WO2008102606A1 - ゲノムdna断片の増幅または欠失の検出方法 - Google Patents

ゲノムdna断片の増幅または欠失の検出方法 Download PDF

Info

Publication number
WO2008102606A1
WO2008102606A1 PCT/JP2008/051215 JP2008051215W WO2008102606A1 WO 2008102606 A1 WO2008102606 A1 WO 2008102606A1 JP 2008051215 W JP2008051215 W JP 2008051215W WO 2008102606 A1 WO2008102606 A1 WO 2008102606A1
Authority
WO
WIPO (PCT)
Prior art keywords
genomic dna
dna fragment
fragment
general formula
labeled
Prior art date
Application number
PCT/JP2008/051215
Other languages
English (en)
French (fr)
Inventor
Makoto Amano
Naoyuki Yamamoto
Original Assignee
Wako Pure Chemical Industries, Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wako Pure Chemical Industries, Ltd. filed Critical Wako Pure Chemical Industries, Ltd.
Priority to US12/524,267 priority Critical patent/US8105770B2/en
Priority to AT08704024T priority patent/ATE553221T1/de
Priority to JP2009500120A priority patent/JP5476989B2/ja
Priority to KR1020097017326A priority patent/KR101444712B1/ko
Priority to EP08704024A priority patent/EP2119797B1/en
Publication of WO2008102606A1 publication Critical patent/WO2008102606A1/ja

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/107—Reactions characterised by their quantitative nature the purpose being quantitative analysis with a competitive internal standard/control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/501—Detection characterised by immobilisation to a surface being an array of oligonucleotides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

課題 　本発明は、より精度良く且つ高感度に検出し得るＣＧＨ法、特にＣＧＨマイクロアレイ法を提供することを課題とする。　 解決手段　（ａ）検査対象となる細胞由来のゲノムＤＮＡ断片である被検ゲノムＤＮＡ断片を下記一般式（１）で示される標識物質または一般式（２）で示される標識物質の何れか一方の標識物質で標識し、当該被検ゲノムＤＮＡ断片との違いを検出するための基準となる対照ゲノム断片を残りの一方の標識物質で標識し、（ｂ）標識された被検ゲノムＤＮＡ断片と標識された対照ゲノムＤＮＡ断片を、被検ゲノム断片と対照ゲノム断片との違いを検出するための核酸配列を含む標本核酸に競合的にハイブリダイズさせ、（ｃ）得られる蛍光強度を指標として、被検ゲノムＤＮＡ断片中の増幅または欠失を検出する方法、並びに下記一般式（１）で示される標識物質で標識されたぬくレオチド残基と下記一般式（２）で示される標識物質で標識されたヌクレオチド残基とを含んでなる、当該方法のためのキット。
PCT/JP2008/051215 2007-01-31 2008-01-28 ゲノムdna断片の増幅または欠失の検出方法 WO2008102606A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US12/524,267 US8105770B2 (en) 2007-01-31 2008-01-28 Method of detecting amplification or deletion in genomic DNA Fragment
AT08704024T ATE553221T1 (de) 2007-01-31 2008-01-28 Verfahren zum nachweis von amplifikation oder deletion in einem genomischen dna-fragment
JP2009500120A JP5476989B2 (ja) 2007-01-31 2008-01-28 ゲノムｄｎａ断片の増幅または欠失の検出方法
KR1020097017326A KR101444712B1 (ko) 2007-01-31 2008-01-28 게놈 ｄｎａ 단편의 증폭 또는 결실의 검출방법
EP08704024A EP2119797B1 (en) 2007-01-31 2008-01-28 Method of detecting amplification or deletion in genomic dna fragment

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007020701 2007-01-31
JP2007-020701 2007-01-31

Publications (1)

Publication Number Publication Date
WO2008102606A1 true WO2008102606A1 (ja) 2008-08-28

Family

ID=39709885

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/051215 WO2008102606A1 (ja) 2007-01-31 2008-01-28 ゲノムdna断片の増幅または欠失の検出方法

Country Status (6)

Country Link
US (1) US8105770B2 (ja)
EP (1) EP2119797B1 (ja)
JP (1) JP5476989B2 (ja)
KR (1) KR101444712B1 (ja)
AT (1) ATE553221T1 (ja)
WO (1) WO2008102606A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024044792A1 (en) 2022-08-26 2024-02-29 Am Chemicals Llc Universal solid supports and phosphoramidite building blocks for oligonucleotide synthesis

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022191485A1 (ko) * 2021-03-10 2022-09-15 에스에프씨 주식회사 리포터 및 이의 용도

Citations (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993018186A1 (en) 1992-03-04 1993-09-16 The Regents Of The University Of California Comparative genomic hybridization (cgh)
WO1996017628A1 (de) 1994-12-07 1996-06-13 Institut für Diagnostikforschung GmbH an der Freien Universität Berlin Verfahren zur in-vivo-diagnostik mittels nir-strahlung
JP2595201B2 (ja) 1983-04-13 1997-04-02 エンゾー バイオケム，インコーポレイティド Ｄｎａ分子もしくはプローブおよび該ｄｎａ分子もしくはプローブの調製方法
WO1999012544A1 (en) 1997-09-11 1999-03-18 Nastech Pharmaceutical Company, Inc. Intranasal formulation containing scopolamine and method of treating motion sickness
JPH1180189A (ja) 1997-07-07 1999-03-26 Rikagaku Kenkyusho ３’−デオキシリボヌクレオチド誘導体
JPH11258233A (ja) 1998-03-13 1999-09-24 Toyobo Co Ltd 癌の悪性度の判定方法およびそのための装置
JP2002193991A (ja) 2000-12-26 2002-07-10 Wako Pure Chem Ind Ltd 修飾ヌクレオチド
JP2005500023A (ja) 2001-05-10 2005-01-06 ベイラー　カレッジ　オブ　メディスン 生体分子のアレイ型ゲノム核酸解析を行うための組成物および方法
JP2005000023A (ja) 2003-06-10 2005-01-06 Takakita Co Ltd 穀実圧砕装置を備えたホールクロップ収穫機
JP2005525786A (ja) 2001-09-27 2005-09-02 スペクトラル ジェノミクス、インク． アレイを用いた遺伝子モザイクの検出方法
JP2005304481A (ja) 2004-02-13 2005-11-04 Joji Inasawa ゲノムｄｎａの定着基盤と当該基盤を用いた染色体異常並びにそれに起因する疾患の検出方法
JP2005304497A (ja) 2004-03-25 2005-11-04 Joji Inasawa 特定の癌関連遺伝子を用いる癌の検出方法及び癌の抑制方法
US6974873B2 (en) 2000-09-29 2005-12-13 Molecular Probes, Inc. Modified carbocyanine dyes and their conjugates
JP2006094726A (ja) 2004-09-28 2006-04-13 National Institute Of Advanced Industrial & Technology 肝細胞癌の悪性度評価方法
JP2006115844A (ja) 2004-08-23 2006-05-11 Macrogen Inc 染色体異常検定方法及びマイクロアレイチップ

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2004286845A1 (en) * 2003-10-30 2005-05-19 Tufts-New England Medical Center Prenatal diagnosis using cell-free fetal DNA in amniotic fluid

Patent Citations (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2595201B2 (ja) 1983-04-13 1997-04-02 エンゾー バイオケム，インコーポレイティド Ｄｎａ分子もしくはプローブおよび該ｄｎａ分子もしくはプローブの調製方法
JPH07505053A (ja) 1992-03-04 1995-06-08 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 比較ゲノムハイブリダイゼーション（ｃｇｈ）
WO1993018186A1 (en) 1992-03-04 1993-09-16 The Regents Of The University Of California Comparative genomic hybridization (cgh)
WO1996017628A1 (de) 1994-12-07 1996-06-13 Institut für Diagnostikforschung GmbH an der Freien Universität Berlin Verfahren zur in-vivo-diagnostik mittels nir-strahlung
JP2002012782A (ja) 1994-12-07 2002-01-15 Inst Fuer Diagnostikforschung Gmbh An Der Freien Univ Berlin 生体内診断剤に適する化合物
JPH1180189A (ja) 1997-07-07 1999-03-26 Rikagaku Kenkyusho ３’−デオキシリボヌクレオチド誘導体
WO1999012544A1 (en) 1997-09-11 1999-03-18 Nastech Pharmaceutical Company, Inc. Intranasal formulation containing scopolamine and method of treating motion sickness
JPH11258233A (ja) 1998-03-13 1999-09-24 Toyobo Co Ltd 癌の悪性度の判定方法およびそのための装置
US6974873B2 (en) 2000-09-29 2005-12-13 Molecular Probes, Inc. Modified carbocyanine dyes and their conjugates
JP2002193991A (ja) 2000-12-26 2002-07-10 Wako Pure Chem Ind Ltd 修飾ヌクレオチド
JP2005500023A (ja) 2001-05-10 2005-01-06 ベイラー　カレッジ　オブ　メディスン 生体分子のアレイ型ゲノム核酸解析を行うための組成物および方法
JP2005525786A (ja) 2001-09-27 2005-09-02 スペクトラル ジェノミクス、インク． アレイを用いた遺伝子モザイクの検出方法
JP2005000023A (ja) 2003-06-10 2005-01-06 Takakita Co Ltd 穀実圧砕装置を備えたホールクロップ収穫機
JP2005304481A (ja) 2004-02-13 2005-11-04 Joji Inasawa ゲノムｄｎａの定着基盤と当該基盤を用いた染色体異常並びにそれに起因する疾患の検出方法
JP2005304497A (ja) 2004-03-25 2005-11-04 Joji Inasawa 特定の癌関連遺伝子を用いる癌の検出方法及び癌の抑制方法
JP2006115844A (ja) 2004-08-23 2006-05-11 Macrogen Inc 染色体異常検定方法及びマイクロアレイチップ
JP2006094726A (ja) 2004-09-28 2006-04-13 National Institute Of Advanced Industrial & Technology 肝細胞癌の悪性度評価方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GENOMICS, vol. 87, 2006, pages 298 - 306
KALLIONIEMI ET AL., SCIENCE, vol. 258, 1992, pages 818 - 821
NUCLEIC ACIDS RESEARCH, vol. 14, no. 19, 1986, pages 7617

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024044792A1 (en) 2022-08-26 2024-02-29 Am Chemicals Llc Universal solid supports and phosphoramidite building blocks for oligonucleotide synthesis

Also Published As

Publication number Publication date
KR101444712B1 (ko) 2014-09-26
EP2119797B1 (en) 2012-04-11
US8105770B2 (en) 2012-01-31
JP5476989B2 (ja) 2014-04-23
ATE553221T1 (de) 2012-04-15
US20100112711A1 (en) 2010-05-06
JPWO2008102606A1 (ja) 2010-05-27
KR20090106414A (ko) 2009-10-08
EP2119797A4 (en) 2011-04-20
EP2119797A1 (en) 2009-11-18

Similar Documents

Publication Publication Date Title
WO2009022125A8 (en) Identification of nucleic acid sequences
WO2011156434A3 (en) Nucleic acid detection and quantification by post-hybridization labeling and universal encoding
WO2010059731A3 (en) Polynucleotide mapping and sequencing
WO2004072297A3 (en) Terminal-phosphate-labeled nucleotides and methods of use
WO2011146629A3 (en) Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
WO2004063700A3 (en) Multiplex analytical platform using molecular tags
IS8314A (is) Ákvörðun fjölkirnisraðar
RU2011117297A (ru) Праймеры и зонды для обнаружения вируса папилломы человека и последовательностей бета-глобина человека в исследуемых образцах
WO2009011971A3 (en) Primers and probes for the detection of streptococcus pneumoniae
ATE307903T1 (de) Immunologischer nachweis von rna:dna hybriden auf mikroarrays
WO2007022530A3 (en) Quantification of microsphere suspension hybridization and uses thereof
WO2013009084A3 (ko) 평행결합 구조의 이중 혼성화 pna 프로브 시스템을 포함하는 실시간 다중 중합효소 연쇄반응에 의한 결핵균 및 비결핵 항산균의 동시 검출용 조성물 및 이를 이용한 검출 방법
WO2007087336A3 (en) Methods for detecting nucleic acids using multiple signals
EP1829964A4 (en) METHOD OF STUDYING A SEQUENCE OF A GENE
WO2009057829A3 (en) Nucleic acid primer set for detection of drug-resistant strain of hepatitis b virus, assay kit, and method of detecting drug-resistant strain of hepatitis b virus
WO2011148269A3 (en) Differentiation of species within the mycobacterium tuberculosis complex
WO2010060046A3 (en) Dye probe fluorescence resonance energy transfer genotyping
CN103045717A (zh) 焦磷酸测序技术检测结核分枝杆菌利福平耐药性的方法
MY149655A (en) Detection and enumeration of microorganisms
WO2008102606A1 (ja) ゲノムdna断片の増幅または欠失の検出方法
WO2004046344A3 (en) Polynucleotide sequence detection assays
CN103045716B (zh) 焦磷酸测序技术检测结核分枝杆菌异烟肼耐药性的方法
RU2011132045A (ru) Праймеры и зонды для детектирования вируса гепатита с
CN103757094B (zh) 一种基于荧光pcr检测tyms基因多态性的方法
WO2012050353A3 (ko) 표적 폴리뉴클레오티드의 염기서열을 비특이적으로 연장하는 방법

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08704024

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase

Ref document number: 2009500120

Country of ref document: JP

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 12524267

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2008704024

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 1020097017326

Country of ref document: KR