ATE348192T1 - Multiplex-hpv-pcr-fluoreszenzassays unter verwendung mehrerer fluorophore - Google Patents

Multiplex-hpv-pcr-fluoreszenzassays unter verwendung mehrerer fluorophore

Info

Publication number
ATE348192T1
ATE348192T1 AT02759447T AT02759447T ATE348192T1 AT E348192 T1 ATE348192 T1 AT E348192T1 AT 02759447 T AT02759447 T AT 02759447T AT 02759447 T AT02759447 T AT 02759447T AT E348192 T1 ATE348192 T1 AT E348192T1
Authority
AT
Austria
Prior art keywords
multiple fluorophores
fluorescence assay
pcr fluorescence
hpv
present
Prior art date
Application number
AT02759447T
Other languages
English (en)
Inventor
Kathrin U Jansen
Frank J Taddeo
Weili Li
Anthony C Dicello
Original Assignee
Merck & Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck & Co Inc filed Critical Merck & Co Inc
Application granted granted Critical
Publication of ATE348192T1 publication Critical patent/ATE348192T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • C12Q1/708Specific hybridization probes for papilloma

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
AT02759447T 2001-08-23 2002-08-19 Multiplex-hpv-pcr-fluoreszenzassays unter verwendung mehrerer fluorophore ATE348192T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US31438301P 2001-08-23 2001-08-23

Publications (1)

Publication Number Publication Date
ATE348192T1 true ATE348192T1 (de) 2007-01-15

Family

ID=23219734

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02759447T ATE348192T1 (de) 2001-08-23 2002-08-19 Multiplex-hpv-pcr-fluoreszenzassays unter verwendung mehrerer fluorophore

Country Status (11)

Country Link
US (1) US20050175987A1 (de)
EP (1) EP1421200B1 (de)
JP (1) JP2005500856A (de)
AT (1) ATE348192T1 (de)
CA (1) CA2457888A1 (de)
CY (1) CY1106353T1 (de)
DE (1) DE60216777T2 (de)
DK (1) DK1421200T3 (de)
ES (1) ES2276948T3 (de)
PT (1) PT1421200E (de)
WO (1) WO2003019143A2 (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7087414B2 (en) 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
ES2282599T3 (es) * 2002-01-07 2007-10-16 Norchip A/S Metodo para detectar arnm del virus del papiloma humano.
US6818420B2 (en) 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
US8323897B2 (en) 2002-12-04 2012-12-04 Applied Biosystems, Llc Multiplex amplification of polynucleotides
CN1294276C (zh) * 2003-07-22 2007-01-10 厦门大学 检测核酸的等长双链特异性探针
EP1687437A4 (de) * 2003-10-13 2008-01-02 Genaco Biomedial Products Inc Verfahren und kit zur auf primern beruhenden amplifikation von nukleinsäuren
WO2006060872A1 (en) 2004-12-10 2006-06-15 Genera Biosystems Pty Ltd Human papilloma virus (hpv) detection using nucleic acid probes, microbeads and fluorescent-activated cell sorter (facs)
KR100705746B1 (ko) 2005-03-14 2007-04-09 학교법인 성균관대학 사람파필로마바이러스의 검출을 위한 프라이머
US8927208B2 (en) * 2005-03-30 2015-01-06 Labo Bio-Medical Investments B.V. Identification of beta-papillomavirus DNA by type-specific reverse hybridization
CA2604692A1 (en) * 2005-04-28 2006-11-02 Merck And Co., Inc. Fluorescent multiplex hpv pcr assays
EP2343385B1 (de) 2006-04-11 2014-05-21 Bio-Rad Innovations HPV-Detektion und Quantifizierung durch Echtzeit-Multiplexverstärkung
US20080050737A1 (en) * 2006-05-23 2008-02-28 Boaz Arieli Ambient Temperature Stable Kits for Molecular Diagnostics
DE102006041970A1 (de) * 2006-08-28 2008-03-06 Genome Identification Diagnostics Gmbh Verfahren und Mittel zum Nachweis von humanen Papillomaviren
EP2142926B1 (de) 2007-04-05 2012-12-05 Genera Biosystems Limited Zusammensetzungen und nachweisverfahren
EP1997914A1 (de) * 2007-06-01 2008-12-03 Università Degli Studi Di Milano - Bicocca Identifizierung und Quantifizierung onkogener HPV-Nukleinsäuren mittels PCR-Assays in Echtzeit
US9435001B2 (en) 2007-11-01 2016-09-06 Self-Screen B.V. Detection method for cervical HPVS
WO2011088573A1 (en) * 2010-01-19 2011-07-28 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Health Set of probes for the detection and typing of 46 human papillomavirus mucosal types
AU2011218215B2 (en) 2010-02-16 2015-10-15 Becton, Dickinson And Company Assay for detecting closely- related serotypes of human papillomavirus (HPV)
EP2661508B1 (de) * 2011-01-07 2018-09-12 QIAGEN Gaithersburg, Inc. Verfahren zur genotypierung und quantifizierung eines hochriskanten menschlichen papillomavirus
KR102478639B1 (ko) 2016-02-23 2022-12-19 노을 주식회사 표지 물질을 저장하는 패치, 이를 이용하는 조직 진단 방법 및 장치
US10371610B2 (en) 2016-02-23 2019-08-06 Noul Co., Ltd. Contact-type patch, staining method using the same, and manufacturing method thereof
KR20170099738A (ko) 2016-02-23 2017-09-01 노을 주식회사 접촉식 염색 패치 및 그 제조 방법
BR112021003434A2 (pt) 2018-09-24 2021-05-18 Flooring Industries Limited, Sarl tinta cerâmica para impressão digital de jato de tinta
WO2021028950A1 (en) * 2019-08-15 2021-02-18 Shrestha Devjani Ghosh Methods for diagnosis and in vitro risk stratification for head and neck cancer based on exosomal mrnas
WO2022115470A1 (en) * 2020-11-25 2022-06-02 Precigen, Inc. Human papilloma virus vaccines and uses of the same for hpv associated diseases
US20250172562A1 (en) * 2022-02-16 2025-05-29 The General Hospital Corporation Contact-type patches for staining
CA3247555A1 (en) * 2022-04-08 2023-10-12 Seegene, Inc. METHOD FOR DETECTING THREE TARGET NUCLEIC ACIDS IN A SAMPLE
CN119570985A (zh) * 2025-02-08 2025-03-07 中国人民解放军军事科学院军事医学研究院 一种基于一步法双通道rpa-crispr的hpv16和hpv18核酸检测试剂盒

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5876922A (en) * 1985-07-31 1999-03-02 Institute Pasteur Papillomavirus probe and a process for in vitro diagnosis of papillomavirus infections
US5447839A (en) * 1988-09-09 1995-09-05 Hoffmann-La Roche Inc. Detection of human papillomavirus by the polymerase chain reaction
US5863717A (en) * 1989-11-03 1999-01-26 Abbott Laboratories Use of conserved oligonucleotide primers to amplify human papillomavirus DNA sequences
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5571673A (en) * 1994-11-23 1996-11-05 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
EP1033411B1 (de) * 1996-06-04 2006-02-22 University of Utah Research Foundation Fluoreszenz-Donor-Akzeptor Paar
US6218105B1 (en) * 1996-07-19 2001-04-17 Kathleen S. Hall High throughput papilloma virus in vitro infectivity assay
US6261771B1 (en) * 1998-02-18 2001-07-17 The United States Of America As Represented By The Secretary Of The Army Method and apparatus for detection of multiple nucleic acid sequences and multiple antigens
US6268143B1 (en) * 1998-08-05 2001-07-31 Kansas State University Research Foundation Automated high throughput E. coli o157:H7 PCR detection system and uses thereof

Also Published As

Publication number Publication date
EP1421200A2 (de) 2004-05-26
WO2003019143A2 (en) 2003-03-06
CA2457888A1 (en) 2003-03-06
ES2276948T3 (es) 2007-07-01
DK1421200T3 (da) 2007-04-10
EP1421200B1 (de) 2006-12-13
DE60216777T2 (de) 2007-10-31
PT1421200E (pt) 2007-02-28
US20050175987A1 (en) 2005-08-11
DE60216777D1 (de) 2007-01-25
WO2003019143A3 (en) 2003-05-08
CY1106353T1 (el) 2011-10-12
EP1421200A4 (de) 2004-12-01
JP2005500856A (ja) 2005-01-13

Similar Documents

Publication Publication Date Title
ATE348192T1 (de) Multiplex-hpv-pcr-fluoreszenzassays unter verwendung mehrerer fluorophore
HRP20100414T1 (hr) Postupak detekcije mutacija egfr u uzorcima krvi
ATE541057T1 (de) Primer und sonden für den nachweis und die unterscheidung von typen und subtypen des influenzavirus
DE60036307D1 (de) Fluoreszenzintensitätstest für duplex- und triplex-nukleinsäurehybridisierung in lösung unter verwendung von fluoreszenzinterkalatoren
ATE448327T1 (de) Verfahren und zusammensetzungen zum nachweis und zur analyse von polynukleotidbindungsproteinwechselwirkungen unter verwendung licht sammelnder multichromophoren
ATE476521T1 (de) Verfahren und zusammensetzungen zum nachweis und zur analyse von polynukleotiden unter verwendung von licht einfangenden multichromophoren
GB2458855A (en) Detection device and methods of use
WO2006014351A3 (en) Exploring fluorophore microenvironments
ES2659798T3 (es) Detección de la restricción de cadena ligera de inmunoglobulina por hibridación in situ de ARN
EP1806410A3 (de) Floureszente, multiplexe HPV-PCR-Assays unter Verwendung multipler Fluorophore
RU2011117297A (ru) Праймеры и зонды для обнаружения вируса папилломы человека и последовательностей бета-глобина человека в исследуемых образцах
ATE307903T1 (de) Immunologischer nachweis von rna:dna hybriden auf mikroarrays
EP3356045A1 (de) Assayplatte und verwendungen davon
Lee et al. A simple, low-cost, and rapid device for a DNA methylation-specific amplification/detection system using a flexible plastic and silicon complex
Alanio et al. Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR
EP1321520A4 (de) Neuartige nukleinsäuresonde und neuartiges verfahren zur analyse von nukleinsäuren unter verwendung derselben
MY149655A (en) Detection and enumeration of microorganisms
WO2001068913A3 (en) Nucleic acid detection method and system
ATE386818T1 (de) Zwei farben differential display als verfahren zur detektion regulierter gene
WO2009011297A1 (ja) Jak2遺伝子の変異検出用プローブおよびその用途
WO2005083394A3 (en) Methods for detecting anionic and non-anionic proteins using carbocyanine dyes
ES2665465T3 (es) Ensayo para detectar serotipos estrechamente relacionados del papilomavirus humano (HPV)
ATE449868T1 (de) Methoden, testsätze und kompositionen zur detektion und quantifizierung von zielsequenzen
WO2012070863A2 (ko) 핵산효소-분자비콘을 이용한 핵산의 검출방법
EP4388132A1 (de) Digitales amplifikationsanalyseverfahren

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1421200

Country of ref document: EP

REN Ceased due to non-payment of the annual fee