ATE259424T1 - Enzym-substrate und assay mit intramolekularen fret - Google Patents

Enzym-substrate und assay mit intramolekularen fret

Info

Publication number
ATE259424T1
ATE259424T1 AT00991947T AT00991947T ATE259424T1 AT E259424 T1 ATE259424 T1 AT E259424T1 AT 00991947 T AT00991947 T AT 00991947T AT 00991947 T AT00991947 T AT 00991947T AT E259424 T1 ATE259424 T1 AT E259424T1
Authority
AT
Austria
Prior art keywords
enzyme substrates
fret
fret assay
moiety
fluorescent
Prior art date
Application number
AT00991947T
Other languages
English (en)
Inventor
Kurt Jarnigan
Roger Leger
Ving J Lee
David Morgans
Original Assignee
Iconix Pharm Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Iconix Pharm Inc filed Critical Iconix Pharm Inc
Application granted granted Critical
Publication of ATE259424T1 publication Critical patent/ATE259424T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Plural Heterocyclic Compounds (AREA)
AT00991947T 1999-11-05 2000-11-03 Enzym-substrate und assay mit intramolekularen fret ATE259424T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16408199P 1999-11-05 1999-11-05
PCT/US2000/041908 WO2001036662A2 (en) 1999-11-05 2000-11-03 Hydrolytic enzyme substrates and assay method

Publications (1)

Publication Number Publication Date
ATE259424T1 true ATE259424T1 (de) 2004-02-15

Family

ID=22592895

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00991947T ATE259424T1 (de) 1999-11-05 2000-11-03 Enzym-substrate und assay mit intramolekularen fret

Country Status (7)

Country Link
US (1) US6455268B1 (de)
EP (1) EP1230380B1 (de)
AT (1) ATE259424T1 (de)
AU (1) AU3642701A (de)
CA (1) CA2388904A1 (de)
DE (1) DE60008290D1 (de)
WO (1) WO2001036662A2 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2809820B1 (fr) * 2000-05-30 2002-10-25 Proteus Methode de detection d'une transformation d'un substrat et ses applications
FR2831888A1 (fr) * 2001-11-08 2003-05-09 Proteus Procede pour generer l'empreinte catalytique idiosyncrasique d'un echantillon et les systemes pour sa mise en oeuvre
FR2831889B1 (fr) * 2001-11-08 2004-01-16 Proteus Procede pour generer l'empreinte catalytique idiosyncrasique d'un echantillon, le traitement de ladite empreinte et les systemes pour leur mise en oeuvre
FR2832729B1 (fr) * 2001-11-28 2004-01-16 Proteus Methode de detection d'une activite catalytique d'un echantillon mettant en oeuvre la detection de la transformation d'un substrat
EP2459227B1 (de) 2009-07-31 2021-03-17 Ascendis Pharma A/S Prodrugs mit einem aromatischen Amin, die durch eine Amidbindung mit einer Trägersubstanz verbunden sind
CN104845611B (zh) * 2015-04-28 2016-06-08 中国科学院理化技术研究所 一种新型氟离子比率荧光探针及应用
WO2017124016A2 (en) * 2016-01-15 2017-07-20 Isi Life Sciences, Inc. Compositions and methods for internalizing pro-labeled molecules into targeted cells and transforming them in situ into labeled molecules
WO2018026965A1 (en) 2016-08-02 2018-02-08 Isi Life Sciences, Inc. Compositions and methods for detecting cancer cells in a tissue sample
US10753942B2 (en) 2017-05-15 2020-08-25 Indicator Systems International, Inc. Methods to detect remnant cancer cells
CN111303865B (zh) * 2018-12-11 2023-03-07 广州中医药大学(广州中医药研究院) 荧光探针化合物及其制备方法和应用
CN114292281B (zh) * 2021-12-22 2023-10-24 东南大学 一种用于实现细胞内脂滴动态成像的小分子荧光探针及其制备方法和应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5208148A (en) * 1990-12-07 1993-05-04 Molecular Probes, Inc. Lipophilic fluorescent glycosidase substrates
US5741657A (en) 1995-03-20 1998-04-21 The Regents Of The University Of California Fluorogenic substrates for β-lactamase and methods of use

Also Published As

Publication number Publication date
WO2001036662A2 (en) 2001-05-25
DE60008290D1 (de) 2004-03-18
EP1230380B1 (de) 2004-02-11
CA2388904A1 (en) 2001-05-25
WO2001036662A3 (en) 2002-05-30
US6455268B1 (en) 2002-09-24
AU3642701A (en) 2001-05-30
EP1230380A2 (de) 2002-08-14

Similar Documents

Publication Publication Date Title
Berndl et al. Comparison of a nucleosidic vs non-nucleosidic postsynthetic “click” modification of DNA with base-labile fluorescent probes
Kim et al. A New Strategy for Fluorogenic Esterase Probes Displaying Low Levels of Non‐specific Hydrolysis
ATE414170T1 (de) Wellenlängen verschiebende proben und primer
ATE259424T1 (de) Enzym-substrate und assay mit intramolekularen fret
WO1999066324A3 (en) A bioluminescence resonance energy transfer (bret) system and its use
ATE236996T1 (de) Sätze von, mit fluoreszierenden energieübertragungsverbindungen, markierten primern und deren verwendung in mehrkomponentenanalyse
ATE263253T1 (de) Nachweis von bakterien und bakteriophagen mit immobilisierten enzymsubstraten
WO2007100392A3 (en) Enzyme substrate comprising a functional dye and associated technology and methods
Ma et al. An FITC‐BODIPY FRET couple: application to selective, ratiometric detection and bioimaging of cysteine
ATE218615T1 (de) Tandem-fluoreszenzproteinkonstrukte
DE60005787D1 (de) Wasserlösliche rhodaminfarbstoffe und ihre konjugate
DE69827152D1 (de) Chemilumineszierende zusammensetzungen und entsprechende verfahren
Thorn-Seshold et al. A robust, high-sensitivity stealth probe for peptidases
DE60143888D1 (de) Methode um die sensitivität einer luminesenz assay zu erhöhen
WO2005005956A3 (en) Fluorophore compounds and their use in biological systems
Ekambaram et al. Benzoselenadiazole-based responsive long-lifetime photoluminescent probes for protein kinases
AR018821A1 (es) Procedimiento para la determinacion de antibioticos con nucleo beta-lactama en un liquido biologico
EP1321520A4 (de) Neuartige nukleinsäuresonde und neuartiges verfahren zur analyse von nukleinsäuren unter verwendung derselben
ATE306082T1 (de) Nachweis der bindung von ladungsträgern mittels ph- oder potential-sensitiver sonden
SE9901306D0 (sv) Improved TIRF chamber
ATE288922T1 (de) Bestimmte heteroaryl substituierte thiol inhibitoren von endothelin wechselnden enzymen
ATE340270T1 (de) Fluoreszenzmarkierten hybridisierungssonden mit reduzierter hintergrundfluoreszenz
ATE501274T1 (de) Cucurbiturilderivat-gebundenes feststoffsubstrat und biochip unter verwendung davon
ATE371747T1 (de) Dendritische chemilumineszierende substrate
DE602005013757D1 (de) Enzymsubstrate für fluoreszenzresonanz-energietransfer

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties