DE69004632D1 - Verfahren zum Nachweis von Nucleinsäuren. - Google Patents
Verfahren zum Nachweis von Nucleinsäuren.Info
- Publication number
- DE69004632D1 DE69004632D1 DE90111773T DE69004632T DE69004632D1 DE 69004632 D1 DE69004632 D1 DE 69004632D1 DE 90111773 T DE90111773 T DE 90111773T DE 69004632 T DE69004632 T DE 69004632T DE 69004632 D1 DE69004632 D1 DE 69004632D1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acid
- probe
- detection
- detecting
- hybrid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/172—Haplotypes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP15971889 | 1989-06-23 | ||
JP1303182A JP2802125B2 (ja) | 1989-06-23 | 1989-11-24 | 核酸の検出方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69004632D1 true DE69004632D1 (de) | 1993-12-23 |
DE69004632T2 DE69004632T2 (de) | 1994-03-24 |
Family
ID=26486428
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE90111773T Expired - Fee Related DE69004632T2 (de) | 1989-06-23 | 1990-06-21 | Verfahren zum Nachweis von Nucleinsäuren. |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0408918B1 (de) |
JP (1) | JP2802125B2 (de) |
AT (1) | ATE97452T1 (de) |
DE (1) | DE69004632T2 (de) |
DK (1) | DK0408918T3 (de) |
ES (1) | ES2059894T3 (de) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9208000D0 (en) * | 1992-04-10 | 1992-05-27 | Univ London | Quantitative viral assay |
DE69334357D1 (de) * | 1992-11-27 | 2011-06-09 | Innogenetics Nv | Nachweis und Typisierung das Hepatitis C Virus (HCV) mittels 5'UTR und NS5 Nukleinsäuresequenzen |
EP0630973A3 (de) * | 1993-05-14 | 1995-04-26 | Eastman Kodak Co | Diagnosezusammensetzungen, Elemente, Verfahren und Testkits zur Amplifikation und zum Nachweis von zwei oder mehren DNS und Verwendung von Primern mit angepassten Schmelztemperaturen. |
US7803529B1 (en) | 1995-04-11 | 2010-09-28 | Sequenom, Inc. | Solid phase sequencing of biopolymers |
EP0789781A1 (de) * | 1995-09-15 | 1997-08-20 | Genzyme Corporation | Untersuchungsverfahren mit hohem umsatz für sequenzen und genetischen abweichungen in nukleinsäuren |
NO972006D0 (no) | 1997-04-30 | 1997-04-30 | Forskningsparken I Aas As | Ny metode for diagnose av sykdommer |
US6566101B1 (en) | 1997-06-16 | 2003-05-20 | Anthony P. Shuber | Primer extension methods for detecting nucleic acids |
US5888778A (en) * | 1997-06-16 | 1999-03-30 | Exact Laboratories, Inc. | High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers |
WO1999023256A1 (en) | 1997-10-30 | 1999-05-14 | Cold Spring Harbor Laboratory | Probe arrays and methods of using probe arrays for distinguishing dna |
AU6471399A (en) * | 1998-10-12 | 2000-05-01 | Attomol Gmbh Molekulare Diagnostika | Method and kit for directly detecting nucleotide sequences, amino acid sequencesor antigens |
US6503718B2 (en) | 1999-01-10 | 2003-01-07 | Exact Sciences Corporation | Methods for detecting mutations using primer extension for detecting disease |
US6280947B1 (en) | 1999-08-11 | 2001-08-28 | Exact Sciences Corporation | Methods for detecting nucleotide insertion or deletion using primer extension |
JP2002537856A (ja) * | 1999-03-05 | 2002-11-12 | トランジェーヌ | ヌクレオチド断片、プローブ、プライマー、試薬、及びpBR322の複製起点由来のヌクレオチド配列を検出する方法 |
AU2001257448A1 (en) * | 2000-05-01 | 2001-11-12 | Cold Spring Harbor Laboratory | Use of representations of dna for genetic analysis |
US6428964B1 (en) | 2001-03-15 | 2002-08-06 | Exact Sciences Corporation | Method for alteration detection |
US7196183B2 (en) | 2001-08-31 | 2007-03-27 | Innogenetics N.V. | Hepatitis C virus genotype, and its use as prophylactic, therapeutic and diagnostic agent |
AU2003213107A1 (en) | 2002-02-15 | 2003-09-09 | Exact Sciences Corporation | Methods for analysis of molecular events |
US8694263B2 (en) | 2003-05-23 | 2014-04-08 | Cold Spring Harbor Laboratory | Method of identifying virtual representations of nucleotide sequences |
US8124747B2 (en) | 2003-08-29 | 2012-02-28 | Innogenetics | HCV clade and prototype sequences thereof |
GB0412301D0 (en) | 2004-06-02 | 2004-07-07 | Diagenic As | Product and method |
US7981607B2 (en) | 2004-08-27 | 2011-07-19 | Esoterix Genetic Laboratories LLC | Method for detecting recombinant event |
US9109256B2 (en) | 2004-10-27 | 2015-08-18 | Esoterix Genetic Laboratories, Llc | Method for monitoring disease progression or recurrence |
WO2007044071A2 (en) | 2005-04-21 | 2007-04-19 | Exact Sciences Corporation | Analysis of heterogeneous nucleic acid samples |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4623627A (en) * | 1983-08-19 | 1986-11-18 | Cetus Corporation | Monoclonal antibody having specificity for the double-stranded conformation of native DNA and diagnostic methods using same |
EP0144914A3 (de) * | 1983-12-12 | 1986-08-13 | Miles Inc. | Hybridisierungstest mittels markierter Paare von hybridbindenden Reagenzien |
IL73577A (en) * | 1983-12-12 | 1989-10-31 | Miles Lab | Method and reagent system for detecting dna or rna sequences in a test medium containing single stranded dna or rna using antibodies to intercalation complexes with double stranded nucleic acid |
US4820630A (en) * | 1984-11-23 | 1989-04-11 | Digene Diagnostics, Incorporated | Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels |
NO870613L (no) * | 1986-03-05 | 1987-09-07 | Molecular Diagnostics Inc | Deteksjon av mikroorganismer i en prve inneholdende nukleinsyre. |
US4851331A (en) * | 1986-05-16 | 1989-07-25 | Allied Corporation | Method and kit for polynucleotide assay including primer-dependant DNA polymerase |
-
1989
- 1989-11-24 JP JP1303182A patent/JP2802125B2/ja not_active Expired - Fee Related
-
1990
- 1990-06-21 DE DE90111773T patent/DE69004632T2/de not_active Expired - Fee Related
- 1990-06-21 AT AT90111773T patent/ATE97452T1/de not_active IP Right Cessation
- 1990-06-21 EP EP90111773A patent/EP0408918B1/de not_active Expired - Lifetime
- 1990-06-21 ES ES90111773T patent/ES2059894T3/es not_active Expired - Lifetime
- 1990-06-21 DK DK90111773.9T patent/DK0408918T3/da active
Also Published As
Publication number | Publication date |
---|---|
DK0408918T3 (da) | 1993-12-06 |
EP0408918B1 (de) | 1993-11-18 |
ES2059894T3 (es) | 1994-11-16 |
DE69004632T2 (de) | 1994-03-24 |
EP0408918A1 (de) | 1991-01-23 |
ATE97452T1 (de) | 1993-12-15 |
JPH03103199A (ja) | 1991-04-30 |
JP2802125B2 (ja) | 1998-09-24 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |