ES2109226T3 - Deteccion de secuencias de acidos nucleicos usando polarizacion de fluorescencia. - Google Patents
Deteccion de secuencias de acidos nucleicos usando polarizacion de fluorescencia.Info
- Publication number
- ES2109226T3 ES2109226T3 ES90301135T ES90301135T ES2109226T3 ES 2109226 T3 ES2109226 T3 ES 2109226T3 ES 90301135 T ES90301135 T ES 90301135T ES 90301135 T ES90301135 T ES 90301135T ES 2109226 T3 ES2109226 T3 ES 2109226T3
- Authority
- ES
- Spain
- Prior art keywords
- nucleic acid
- detection
- acid sequences
- fluorescence polarization
- detect
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Hybrid Electric Vehicles (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
- Surgical Instruments (AREA)
- Transducers For Ultrasonic Waves (AREA)
Abstract
METODO DE ENSAYO PARA LA DETECCION DE SECUENCIAS DE ACIDO NUCLEICO EN SOLUCIONES HOMOGENEAS. EL METODO COMPRENDE EL USO DE POLARIZACION FLUORESCENTE PARA DETECTAR LA HIBRIDACION DE UNA MUESTRA DE ACIDO NUCLEICO FLUORESCENTE O PARA DETECTAR PRODUCTOS DE PRIMERA EXTENSION FLUORESCENTE. EQUIPO DE ENSAYO PARA USAR EN EL METODO ANTERIOR.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB898902689A GB8902689D0 (en) | 1989-02-07 | 1989-02-07 | Assay method |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2109226T3 true ES2109226T3 (es) | 1998-01-16 |
Family
ID=10651255
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES90301135T Expired - Lifetime ES2109226T3 (es) | 1989-02-07 | 1990-02-02 | Deteccion de secuencias de acidos nucleicos usando polarizacion de fluorescencia. |
Country Status (14)
Country | Link |
---|---|
US (1) | US6022686A (es) |
EP (1) | EP0382433B1 (es) |
JP (1) | JPH02295496A (es) |
AT (1) | ATE159984T1 (es) |
AU (1) | AU4902590A (es) |
CA (1) | CA2009454C (es) |
DE (1) | DE69031665T2 (es) |
ES (1) | ES2109226T3 (es) |
FI (1) | FI900602A0 (es) |
GB (2) | GB8902689D0 (es) |
IE (1) | IE900442L (es) |
IL (1) | IL93297A0 (es) |
NO (1) | NO900582L (es) |
ZA (1) | ZA90912B (es) |
Families Citing this family (67)
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US6007984A (en) * | 1989-11-01 | 1999-12-28 | Zeneca Limited | Detection of DNA/RNA by fluorescence polarization |
US5453355A (en) * | 1990-01-26 | 1995-09-26 | Abbott Laboratories | Oligonucleotides and methods for the detection of Neisseria gonorrhoeae |
US5641629A (en) * | 1990-06-11 | 1997-06-24 | Nexstar Pharmacueticals Inc | Spectroscopically detectable nucleic acid ligands |
ATE148503T1 (de) * | 1990-09-20 | 1997-02-15 | Amoco Corp | Sonde zusammensetzungen für chromosom identifizierung und verfahren |
US5620847A (en) * | 1990-10-05 | 1997-04-15 | Hoffman-La Roche Inc. | Methods and reagents for detection of bacteria in cerebrospinal fluid |
CA2084405A1 (en) * | 1991-04-11 | 1992-10-12 | Chao-Huei Jeffrey Wang | Detection of dna/rna by fluorescence polarization |
GB9119735D0 (en) * | 1991-09-16 | 1991-10-30 | Secr Defence | Gene probe biosensor method |
US6033854A (en) * | 1991-12-16 | 2000-03-07 | Biotronics Corporation | Quantitative PCR using blocking oligonucleotides |
US5348853A (en) * | 1991-12-16 | 1994-09-20 | Biotronics Corporation | Method for reducing non-specific priming in DNA amplification |
US5567583A (en) * | 1991-12-16 | 1996-10-22 | Biotronics Corporation | Methods for reducing non-specific priming in DNA detection |
US5445935A (en) * | 1992-11-23 | 1995-08-29 | Royer; Catherine A. | Quantitative detection of macromolecules with fluorescent oligonucleotides |
EP0599337B1 (en) * | 1992-11-27 | 2006-03-08 | Canon Kabushiki Kaisha | Method for detection of nucleic acid and probe therefor |
JPH07177899A (ja) * | 1993-12-24 | 1995-07-18 | Bio Sensor Kenkyusho:Kk | 遺伝子の検出方法 |
JPH07233065A (ja) * | 1993-12-27 | 1995-09-05 | Canon Inc | ピリリウム塩又はピリリウム類似塩を含む光化学治療薬 |
US5547861A (en) * | 1994-04-18 | 1996-08-20 | Becton, Dickinson And Company | Detection of nucleic acid amplification |
CA2145719C (en) * | 1994-04-18 | 1998-06-30 | James G. Nadeau | Detection of nucleic acid amplification |
DE69532255D1 (de) * | 1994-05-26 | 2004-01-22 | Canon Kk | Verfahren zum Nachweis von einer Zielsubstanz in einer Probe mit Hilfe von Pyryliumverbindung |
AU705637B2 (en) * | 1994-09-23 | 1999-05-27 | Becton Dickinson & Company | Detection of nucleic acid amplification |
GB9510262D0 (en) * | 1995-05-22 | 1995-07-19 | Nat Blood Authority The | Detection method |
MX9703495A (es) * | 1995-09-12 | 1997-07-31 | Becton Dickinson Co | Dispositivo y metodo para amplificacion y ensayo de adn. |
EP0852004B1 (en) | 1995-10-11 | 2011-01-19 | Luminex Corporation | Multiplexed analysis of clinical specimens |
US5981180A (en) * | 1995-10-11 | 1999-11-09 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and methods |
US5641633A (en) * | 1995-11-15 | 1997-06-24 | Becton, Dickinson And Company | Fluorescence polarization detection of nucleic acids |
US5800989A (en) * | 1995-11-15 | 1998-09-01 | Becton, Dickinson And Company | Method for detection of nucleic acid targets by amplification and fluorescence polarization |
CA2190430A1 (en) * | 1995-12-12 | 1997-06-13 | Margret Barbara Basinski | Method for measuring genetic messages |
US5723294A (en) * | 1996-03-05 | 1998-03-03 | Gull Laboratories | Methods for detection and discrimination of multiple analytes using fluorescent technology |
DE69718268D1 (de) | 1996-10-03 | 2003-02-13 | Canon Kk | Verfahren zur Detektion von Zielnukleinsäure, Verfahren zu ihrer Quantifizierung und Pyrylium-Verbindungen zur Chemilumineszenz-Analyse |
US6449562B1 (en) | 1996-10-10 | 2002-09-10 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and method |
US6503709B1 (en) | 1997-07-03 | 2003-01-07 | Id Biomedical Corporation | Methods for rapidly detecting methicillin resistant staphylococci |
US6261781B1 (en) | 1997-08-05 | 2001-07-17 | Wisconsin Alumni Research Foundation | Direct detection and mutation analysis of low copy number nucleic acids |
US6013442A (en) * | 1997-08-05 | 2000-01-11 | Wisconsin Alumni Res Found | Direct quantitation of low copy number RNA |
US7745142B2 (en) | 1997-09-15 | 2010-06-29 | Molecular Devices Corporation | Molecular modification assays |
US6289229B1 (en) | 1998-01-20 | 2001-09-11 | Scimed Life Systems, Inc. | Readable probe array for in vivo use |
WO1999060158A1 (fr) * | 1998-05-19 | 1999-11-25 | Laboratory Of Molecular Biophotonics | Phase solide utilisee pour detecter de l'acide nucleique et procede de detection d'acide nucleique |
JP3437094B2 (ja) * | 1998-07-03 | 2003-08-18 | 松下電器産業株式会社 | 多波長蛍光偏光法 |
US6180408B1 (en) | 1998-08-21 | 2001-01-30 | Washington University | Fluorescence polarization in nucleic acid analysis |
FR2784748A1 (fr) * | 1998-10-19 | 2000-04-21 | Revelgenics | Detection de reactions de polymerisation par polarisation de fluorescence |
US6100039A (en) * | 1999-01-28 | 2000-08-08 | Panvera, Inc. | Process for reverse transcriptase activity measurement using fluorescence polarization |
CA2366289A1 (en) * | 1999-03-25 | 2000-09-28 | Radoje T. Drmanac | Solution-based methods and materials for sequence analysis by hybridization |
US6777184B2 (en) * | 2000-05-12 | 2004-08-17 | Caliper Life Sciences, Inc. | Detection of nucleic acid hybridization by fluorescence polarization |
CN1592792B (zh) | 2000-05-19 | 2010-12-01 | 伊拉根生物科学公司 | 检测核酸的材料和方法 |
US6258546B1 (en) * | 2000-06-23 | 2001-07-10 | Becton, Dickinson And Company | Detection of nucleic acid amplification |
AU2002213175B2 (en) | 2000-10-14 | 2007-09-13 | Luminex Corporation | Solid support assay systems and methods utilizing non-standard bases |
WO2002046453A2 (en) * | 2000-12-05 | 2002-06-13 | Syngenta Participations Ag | Method and kit for identification of nucleic acid modification enzymes and inhibitors thereof |
DE10104937B4 (de) * | 2001-01-29 | 2005-03-17 | Epigenomics Ag | Fluoreszenzpolarisation 2 |
DE10104938B4 (de) * | 2001-01-29 | 2005-06-23 | Epigenomics Ag | Fluoreszenzpolarisation 1 |
US8148171B2 (en) | 2001-10-09 | 2012-04-03 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and methods |
US6589744B2 (en) | 2001-11-26 | 2003-07-08 | Syngenta Participations Ag | Method and kit for identification for nucleic acid modification enzymes and inhibitors thereof |
CA2498713A1 (en) * | 2002-09-17 | 2004-04-01 | Richard Abraham Joseph | Real-time detection of nucleic acid reactions |
CA2513626C (en) | 2003-01-17 | 2013-03-19 | Eragen Biosciences, Inc. | Nucleic acid amplification using non-standard bases |
JP4782668B2 (ja) | 2003-03-31 | 2011-09-28 | エフ.ホフマン−ラ ロシュ アーゲー | 日本脳炎ウィルス血清グループのメンバーを包含する一定のフラビウィルスを検出するための組成物及び方法 |
CA2521127C (en) | 2003-04-01 | 2014-05-27 | Eragen Biosciences, Inc. | Polymerase inhibitor and method of using same |
GB0317592D0 (en) | 2003-07-26 | 2003-08-27 | Astrazeneca Ab | Method |
WO2005059548A1 (ja) | 2003-12-19 | 2005-06-30 | Kankyo Engineering Co., Ltd. | 核酸測定用新規混合物、及びそれを用いる核酸の新規測定方法並びにそれらに用いる核酸プローブ |
US7939251B2 (en) | 2004-05-06 | 2011-05-10 | Roche Molecular Systems, Inc. | SENP1 as a marker for cancer |
US7851152B2 (en) * | 2004-09-25 | 2010-12-14 | Yaodong Chen | Fluorescent base analogues' usage in the characterization of nucleic acid molecules and their interactions |
US20060172314A1 (en) * | 2005-01-31 | 2006-08-03 | Song Min-Sun | Quantification of amplified nucleic acids |
US7498136B2 (en) | 2005-03-18 | 2009-03-03 | Eragen Biosciences, Inc. | Methods for detecting multiple species and subspecies of Neisseria |
JP4650420B2 (ja) * | 2005-03-30 | 2011-03-16 | 東洋紡績株式会社 | 塩基判定方法及び塩基判定用キット |
EP2489746B1 (en) | 2005-06-07 | 2016-02-03 | Luminex Corporation | Methods for detection and typing of nucleic acids |
JP2007330134A (ja) * | 2006-06-14 | 2007-12-27 | Toyobo Co Ltd | 核酸の検出方法 |
US9938641B2 (en) * | 2006-12-18 | 2018-04-10 | Fluidigm Corporation | Selection of aptamers based on geometry |
WO2008157649A1 (en) * | 2007-06-18 | 2008-12-24 | Northwestern University | Non-enzymatic detection of bacterial genomic dna |
WO2010112033A2 (en) | 2009-03-31 | 2010-10-07 | Østjysk Innovation A/S | Method for estimating the risk of having or developing multiple sclerosis using sequence polymorphisms in a specific region of chromosome x |
CN102639712A (zh) | 2009-10-23 | 2012-08-15 | 卢米耐克斯公司 | 用于提高反应特异性的具有非标准碱基的扩增引物 |
WO2012052758A1 (en) | 2010-10-22 | 2012-04-26 | Astrazeneca Ab | Response biomarkers for iap antagonists in human cancers |
CA2897474A1 (en) | 2013-01-24 | 2014-07-31 | California Institute Of Technology | Chromophore-based characterization and detection methods |
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CA1317535C (en) * | 1987-06-30 | 1993-05-11 | Nanibhushan Dattagupta | Assay of sequences using amplified genes |
US5185439A (en) * | 1987-10-05 | 1993-02-09 | Gen-Probe Incorporated | Acridinium ester labelling and purification of nucleotide probes |
PT88665B (pt) * | 1987-10-05 | 1992-12-31 | Ml Tecnology Ventures Lp | Metodo para a marcacao com ester de acridinio e purificacao de sondas nucleotidicas |
AU622426B2 (en) * | 1987-12-11 | 1992-04-09 | Abbott Laboratories | Assay using template-dependent nucleic acid probe reorganization |
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-
1989
- 1989-02-07 GB GB898902689A patent/GB8902689D0/en active Pending
-
1990
- 1990-02-01 AU AU49025/90A patent/AU4902590A/en not_active Abandoned
- 1990-02-02 EP EP90301135A patent/EP0382433B1/en not_active Expired - Lifetime
- 1990-02-02 DE DE69031665T patent/DE69031665T2/de not_active Expired - Fee Related
- 1990-02-02 AT AT90301135T patent/ATE159984T1/de not_active IP Right Cessation
- 1990-02-02 ES ES90301135T patent/ES2109226T3/es not_active Expired - Lifetime
- 1990-02-05 GB GB9002536A patent/GB2228998B/en not_active Revoked
- 1990-02-07 CA CA002009454A patent/CA2009454C/en not_active Expired - Lifetime
- 1990-02-07 FI FI900602A patent/FI900602A0/fi not_active Application Discontinuation
- 1990-02-07 ZA ZA90912A patent/ZA90912B/xx unknown
- 1990-02-07 JP JP2028127A patent/JPH02295496A/ja active Pending
- 1990-02-07 IL IL93297A patent/IL93297A0/xx unknown
- 1990-02-07 IE IE900442A patent/IE900442L/xx unknown
- 1990-02-07 NO NO90900582A patent/NO900582L/no unknown
-
1993
- 1993-08-03 US US08/101,105 patent/US6022686A/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CA2009454A1 (en) | 1990-08-07 |
IL93297A0 (en) | 1990-11-29 |
GB8902689D0 (en) | 1989-03-30 |
EP0382433B1 (en) | 1997-11-05 |
US6022686A (en) | 2000-02-08 |
DE69031665D1 (de) | 1997-12-11 |
JPH02295496A (ja) | 1990-12-06 |
DE69031665T2 (de) | 1998-02-26 |
EP0382433A2 (en) | 1990-08-16 |
FI900602A0 (fi) | 1990-02-07 |
ATE159984T1 (de) | 1997-11-15 |
AU4902590A (en) | 1990-08-16 |
IE900442L (en) | 1990-08-07 |
EP0382433A3 (en) | 1992-01-15 |
GB2228998A (en) | 1990-09-12 |
CA2009454C (en) | 2000-04-04 |
ZA90912B (en) | 1990-10-31 |
NO900582L (no) | 1990-08-08 |
NO900582D0 (no) | 1990-02-07 |
GB2228998B (en) | 1993-04-07 |
GB9002536D0 (en) | 1990-04-04 |
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