DE60017750D1 - Amplifizierungsverfahen zum nachweis von zielnukleinsäuren unter verwendung von fluoreszenzenergieübertragung - Google Patents

Amplifizierungsverfahen zum nachweis von zielnukleinsäuren unter verwendung von fluoreszenzenergieübertragung

Info

Publication number
DE60017750D1
DE60017750D1 DE60017750T DE60017750T DE60017750D1 DE 60017750 D1 DE60017750 D1 DE 60017750D1 DE 60017750 T DE60017750 T DE 60017750T DE 60017750 T DE60017750 T DE 60017750T DE 60017750 D1 DE60017750 D1 DE 60017750D1
Authority
DE
Germany
Prior art keywords
label
target nucleic
sample
sequence
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60017750T
Other languages
English (en)
Other versions
DE60017750T2 (de
Inventor
Alan Lee
Dario Lyall Leslie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UK Secretary of State for Defence
Original Assignee
UK Secretary of State for Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UK Secretary of State for Defence filed Critical UK Secretary of State for Defence
Application granted granted Critical
Publication of DE60017750D1 publication Critical patent/DE60017750D1/de
Publication of DE60017750T2 publication Critical patent/DE60017750T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
DE60017750T 1999-08-04 2000-08-03 Amplifizierungsverfahren zum Nachweis von zu bestimmenden Nucleinsäuren und Anwendung von Flourszenzenergieübertragung Expired - Lifetime DE60017750T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9918237 1999-08-04
GBGB9918237.0A GB9918237D0 (en) 1999-08-04 1999-08-04 Detection system
PCT/GB2000/003016 WO2001011078A1 (en) 1999-08-04 2000-08-03 Amplification method for detection of target nucleic acids involving fluorescence energy transfer

Publications (2)

Publication Number Publication Date
DE60017750D1 true DE60017750D1 (de) 2005-03-03
DE60017750T2 DE60017750T2 (de) 2005-12-29

Family

ID=10858467

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60017750T Expired - Lifetime DE60017750T2 (de) 1999-08-04 2000-08-03 Amplifizierungsverfahren zum Nachweis von zu bestimmenden Nucleinsäuren und Anwendung von Flourszenzenergieübertragung

Country Status (10)

Country Link
US (1) US7015018B1 (de)
EP (1) EP1198593B1 (de)
JP (1) JP4628625B2 (de)
AT (1) ATE287969T1 (de)
AU (1) AU774766B2 (de)
CA (1) CA2381037C (de)
DE (1) DE60017750T2 (de)
ES (1) ES2245315T3 (de)
GB (1) GB9918237D0 (de)
WO (1) WO2001011078A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2443894A1 (en) * 2001-04-11 2002-10-24 Charlie Xiang Modified random primers for probe labeling
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
US20070059690A1 (en) * 2002-05-31 2007-03-15 Amirul Islam "Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands"
US8658366B2 (en) * 2008-09-18 2014-02-25 Roche Molecular Systems, Inc. Detection of target variants using a fluorescent label and a soluble quencher
CN103339256B (zh) 2011-01-26 2016-03-16 奥林巴斯株式会社 鉴别核酸分子多态性的方法
JP5856984B2 (ja) * 2011-01-26 2016-02-10 オリンパス株式会社 核酸分子の多型識別方法
CN112266950A (zh) * 2020-10-23 2021-01-26 深圳澳东检验检测科技有限公司 一种探针引物组合及其检测试剂盒

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69207580T2 (de) 1992-03-23 1996-08-08 Hoffmann La Roche Verfahren zum DNS-Nachweis
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
GB9725237D0 (en) * 1997-11-29 1998-01-28 Secr Defence Amplification system
GB9803382D0 (en) * 1998-02-19 1998-04-15 Secr Defence Detection system
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6440675B1 (en) * 1999-09-17 2002-08-27 Whitehead Institute For Biomedical Research Methods for selecting primers

Also Published As

Publication number Publication date
WO2001011078A8 (en) 2001-06-14
JP2003506068A (ja) 2003-02-18
US7015018B1 (en) 2006-03-21
DE60017750T2 (de) 2005-12-29
CA2381037A1 (en) 2001-02-15
EP1198593B1 (de) 2005-01-26
GB9918237D0 (en) 1999-10-06
JP4628625B2 (ja) 2011-02-09
ES2245315T3 (es) 2006-01-01
WO2001011078A1 (en) 2001-02-15
CA2381037C (en) 2011-04-12
AU774766B2 (en) 2004-07-08
EP1198593A1 (de) 2002-04-24
ATE287969T1 (de) 2005-02-15
AU6455800A (en) 2001-03-05

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Legal Events

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