DE69532255D1 - Verfahren zum Nachweis von einer Zielsubstanz in einer Probe mit Hilfe von Pyryliumverbindung - Google Patents

Verfahren zum Nachweis von einer Zielsubstanz in einer Probe mit Hilfe von Pyryliumverbindung

Info

Publication number
DE69532255D1
DE69532255D1 DE69532255T DE69532255T DE69532255D1 DE 69532255 D1 DE69532255 D1 DE 69532255D1 DE 69532255 T DE69532255 T DE 69532255T DE 69532255 T DE69532255 T DE 69532255T DE 69532255 D1 DE69532255 D1 DE 69532255D1
Authority
DE
Germany
Prior art keywords
target substance
sample
detection
reagents
interaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69532255T
Other languages
English (en)
Inventor
Nobuko Yamamoto
Tadashi Okamoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Canon Inc
Original Assignee
Canon Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP11262694A external-priority patent/JP3796271B2/ja
Priority claimed from JP12504094A external-priority patent/JP4114958B2/ja
Application filed by Canon Inc filed Critical Canon Inc
Application granted granted Critical
Publication of DE69532255D1 publication Critical patent/DE69532255D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D309/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings
    • C07D309/34—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
DE69532255T 1994-05-26 1995-05-25 Verfahren zum Nachweis von einer Zielsubstanz in einer Probe mit Hilfe von Pyryliumverbindung Expired - Lifetime DE69532255D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP11262694A JP3796271B2 (ja) 1994-05-26 1994-05-26 試料中の標的物質の検出方法
JP12504094A JP4114958B2 (ja) 1994-06-07 1994-06-07 核酸ハイブリッド体の検出方法及びそれに用いるプローブ

Publications (1)

Publication Number Publication Date
DE69532255D1 true DE69532255D1 (de) 2004-01-22

Family

ID=26451740

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69532255T Expired - Lifetime DE69532255D1 (de) 1994-05-26 1995-05-25 Verfahren zum Nachweis von einer Zielsubstanz in einer Probe mit Hilfe von Pyryliumverbindung

Country Status (4)

Country Link
US (1) US6156506A (de)
EP (1) EP0684239B1 (de)
AT (1) ATE256119T1 (de)
DE (1) DE69532255D1 (de)

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EP0837141B1 (de) * 1996-10-03 2003-01-08 Canon Kabushiki Kaisha Verfahren zur Detektion von Zielnukleinsäure, Verfahren zu ihrer Quantifizierung und Pyrylium-Verbindungen zur Chemilumineszenz-Analyse
US6960432B2 (en) * 1999-07-26 2005-11-01 Canon Kabushiki Kaisha Detection/quantification of targeted nucleotide chains, and detection/quantification of multi-stranded nucleotide chains by fluorescence
US7897140B2 (en) 1999-12-23 2011-03-01 Health Research, Inc. Multi DTPA conjugated tetrapyrollic compounds for phototherapeutic contrast agents
US20050191357A1 (en) * 2002-03-20 2005-09-01 Yoshiaki Kawashima Method of manufacturing chemical-containing composite particles
US10001475B2 (en) 2002-06-20 2018-06-19 The Regents Of The University Of California Light harvesting multichromophore compositions and methods of using the same
US9371559B2 (en) 2002-06-20 2016-06-21 The Regents Of The University Of California Compositions for detection and analysis of polynucleotides using light harvesting multichromophores
US7144950B2 (en) 2003-09-17 2006-12-05 The Regents Of The University Of California Conformationally flexible cationic conjugated polymers
DE60328496D1 (en) 2002-06-27 2009-09-03 Health Research Inc Fluorinierte chlorin und bacteriochlorin photosensitizer für fotodynamische therapie
WO2004092324A2 (en) 2002-08-26 2004-10-28 The Regents Of The University Of California Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
EP1549772B1 (de) * 2002-08-26 2013-01-02 The Regents of The University of California Verfahren und zusammensetzungen zum nachweis und zur analyse von polynukleotiden unter verwendung von licht einfangenden multichromophoren
AU2004215006A1 (en) 2003-02-13 2004-09-10 The Regents Of The University Of California Methods and compositions for detection and analysis of polynucleotide-binding protein interactions using light harvesting multichromophores
JP4458327B2 (ja) * 2003-08-28 2010-04-28 キヤノン株式会社 標的物質の定量方法および該方法に用いるプローブ担体
JP4541731B2 (ja) * 2004-03-12 2010-09-08 キヤノン株式会社 核酸検出方法
US8216610B2 (en) * 2004-05-28 2012-07-10 Imaginot Pty Ltd. Oral paracetamol formulations
JP4183256B2 (ja) * 2004-08-04 2008-11-19 キヤノン株式会社 核酸増幅反応産物の鎖分離方法、核酸増幅反応産物の検出方法
JP2006094760A (ja) * 2004-09-29 2006-04-13 Canon Inc 細胞のスクリーニング方法および口腔癌細胞検出方法
EP1838752B1 (de) 2005-01-10 2017-10-04 The Regents of The University of California Kationische konjugierte polymere zur strangspezifischen polynukleotid-erkennung in homogenen festkörper-testanordnungen
WO2006074482A2 (en) 2005-01-10 2006-07-13 The Regents Of The University Of California Methods and kits for strand-specific polynucleotide detection with cationic multichromophores
US8535914B2 (en) * 2005-01-21 2013-09-17 Canon Kabushiki Kaisha Probe, probe set and information acquisition method using the same
US7666594B2 (en) 2005-01-31 2010-02-23 The Regents Of The University Of California Methods for assaying a sample for an aggregant
JP2007010413A (ja) * 2005-06-29 2007-01-18 Canon Inc 核酸ハイブリッドの融点測定方法及びそのための装置
US7642086B2 (en) * 2005-08-09 2010-01-05 Canon Kabushiki Kaisha Labeled probe bound object, method for producing the same and method for using the same
AU2006317530B2 (en) 2005-11-28 2011-09-01 Imaginot Pty Ltd Oral therapeutic compound delivery system
US20090011413A1 (en) * 2005-12-14 2009-01-08 Canon Kabushiki Kaisha Method for screening colon cancer cells and gene set used for examination of colon cancer
JP5729904B2 (ja) 2009-06-02 2015-06-03 キヤノン株式会社 細胞から蛋白質、ｄｎａ、ｒｎａを調製する方法
WO2011093186A1 (ja) * 2010-01-26 2011-08-04 明治製菓株式会社 ピリピロペン生合成遺伝子群およびマーカー遺伝子を含む核酸構築物
CN113072534B (zh) * 2021-04-02 2022-02-08 广西师范大学 一种rna荧光探针及其制备方法和应用

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US3786369A (en) * 1972-12-27 1974-01-15 Eastman Kodak Co Laser dye composition
US4341894A (en) * 1978-03-13 1982-07-27 Eastman Kodak Company Sensitizers for photoconductive compositions
US4555396A (en) * 1982-12-22 1985-11-26 Eastman Kodak Company Use of pyrylium and thiapyrylium compounds as biological stains
US4840784A (en) * 1982-12-22 1989-06-20 Eastman Kodak Company Use of pyrylium and thiapyrylium compounds as biological stains
US4886744A (en) * 1985-04-25 1989-12-12 Polaroid Corporation Fluorescent conjugates and biological diagnostic assay system
US5011769A (en) * 1985-12-05 1991-04-30 Meiogenics U.S. Limited Partnership Methods for detecting nucleic acid sequences
CA1273552A (en) * 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
EP0232967B1 (de) * 1986-01-10 1993-04-28 Amoco Corporation Kompetitiver homogener Test
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CA1323293C (en) * 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
US5324829A (en) * 1988-12-16 1994-06-28 Ortho Diagnostic Systems, Inc. High specific activity nucleic acid probes having target recognition and signal generating moieties
US5229297A (en) * 1989-02-03 1993-07-20 Eastman Kodak Company Containment cuvette for PCR and method of use
GB8902689D0 (en) * 1989-02-07 1989-03-30 Ici Plc Assay method
CA2033692A1 (en) * 1990-01-25 1991-07-26 Wilhelm Bannwarth Energy transfer systems
US5102784A (en) * 1990-05-04 1992-04-07 Oncor, Inc. Restriction amplification assay
DE69128520T2 (de) * 1990-10-31 1998-07-09 Tosoh Corp Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
JP2788786B2 (ja) * 1991-11-15 1998-08-20 イゲン，インコーポレーテッド 増幅生成物の迅速アッセイ
ATE319853T1 (de) * 1992-11-27 2006-03-15 Canon Kk Verfahren und sonde zum nachweis von nukleinsäuren
JP3247001B2 (ja) * 1992-12-21 2002-01-15 キヤノン株式会社 ピリリウム化合物を用いた２本鎖核酸の検出方法、ピリリウム化合物を含有するプローブ及びそれを用いた標的核酸の検出方法、新規ピリリウム化合物
EP0643140B1 (de) * 1993-09-13 2001-10-31 Canon Kabushiki Kaisha Bestimmung von Nukleinsäuren durch PCR, Messung der Anzahl von mikrobiellen Zellen, Genen oder Genkopien durch PCR und Kit zu ihrer Verwendung
US5591578A (en) * 1993-12-10 1997-01-07 California Institute Of Technology Nucleic acid mediated electron transfer

Also Published As

Publication number Publication date
US6156506A (en) 2000-12-05
EP0684239B1 (de) 2003-12-10
ATE256119T1 (de) 2003-12-15
EP0684239A1 (de) 1995-11-29

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Legal Events

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8332 No legal effect for de