ATE394508T1 - Late-pcr - Google Patents

Late-pcr

Info

Publication number
ATE394508T1
ATE394508T1 AT02795963T AT02795963T ATE394508T1 AT E394508 T1 ATE394508 T1 AT E394508T1 AT 02795963 T AT02795963 T AT 02795963T AT 02795963 T AT02795963 T AT 02795963T AT E394508 T1 ATE394508 T1 AT E394508T1
Authority
AT
Austria
Prior art keywords
primer
assays
pcr
amplification
low
Prior art date
Application number
AT02795963T
Other languages
English (en)
Inventor
Lawrence Wangh
Kenneth Pierce
Cristina Hartshorn
John Rice
J Sanchez
Original Assignee
Univ Brandeis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Brandeis filed Critical Univ Brandeis
Application granted granted Critical
Publication of ATE394508T1 publication Critical patent/ATE394508T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Diaphragms For Electromechanical Transducers (AREA)
  • Debugging And Monitoring (AREA)
  • Separation By Low-Temperature Treatments (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Saccharide Compounds (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Underground Structures, Protecting, Testing And Restoring Foundations (AREA)
AT02795963T 2001-12-19 2002-12-19 Late-pcr ATE394508T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34188601P 2001-12-19 2001-12-19
US10/320,893 US7198897B2 (en) 2001-12-19 2002-12-17 Late-PCR

Publications (1)

Publication Number Publication Date
ATE394508T1 true ATE394508T1 (de) 2008-05-15

Family

ID=26982708

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02795963T ATE394508T1 (de) 2001-12-19 2002-12-19 Late-pcr

Country Status (12)

Country Link
US (3) US7198897B2 (de)
EP (1) EP1468114B1 (de)
JP (1) JP4355211B2 (de)
AT (1) ATE394508T1 (de)
AU (1) AU2002360685A1 (de)
CA (1) CA2511128C (de)
DE (1) DE60226500D1 (de)
DK (1) DK1468114T3 (de)
ES (1) ES2306807T3 (de)
HK (1) HK1120088A1 (de)
PT (1) PT1468114E (de)
WO (1) WO2003054233A1 (de)

Families Citing this family (116)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030108919A1 (en) * 2001-09-05 2003-06-12 Perlegen Sciences, Inc. Methods for amplification of nucleic acids
EP2159285B1 (de) 2003-01-29 2012-09-26 454 Life Sciences Corporation Verfahren zur Verstärkung und Sequenzierung von Nukleinsäuren
EP1454994A1 (de) * 2003-03-07 2004-09-08 Université de la Méditerranée Standardisiertes und optimiertes quantitatives Reverse-Transkriptase-Echtzeit-Polymerasekettenreaktionsverfahren zum Nachweis von minimalen Resterkrankungen bei Leukämie
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
WO2004098386A2 (en) 2003-05-01 2004-11-18 Gen-Probe Incorporated Oligonucleotides comprising a molecular switch
JP4836795B2 (ja) * 2003-05-19 2011-12-14 ブランデイズ ユニバーシティー 核酸プロセシング方法、キット、及び装置
EP1641809B2 (de) 2003-07-05 2018-10-03 The Johns Hopkins University Verfahren und zusammensetzungen zum nachweis und zur auflistung genetischer variationen
EP1735458B1 (de) * 2004-01-28 2013-07-24 454 Life Sciences Corporation Nukleinsäureamplifikation mit durchlaufemulsion
JP4455168B2 (ja) * 2004-05-31 2010-04-21 株式会社ニチレイフーズ Ｄｎａ増幅方法
EP1776482A2 (de) 2004-06-30 2007-04-25 Applera Corporation Loglineare verstärkung
JP2006055065A (ja) * 2004-08-19 2006-03-02 Toyo Kohan Co Ltd 非対称ｐｃｒを用いた核酸の検出方法、ポリヌクレオチドプローブ、それを固定するプローブ固定化担体およびキット
CN1280422C (zh) * 2004-08-26 2006-10-18 北京博奥生物芯片有限责任公司 一种不对称pcr扩增方法及其应用
ES2359322T3 (es) * 2004-10-18 2011-05-20 Brandeis University Procedimiento para la amplificación de ácidos nucleicos.
EP1802774B1 (de) 2004-10-18 2012-01-11 Brandeis University Reagenzien und verfahren zur verbesserung der reproduzierbarkeit und zur reduzierung des mispriming bei der pcr-amplifikation
EP1828220A4 (de) * 2004-11-12 2009-02-25 Transgenomic Inc Verfahren und zusammensetzungen zum hochempfindlichen nachweis fluoreszenter mutationen mit mismatch-cutting dna-endonukleasen
EP1907588B1 (de) 2005-07-26 2013-06-05 The University of Medicine and Dentistry of New Jersey Tests auf resistenz zu arzneistoffen der echinocandin-klasse
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US8637436B2 (en) 2006-08-24 2014-01-28 California Institute Of Technology Integrated semiconductor bioarray
WO2007141700A2 (en) * 2006-06-08 2007-12-13 Koninklijke Philips Electronics N. V. Microelectronic sensor device for dna detection
US7972786B2 (en) * 2006-07-07 2011-07-05 Brandeis University Detection and analysis of influenza virus
EP2046989B1 (de) 2006-07-17 2013-03-20 Brandeis University Spezialisierte oligonukleotide und deren verwendung bei nukleinsäureamplifikation und -nachweis
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
JP2008154467A (ja) * 2006-12-21 2008-07-10 Olympus Corp 核酸の増幅方法とこれを用いた核酸の解析方法
CN103952470B (zh) * 2007-03-28 2017-11-10 信号诊断公司 高解析度分析核酸以检测序列变异的系统和方法
JP2009148245A (ja) * 2007-06-12 2009-07-09 Toyobo Co Ltd 核酸の迅速な検出方法
GB0720264D0 (en) * 2007-10-17 2007-11-28 Smiths Detection Watford Ltd Sample preparation devices and analyzers
WO2009062166A2 (en) * 2007-11-08 2009-05-14 University Of Washington Dna microarray based identification and mapping of balanced translocation breakpoints
EP2138588A1 (de) * 2008-06-23 2009-12-30 Koninklijke Philips Electronics N.V. Messung der Schmelzkurve während der Amplifizierung
CN102177250B (zh) 2008-06-30 2015-05-06 生命科技公司 由粗制核酸样品直接扩增的方法
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US8633015B2 (en) * 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
WO2016007914A1 (en) * 2014-07-10 2016-01-14 Fluoresentric, Inc. Dna amplification technology
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
US9542526B2 (en) * 2009-03-10 2017-01-10 Canon U.S. Life Sciences, Inc. Method and system for temperature correction in thermal melt analysis
AU2010224100B2 (en) 2009-03-12 2015-10-22 Brandeis University Reagents and methods for PCR
EP2456887B1 (de) 2009-07-21 2015-11-25 Gen-Probe Incorporated Verfahren und zusammensetzungen für den quantitativen nachweis von nukleinsäuresequenzen in einem erweiterten dynamischen bereich
AR077841A1 (es) 2009-08-11 2011-09-28 Univ Brandeis Metodos kits y composiciones de deteccion de acido nucleico a multiples temperaturas, con sonda unica
AR077840A1 (es) * 2009-08-11 2011-09-28 Univ Brandeis Ensayos de deteccion e identificacion de especies y tipos de staphylococcus
CA2767056C (en) 2009-09-02 2018-12-04 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
EP2980224B1 (de) 2009-10-21 2019-07-03 Brandeis University Kits zur analyse einzelstrangiger nukleinsäuresequenzen
EP2550528B1 (de) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Tropfenerzeugung für tropfenbasierte prüfungen
EP2550351A4 (de) 2010-03-25 2014-07-09 Quantalife Inc Detektionssystem für assays auf tröpfchenbasis
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
EP2854057B1 (de) 2010-05-18 2018-03-07 Natera, Inc. Verfahren zur nichtinvasiven pränatalen Ploidiezuordnung
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
IT1405994B1 (it) * 2010-06-22 2014-02-06 Euroclone Spa Metodo ad elevata sensibilita' per rilevare un acido nucleico target in un campione
WO2012023054A2 (en) 2010-08-17 2012-02-23 Smiths Detection-Watford Ltd. Ndm-1 polymerase chain reaction (pcr) assay
US9850529B2 (en) 2010-09-08 2017-12-26 Brandeis University Compositions and methods for nucleic acid based diagnostic assays for variable sequence targets
US20130295570A1 (en) * 2010-10-07 2013-11-07 Brandeis University Compositions and methods for nucleic acid based diagnostic assays
CN103429331B (zh) 2010-11-01 2016-09-28 伯乐生命医学产品有限公司 用于形成乳液的系统
WO2012064978A2 (en) 2010-11-10 2012-05-18 Brandeis University Compositions, methods, and kits for detecting and identifying mycobacteria
EP2646575B1 (de) 2010-12-03 2017-05-31 Brandeis University Nachweis von dna-mutationen
WO2012075231A1 (en) 2010-12-03 2012-06-07 Brandeis University Methods and kits for detecting nucleic acid mutants in wild-type populations
EP3839064A1 (de) * 2010-12-27 2021-06-23 Abbott Molecular Inc. Systeme zur quantifizierung von proben mit hohem titer mittels digitaler pcr
WO2012095748A2 (en) 2011-01-14 2012-07-19 Smiths Detection - Watford Limited Single tube quantitative polymerase chain reaction (pcr)
JP6153874B2 (ja) 2011-02-09 2017-06-28 ナテラ， インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
WO2012129187A1 (en) 2011-03-18 2012-09-27 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
EP2702175B1 (de) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Verfahren und zusammensetzungen zur nukleinsäureanalyse
WO2013019751A1 (en) 2011-07-29 2013-02-07 Bio-Rad Laboratories, Inc., Library characterization by digital assay
US9175337B2 (en) 2011-09-21 2015-11-03 Gen-Probe Incorporated Methods for amplifying nucleic acid using tag-mediated displacement
US9121051B2 (en) 2011-10-31 2015-09-01 Arkray, Inc. Method of determining the abundance of a target nucleotide sequence of a gene of interest
CN104024433B (zh) 2011-10-31 2016-11-16 爱科来株式会社 基因丰度的测定方法
WO2013122453A1 (en) * 2012-02-15 2013-08-22 Universiti Sains Malaysia Device and method for detection of nucleic acid(s)
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
CN105121656A (zh) * 2012-11-15 2015-12-02 以色列分子检测有限公司 Pcr反应混合物及使用其的方法
CN105102467B (zh) 2013-02-07 2019-01-22 新泽西鲁特格斯州立大学 高度选择性核酸扩增引物
WO2014160046A1 (en) * 2013-03-14 2014-10-02 The Trustees Of The University Of Pennsylvania A method for detecting mutations in single cells or single molecules
WO2015023677A1 (en) * 2013-08-12 2015-02-19 Bio-Rad Laboratories, Inc. Amplification reporter with base-pairing oligomers
ES2925313T3 (es) 2013-10-09 2022-10-14 Fluoresentric Inc Método para la detección de secuencias de ácido nucleico diana
WO2015103339A1 (en) * 2013-12-30 2015-07-09 Atreca, Inc. Analysis of nucleic acids associated with single cells using nucleic acid barcodes
CN106460070B (zh) 2014-04-21 2021-10-08 纳特拉公司 检测染色体片段中的突变和倍性
GB201411567D0 (en) 2014-06-30 2014-08-13 Epistem Ltd Quantification methods
US20170335383A1 (en) * 2014-07-24 2017-11-23 Bdrandeis University Linear-expo-linear pcr (lel-pcr)
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
EP4428863A2 (de) 2015-05-11 2024-09-11 Natera, Inc. Verfahren und zusammensetzungen zur bestimmung der ploidie
EA038921B9 (ru) * 2015-05-18 2021-12-06 Сага Диагностикс Аб Обнаружение целевой нуклеиновой кислоты и ее вариантов
US9499861B1 (en) * 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
EP3347714B1 (de) * 2015-09-10 2021-03-10 InSilixa Inc. Verfahren für quantitative multiplex-nukleinsäureamplifikation
WO2017087943A1 (en) 2015-11-20 2017-05-26 Brandeis University Amplifying and detecting coconut cadang-cadang viroid rna
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
EP3440226B1 (de) 2016-04-07 2021-02-17 Rutgers, The State University of New Jersey Zur detektion von nahe verwandten allelen fähiges multiplex-nukleinsäuretestverfahren und reagenzien dafür
US11485996B2 (en) 2016-10-04 2022-11-01 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
US10426424B2 (en) 2017-11-21 2019-10-01 General Electric Company System and method for generating and performing imaging protocol simulations
US12084720B2 (en) 2017-12-14 2024-09-10 Natera, Inc. Assessing graft suitability for transplantation
CA3090426A1 (en) 2018-04-14 2019-10-17 Natera, Inc. Methods for cancer detection and monitoring by means of personalized detection of circulating tumor dna
EP3802857A4 (de) * 2018-06-07 2022-03-09 BioFire Diagnostics, LLC Schnelle thermocycling-verfahren
US11525159B2 (en) 2018-07-03 2022-12-13 Natera, Inc. Methods for detection of donor-derived cell-free DNA
EP3937780A4 (de) 2019-03-14 2022-12-07 InSilixa, Inc. Verfahren und systeme zur zeitgesteuerten fluoreszenzbasierten detektion
CA3150825A1 (en) 2019-10-02 2021-04-08 Fred Russell Kramer Assay methods and kits for detecting rare sequence variants

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5627054A (en) 1996-04-05 1997-05-06 The United States Of America As Represented By The Secretary Of The Army Competitor primer asymmetric polymerase chain reaction
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
US6333179B1 (en) 1997-06-20 2001-12-25 Affymetrix, Inc. Methods and compositions for multiplex amplification of nucleic acids
US6391544B1 (en) * 1998-05-15 2002-05-21 Abbott Laboratories Method for using unequal primer concentrations for generating nucleic acid amplification products
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
EP2365096A1 (de) * 2000-03-22 2011-09-14 Life Technologies Corporation Verfahren zur Verwendung von Halbleiternanokristallen in Nukleinsäureassays auf Kügelchenbasis
US6887664B2 (en) 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR

Also Published As

Publication number Publication date
PT1468114E (pt) 2008-08-19
DE60226500D1 (en) 2008-06-19
EP1468114B1 (de) 2008-05-07
US20080280292A1 (en) 2008-11-13
DK1468114T3 (da) 2008-09-01
JP4355211B2 (ja) 2009-10-28
AU2002360685A1 (en) 2003-07-09
EP1468114A4 (de) 2005-05-25
US9476092B2 (en) 2016-10-25
EP1468114A1 (de) 2004-10-20
US8367325B2 (en) 2013-02-05
JP2005512577A (ja) 2005-05-12
CA2511128C (en) 2011-01-18
WO2003054233A1 (en) 2003-07-03
US20040053254A1 (en) 2004-03-18
ES2306807T3 (es) 2008-11-16
HK1120088A1 (en) 2009-03-20
CA2511128A1 (en) 2003-07-03
US20130210656A1 (en) 2013-08-15
US7198897B2 (en) 2007-04-03

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