JP2011501965A - 核酸のハイ・スループット塩基配列決定のための装置 - Google Patents
核酸のハイ・スループット塩基配列決定のための装置 Download PDFInfo
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Abstract
Description
本出願は、参照により本明細書に組み込まれる、2007年10月30日に出願された「Apparatus For High Throughput Sequencing Of Nucleic Acids」という表題の米国仮出願第60/983,886号の利益を米国特許法第119条(e)項に従って主張するものである。
・・・ 該当なし。
<<コンパクト・ディスクで提出された「シーケンス・リスティング」、表、またはコンピュータ・プログラム・リスティング付録への参照>>
・・・ 該当なし。
本発明は、一般的に、核酸の自動化された光学的検出の分野に関する。本発明は、一般的に、核酸の自動化されたハイスループット塩基配列決定のための拡張性の高い反応および検出システムを対象とする。
現在の技術に関する十分な背景知識を前提とするうえで、以下の技術用語を理解しておくことが役立つ。
4 作業領域(テーブル); 5 レール; 6 変位ユニット;
7 観察ツール7; 8、8’ 把持メカニズム; 9 搬送デバイス。
Claims (10)
- ハイ・スループット核酸塩基配列決定のためのシステムであって、
反応ユニット内の核酸試料に対し生化学的塩基配列決定反応を実行するための反応サブシステムと、
前記塩基配列決定反応の光学画像を取得し、前記塩基配列決定反応と無関係に前記光学画像の解析に使用する、個別検出サブシステムと、
反応プラットフォームと前記検出システムとの間に前記核酸試料を移動し、それにより前記プラットフォームおよび前記検出システムが可逆的に統合されたシステム内で物理的疎結合される、結合サブシステムとを備えるシステム。 - 前記反応サブシステムは、
複数の個別反応プラットフォームを入れ換えて利用可能であるように構成された少なくとも1つの個別反応プラットフォームを備える請求項1に記載のシステム。 - 前記反応プラットフォームは、
複数の反応ユニットを備え、それぞれの反応ユニットは前記反応プラットフォームから取り外し可能である少なくとも1つの個別フロー・セルを収納する請求項2に記載のシステム。 - 前記結合サブシステムは、
他の個別反応プラットフォームと関係なく前記少なくとも1つの個別反応プラットフォームのうちの選択された1つの反応プラットフォームから前記反応ユニットを選択し、前記反応ユニットを前記検出サブシステムに移動するように動作可能な把持メカニズムを有する搬送デバイスを備える請求項3に記載のシステム。 - 前記把持メカニズムは、反応プラットフォームの作業場に対して垂直な軸を中心として回転可能な移動可能アームを備える請求項4に記載のシステム。
- 検出および処理試薬を保管し、前記検出および処理試薬を前記フロー・セルに移動するための流体工学サブシステムをさらに備える請求項4に記載のシステム。
- 複数の前記反応プラットフォームは、前記検出サブシステムに関して互いに隣接するように並べて配設されている請求項4に記載のシステム。
- 複数の前記反応プラットフォームは、前記検出サブシステムに関して互いに隣接するように前後に並べて配設されている請求項4に記載のシステム。
- 前記少なくとも1つの反応プラットフォームは、光学観察のための蛍光塩基配列決定反応産物として核酸試料を調製するように動作可能であり、
前記検出サブシステムは、コンピュータ援用識別のため前記複数の蛍光反応産物のカラー画像を取り込むように動作可能である請求項4に記載のシステム。 - 核酸上の自動化塩基配列決定反応を処理し検出するための方法であって、
複数のフロー・セルを反応プラットフォーム上に留置し、それぞれのフロー・セルは複数の識別可能な反応部位および前記複数の反応部位に付着された複数の核酸試料を備えることと、
処理試薬を前記フロー・セル内に注入することと、
一定の処理期間の間、前記処理試薬を前記核酸試料と反応させて、蛍光塩基配列決定産物を生成する塩基配列決定反応を引き起こすことと、この後、
前記フロー・セルを前記反応プラットフォームから検出サブシステムに、前記検出サブシステムに物理的に疎結合されている自動化結合サブシステムを使用して移動することと、
前記検出サブシステムにおいて前記フロー・セルを照射することで、前記蛍光塩基配列決定産物に、前記識別可能な反応部位において識別可能なスペクトルで蛍光発光させることと、
前記フロー・セル内の前記識別可能な反応部位から蛍光のカラー画像を取り込み、核酸塩基配列を識別するために使用することとを含み、前記取り込み段階の持続時間は前記反応段階の時間よりも実質的に短い方法。
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US98388607P | 2007-10-30 | 2007-10-30 | |
PCT/US2008/081818 WO2009059022A1 (en) | 2007-10-30 | 2008-10-30 | Apparatus for high throughput sequencing of nucleic acids |
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US (2) | US9382585B2 (ja) |
EP (1) | EP2215209B1 (ja) |
JP (1) | JP2011501965A (ja) |
CN (2) | CN105349647B (ja) |
DK (1) | DK2215209T3 (ja) |
HK (1) | HK1216764A1 (ja) |
SG (1) | SG185935A1 (ja) |
WO (1) | WO2009059022A1 (ja) |
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