DE60143888D1 - Methode um die sensitivität einer luminesenz assay zu erhöhen - Google Patents

Methode um die sensitivität einer luminesenz assay zu erhöhen

Info

Publication number
DE60143888D1
DE60143888D1 DE60143888T DE60143888T DE60143888D1 DE 60143888 D1 DE60143888 D1 DE 60143888D1 DE 60143888 T DE60143888 T DE 60143888T DE 60143888 T DE60143888 T DE 60143888T DE 60143888 D1 DE60143888 D1 DE 60143888D1
Authority
DE
Germany
Prior art keywords
fold
reduces
sensitivity
luminescence
assay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60143888T
Other languages
English (en)
Inventor
Erika Hawkins
John M Centanni
Jacqueline Sankbeil
Keith V Wood
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24364121&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60143888(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of DE60143888D1 publication Critical patent/DE60143888D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90241—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Plural Heterocyclic Compounds (AREA)
DE60143888T 2000-06-09 2001-06-07 Methode um die sensitivität einer luminesenz assay zu erhöhen Expired - Lifetime DE60143888D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/590,884 US7118878B1 (en) 2000-06-09 2000-06-09 Method for increasing luminescence assay sensitivity
PCT/US2001/018363 WO2001096862A2 (en) 2000-06-09 2001-06-07 Method for increasing luminescence assay sensitivity

Publications (1)

Publication Number Publication Date
DE60143888D1 true DE60143888D1 (de) 2011-03-03

Family

ID=24364121

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60143888T Expired - Lifetime DE60143888D1 (de) 2000-06-09 2001-06-07 Methode um die sensitivität einer luminesenz assay zu erhöhen

Country Status (8)

Country Link
US (3) US7118878B1 (de)
EP (1) EP1297337B1 (de)
JP (2) JP4685325B2 (de)
AT (1) ATE496298T1 (de)
AU (2) AU7532501A (de)
CA (1) CA2411179A1 (de)
DE (1) DE60143888D1 (de)
WO (1) WO2001096862A2 (de)

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US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
AU2003267245C1 (en) * 2002-09-20 2008-05-29 Promega Corporation Luminescence-based methods and probes for measuring cytochrome P450 activity
US7741067B2 (en) 2002-12-23 2010-06-22 Promega Corporation Luciferase-based assays
CA2515217A1 (en) * 2003-02-12 2004-08-26 Promega Corporation Methods and kits for dual enzymatic assays whereby light is quenched from luminescent reactions
EP3312292B1 (de) 2005-05-31 2019-09-25 Promega Corporation Luminogene und fluorogene verbindungen und verfahren für den nachweis von molekülen oder leiden
US20100129840A1 (en) * 2006-10-13 2010-05-27 Charm Scineces, Inc Method and Apparatus for Reducing Luminescent Test Result Interferences
US20080248511A1 (en) * 2007-03-26 2008-10-09 Promega Corporation Methods to quench light from optical reactions
WO2009032218A1 (en) * 2007-08-29 2009-03-12 Biolume Inc. Bioluminescent endoscopy methods and compounds
CA2703785C (en) * 2007-10-29 2017-07-04 Perkinelmer Health Sciences B.V. Methods, reagents and kits for luciferase assay
JP5597200B2 (ja) * 2008-08-18 2014-10-01 プロメガ コーポレイション ルミネッセンスを生じる化合物およびチトクロムｐ４５０３ａ酵素を検出するための方法
KR101665352B1 (ko) 2009-05-01 2016-10-12 프로메가 코포레이션 광 출력이 향상된 합성 오플로포러스 루시퍼라제
EP3498709A1 (de) 2010-11-02 2019-06-19 Promega Corporation Aus oplophorus gewonnene luciferasen, neuartige coelenterazinsubstrate und verfahren zur verwendung
CN103180324A (zh) 2010-11-02 2013-06-26 普罗美加公司 腔肠素衍生物及其使用方法
EP2652142B1 (de) * 2010-12-14 2017-03-01 Stichting Katholieke Universiteit Chemilumineszenzbasierter Hämostasetest
WO2012112482A1 (en) * 2011-02-15 2012-08-23 3M Innovative Properties Company Method of controlling a decontamination process
JP6240162B2 (ja) 2012-05-02 2017-11-29 チャールズ リバー ラボラトリーズ， インコーポレイテッド 細胞収集システムおよびその使用
JP6285915B2 (ja) 2012-05-02 2018-02-28 チャールズ リバー ラボラトリーズ， インコーポレイテッド 生死判別染色法
JP6482459B2 (ja) 2012-05-02 2019-03-13 チャールズ リバー ラボラトリーズ， インコーポレイテッド 細胞サンプル中の生存細胞を検出する方法
WO2015116867A1 (en) 2014-01-29 2015-08-06 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
JP6588917B2 (ja) 2014-01-29 2019-10-09 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 生細胞への適用のためのプロ基質
EP3428277B1 (de) 2016-03-11 2023-05-10 National Institute of Advanced Industrial Science and Technology Lichtemittierendes enzymprotein
EP3661932B1 (de) 2017-08-04 2021-10-06 Promega Corporation Zusammensetzungen und verfahren zur stabilisierung von benzothiazol-luciferin-analoga
WO2020210658A1 (en) * 2019-04-10 2020-10-15 Promega Corporation Compositions and methods for analyte detection using bioluminescence

Family Cites Families (28)

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Publication number Priority date Publication date Assignee Title
SE428379B (sv) 1978-05-31 1983-06-27 Lkb Produkter Ab Bioluminiscens bestemning av atp och reagens herfor
DE2828658C3 (de) 1978-06-29 1981-10-22 Lkb-Produkter Ab, Stockholm Verfahren zur photometrischen Bestimmung der Untereinheit B der Creatinkinase und Reagens hierfür
DE2901919A1 (de) 1979-01-18 1980-07-24 Berthold Lab Prof Dr Anwendung eines - insbesondere automatischen - verfahrens zur lichtmessung sowie weiterbildungen dieses verfahrens und vorrichtungen zu dessen ausfuehrung
SE432112B (sv) * 1979-07-12 1984-03-19 Lkb Produkter Ab Forfarande for bestemning av kreatinkinas i prover innehallande atp
DE3048884A1 (de) 1980-12-23 1982-07-15 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur enzymimmunobestimmung in heterogener phase
US4650770A (en) * 1981-04-27 1987-03-17 Syntex (U.S.A.) Inc. Energy absorbing particle quenching in light emitting competitive protein binding assays
AU7399487A (en) 1986-05-22 1987-12-22 Unilever Plc Solid phase immunoassay method
GB8811294D0 (en) 1988-05-12 1988-06-15 Kabivitrum Peptide Hormones Ab Luminescent/luminometric assays
DE69026851T2 (de) * 1989-02-14 1996-10-31 Wako Pure Chem Ind Ltd Verfahren zur Erhöhung von Chemilumineszenz
FI901681A0 (fi) * 1989-04-10 1990-04-03 Ela Technologies Inc Foerfarande foer oekning av kaensligheten hos luminescensanalyser.
JPH03167288A (ja) 1989-11-27 1991-07-19 Chisso Corp 界面活性剤によるエクオリンの増感発光法
US5283179A (en) 1990-09-10 1994-02-01 Promega Corporation Luciferase assay method
US5629168A (en) * 1992-02-10 1997-05-13 British Technology Group Limited Chemiluminescent enhancers
AT401526B (de) 1993-02-10 1996-09-25 Scheirer Winfried Reagenzlösung zur stabilisierung der lumineszenz bei der luciferasemessung
CA2104815A1 (en) * 1993-02-26 1994-08-27 Naotaka Kuroda Method for measuring adenyl group-containing substances
JPH0767696A (ja) 1993-09-06 1995-03-14 Tosoh Corp バックグランド発光の低減法
US5744320A (en) * 1995-06-07 1998-04-28 Promega Corporation Quenching reagents and assays for enzyme-mediated luminescence
US6602657B1 (en) 1995-12-28 2003-08-05 Tropix, Inc. Multiple reporter gene assay
US5795729A (en) * 1996-02-05 1998-08-18 Biometric Imaging, Inc. Reductive, energy-transfer fluorogenic probes
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US5814504A (en) * 1996-08-22 1998-09-29 Kikkoman Corporation Protein involved in regenerating firefly luciferin
JPH1087621A (ja) 1996-09-13 1998-04-07 Sankyo Co Ltd ルシゲニン化学発光の増強剤
US6200762B1 (en) 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
US6221612B1 (en) 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
DE69915468T2 (de) 1999-09-01 2005-01-20 Invitrogen Corp., Carlsbad Photon Dämpfer für Fluoreszenzassays
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
US6867005B2 (en) * 2001-10-24 2005-03-15 Beckman Coulter, Inc. Method and apparatus for increasing the dynamic range and accuracy of binding assays
CA2515217A1 (en) 2003-02-12 2004-08-26 Promega Corporation Methods and kits for dual enzymatic assays whereby light is quenched from luminescent reactions

Also Published As

Publication number Publication date
JP2004503777A (ja) 2004-02-05
EP1297337B1 (de) 2011-01-19
WO2001096862A2 (en) 2001-12-20
WO2001096862A3 (en) 2002-07-18
US20060051827A1 (en) 2006-03-09
AU2001275325B2 (en) 2006-10-05
US7078181B2 (en) 2006-07-18
CA2411179A1 (en) 2001-12-20
US7118878B1 (en) 2006-10-10
JP4685325B2 (ja) 2011-05-18
US7108996B2 (en) 2006-09-19
ATE496298T1 (de) 2011-02-15
JP2011064690A (ja) 2011-03-31
EP1297337A2 (de) 2003-04-02
US20040096924A1 (en) 2004-05-20
AU7532501A (en) 2001-12-24

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Legal Events

Date Code Title Description
8328 Change in the person/name/address of the agent

Representative=s name: VONNEMANN, KLOIBER & KOLLEGEN, 20099 HAMBURG