ATE287969T1 - Amplifizierungsverfahen zum nachweis von zielnukleinsäuren unter verwendung von fluoreszenzenergieübertragung - Google Patents

Amplifizierungsverfahen zum nachweis von zielnukleinsäuren unter verwendung von fluoreszenzenergieübertragung

Info

Publication number
ATE287969T1
ATE287969T1 AT00951700T AT00951700T ATE287969T1 AT E287969 T1 ATE287969 T1 AT E287969T1 AT 00951700 T AT00951700 T AT 00951700T AT 00951700 T AT00951700 T AT 00951700T AT E287969 T1 ATE287969 T1 AT E287969T1
Authority
AT
Austria
Prior art keywords
label
target nucleic
sample
sequence
nucleic acids
Prior art date
Application number
AT00951700T
Other languages
English (en)
Inventor
Martin Alan Lee
Dario Lyall Leslie
Original Assignee
Secr Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Secr Defence filed Critical Secr Defence
Application granted granted Critical
Publication of ATE287969T1 publication Critical patent/ATE287969T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
AT00951700T 1999-08-04 2000-08-03 Amplifizierungsverfahen zum nachweis von zielnukleinsäuren unter verwendung von fluoreszenzenergieübertragung ATE287969T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9918237.0A GB9918237D0 (en) 1999-08-04 1999-08-04 Detection system
PCT/GB2000/003016 WO2001011078A1 (en) 1999-08-04 2000-08-03 Amplification method for detection of target nucleic acids involving fluorescence energy transfer

Publications (1)

Publication Number Publication Date
ATE287969T1 true ATE287969T1 (de) 2005-02-15

Family

ID=10858467

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00951700T ATE287969T1 (de) 1999-08-04 2000-08-03 Amplifizierungsverfahen zum nachweis von zielnukleinsäuren unter verwendung von fluoreszenzenergieübertragung

Country Status (10)

Country Link
US (1) US7015018B1 (de)
EP (1) EP1198593B1 (de)
JP (1) JP4628625B2 (de)
AT (1) ATE287969T1 (de)
AU (1) AU774766B2 (de)
CA (1) CA2381037C (de)
DE (1) DE60017750T2 (de)
ES (1) ES2245315T3 (de)
GB (1) GB9918237D0 (de)
WO (1) WO2001011078A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1377683A4 (de) * 2001-04-11 2004-09-08 Us Gov Health & Human Serv Modifizierte zufallsprimer zur sondenmarkierung
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
EP1509624A2 (de) * 2002-05-31 2005-03-02 Secretary, Department Of Atomic Energy Methode für den nukleinsäurenachweis basierend auf met/fret wobei sich die fluorophoren gruppen auf komplementären strängen befinden
US8658366B2 (en) 2008-09-18 2014-02-25 Roche Molecular Systems, Inc. Detection of target variants using a fluorescent label and a soluble quencher
JP5856984B2 (ja) 2011-01-26 2016-02-10 オリンパス株式会社 核酸分子の多型識別方法
WO2012102326A1 (ja) 2011-01-26 2012-08-02 オリンパス株式会社 核酸分子の多型識別方法
CN112266950A (zh) * 2020-10-23 2021-01-26 深圳澳东检验检测科技有限公司 一种探针引物组合及其检测试剂盒

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2082256T3 (es) * 1992-03-23 1996-03-16 Hoffmann La Roche Metodo de deteccion del adn.
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
GB9725237D0 (en) * 1997-11-29 1998-01-28 Secr Defence Amplification system
GB9803382D0 (en) * 1998-02-19 1998-04-15 Secr Defence Detection system
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6440675B1 (en) * 1999-09-17 2002-08-27 Whitehead Institute For Biomedical Research Methods for selecting primers

Also Published As

Publication number Publication date
CA2381037A1 (en) 2001-02-15
JP4628625B2 (ja) 2011-02-09
AU774766B2 (en) 2004-07-08
GB9918237D0 (en) 1999-10-06
DE60017750T2 (de) 2005-12-29
WO2001011078A8 (en) 2001-06-14
AU6455800A (en) 2001-03-05
US7015018B1 (en) 2006-03-21
ES2245315T3 (es) 2006-01-01
CA2381037C (en) 2011-04-12
WO2001011078A1 (en) 2001-02-15
DE60017750D1 (de) 2005-03-03
EP1198593B1 (de) 2005-01-26
EP1198593A1 (de) 2002-04-24
JP2003506068A (ja) 2003-02-18

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