ES2109226T3 - Deteccion de secuencias de acidos nucleicos usando polarizacion de fluorescencia. - Google Patents

Deteccion de secuencias de acidos nucleicos usando polarizacion de fluorescencia.

Info

Publication number
ES2109226T3
ES2109226T3 ES90301135T ES90301135T ES2109226T3 ES 2109226 T3 ES2109226 T3 ES 2109226T3 ES 90301135 T ES90301135 T ES 90301135T ES 90301135 T ES90301135 T ES 90301135T ES 2109226 T3 ES2109226 T3 ES 2109226T3
Authority
ES
Spain
Prior art keywords
nucleic acid
detection
acid sequences
fluorescence polarization
detect
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90301135T
Other languages
English (en)
Inventor
Andrew John Garman
Robert Stanley Moore
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syngenta Ltd
Original Assignee
Zeneca Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeneca Ltd filed Critical Zeneca Ltd
Application granted granted Critical
Publication of ES2109226T3 publication Critical patent/ES2109226T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

METODO DE ENSAYO PARA LA DETECCION DE SECUENCIAS DE ACIDO NUCLEICO EN SOLUCIONES HOMOGENEAS. EL METODO COMPRENDE EL USO DE POLARIZACION FLUORESCENTE PARA DETECTAR LA HIBRIDACION DE UNA MUESTRA DE ACIDO NUCLEICO FLUORESCENTE O PARA DETECTAR PRODUCTOS DE PRIMERA EXTENSION FLUORESCENTE. EQUIPO DE ENSAYO PARA USAR EN EL METODO ANTERIOR.
ES90301135T 1989-02-07 1990-02-02 Deteccion de secuencias de acidos nucleicos usando polarizacion de fluorescencia. Expired - Lifetime ES2109226T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB898902689A GB8902689D0 (en) 1989-02-07 1989-02-07 Assay method

Publications (1)

Publication Number Publication Date
ES2109226T3 true ES2109226T3 (es) 1998-01-16

Family

ID=10651255

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90301135T Expired - Lifetime ES2109226T3 (es) 1989-02-07 1990-02-02 Deteccion de secuencias de acidos nucleicos usando polarizacion de fluorescencia.

Country Status (14)

Country Link
US (1) US6022686A (es)
EP (1) EP0382433B1 (es)
JP (1) JPH02295496A (es)
AT (1) ATE159984T1 (es)
AU (1) AU4902590A (es)
CA (1) CA2009454C (es)
DE (1) DE69031665T2 (es)
ES (1) ES2109226T3 (es)
FI (1) FI900602A0 (es)
GB (2) GB8902689D0 (es)
IE (1) IE900442L (es)
IL (1) IL93297A0 (es)
NO (1) NO900582L (es)
ZA (1) ZA90912B (es)

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Also Published As

Publication number Publication date
GB2228998A (en) 1990-09-12
US6022686A (en) 2000-02-08
IL93297A0 (en) 1990-11-29
GB9002536D0 (en) 1990-04-04
NO900582L (no) 1990-08-08
GB8902689D0 (en) 1989-03-30
EP0382433A2 (en) 1990-08-16
GB2228998B (en) 1993-04-07
ZA90912B (en) 1990-10-31
DE69031665T2 (de) 1998-02-26
EP0382433B1 (en) 1997-11-05
CA2009454C (en) 2000-04-04
CA2009454A1 (en) 1990-08-07
AU4902590A (en) 1990-08-16
IE900442L (en) 1990-08-07
JPH02295496A (ja) 1990-12-06
EP0382433A3 (en) 1992-01-15
FI900602A0 (fi) 1990-02-07
NO900582D0 (no) 1990-02-07
ATE159984T1 (de) 1997-11-15
DE69031665D1 (de) 1997-12-11

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