DE69031665T2 - Nachweis von Nukleinsäuresequenzen unter Verwendung von Fluoreszenz-Polarisation - Google Patents

Nachweis von Nukleinsäuresequenzen unter Verwendung von Fluoreszenz-Polarisation

Info

Publication number
DE69031665T2
DE69031665T2 DE69031665T DE69031665T DE69031665T2 DE 69031665 T2 DE69031665 T2 DE 69031665T2 DE 69031665 T DE69031665 T DE 69031665T DE 69031665 T DE69031665 T DE 69031665T DE 69031665 T2 DE69031665 T2 DE 69031665T2
Authority
DE
Germany
Prior art keywords
nucleic acid
detection
acid sequences
fluorescence polarization
detect
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69031665T
Other languages
English (en)
Other versions
DE69031665D1 (de
Inventor
Andrew John Garman
Robert Stanley Moore
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GARMAN, ANDREW, ASHTON, CHESTER, GB
Original Assignee
Zeneca Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeneca Ltd filed Critical Zeneca Ltd
Publication of DE69031665D1 publication Critical patent/DE69031665D1/de
Application granted granted Critical
Publication of DE69031665T2 publication Critical patent/DE69031665T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
DE69031665T 1989-02-07 1990-02-02 Nachweis von Nukleinsäuresequenzen unter Verwendung von Fluoreszenz-Polarisation Expired - Fee Related DE69031665T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB898902689A GB8902689D0 (en) 1989-02-07 1989-02-07 Assay method

Publications (2)

Publication Number Publication Date
DE69031665D1 DE69031665D1 (de) 1997-12-11
DE69031665T2 true DE69031665T2 (de) 1998-02-26

Family

ID=10651255

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69031665T Expired - Fee Related DE69031665T2 (de) 1989-02-07 1990-02-02 Nachweis von Nukleinsäuresequenzen unter Verwendung von Fluoreszenz-Polarisation

Country Status (14)

Country Link
US (1) US6022686A (de)
EP (1) EP0382433B1 (de)
JP (1) JPH02295496A (de)
AT (1) ATE159984T1 (de)
AU (1) AU4902590A (de)
CA (1) CA2009454C (de)
DE (1) DE69031665T2 (de)
ES (1) ES2109226T3 (de)
FI (1) FI900602A0 (de)
GB (2) GB8902689D0 (de)
IE (1) IE900442L (de)
IL (1) IL93297A0 (de)
NO (1) NO900582L (de)
ZA (1) ZA90912B (de)

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US6180408B1 (en) 1998-08-21 2001-01-30 Washington University Fluorescence polarization in nucleic acid analysis
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US6100039A (en) * 1999-01-28 2000-08-08 Panvera, Inc. Process for reverse transcriptase activity measurement using fluorescence polarization
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AU6152301A (en) * 2000-05-12 2001-11-26 Caliper Techn Corp Detection of nucleic acid hybridization by fluorescence polarization
US7422850B2 (en) 2000-05-19 2008-09-09 Eragen Biosciences, Inc. Materials and methods for detection of nucleic acids
US6258546B1 (en) * 2000-06-23 2001-07-10 Becton, Dickinson And Company Detection of nucleic acid amplification
DE60137649D1 (de) 2000-10-14 2009-03-26 Eragen Biosciences Inc Nachweissysteme auf festen trägern und methoden zur verwendung von nicht-standardbasen
AU2002227982A1 (en) * 2000-12-05 2002-06-18 Syngenta Participations Ag Method and kit for identification of nucleic acid modification enzymes and inhibitors thereof
DE10104937B4 (de) * 2001-01-29 2005-03-17 Epigenomics Ag Fluoreszenzpolarisation 2
DE10104938B4 (de) * 2001-01-29 2005-06-23 Epigenomics Ag Fluoreszenzpolarisation 1
US8148171B2 (en) 2001-10-09 2012-04-03 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US6589744B2 (en) 2001-11-26 2003-07-08 Syngenta Participations Ag Method and kit for identification for nucleic acid modification enzymes and inhibitors thereof
WO2004027384A2 (en) * 2002-09-17 2004-04-01 Perkinelmer Life Sciences, Inc. Real-time detection of nucleic acid reactions
EP1590482B1 (de) 2003-01-17 2008-12-10 Eragen Biosciences, Inc. Nukleinsäureamplifikation mit nichtstandardbasen
CA2520538C (en) 2003-03-31 2014-04-29 F. Hoffmann-La Roche Ag Compositions and methods for detecting certain flaviviruses, including members of the japanese encephalitis virus serogroup
WO2004090153A2 (en) 2003-04-01 2004-10-21 Eragen Biosciences,Inc. Polymerase inhibitor and method of using same
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EP1698897B1 (de) * 2003-12-19 2010-03-17 Nippon Steel Kankyo Engineering Co., Ltd. Neue gemische zum testen von nukleinsäuren, neues verfahren zum testen von nukleinsäuren unter verwendung davon sowie dafür zu verwendende nukleinsäuresonde
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
US7851152B2 (en) * 2004-09-25 2010-12-14 Yaodong Chen Fluorescent base analogues' usage in the characterization of nucleic acid molecules and their interactions
US20060172314A1 (en) * 2005-01-31 2006-08-03 Song Min-Sun Quantification of amplified nucleic acids
US7498136B2 (en) 2005-03-18 2009-03-03 Eragen Biosciences, Inc. Methods for detecting multiple species and subspecies of Neisseria
WO2006112141A1 (ja) * 2005-03-30 2006-10-26 Toyo Boseki Kabushiki Kaisha 塩基判定方法及び塩基判定用キット
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JP2007330134A (ja) * 2006-06-14 2007-12-27 Toyobo Co Ltd 核酸の検出方法
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
US20110059431A1 (en) * 2007-06-18 2011-03-10 Northwestern University Non-enzymatic detection of bacterial genomic dna
WO2010112033A2 (en) 2009-03-31 2010-10-07 Østjysk Innovation A/S Method for estimating the risk of having or developing multiple sclerosis using sequence polymorphisms in a specific region of chromosome x
JP5938348B2 (ja) 2009-10-23 2016-06-22 ルミネックス コーポレーション 反応特異性の増加のための非標準塩基を含む増幅プライマー
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US20140213471A1 (en) * 2013-01-24 2014-07-31 California Institute Of Technology Chromophore-based characterization and detection methods

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Also Published As

Publication number Publication date
CA2009454A1 (en) 1990-08-07
ATE159984T1 (de) 1997-11-15
DE69031665D1 (de) 1997-12-11
EP0382433A2 (de) 1990-08-16
GB9002536D0 (en) 1990-04-04
FI900602A0 (fi) 1990-02-07
GB2228998B (en) 1993-04-07
CA2009454C (en) 2000-04-04
EP0382433B1 (de) 1997-11-05
IE900442L (en) 1990-08-07
NO900582L (no) 1990-08-08
ES2109226T3 (es) 1998-01-16
US6022686A (en) 2000-02-08
GB2228998A (en) 1990-09-12
GB8902689D0 (en) 1989-03-30
AU4902590A (en) 1990-08-16
IL93297A0 (en) 1990-11-29
JPH02295496A (ja) 1990-12-06
NO900582D0 (no) 1990-02-07
EP0382433A3 (de) 1992-01-15
ZA90912B (en) 1990-10-31

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: ASTRAZENECA UK LTD., LONDON, GB

8327 Change in the person/name/address of the patent owner

Owner name: GARMAN, ANDREW, ASHTON, CHESTER, GB

8339 Ceased/non-payment of the annual fee