ATE340270T1 - Fluoreszenzmarkierten hybridisierungssonden mit reduzierter hintergrundfluoreszenz - Google Patents

Fluoreszenzmarkierten hybridisierungssonden mit reduzierter hintergrundfluoreszenz

Info

Publication number
ATE340270T1
ATE340270T1 AT03016378T AT03016378T ATE340270T1 AT E340270 T1 ATE340270 T1 AT E340270T1 AT 03016378 T AT03016378 T AT 03016378T AT 03016378 T AT03016378 T AT 03016378T AT E340270 T1 ATE340270 T1 AT E340270T1
Authority
AT
Austria
Prior art keywords
fluorescence
entity
hybridization probe
labeled hybridization
reduced background
Prior art date
Application number
AT03016378T
Other languages
English (en)
Inventor
Dieter Dr Heindl
Hans-Peter Dr Josel
Gregor Dr Sagner
Jutta Dr Mayr
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE340270T1 publication Critical patent/ATE340270T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT03016378T 2002-07-23 2003-07-19 Fluoreszenzmarkierten hybridisierungssonden mit reduzierter hintergrundfluoreszenz ATE340270T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP02016265 2002-07-23

Publications (1)

Publication Number Publication Date
ATE340270T1 true ATE340270T1 (de) 2006-10-15

Family

ID=30775784

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03016378T ATE340270T1 (de) 2002-07-23 2003-07-19 Fluoreszenzmarkierten hybridisierungssonden mit reduzierter hintergrundfluoreszenz

Country Status (7)

Country Link
US (1) US20050176014A1 (de)
EP (1) EP1384789B1 (de)
JP (1) JP3970816B2 (de)
AT (1) ATE340270T1 (de)
CA (1) CA2433473A1 (de)
DE (1) DE60308454T2 (de)
ES (1) ES2271436T3 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006034036A1 (en) 2004-09-16 2006-03-30 Applera Corporation Fluorescent dye compounds, conjugates and uses thereof
WO2010117420A2 (en) * 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
KR200452369Y1 (ko) * 2009-05-12 2011-02-22 주식회사 아폴로산업 수도꼭지용 호스-커플링장치
DE102010003782B4 (de) 2010-04-08 2023-09-28 Ist Innuscreen Gmbh Vorrichtung zum Nachweis von Nukleinsäuren
DE102010003781B4 (de) 2010-04-08 2012-08-16 Aj Innuscreen Gmbh Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen
WO2012092403A1 (en) 2010-12-29 2012-07-05 Life Technologies Corporation Ddao compounds as fluorescent reference standards
US9416153B2 (en) 2011-10-11 2016-08-16 Enzo Life Sciences, Inc. Fluorescent dyes
GB2526445A (en) * 2014-07-14 2015-11-25 Identigen Ltd A method for detecting PCR amplification in a sample
EP2975136A1 (de) * 2014-07-14 2016-01-20 Parlanca Limited Verfahren zum Nachweis von PCR-Amplifizierung in einer Probe
WO2018162986A2 (en) 2017-03-09 2018-09-13 Elitechgroup B.V. Nitrodiarylethenes as fluorescence quenchers for nucleic acid probes
ES2909351T3 (es) 2017-08-17 2022-05-06 Elitechgroup Inc Quencheres estabilizadores de fluorescencia dúplex para sondas de ácidos nucleicos

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2039517C (en) * 1990-04-03 2006-11-07 David Segev Dna probe signal amplification
US5945526A (en) * 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
WO1997046707A2 (en) * 1996-06-04 1997-12-11 University Of Utah Research Foundation System and method for monitoring for dna amplification by fluorescence
US6037130A (en) * 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits
US6355435B1 (en) * 1999-09-10 2002-03-12 Board Of Trustees Of Michigan State University Methods for detecting and enumerating Campylobacter jejuni in environmental samples and for identifying antibiotic-resistant strains
EP1307592B1 (de) * 2000-08-11 2006-07-05 University of Utah Research Foundation Einfach markierte oligonukleotidsonden
US7081336B2 (en) * 2001-06-25 2006-07-25 Georgia Tech Research Corporation Dual resonance energy transfer nucleic acid probes

Also Published As

Publication number Publication date
DE60308454D1 (de) 2006-11-02
EP1384789B1 (de) 2006-09-20
CA2433473A1 (en) 2004-01-23
EP1384789A1 (de) 2004-01-28
ES2271436T3 (es) 2007-04-16
DE60308454T2 (de) 2007-05-03
JP2004049241A (ja) 2004-02-19
US20050176014A1 (en) 2005-08-11
JP3970816B2 (ja) 2007-09-05

Similar Documents

Publication Publication Date Title
ATE450623T1 (de) Modifikatoren der lichtemission und ihre verwendungen zur detektion, amplifikation und analyse von nukleinsäuren
DE69939902D1 (de) Wellenlängen verschiebende proben und primer
ATE236996T1 (de) Sätze von, mit fluoreszierenden energieübertragungsverbindungen, markierten primern und deren verwendung in mehrkomponentenanalyse
ATE394508T1 (de) Late-pcr
ATE340270T1 (de) Fluoreszenzmarkierten hybridisierungssonden mit reduzierter hintergrundfluoreszenz
ATE332398T1 (de) Einfach markierte oligonukleotidsonden
DE60143888D1 (de) Methode um die sensitivität einer luminesenz assay zu erhöhen
EP1295941A4 (de) NEUE NUKLEINSäURESONDEN UND VERFAHREN ZUM TESTEN VON NUKLEINSäUREN UNTER DEREN VERWENDUNG
ATE318327T1 (de) Fluoreszenz-donor-akzeptor paar
AU8288101A (en) Real-time sequence determination
ATE354673T1 (de) Direkte quantifizierung von gering vorkommender rns
DE60042738D1 (de) Verfahren zum nachweis von analyten mit hilfe von halbleiternanokrystallen
ATE306669T1 (de) Nitroreduktase reporter-gen assay mit fluoreszenz erhöhung
AU2002221848A1 (en) Dye pair for fluorescence resonance energy transfer (fret) measurements
Ranasinghe et al. Ultrasensitive fluorescence-based methods for nucleic acid detection: towards amplification-free genetic analysis
EA200300880A1 (ru) Способ детекции нуклеотидного полиморфизма
CA2397545A1 (en) Method of detecting dna by dna hybridization method with the use of fluorescent resonance energy transfer
Hannestad et al. Self‐assembled DNA‐based fluorescence waveguide with selectable output
DE60334923D1 (de) Tät
Laitala et al. Time-resolved detection probe for homogeneous nucleic acid analyses in one-step format
ATE286144T1 (de) VERWENDUNG VON FLUORESZIERENDEN ßMOLECULAR BEACONSß ZUM NACHWEIS VON METHYLIERTEN NUKLEINSÄUREN
DE60222091D1 (de) Dendritische chemilumineszierende Substrate
DE60008290D1 (de) Enzym-substrate und assay mit intramolekularen fret
WO2009016718A1 (ja) 診断薬及びそれを用いた診断方法
ES2371664T3 (es) Ensayo de ácidos nucleicos de proximidad de quimioluminiscencia.

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1384789

Country of ref document: EP

REN Ceased due to non-payment of the annual fee