ATE287969T1 - Amplifizierungsverfahen zum nachweis von zielnukleinsäuren unter verwendung von fluoreszenzenergieübertragung - Google Patents
Amplifizierungsverfahen zum nachweis von zielnukleinsäuren unter verwendung von fluoreszenzenergieübertragungInfo
- Publication number
- ATE287969T1 ATE287969T1 AT00951700T AT00951700T ATE287969T1 AT E287969 T1 ATE287969 T1 AT E287969T1 AT 00951700 T AT00951700 T AT 00951700T AT 00951700 T AT00951700 T AT 00951700T AT E287969 T1 ATE287969 T1 AT E287969T1
- Authority
- AT
- Austria
- Prior art keywords
- label
- target nucleic
- sample
- sequence
- nucleic acids
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 230000003321 amplification Effects 0.000 title abstract 4
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 4
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 239000000523 sample Substances 0.000 abstract 6
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 125000005647 linker group Chemical group 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Eye Examination Apparatus (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Geophysics And Detection Of Objects (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9918237.0A GB9918237D0 (en) | 1999-08-04 | 1999-08-04 | Detection system |
| PCT/GB2000/003016 WO2001011078A1 (en) | 1999-08-04 | 2000-08-03 | Amplification method for detection of target nucleic acids involving fluorescence energy transfer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE287969T1 true ATE287969T1 (de) | 2005-02-15 |
Family
ID=10858467
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00951700T ATE287969T1 (de) | 1999-08-04 | 2000-08-03 | Amplifizierungsverfahen zum nachweis von zielnukleinsäuren unter verwendung von fluoreszenzenergieübertragung |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7015018B1 (de) |
| EP (1) | EP1198593B1 (de) |
| JP (1) | JP4628625B2 (de) |
| AT (1) | ATE287969T1 (de) |
| AU (1) | AU774766B2 (de) |
| CA (1) | CA2381037C (de) |
| DE (1) | DE60017750T2 (de) |
| ES (1) | ES2245315T3 (de) |
| GB (1) | GB9918237D0 (de) |
| WO (1) | WO2001011078A1 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002083922A2 (en) * | 2001-04-11 | 2002-10-24 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services | Modified random primers for probe labeling |
| GB0112868D0 (en) * | 2001-05-25 | 2001-07-18 | Secr Defence | Detection system |
| JP4457001B2 (ja) * | 2002-05-31 | 2010-04-28 | セクレタリー・デパートメント・オブ・アトミック・エナジー | ドナー／アクセプター部分が相補鎖上となる標的核酸検出のｍｅｔ／ｆｒｅｔに基づく方法 |
| US8658366B2 (en) * | 2008-09-18 | 2014-02-25 | Roche Molecular Systems, Inc. | Detection of target variants using a fluorescent label and a soluble quencher |
| EP2669376B1 (de) | 2011-01-26 | 2017-08-16 | Olympus Corporation | Verfahren zur identifizierung von polymorphismen von nukleinsäuremolekülen |
| WO2012102260A1 (ja) * | 2011-01-26 | 2012-08-02 | オリンパス株式会社 | 核酸分子の多型識別方法 |
| CN112266950A (zh) * | 2020-10-23 | 2021-01-26 | 深圳澳东检验检测科技有限公司 | 一种探针引物组合及其检测试剂盒 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0566751B1 (de) * | 1992-03-23 | 1996-01-10 | F. Hoffmann-La Roche Ag | Verfahren zum DNS-Nachweis |
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| GB9725237D0 (en) | 1997-11-29 | 1998-01-28 | Secr Defence | Amplification system |
| GB9803382D0 (en) * | 1998-02-19 | 1998-04-15 | Secr Defence | Detection system |
| GB9812768D0 (en) * | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
| US6440675B1 (en) * | 1999-09-17 | 2002-08-27 | Whitehead Institute For Biomedical Research | Methods for selecting primers |
-
1999
- 1999-08-04 GB GBGB9918237.0A patent/GB9918237D0/en not_active Ceased
-
2000
- 2000-08-03 DE DE60017750T patent/DE60017750T2/de not_active Expired - Lifetime
- 2000-08-03 AU AU64558/00A patent/AU774766B2/en not_active Ceased
- 2000-08-03 WO PCT/GB2000/003016 patent/WO2001011078A1/en not_active Ceased
- 2000-08-03 JP JP2001515326A patent/JP4628625B2/ja not_active Expired - Fee Related
- 2000-08-03 EP EP00951700A patent/EP1198593B1/de not_active Expired - Lifetime
- 2000-08-03 ES ES00951700T patent/ES2245315T3/es not_active Expired - Lifetime
- 2000-08-03 CA CA2381037A patent/CA2381037C/en not_active Expired - Fee Related
- 2000-08-03 AT AT00951700T patent/ATE287969T1/de not_active IP Right Cessation
- 2000-08-03 US US10/048,752 patent/US7015018B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1198593B1 (de) | 2005-01-26 |
| WO2001011078A8 (en) | 2001-06-14 |
| GB9918237D0 (en) | 1999-10-06 |
| DE60017750D1 (de) | 2005-03-03 |
| AU6455800A (en) | 2001-03-05 |
| CA2381037C (en) | 2011-04-12 |
| JP2003506068A (ja) | 2003-02-18 |
| ES2245315T3 (es) | 2006-01-01 |
| US7015018B1 (en) | 2006-03-21 |
| AU774766B2 (en) | 2004-07-08 |
| JP4628625B2 (ja) | 2011-02-09 |
| DE60017750T2 (de) | 2005-12-29 |
| CA2381037A1 (en) | 2001-02-15 |
| WO2001011078A1 (en) | 2001-02-15 |
| EP1198593A1 (de) | 2002-04-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |