ATE306669T1 - Nitroreduktase reporter-gen assay mit fluoreszenz erhöhung - Google Patents

Nitroreduktase reporter-gen assay mit fluoreszenz erhöhung

Info

Publication number
ATE306669T1
ATE306669T1 AT01902521T AT01902521T ATE306669T1 AT E306669 T1 ATE306669 T1 AT E306669T1 AT 01902521 T AT01902521 T AT 01902521T AT 01902521 T AT01902521 T AT 01902521T AT E306669 T1 ATE306669 T1 AT E306669T1
Authority
AT
Austria
Prior art keywords
nitroreductase
gene assay
fluorescence increase
group
reporter
Prior art date
Application number
AT01902521T
Other languages
English (en)
Inventor
Nicholas Thomas
Nigel Paul Michael
Valerie Millar
Beth Davies
Mark Briggs
Original Assignee
Amersham Biosciences Uk Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Uk Ltd filed Critical Amersham Biosciences Uk Ltd
Application granted granted Critical
Publication of ATE306669T1 publication Critical patent/ATE306669T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
    • C09B67/0033—Blends of pigments; Mixtured crystals; Solid solutions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Inorganic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT01902521T 2000-02-02 2001-02-01 Nitroreduktase reporter-gen assay mit fluoreszenz erhöhung ATE306669T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0002261.6A GB0002261D0 (en) 2000-02-02 2000-02-02 Fluorescent detection method & reagent
PCT/GB2001/000396 WO2001057237A2 (en) 2000-02-02 2001-02-01 Methods employing increased dye-fluorescence upon nitroreduction

Publications (1)

Publication Number Publication Date
ATE306669T1 true ATE306669T1 (de) 2005-10-15

Family

ID=9884728

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01902521T ATE306669T1 (de) 2000-02-02 2001-02-01 Nitroreduktase reporter-gen assay mit fluoreszenz erhöhung

Country Status (12)

Country Link
US (1) US7662973B2 (de)
EP (2) EP1252520B1 (de)
JP (2) JP3984475B2 (de)
AT (1) ATE306669T1 (de)
AU (2) AU782381B2 (de)
CA (1) CA2399368C (de)
DE (1) DE60115205T2 (de)
DK (1) DK1252520T3 (de)
ES (1) ES2250349T3 (de)
GB (1) GB0002261D0 (de)
IL (1) IL150947A0 (de)
WO (1) WO2001057237A2 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050119490A1 (en) * 2001-09-27 2005-06-02 Masayuki Kawakami Asymmetric cyanine dye
GB0123856D0 (en) * 2001-10-05 2001-11-28 Amersham Pharm Biotech Uk Ltd Method for determining cell cycle position
WO2003082988A1 (en) * 2002-03-29 2003-10-09 The General Hospital Corporation Nir-fluorescent cyanine dyes, their synthesis and biological use
WO2004043417A2 (en) * 2002-11-14 2004-05-27 L'oreal Composition for dyeing keratin fibres containing a dye precursor and an enzyme with nitroreductase activity
FR2847161B1 (fr) * 2002-11-14 2005-01-07 Oreal Composition de teinture des fibres keratiniques contenant un precurseur de colorant et une enzyme a activite nitroreductase et procede mettant en oeuvre cette composition
EP1716246A2 (de) * 2004-02-09 2006-11-02 Novozymes A/S Verfahren zum testen oder screening auf ein interessierendes enzym
ES2324721T3 (es) * 2004-04-23 2009-08-13 Ge Healthcare As Sustratos enzimaticos de transferencia de energia de resonancia de fluorescencia.
GB0411993D0 (en) * 2004-05-28 2004-06-30 Amersham Biosciences Uk Ltd Method and reagent for measuring nitroreductase enzyme activity
US7767834B2 (en) * 2004-08-13 2010-08-03 Elitech Holding B.V. Phosphonylated fluorescent dyes and conjugates
WO2006020947A2 (en) 2004-08-13 2006-02-23 Epoch Biosciences, Inc. Phosphonate fluorescent dyes and conjugates
WO2006035207A2 (en) * 2004-09-30 2006-04-06 Ge Healthcare Uk Limited Fluorescent nucleotide analogues
GB0505777D0 (en) * 2005-03-22 2005-04-27 Amersham Biosciences Uk Ltd Enzyme detection method and reagent
WO2007059779A2 (en) * 2005-11-28 2007-05-31 Dako Denmark A/S Cyanine dyes and methods for detecting a target using said dyes
US20100173332A1 (en) * 2006-09-07 2010-07-08 Auckland Uniservices Limited Method for the Fluorescent Detection of Nitroreductase Activity Using Nitro-Substituted Aromatic Compounds
US20080226160A1 (en) * 2007-03-12 2008-09-18 Conversion Works, Inc. Systems and methods for filling light in frames during 2-d to 3-d image conversion
US7906106B2 (en) * 2007-06-27 2011-03-15 General Electric Company In vivo cell trafficking
US8021647B2 (en) * 2007-06-29 2011-09-20 General Electric Company In vivo optical imaging
CA2802087A1 (en) 2010-06-15 2011-12-22 Cellular Dynamics International, Inc. A compendium of ready-built stem cell models for interrogation of biological response
US8828678B2 (en) 2010-11-16 2014-09-09 Enzo Life Sciences, Inc. Self-immolative probes for enzyme activity detection
WO2013009825A1 (en) 2011-07-11 2013-01-17 Cellular Dynamics International, Inc. Methods for cell reprogramming and genome engineering
CN102516793B (zh) * 2011-11-22 2014-02-05 大连理工大学 一类双苄基五甲川菁荧光染料、其制备方法及应用
US20160010094A1 (en) 2013-03-01 2016-01-14 University Of Pretoria Transgenic cell selection
US11662312B2 (en) * 2017-06-27 2023-05-30 Coriolis Pharma Research GmbH Polysorbate quantification assay

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1004428A1 (ru) * 1981-12-30 1983-03-15 Предприятие П/Я Р-6496 4-Амино-N-пиразолилнафталимиды в качестве органических люминофоров желто-зеленого свечени
WO1993008288A1 (en) * 1991-10-23 1993-04-29 Cancer Research Campaign Technology Limited Bacterial nitroreductase for the reduction of cb 1954 and analogues thereof to a cytotoxic form
US5658751A (en) * 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5534416A (en) * 1993-04-13 1996-07-09 Molecular Probes, Inc. Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes
DE69821289T2 (de) * 1997-06-24 2004-11-25 Eastman Kodak Co. Sensibilisierungsfarbstoffe für verbesserte Lichtabsorption
WO1999051270A1 (en) * 1998-04-06 1999-10-14 Dalhousie University A novel nitroreductase and therapeutic uses therefor
GB9812596D0 (en) * 1998-06-11 1998-08-12 Amersham Pharm Biotech Uk Ltd Energy transfer assay method

Also Published As

Publication number Publication date
EP1252520B1 (de) 2005-10-12
AU3037601A (en) 2001-08-14
US20030186348A1 (en) 2003-10-02
AU782381B2 (en) 2005-07-21
GB0002261D0 (en) 2000-03-22
ES2250349T3 (es) 2006-04-16
JP2003522333A (ja) 2003-07-22
EP1252520A2 (de) 2002-10-30
IL150947A0 (en) 2003-02-12
DE60115205T2 (de) 2006-07-20
JP2007183284A (ja) 2007-07-19
WO2001057237A3 (en) 2002-05-16
WO2001057237A2 (en) 2001-08-09
EP1698900A1 (de) 2006-09-06
CA2399368C (en) 2007-04-10
DE60115205D1 (de) 2005-12-29
DK1252520T3 (da) 2006-02-06
AU2005222569A1 (en) 2005-11-10
JP3984475B2 (ja) 2007-10-03
CA2399368A1 (en) 2001-08-09
US7662973B2 (en) 2010-02-16

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