AR077840A1 - Ensayos de deteccion e identificacion de especies y tipos de staphylococcus - Google Patents
Ensayos de deteccion e identificacion de especies y tipos de staphylococcusInfo
- Publication number
- AR077840A1 AR077840A1 ARP100102929A ARP100102929A AR077840A1 AR 077840 A1 AR077840 A1 AR 077840A1 AR P100102929 A ARP100102929 A AR P100102929A AR P100102929 A ARP100102929 A AR P100102929A AR 077840 A1 AR077840 A1 AR 077840A1
- Authority
- AR
- Argentina
- Prior art keywords
- type
- detection
- types
- fema
- allow
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Abstract
Se proveen en la presente métodos, kits y composiciones relacionadas con la deteccion de ácido nucleico que permiten la deteccion y discriminacion de diversas especies y tipos de Staphylococcus. En particular, se proveen en la presente ensayos que permiten la deteccion de MSSA, MSSE, MRSA, MRSE, VRSA, VRSE, y cepa MRSA de la comunidad mediante la deteccion de la presencia o ausencia de una o más secuencias diana seleccionadas a partir de una lista de genes y secuencias diana que incluyen, pero no están limitados a: mecA, femA-SA, femA-SE, lukF-PV, IukS-PV, VanA, SCCmec tipo I, tipo II, tipo III, tipo IV, tipo V, y tipo VII. En ciertas realizaciones, se emplean métodos de amplificacion por PCR asimétrica. En otras realizaciones, se detectan senales generadas por sonda-diana a al menos dos temperaturas para generar una relacion de senales temperatura/temperatura.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US23299409P | 2009-08-11 | 2009-08-11 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR077840A1 true AR077840A1 (es) | 2011-09-28 |
Family
ID=43586817
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP100102929A AR077840A1 (es) | 2009-08-11 | 2010-08-10 | Ensayos de deteccion e identificacion de especies y tipos de staphylococcus |
Country Status (4)
Country | Link |
---|---|
US (1) | US9376723B2 (es) |
EP (1) | EP2464752B1 (es) |
AR (1) | AR077840A1 (es) |
WO (1) | WO2011019836A2 (es) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2606151A2 (en) * | 2010-08-17 | 2013-06-26 | Smiths Detection-Watford Limited | Ndm-1 polymerase chain reaction (pcr) assay |
US9850529B2 (en) * | 2010-09-08 | 2017-12-26 | Brandeis University | Compositions and methods for nucleic acid based diagnostic assays for variable sequence targets |
US20150275276A1 (en) * | 2012-11-15 | 2015-10-01 | Molecular Detection Israel Ltd. | Pcr reaction mixtures and methods of using same |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5066584A (en) | 1988-09-23 | 1991-11-19 | Cetus Corporation | Methods for generating single stranded dna by the polymerase chain reaction |
EP0892808B1 (en) * | 1996-04-12 | 2008-05-14 | PHRI Properties, Inc. | Detection probes, kits and assays |
CA2348042A1 (en) | 2001-06-04 | 2002-12-04 | Ann Huletsky | Sequences for detection and identification of methicillin-resistant staphylococcus aureus |
US7198897B2 (en) * | 2001-12-19 | 2007-04-03 | Brandeis University | Late-PCR |
EP2927238B1 (en) * | 2004-10-18 | 2018-01-17 | Brandeis University | Methods for LATE amplification and sequencing of a nucleic acid |
WO2006102695A1 (en) | 2005-04-01 | 2006-10-05 | Medvet Science Pty Ltd | Identifying respiratory infectious organisms |
US20060252069A1 (en) | 2005-04-21 | 2006-11-09 | Zhang Kunyan | Pcr for mrsa sccmec typing |
EP2385140B1 (en) * | 2005-07-25 | 2014-07-16 | Alere San Diego, Inc. | Methods for multiplexing recombinase polymerase amplification |
US8535888B2 (en) | 2006-12-29 | 2013-09-17 | Mayo Foundation For Medical Education And Research | Compositions and methods for detecting methicillin-resistant S. aureus |
AR077841A1 (es) * | 2009-08-11 | 2011-09-28 | Univ Brandeis | Metodos kits y composiciones de deteccion de acido nucleico a multiples temperaturas, con sonda unica |
-
2010
- 2010-08-10 AR ARP100102929A patent/AR077840A1/es unknown
- 2010-08-11 US US13/389,927 patent/US9376723B2/en active Active
- 2010-08-11 WO PCT/US2010/045198 patent/WO2011019836A2/en active Application Filing
- 2010-08-11 EP EP10808707.3A patent/EP2464752B1/en not_active Not-in-force
Also Published As
Publication number | Publication date |
---|---|
EP2464752A4 (en) | 2013-03-06 |
US20120208191A1 (en) | 2012-08-16 |
WO2011019836A3 (en) | 2011-04-14 |
US9376723B2 (en) | 2016-06-28 |
EP2464752B1 (en) | 2017-04-12 |
EP2464752A2 (en) | 2012-06-20 |
WO2011019836A2 (en) | 2011-02-17 |
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