DK0408918T3 - Fremgangsmåde til detektion af nucleinsyrer - Google Patents

Fremgangsmåde til detektion af nucleinsyrer

Info

Publication number
DK0408918T3
DK0408918T3 DK90111773.9T DK90111773T DK0408918T3 DK 0408918 T3 DK0408918 T3 DK 0408918T3 DK 90111773 T DK90111773 T DK 90111773T DK 0408918 T3 DK0408918 T3 DK 0408918T3
Authority
DK
Denmark
Prior art keywords
nucleic acid
probe
detecting
hybrid
nucleic acids
Prior art date
Application number
DK90111773.9T
Other languages
English (en)
Inventor
Kinya Kato
Harumi Iwashita
Nobuko Yamamoto
Masanori Sakuranaga
Original Assignee
Canon Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Canon Kk filed Critical Canon Kk
Application granted granted Critical
Publication of DK0408918T3 publication Critical patent/DK0408918T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/172Haplotypes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DK90111773.9T 1989-06-23 1990-06-21 Fremgangsmåde til detektion af nucleinsyrer DK0408918T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP15971889 1989-06-23
JP1303182A JP2802125B2 (ja) 1989-06-23 1989-11-24 核酸の検出方法

Publications (1)

Publication Number Publication Date
DK0408918T3 true DK0408918T3 (da) 1993-12-06

Family

ID=26486428

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90111773.9T DK0408918T3 (da) 1989-06-23 1990-06-21 Fremgangsmåde til detektion af nucleinsyrer

Country Status (6)

Country Link
EP (1) EP0408918B1 (da)
JP (1) JP2802125B2 (da)
AT (1) ATE97452T1 (da)
DE (1) DE69004632T2 (da)
DK (1) DK0408918T3 (da)
ES (1) ES2059894T3 (da)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9208000D0 (en) * 1992-04-10 1992-05-27 Univ London Quantitative viral assay
ES2133529T3 (es) * 1992-11-27 1999-09-16 Innogenetics Nv Procedimiento para tipificar aislamientos de hcv.
SG63589A1 (en) * 1993-05-14 1999-03-30 Johnson & Johnson Clin Diag Diagnostic compositions elements methods and test kits for amplification and detection of two or more dna's using primers having matched melting temperatures
US7803529B1 (en) 1995-04-11 2010-09-28 Sequenom, Inc. Solid phase sequencing of biopolymers
JP2000513202A (ja) * 1995-09-15 2000-10-10 ジェンザイム・コーポレーション 核酸の塩基配列決定または遺伝的置換の大量スクリーニング法
NO972006D0 (no) 1997-04-30 1997-04-30 Forskningsparken I Aas As Ny metode for diagnose av sykdommer
US6566101B1 (en) 1997-06-16 2003-05-20 Anthony P. Shuber Primer extension methods for detecting nucleic acids
US5888778A (en) * 1997-06-16 1999-03-30 Exact Laboratories, Inc. High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers
WO1999023256A1 (en) 1997-10-30 1999-05-14 Cold Spring Harbor Laboratory Probe arrays and methods of using probe arrays for distinguishing dna
EP1121469A2 (de) * 1998-10-12 2001-08-08 Attomol GmbH Molekulare Diagnostika Verfahren und kit zum direkten nachweis von nukleotidsequenzen, aminosäuresequenzen oder antigenen
US6503718B2 (en) 1999-01-10 2003-01-07 Exact Sciences Corporation Methods for detecting mutations using primer extension for detecting disease
US6280947B1 (en) 1999-08-11 2001-08-28 Exact Sciences Corporation Methods for detecting nucleotide insertion or deletion using primer extension
EP1159452A1 (fr) * 1999-03-05 2001-12-05 Transgene S.A. FRAGMENT NUCLEOTIDIQUE, SONDE, AMORCE, REACTIF ET PROCEDE DE DETECTION D'UNE SEQUENCE NUCLEOTIDIQUE DERIVEE DE L'ORIGINE DE REPLICATION DE pBR322
WO2001083822A2 (en) * 2000-05-01 2001-11-08 Cold Spring Harbor Laboratory Use of representations of dna for genetic analysis
US6428964B1 (en) 2001-03-15 2002-08-06 Exact Sciences Corporation Method for alteration detection
US7196183B2 (en) 2001-08-31 2007-03-27 Innogenetics N.V. Hepatitis C virus genotype, and its use as prophylactic, therapeutic and diagnostic agent
US7776524B2 (en) 2002-02-15 2010-08-17 Genzyme Corporation Methods for analysis of molecular events
RU2390561C2 (ru) 2003-05-23 2010-05-27 Колд Спринг Харбор Лэборетери Виртуальные наборы фрагментов нуклеотидных последовательностей
ES2596753T3 (es) 2003-08-29 2017-01-11 Fujirebio Europe N.V. Nuevo clado del virus de la hepatitis C y secuencias prototipo del mismo
GB0412301D0 (en) 2004-06-02 2004-07-07 Diagenic As Product and method
US7981607B2 (en) 2004-08-27 2011-07-19 Esoterix Genetic Laboratories LLC Method for detecting recombinant event
WO2006047787A2 (en) 2004-10-27 2006-05-04 Exact Sciences Corporation Method for monitoring disease progression or recurrence
WO2007044071A2 (en) 2005-04-21 2007-04-19 Exact Sciences Corporation Analysis of heterogeneous nucleic acid samples

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4623627A (en) * 1983-08-19 1986-11-18 Cetus Corporation Monoclonal antibody having specificity for the double-stranded conformation of native DNA and diagnostic methods using same
IL73577A (en) * 1983-12-12 1989-10-31 Miles Lab Method and reagent system for detecting dna or rna sequences in a test medium containing single stranded dna or rna using antibodies to intercalation complexes with double stranded nucleic acid
EP0144914A3 (en) * 1983-12-12 1986-08-13 Miles Inc. Hybridization assay employing labeled pairs of hybrid binding reagents
US4820630A (en) * 1984-11-23 1989-04-11 Digene Diagnostics, Incorporated Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels
NO870613L (no) * 1986-03-05 1987-09-07 Molecular Diagnostics Inc Deteksjon av mikroorganismer i en prŸve inneholdende nukleinsyre.
US4851331A (en) * 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase

Also Published As

Publication number Publication date
DE69004632T2 (de) 1994-03-24
DE69004632D1 (de) 1993-12-23
EP0408918A1 (en) 1991-01-23
EP0408918B1 (en) 1993-11-18
ATE97452T1 (de) 1993-12-15
ES2059894T3 (es) 1994-11-16
JP2802125B2 (ja) 1998-09-24
JPH03103199A (ja) 1991-04-30

Similar Documents

Publication Publication Date Title
DK0408918T3 (da) Fremgangsmåde til detektion af nucleinsyrer
DK9586D0 (da) Fotokemisk metode til maerkning af nucleinsyrer til paavisning ved hybridiseringsbestemmelser
DK0407789T3 (da) Fremgangsmåde til detektering af nukleinsyrer
SE8600011D0 (sv) Metod for identifiering av nukleinsyror
DE3480487D1 (en) Nucleic acid hybridization assay
DE3681176D1 (de) Verfahren zur erhoehung der empfindlichkeit von nukleinsaeure-hybridisierungstesten.
RO86356A (ro) Metoda de identificare microbiana
DE3785865D1 (de) Schnellverfahren zum nachweis von nukleinsaeure-sequenzen in einer probe mittels probemarkierung.
DE69532255D1 (de) Verfahren zum Nachweis von einer Zielsubstanz in einer Probe mit Hilfe von Pyryliumverbindung
ATE398186T1 (de) Integriertes verfahren und system zur amplifizierung und zum nachweis von nukleinsäuren
EP0235727A3 (en) Rapid dna test for microbes
AU3535389A (en) Method for diagnosis of infectious diseases and method of detecting and identifying microorganisms
EP0368208A3 (en) Immunoassay method and test kit therefor
EP0267521A3 (en) Method for the detection of nucleic acid hybrids
DE59105402D1 (de) Verfahren zum empfindlichen nachweis von nukleinsäuren.
ATE165117T1 (de) Verfahren zur trennung eines zieloligonucleotids
ATE211770T1 (de) Verfahren zur bestimmung von nukleinsäuren
NO173714C (no) Fremgangsmaate for kvalitativ og kvantitativ bestemmelse av speisielle antigener, spesielt sporer av Clostridium tyrobutyricum