-292-3p regulated genes and pathways as targets for therapeutic intervention
US20100297026A1 (en) * 2006-11-02 2010-11-25 Doyle Gerald V Imaging of Activated Vascular Endothelium Using Immunomagnetic MRI Contrast Agents
US9114398B2 (en) 2006-11-29 2015-08-25 Canon U.S. Life Sciences, Inc. Device and method for digital multiplex PCR assays
US20080131878A1 (en) * 2006-12-05 2008-06-05 Asuragen, Inc. Compositions and Methods for the Detection of Small RNA
CA2671194A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Mir-20 regulated genes and pathways as targets for therapeutic intervention
CN101622349A (zh) * 2006-12-08 2010-01-06 奥斯瑞根公司 作为治疗性干预靶标的miR-21调节的基因和途径
CA2671294A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Mir-21 regulated genes and pathways as targets for therapeutic intervention
BRPI0721095B1 (pt) 2006-12-13 2015-09-29 Luminex Corporation Sistemas e métodos para a análise multíplex de pcr em tempo real
WO2008074101A2 (en) 2006-12-21 2008-06-26 Agriculture Victoria Services Pty Limited Artificial selection method and reagents
US8535888B2 (en) * 2006-12-29 2013-09-17 Mayo Foundation For Medical Education And Research Compositions and methods for detecting methicillin-resistant S. aureus
US20090175827A1 (en) * 2006-12-29 2009-07-09 Byrom Mike W miR-16 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION
US8247171B2 (en) * 2007-02-01 2012-08-21 Abacus Diagnostica Oy Method for detection of presence of target polynucleotide in samples
ES2665690T3 (es) 2007-02-02 2018-04-26 Genera Biosystems Limited Generación de moléculas de ácido nucleico
US20100190167A1 (en) * 2007-02-14 2010-07-29 Genisphere Inc. Methods, Reagents and Kits for Detection of Nucleic Acid Molecules
JP4942508B2 (ja) * 2007-02-20 2012-05-30 アークレイ株式会社 ａｂｌ遺伝子変異の検出用プローブおよびその用途
US9475051B2 (en) * 2007-02-27 2016-10-25 Sony Corporation Nucleic acid amplifier
GB0703996D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Nucleic acid detection
GB0703997D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Methods for detecting nucleic sequences
ES2582602T3 (es) 2007-03-28 2016-09-14 Signal Diagnostics Sistema y método de análisis de alta resolución de ácidos nucleicos para detectar variaciones de secuencia
JP5191041B2 (ja) 2007-04-05 2013-04-24 エフ．ホフマン−ラ ロシュ アーゲー 急速ワンステップｒｔ−ｐｃｒ
US8017337B2 (en) 2007-04-19 2011-09-13 Molecular Detection, Inc. Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria
US20090232893A1 (en) * 2007-05-22 2009-09-17 Bader Andreas G miR-143 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION
CA2689974A1 (en) * 2007-06-08 2008-12-18 Asuragen, Inc. Mir-34 regulated genes and pathways as targets for therapeutic intervention
EP2167964B1 (de) * 2007-06-13 2015-03-11 Attomol GmbH Molekulare Diagnostika Verfahren zur durchführung und auswertung von mix&measure assays für die messung von reaktionskinetiken sowie von konzentrationen und affinitäten von analyten im multiplexformat
DE102007031137A1 (de) * 2007-06-13 2008-12-18 Attomol Gmbh Molekulare Diagnostika Verfahren und Sonden/Primärsystem zum "real time" Nachweis eines Nukleinsäuretargets
CN103157400B (zh) 2007-06-21 2014-12-24 简·探针公司 用于将容器暴露于多个热区的仪器和方法
EP2395113A1 (en) 2007-06-29 2011-12-14 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
US20090023138A1 (en) * 2007-07-17 2009-01-22 Zila Biotechnology, Inc. Oral cancer markers and their detection
US20090055243A1 (en) 2007-08-21 2009-02-26 Jayson Lee Lusk Systems and methods for predicting a livestock marketing method
US11041215B2 (en) 2007-08-24 2021-06-22 Longhorn Vaccines And Diagnostics, Llc PCR ready compositions and methods for detecting and identifying nucleic acid sequences
US8386184B2 (en) 2007-08-28 2013-02-26 Becton, Dickinson And Company Systems and methods for determining an amount of starting reagent using the polymerase chain reaction
US8380457B2 (en) 2007-08-29 2013-02-19 Canon U.S. Life Sciences, Inc. Microfluidic devices with integrated resistive heater electrodes including systems and methods for controlling and measuring the temperatures of such heater electrodes
DE102007041864B4 (de) 2007-09-04 2012-05-03 Sirs-Lab Gmbh Verfahren zum Nachweis von Bakterien und Pilzen
US8361714B2 (en) * 2007-09-14 2013-01-29 Asuragen, Inc. Micrornas differentially expressed in cervical cancer and uses thereof
US20090119020A1 (en) 2007-09-25 2009-05-07 Roche Molecular Systems, Inc. Pcr elbow determination using quadratic test for curvature analysis of a double sigmoid
JP5432154B2 (ja) 2007-09-28 2014-03-05 スリーエム イノベイティブ プロパティズ カンパニー 核酸検出の二重オリゴヌクレオチド法
US20090186015A1 (en) * 2007-10-18 2009-07-23 Latham Gary J Micrornas differentially expressed in lung diseases and uses thereof
FR2922897B1 (fr) * 2007-10-25 2009-11-27 Bio Rad Pasteur Mesure d'une population d'acides nucleiques,en particulier par pcr en temps reel.
WO2009055039A1 (en) * 2007-10-25 2009-04-30 Canon U.S. Life Sciences, Inc High-resolution melting analysis
JP2011501965A (ja) 2007-10-30 2011-01-20 コンプリート・ゲノミックス・インコーポレーテッド 核酸のハイ・スループット塩基配列決定のための装置
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
US20090137058A1 (en) * 2007-11-28 2009-05-28 General Electric Company Chemical and biological detection method and device based on measurements of fluorescence and reflectivity
US8071562B2 (en) * 2007-12-01 2011-12-06 Mirna Therapeutics, Inc. MiR-124 regulated genes and pathways as targets for therapeutic intervention
US9551026B2 (en) 2007-12-03 2017-01-24 Complete Genomincs, Inc. Method for nucleic acid detection using voltage enhancement
US20090162863A1 (en) * 2007-12-13 2009-06-25 Hitachi High-Technologies Corporation Nucleic acid detection probe
US20090192114A1 (en) * 2007-12-21 2009-07-30 Dmitriy Ovcharenko miR-10 Regulated Genes and Pathways as Targets for Therapeutic Intervention
US8364415B2 (en) * 2007-12-26 2013-01-29 Arkray, Inc. Melting curve analyzing method and melting curve analyzing device
JP5542439B2 (ja) * 2007-12-26 2014-07-09 アークレイ株式会社 核酸増幅判定方法および核酸増幅判定装置
DE102007063102B4 (de) 2007-12-28 2022-02-10 Robert Bosch Gmbh Verfahren zur Erfassung eines periodisch pulsierenden Betriebsparameters
EP2238459B1 (en) * 2008-01-23 2019-05-08 Roche Diagnostics GmbH Integrated instrument performing synthesis and amplification
EP2260110B1 (en) * 2008-02-08 2014-11-12 Asuragen, INC. miRNAs DIFFERENTIALLY EXPRESSED IN LYMPH NODES FROM CANCER PATIENTS
WO2009100215A2 (en) 2008-02-08 2009-08-13 Mayo Foundation For Medical Education And Research Detection of clostridium difficile
WO2009103003A2 (en) * 2008-02-15 2009-08-20 Bio-Rad Laboratories, Inc. Scanning fluorescent reader with diffuser system
US8153374B2 (en) 2008-03-03 2012-04-10 Heatflow Technologies, Inc. Heat flow polymerase chain reaction methods
WO2009111072A1 (en) * 2008-03-05 2009-09-11 Clean Earth Technologies, Llc Multiplexed pcr-coupled pna labeled beads flow cytometric assay for simultaneous detection of multiple biological agents
CA2658520C (en) * 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
US20090253780A1 (en) * 2008-03-26 2009-10-08 Fumitaka Takeshita COMPOSITIONS AND METHODS RELATED TO miR-16 AND THERAPY OF PROSTATE CANCER
EP2276859B1 (en) * 2008-04-08 2011-12-14 Roche Diagnostics GmbH Detection of pcr products in gel electrophoresis
EP2108699B1 (en) 2008-04-08 2014-06-25 F.Hoffmann-La Roche Ag Analytical processing and detection device
US20090258928A1 (en) * 2008-04-08 2009-10-15 Asuragen, Inc. Methods and compositions for diagnosing and modulating human papillomavirus (hpv)
EP2285960B1 (en) 2008-05-08 2015-07-08 Asuragen, INC. Compositions and methods related to mir-184 modulation of neovascularization or angiogenesis
US20130196310A1 (en) 2008-05-20 2013-08-01 Rapid Pathogen Screening, Inc. Method and Device for Combined Detection of Viral and Bacterial Infections
US8815609B2 (en) 2008-05-20 2014-08-26 Rapid Pathogen Screening, Inc. Multiplanar lateral flow assay with diverting zone
US9068981B2 (en) 2009-12-04 2015-06-30 Rapid Pathogen Screening, Inc. Lateral flow assays with time delayed components
US8962260B2 (en) 2008-05-20 2015-02-24 Rapid Pathogen Screening, Inc. Method and device for combined detection of viral and bacterial infections
US8609433B2 (en) 2009-12-04 2013-12-17 Rapid Pathogen Screening, Inc. Multiplanar lateral flow assay with sample compressor
ES2439951T3 (es) 2008-06-06 2014-01-27 F. Hoffmann-La Roche Ag Detección y cuantificación multiplex de ácidos nucleicos microbianos controlada de forma interna
US20110086359A1 (en) 2008-06-10 2011-04-14 Rapid Pathogen Screening, Inc. Lateral flow assays
EP2138588A1 (en) * 2008-06-23 2009-12-30 Koninklijke Philips Electronics N.V. Melting curve measurement during amplification
US8097412B2 (en) 2008-07-12 2012-01-17 Biodiagnostics, Inc. DNA-based test for detection of annual and intermediate ryegrass
DE102008040513B4 (de) * 2008-07-17 2010-08-26 BAM Bundesanstalt für Materialforschung und -prüfung Verwendung einer langwellig emittierenden Cyaninverbindung als NIR-Fluoreszenzstandard und Kit zur Kalibrierung von Photolumineszenzmesssystemen
EP2148187A1 (de) 2008-07-25 2010-01-27 Roche Diagnostics GmbH Anregungs- und Abbildungsoptik für die Fluoreszenzdetektion
GB0909333D0 (en) 2009-06-01 2009-07-15 Fu Guoliang Multiplex amplification and detection
CA2638458A1 (en) * 2008-07-31 2010-01-31 Spartan Bioscience Inc. Thermal recycling by positioning relative to fixed-temperature source
WO2010012464A1 (en) 2008-08-01 2010-02-04 Roche Diagnostics Gmbh Improved lysis and reverse transcription for mrna quantification
US20100055733A1 (en) * 2008-09-04 2010-03-04 Lutolf Matthias P Manufacture and uses of reactive microcontact printing of biomolecules on soft hydrogels
US9250249B2 (en) 2008-09-08 2016-02-02 Enzo Biochem, Inc. Autophagy and phospholipidosis pathway assays
US9334281B2 (en) * 2008-09-08 2016-05-10 Enzo Life Sciences, Inc. Fluorochromes for organelle tracing and multi-color imaging
CN102187215A (zh) 2008-09-09 2011-09-14 生物辐射实验室股份有限公司 基于回归的多级pcr分析系统
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
US8219324B2 (en) 2008-09-12 2012-07-10 Roche Molecular Systems, Inc. Real-time PCR elbow calling by equation-less algorithm
US9540686B2 (en) * 2008-09-18 2017-01-10 Canon U.S. Life Sciences, Inc. Systems and methods for the amplification of DNA
US8170804B2 (en) * 2008-10-17 2012-05-01 Roche Molecular Systems, Inc. Determination of melting temperatures of DNA
CA2682439A1 (en) 2008-10-17 2010-04-17 F. Hoffmann-La Roche Ag Cell monitoring and molecular analysis
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
US20100112630A1 (en) * 2008-11-03 2010-05-06 Scott Martell Boyette Methods for measuring microbiological content in aqueous media
US20100119454A1 (en) * 2008-11-03 2010-05-13 Ping Shen Use of the conserved Drosophila NPFR1 system for uncovering interacting genes and pathways important in nociception and stress response
US8691510B2 (en) 2008-11-07 2014-04-08 Sequenta, Inc. Sequence analysis of complex amplicons
EP4335932A3 (en) 2008-11-07 2024-06-26 Adaptive Biotechnologies Corporation Methods of monitoring conditions by sequence analysis
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US9394567B2 (en) 2008-11-07 2016-07-19 Adaptive Biotechnologies Corporation Detection and quantification of sample contamination in immune repertoire analysis
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US20100179213A1 (en) * 2008-11-11 2010-07-15 Mirna Therapeutics, Inc. Methods and Compositions Involving miRNAs In Cancer Stem Cells
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
US20110300544A1 (en) 2008-12-09 2011-12-08 Guoliang Fu Enhanced taqman probe based amplification
JP2012511927A (ja) 2008-12-17 2012-05-31 ライフ テクノロジーズ コーポレーション 対立遺伝子変種を検出するための方法、組成物、およびキット
CA2688174C (en) 2008-12-19 2018-08-07 F. Hoffmann-La Roche Ag Dry composition of reaction compounds with stabilized polymerase
US20100159452A1 (en) * 2008-12-22 2010-06-24 Roche Molecular Systems, Inc. Method For Detecting a Target Nucleic Acid in a Sample
US8206929B2 (en) 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
EP2382321A4 (en) 2009-01-08 2012-12-19 Bio Rad Laboratories METHODS AND COMPOSITIONS FOR ENHANCING THE EFFICACY OF NUCLEIC ACID AMPLIFICATION REACTIONS
EA020593B1 (ru) 2009-01-13 2014-12-30 Флуидигм Корпорейшн Анализ нуклеиновых кислот, полученных из единичных клеток
PL2387627T3 (pl) 2009-01-15 2016-10-31 Profilowanie odporności adaptacyjnej i wytwarzanie przeciwciał monoklonalnych
WO2010086410A1 (en) 2009-01-30 2010-08-05 Kantonsspital Aarau Ag Gene dosage analysis
WO2010088496A1 (en) 2009-01-30 2010-08-05 Gen-Probe Incorporated Systems and methods for detecting a signal and applying thermal energy to a signal transmission element
US9347092B2 (en) * 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
JP5457222B2 (ja) * 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US8606527B2 (en) * 2009-02-27 2013-12-10 Bio-Rad Laboratories, Inc. SNP detection by melt curve clustering
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
US9542526B2 (en) * 2009-03-10 2017-01-10 Canon U.S. Life Sciences, Inc. Method and system for temperature correction in thermal melt analysis
CN102428190B (zh) 2009-03-27 2014-02-26 生命技术公司 用于检测等位基因变体的方法、组合物和试剂盒
GB0905325D0 (en) 2009-03-30 2009-05-13 Selex Sensors & Airborne Sys Detection system
US20100279299A1 (en) * 2009-04-03 2010-11-04 Helixis, Inc. Devices and Methods for Heating Biological Samples
US20120028819A1 (en) 2009-04-07 2012-02-02 Koninklijke Philips Electronics N.V. Method for the detection and characterization of a toxinogenic clostridium difficile strain
WO2010118477A1 (en) 2009-04-17 2010-10-21 Molecular Plant Breeding Nominees Ltd Plant promoter operable in endosperm and uses thereof
WO2010133972A1 (en) 2009-05-22 2010-11-25 Population Genetics Technologies Ltd Sorting asymmetrically tagged nucleic acids by selective primer extension
EP2436777B1 (en) 2009-05-26 2015-08-19 Xiamen University Method for detecting variations in nucleic acid sequences
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
WO2010138187A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Scaffolded nucleic acid polymer particles and methods of making and using
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
JP2012531202A (ja) 2009-06-25 2012-12-10 フレッド ハチンソン キャンサー リサーチ センター 適応免疫を測定する方法
EP3018218B1 (en) 2009-07-21 2018-01-24 Gen-Probe Incorporated Methods for detection of nucleic acid sequences over an extended dynamic range
ES2562159T3 (es) 2009-08-20 2016-03-02 Population Genetics Technologies Ltd. Composiciones y métodos para el reordenamiento de ácido nucleico molecular
WO2011022589A2 (en) * 2009-08-20 2011-02-24 Mayo Foundation For Medical Education And Research Detection of enterovirus
US20110059453A1 (en) * 2009-08-23 2011-03-10 Affymetrix, Inc. Poly(A) Tail Length Measurement by PCR
FI20090306A0 (fi) 2009-08-25 2009-08-25 Qret Technologies Oy Erotusvapaa määritysmenetelmä
US8219366B2 (en) 2009-08-26 2012-07-10 Roche Molecular Sytems, Inc. Determination of elbow values for PCR for parabolic shaped curves
WO2011031377A1 (en) * 2009-09-09 2011-03-17 Helixis, Inc. Optical system for multiple reactions
US8815515B1 (en) 2009-09-24 2014-08-26 University Of Utah Research Foundation Methods, compositions, and kits for rare allele detection
WO2011053871A2 (en) * 2009-10-30 2011-05-05 Life Technologies Corporation Multi-primer assay for mycoplasma detection
US8524450B2 (en) 2009-10-30 2013-09-03 Illumina, Inc. Microvessels, microparticles, and methods of manufacturing and using the same
US20120245041A1 (en) 2009-11-04 2012-09-27 Sydney Brenner Base-by-base mutation screening
US9133343B2 (en) 2009-11-30 2015-09-15 Enzo Biochem, Inc. Dyes and compositions, and processes for using same in analysis of protein aggregation and other applications
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
WO2011087789A2 (en) 2009-12-22 2011-07-21 Becton, Dickinson And Company Methods for the detection of microorganisms
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
EP2524053A1 (en) * 2010-01-15 2012-11-21 Steffen Mergemeier Method for detecting more than one target in a pcr-based approach applying an unspecific dye which is not interfering with the emission of fluorophore-labeled probes
US20110177512A1 (en) * 2010-01-19 2011-07-21 Predictive Biosciences, Inc. Method for assuring amplification of an abnormal nucleic acid in a sample
WO2011101744A2 (en) 2010-02-22 2011-08-25 Population Genetics Technologies Ltd. Region of interest extraction and normalization methods
WO2011107887A2 (en) 2010-03-02 2011-09-09 Population Genetic Technologies Ltd. Methods for replicating polynucleotides with secondary structure
GB201004339D0 (en) 2010-03-16 2010-04-28 Enigma Diagnostics Ltd Sequence detection assay
EP2554680B1 (en) * 2010-03-29 2016-10-12 Toppan Printing Co., Ltd. Method for distinguishing target base sequence
DE102010003781B4 (de) * 2010-04-08 2012-08-16 Aj Innuscreen Gmbh Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen
US20110250598A1 (en) 2010-04-12 2011-10-13 Ulrike Fischer Detergent free polymerases
US8774494B2 (en) 2010-04-30 2014-07-08 Complete Genomics, Inc. Method and system for accurate alignment and registration of array for DNA sequencing
WO2011138402A1 (en) 2010-05-05 2011-11-10 Check-Points Holding B.V. Assays, compositions and methods for detecting drug resistant micro-organisms
SG10201503534RA (en) 2010-05-06 2015-06-29 Sequenta Inc Monitoring health and disease status using clonotype profiles
GB201007868D0 (en) 2010-05-11 2010-06-23 Enigma Diagnostics Ltd Sequence detection assay
GB201007867D0 (en) 2010-05-11 2010-06-23 Enigma Diagnostics Ltd Signalling system
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
EP3290529B1 (en) 2010-06-11 2019-05-22 Life Technologies Corporation Alternative nucleotide flows in sequencing-by-synthesis methods
EP2585593B1 (en) 2010-06-24 2017-03-01 Population Genetics Technologies Ltd. Methods for polynucleotide library production, immortalization and region of interest extraction
US9650629B2 (en) 2010-07-07 2017-05-16 Roche Molecular Systems, Inc. Clonal pre-amplification in emulsion
CA2802550C (en) 2010-07-29 2019-01-15 F. Hoffmann-La Roche Ag Generic pcr
EP2598655B1 (en) 2010-07-29 2016-10-05 F.Hoffmann-La Roche Ag Qualitative and quantitative detection of microbial nucleic acids
ES2617258T5 (es) 2010-07-29 2023-11-15 Hoffmann La Roche Preparación de muestras genéricas
CA2802567C (en) 2010-07-29 2017-12-05 F. Hoffmann La-Roche Ag Control nucleic acids for multiple parameters
EP2601286A4 (en) 2010-08-02 2016-10-05 Brent L Weight UNDER PRESSURE CARTRIDGES FOR POLYMERASE CHAIN REACTIONS
JP5959516B2 (ja) 2010-08-18 2016-08-02 ライフ テクノロジーズ コーポレーション 電気化学的検出装置のためのマイクロウェルの化学コーティング法
US9051606B2 (en) 2010-09-10 2015-06-09 Qiagen Gaithersburg, Inc. Methods and compositions for nucleic acid detection
NZ608153A (en) 2010-09-13 2014-12-24 Clinical Genomics Pty Ltd Epigenetic markers of colorectal cancers and diagnostic methods using the same
PT2623613T (pt) 2010-09-21 2016-10-11 Population Genetics Tech Ltd Aumento da confiança da designação de alelos por contagem molecular
WO2012038049A2 (en) 2010-09-22 2012-03-29 Roche Diagnostics Gmbh Amplification of distant nucleic acid targets using engineered primers
CN103124797B (zh) 2010-10-04 2015-04-22 霍夫曼-拉罗奇有限公司 用于在相同反应容器内的细胞裂解和pcr的方法
ES2533732T3 (es) 2010-10-04 2015-04-14 F. Hoffmann-La Roche Ag Método para lisis celular en un tampón de reacción de RT-PCR
CN103124796B (zh) 2010-10-04 2016-08-03 霍夫曼-拉罗奇有限公司 用于pcr反应缓冲液中的细胞裂解的方法
ES2730951T3 (es) 2010-10-08 2019-11-13 Harvard College Secuenciación inmune de alto rendimiento
GB201017011D0 (en) 2010-10-08 2010-11-24 Skova Services Ltd Assay
GB2512213B (en) 2010-10-08 2015-02-11 Harvard College High-throughput single cell barcoding
US8725422B2 (en) 2010-10-13 2014-05-13 Complete Genomics, Inc. Methods for estimating genome-wide copy number variations
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
KR101420094B1 (ko) 2010-10-27 2014-07-17 (주)바이오니아 다양한 생체시료분석을 위한 전자동실시간정량증폭장비, 다양한 생체시료분석을 위한 자동정제 및 반응준비 장치, 전자동 핵산정제 및 실시간 정량 유전자증폭 방법, 전자동 핵산정제방법, 실시간정량ｐｃｒ을 이용한 병원균의 전자동 생균수검사방법, 정량면역ｐｃｒ을 이용한 전자동 항원농도획득방법 및 타겟항원에 라벨링된 부착용 타겟핵산의 정제방법
JP6296536B2 (ja) 2010-11-05 2018-03-20 メドベット サイエンス ピーティーワイエルティーディーＭｅｄｖｅｔ Ｓｃｉｅｎｃｅ Ｐｔｙ Ｌｔｄ 内皮前駆細胞のマーカーおよびその使用
RU2451086C1 (ru) * 2010-12-03 2012-05-20 Государственное образовательное учреждение высшего профессионального образования Смоленская государственная медицинская академия федерального агентства по здравоохранению и социальному развитию Способ детекции специфических нуклеотидных последовательностей и нуклеотидных замен с помощью пцр в режиме реального времени с эффектом гашения флуоресценции зонда праймером
US8569254B2 (en) 2010-12-10 2013-10-29 National Yang Ming University Methods for modulating the expression and aggregation of CAG-expanded gene product in cells and methods for identifying agents useful for doing the same
EP2465945A1 (en) 2010-12-17 2012-06-20 F. Hoffmann-La Roche AG Generic matrix for control nucleic acids
WO2012087135A1 (en) 2010-12-22 2012-06-28 Academisch Ziekenhuis Leiden H.O.D.N. Lumc Genetic markers specific for clostridium difficile ribotypes 027 (nap01/b1; rt 027) and 078 (nap7/8; rt 078) and their use
EP2659408B1 (en) 2010-12-29 2019-03-27 Life Technologies Corporation Time-warped background signal for sequencing-by-synthesis operations
US10241075B2 (en) 2010-12-30 2019-03-26 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
EP3582224A1 (en) 2010-12-30 2019-12-18 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
US20130060482A1 (en) 2010-12-30 2013-03-07 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
EP2663652B1 (en) 2011-01-11 2018-11-14 Roche Diagniostics GmbH High resolution melting analysis as a prescreening tool
MX2017015093A (es) 2011-01-11 2023-03-10 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
US9096892B1 (en) 2011-02-04 2015-08-04 K2 Biomicrosystems, LLC Nucleic acid amplification and monitoring apparatus
WO2012108864A1 (en) 2011-02-08 2012-08-16 Illumina, Inc. Selective enrichment of nucleic acids
FR2971258B1 (fr) 2011-02-09 2020-12-04 Bio Rad Pasteur Combinaison de biomarqueurs pour le pronostic d'une reponse ou non-reponse a un traitement anti-vhc
FR2971257B1 (fr) 2011-02-09 2020-12-04 Bio Rad Pasteur Combinaison de biomarqueurs pour le pronostic d'une reponse ou non-reponse a un traitement anti-vhc
FR2971256B1 (fr) 2011-02-09 2024-09-27 Bio Rad Pasteur Combinaison de biomarqueurs pour la detection et l'evaluation d'une fibrose hepatique
PT3270141T (pt) 2011-03-08 2020-08-28 Univ Laval Dispositivo centrípeto fluídico
EP3878975A1 (en) 2011-04-08 2021-09-15 Life Technologies Corporation Phase-protecting reagent flow orderings for use in sequencing-by-synthesis
CN106190806B (zh) 2011-04-15 2018-11-06 贝克顿·迪金森公司 扫描实时微流体热循环仪和用于同步的热循环和扫描光学检测的方法
WO2012142656A1 (en) 2011-04-18 2012-10-26 Garvan Institute Of Medical Research Method of diagnosing cancer
KR102252447B1 (ko) * 2011-05-04 2021-05-17 바이오셉트 인코포레이티드 핵산 서열 변이체를 검출하는 방법
EP2707507B1 (en) 2011-05-09 2017-11-01 Fluidigm Corporation Probe based nucleic acid detection
EP2707496A1 (en) 2011-05-11 2014-03-19 Diagon Kft. Procedure for rapid determination of viruses using nucleic acid-based molecular diagnostics, and a kit for this purpose
US8663919B2 (en) 2011-05-18 2014-03-04 Life Technologies Corporation Chromosome conformation analysis
DK2714939T3 (en) 2011-05-24 2015-06-29 Elitech Holding Bv DETECTION OF METHICILLIN-RESISTENT STAPHYLOCOCCUS AUREUS
US9034581B2 (en) 2011-05-26 2015-05-19 Roche Molecular Systems, Inc. Compositions and methods for detection of Staphylococcus aureus
CA2835654A1 (en) * 2011-06-01 2012-12-06 Streck, Inc. Rapid thermocycler system for rapid amplification of nucleic acids and related methods
WO2012171997A1 (en) 2011-06-14 2012-12-20 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for determining the expression level of a gene of interest including correction of rt-qpcr data for genomic dna-derived signals
EP4324935B1 (en) 2011-08-18 2025-02-19 Life Technologies Corporation Methods, systems and computer readable media for making base calls in nucleic acid sequencing
EP2744912B1 (en) 2011-08-18 2016-11-02 Nestec S.A. Compositions and methods for detecting allelic variants
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
US20150087537A1 (en) 2011-08-31 2015-03-26 Life Technologies Corporation Methods, Systems, Computer Readable Media, and Kits for Sample Identification
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
WO2013040251A2 (en) 2011-09-13 2013-03-21 Asurgen, Inc. Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
WO2013040491A2 (en) 2011-09-15 2013-03-21 Shafer David A Probe: antiprobe compositions for high specificity dna or rna detection
DK2756098T3 (en) 2011-09-16 2018-09-03 Lexogen Gmbh Process for Preparing a Library of Nucleic Acid Molecules
EP2570487A1 (en) 2011-09-16 2013-03-20 Lexogen GmbH Nucleic acid transcription method
CN107267615A (zh) 2011-09-23 2017-10-20 霍夫曼-拉罗奇有限公司 G形夹用于改进的等位基因特异性pcr的用途
EP3964285A1 (en) 2011-09-26 2022-03-09 Thermo Fisher Scientific Geneart GmbH High efficiency, small volume nucleic acid synthesis
WO2013049613A1 (en) 2011-09-29 2013-04-04 Luminex Corporation Hydrolysis probes
US9416153B2 (en) 2011-10-11 2016-08-16 Enzo Life Sciences, Inc. Fluorescent dyes
ES2660228T3 (es) 2011-10-14 2018-03-21 Becton Dickinson & Company Ciclado térmico de onda cuadrada
US10501791B2 (en) 2011-10-14 2019-12-10 President And Fellows Of Harvard College Sequencing by structure assembly
US9279159B2 (en) 2011-10-21 2016-03-08 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
CN104040238B (zh) 2011-11-04 2017-06-27 汉迪拉布公司 多核苷酸样品制备装置
US9447458B2 (en) 2011-11-16 2016-09-20 Canon U.S. Life Sciences, Inc. Detection of neighboring variants
US9260714B2 (en) 2011-12-02 2016-02-16 Roche Molecular Systems, Inc. Suppression of non-specific amplification with high-homology oligonucleotides
EP2788509B1 (en) 2011-12-09 2018-07-11 Adaptive Biotechnologies Corporation Diagnosis of lymphoid malignancies and minimal residual disease detection
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
WO2013093432A1 (en) 2011-12-19 2013-06-27 David Kelly Method for enriching and detection of variant target nucleic acids
US8981318B1 (en) 2011-12-30 2015-03-17 Gene Capture, Inc. Multi-dimensional scanner for nano-second time scale signal detection
US9194840B2 (en) 2012-01-19 2015-11-24 Life Technologies Corporation Sensor arrays and methods for making same
WO2013112458A1 (en) 2012-01-24 2013-08-01 Beth Israel Deaconess Medical Center, Inc. Novel chrebp isoforms and methods using the same
US9864846B2 (en) 2012-01-31 2018-01-09 Life Technologies Corporation Methods and computer program products for compression of sequencing data
US9515676B2 (en) 2012-01-31 2016-12-06 Life Technologies Corporation Methods and computer program products for compression of sequencing data
WO2013113748A1 (en) * 2012-02-02 2013-08-08 Primer Design Ltd Method for detecting and genotyping target nucleic acid
JP6255353B2 (ja) 2012-02-03 2017-12-27 カリフォルニア インスティチュート オブ テクノロジー 多重生化学アッセイにおける信号エンコードおよびデコード
US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
WO2013124743A1 (en) 2012-02-22 2013-08-29 Population Genetics Technologies Ltd. Compositions and methods for intramolecular nucleic acid rearrangement ii
EP2820155B1 (en) 2012-02-28 2017-07-26 Population Genetics Technologies Ltd. Method for attaching a counter sequence to a nucleic acid sample
ES2662128T3 (es) 2012-03-05 2018-04-05 Adaptive Biotechnologies Corporation Determinación de cadenas de receptor inmunitario emparejadas a partir de la frecuencia de subunidades coincidentes
JP5976849B2 (ja) 2012-03-05 2016-08-24 シージーン アイエヌシー ＰＴＯ切断及び伸長アッセイによるターゲット核酸配列でのヌクレオチド変異検出｛ＤｅｔｅｃｔｉｏｎｏｆＮｕｃｌｅｏｔｉｄｅＶａｒｉａｔｉｏｎｏｎＴａｒｇｅｔＮｕｃｌｅｉｃＡｃｉｄＳｅｑｕｅｎｃｅｂｙＰＴＯＣｌｅａｖａｇｅａｎｄＥｘｔｅｎｓｉｏｎＡｓｓａｙ｝
WO2013139860A1 (en) 2012-03-21 2013-09-26 Roche Diagnostics Gmbh Methods for assessing rna quality
US9803239B2 (en) 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
MX354329B (es) 2012-05-08 2018-02-27 Adaptive Biotechnologies Corp Composiciones y metodos para medir y calibrar el error de amplificacion en reacciones de pcr multiple.
CN104411835B (zh) 2012-05-11 2019-12-20 临床基因组学股份有限公司 用于结直肠癌的诊断性基因标记系列
US9646132B2 (en) 2012-05-11 2017-05-09 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
DK2850212T3 (da) 2012-05-18 2020-03-23 Clinical Genomics Pty Ltd Fremgangsmåde til screening for colorektal cancer
EP3553181A1 (en) * 2012-05-25 2019-10-16 Accugenomics, Inc. Nucleic acid amplification and use thereof
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
US9488823B2 (en) 2012-06-07 2016-11-08 Complete Genomics, Inc. Techniques for scanned illumination
US9628676B2 (en) 2012-06-07 2017-04-18 Complete Genomics, Inc. Imaging systems with movable scan mirrors
WO2013188839A1 (en) 2012-06-14 2013-12-19 Life Technologies Corporation Novel compositions, methods and kits for real time polymerase chain reaction (pcr)
KR101743846B1 (ko) 2012-07-19 2017-06-05 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산을 이용하여 정보를 저장하는 방법
US9932628B2 (en) 2012-07-27 2018-04-03 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
EP2880184B1 (en) 2012-08-03 2021-03-31 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
US9957557B2 (en) 2012-08-13 2018-05-01 Life Genetics Lab, Llc Development of a highly sensitive quantification system for assessing DNA degradation and quality in forensic samples
US10993418B2 (en) 2012-08-13 2021-05-04 Life Genetics Lab, Llc Method for measuring tumor burden in patient derived xenograft (PDX) mice
US9982313B2 (en) 2012-08-17 2018-05-29 Roche Molecular Systems, Inc. Compositions and methods for detection of herpes simplex virus 1 and 2
CA2880583C (en) 2012-09-10 2021-02-16 Randy P. Rasmussen Multiple amplification cycle detection
US9914968B2 (en) 2012-09-26 2018-03-13 Cepheid Honeycomb tube
US20160002731A1 (en) 2012-10-01 2016-01-07 Adaptive Biotechnologies Corporation Immunocompetence assessment by adaptive immune receptor diversity and clonality characterization
US10176293B2 (en) 2012-10-02 2019-01-08 Roche Molecular Systems, Inc. Universal method to determine real-time PCR cycle threshold values
EP2906943B1 (en) * 2012-10-09 2018-07-25 University Of Utah Research Foundation Monitoring temperature with fluorescence
US10329608B2 (en) 2012-10-10 2019-06-25 Life Technologies Corporation Methods, systems, and computer readable media for repeat sequencing
US9476089B2 (en) 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
EP2722399A1 (en) 2012-10-18 2014-04-23 Roche Diagniostics GmbH Method for preventing high molecular weight products during amplification
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
CN103114131B (zh) 2012-11-30 2018-10-02 珠海市坤元农业科技有限公司 一种引物中部序列干扰pcr技术
CN105121661B (zh) 2013-02-01 2018-06-08 加利福尼亚大学董事会 用于基因组组装及单体型定相的方法
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
ES2662825T3 (es) 2013-02-25 2018-04-09 Seegene, Inc. Detección de variación de nucleótido en una secuencia de ácidos nucleicos diana
WO2014142575A1 (en) 2013-03-13 2014-09-18 Seegene, Inc. Quantification of target nucleic acid using melting peak analysis
AU2013202788B2 (en) 2013-03-14 2015-10-01 Gen-Probe Incorporated Indexing signal detection module
US20140296080A1 (en) 2013-03-14 2014-10-02 Life Technologies Corporation Methods, Systems, and Computer Readable Media for Evaluating Variant Likelihood
AU2013202805B2 (en) 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
US10563240B2 (en) 2013-03-14 2020-02-18 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
CN105745546B (zh) 2013-03-15 2017-10-13 雅培制药有限公司 具有后面可进入轨道系统的自动化诊断分析仪及相关方法
US20140273181A1 (en) 2013-03-15 2014-09-18 Biofire Diagnostics, Inc. Compact optical system for substantially simultaneous monitoring of samples in a sample array
JP6412101B2 (ja) 2013-03-15 2018-10-24 アベリノ ラボ ユーエスエー インコーポレイテッドＡｖｅｌｌｉｎｏ Ｌａｂ ＵＳＡ， Ｉｎｃ． アレル検出用ゲノムｄｎａテンプレートの改良された単離方法
US9816088B2 (en) 2013-03-15 2017-11-14 Abvitro Llc Single cell bar-coding for antibody discovery
US10933417B2 (en) 2013-03-15 2021-03-02 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
JP6351703B2 (ja) 2013-03-15 2018-07-04 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ 垂直配置カルーセルを有する自動診断分析装置および関連方法
CN105190317B (zh) 2013-03-15 2018-05-04 雅培制药有限公司 具有预处理转盘的诊断分析机及相关方法
US9708658B2 (en) 2013-03-19 2017-07-18 New England Biolabs, Inc. Enrichment of target sequences
US20160085908A1 (en) 2013-04-23 2016-03-24 Thermo Fisher Scientific Oy Melt Curve Analysis
EP2999796B1 (en) 2013-05-20 2017-10-25 BioVentures, LLC Gep5 model for multiple myeloma
EP3521452B1 (en) 2013-06-19 2021-08-04 Luminex Corporation Real-time multiplexed hydrolysis probe assay
CN105378111B (zh) * 2013-06-25 2020-03-20 C·威特沃 进行聚合酶链式反应的方法及其相关应用
WO2014210593A1 (en) 2013-06-28 2014-12-31 Streck, Inc. Devices for real-time polymerase chain reaction
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
WO2015008985A1 (en) 2013-07-15 2015-01-22 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
US9926597B2 (en) 2013-07-26 2018-03-27 Life Technologies Corporation Control nucleic acid sequences for use in sequencing-by-synthesis and methods for designing the same
EP3030680B1 (en) 2013-08-09 2018-10-03 Luminex Corporation Melt discrimination and multiplexing in nucleic acid assays
US20160186265A1 (en) 2013-08-15 2016-06-30 Centre Hospitalier Universitarie Vaudois Methods for Typing HLA Alleles
US10410739B2 (en) 2013-10-04 2019-09-10 Life Technologies Corporation Methods and systems for modeling phasing effects in sequencing using termination chemistry
US10370707B2 (en) 2013-10-09 2019-08-06 Fluoresentric, Inc. Multiplex probes
US10072288B2 (en) 2013-11-11 2018-09-11 Roche Molecular Systems, Inc. Detecting single nucleotide polymorphism using overlapped primer and melting probe
KR102483783B1 (ko) 2013-11-15 2022-12-30 아벨리노 랩 유에스에이, 인크. 안과 질환과 관련된 대립유전자의 멀티플렉스 검출 방법
US10927408B2 (en) 2013-12-02 2021-02-23 Personal Genome Diagnostics, Inc. Method for evaluating minority variants in a sample
US9476853B2 (en) 2013-12-10 2016-10-25 Life Technologies Corporation System and method for forming microwells
EP3080605B1 (en) 2013-12-11 2019-02-20 The Regents of the University of California Method for labeling dna fragments to reconstruct physical linkage and phase
AU2015218821B2 (en) 2014-02-21 2019-01-24 President And Fellows Of Harvard College De novo design of allosteric proteins
CA2941612A1 (en) 2014-03-05 2015-09-11 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
WO2015138343A1 (en) 2014-03-10 2015-09-17 Click Diagnostics, Inc. Cartridge-based thermocycler
EP2921556A1 (en) 2014-03-21 2015-09-23 Lexogen GmbH Copy number preserving RNA analysis method
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
WO2015154028A1 (en) 2014-04-04 2015-10-08 Affymetrix, Inc. Improved compositions and methods for molecular inversion probe assays
BE1022048B1 (nl) * 2014-04-11 2016-02-10 Ugentec Bvba Methoden voor fluorescentie data correctie
US10124333B2 (en) 2014-06-10 2018-11-13 Reolab Inc. Discrete elements for 3D microfluidics
DE102014212657B4 (de) * 2014-06-30 2016-03-10 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. System und Verfahren zur bedarfsgerechten Zuführung von Beleuchtungsenergie an Pflanzen
GB201411567D0 (en) * 2014-06-30 2014-08-13 Epistem Ltd Quantification methods
WO2016019360A1 (en) 2014-08-01 2016-02-04 Dovetail Genomics Llc Tagging nucleic acids for sequence assembly
DE102014215352A1 (de) * 2014-08-04 2016-02-04 Berthold Technologies Gmbh & Co. Kg Messgerät und Verfahren zum Bestimmen einer Messgröße
EP3180449B1 (en) 2014-08-11 2019-10-09 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
AU2015318823A1 (en) 2014-09-15 2016-03-24 Garvan Institute Of Medical Research Methods for diagnosis, prognosis and monitoring of breast cancer and reagents therefor
NZ730044A (en) 2014-09-15 2024-08-30 Abvitro Llc High-throughput nucleotide library sequencing
HUE055583T2 (hu) * 2014-09-16 2021-12-28 Sangamo Therapeutics Inc Eljárások és készítmények nukleáz által közvetített genommódosításhoz és -javításhoz hematopoetikus õssejtekben
CN107002138B (zh) 2014-09-30 2022-06-14 基因技术有限公司 用于评估发展乳腺癌风险的方法
WO2016060974A1 (en) 2014-10-13 2016-04-21 Life Technologies Corporation Methods, systems, and computer-readable media for accelerated base calling
ES2784343T3 (es) 2014-10-29 2020-09-24 Adaptive Biotechnologies Corp Detección simultánea altamente multiplexada de ácidos nucleicos que codifican heterodímeros de receptores inmunes adaptativos emparejados de muchas muestras
CA2967036A1 (en) 2014-11-13 2016-05-19 The Board Of Trustees Of The University Of Illinois Bio-engineered hyper-functional "super" helicases
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
US9745618B2 (en) 2014-11-19 2017-08-29 Roche Molecular Systems, Inc. Photoblocked probes and methods for sequential detection of nucleic acids
EP3498866A1 (en) 2014-11-25 2019-06-19 Adaptive Biotechnologies Corp. Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
US9920381B2 (en) 2014-12-02 2018-03-20 Roche Molecular Systems, Inc. Compositions and methods for detecting MECC-containing methicillin-resistant Staphylococcus aureus
CA2970477C (en) 2014-12-09 2022-03-15 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
WO2016093838A1 (en) 2014-12-11 2016-06-16 New England Biolabs, Inc. Enrichment of target sequences
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
MX379999B (es) 2014-12-31 2025-03-11 Visby Medical Inc Dispositivos y métodos para prueba de diagnóstico molecular
CN107250381B (zh) 2015-01-16 2022-03-08 斯库维尔公司 Dna集合的归一化迭代条形码和测序
US10421993B2 (en) 2015-02-11 2019-09-24 Paragon Genomics, Inc. Methods and compositions for reducing non-specific amplification products
KR102721363B1 (ko) 2015-02-17 2024-10-23 더브테일 제노믹스 엘엘씨 핵산 서열 어셈블리
AU2016222788B2 (en) 2015-02-24 2022-03-31 Adaptive Biotechnologies Corp. Methods for diagnosing infectious disease and determining HLA status using immune repertoire sequencing
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US11807896B2 (en) 2015-03-26 2023-11-07 Dovetail Genomics, Llc Physical linkage preservation in DNA storage
US9957393B2 (en) 2015-03-30 2018-05-01 Enzo Biochem, Inc. Monoazo dyes with cyclic amine as fluorescence quenchers
EP3277294B1 (en) 2015-04-01 2024-05-15 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
CN114250274A (zh) 2015-04-24 2022-03-29 阿提拉生物系统公司 利用有限核苷酸组成的引物扩增
US10968475B2 (en) 2015-05-01 2021-04-06 Vanderbilt University Monitoring and analysis of nucleic acid hybridization state and amplification using L-DNA
CN107969138B (zh) 2015-05-14 2022-04-12 生命科技公司 条形码序列和有关系统与方法
US20180155764A1 (en) 2015-06-04 2018-06-07 Mizuho Medy Co., Ltd. Kit for together detecting multiple target nucleic acids differing from each other and detection method using the same
CN107849726B (zh) * 2015-06-08 2021-07-13 芝加哥大学 单分散贵金属纳米晶体的双锥体模板化合成
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
IL258248B (en) 2015-09-24 2022-07-01 Abvitro Llc Attraction Oligonucleotide Conjugates and Their Uses
JP7341661B2 (ja) 2015-09-25 2023-09-11 アブビトロ， エルエルシー ネイティブに対合するｔ細胞受容体配列のｔ細胞受容体標的識別のための高スループットプロセス
US11266730B2 (en) 2015-09-29 2022-03-08 The General Hospital Corporation Methods of treating and diagnosing disease using biomarkers for BCG therapy
CN108368542B (zh) 2015-10-19 2022-04-08 多弗泰尔基因组学有限责任公司 用于基因组组装、单元型定相以及独立于靶标的核酸检测的方法
USD799715S1 (en) 2015-10-23 2017-10-10 Gene POC, Inc. Fluidic centripetal device
US10808287B2 (en) 2015-10-23 2020-10-20 Rapid Pathogen Screening, Inc. Methods and devices for accurate diagnosis of infections
WO2017083852A1 (en) 2015-11-13 2017-05-18 MOORE, Tara Methods for the treatment of corneal dystrophies
EP3387107B1 (en) 2015-12-11 2020-08-12 Spartan Bioscience Inc. Tube sealing system and methods for nucleic acid amplification
SG10202111007PA (en) 2015-12-18 2021-11-29 Biofire Defense Llc Solid fluorescence standard
AU2017212152B2 (en) 2016-01-28 2019-07-11 The University Of Melbourne Methods for assessing risk of developing colorectal cancer
WO2017147279A1 (en) 2016-02-23 2017-08-31 Dovetail Genomics Llc Generation of phased read-sets for genome assembly and haplotype phasing
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
BR112018068139B1 (pt) 2016-03-10 2022-12-06 Pioneer Hi-Bred International, Inc Sistema de detecção de produto e amplificação de reação em cadeia da polimerase (pcr) e método de detecção de produto e amplificação de pcr mediada por luz
JP6962927B2 (ja) 2016-03-11 2021-11-05 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft ジカウイルス検出用組成物および方法
WO2017185067A1 (en) 2016-04-22 2017-10-26 Click Diagnostics, Inc. Printed circuit board heater for an amplification module
US10619205B2 (en) 2016-05-06 2020-04-14 Life Technologies Corporation Combinatorial barcode sequences, and related systems and methods
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
CN109477101B (zh) 2016-05-13 2022-11-18 多弗泰尔基因组学有限责任公司 从保存的样品中回收长范围连锁信息
WO2017201315A1 (en) 2016-05-18 2017-11-23 Roche Sequencing Solutions, Inc. Quantitative real time pcr amplification using an electrowetting-based device
US10612101B2 (en) 2016-05-27 2020-04-07 Roche Molecular Systems, Inc. Compositions and methods for detection of Mycoplasma genitalium
JP7098536B2 (ja) 2016-05-27 2022-07-11 エフ．ホフマン－ラ ロシュ アーゲー 膣トリコモナスの検出のための組成物及び方法
CN109643578B (zh) 2016-06-01 2023-07-21 生命科技股份有限公司 用于设计基因组合的方法和系统
US20170356025A1 (en) 2016-06-14 2017-12-14 Roche Molecular Systems, Inc. Internal control probes for improving pcr assay performance
US10066263B2 (en) * 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
RU2691763C2 (ru) * 2016-06-21 2019-06-18 Федеральное государственное бюджетное учреждение науки Институт аналитического приборостроения Российской академии наук (ИАП РАН) Устройство для одновременного контроля в реальном масштабе времени множества амплификаций нуклеиновой кислоты
CN110325652A (zh) 2016-06-29 2019-10-11 易捷仪器诊断股份有限公司 使用流动池检测分子的装置和方法
US10626454B2 (en) 2016-07-27 2020-04-21 SeqMatic, LLC Compositions and methods for nucleic acid amplification and analysis
CN109804089A (zh) 2016-07-29 2019-05-24 朱诺治疗学股份有限公司 用于评估复制型病毒存在或不存在的方法
JP6999645B2 (ja) 2016-08-02 2022-02-04 エフ．ホフマン－ラ ロシュ アーゲー 核酸の増幅及び検出／定量の効率を改良するためのヘルパーオリゴヌクレオチド
KR102468174B1 (ko) 2016-09-15 2022-11-17 에프. 호프만-라 로슈 아게 멀티플렉스 pcr 수행 방법
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
WO2018057928A1 (en) 2016-09-23 2018-03-29 Grail, Inc. Methods of preparing and analyzing cell-free nucleic acid sequencing libraries
AU2017332495A1 (en) 2016-09-24 2019-04-11 Abvitro Llc Affinity-oligonucleotide conjugates and uses thereof
US9851345B1 (en) 2016-10-12 2017-12-26 Viasphere, Llc Compositions and methods for disease diagnosis using single cell analysis
DE112016007245T5 (de) 2016-10-17 2019-08-14 Olympus Corporation Verfahren zum Nachweisen von Ziel-Nukleinsäuremolekülen
WO2018085862A2 (en) 2016-11-07 2018-05-11 Grail, Inc. Methods of identifying somatic mutational signatures for early cancer detection
US10793923B2 (en) 2016-11-09 2020-10-06 Roche Molecular Systems, Inc. Compositions and methods for detection of BK virus
EP3551768B1 (en) 2016-12-12 2024-03-06 Grail, LLC Methods for tagging and amplifying rna template molecules for preparing sequencing libraries
US20180163270A1 (en) 2016-12-12 2018-06-14 Cepheid Integrated immuno-pcr and nucleic acid analysis in an automated reaction cartridge
US20190211377A1 (en) 2016-12-22 2019-07-11 Roche Molecular Systems, Inc. Cobra probes to detect a marker for epidemic ribotypes of clostridium difficile
EP3559255A1 (en) 2016-12-23 2019-10-30 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
US10793901B2 (en) 2016-12-28 2020-10-06 Roche Molecular Systems, Inc. Reversibly protected nucleotide reagents with high thermal stability
ES3026859T3 (en) 2017-01-04 2025-06-12 Mgi Tech Co Ltd Stepwise sequencing by non-labeled reversible terminators or natural nucleotides
US10100358B2 (en) 2017-01-10 2018-10-16 Paragon Genomics, Inc. Methods and compositions for reducing redundant molecular barcodes created in primer extension reactions
CA3176529C (en) 2017-03-24 2024-03-05 Gen-Probe Incorporated Compositions and methods for detection of viral pathogens in samples
US10328147B2 (en) 2017-03-24 2019-06-25 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Herpes simplex virus type-1(HSV-1) vaccine strain VC2 generating an anti-EHV-1 immune response
US11274344B2 (en) 2017-03-30 2022-03-15 Grail, Inc. Enhanced ligation in sequencing library preparation
WO2018183897A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Higher target capture efficiency using probe extension
US11584958B2 (en) 2017-03-31 2023-02-21 Grail, Llc Library preparation and use thereof for sequencing based error correction and/or variant identification
AU2018258580A1 (en) 2017-04-28 2019-11-21 Avellino Lab Usa, Inc. Methods for detecting alleles associated with keratoconus
WO2018213803A1 (en) 2017-05-19 2018-11-22 Neon Therapeutics, Inc. Immunogenic neoantigen identification
US20190093155A1 (en) 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
CA3064205A1 (en) 2017-05-26 2018-11-29 Abvitro Llc High-throughput polynucleotide library sequencing and transcriptome analysis
US20180363044A1 (en) 2017-06-14 2018-12-20 Roche Molecular Systems, Inc. Compositions and methods for improving the thermal stability of nucleic acid amplification reagents
US11450121B2 (en) 2017-06-27 2022-09-20 The Regents Of The University Of California Label-free digital brightfield analysis of nucleic acid amplification
CN111050545A (zh) 2017-06-29 2020-04-21 朱诺治疗学股份有限公司 评估与免疫疗法相关的毒性的小鼠模型
EP4286055B1 (en) 2017-07-10 2025-03-19 Gen-Probe Incorporated Method for determining the presence of multiple forms of a nucleic acid analyte in a sample
US10760137B2 (en) 2017-07-18 2020-09-01 Roche Molecular Systems, Inc. Compositions and methods for detection of Babesia
KR20200064060A (ko) 2017-07-29 2020-06-05 주노 쎄러퓨티크스 인코퍼레이티드 재조합 수용체를 발현하는 세포 증폭용 시약
WO2019045532A2 (en) 2017-08-31 2019-03-07 Seegene, Inc. EVALUATION OF COMPONENT PERFORMANCE USING A PAIR OF DIMER FORMER PRIMERS
MA50057A (fr) 2017-09-01 2020-07-08 Juno Therapeutics Inc Expression génique et évaluation d'un risque de développement d'une toxicité suite à une thérapie cellulaire
WO2019055819A1 (en) 2017-09-14 2019-03-21 Grail, Inc. METHODS FOR PREPARING A SEQUENCING LIBRARY FROM SINGLE STRANDED DNA
EP4269583B1 (en) 2017-09-28 2025-12-31 Grail, Inc. ENRICHEMENT OF SHORT NUCLEIC ACID FRAGMENTS IN SEQUENCING BANK PREPARATION
WO2019063661A1 (en) 2017-09-29 2019-04-04 Roche Diagnostics Gmbh COMPOSITIONS AND METHODS FOR THE DETECTION OF TRICHOMONAS VAGINALIS
US11401546B2 (en) 2017-09-29 2022-08-02 Seegene, Inc. Detection of target nucleic acid sequences by PTO cleavage and extension-dependent extension assay
US11732257B2 (en) 2017-10-23 2023-08-22 Massachusetts Institute Of Technology Single cell sequencing libraries of genomic transcript regions of interest in proximity to barcodes, and genotyping of said libraries
US20210254000A1 (en) 2017-11-01 2021-08-19 Juno Therapeutics, Inc. Process for producing a t cell composition
JP7239568B2 (ja) 2017-11-09 2023-03-14 ビスビュー メディカル，インコーポレイテッド 携帯型分子診断デバイスおよび標的ウイルスの検出方法
US11078534B2 (en) 2017-11-27 2021-08-03 Roche Molecular Systems, Inc. Photocleavable nucleotide reagents with high stability
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
WO2019118925A1 (en) 2017-12-15 2019-06-20 Grail, Inc. Methods for enriching for duplex reads in sequencing and error correction
WO2019126803A1 (en) 2017-12-22 2019-06-27 Grail, Inc. Error removal using improved library preparation methods
WO2019135567A1 (en) 2018-01-05 2019-07-11 Seegene, Inc. . Method for determining the presence or absence of m. tuberculosis, m. bovis and m. bovis bcg in a sample
DK3746225T3 (da) 2018-01-29 2025-01-13 Gen Probe Inc Analytiske systemer og fremgangsmåder
EP3746566A1 (en) 2018-01-31 2020-12-09 Dovetail Genomics, LLC Sample prep for dna linkage recovery
CN111918972A (zh) 2018-01-31 2020-11-10 朱诺治疗学股份有限公司 评估存在或不存在有复制能力的病毒的方法和试剂
US20190292596A1 (en) 2018-03-21 2019-09-26 Roche Molecular Systems, Inc. Modified nucleoside phosphates with high thermal stability
EP3781710A4 (en) 2018-04-17 2022-01-05 Chromacode, Inc. METHODS AND SYSTEMS FOR MULTIPLEX ANALYSIS
US11384376B2 (en) 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
JP7470095B2 (ja) 2018-07-10 2024-04-17 ジェン－プローブ・インコーポレーテッド 核酸を検出および定量するための方法およびシステム
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
EP3836967A4 (en) 2018-07-30 2022-06-15 ReadCoor, LLC METHODS AND SYSTEMS FOR SAMPLE PROCESSING OR ANALYSIS
US20210324461A1 (en) 2018-08-16 2021-10-21 Life Technologies Corporation Reagents, mixtures, kits and methods for amplification of nucleic acids
EP3846940A4 (en) 2018-09-03 2022-07-27 Visby Medical, Inc. DEVICES AND METHODS FOR TESTING ANTIBIOTIC SUSCEPTIBILITY
JP7221491B2 (ja) * 2018-09-18 2023-02-14 株式会社ミズホメディー 複数の標的核酸を検出するキットを用いる検出方法
US20220195515A1 (en) 2018-10-29 2022-06-23 Koninklijke Nederlandse Akademie Van Wetenschappen Single cell full length RNA sequencing
CN113272018A (zh) 2018-11-06 2021-08-17 朱诺治疗学股份有限公司 产生基因工程化t细胞的方法
CN113166802A (zh) 2018-12-03 2021-07-23 豪夫迈·罗氏有限公司 用于检测耳道假丝酵母菌的组合物和方法
WO2020120442A2 (en) 2018-12-13 2020-06-18 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
CN113286883A (zh) 2018-12-18 2021-08-20 格里尔公司 使用rna分析以检测疾病的方法
CN113272442B (zh) 2019-01-03 2024-03-15 Dna斯克瑞普特公司 寡核苷酸的集合的一锅合成
CN119510370A (zh) 2019-03-14 2025-02-25 因斯利克萨公司 基于时间门控的荧光检测的方法和系统
EP4438054A3 (en) 2019-04-17 2025-01-08 Alpine Immune Sciences, Inc. Methods and uses of variant icos ligand (icosl) fusion proteins
WO2020223257A1 (en) 2019-04-28 2020-11-05 Visby Medical, Inc. Molecular diagnostic devices with digital detection capability and wireless connectivity
CN116786186A (zh) * 2019-05-01 2023-09-22 卢米耐克斯公司 用于热循环的装置以及样品模块
WO2020221915A1 (en) 2019-05-02 2020-11-05 F. Hoffmann-La Roche Ag UTILIZATION OF dITP FOR PREFERENTIAL/SELECTIVE AMPLIFICATION OF RNA VERSUS DNA TARGETS BASED ON STRAND-SEPARATION TEMPERATURE
WO2020225231A1 (en) 2019-05-07 2020-11-12 F. Hoffmann-La Roche Ag Compositions and methods for detection of neisseria gonorroheae
KR20220034782A (ko) 2019-06-12 2022-03-18 주노 쎄러퓨티크스 인코퍼레이티드 세포 매개 세포 독성 요법 및 친생존 ｂｃｌ2 패밀리 단백질 억제제의 병용 요법
CA3145212A1 (en) 2019-06-27 2020-12-30 Dovetail Genomics, Llc Methods and compositions for proximity ligation
CN114174540A (zh) 2019-07-25 2022-03-11 豪夫迈·罗氏有限公司 用于检测爱泼斯坦巴尔病毒(ebv)的组合物和方法
KR20220066892A (ko) 2019-08-22 2022-05-24 주노 쎄러퓨티크스 인코퍼레이티드 Ｔ 세포 요법 및 제스트 동족체 2의 인핸서 (ｅｚｈ2) 억제제의 병용 요법 및 관련 방법
CN114286866A (zh) 2019-08-27 2022-04-05 豪夫迈·罗氏有限公司 用于扩增和检测包括从cccDNA转录的HBV RNA在内的乙型肝炎病毒RNA的组合物和方法
US11441167B2 (en) 2019-11-20 2022-09-13 Roche Molecular Systems, Inc. Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi
US20230050863A1 (en) 2019-12-09 2023-02-16 Roche Molecular Systems, Inc. Dicationic fluorescent dyes
EP4081666B1 (en) 2019-12-27 2024-08-21 F. Hoffmann-La Roche AG Compositions and methods for detecting methicillin-resistant staphylococcus aureus
WO2021138544A1 (en) 2020-01-03 2021-07-08 Visby Medical, Inc. Devices and methods for antibiotic susceptibility testing
WO2021140173A1 (en) 2020-01-10 2021-07-15 Biouniversa S.R.L. Methods and uses for treating fibrotic solid tumors with bags inhibitors
CN115335511A (zh) 2020-01-13 2022-11-11 艾斯本神经科学公司 使神经细胞分化的方法及相关组合物和使用方法
EP4118239A1 (en) 2020-03-09 2023-01-18 F. Hoffmann-La Roche AG Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (sars-cov-2), influenza a and influenza b
US20210332448A1 (en) 2020-03-09 2021-10-28 Janssen Pharmaceutica Nv Compositions and methods for quantifying integration of recombinant vector nucleic acid
US20230129228A1 (en) 2020-03-18 2023-04-27 Mgi Tech Co. Ltd. Restoring phase in massively parallel sequencing
CN115835873A (zh) 2020-05-13 2023-03-21 朱诺治疗学股份有限公司 用于产生表达重组受体的供体分批细胞的方法
US10941453B1 (en) 2020-05-20 2021-03-09 Paragon Genomics, Inc. High throughput detection of pathogen RNA in clinical specimens
CA3179313A1 (en) 2020-05-27 2021-12-02 Gillian Sue Dite Methods of assessing risk of developing a severe response to coronavirus infection
CN116234558A (zh) 2020-06-26 2023-06-06 朱诺治疗学有限公司 条件性地表达重组受体的工程化t细胞、相关多核苷酸和方法
CN115885157A (zh) * 2020-07-20 2023-03-31 光热光谱股份有限公司 荧光增强的光热红外光谱学和共聚焦荧光成像
US20240052391A1 (en) 2020-10-29 2024-02-15 Dna Script Enzymatic Synthesis of Polynucleotide Probes
CN112461806B (zh) * 2020-11-18 2022-02-11 厦门大学 基于智能手机的荧光光谱检测方法
JP2023552546A (ja) 2020-12-04 2023-12-18 エフ． ホフマン－ラ ロシュ アーゲー マラリアを検出するための組成物および方法
JP7209980B2 (ja) 2020-12-11 2023-01-23 東洋紡株式会社 Ｄｎａポリメラーゼの５’→３’エキソヌクレアーゼ活性ドメインに特異的に結合する抗体
JP2024500169A (ja) 2020-12-22 2024-01-04 エフ． ホフマン－ラ ロシュ アーゲー 大ストークスシフト蛍光色素を使用して多重化リアルタイムｐｃｒを行うための方法
US20220205020A1 (en) 2020-12-30 2022-06-30 Roche Molecular Systems, Inc. Compositions and methods for detection of bacteria and fungi associated with bacterial and candida vaginosis
CA3203805A1 (en) 2021-01-08 2022-07-14 Cellanome, Inc. Devices and methods for analyzing biological samples
JP2024505686A (ja) 2021-02-05 2024-02-07 エフ． ホフマン－ラ ロシュ アーゲー ヒトパラインフルエンザウイルス１－４（ｈｐｉｖ１－４）の検出のための組成物及び方法
US20220290221A1 (en) 2021-03-15 2022-09-15 Roche Molecular Systems, Inc. Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (sars-cov-2) variants having spike protein mutations
JP2024517835A (ja) 2021-05-06 2024-04-23 エフ． ホフマン－ラ ロシュ アーゲー 二重標的アッセイによりデルタ型肝炎ウイルスを検出するための組成物及び方法
WO2023283452A2 (en) 2021-07-09 2023-01-12 Cepheid High-level multiplexing reaction vessel, reagent spotting device and associated methods
WO2023004371A1 (en) 2021-07-21 2023-01-26 Aspen Neuroscience, Inc. Methods of differentiating neural cells and predicting engraftment thereof and related compositions
KR20240058112A (ko) 2021-09-17 2024-05-03 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출
JP7641445B2 (ja) * 2021-09-30 2025-03-06 ジェン－プローブ・インコーポレーテッド 温度で選択可能なｆｒｅｔカセットシグナル伝達
US20230121442A1 (en) 2021-10-06 2023-04-20 Johnson & Johnson Consumer Inc. Method of Quantifying Product Impact on Human Microbiome
EP4412765A1 (en) 2021-10-06 2024-08-14 Bayer Aktiengesellschaft Integrated system for chemical, biochemical or molecular biological reactions in a microplate
CN118202069A (zh) 2021-11-05 2024-06-14 豪夫迈·罗氏有限公司 用于检测疟疾的组合物和方法
US20250011882A1 (en) 2021-11-22 2025-01-09 Roche Molecular Systems, Inc. Compositions and Methods for Detecting vanA and/or vanB Genes Associated with Multidrug Resistance
AU2022402153A1 (en) 2021-12-03 2024-06-13 Medicinal Genomics Corporation Psilocybe assay
KR20240141247A (ko) 2022-01-26 2024-09-26 주식회사 씨젠 샘플 내 타겟 핵산을 검출하는 방법
CA3250467A1 (en) 2022-04-15 2023-10-19 Aspen Neuroscience, Inc. METHODS FOR CLASSIFYING THE STATE OF DIFFERENTIATION OF CELLS AND ASSOCIATED COMPOSITIONS OF DIFFERENTIATED CELLS
US12091715B2 (en) 2022-04-21 2024-09-17 Paragon Genomics, Inc. Methods and compositions for reducing base errors of massive parallel sequencing using triseq sequencing
US11680293B1 (en) 2022-04-21 2023-06-20 Paragon Genomics, Inc. Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules
EP4532695A1 (en) 2022-05-25 2025-04-09 Celgene Corporation Methods of manufacturing t cell therapies
WO2024003114A1 (en) 2022-06-29 2024-01-04 Actome Gmbh Detection of biomolecules in single cells
CN119452104A (zh) 2022-06-30 2025-02-14 豪夫迈·罗氏有限公司 用于检测性病淋巴肉芽肿(lgv)沙眼衣原体血清变型的组合物和方法
EP4547839A1 (en) 2022-06-30 2025-05-07 Kapa Biosystems, Inc. Controlling for tagmentation sequencing library insert size using archaeal histone-like proteins
CN115181787A (zh) * 2022-07-01 2022-10-14 广州医科大学附属第一医院（广州呼吸中心） 一种pcr扩增后的产物快速检测方法
EP4555102A1 (en) 2022-07-12 2025-05-21 Topogene Inc. Scalable, submicron-resolution replication of dna arrays
WO2024042042A1 (en) 2022-08-24 2024-02-29 F. Hoffmann-La Roche Ag Compositions and methods for detecting monkeypox virus
AU2023343062A1 (en) 2022-09-13 2025-04-03 Medicinal Genomics Corporation Psilocybe assay
CN120435301A (zh) 2022-09-20 2025-08-05 科斯特-布里私人有限公司(粪便银行) 用于减少炎症性肠病中内源性硫化物的组合物和方法
EP4621068A1 (en) 2022-11-16 2025-09-24 Seegene, Inc. Detection of target nucleic acid by quenching-pto cleavage and extension (q-ptoce) assay
KR20250113422A (ko) 2022-12-08 2025-07-25 주식회사 씨젠 체외 진단용 시약 제품의 연구개발 관리 장치 및 방법
KR20250110851A (ko) 2022-12-08 2025-07-21 주식회사 씨젠 체외 진단용 시약 제품의 연구개발 모듈을 업데이트하는 방법 및 연구 개발 관리 장치
JP2025542121A (ja) 2022-12-25 2025-12-25 エフ． ホフマン－ラ ロシュ アーゲー カルバペネム耐性アシネトバクター・カルコアセチカス－バウマンニ（ａｃｉｎｅｔｏｂａｃｔｅｒ ｃａｌｃｏａｃｅｔｉｃｕｓ－ｂａｕｍａｎｎｉｉ）（ｃｒａｂ）を検出するための組成物および方法
EP4655411A2 (en) 2023-01-26 2025-12-03 DNA Script Enzymatic synthesis of polynucleotide probes
EP4669777A1 (en) 2023-02-25 2025-12-31 F. Hoffmann-La Roche AG COMPOSITIONS AND METHODS FOR DETECTION OF CANDIDA SPECIES
EP4673167A1 (en) 2023-02-27 2026-01-07 Adaptive Biotechnologies Corp. Therapeutic t cell receptors targeting kras g12d
WO2024184165A1 (en) 2023-03-03 2024-09-12 F. Hoffmann-La Roche Ag Compositions and methods for the detection of group b streptococcus ( streptococcus agalactiae) and clindamycin resistance gene determinants
EP4455303A1 (en) * 2023-04-28 2024-10-30 Mobidiag Oy Nucleic acid amplification process controls
CN121263534A (zh) 2023-05-16 2026-01-02 罗氏分子系统公司 使用多模态信号探针检测靶标分析物
WO2025027080A1 (en) 2023-08-03 2025-02-06 F. Hoffmann-La Roche Ag Use of 5'-tailed long primers to improve amplification performance when targeting short primer-binding sites
WO2025072571A1 (en) 2023-09-27 2025-04-03 Cellanome, Inc. Cell culture within microfluidic structures
WO2025202488A1 (en) 2024-03-28 2025-10-02 Roche Diagnostics Gmbh Enhancer of nucleic acid amplification
WO2025245399A1 (en) 2024-05-23 2025-11-27 Indapta Therapeutics, Inc. Method of treating cancer with natural killer cells
WO2025257150A1 (en) 2024-06-11 2025-12-18 Roche Diagnostics International Ag Computer-implemented method for early-stage prediction of pcr result
WO2026006318A1 (en) 2024-06-24 2026-01-02 Amplifidx, Inc. Point-of-care bioassay systems and methods of making and using the same
WO2026022182A1 (en) 2024-07-23 2026-01-29 Institut National de la Santé et de la Recherche Médicale Detection of low allele frequency mutations using allele-specific amplification and crispr/cas13a-based method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05184397A (ja) * 1991-05-02 1993-07-27 F Hoffmann La Roche Ag 核酸増幅および検出のための均質的方法
JPH05237000A (ja) * 1990-10-31 1993-09-17 Tosoh Corp 核酸の検出及び測定方法
JPH07163397A (ja) * 1993-08-27 1995-06-27 F Hoffmann La Roche Ag 複増幅反応の同時観測および分析方法

Family Cites Families (113)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1006767A (en) 1910-01-19 1911-10-24 Gen Electric Electric hair-drier.
US1456005A (en) 1922-07-24 1923-05-22 Harris Jack Incubator
US2379474A (en) 1943-11-10 1945-07-03 Bramson Maurice Heating cabinet, incubator, and the like
US3219416A (en) 1962-10-30 1965-11-23 Scientific Industries Apparatus for the automatic chemical sequential treatment and analysis of small quantities of material
US3556659A (en) 1966-02-03 1971-01-19 Applied Physics Corp Laser-excited raman spectrometer
US3616264A (en) 1969-06-30 1971-10-26 Beckman Instruments Inc Temperature-controlled discrete sample analyzer
US3718133A (en) 1971-01-12 1973-02-27 Damon Corp Container unit for liquid samples
US4038055A (en) 1975-10-10 1977-07-26 Block Engineering, Inc. Gas chromatograph for continuous operation with infrared spectrometer
US4168017A (en) 1977-04-05 1979-09-18 Anderwald Cecil E Container means preventing accidental use by children
AU528259B2 (en) 1978-09-14 1983-04-21 Union Carbide Corp Method and apparatus for assaying liquid materials
IT1101295B (it) 1978-11-22 1985-09-28 Erba Strumentazione Camera gas-cromatografica
US4257774A (en) * 1979-07-16 1981-03-24 Meloy Laboratories, Inc. Intercalation inhibition assay for compounds that interact with DNA or RNA
US4420679A (en) 1982-02-26 1983-12-13 Delta Associates, Inc. Gas chromatographic oven using symmetrical flow of preheated - premixed ambient air
US4468423A (en) 1982-11-17 1984-08-28 Arlie Hall Insulating cell element and structures composed thereof
US4468426A (en) * 1982-11-24 1984-08-28 E. I. Du Pont De Nemours And Company Adherable reinforced poly(ethylene terephthalate) composite sheet
US4481405A (en) 1983-04-27 1984-11-06 Malick Franklin S Cooking appliance
US4599169A (en) 1984-02-28 1986-07-08 Varian Associates, Inc. Heating and cooling apparatus for chromatography column
US4701415A (en) 1984-03-02 1987-10-20 Mallinckrodt, Inc. Controlled atmosphere enclosure
JPS60212986A (ja) 1984-04-06 1985-10-25 日本特殊陶業株式会社 直流電圧印加用セラミツクヒ−タ−
JPS60241884A (ja) 1984-05-15 1985-11-30 Tokyo Daigaku 自動サイクリング反応装置およびこれを用いる自動分析装置
US5038852A (en) 1986-02-25 1991-08-13 Cetus Corporation Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5333675C1 (en) 1986-02-25 2001-05-01 Perkin Elmer Corp Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
JPS6212986A (ja) 1985-07-10 1987-01-21 Hitachi Ltd 磁気バブルメモリデバイスのパツケ−ジ
US4740472A (en) 1985-08-05 1988-04-26 The United States Of America As Represented By The United States Department Of Energy Method and apparatus for automated processing and aliquoting of whole blood samples for analysis in a centrifugal fast analyzer
US4868104A (en) * 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US4675300A (en) 1985-09-18 1987-06-23 The Board Of Trustees Of The Leland Stanford Junior University Laser-excitation fluorescence detection electrokinetic separation
CA1273552A (en) * 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
ATE88761T1 (de) * 1986-01-10 1993-05-15 Amoco Corp Kompetitiver homogener test.
US4868103A (en) 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
CA1339653C (en) 1986-02-25 1998-02-03 Larry J. Johnson Appartus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US4708782A (en) 1986-09-15 1987-11-24 Sepragen Corporation Chromatography column-electrophoresis system
US4684465A (en) 1986-10-10 1987-08-04 Combustion Engineering, Inc. Supercritical fluid chromatograph with pneumatically controlled pump
US4902624A (en) 1987-11-23 1990-02-20 Eastman Kodak Company Temperature cycling cuvette
DE3808942A1 (de) 1988-03-17 1989-09-28 Bio Med Gmbh Ges Fuer Biotechn Inkubator, insbes. fuer die polymerase-ketten-methode
GB8807297D0 (en) 1988-03-26 1988-04-27 Dean P D G Intelligent heating block
US4908112A (en) 1988-06-16 1990-03-13 E. I. Du Pont De Nemours & Co. Silicon semiconductor wafer for analyzing micronic biological samples
US4865986A (en) 1988-10-06 1989-09-12 Coy Corporation Temperature control apparatus
US5169511A (en) 1988-11-29 1992-12-08 Isco, Inc. Capillary electrophoresis technique
US5536648A (en) 1988-12-09 1996-07-16 Amrad Corporation Limited Amplified DNA assay using a double stranded DNA binding protein
WO1990009595A1 (en) 1989-02-08 1990-08-23 City Of Hope Apparatus and method for the sequential performance of chemical processes
DE69012887D1 (de) 1989-06-20 1994-11-03 Claudio Bonini Prüfröhrchen mit einer linsenförmigen Aussenfläche, insbesondere für automatische klinische Analysen.
US5449621A (en) * 1989-07-31 1995-09-12 Biotope, Inc. Method for measuring specific binding assays
US5346672A (en) 1989-11-17 1994-09-13 Gene Tec Corporation Devices for containing biological specimens for thermal processing
US5075556A (en) * 1989-12-01 1991-12-24 Technicon Instruments Corporation Acridine orange derivatives and their use in the quantitation of reticulocytes in whole blood
US5169521A (en) 1989-12-13 1992-12-08 The United States Of America As Represented By The Department Of Health And Human Services Apparatus for countercurrent chromatography separations
US5173163A (en) 1990-01-11 1992-12-22 Isco, Inc. Capillary electrophoresis technique
US5141621A (en) 1990-01-26 1992-08-25 The Board Of Trustees Of The Leland Stanford Junior University Capillary electrophoresis injection device and method
US5207987A (en) * 1990-05-21 1993-05-04 Pb Diagnostic Systems Inc. Temperature controlled chamber for diagnostic analyzer
EP0459241B1 (en) 1990-05-29 1994-10-26 Waters Investments Limited Process and apparatus for effecting capillary electrophoresis
US5455175A (en) 1990-06-04 1995-10-03 University Of Utah Research Foundation Rapid thermal cycling device
US7081226B1 (en) 1996-06-04 2006-07-25 University Of Utah Research Foundation System and method for fluorescence monitoring
US5187084A (en) 1990-06-22 1993-02-16 The Dow Chemical Company Automatic air temperature cycler and method of use in polymerose chain reaction
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5269900A (en) 1990-09-13 1993-12-14 University Of North Carolina At Chapel Hill Method and device for high speed separation of complex molecules
US5131998A (en) 1990-11-13 1992-07-21 The University Of North Carolina At Chapel Hill Two-dimensional high-performance liquid chromatography/capillary electrophoresis
US5240577A (en) 1990-11-13 1993-08-31 University Of North Carolina At Chapel Hill Two-dimensional high-performance liquid chromatography/capillary electrophoresis
KR100236506B1 (ko) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
US5380489A (en) 1992-02-18 1995-01-10 Eastman Kodak Company Element and method for nucleic acid amplification and detection using adhered probes
JP3175941B2 (ja) 1991-03-28 2001-06-11 パーセプティブ バイオシステムズ インコーポレイテッド クロマトグラフ溶出液の減法に依るオンライン生成物確認方法及び装置
WO1992020778A1 (en) 1991-05-24 1992-11-26 Kindconi Pty Limited Biochemical reaction control
US5340715A (en) 1991-06-07 1994-08-23 Ciba Corning Diagnostics Corp. Multiple surface evanescent wave sensor with a reference
US5316913A (en) 1991-09-06 1994-05-31 Stanford University Neutrophil LECAM-1 as indicator of neutrophil activation
US5114551A (en) 1991-09-30 1992-05-19 Bio-Rad Laboratories, Inc. Multi-point detection method for electrophoresis and chromatography in capillaries
US5116471A (en) 1991-10-04 1992-05-26 Varian Associates, Inc. System and method for improving sample concentration in capillary electrophoresis
US5565322A (en) * 1991-11-07 1996-10-15 Nanogen, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to donor energy transfer system
US5348853A (en) 1991-12-16 1994-09-20 Biotronics Corporation Method for reducing non-specific priming in DNA amplification
DE4234086A1 (de) * 1992-02-05 1993-08-12 Diagen Inst Molekularbio Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen
ES2082256T3 (es) 1992-03-23 1996-03-16 Hoffmann La Roche Metodo de deteccion del adn.
CA2133643A1 (en) 1992-04-06 1993-10-14 Stanley R. Bouma Method and device for detection of nucleic acid or analyte using total internal reflectance
JPH0634546A (ja) 1992-07-17 1994-02-08 Tosoh Corp 蛍光検出器
AU5088793A (en) 1992-08-24 1994-03-15 Baxter Diagnostics Inc. Sealable vessel for containing and processing analytical samples
US5340728A (en) * 1992-12-09 1994-08-23 E. I. Du Pont De Nemours And Company Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction
US5593840A (en) 1993-01-27 1997-01-14 Oncor, Inc. Amplification of nucleic acid sequences
US5364790A (en) 1993-02-16 1994-11-15 The Perkin-Elmer Corporation In situ PCR amplification system
US5798215A (en) 1993-02-18 1998-08-25 Biocircuits Corporation Device for use in analyte detection assays
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US6027884A (en) * 1993-06-17 2000-02-22 The Research Foundation Of The State University Of New York Thermodynamics, design, and use of nucleic acid sequences
JP3598123B2 (ja) 1993-07-15 2004-12-08 浜松ホトニクス株式会社 核酸の変性検出装置
EP0636413B1 (en) 1993-07-28 2001-11-14 PE Corporation (NY) Nucleic acid amplification reaction apparatus and method
ATE207966T1 (de) 1993-09-13 2001-11-15 Canon Kk Bestimmung von nukleinsäuren durch pcr, messung der anzahl von mikrobiellen zellen, genen oder genkopien durch pcr und kit zu ihrer verwendung
US5415839A (en) 1993-10-21 1995-05-16 Abbott Laboratories Apparatus and method for amplifying and detecting target nucleic acids
EP2423322A1 (en) * 1993-11-12 2012-02-29 PHRI Properties, Inc. Hybridization probes for nucleic acid detection, universal stems, methods and kits
US5804380A (en) 1993-11-12 1998-09-08 Geron Corporation Telomerase activity assays
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5863726A (en) 1993-11-12 1999-01-26 Geron Corporation Telomerase activity assays
GB9401535D0 (en) 1994-01-27 1994-03-23 Steer Rupert P Timing devices
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
JP3383310B2 (ja) 1994-02-01 2003-03-04 ロバート イー． フィールズ， 分子分析器および使用方法
JP3265113B2 (ja) * 1994-03-04 2002-03-11 三菱製紙株式会社 インクジェット記録シート
CA2143365A1 (en) 1994-03-14 1995-09-15 Hugh V. Cottingham Nucleic acid amplification method and apparatus
JP2909216B2 (ja) 1994-04-29 1999-06-23 パーキン‐エルマー コーポレイション 核酸増幅生成物のリアルタイム検出装置
EP0684316B1 (en) * 1994-04-29 2004-10-20 Johnson & Johnson Clinical Diagnostics, Inc. Homogeneous method for assay of double-stranded nucleic acids using fluorescent dyes and kit useful therein
JPH10500572A (ja) 1994-05-23 1998-01-20 バイオトロニクス コーポレーション 標的核酸を検出する方法
US5766889A (en) 1994-06-08 1998-06-16 The Perkin-Elmer Corporation Method for determining the characteristics of the concentration growth of target nucleic acid molecules in polymerase chain reaction sample
US5622821A (en) 1994-06-29 1997-04-22 The Regents Of The University Of California Luminescent lanthanide chelates and methods of use
EP0711840B1 (en) * 1994-11-09 2002-06-19 Hitachi, Ltd. DNA analyzing method and device therefor
WO1996040992A2 (en) * 1995-06-07 1996-12-19 Abbott Laboratories Probe masking method of reducing background in an amplification reaction
US6008373A (en) * 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
WO1996041864A1 (en) 1995-06-13 1996-12-27 The Regents Of The University Of California Diode laser heated micro-reaction chamber with sample detection means
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5945526A (en) * 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5863727A (en) * 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
EP1033411B1 (en) * 1996-06-04 2006-02-22 University of Utah Research Foundation Fluorescent donor-acceptor pair
US5846727A (en) 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
EP0822261B1 (en) * 1996-07-29 2002-07-03 Academia Sinica Methods for rapid antimicrobial susceptibility testing
US6482590B1 (en) 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
US6143496A (en) * 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6153411A (en) * 1998-10-30 2000-11-28 American Water Works Company, Inc. Methods and kits for detection of Cryptosporidium parvum using immunomagnetic separation and amplification

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05237000A (ja) * 1990-10-31 1993-09-17 Tosoh Corp 核酸の検出及び測定方法
JPH05184397A (ja) * 1991-05-02 1993-07-27 F Hoffmann La Roche Ag 核酸増幅および検出のための均質的方法
JPH07163397A (ja) * 1993-08-27 1995-06-27 F Hoffmann La Roche Ag 複増幅反応の同時観測および分析方法

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