DE69828908D1 - Fluorimetrisches system zum nachweis von nucleinsäuren - Google Patents

Fluorimetrisches system zum nachweis von nucleinsäuren

Info

Publication number
DE69828908D1
DE69828908D1 DE69828908T DE69828908T DE69828908D1 DE 69828908 D1 DE69828908 D1 DE 69828908D1 DE 69828908 T DE69828908 T DE 69828908T DE 69828908 T DE69828908 T DE 69828908T DE 69828908 D1 DE69828908 D1 DE 69828908D1
Authority
DE
Germany
Prior art keywords
sequence
probe
sample
fluorescence
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69828908T
Other languages
English (en)
Other versions
DE69828908T2 (de
Inventor
Alan Lee
Roderick Fuerst
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UK Secretary of State for Defence
Original Assignee
UK Secretary of State for Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UK Secretary of State for Defence filed Critical UK Secretary of State for Defence
Publication of DE69828908D1 publication Critical patent/DE69828908D1/de
Application granted granted Critical
Publication of DE69828908T2 publication Critical patent/DE69828908T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6823Release of bound markers
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00Chemistry: analytical and immunological testing
    • Y10S436/80Fluorescent dyes, e.g. rhodamine
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/14Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Eye Examination Apparatus (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Geophysics And Detection Of Objects (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE69828908T 1997-11-29 1998-11-27 Fluorimetrisches system zum nachweis von nucleinsäuren Expired - Lifetime DE69828908T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9725197 1997-11-29
GBGB9725197.9A GB9725197D0 (en) 1997-11-29 1997-11-29 Detection system
PCT/GB1998/003560 WO1999028500A1 (en) 1997-11-29 1998-11-27 Fluorimetric detection system of a nucleic acid

Publications (2)

Publication Number Publication Date
DE69828908D1 true DE69828908D1 (de) 2005-03-10
DE69828908T2 DE69828908T2 (de) 2006-01-05

Family

ID=10822794

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69828908T Expired - Lifetime DE69828908T2 (de) 1997-11-29 1998-11-27 Fluorimetrisches system zum nachweis von nucleinsäuren
DE69841281T Expired - Lifetime DE69841281D1 (de) 1997-11-29 1998-11-27 Fluorimetrisches System zum Nachweis von Nukleinsäuren

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69841281T Expired - Lifetime DE69841281D1 (de) 1997-11-29 1998-11-27 Fluorimetrisches System zum Nachweis von Nukleinsäuren

Country Status (13)

Country Link
US (2) US6833257B2 (de)
EP (2) EP1489190B1 (de)
JP (2) JP4540844B2 (de)
KR (1) KR100641595B1 (de)
AT (2) ATE288500T1 (de)
AU (1) AU743543B2 (de)
CA (1) CA2311952C (de)
DE (2) DE69828908T2 (de)
ES (1) ES2232972T3 (de)
GB (3) GB9725197D0 (de)
NZ (1) NZ504818A (de)
PT (1) PT1049802E (de)
WO (1) WO1999028500A1 (de)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
GB9725197D0 (en) * 1997-11-29 1998-01-28 Secr Defence Detection system
GB9821989D0 (en) 1998-10-08 1998-12-02 Hybaid Ltd Detection of nucleic acid polymorphism
US6403313B1 (en) * 1999-12-21 2002-06-11 Ingeneus Corporation Fluorescent intensity assay for duplex and triplex nucleic acid hybridization solution utilizing fluorescent intercalators
US6858390B2 (en) 1998-12-31 2005-02-22 Ingeneus Corporation Aptamers containing sequences of nucleic acid or nucleic acid analogues bound homologously, or in novel complexes
US6420115B1 (en) 1999-12-21 2002-07-16 Ingeneus Corporation Cation mediated triplex hybridization assay
US6900300B1 (en) 2000-09-19 2005-05-31 Ingeneus Corporation Quadruplex DNA and duplex probe systems
US6656692B2 (en) 1999-12-21 2003-12-02 Ingeneus Corporation Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US6197520B1 (en) * 1999-08-13 2001-03-06 University Of Utah Research Foundation Solution-based color compensation adjusted for temperature and electronic gains
GB9928232D0 (en) * 1999-12-01 2000-01-26 Skelton Stephen Detection system
US6927027B2 (en) 1999-12-21 2005-08-09 Ingeneus Corporation Nucleic acid multiplex formation
US6911536B1 (en) 1999-12-21 2005-06-28 Ingeneus Corporation Triplex and quadruplex catalytic hybridization
US6924108B2 (en) 1999-12-21 2005-08-02 Ingeneus Corporation Nucleic acid binding enhancement by conjugation with nucleotides, nucleosides, bases and/or their analogues
US7309569B2 (en) 1999-12-21 2007-12-18 Ingeneus, Inc. Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US7052844B2 (en) 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
US7220541B2 (en) 2000-01-24 2007-05-22 Ingeneus, Inc. Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions
US6982147B2 (en) 2000-01-24 2006-01-03 Ingeneus Corporation Apparatus for assaying biopolymer binding by means of multiple measurements under varied conditions
US6613524B1 (en) 2000-01-24 2003-09-02 Ingeneus Corporation Amperometric affinity assay and electrically stimulated complexes of nucleic acids
GB0005281D0 (en) 2000-03-07 2000-04-26 Secr Defence Analytical method
GB2360088A (en) * 2000-03-07 2001-09-12 Secr Defence Method and kit for determining PCR amplification reaction conditions
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
US6977161B2 (en) 2000-10-14 2005-12-20 Eragen Biosciences, Inc. Solid support assay systems and methods utilizing non-standard bases
CN1592792B (zh) * 2000-05-19 2010-12-01 伊拉根生物科学公司 检测核酸的材料和方法
GB0110501D0 (en) 2001-04-30 2001-06-20 Secr Defence Brit Amplification process
GB0111275D0 (en) * 2001-05-09 2001-06-27 Secr Defence Analytical method and kit
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
FI114399B (fi) * 2001-06-06 2004-10-15 Biohit Oyj Menetelmä ekstensiotuotteiden määrittämiseksi
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US8110351B2 (en) 2002-01-16 2012-02-07 Invitrogen Dynal As Method for isolating nucleic acids and protein from a single sample
KR100464084B1 (ko) * 2002-01-17 2005-01-03 (주)지노첵 다기능성 프라이머를 이용하여 중합효소연쇄반응의 산물을확인하는 방법
JPWO2003062418A1 (ja) * 2002-01-25 2005-05-19 オリンパス株式会社 核酸情報の検出方法及び装置
GB0205455D0 (en) * 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US7166478B2 (en) 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
DE60317420T2 (de) 2002-09-02 2008-09-18 Toyo Boseki K.K. Verfahren zu Identifizierung von Nukleotid-Polymorphismen unter Verwendung von Resonanzenergietransfer
GB0223563D0 (en) * 2002-10-10 2002-11-20 Secr Defence Detection system
WO2004038038A2 (en) * 2002-10-23 2004-05-06 University Of Utah Research Foundation Amplicon melting analysis with saturation dyes
GB0229287D0 (en) * 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
CA2513626C (en) * 2003-01-17 2013-03-19 Eragen Biosciences, Inc. Nucleic acid amplification using non-standard bases
GB0304832D0 (en) 2003-03-04 2003-04-09 Secr Defence Assay method
JP4646904B2 (ja) * 2003-03-07 2011-03-09 ユニバーシティー、オブ、メディシン、アンド、デンティストリー、オブ、ニュージャージー 光学的にデコード可能なマイクロキャリア、アレイ及び方法
JP4567673B2 (ja) * 2003-04-01 2010-10-20 エラジェン バイオサイエンシズ インコーポレイテッド ポリメラーゼ阻害剤およびその使用方法
WO2005047470A2 (en) * 2003-11-07 2005-05-26 U.S. Genomics, Inc. Intercalator fret donors or acceptors
KR100906749B1 (ko) * 2004-03-25 2009-07-09 (주)바이오니아 인터컬레이팅 형광염료가 표지된 프로브를 이용한 핵산 증폭 측정 방법
US9657347B2 (en) 2004-04-20 2017-05-23 University of Utah Research Foundation and BioFire Defense, LLC Nucleic acid melting analysis with saturation dyes
US7387887B2 (en) * 2004-04-20 2008-06-17 University Of Utah Research Foundation Nucleic acid melting analysis with saturation dyes
KR101424812B1 (ko) 2004-10-18 2014-08-07 브랜데이스 유니버시티 핵산 증폭을 위한 프라이머, 프로브 및 방법
GB0503172D0 (en) 2005-02-16 2005-03-23 Enigma Diagnostics Ltd Detection method
US7498136B2 (en) * 2005-03-18 2009-03-03 Eragen Biosciences, Inc. Methods for detecting multiple species and subspecies of Neisseria
CA2610863C (en) 2005-06-07 2017-01-17 Eragen Biosciences, Inc. Methods for detection and typing of nucleic acids
US20070072211A1 (en) * 2005-06-30 2007-03-29 Roche Molecular Systems, Inc. Asymmetric PCR coupled with post-PCR characterization for the identification of nucleic acids
GB0517005D0 (en) 2005-08-19 2005-09-28 Enigma Diagnostics Ltd Analytical method and kit
PL1937834T3 (pl) * 2005-09-01 2014-11-28 Mtpcr Pty Ltd Sposoby wzmacniania, kwantyfikacji i identyfikacji kwasów nukleinowych
GB0603190D0 (en) * 2006-02-16 2006-03-29 Enigma Diagnostics Ltd Detection system
US20070264694A1 (en) * 2006-04-07 2007-11-15 Eragen Biosciences, Inc. Use of non-standard bases and proximity effects for gene assembly and conversion of non-standard bases to standard bases during dna synthesis
US8563251B2 (en) * 2006-05-12 2013-10-22 San Diego State University (Sdsu) Foundation High-throughput methods for quantifying cells in environmental and laboratory samples
JP5593582B2 (ja) * 2007-06-12 2014-09-24 東洋紡株式会社 核酸の迅速な検出方法
JP5286995B2 (ja) * 2007-07-13 2013-09-11 東洋紡株式会社 抗酸菌属細菌の検出用オリゴヌクレオチドおよびその用途
JP5286996B2 (ja) * 2007-07-13 2013-09-11 東洋紡株式会社 抗酸菌属細菌の検出用オリゴヌクレオチドおよびその用途
JP5286998B2 (ja) * 2007-07-13 2013-09-11 東洋紡株式会社 抗酸菌属細菌の菌種同定用オリゴヌクレオチドおよびその用途
JP5286997B2 (ja) * 2007-07-13 2013-09-11 東洋紡株式会社 抗酸菌属細菌の検出用オリゴヌクレオチドおよびその用途
JP4557014B2 (ja) * 2008-02-14 2010-10-06 ソニー株式会社 核酸検出用蛍光標識オリゴヌクレオチド、及び該核酸検出用蛍光標識オリゴヌクレオチドを用いた二本鎖形成に関わる情報を得る方法
US9249455B2 (en) * 2008-04-18 2016-02-02 Luminex Corporation Methods for detection and quantification of small RNA
US20100129796A1 (en) * 2008-11-24 2010-05-27 Micah Halpern Dye probe fluorescence resonance energy transfer genotyping
EP2443256A1 (de) 2009-06-15 2012-04-25 BG Research Ltd Nukleinsäurenachweis
GB0915664D0 (en) 2009-09-08 2009-10-07 Enigma Diagnostics Ltd Reaction method
WO2011050173A1 (en) 2009-10-21 2011-04-28 Brandeis University Methods, kits and reaction mixtures for analyzing single-stranded nucleic acid sequences
CA2778449C (en) 2009-10-23 2017-06-20 Luminex Corporation Amplification primers with non-standard bases for increased reaction specificity
EP2524053A1 (de) * 2010-01-15 2012-11-21 Steffen Mergemeier Verfahren zur erkennung mehr als eines ziels in einem pcr-basierten ansatz mit einer unspezifischen und nicht mit der emission fluorphor-etikettierter sonden interferierenden matrize
GB201004339D0 (en) 2010-03-16 2010-04-28 Enigma Diagnostics Ltd Sequence detection assay
GB201007868D0 (en) 2010-05-11 2010-06-23 Enigma Diagnostics Ltd Sequence detection assay
US20140186827A1 (en) * 2010-05-14 2014-07-03 Fluidigm, Inc. Assays for the detection of genotype, mutations, and/or aneuploidy
US9416153B2 (en) 2011-10-11 2016-08-16 Enzo Life Sciences, Inc. Fluorescent dyes
US8956815B2 (en) 2012-04-18 2015-02-17 Toxic Report Llc Intercalation methods and devices
KR101441594B1 (ko) 2012-12-27 2014-09-23 매쓰파워 주식회사 온도 민감 폴리머 합성체를 이용한 핵산 증폭 디스크 장치 및 이를 이용한 분석 방법
US9957393B2 (en) 2015-03-30 2018-05-01 Enzo Biochem, Inc. Monoazo dyes with cyclic amine as fluorescence quenchers
US10907216B2 (en) 2016-05-06 2021-02-02 Public University Corporation Nagoya City University Prediction of hepatocellular carcinoma onset after clearance of hepatitis C virus
KR102237237B1 (ko) * 2017-11-28 2021-04-07 에스에프씨 주식회사 소광자 및 이의 용도
KR102262100B1 (ko) * 2018-12-04 2021-06-08 에스에프씨 주식회사 소광자 및 이의 용도
KR102246542B1 (ko) * 2018-12-04 2021-04-30 에스에프씨 주식회사 소광자 및 이의 용도
CN110499151A (zh) * 2019-08-29 2019-11-26 青岛科技大学 一种树枝状放大的荧光信号探针及其制法和应用

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
US4883750A (en) * 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4868103A (en) * 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
US5015570A (en) * 1988-05-13 1991-05-14 Molecular Therapeutics, Inc. Molecular diagnosis of Alzheimer Disease
WO1990011372A1 (en) * 1989-03-21 1990-10-04 Collaborative Research, Inc. Multiplex dna diagnostic test
EP0553258A4 (en) * 1990-10-17 1993-09-22 Jack D. Love Identification and paternity determination by detecting presence or absence of multiple nucleic acid sequences
IL100908A0 (en) * 1991-02-22 1992-11-15 Salk Inst Biotech Ind Invertase genes and their use
US5512463A (en) * 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5849486A (en) * 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
US5567583A (en) * 1991-12-16 1996-10-22 Biotronics Corporation Methods for reducing non-specific priming in DNA detection
JPH06511387A (ja) * 1991-12-24 1994-12-22 アイシス・ファーマシューティカルス・インコーポレーテッド β−アミロイド調節のための組成物および方法
US5674682A (en) * 1992-10-29 1997-10-07 Thomas Jefferson University Nucleic acid primers for detecting micrometastasis of prostate cancer
AU7662794A (en) 1993-09-23 1995-04-10 Zeneca Limited Nucleic acid detection with energy transfer
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
NO301295B1 (no) * 1994-03-28 1997-10-06 Geberit Technik Ag Stativ med i det minste to på tvers av hverandre forlöpende hulprofilstenger
US5491063A (en) * 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
DE69527125T2 (de) * 1994-11-09 2003-02-27 Hitachi, Ltd. Verfahren und Vorrichtung zur DNS Analyse
US5811239A (en) * 1996-05-13 1998-09-22 Frayne Consultants Method for single base-pair DNA sequence variation detection
EP1736554B1 (de) * 1996-05-29 2013-10-09 Cornell Research Foundation, Inc. Bestimmung von Unterschieden in der Nukleinsäuresequenz mittels einer Kombination von Ligasebestimmung und Polymerasekettenreaktion
CA2658290C (en) * 1996-06-04 2012-04-10 University Of Utah Research Foundation Monitoring hybridization during pcr using fluorescent dye specific to double-stranded dna
WO1998018965A1 (en) * 1996-10-29 1998-05-07 University Of Nebraska At Lincoln Method for detecting point mutations in dna utilizing fluorescence energy transfer
IL131332A (en) * 1997-02-12 2003-07-31 Eugene Y Chan Methods and products for analyzing polymers
US6403311B1 (en) * 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
US5830661A (en) * 1997-02-13 1998-11-03 The University Of Connecticut Diagnosis and treatment of glaucoma
GB9725197D0 (en) * 1997-11-29 1998-01-28 Secr Defence Detection system
GB9803382D0 (en) * 1998-02-19 1998-04-15 Secr Defence Detection system
WO2003046208A2 (en) * 2001-11-28 2003-06-05 Mj Bioworks Incorporated Parallel polymorphism scoring by amplification and error correction
US7919235B2 (en) * 2002-09-30 2011-04-05 F. Hoffman-La Roche Ag Oligonucleotides for genotyping thymidylate synthase gene
US7354706B2 (en) * 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
JP2007512811A (ja) * 2003-11-10 2007-05-24 インベスチゲン, インコーポレイテッド 検出のための核酸を調製する方法
EP2333059A4 (de) * 2008-07-30 2013-01-09 Nippon Steel Kankyo Engineering Co Ltd Universelles nukleinsäuresondenset und anwendungsvefahren dafür

Also Published As

Publication number Publication date
ATE447624T1 (de) 2009-11-15
EP1049802B1 (de) 2005-02-02
DE69841281D1 (de) 2009-12-17
EP1489190A2 (de) 2004-12-22
GB2346972A (en) 2000-08-23
AU743543B2 (en) 2002-01-31
GB9825924D0 (en) 1999-01-20
PT1049802E (pt) 2005-05-31
AU1342599A (en) 1999-06-16
GB2333359A (en) 1999-07-21
KR20010015854A (ko) 2001-02-26
NZ504818A (en) 2002-10-25
ATE288500T1 (de) 2005-02-15
CA2311952C (en) 2009-10-06
WO1999028500A1 (en) 1999-06-10
CA2311952A1 (en) 1999-06-10
GB0012468D0 (en) 2000-07-12
KR100641595B1 (ko) 2006-11-06
GB2346972B (en) 2002-09-04
EP1489190A3 (de) 2006-06-07
JP4540844B2 (ja) 2010-09-08
ES2232972T3 (es) 2005-06-01
JP2003500001A (ja) 2003-01-07
US6833257B2 (en) 2004-12-21
US20050112647A1 (en) 2005-05-26
JP2009195238A (ja) 2009-09-03
DE69828908T2 (de) 2006-01-05
EP1489190B1 (de) 2009-11-04
US20020119450A1 (en) 2002-08-29
EP1049802A1 (de) 2000-11-08
GB9725197D0 (en) 1998-01-28

Similar Documents

Publication Publication Date Title
DE69828908D1 (de) Fluorimetrisches system zum nachweis von nucleinsäuren
CA2321185A1 (en) Method for detection of target nucleic acids using pcr
EP0664339A4 (de) Methode und testsatz zur unterscheidung von dna.
EP1448581A4 (de) Verfahren zum nachweis und zur quantifizierung von analyten in komplexen mischungen
ES543660A0 (es) Un metodo para determinar una secuencia particular de poli- nucleotidos en un medio de ensayo que contiene acidos nu- cleicos monocatenarios
PT1390542E (pt) Metodo de deteccao de acidos nucleicos
WO1991017264A1 (en) Assay for specific nucleic acid sequences
DE59814239D1 (de) Integriertes Verfahren und System zur Amplifizierung und zum Nachweis von Nukleinsäuren
EP1686191A3 (de) Fluorometrisches Verfahren zur Überwachung und zum Nachweis von Nukleinsäure- Amplifizierung
WO2000040751A3 (en) Detecting nucleic acids
EP0843019A3 (de) Verfahren zur Bestimmung einer spezifischen Nukleinsäuresequenz, und ein Reagenz für diesen Zweck
DE60017750D1 (de) Amplifizierungsverfahen zum nachweis von zielnukleinsäuren unter verwendung von fluoreszenzenergieübertragung
WO2001038587A3 (en) Energy-transfer assay for polynucleic acid polymerases
Boulet Application of Capillary Electrophoresis Laser Induced Fluorescence to the Detection of Nucleic Acid Probe Fragments
DE3567908D1 (en) Photoimmune detection of dna and rna

Legal Events

Date Code Title Description
8364 No opposition during term of opposition